arfgef1 cDNA ORF clone, Danio rerio(zebrafish)

The following arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa49262 XM_009297394.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49263 XM_009297395.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49264 XM_009297396.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49265 XM_009297397.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49266 XM_009297398.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49267 XM_009297399.1
Latest version!
Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa49262
    Clone ID Related Accession (Same CDS sequence) XM_009297394.1
    Accession Version XM_009297394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA
    AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC
    AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT
    AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG
    CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC
    GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA
    CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC
    AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG
    ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC
    GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG
    TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG
    TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC
    ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC
    CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA
    CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA
    TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC
    AACCCCAATT CCCAGACAAG TCTTGGGAGA GCAGGCCAAG AAAAGCCTTC AGAGCAGGAG
    AGCAATGAGT CGAAGCATCC AGAAACGATC AACCGTTACG GCAGCATTAA CTCTTTGGAC
    TCCACTGCAT CATCAGGCAT TGGGAGCTAC AGCACACAGA TGTCCGGAAC AGACAACCCG
    GAGCAGTTTG AGGTCCTCAA ACAACAGAAG GAGATCATAG AGCAAGGCAT TGACCTGTTC
    AACAAAAAAC CAAAGAGGGG CATTCAGTAC CTGCAGGAGC AAGGCATGCT GGGAACAACG
    CCTGAAGATA TTGCCCAGTT TTTACATCAA GAGGAGAGAC TTGACTCTAT CCAGGTAGGA
    GAGTTTCTAG GAGACAACGA CCGCATCAAT AAAGAGGTCA TGTATGCCTA CGTCGACCAA
    ATGGACTTCC AGGGCAAAGA CTTCGTGCCA GCGCTGCGCA TGTTCCTGGA GGGTTTCCGT
    CTGCCCGGCG AAGCCCAGAA AATTGATCGA CTGATGGAGA AATTTGCTGC TAGATATTTA
    GAATGCAACC AAGGGCAAAC ACTGTTCGCC AGCGCTGACA CCGCATACGT TCTTGCGTAT
    TCAATCATCA TGTTGACTAC CGATCTGCAC AGTCCACAGG TGAAGAATAA AATGACGAAA
    GAGCAATACA TCAAGATGAA TCGAGGTATC AATGACAGTA AAGATTTACC GGAGGAGTAT
    CTCTCAGCCA TTTATGATGA AATCGCAGGG AAGAAAATCT CCATGAAGGA GACAAAGGAG
    TTAACTCTCA AATCAAATAA GCAAAGTGTG GCGAGTGAGA AGCAGAGGAG GCTGCTGTAT
    AATGTTGAGA TGGAGCAGAT GGCAAAAACA GCAAAGGCTC TGATGGAGGC CGTGAGCCAC
    GTTCAAGCCC CCTTCACCAG CGCCACACAT CTTGAGCATG TCAGACCAAT GTTTAAGCTG
    GCCTGGACGC CCTTTCTGGC TGCTTTCAGT GTGGGTCTGC AGGACTGTGA TGACACTGAA
    GTGGCCTCTC TGTGTCTGGA GGGAATCCGC TGTGCCATCA GGATAGCATG CATCTTCAGC
    ATTCAGTTGG AGCGAGACGC ATATGTACAG GCTCTGGCCC GTTTCACACT CTTGACGGCC
    AGTTCTGGCA TCACTGAAAT GAAGCAGAAG AACATCGACA CCATCAAGAC CCTCATCACA
    GTGGCTCACA CTGATGGAAA TTATCTAGGA AACTCCTGGC ATGAGATCAT GAAGTGCATT
    AGTCAGCTGG AGTTGGCCCA GCTCATCGGG ACAGGAGTGA AGGCTCGGTA CATATCAGGA
    ACTGTTCGGG GAAAGGAAGG CTTTATCACC AGCACCAAGG AACAAACGTC TGATGAATAC
    CTGGGATTGG GAGGGAATGT TGATCGCAAA CAGATTGCAA GCATTCAGGA GTCTATCGGG
    GAGACCAGCT CGCAAAGTGT AGTTGTTGCT GTAGACCGGA TATTCACGGG CTCTACCAGA
    CTTGATGGAA ATGCCATTGT GGACTTTGTG CGATGGCTGT GCGCTGTCTC GATGGATGAA
    CTGGCCTCCC CAACACACCC TCGAATGTTC AGTCTGCAGA AGATCGTGGA GATTTCATAC
    TACAATATGG GTCGTATCAG GTTGCAGTGG TCCAGGATTT GGGAGGTTAT AGGGGATCAT
    TTCAATAAGG TTGGCTGTAA TCCCAACGAA GATGTTGCTA TTTTTGCTGT GGATTCTCTG
    AGGCAGTTGT CCATGAAGTT TTTGGAAAAG GGAGAGTTGG CCAATTTCAG ATTCCAGAAG
    GATTTCCTCA GGCCGTTCGA GCACATAATG AAAAAGAACA GATCTCCGAC CATTAGAGAC
    ATGGTGGTTC GCTGTATTGC CCAGATGGTC AACTCTCAGG CTGGCAATAT TAGATCAGGG
    TGGAAGAACA TTTTCTCTGT GTTTCATCTG GCTGCTTCTG ATCAGGATGA GAGTATAGTT
    GAGCTGGCCT TCCAGACAAC TGGACACATC GTCACGAATG TATTTGAGAA GCATTTCCCA
    GCTACCATTG ACTCCTTCCA GGACGCTGTC AAGTGTTTGT CTGAGTTCGC CTGCAACGCT
    TCTTTTCCAG ACACCAGCAT GGAGGCTATT CGTCTGATCC GCCACTGCGC CAAATATGTG
    TCAGACCGAC CCCAGGCGTT TAAAGACTAT ACCAGTGATG ATATGAATGT TGCCCCGGAG
    GACCGTGTTT GGGTCAGAGG CTGGTTCCCC ATCCTTTTTG AGCTGTCATG CATCATCAAC
    AGATGCAAAC TGGACGTCCG GACCAGGGGT CTGACAGTGA TGTTTGAGGT GATGAAGACG
    TACGGTCATA CCTATGAGAA ACACTGGTGG CAGGATCTGT TCCGCATCGT CTTCAGGATC
    TTCGACAACA TGAAGCTTCC TGAGCAACAG ACCGAGAAAG CGGAGTGGAT GACCACCACC
    TGCAATCATG CACTTTATGC CATCTGTGAC GTTTTTACAC AGTACTTTGA GTCTCTCAGT
    GATGTTCTTT TAGATGATAT CTTATCTCAG CTCTACTGGT GTGTCCAGCA AGATAATGAG
    CAATTAGCCA GATCAGGCAC TAACTGCCTT GAAAACGTGG TCATCCTTAA TGGTGAGAAG
    TTCAATCCTG AGACTTGGGA CAAGACATGC AACTGCATGC TGGACATCTT CAAAACCACC
    ATCCCACACA TGCTGCTGAC TTGGAGACCG GCTGGTGCTG AAGGAGATCA CATGACTCAG
    CTGGAGTCTG ATAAACAGCT GGACTCCATT TCCCAGAAGT CAGTGGATAT TCAGACCCGC
    TCAGATGACC AGCAGTCTGT TAACAGTATG GAGAAGGCTT TGGCTGACAA CCGCAGATAC
    AGTCAGTTCA GTGTGGCTTC AGAAGCTCAG GAACAGAGGC TGTTTGCTGC GCTACTCATT
    AAGTGTGTGG TTCAGCTGGA GCTCATTCAG ACCATTGACA ACATCGTCTT CTTTCCTGCT
    ACCAGTAAGA AAGAAGATGC GGAAAACTTT GCGGCTGCAC AGAGAGATGC TTTAGATGCT
    GATATACATG TGGACACACA GGACCAGGGA ATGTATCGCT ATCTGACCTC AGAACAGCTC
    TTCAAACTGC TCGACTGTCT GCTGGAGTCT CATCGATTTG CAAAGGCCTT CAACTCCAAT
    AATGAGCAAA GAACTGCTCT GTGGAAAGCC GGTTTCAAAG GGAAGTCGAA GCCCAATCTG
    CTGAAACAGG AGACGAGCAG CCTGGCGTGT GGTCTGCGCA TCCTCTTCCG CATGTACACA
    GATCCAAGCC GACGGGATGC CTGGGAGGAG GTGCAGAGGA GACTGCTCAA CGTTTGCAGT
    GACGCAGTGG CTTATTTTCT GACCCTCACG TCTGAAAGTC ATCGCGAAGC CTGGACAAAC
    CTTCTGCTGC TCTTCCTGAC CAAAGTGCTG AAGATCAGTG ATGATAGATT CAAGGCTCAT
    GCGTCCCGCT ACTACCCTCT GCTCTGTGAA ATCATGCAGT TCGACCTGAT CCCGGAGCTG
    CGGGCCGTCC TGCGCAAGTT TTTCCTGCGC ATTGGACTGG TTTTCCAGAT CACCAACAGC
    CTGGAAACAA ATCAAACTGT CAGCAACGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295669.1
    CDS206..5818
    Translation

    Target ORF information:

    RefSeq Version XM_009297394.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA
    AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC
    AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT
    AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG
    CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC
    GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA
    CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC
    AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG
    ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC
    GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG
    TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG
    TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC
    ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC
    CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA
    CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA
    TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC
    AACCCCAATT CCCAGACAAG TCTTGGGAGA GCAGGCCAAG AAAAGCCTTC AGAGCAGGAG
    AGCAATGAGT CGAAGCATCC AGAAACGATC AACCGTTACG GCAGCATTAA CTCTTTGGAC
    TCCACTGCAT CATCAGGCAT TGGGAGCTAC AGCACACAGA TGTCCGGAAC AGACAACCCG
    GAGCAGTTTG AGGTCCTCAA ACAACAGAAG GAGATCATAG AGCAAGGCAT TGACCTGTTC
    AACAAAAAAC CAAAGAGGGG CATTCAGTAC CTGCAGGAGC AAGGCATGCT GGGAACAACG
    CCTGAAGATA TTGCCCAGTT TTTACATCAA GAGGAGAGAC TTGACTCTAT CCAGGTAGGA
    GAGTTTCTAG GAGACAACGA CCGCATCAAT AAAGAGGTCA TGTATGCCTA CGTCGACCAA
    ATGGACTTCC AGGGCAAAGA CTTCGTGCCA GCGCTGCGCA TGTTCCTGGA GGGTTTCCGT
    CTGCCCGGCG AAGCCCAGAA AATTGATCGA CTGATGGAGA AATTTGCTGC TAGATATTTA
    GAATGCAACC AAGGGCAAAC ACTGTTCGCC AGCGCTGACA CCGCATACGT TCTTGCGTAT
    TCAATCATCA TGTTGACTAC CGATCTGCAC AGTCCACAGG TGAAGAATAA AATGACGAAA
    GAGCAATACA TCAAGATGAA TCGAGGTATC AATGACAGTA AAGATTTACC GGAGGAGTAT
    CTCTCAGCCA TTTATGATGA AATCGCAGGG AAGAAAATCT CCATGAAGGA GACAAAGGAG
    TTAACTCTCA AATCAAATAA GCAAAGTGTG GCGAGTGAGA AGCAGAGGAG GCTGCTGTAT
    AATGTTGAGA TGGAGCAGAT GGCAAAAACA GCAAAGGCTC TGATGGAGGC CGTGAGCCAC
    GTTCAAGCCC CCTTCACCAG CGCCACACAT CTTGAGCATG TCAGACCAAT GTTTAAGCTG
    GCCTGGACGC CCTTTCTGGC TGCTTTCAGT GTGGGTCTGC AGGACTGTGA TGACACTGAA
    GTGGCCTCTC TGTGTCTGGA GGGAATCCGC TGTGCCATCA GGATAGCATG CATCTTCAGC
    ATTCAGTTGG AGCGAGACGC ATATGTACAG GCTCTGGCCC GTTTCACACT CTTGACGGCC
    AGTTCTGGCA TCACTGAAAT GAAGCAGAAG AACATCGACA CCATCAAGAC CCTCATCACA
    GTGGCTCACA CTGATGGAAA TTATCTAGGA AACTCCTGGC ATGAGATCAT GAAGTGCATT
    AGTCAGCTGG AGTTGGCCCA GCTCATCGGG ACAGGAGTGA AGGCTCGGTA CATATCAGGA
    ACTGTTCGGG GAAAGGAAGG CTTTATCACC AGCACCAAGG AACAAACGTC TGATGAATAC
    CTGGGATTGG GAGGGAATGT TGATCGCAAA CAGATTGCAA GCATTCAGGA GTCTATCGGG
    GAGACCAGCT CGCAAAGTGT AGTTGTTGCT GTAGACCGGA TATTCACGGG CTCTACCAGA
    CTTGATGGAA ATGCCATTGT GGACTTTGTG CGATGGCTGT GCGCTGTCTC GATGGATGAA
    CTGGCCTCCC CAACACACCC TCGAATGTTC AGTCTGCAGA AGATCGTGGA GATTTCATAC
    TACAATATGG GTCGTATCAG GTTGCAGTGG TCCAGGATTT GGGAGGTTAT AGGGGATCAT
    TTCAATAAGG TTGGCTGTAA TCCCAACGAA GATGTTGCTA TTTTTGCTGT GGATTCTCTG
    AGGCAGTTGT CCATGAAGTT TTTGGAAAAG GGAGAGTTGG CCAATTTCAG ATTCCAGAAG
    GATTTCCTCA GGCCGTTCGA GCACATAATG AAAAAGAACA GATCTCCGAC CATTAGAGAC
    ATGGTGGTTC GCTGTATTGC CCAGATGGTC AACTCTCAGG CTGGCAATAT TAGATCAGGG
    TGGAAGAACA TTTTCTCTGT GTTTCATCTG GCTGCTTCTG ATCAGGATGA GAGTATAGTT
    GAGCTGGCCT TCCAGACAAC TGGACACATC GTCACGAATG TATTTGAGAA GCATTTCCCA
    GCTACCATTG ACTCCTTCCA GGACGCTGTC AAGTGTTTGT CTGAGTTCGC CTGCAACGCT
    TCTTTTCCAG ACACCAGCAT GGAGGCTATT CGTCTGATCC GCCACTGCGC CAAATATGTG
    TCAGACCGAC CCCAGGCGTT TAAAGACTAT ACCAGTGATG ATATGAATGT TGCCCCGGAG
    GACCGTGTTT GGGTCAGAGG CTGGTTCCCC ATCCTTTTTG AGCTGTCATG CATCATCAAC
    AGATGCAAAC TGGACGTCCG GACCAGGGGT CTGACAGTGA TGTTTGAGGT GATGAAGACG
    TACGGTCATA CCTATGAGAA ACACTGGTGG CAGGATCTGT TCCGCATCGT CTTCAGGATC
    TTCGACAACA TGAAGCTTCC TGAGCAACAG ACCGAGAAAG CGGAGTGGAT GACCACCACC
    TGCAATCATG CACTTTATGC CATCTGTGAC GTTTTTACAC AGTACTTTGA GTCTCTCAGT
    GATGTTCTTT TAGATGATAT CTTATCTCAG CTCTACTGGT GTGTCCAGCA AGATAATGAG
    CAATTAGCCA GATCAGGCAC TAACTGCCTT GAAAACGTGG TCATCCTTAA TGGTGAGAAG
    TTCAATCCTG AGACTTGGGA CAAGACATGC AACTGCATGC TGGACATCTT CAAAACCACC
    ATCCCACACA TGCTGCTGAC TTGGAGACCG GCTGGTGCTG AAGGAGATCA CATGACTCAG
    CTGGAGTCTG ATAAACAGCT GGACTCCATT TCCCAGAAGT CAGTGGATAT TCAGACCCGC
    TCAGATGACC AGCAGTCTGT TAACAGTATG GAGAAGGCTT TGGCTGACAA CCGCAGATAC
    AGTCAGTTCA GTGTGGCTTC AGAAGCTCAG GAACAGAGGC TGTTTGCTGC GCTACTCATT
    AAGTGTGTGG TTCAGCTGGA GCTCATTCAG ACCATTGACA ACATCGTCTT CTTTCCTGCT
    ACCAGTAAGA AAGAAGATGC GGAAAACTTT GCGGCTGCAC AGAGAGATGC TTTAGATGCT
    GATATACATG TGGACACACA GGACCAGGGA ATGTATCGCT ATCTGACCTC AGAACAGCTC
    TTCAAACTGC TCGACTGTCT GCTGGAGTCT CATCGATTTG CAAAGGCCTT CAACTCCAAT
    AATGAGCAAA GAACTGCTCT GTGGAAAGCC GGTTTCAAAG GGAAGTCGAA GCCCAATCTG
    CTGAAACAGG AGACGAGCAG CCTGGCGTGT GGTCTGCGCA TCCTCTTCCG CATGTACACA
    GATCCAAGCC GACGGGATGC CTGGGAGGAG GTGCAGAGGA GACTGCTCAA CGTTTGCAGT
    GACGCAGTGG CTTATTTTCT GACCCTCACG TCTGAAAGTC ATCGCGAAGC CTGGACAAAC
    CTTCTGCTGC TCTTCCTGAC CAAAGTGCTG AAGATCAGTG ATGATAGATT CAAGGCTCAT
    GCGTCCCGCT ACTACCCTCT GCTCTGTGAA ATCATGCAGT TCGACCTGAT CCCGGAGCTG
    CGGGCCGTCC TGCGCAAGTT TTTCCTGCGC ATTGGACTGG TTTTCCAGAT CACCAACAGC
    CTGGAAACAA ATCAAACTGT CAGCAACGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49263
    Clone ID Related Accession (Same CDS sequence) XM_009297395.1
    Accession Version XM_009297395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA
    AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC
    AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT
    AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG
    CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC
    GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA
    CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC
    AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG
    ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC
    GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG
    TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG
    TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC
    ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC
    CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA
    CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA
    TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC
    AACCCCAATT CCCAGACAAG TCTTGGCCAA GAAAAGCCTT CAGAGCAGGA GAGCAATGAG
    TCGAAGCATC CAGAAACGAT CAACCGTTAC GGCAGCATTA ACTCTTTGGA CTCCACTGCA
    TCATCAGGCA TTGGGAGCTA CAGCACACAG ATGTCCGGAA CAGACAACCC GGAGCAGTTT
    GAGGTCCTCA AACAACAGAA GGAGATCATA GAGCAAGGCA TTGACCTGTT CAACAAAAAA
    CCAAAGAGGG GCATTCAGTA CCTGCAGGAG CAAGGCATGC TGGGAACAAC GCCTGAAGAT
    ATTGCCCAGT TTTTACATCA AGAGGAGAGA CTTGACTCTA TCCAGGTAGG AGAGTTTCTA
    GGAGACAACG ACCGCATCAA TAAAGAGGTC ATGTATGCCT ACGTCGACCA AATGGACTTC
    CAGGGCAAAG ACTTCGTGCC AGCGCTGCGC ATGTTCCTGG AGGGTTTCCG TCTGCCCGGC
    GAAGCCCAGA AAATTGATCG ACTGATGGAG AAATTTGCTG CTAGATATTT AGAATGCAAC
    CAAGGGCAAA CACTGTTCGC CAGCGCTGAC ACCGCATACG TTCTTGCGTA TTCAATCATC
    ATGTTGACTA CCGATCTGCA CAGTCCACAG GTGAAGAATA AAATGACGAA AGAGCAATAC
    ATCAAGATGA ATCGAGGTAT CAATGACAGT AAAGATTTAC CGGAGGAGTA TCTCTCAGCC
    ATTTATGATG AAATCGCAGG GAAGAAAATC TCCATGAAGG AGACAAAGGA GTTAACTCTC
    AAATCAAATA AGCAAAGTGT GGCGAGTGAG AAGCAGAGGA GGCTGCTGTA TAATGTTGAG
    ATGGAGCAGA TGGCAAAAAC AGCAAAGGCT CTGATGGAGG CCGTGAGCCA CGTTCAAGCC
    CCCTTCACCA GCGCCACACA TCTTGAGCAT GTCAGACCAA TGTTTAAGCT GGCCTGGACG
    CCCTTTCTGG CTGCTTTCAG TGTGGGTCTG CAGGACTGTG ATGACACTGA AGTGGCCTCT
    CTGTGTCTGG AGGGAATCCG CTGTGCCATC AGGATAGCAT GCATCTTCAG CATTCAGTTG
    GAGCGAGACG CATATGTACA GGCTCTGGCC CGTTTCACAC TCTTGACGGC CAGTTCTGGC
    ATCACTGAAA TGAAGCAGAA GAACATCGAC ACCATCAAGA CCCTCATCAC AGTGGCTCAC
    ACTGATGGAA ATTATCTAGG AAACTCCTGG CATGAGATCA TGAAGTGCAT TAGTCAGCTG
    GAGTTGGCCC AGCTCATCGG GACAGGAGTG AAGGCTCGGT ACATATCAGG AACTGTTCGG
    GGAAAGGAAG GCTTTATCAC CAGCACCAAG GAACAAACGT CTGATGAATA CCTGGGATTG
    GGAGGGAATG TTGATCGCAA ACAGATTGCA AGCATTCAGG AGTCTATCGG GGAGACCAGC
    TCGCAAAGTG TAGTTGTTGC TGTAGACCGG ATATTCACGG GCTCTACCAG ACTTGATGGA
    AATGCCATTG TGGACTTTGT GCGATGGCTG TGCGCTGTCT CGATGGATGA ACTGGCCTCC
    CCAACACACC CTCGAATGTT CAGTCTGCAG AAGATCGTGG AGATTTCATA CTACAATATG
    GGTCGTATCA GGTTGCAGTG GTCCAGGATT TGGGAGGTTA TAGGGGATCA TTTCAATAAG
    GTTGGCTGTA ATCCCAACGA AGATGTTGCT ATTTTTGCTG TGGATTCTCT GAGGCAGTTG
    TCCATGAAGT TTTTGGAAAA GGGAGAGTTG GCCAATTTCA GATTCCAGAA GGATTTCCTC
    AGGCCGTTCG AGCACATAAT GAAAAAGAAC AGATCTCCGA CCATTAGAGA CATGGTGGTT
    CGCTGTATTG CCCAGATGGT CAACTCTCAG GCTGGCAATA TTAGATCAGG GTGGAAGAAC
    ATTTTCTCTG TGTTTCATCT GGCTGCTTCT GATCAGGATG AGAGTATAGT TGAGCTGGCC
    TTCCAGACAA CTGGACACAT CGTCACGAAT GTATTTGAGA AGCATTTCCC AGCTACCATT
    GACTCCTTCC AGGACGCTGT CAAGTGTTTG TCTGAGTTCG CCTGCAACGC TTCTTTTCCA
    GACACCAGCA TGGAGGCTAT TCGTCTGATC CGCCACTGCG CCAAATATGT GTCAGACCGA
    CCCCAGGCGT TTAAAGACTA TACCAGTGAT GATATGAATG TTGCCCCGGA GGACCGTGTT
    TGGGTCAGAG GCTGGTTCCC CATCCTTTTT GAGCTGTCAT GCATCATCAA CAGATGCAAA
    CTGGACGTCC GGACCAGGGG TCTGACAGTG ATGTTTGAGG TGATGAAGAC GTACGGTCAT
    ACCTATGAGA AACACTGGTG GCAGGATCTG TTCCGCATCG TCTTCAGGAT CTTCGACAAC
    ATGAAGCTTC CTGAGCAACA GACCGAGAAA GCGGAGTGGA TGACCACCAC CTGCAATCAT
    GCACTTTATG CCATCTGTGA CGTTTTTACA CAGTACTTTG AGTCTCTCAG TGATGTTCTT
    TTAGATGATA TCTTATCTCA GCTCTACTGG TGTGTCCAGC AAGATAATGA GCAATTAGCC
    AGATCAGGCA CTAACTGCCT TGAAAACGTG GTCATCCTTA ATGGTGAGAA GTTCAATCCT
    GAGACTTGGG ACAAGACATG CAACTGCATG CTGGACATCT TCAAAACCAC CATCCCACAC
    ATGCTGCTGA CTTGGAGACC GGCTGGTGCT GAAGGAGATC ACATGACTCA GCTGGAGTCT
    GATAAACAGC TGGACTCCAT TTCCCAGAAG TCAGTGGATA TTCAGACCCG CTCAGATGAC
    CAGCAGTCTG TTAACAGTAT GGAGAAGGCT TTGGCTGACA ACCGCAGATA CAGTCAGTTC
    AGTGTGGCTT CAGAAGCTCA GGAACAGAGG CTGTTTGCTG CGCTACTCAT TAAGTGTGTG
    GTTCAGCTGG AGCTCATTCA GACCATTGAC AACATCGTCT TCTTTCCTGC TACCAGTAAG
    AAAGAAGATG CGGAAAACTT TGCGGCTGCA CAGAGAGATG CTTTAGATGC TGATATACAT
    GTGGACACAC AGGACCAGGG AATGTATCGC TATCTGACCT CAGAACAGCT CTTCAAACTG
    CTCGACTGTC TGCTGGAGTC TCATCGATTT GCAAAGGCCT TCAACTCCAA TAATGAGCAA
    AGAACTGCTC TGTGGAAAGC CGGTTTCAAA GGGAAGTCGA AGCCCAATCT GCTGAAACAG
    GAGACGAGCA GCCTGGCGTG TGGTCTGCGC ATCCTCTTCC GCATGTACAC AGATCCAAGC
    CGACGGGATG CCTGGGAGGA GGTGCAGAGG AGACTGCTCA ACGTTTGCAG TGACGCAGTG
    GCTTATTTTC TGACCCTCAC GTCTGAAAGT CATCGCGAAG CCTGGACAAA CCTTCTGCTG
    CTCTTCCTGA CCAAAGTGCT GAAGATCAGT GATGATAGAT TCAAGGCTCA TGCGTCCCGC
    TACTACCCTC TGCTCTGTGA AATCATGCAG TTCGACCTGA TCCCGGAGCT GCGGGCCGTC
    CTGCGCAAGT TTTTCCTGCG CATTGGACTG GTTTTCCAGA TCACCAACAG CCTGGAAACA
    AATCAAACTG TCAGCAACGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295670.1
    CDS206..5809
    Translation

    Target ORF information:

    RefSeq Version XM_009297395.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA
    AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC
    AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT
    AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG
    CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC
    GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA
    CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC
    AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG
    ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC
    GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG
    TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG
    TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC
    ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC
    CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA
    CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA
    TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC
    AACCCCAATT CCCAGACAAG TCTTGGCCAA GAAAAGCCTT CAGAGCAGGA GAGCAATGAG
    TCGAAGCATC CAGAAACGAT CAACCGTTAC GGCAGCATTA ACTCTTTGGA CTCCACTGCA
    TCATCAGGCA TTGGGAGCTA CAGCACACAG ATGTCCGGAA CAGACAACCC GGAGCAGTTT
    GAGGTCCTCA AACAACAGAA GGAGATCATA GAGCAAGGCA TTGACCTGTT CAACAAAAAA
    CCAAAGAGGG GCATTCAGTA CCTGCAGGAG CAAGGCATGC TGGGAACAAC GCCTGAAGAT
    ATTGCCCAGT TTTTACATCA AGAGGAGAGA CTTGACTCTA TCCAGGTAGG AGAGTTTCTA
    GGAGACAACG ACCGCATCAA TAAAGAGGTC ATGTATGCCT ACGTCGACCA AATGGACTTC
    CAGGGCAAAG ACTTCGTGCC AGCGCTGCGC ATGTTCCTGG AGGGTTTCCG TCTGCCCGGC
    GAAGCCCAGA AAATTGATCG ACTGATGGAG AAATTTGCTG CTAGATATTT AGAATGCAAC
    CAAGGGCAAA CACTGTTCGC CAGCGCTGAC ACCGCATACG TTCTTGCGTA TTCAATCATC
    ATGTTGACTA CCGATCTGCA CAGTCCACAG GTGAAGAATA AAATGACGAA AGAGCAATAC
    ATCAAGATGA ATCGAGGTAT CAATGACAGT AAAGATTTAC CGGAGGAGTA TCTCTCAGCC
    ATTTATGATG AAATCGCAGG GAAGAAAATC TCCATGAAGG AGACAAAGGA GTTAACTCTC
    AAATCAAATA AGCAAAGTGT GGCGAGTGAG AAGCAGAGGA GGCTGCTGTA TAATGTTGAG
    ATGGAGCAGA TGGCAAAAAC AGCAAAGGCT CTGATGGAGG CCGTGAGCCA CGTTCAAGCC
    CCCTTCACCA GCGCCACACA TCTTGAGCAT GTCAGACCAA TGTTTAAGCT GGCCTGGACG
    CCCTTTCTGG CTGCTTTCAG TGTGGGTCTG CAGGACTGTG ATGACACTGA AGTGGCCTCT
    CTGTGTCTGG AGGGAATCCG CTGTGCCATC AGGATAGCAT GCATCTTCAG CATTCAGTTG
    GAGCGAGACG CATATGTACA GGCTCTGGCC CGTTTCACAC TCTTGACGGC CAGTTCTGGC
    ATCACTGAAA TGAAGCAGAA GAACATCGAC ACCATCAAGA CCCTCATCAC AGTGGCTCAC
    ACTGATGGAA ATTATCTAGG AAACTCCTGG CATGAGATCA TGAAGTGCAT TAGTCAGCTG
    GAGTTGGCCC AGCTCATCGG GACAGGAGTG AAGGCTCGGT ACATATCAGG AACTGTTCGG
    GGAAAGGAAG GCTTTATCAC CAGCACCAAG GAACAAACGT CTGATGAATA CCTGGGATTG
    GGAGGGAATG TTGATCGCAA ACAGATTGCA AGCATTCAGG AGTCTATCGG GGAGACCAGC
    TCGCAAAGTG TAGTTGTTGC TGTAGACCGG ATATTCACGG GCTCTACCAG ACTTGATGGA
    AATGCCATTG TGGACTTTGT GCGATGGCTG TGCGCTGTCT CGATGGATGA ACTGGCCTCC
    CCAACACACC CTCGAATGTT CAGTCTGCAG AAGATCGTGG AGATTTCATA CTACAATATG
    GGTCGTATCA GGTTGCAGTG GTCCAGGATT TGGGAGGTTA TAGGGGATCA TTTCAATAAG
    GTTGGCTGTA ATCCCAACGA AGATGTTGCT ATTTTTGCTG TGGATTCTCT GAGGCAGTTG
    TCCATGAAGT TTTTGGAAAA GGGAGAGTTG GCCAATTTCA GATTCCAGAA GGATTTCCTC
    AGGCCGTTCG AGCACATAAT GAAAAAGAAC AGATCTCCGA CCATTAGAGA CATGGTGGTT
    CGCTGTATTG CCCAGATGGT CAACTCTCAG GCTGGCAATA TTAGATCAGG GTGGAAGAAC
    ATTTTCTCTG TGTTTCATCT GGCTGCTTCT GATCAGGATG AGAGTATAGT TGAGCTGGCC
    TTCCAGACAA CTGGACACAT CGTCACGAAT GTATTTGAGA AGCATTTCCC AGCTACCATT
    GACTCCTTCC AGGACGCTGT CAAGTGTTTG TCTGAGTTCG CCTGCAACGC TTCTTTTCCA
    GACACCAGCA TGGAGGCTAT TCGTCTGATC CGCCACTGCG CCAAATATGT GTCAGACCGA
    CCCCAGGCGT TTAAAGACTA TACCAGTGAT GATATGAATG TTGCCCCGGA GGACCGTGTT
    TGGGTCAGAG GCTGGTTCCC CATCCTTTTT GAGCTGTCAT GCATCATCAA CAGATGCAAA
    CTGGACGTCC GGACCAGGGG TCTGACAGTG ATGTTTGAGG TGATGAAGAC GTACGGTCAT
    ACCTATGAGA AACACTGGTG GCAGGATCTG TTCCGCATCG TCTTCAGGAT CTTCGACAAC
    ATGAAGCTTC CTGAGCAACA GACCGAGAAA GCGGAGTGGA TGACCACCAC CTGCAATCAT
    GCACTTTATG CCATCTGTGA CGTTTTTACA CAGTACTTTG AGTCTCTCAG TGATGTTCTT
    TTAGATGATA TCTTATCTCA GCTCTACTGG TGTGTCCAGC AAGATAATGA GCAATTAGCC
    AGATCAGGCA CTAACTGCCT TGAAAACGTG GTCATCCTTA ATGGTGAGAA GTTCAATCCT
    GAGACTTGGG ACAAGACATG CAACTGCATG CTGGACATCT TCAAAACCAC CATCCCACAC
    ATGCTGCTGA CTTGGAGACC GGCTGGTGCT GAAGGAGATC ACATGACTCA GCTGGAGTCT
    GATAAACAGC TGGACTCCAT TTCCCAGAAG TCAGTGGATA TTCAGACCCG CTCAGATGAC
    CAGCAGTCTG TTAACAGTAT GGAGAAGGCT TTGGCTGACA ACCGCAGATA CAGTCAGTTC
    AGTGTGGCTT CAGAAGCTCA GGAACAGAGG CTGTTTGCTG CGCTACTCAT TAAGTGTGTG
    GTTCAGCTGG AGCTCATTCA GACCATTGAC AACATCGTCT TCTTTCCTGC TACCAGTAAG
    AAAGAAGATG CGGAAAACTT TGCGGCTGCA CAGAGAGATG CTTTAGATGC TGATATACAT
    GTGGACACAC AGGACCAGGG AATGTATCGC TATCTGACCT CAGAACAGCT CTTCAAACTG
    CTCGACTGTC TGCTGGAGTC TCATCGATTT GCAAAGGCCT TCAACTCCAA TAATGAGCAA
    AGAACTGCTC TGTGGAAAGC CGGTTTCAAA GGGAAGTCGA AGCCCAATCT GCTGAAACAG
    GAGACGAGCA GCCTGGCGTG TGGTCTGCGC ATCCTCTTCC GCATGTACAC AGATCCAAGC
    CGACGGGATG CCTGGGAGGA GGTGCAGAGG AGACTGCTCA ACGTTTGCAG TGACGCAGTG
    GCTTATTTTC TGACCCTCAC GTCTGAAAGT CATCGCGAAG CCTGGACAAA CCTTCTGCTG
    CTCTTCCTGA CCAAAGTGCT GAAGATCAGT GATGATAGAT TCAAGGCTCA TGCGTCCCGC
    TACTACCCTC TGCTCTGTGA AATCATGCAG TTCGACCTGA TCCCGGAGCT GCGGGCCGTC
    CTGCGCAAGT TTTTCCTGCG CATTGGACTG GTTTTCCAGA TCACCAACAG CCTGGAAACA
    AATCAAACTG TCAGCAACGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49264
    Clone ID Related Accession (Same CDS sequence) XM_009297396.1
    Accession Version XM_009297396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGAA
    ATGAAGGCAA GCCATGAGGG TGAGGCTGAG TCTGCGGAGG TCAGTCCAAA CTCTCTGGAA
    GAGGAGGGAG GCTTGGGCAG CGACAACGAA AATGTTCACG CTAACGGCAT CCCTGGCACT
    CCCATCTCCG CAAGCTTCAC CCCATCCCTG CCCGACGACA GGCTGTCTGT CTCTTCTAAT
    GACACTCAGG AATCGGTAGC CACCTCTGGT CAAGCACCAG GCGCAAAGTT TTCTCACATT
    CTCCAGAAAG ATGCCTTCCT GGTGTTTCGT TCCCTCTGCA AACTCTCCAT GAAGCCTCTT
    TCGGATGGAC CGCCTGATCC CAAATCCCAT GAGCTGCGGT CCAAGGTCCT CTCTCTGCAG
    CTCCTCCTGT CCATTCTGCA GAACGCTGGA CCCATCTTCA AGACCAACGA GATGTTCATC
    AACGCCATCA AGCAGTACCT GTGCGTAGCG CTGTCTAAAA ACGGCGTCTC CTCCGTCCCC
    GAGGTGTTCG AGCTGTCTCT GTCCATCTTC CTCACCCTCC TGTCCAACTT CAAGACACAT
    CTCAAGATGC AAATCGAGGT GTTTTTCAAA GAGATTTTTC TGTATATTCT GGAAACTTCC
    ACAAGCTCAT ATGACCACAA GTGGATGGTC ATTCAAACCC TCACTAGAAT ATGTGCTGAT
    GCACAGAGTG TAGTAGATAT CTATGTTAAT TATGATTGTG ACCTGAATGC TGCTAATATT
    TTCGAGAGGC TGGTGAATGA CCTCTCAAAG ATTGCACAAG GACGAGGAGG TCATGAACTG
    GGTACAACCC CACTTCAGGA GCTGACTTTA AGAAAGAAAG GATTGGAGTG TCTGGTGTCC
    ATTCTCAAGT GTATGGTGGA GTGGAGCAAA GACCAATACG TCAACCCCAA TTCCCAGACA
    AGTCTTGGGA GAGCAGGCCA AGAAAAGCCT TCAGAGCAGG AGAGCAATGA GTCGAAGCAT
    CCAGAAACGA TCAACCGTTA CGGCAGCATT AACTCTTTGG ACTCCACTGC ATCATCAGGC
    ATTGGGAGCT ACAGCACACA GATGTCCGGA ACAGACAACC CGGAGCAGTT TGAGGTCCTC
    AAACAACAGA AGGAGATCAT AGAGCAAGGC ATTGACCTGT TCAACAAAAA ACCAAAGAGG
    GGCATTCAGT ACCTGCAGGA GCAAGGCATG CTGGGAACAA CGCCTGAAGA TATTGCCCAG
    TTTTTACATC AAGAGGAGAG ACTTGACTCT ATCCAGGTAG GAGAGTTTCT AGGAGACAAC
    GACCGCATCA ATAAAGAGGT CATGTATGCC TACGTCGACC AAATGGACTT CCAGGGCAAA
    GACTTCGTGC CAGCGCTGCG CATGTTCCTG GAGGGTTTCC GTCTGCCCGG CGAAGCCCAG
    AAAATTGATC GACTGATGGA GAAATTTGCT GCTAGATATT TAGAATGCAA CCAAGGGCAA
    ACACTGTTCG CCAGCGCTGA CACCGCATAC GTTCTTGCGT ATTCAATCAT CATGTTGACT
    ACCGATCTGC ACAGTCCACA GGTGAAGAAT AAAATGACGA AAGAGCAATA CATCAAGATG
    AATCGAGGTA TCAATGACAG TAAAGATTTA CCGGAGGAGT ATCTCTCAGC CATTTATGAT
    GAAATCGCAG GGAAGAAAAT CTCCATGAAG GAGACAAAGG AGTTAACTCT CAAATCAAAT
    AAGCAAAGTG TGGCGAGTGA GAAGCAGAGG AGGCTGCTGT ATAATGTTGA GATGGAGCAG
    ATGGCAAAAA CAGCAAAGGC TCTGATGGAG GCCGTGAGCC ACGTTCAAGC CCCCTTCACC
    AGCGCCACAC ATCTTGAGCA TGTCAGACCA ATGTTTAAGC TGGCCTGGAC GCCCTTTCTG
    GCTGCTTTCA GTGTGGGTCT GCAGGACTGT GATGACACTG AAGTGGCCTC TCTGTGTCTG
    GAGGGAATCC GCTGTGCCAT CAGGATAGCA TGCATCTTCA GCATTCAGTT GGAGCGAGAC
    GCATATGTAC AGGCTCTGGC CCGTTTCACA CTCTTGACGG CCAGTTCTGG CATCACTGAA
    ATGAAGCAGA AGAACATCGA CACCATCAAG ACCCTCATCA CAGTGGCTCA CACTGATGGA
    AATTATCTAG GAAACTCCTG GCATGAGATC ATGAAGTGCA TTAGTCAGCT GGAGTTGGCC
    CAGCTCATCG GGACAGGAGT GAAGGCTCGG TACATATCAG GAACTGTTCG GGGAAAGGAA
    GGCTTTATCA CCAGCACCAA GGAACAAACG TCTGATGAAT ACCTGGGATT GGGAGGGAAT
    GTTGATCGCA AACAGATTGC AAGCATTCAG GAGTCTATCG GGGAGACCAG CTCGCAAAGT
    GTAGTTGTTG CTGTAGACCG GATATTCACG GGCTCTACCA GACTTGATGG AAATGCCATT
    GTGGACTTTG TGCGATGGCT GTGCGCTGTC TCGATGGATG AACTGGCCTC CCCAACACAC
    CCTCGAATGT TCAGTCTGCA GAAGATCGTG GAGATTTCAT ACTACAATAT GGGTCGTATC
    AGGTTGCAGT GGTCCAGGAT TTGGGAGGTT ATAGGGGATC ATTTCAATAA GGTTGGCTGT
    AATCCCAACG AAGATGTTGC TATTTTTGCT GTGGATTCTC TGAGGCAGTT GTCCATGAAG
    TTTTTGGAAA AGGGAGAGTT GGCCAATTTC AGATTCCAGA AGGATTTCCT CAGGCCGTTC
    GAGCACATAA TGAAAAAGAA CAGATCTCCG ACCATTAGAG ACATGGTGGT TCGCTGTATT
    GCCCAGATGG TCAACTCTCA GGCTGGCAAT ATTAGATCAG GGTGGAAGAA CATTTTCTCT
    GTGTTTCATC TGGCTGCTTC TGATCAGGAT GAGAGTATAG TTGAGCTGGC CTTCCAGACA
    ACTGGACACA TCGTCACGAA TGTATTTGAG AAGCATTTCC CAGCTACCAT TGACTCCTTC
    CAGGACGCTG TCAAGTGTTT GTCTGAGTTC GCCTGCAACG CTTCTTTTCC AGACACCAGC
    ATGGAGGCTA TTCGTCTGAT CCGCCACTGC GCCAAATATG TGTCAGACCG ACCCCAGGCG
    TTTAAAGACT ATACCAGTGA TGATATGAAT GTTGCCCCGG AGGACCGTGT TTGGGTCAGA
    GGCTGGTTCC CCATCCTTTT TGAGCTGTCA TGCATCATCA ACAGATGCAA ACTGGACGTC
    CGGACCAGGG GTCTGACAGT GATGTTTGAG GTGATGAAGA CGTACGGTCA TACCTATGAG
    AAACACTGGT GGCAGGATCT GTTCCGCATC GTCTTCAGGA TCTTCGACAA CATGAAGCTT
    CCTGAGCAAC AGACCGAGAA AGCGGAGTGG ATGACCACCA CCTGCAATCA TGCACTTTAT
    GCCATCTGTG ACGTTTTTAC ACAGTACTTT GAGTCTCTCA GTGATGTTCT TTTAGATGAT
    ATCTTATCTC AGCTCTACTG GTGTGTCCAG CAAGATAATG AGCAATTAGC CAGATCAGGC
    ACTAACTGCC TTGAAAACGT GGTCATCCTT AATGGTGAGA AGTTCAATCC TGAGACTTGG
    GACAAGACAT GCAACTGCAT GCTGGACATC TTCAAAACCA CCATCCCACA CATGCTGCTG
    ACTTGGAGAC CGGCTGGTGC TGAAGGAGAT CACATGACTC AGCTGGAGTC TGATAAACAG
    CTGGACTCCA TTTCCCAGAA GTCAGTGGAT ATTCAGACCC GCTCAGATGA CCAGCAGTCT
    GTTAACAGTA TGGAGAAGGC TTTGGCTGAC AACCGCAGAT ACAGTCAGTT CAGTGTGGCT
    TCAGAAGCTC AGGAACAGAG GCTGTTTGCT GCGCTACTCA TTAAGTGTGT GGTTCAGCTG
    GAGCTCATTC AGACCATTGA CAACATCGTC TTCTTTCCTG CTACCAGTAA GAAAGAAGAT
    GCGGAAAACT TTGCGGCTGC ACAGAGAGAT GCTTTAGATG CTGATATACA TGTGGACACA
    CAGGACCAGG GAATGTATCG CTATCTGACC TCAGAACAGC TCTTCAAACT GCTCGACTGT
    CTGCTGGAGT CTCATCGATT TGCAAAGGCC TTCAACTCCA ATAATGAGCA AAGAACTGCT
    CTGTGGAAAG CCGGTTTCAA AGGGAAGTCG AAGCCCAATC TGCTGAAACA GGAGACGAGC
    AGCCTGGCGT GTGGTCTGCG CATCCTCTTC CGCATGTACA CAGATCCAAG CCGACGGGAT
    GCCTGGGAGG AGGTGCAGAG GAGACTGCTC AACGTTTGCA GTGACGCAGT GGCTTATTTT
    CTGACCCTCA CGTCTGAAAG TCATCGCGAA GCCTGGACAA ACCTTCTGCT GCTCTTCCTG
    ACCAAAGTGC TGAAGATCAG TGATGATAGA TTCAAGGCTC ATGCGTCCCG CTACTACCCT
    CTGCTCTGTG AAATCATGCA GTTCGACCTG ATCCCGGAGC TGCGGGCCGT CCTGCGCAAG
    TTTTTCCTGC GCATTGGACT GGTTTTCCAG ATCACCAACA GCCTGGAAAC AAATCAAACT
    GTCAGCAACG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295671.1
    CDS206..5800
    Translation

    Target ORF information:

    RefSeq Version XM_009297396.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT
    TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA
    GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG
    CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG
    GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT
    GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA
    AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG
    AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGAA
    ATGAAGGCAA GCCATGAGGG TGAGGCTGAG TCTGCGGAGG TCAGTCCAAA CTCTCTGGAA
    GAGGAGGGAG GCTTGGGCAG CGACAACGAA AATGTTCACG CTAACGGCAT CCCTGGCACT
    CCCATCTCCG CAAGCTTCAC CCCATCCCTG CCCGACGACA GGCTGTCTGT CTCTTCTAAT
    GACACTCAGG AATCGGTAGC CACCTCTGGT CAAGCACCAG GCGCAAAGTT TTCTCACATT
    CTCCAGAAAG ATGCCTTCCT GGTGTTTCGT TCCCTCTGCA AACTCTCCAT GAAGCCTCTT
    TCGGATGGAC CGCCTGATCC CAAATCCCAT GAGCTGCGGT CCAAGGTCCT CTCTCTGCAG
    CTCCTCCTGT CCATTCTGCA GAACGCTGGA CCCATCTTCA AGACCAACGA GATGTTCATC
    AACGCCATCA AGCAGTACCT GTGCGTAGCG CTGTCTAAAA ACGGCGTCTC CTCCGTCCCC
    GAGGTGTTCG AGCTGTCTCT GTCCATCTTC CTCACCCTCC TGTCCAACTT CAAGACACAT
    CTCAAGATGC AAATCGAGGT GTTTTTCAAA GAGATTTTTC TGTATATTCT GGAAACTTCC
    ACAAGCTCAT ATGACCACAA GTGGATGGTC ATTCAAACCC TCACTAGAAT ATGTGCTGAT
    GCACAGAGTG TAGTAGATAT CTATGTTAAT TATGATTGTG ACCTGAATGC TGCTAATATT
    TTCGAGAGGC TGGTGAATGA CCTCTCAAAG ATTGCACAAG GACGAGGAGG TCATGAACTG
    GGTACAACCC CACTTCAGGA GCTGACTTTA AGAAAGAAAG GATTGGAGTG TCTGGTGTCC
    ATTCTCAAGT GTATGGTGGA GTGGAGCAAA GACCAATACG TCAACCCCAA TTCCCAGACA
    AGTCTTGGGA GAGCAGGCCA AGAAAAGCCT TCAGAGCAGG AGAGCAATGA GTCGAAGCAT
    CCAGAAACGA TCAACCGTTA CGGCAGCATT AACTCTTTGG ACTCCACTGC ATCATCAGGC
    ATTGGGAGCT ACAGCACACA GATGTCCGGA ACAGACAACC CGGAGCAGTT TGAGGTCCTC
    AAACAACAGA AGGAGATCAT AGAGCAAGGC ATTGACCTGT TCAACAAAAA ACCAAAGAGG
    GGCATTCAGT ACCTGCAGGA GCAAGGCATG CTGGGAACAA CGCCTGAAGA TATTGCCCAG
    TTTTTACATC AAGAGGAGAG ACTTGACTCT ATCCAGGTAG GAGAGTTTCT AGGAGACAAC
    GACCGCATCA ATAAAGAGGT CATGTATGCC TACGTCGACC AAATGGACTT CCAGGGCAAA
    GACTTCGTGC CAGCGCTGCG CATGTTCCTG GAGGGTTTCC GTCTGCCCGG CGAAGCCCAG
    AAAATTGATC GACTGATGGA GAAATTTGCT GCTAGATATT TAGAATGCAA CCAAGGGCAA
    ACACTGTTCG CCAGCGCTGA CACCGCATAC GTTCTTGCGT ATTCAATCAT CATGTTGACT
    ACCGATCTGC ACAGTCCACA GGTGAAGAAT AAAATGACGA AAGAGCAATA CATCAAGATG
    AATCGAGGTA TCAATGACAG TAAAGATTTA CCGGAGGAGT ATCTCTCAGC CATTTATGAT
    GAAATCGCAG GGAAGAAAAT CTCCATGAAG GAGACAAAGG AGTTAACTCT CAAATCAAAT
    AAGCAAAGTG TGGCGAGTGA GAAGCAGAGG AGGCTGCTGT ATAATGTTGA GATGGAGCAG
    ATGGCAAAAA CAGCAAAGGC TCTGATGGAG GCCGTGAGCC ACGTTCAAGC CCCCTTCACC
    AGCGCCACAC ATCTTGAGCA TGTCAGACCA ATGTTTAAGC TGGCCTGGAC GCCCTTTCTG
    GCTGCTTTCA GTGTGGGTCT GCAGGACTGT GATGACACTG AAGTGGCCTC TCTGTGTCTG
    GAGGGAATCC GCTGTGCCAT CAGGATAGCA TGCATCTTCA GCATTCAGTT GGAGCGAGAC
    GCATATGTAC AGGCTCTGGC CCGTTTCACA CTCTTGACGG CCAGTTCTGG CATCACTGAA
    ATGAAGCAGA AGAACATCGA CACCATCAAG ACCCTCATCA CAGTGGCTCA CACTGATGGA
    AATTATCTAG GAAACTCCTG GCATGAGATC ATGAAGTGCA TTAGTCAGCT GGAGTTGGCC
    CAGCTCATCG GGACAGGAGT GAAGGCTCGG TACATATCAG GAACTGTTCG GGGAAAGGAA
    GGCTTTATCA CCAGCACCAA GGAACAAACG TCTGATGAAT ACCTGGGATT GGGAGGGAAT
    GTTGATCGCA AACAGATTGC AAGCATTCAG GAGTCTATCG GGGAGACCAG CTCGCAAAGT
    GTAGTTGTTG CTGTAGACCG GATATTCACG GGCTCTACCA GACTTGATGG AAATGCCATT
    GTGGACTTTG TGCGATGGCT GTGCGCTGTC TCGATGGATG AACTGGCCTC CCCAACACAC
    CCTCGAATGT TCAGTCTGCA GAAGATCGTG GAGATTTCAT ACTACAATAT GGGTCGTATC
    AGGTTGCAGT GGTCCAGGAT TTGGGAGGTT ATAGGGGATC ATTTCAATAA GGTTGGCTGT
    AATCCCAACG AAGATGTTGC TATTTTTGCT GTGGATTCTC TGAGGCAGTT GTCCATGAAG
    TTTTTGGAAA AGGGAGAGTT GGCCAATTTC AGATTCCAGA AGGATTTCCT CAGGCCGTTC
    GAGCACATAA TGAAAAAGAA CAGATCTCCG ACCATTAGAG ACATGGTGGT TCGCTGTATT
    GCCCAGATGG TCAACTCTCA GGCTGGCAAT ATTAGATCAG GGTGGAAGAA CATTTTCTCT
    GTGTTTCATC TGGCTGCTTC TGATCAGGAT GAGAGTATAG TTGAGCTGGC CTTCCAGACA
    ACTGGACACA TCGTCACGAA TGTATTTGAG AAGCATTTCC CAGCTACCAT TGACTCCTTC
    CAGGACGCTG TCAAGTGTTT GTCTGAGTTC GCCTGCAACG CTTCTTTTCC AGACACCAGC
    ATGGAGGCTA TTCGTCTGAT CCGCCACTGC GCCAAATATG TGTCAGACCG ACCCCAGGCG
    TTTAAAGACT ATACCAGTGA TGATATGAAT GTTGCCCCGG AGGACCGTGT TTGGGTCAGA
    GGCTGGTTCC CCATCCTTTT TGAGCTGTCA TGCATCATCA ACAGATGCAA ACTGGACGTC
    CGGACCAGGG GTCTGACAGT GATGTTTGAG GTGATGAAGA CGTACGGTCA TACCTATGAG
    AAACACTGGT GGCAGGATCT GTTCCGCATC GTCTTCAGGA TCTTCGACAA CATGAAGCTT
    CCTGAGCAAC AGACCGAGAA AGCGGAGTGG ATGACCACCA CCTGCAATCA TGCACTTTAT
    GCCATCTGTG ACGTTTTTAC ACAGTACTTT GAGTCTCTCA GTGATGTTCT TTTAGATGAT
    ATCTTATCTC AGCTCTACTG GTGTGTCCAG CAAGATAATG AGCAATTAGC CAGATCAGGC
    ACTAACTGCC TTGAAAACGT GGTCATCCTT AATGGTGAGA AGTTCAATCC TGAGACTTGG
    GACAAGACAT GCAACTGCAT GCTGGACATC TTCAAAACCA CCATCCCACA CATGCTGCTG
    ACTTGGAGAC CGGCTGGTGC TGAAGGAGAT CACATGACTC AGCTGGAGTC TGATAAACAG
    CTGGACTCCA TTTCCCAGAA GTCAGTGGAT ATTCAGACCC GCTCAGATGA CCAGCAGTCT
    GTTAACAGTA TGGAGAAGGC TTTGGCTGAC AACCGCAGAT ACAGTCAGTT CAGTGTGGCT
    TCAGAAGCTC AGGAACAGAG GCTGTTTGCT GCGCTACTCA TTAAGTGTGT GGTTCAGCTG
    GAGCTCATTC AGACCATTGA CAACATCGTC TTCTTTCCTG CTACCAGTAA GAAAGAAGAT
    GCGGAAAACT TTGCGGCTGC ACAGAGAGAT GCTTTAGATG CTGATATACA TGTGGACACA
    CAGGACCAGG GAATGTATCG CTATCTGACC TCAGAACAGC TCTTCAAACT GCTCGACTGT
    CTGCTGGAGT CTCATCGATT TGCAAAGGCC TTCAACTCCA ATAATGAGCA AAGAACTGCT
    CTGTGGAAAG CCGGTTTCAA AGGGAAGTCG AAGCCCAATC TGCTGAAACA GGAGACGAGC
    AGCCTGGCGT GTGGTCTGCG CATCCTCTTC CGCATGTACA CAGATCCAAG CCGACGGGAT
    GCCTGGGAGG AGGTGCAGAG GAGACTGCTC AACGTTTGCA GTGACGCAGT GGCTTATTTT
    CTGACCCTCA CGTCTGAAAG TCATCGCGAA GCCTGGACAA ACCTTCTGCT GCTCTTCCTG
    ACCAAAGTGC TGAAGATCAG TGATGATAGA TTCAAGGCTC ATGCGTCCCG CTACTACCCT
    CTGCTCTGTG AAATCATGCA GTTCGACCTG ATCCCGGAGC TGCGGGCCGT CCTGCGCAAG
    TTTTTCCTGC GCATTGGACT GGTTTTCCAG ATCACCAACA GCCTGGAAAC AAATCAAACT
    GTCAGCAACG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49265
    Clone ID Related Accession (Same CDS sequence) XM_009297397.1
    Accession Version XM_009297397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG
    GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT
    CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC
    GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA
    CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC
    TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG
    CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC
    TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT
    AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC
    CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT
    TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA
    ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT
    TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA
    CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG
    AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA
    TACGTCAACC CCAATTCCCA GACAAGTCTT GGGAGAGCAG GCCAAGAAAA GCCTTCAGAG
    CAGGAGAGCA ATGAGTCGAA GCATCCAGAA ACGATCAACC GTTACGGCAG CATTAACTCT
    TTGGACTCCA CTGCATCATC AGGCATTGGG AGCTACAGCA CACAGATGTC CGGAACAGAC
    AACCCGGAGC AGTTTGAGGT CCTCAAACAA CAGAAGGAGA TCATAGAGCA AGGCATTGAC
    CTGTTCAACA AAAAACCAAA GAGGGGCATT CAGTACCTGC AGGAGCAAGG CATGCTGGGA
    ACAACGCCTG AAGATATTGC CCAGTTTTTA CATCAAGAGG AGAGACTTGA CTCTATCCAG
    GTAGGAGAGT TTCTAGGAGA CAACGACCGC ATCAATAAAG AGGTCATGTA TGCCTACGTC
    GACCAAATGG ACTTCCAGGG CAAAGACTTC GTGCCAGCGC TGCGCATGTT CCTGGAGGGT
    TTCCGTCTGC CCGGCGAAGC CCAGAAAATT GATCGACTGA TGGAGAAATT TGCTGCTAGA
    TATTTAGAAT GCAACCAAGG GCAAACACTG TTCGCCAGCG CTGACACCGC ATACGTTCTT
    GCGTATTCAA TCATCATGTT GACTACCGAT CTGCACAGTC CACAGGTGAA GAATAAAATG
    ACGAAAGAGC AATACATCAA GATGAATCGA GGTATCAATG ACAGTAAAGA TTTACCGGAG
    GAGTATCTCT CAGCCATTTA TGATGAAATC GCAGGGAAGA AAATCTCCAT GAAGGAGACA
    AAGGAGTTAA CTCTCAAATC AAATAAGCAA AGTGTGGCGA GTGAGAAGCA GAGGAGGCTG
    CTGTATAATG TTGAGATGGA GCAGATGGCA AAAACAGCAA AGGCTCTGAT GGAGGCCGTG
    AGCCACGTTC AAGCCCCCTT CACCAGCGCC ACACATCTTG AGCATGTCAG ACCAATGTTT
    AAGCTGGCCT GGACGCCCTT TCTGGCTGCT TTCAGTGTGG GTCTGCAGGA CTGTGATGAC
    ACTGAAGTGG CCTCTCTGTG TCTGGAGGGA ATCCGCTGTG CCATCAGGAT AGCATGCATC
    TTCAGCATTC AGTTGGAGCG AGACGCATAT GTACAGGCTC TGGCCCGTTT CACACTCTTG
    ACGGCCAGTT CTGGCATCAC TGAAATGAAG CAGAAGAACA TCGACACCAT CAAGACCCTC
    ATCACAGTGG CTCACACTGA TGGAAATTAT CTAGGAAACT CCTGGCATGA GATCATGAAG
    TGCATTAGTC AGCTGGAGTT GGCCCAGCTC ATCGGGACAG GAGTGAAGGC TCGGTACATA
    TCAGGAACTG TTCGGGGAAA GGAAGGCTTT ATCACCAGCA CCAAGGAACA AACGTCTGAT
    GAATACCTGG GATTGGGAGG GAATGTTGAT CGCAAACAGA TTGCAAGCAT TCAGGAGTCT
    ATCGGGGAGA CCAGCTCGCA AAGTGTAGTT GTTGCTGTAG ACCGGATATT CACGGGCTCT
    ACCAGACTTG ATGGAAATGC CATTGTGGAC TTTGTGCGAT GGCTGTGCGC TGTCTCGATG
    GATGAACTGG CCTCCCCAAC ACACCCTCGA ATGTTCAGTC TGCAGAAGAT CGTGGAGATT
    TCATACTACA ATATGGGTCG TATCAGGTTG CAGTGGTCCA GGATTTGGGA GGTTATAGGG
    GATCATTTCA ATAAGGTTGG CTGTAATCCC AACGAAGATG TTGCTATTTT TGCTGTGGAT
    TCTCTGAGGC AGTTGTCCAT GAAGTTTTTG GAAAAGGGAG AGTTGGCCAA TTTCAGATTC
    CAGAAGGATT TCCTCAGGCC GTTCGAGCAC ATAATGAAAA AGAACAGATC TCCGACCATT
    AGAGACATGG TGGTTCGCTG TATTGCCCAG ATGGTCAACT CTCAGGCTGG CAATATTAGA
    TCAGGGTGGA AGAACATTTT CTCTGTGTTT CATCTGGCTG CTTCTGATCA GGATGAGAGT
    ATAGTTGAGC TGGCCTTCCA GACAACTGGA CACATCGTCA CGAATGTATT TGAGAAGCAT
    TTCCCAGCTA CCATTGACTC CTTCCAGGAC GCTGTCAAGT GTTTGTCTGA GTTCGCCTGC
    AACGCTTCTT TTCCAGACAC CAGCATGGAG GCTATTCGTC TGATCCGCCA CTGCGCCAAA
    TATGTGTCAG ACCGACCCCA GGCGTTTAAA GACTATACCA GTGATGATAT GAATGTTGCC
    CCGGAGGACC GTGTTTGGGT CAGAGGCTGG TTCCCCATCC TTTTTGAGCT GTCATGCATC
    ATCAACAGAT GCAAACTGGA CGTCCGGACC AGGGGTCTGA CAGTGATGTT TGAGGTGATG
    AAGACGTACG GTCATACCTA TGAGAAACAC TGGTGGCAGG ATCTGTTCCG CATCGTCTTC
    AGGATCTTCG ACAACATGAA GCTTCCTGAG CAACAGACCG AGAAAGCGGA GTGGATGACC
    ACCACCTGCA ATCATGCACT TTATGCCATC TGTGACGTTT TTACACAGTA CTTTGAGTCT
    CTCAGTGATG TTCTTTTAGA TGATATCTTA TCTCAGCTCT ACTGGTGTGT CCAGCAAGAT
    AATGAGCAAT TAGCCAGATC AGGCACTAAC TGCCTTGAAA ACGTGGTCAT CCTTAATGGT
    GAGAAGTTCA ATCCTGAGAC TTGGGACAAG ACATGCAACT GCATGCTGGA CATCTTCAAA
    ACCACCATCC CACACATGCT GCTGACTTGG AGACCGGCTG GTGCTGAAGG AGATCACATG
    ACTCAGCTGG AGTCTGATAA ACAGCTGGAC TCCATTTCCC AGAAGTCAGT GGATATTCAG
    ACCCGCTCAG ATGACCAGCA GTCTGTTAAC AGTATGGAGA AGGCTTTGGC TGACAACCGC
    AGATACAGTC AGTTCAGTGT GGCTTCAGAA GCTCAGGAAC AGAGGCTGTT TGCTGCGCTA
    CTCATTAAGT GTGTGGTTCA GCTGGAGCTC ATTCAGACCA TTGACAACAT CGTCTTCTTT
    CCTGCTACCA GTAAGAAAGA AGATGCGGAA AACTTTGCGG CTGCACAGAG AGATGCTTTA
    GATGCTGATA TACATGTGGA CACACAGGAC CAGGGAATGT ATCGCTATCT GACCTCAGAA
    CAGCTCTTCA AACTGCTCGA CTGTCTGCTG GAGTCTCATC GATTTGCAAA GGCCTTCAAC
    TCCAATAATG AGCAAAGAAC TGCTCTGTGG AAAGCCGGTT TCAAAGGGAA GTCGAAGCCC
    AATCTGCTGA AACAGGAGAC GAGCAGCCTG GCGTGTGGTC TGCGCATCCT CTTCCGCATG
    TACACAGATC CAAGCCGACG GGATGCCTGG GAGGAGGTGC AGAGGAGACT GCTCAACGTT
    TGCAGTGACG CAGTGGCTTA TTTTCTGACC CTCACGTCTG AAAGTCATCG CGAAGCCTGG
    ACAAACCTTC TGCTGCTCTT CCTGACCAAA GTGCTGAAGA TCAGTGATGA TAGATTCAAG
    GCTCATGCGT CCCGCTACTA CCCTCTGCTC TGTGAAATCA TGCAGTTCGA CCTGATCCCG
    GAGCTGCGGG CCGTCCTGCG CAAGTTTTTC CTGCGCATTG GACTGGTTTT CCAGATCACC
    AACAGCCTGG AAACAAATCA AACTGTCAGC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295672.1
    CDS206..5764
    Translation

    Target ORF information:

    RefSeq Version XM_009297397.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG
    GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT
    CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC
    GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA
    CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC
    TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG
    CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC
    TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT
    AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC
    CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT
    TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA
    ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT
    TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA
    CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG
    AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA
    TACGTCAACC CCAATTCCCA GACAAGTCTT GGGAGAGCAG GCCAAGAAAA GCCTTCAGAG
    CAGGAGAGCA ATGAGTCGAA GCATCCAGAA ACGATCAACC GTTACGGCAG CATTAACTCT
    TTGGACTCCA CTGCATCATC AGGCATTGGG AGCTACAGCA CACAGATGTC CGGAACAGAC
    AACCCGGAGC AGTTTGAGGT CCTCAAACAA CAGAAGGAGA TCATAGAGCA AGGCATTGAC
    CTGTTCAACA AAAAACCAAA GAGGGGCATT CAGTACCTGC AGGAGCAAGG CATGCTGGGA
    ACAACGCCTG AAGATATTGC CCAGTTTTTA CATCAAGAGG AGAGACTTGA CTCTATCCAG
    GTAGGAGAGT TTCTAGGAGA CAACGACCGC ATCAATAAAG AGGTCATGTA TGCCTACGTC
    GACCAAATGG ACTTCCAGGG CAAAGACTTC GTGCCAGCGC TGCGCATGTT CCTGGAGGGT
    TTCCGTCTGC CCGGCGAAGC CCAGAAAATT GATCGACTGA TGGAGAAATT TGCTGCTAGA
    TATTTAGAAT GCAACCAAGG GCAAACACTG TTCGCCAGCG CTGACACCGC ATACGTTCTT
    GCGTATTCAA TCATCATGTT GACTACCGAT CTGCACAGTC CACAGGTGAA GAATAAAATG
    ACGAAAGAGC AATACATCAA GATGAATCGA GGTATCAATG ACAGTAAAGA TTTACCGGAG
    GAGTATCTCT CAGCCATTTA TGATGAAATC GCAGGGAAGA AAATCTCCAT GAAGGAGACA
    AAGGAGTTAA CTCTCAAATC AAATAAGCAA AGTGTGGCGA GTGAGAAGCA GAGGAGGCTG
    CTGTATAATG TTGAGATGGA GCAGATGGCA AAAACAGCAA AGGCTCTGAT GGAGGCCGTG
    AGCCACGTTC AAGCCCCCTT CACCAGCGCC ACACATCTTG AGCATGTCAG ACCAATGTTT
    AAGCTGGCCT GGACGCCCTT TCTGGCTGCT TTCAGTGTGG GTCTGCAGGA CTGTGATGAC
    ACTGAAGTGG CCTCTCTGTG TCTGGAGGGA ATCCGCTGTG CCATCAGGAT AGCATGCATC
    TTCAGCATTC AGTTGGAGCG AGACGCATAT GTACAGGCTC TGGCCCGTTT CACACTCTTG
    ACGGCCAGTT CTGGCATCAC TGAAATGAAG CAGAAGAACA TCGACACCAT CAAGACCCTC
    ATCACAGTGG CTCACACTGA TGGAAATTAT CTAGGAAACT CCTGGCATGA GATCATGAAG
    TGCATTAGTC AGCTGGAGTT GGCCCAGCTC ATCGGGACAG GAGTGAAGGC TCGGTACATA
    TCAGGAACTG TTCGGGGAAA GGAAGGCTTT ATCACCAGCA CCAAGGAACA AACGTCTGAT
    GAATACCTGG GATTGGGAGG GAATGTTGAT CGCAAACAGA TTGCAAGCAT TCAGGAGTCT
    ATCGGGGAGA CCAGCTCGCA AAGTGTAGTT GTTGCTGTAG ACCGGATATT CACGGGCTCT
    ACCAGACTTG ATGGAAATGC CATTGTGGAC TTTGTGCGAT GGCTGTGCGC TGTCTCGATG
    GATGAACTGG CCTCCCCAAC ACACCCTCGA ATGTTCAGTC TGCAGAAGAT CGTGGAGATT
    TCATACTACA ATATGGGTCG TATCAGGTTG CAGTGGTCCA GGATTTGGGA GGTTATAGGG
    GATCATTTCA ATAAGGTTGG CTGTAATCCC AACGAAGATG TTGCTATTTT TGCTGTGGAT
    TCTCTGAGGC AGTTGTCCAT GAAGTTTTTG GAAAAGGGAG AGTTGGCCAA TTTCAGATTC
    CAGAAGGATT TCCTCAGGCC GTTCGAGCAC ATAATGAAAA AGAACAGATC TCCGACCATT
    AGAGACATGG TGGTTCGCTG TATTGCCCAG ATGGTCAACT CTCAGGCTGG CAATATTAGA
    TCAGGGTGGA AGAACATTTT CTCTGTGTTT CATCTGGCTG CTTCTGATCA GGATGAGAGT
    ATAGTTGAGC TGGCCTTCCA GACAACTGGA CACATCGTCA CGAATGTATT TGAGAAGCAT
    TTCCCAGCTA CCATTGACTC CTTCCAGGAC GCTGTCAAGT GTTTGTCTGA GTTCGCCTGC
    AACGCTTCTT TTCCAGACAC CAGCATGGAG GCTATTCGTC TGATCCGCCA CTGCGCCAAA
    TATGTGTCAG ACCGACCCCA GGCGTTTAAA GACTATACCA GTGATGATAT GAATGTTGCC
    CCGGAGGACC GTGTTTGGGT CAGAGGCTGG TTCCCCATCC TTTTTGAGCT GTCATGCATC
    ATCAACAGAT GCAAACTGGA CGTCCGGACC AGGGGTCTGA CAGTGATGTT TGAGGTGATG
    AAGACGTACG GTCATACCTA TGAGAAACAC TGGTGGCAGG ATCTGTTCCG CATCGTCTTC
    AGGATCTTCG ACAACATGAA GCTTCCTGAG CAACAGACCG AGAAAGCGGA GTGGATGACC
    ACCACCTGCA ATCATGCACT TTATGCCATC TGTGACGTTT TTACACAGTA CTTTGAGTCT
    CTCAGTGATG TTCTTTTAGA TGATATCTTA TCTCAGCTCT ACTGGTGTGT CCAGCAAGAT
    AATGAGCAAT TAGCCAGATC AGGCACTAAC TGCCTTGAAA ACGTGGTCAT CCTTAATGGT
    GAGAAGTTCA ATCCTGAGAC TTGGGACAAG ACATGCAACT GCATGCTGGA CATCTTCAAA
    ACCACCATCC CACACATGCT GCTGACTTGG AGACCGGCTG GTGCTGAAGG AGATCACATG
    ACTCAGCTGG AGTCTGATAA ACAGCTGGAC TCCATTTCCC AGAAGTCAGT GGATATTCAG
    ACCCGCTCAG ATGACCAGCA GTCTGTTAAC AGTATGGAGA AGGCTTTGGC TGACAACCGC
    AGATACAGTC AGTTCAGTGT GGCTTCAGAA GCTCAGGAAC AGAGGCTGTT TGCTGCGCTA
    CTCATTAAGT GTGTGGTTCA GCTGGAGCTC ATTCAGACCA TTGACAACAT CGTCTTCTTT
    CCTGCTACCA GTAAGAAAGA AGATGCGGAA AACTTTGCGG CTGCACAGAG AGATGCTTTA
    GATGCTGATA TACATGTGGA CACACAGGAC CAGGGAATGT ATCGCTATCT GACCTCAGAA
    CAGCTCTTCA AACTGCTCGA CTGTCTGCTG GAGTCTCATC GATTTGCAAA GGCCTTCAAC
    TCCAATAATG AGCAAAGAAC TGCTCTGTGG AAAGCCGGTT TCAAAGGGAA GTCGAAGCCC
    AATCTGCTGA AACAGGAGAC GAGCAGCCTG GCGTGTGGTC TGCGCATCCT CTTCCGCATG
    TACACAGATC CAAGCCGACG GGATGCCTGG GAGGAGGTGC AGAGGAGACT GCTCAACGTT
    TGCAGTGACG CAGTGGCTTA TTTTCTGACC CTCACGTCTG AAAGTCATCG CGAAGCCTGG
    ACAAACCTTC TGCTGCTCTT CCTGACCAAA GTGCTGAAGA TCAGTGATGA TAGATTCAAG
    GCTCATGCGT CCCGCTACTA CCCTCTGCTC TGTGAAATCA TGCAGTTCGA CCTGATCCCG
    GAGCTGCGGG CCGTCCTGCG CAAGTTTTTC CTGCGCATTG GACTGGTTTT CCAGATCACC
    AACAGCCTGG AAACAAATCA AACTGTCAGC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49266
    Clone ID Related Accession (Same CDS sequence) XM_009297398.1
    Accession Version XM_009297398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGAAATGA AGGCAAGCCA TGAGGGTGAG GCTGAGTCTG CGGAGGTCAG TCCAAACTCT
    CTGGAAGAGG AGGGAGGCTT GGGCAGCGAC AACGAAAATG TTCACGCTAA CGGCATCCCT
    GGCACTCCCA TCTCCGCAAG CTTCACCCCA TCCCTGCCCG ACGACAGGCT GTCTGTCTCT
    TCTAATGACA CTCAGGAATC GGTAGCCACC TCTGGTCAAG CACCAGGCGC AAAGTTTTCT
    CACATTCTCC AGAAAGATGC CTTCCTGGTG TTTCGTTCCC TCTGCAAACT CTCCATGAAG
    CCTCTTTCGG ATGGACCGCC TGATCCCAAA TCCCATGAGC TGCGGTCCAA GGTCCTCTCT
    CTGCAGCTCC TCCTGTCCAT TCTGCAGAAC GCTGGACCCA TCTTCAAGAC CAACGAGATG
    TTCATCAACG CCATCAAGCA GTACCTGTGC GTAGCGCTGT CTAAAAACGG CGTCTCCTCC
    GTCCCCGAGG TGTTCGAGCT GTCTCTGTCC ATCTTCCTCA CCCTCCTGTC CAACTTCAAG
    ACACATCTCA AGATGCAAAT CGAGGTGTTT TTCAAAGAGA TTTTTCTGTA TATTCTGGAA
    ACTTCCACAA GCTCATATGA CCACAAGTGG ATGGTCATTC AAACCCTCAC TAGAATATGT
    GCTGATGCAC AGAGTGTAGT AGATATCTAT GTTAATTATG ATTGTGACCT GAATGCTGCT
    AATATTTTCG AGAGGCTGGT GAATGACCTC TCAAAGATTG CACAAGGACG AGGAGGTCAT
    GAACTGGGTA CAACCCCACT TCAGGAGCTG ACTTTAAGAA AGAAAGGATT GGAGTGTCTG
    GTGTCCATTC TCAAGTGTAT GGTGGAGTGG AGCAAAGACC AATACGTCAA CCCCAATTCC
    CAGACAAGTC TTGGGAGAGC AGGCCAAGAA AAGCCTTCAG AGCAGGAGAG CAATGAGTCG
    AAGCATCCAG AAACGATCAA CCGTTACGGC AGCATTAACT CTTTGGACTC CACTGCATCA
    TCAGGCATTG GGAGCTACAG CACACAGATG TCCGGAACAG ACAACCCGGA GCAGTTTGAG
    GTCCTCAAAC AACAGAAGGA GATCATAGAG CAAGGCATTG ACCTGTTCAA CAAAAAACCA
    AAGAGGGGCA TTCAGTACCT GCAGGAGCAA GGCATGCTGG GAACAACGCC TGAAGATATT
    GCCCAGTTTT TACATCAAGA GGAGAGACTT GACTCTATCC AGGTAGGAGA GTTTCTAGGA
    GACAACGACC GCATCAATAA AGAGGTCATG TATGCCTACG TCGACCAAAT GGACTTCCAG
    GGCAAAGACT TCGTGCCAGC GCTGCGCATG TTCCTGGAGG GTTTCCGTCT GCCCGGCGAA
    GCCCAGAAAA TTGATCGACT GATGGAGAAA TTTGCTGCTA GATATTTAGA ATGCAACCAA
    GGGCAAACAC TGTTCGCCAG CGCTGACACC GCATACGTTC TTGCGTATTC AATCATCATG
    TTGACTACCG ATCTGCACAG TCCACAGGTG AAGAATAAAA TGACGAAAGA GCAATACATC
    AAGATGAATC GAGGTATCAA TGACAGTAAA GATTTACCGG AGGAGTATCT CTCAGCCATT
    TATGATGAAA TCGCAGGGAA GAAAATCTCC ATGAAGGAGA CAAAGGAGTT AACTCTCAAA
    TCAAATAAGC AAAGTGTGGC GAGTGAGAAG CAGAGGAGGC TGCTGTATAA TGTTGAGATG
    GAGCAGATGG CAAAAACAGC AAAGGCTCTG ATGGAGGCCG TGAGCCACGT TCAAGCCCCC
    TTCACCAGCG CCACACATCT TGAGCATGTC AGACCAATGT TTAAGCTGGC CTGGACGCCC
    TTTCTGGCTG CTTTCAGTGT GGGTCTGCAG GACTGTGATG ACACTGAAGT GGCCTCTCTG
    TGTCTGGAGG GAATCCGCTG TGCCATCAGG ATAGCATGCA TCTTCAGCAT TCAGTTGGAG
    CGAGACGCAT ATGTACAGGC TCTGGCCCGT TTCACACTCT TGACGGCCAG TTCTGGCATC
    ACTGAAATGA AGCAGAAGAA CATCGACACC ATCAAGACCC TCATCACAGT GGCTCACACT
    GATGGAAATT ATCTAGGAAA CTCCTGGCAT GAGATCATGA AGTGCATTAG TCAGCTGGAG
    TTGGCCCAGC TCATCGGGAC AGGAGTGAAG GCTCGGTACA TATCAGGAAC TGTTCGGGGA
    AAGGAAGGCT TTATCACCAG CACCAAGGAA CAAACGTCTG ATGAATACCT GGGATTGGGA
    GGGAATGTTG ATCGCAAACA GATTGCAAGC ATTCAGGAGT CTATCGGGGA GACCAGCTCG
    CAAAGTGTAG TTGTTGCTGT AGACCGGATA TTCACGGGCT CTACCAGACT TGATGGAAAT
    GCCATTGTGG ACTTTGTGCG ATGGCTGTGC GCTGTCTCGA TGGATGAACT GGCCTCCCCA
    ACACACCCTC GAATGTTCAG TCTGCAGAAG ATCGTGGAGA TTTCATACTA CAATATGGGT
    CGTATCAGGT TGCAGTGGTC CAGGATTTGG GAGGTTATAG GGGATCATTT CAATAAGGTT
    GGCTGTAATC CCAACGAAGA TGTTGCTATT TTTGCTGTGG ATTCTCTGAG GCAGTTGTCC
    ATGAAGTTTT TGGAAAAGGG AGAGTTGGCC AATTTCAGAT TCCAGAAGGA TTTCCTCAGG
    CCGTTCGAGC ACATAATGAA AAAGAACAGA TCTCCGACCA TTAGAGACAT GGTGGTTCGC
    TGTATTGCCC AGATGGTCAA CTCTCAGGCT GGCAATATTA GATCAGGGTG GAAGAACATT
    TTCTCTGTGT TTCATCTGGC TGCTTCTGAT CAGGATGAGA GTATAGTTGA GCTGGCCTTC
    CAGACAACTG GACACATCGT CACGAATGTA TTTGAGAAGC ATTTCCCAGC TACCATTGAC
    TCCTTCCAGG ACGCTGTCAA GTGTTTGTCT GAGTTCGCCT GCAACGCTTC TTTTCCAGAC
    ACCAGCATGG AGGCTATTCG TCTGATCCGC CACTGCGCCA AATATGTGTC AGACCGACCC
    CAGGCGTTTA AAGACTATAC CAGTGATGAT ATGAATGTTG CCCCGGAGGA CCGTGTTTGG
    GTCAGAGGCT GGTTCCCCAT CCTTTTTGAG CTGTCATGCA TCATCAACAG ATGCAAACTG
    GACGTCCGGA CCAGGGGTCT GACAGTGATG TTTGAGGTGA TGAAGACGTA CGGTCATACC
    TATGAGAAAC ACTGGTGGCA GGATCTGTTC CGCATCGTCT TCAGGATCTT CGACAACATG
    AAGCTTCCTG AGCAACAGAC CGAGAAAGCG GAGTGGATGA CCACCACCTG CAATCATGCA
    CTTTATGCCA TCTGTGACGT TTTTACACAG TACTTTGAGT CTCTCAGTGA TGTTCTTTTA
    GATGATATCT TATCTCAGCT CTACTGGTGT GTCCAGCAAG ATAATGAGCA ATTAGCCAGA
    TCAGGCACTA ACTGCCTTGA AAACGTGGTC ATCCTTAATG GTGAGAAGTT CAATCCTGAG
    ACTTGGGACA AGACATGCAA CTGCATGCTG GACATCTTCA AAACCACCAT CCCACACATG
    CTGCTGACTT GGAGACCGGC TGGTGCTGAA GGAGATCACA TGACTCAGCT GGAGTCTGAT
    AAACAGCTGG ACTCCATTTC CCAGAAGTCA GTGGATATTC AGACCCGCTC AGATGACCAG
    CAGTCTGTTA ACAGTATGGA GAAGGCTTTG GCTGACAACC GCAGATACAG TCAGTTCAGT
    GTGGCTTCAG AAGCTCAGGA ACAGAGGCTG TTTGCTGCGC TACTCATTAA GTGTGTGGTT
    CAGCTGGAGC TCATTCAGAC CATTGACAAC ATCGTCTTCT TTCCTGCTAC CAGTAAGAAA
    GAAGATGCGG AAAACTTTGC GGCTGCACAG AGAGATGCTT TAGATGCTGA TATACATGTG
    GACACACAGG ACCAGGGAAT GTATCGCTAT CTGACCTCAG AACAGCTCTT CAAACTGCTC
    GACTGTCTGC TGGAGTCTCA TCGATTTGCA AAGGCCTTCA ACTCCAATAA TGAGCAAAGA
    ACTGCTCTGT GGAAAGCCGG TTTCAAAGGG AAGTCGAAGC CCAATCTGCT GAAACAGGAG
    ACGAGCAGCC TGGCGTGTGG TCTGCGCATC CTCTTCCGCA TGTACACAGA TCCAAGCCGA
    CGGGATGCCT GGGAGGAGGT GCAGAGGAGA CTGCTCAACG TTTGCAGTGA CGCAGTGGCT
    TATTTTCTGA CCCTCACGTC TGAAAGTCAT CGCGAAGCCT GGACAAACCT TCTGCTGCTC
    TTCCTGACCA AAGTGCTGAA GATCAGTGAT GATAGATTCA AGGCTCATGC GTCCCGCTAC
    TACCCTCTGC TCTGTGAAAT CATGCAGTTC GACCTGATCC CGGAGCTGCG GGCCGTCCTG
    CGCAAGTTTT TCCTGCGCAT TGGACTGGTT TTCCAGATCA CCAACAGCCT GGAAACAAAT
    CAAACTGTCA GCAACGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295673.1
    CDS206..5746
    Translation

    Target ORF information:

    RefSeq Version XM_009297398.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGAAATGA AGGCAAGCCA TGAGGGTGAG GCTGAGTCTG CGGAGGTCAG TCCAAACTCT
    CTGGAAGAGG AGGGAGGCTT GGGCAGCGAC AACGAAAATG TTCACGCTAA CGGCATCCCT
    GGCACTCCCA TCTCCGCAAG CTTCACCCCA TCCCTGCCCG ACGACAGGCT GTCTGTCTCT
    TCTAATGACA CTCAGGAATC GGTAGCCACC TCTGGTCAAG CACCAGGCGC AAAGTTTTCT
    CACATTCTCC AGAAAGATGC CTTCCTGGTG TTTCGTTCCC TCTGCAAACT CTCCATGAAG
    CCTCTTTCGG ATGGACCGCC TGATCCCAAA TCCCATGAGC TGCGGTCCAA GGTCCTCTCT
    CTGCAGCTCC TCCTGTCCAT TCTGCAGAAC GCTGGACCCA TCTTCAAGAC CAACGAGATG
    TTCATCAACG CCATCAAGCA GTACCTGTGC GTAGCGCTGT CTAAAAACGG CGTCTCCTCC
    GTCCCCGAGG TGTTCGAGCT GTCTCTGTCC ATCTTCCTCA CCCTCCTGTC CAACTTCAAG
    ACACATCTCA AGATGCAAAT CGAGGTGTTT TTCAAAGAGA TTTTTCTGTA TATTCTGGAA
    ACTTCCACAA GCTCATATGA CCACAAGTGG ATGGTCATTC AAACCCTCAC TAGAATATGT
    GCTGATGCAC AGAGTGTAGT AGATATCTAT GTTAATTATG ATTGTGACCT GAATGCTGCT
    AATATTTTCG AGAGGCTGGT GAATGACCTC TCAAAGATTG CACAAGGACG AGGAGGTCAT
    GAACTGGGTA CAACCCCACT TCAGGAGCTG ACTTTAAGAA AGAAAGGATT GGAGTGTCTG
    GTGTCCATTC TCAAGTGTAT GGTGGAGTGG AGCAAAGACC AATACGTCAA CCCCAATTCC
    CAGACAAGTC TTGGGAGAGC AGGCCAAGAA AAGCCTTCAG AGCAGGAGAG CAATGAGTCG
    AAGCATCCAG AAACGATCAA CCGTTACGGC AGCATTAACT CTTTGGACTC CACTGCATCA
    TCAGGCATTG GGAGCTACAG CACACAGATG TCCGGAACAG ACAACCCGGA GCAGTTTGAG
    GTCCTCAAAC AACAGAAGGA GATCATAGAG CAAGGCATTG ACCTGTTCAA CAAAAAACCA
    AAGAGGGGCA TTCAGTACCT GCAGGAGCAA GGCATGCTGG GAACAACGCC TGAAGATATT
    GCCCAGTTTT TACATCAAGA GGAGAGACTT GACTCTATCC AGGTAGGAGA GTTTCTAGGA
    GACAACGACC GCATCAATAA AGAGGTCATG TATGCCTACG TCGACCAAAT GGACTTCCAG
    GGCAAAGACT TCGTGCCAGC GCTGCGCATG TTCCTGGAGG GTTTCCGTCT GCCCGGCGAA
    GCCCAGAAAA TTGATCGACT GATGGAGAAA TTTGCTGCTA GATATTTAGA ATGCAACCAA
    GGGCAAACAC TGTTCGCCAG CGCTGACACC GCATACGTTC TTGCGTATTC AATCATCATG
    TTGACTACCG ATCTGCACAG TCCACAGGTG AAGAATAAAA TGACGAAAGA GCAATACATC
    AAGATGAATC GAGGTATCAA TGACAGTAAA GATTTACCGG AGGAGTATCT CTCAGCCATT
    TATGATGAAA TCGCAGGGAA GAAAATCTCC ATGAAGGAGA CAAAGGAGTT AACTCTCAAA
    TCAAATAAGC AAAGTGTGGC GAGTGAGAAG CAGAGGAGGC TGCTGTATAA TGTTGAGATG
    GAGCAGATGG CAAAAACAGC AAAGGCTCTG ATGGAGGCCG TGAGCCACGT TCAAGCCCCC
    TTCACCAGCG CCACACATCT TGAGCATGTC AGACCAATGT TTAAGCTGGC CTGGACGCCC
    TTTCTGGCTG CTTTCAGTGT GGGTCTGCAG GACTGTGATG ACACTGAAGT GGCCTCTCTG
    TGTCTGGAGG GAATCCGCTG TGCCATCAGG ATAGCATGCA TCTTCAGCAT TCAGTTGGAG
    CGAGACGCAT ATGTACAGGC TCTGGCCCGT TTCACACTCT TGACGGCCAG TTCTGGCATC
    ACTGAAATGA AGCAGAAGAA CATCGACACC ATCAAGACCC TCATCACAGT GGCTCACACT
    GATGGAAATT ATCTAGGAAA CTCCTGGCAT GAGATCATGA AGTGCATTAG TCAGCTGGAG
    TTGGCCCAGC TCATCGGGAC AGGAGTGAAG GCTCGGTACA TATCAGGAAC TGTTCGGGGA
    AAGGAAGGCT TTATCACCAG CACCAAGGAA CAAACGTCTG ATGAATACCT GGGATTGGGA
    GGGAATGTTG ATCGCAAACA GATTGCAAGC ATTCAGGAGT CTATCGGGGA GACCAGCTCG
    CAAAGTGTAG TTGTTGCTGT AGACCGGATA TTCACGGGCT CTACCAGACT TGATGGAAAT
    GCCATTGTGG ACTTTGTGCG ATGGCTGTGC GCTGTCTCGA TGGATGAACT GGCCTCCCCA
    ACACACCCTC GAATGTTCAG TCTGCAGAAG ATCGTGGAGA TTTCATACTA CAATATGGGT
    CGTATCAGGT TGCAGTGGTC CAGGATTTGG GAGGTTATAG GGGATCATTT CAATAAGGTT
    GGCTGTAATC CCAACGAAGA TGTTGCTATT TTTGCTGTGG ATTCTCTGAG GCAGTTGTCC
    ATGAAGTTTT TGGAAAAGGG AGAGTTGGCC AATTTCAGAT TCCAGAAGGA TTTCCTCAGG
    CCGTTCGAGC ACATAATGAA AAAGAACAGA TCTCCGACCA TTAGAGACAT GGTGGTTCGC
    TGTATTGCCC AGATGGTCAA CTCTCAGGCT GGCAATATTA GATCAGGGTG GAAGAACATT
    TTCTCTGTGT TTCATCTGGC TGCTTCTGAT CAGGATGAGA GTATAGTTGA GCTGGCCTTC
    CAGACAACTG GACACATCGT CACGAATGTA TTTGAGAAGC ATTTCCCAGC TACCATTGAC
    TCCTTCCAGG ACGCTGTCAA GTGTTTGTCT GAGTTCGCCT GCAACGCTTC TTTTCCAGAC
    ACCAGCATGG AGGCTATTCG TCTGATCCGC CACTGCGCCA AATATGTGTC AGACCGACCC
    CAGGCGTTTA AAGACTATAC CAGTGATGAT ATGAATGTTG CCCCGGAGGA CCGTGTTTGG
    GTCAGAGGCT GGTTCCCCAT CCTTTTTGAG CTGTCATGCA TCATCAACAG ATGCAAACTG
    GACGTCCGGA CCAGGGGTCT GACAGTGATG TTTGAGGTGA TGAAGACGTA CGGTCATACC
    TATGAGAAAC ACTGGTGGCA GGATCTGTTC CGCATCGTCT TCAGGATCTT CGACAACATG
    AAGCTTCCTG AGCAACAGAC CGAGAAAGCG GAGTGGATGA CCACCACCTG CAATCATGCA
    CTTTATGCCA TCTGTGACGT TTTTACACAG TACTTTGAGT CTCTCAGTGA TGTTCTTTTA
    GATGATATCT TATCTCAGCT CTACTGGTGT GTCCAGCAAG ATAATGAGCA ATTAGCCAGA
    TCAGGCACTA ACTGCCTTGA AAACGTGGTC ATCCTTAATG GTGAGAAGTT CAATCCTGAG
    ACTTGGGACA AGACATGCAA CTGCATGCTG GACATCTTCA AAACCACCAT CCCACACATG
    CTGCTGACTT GGAGACCGGC TGGTGCTGAA GGAGATCACA TGACTCAGCT GGAGTCTGAT
    AAACAGCTGG ACTCCATTTC CCAGAAGTCA GTGGATATTC AGACCCGCTC AGATGACCAG
    CAGTCTGTTA ACAGTATGGA GAAGGCTTTG GCTGACAACC GCAGATACAG TCAGTTCAGT
    GTGGCTTCAG AAGCTCAGGA ACAGAGGCTG TTTGCTGCGC TACTCATTAA GTGTGTGGTT
    CAGCTGGAGC TCATTCAGAC CATTGACAAC ATCGTCTTCT TTCCTGCTAC CAGTAAGAAA
    GAAGATGCGG AAAACTTTGC GGCTGCACAG AGAGATGCTT TAGATGCTGA TATACATGTG
    GACACACAGG ACCAGGGAAT GTATCGCTAT CTGACCTCAG AACAGCTCTT CAAACTGCTC
    GACTGTCTGC TGGAGTCTCA TCGATTTGCA AAGGCCTTCA ACTCCAATAA TGAGCAAAGA
    ACTGCTCTGT GGAAAGCCGG TTTCAAAGGG AAGTCGAAGC CCAATCTGCT GAAACAGGAG
    ACGAGCAGCC TGGCGTGTGG TCTGCGCATC CTCTTCCGCA TGTACACAGA TCCAAGCCGA
    CGGGATGCCT GGGAGGAGGT GCAGAGGAGA CTGCTCAACG TTTGCAGTGA CGCAGTGGCT
    TATTTTCTGA CCCTCACGTC TGAAAGTCAT CGCGAAGCCT GGACAAACCT TCTGCTGCTC
    TTCCTGACCA AAGTGCTGAA GATCAGTGAT GATAGATTCA AGGCTCATGC GTCCCGCTAC
    TACCCTCTGC TCTGTGAAAT CATGCAGTTC GACCTGATCC CGGAGCTGCG GGCCGTCCTG
    CGCAAGTTTT TCCTGCGCAT TGGACTGGTT TTCCAGATCA CCAACAGCCT GGAAACAAAT
    CAAACTGTCA GCAACGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49267
    Clone ID Related Accession (Same CDS sequence) XM_009297399.1
    Accession Version XM_009297399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG
    GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT
    CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC
    GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA
    CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC
    TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG
    CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC
    TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT
    AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC
    CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT
    TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA
    ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT
    TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA
    CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG
    AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA
    TACGTCAACC CCAATTCCCA GACAAGTCTT GGCCAAGAAA AGCCTTCAGA GCAGGAGAGC
    AATGAGTCGA AGCATCCAGA AACGATCAAC CGTTACGGCA GCATTAACTC TTTGGACTCC
    ACTGCATCAT CAGGCATTGG GAGCTACAGC ACACAGATGT CCGGAACAGA CAACCCGGAG
    CAGTTTGAGG TCCTCAAACA ACAGAAGGAG ATCATAGAGC AAGGCATTGA CCTGTTCAAC
    AAAAAACCAA AGAGGGGCAT TCAGTACCTG CAGGAGCAAG GCATGCTGGG AACAACGCCT
    GAAGATATTG CCCAGTTTTT ACATCAAGAG GAGAGACTTG ACTCTATCCA GGTAGGAGAG
    TTTCTAGGAG ACAACGACCG CATCAATAAA GAGGTCATGT ATGCCTACGT CGACCAAATG
    GACTTCCAGG GCAAAGACTT CGTGCCAGCG CTGCGCATGT TCCTGGAGGG TTTCCGTCTG
    CCCGGCGAAG CCCAGAAAAT TGATCGACTG ATGGAGAAAT TTGCTGCTAG ATATTTAGAA
    TGCAACCAAG GGCAAACACT GTTCGCCAGC GCTGACACCG CATACGTTCT TGCGTATTCA
    ATCATCATGT TGACTACCGA TCTGCACAGT CCACAGGTGA AGAATAAAAT GACGAAAGAG
    CAATACATCA AGATGAATCG AGGTATCAAT GACAGTAAAG ATTTACCGGA GGAGTATCTC
    TCAGCCATTT ATGATGAAAT CGCAGGGAAG AAAATCTCCA TGAAGGAGAC AAAGGAGTTA
    ACTCTCAAAT CAAATAAGCA AAGTGTGGCG AGTGAGAAGC AGAGGAGGCT GCTGTATAAT
    GTTGAGATGG AGCAGATGGC AAAAACAGCA AAGGCTCTGA TGGAGGCCGT GAGCCACGTT
    CAAGCCCCCT TCACCAGCGC CACACATCTT GAGCATGTCA GACCAATGTT TAAGCTGGCC
    TGGACGCCCT TTCTGGCTGC TTTCAGTGTG GGTCTGCAGG ACTGTGATGA CACTGAAGTG
    GCCTCTCTGT GTCTGGAGGG AATCCGCTGT GCCATCAGGA TAGCATGCAT CTTCAGCATT
    CAGTTGGAGC GAGACGCATA TGTACAGGCT CTGGCCCGTT TCACACTCTT GACGGCCAGT
    TCTGGCATCA CTGAAATGAA GCAGAAGAAC ATCGACACCA TCAAGACCCT CATCACAGTG
    GCTCACACTG ATGGAAATTA TCTAGGAAAC TCCTGGCATG AGATCATGAA GTGCATTAGT
    CAGCTGGAGT TGGCCCAGCT CATCGGGACA GGAGTGAAGG CTCGGTACAT ATCAGGAACT
    GTTCGGGGAA AGGAAGGCTT TATCACCAGC ACCAAGGAAC AAACGTCTGA TGAATACCTG
    GGATTGGGAG GGAATGTTGA TCGCAAACAG ATTGCAAGCA TTCAGGAGTC TATCGGGGAG
    ACCAGCTCGC AAAGTGTAGT TGTTGCTGTA GACCGGATAT TCACGGGCTC TACCAGACTT
    GATGGAAATG CCATTGTGGA CTTTGTGCGA TGGCTGTGCG CTGTCTCGAT GGATGAACTG
    GCCTCCCCAA CACACCCTCG AATGTTCAGT CTGCAGAAGA TCGTGGAGAT TTCATACTAC
    AATATGGGTC GTATCAGGTT GCAGTGGTCC AGGATTTGGG AGGTTATAGG GGATCATTTC
    AATAAGGTTG GCTGTAATCC CAACGAAGAT GTTGCTATTT TTGCTGTGGA TTCTCTGAGG
    CAGTTGTCCA TGAAGTTTTT GGAAAAGGGA GAGTTGGCCA ATTTCAGATT CCAGAAGGAT
    TTCCTCAGGC CGTTCGAGCA CATAATGAAA AAGAACAGAT CTCCGACCAT TAGAGACATG
    GTGGTTCGCT GTATTGCCCA GATGGTCAAC TCTCAGGCTG GCAATATTAG ATCAGGGTGG
    AAGAACATTT TCTCTGTGTT TCATCTGGCT GCTTCTGATC AGGATGAGAG TATAGTTGAG
    CTGGCCTTCC AGACAACTGG ACACATCGTC ACGAATGTAT TTGAGAAGCA TTTCCCAGCT
    ACCATTGACT CCTTCCAGGA CGCTGTCAAG TGTTTGTCTG AGTTCGCCTG CAACGCTTCT
    TTTCCAGACA CCAGCATGGA GGCTATTCGT CTGATCCGCC ACTGCGCCAA ATATGTGTCA
    GACCGACCCC AGGCGTTTAA AGACTATACC AGTGATGATA TGAATGTTGC CCCGGAGGAC
    CGTGTTTGGG TCAGAGGCTG GTTCCCCATC CTTTTTGAGC TGTCATGCAT CATCAACAGA
    TGCAAACTGG ACGTCCGGAC CAGGGGTCTG ACAGTGATGT TTGAGGTGAT GAAGACGTAC
    GGTCATACCT ATGAGAAACA CTGGTGGCAG GATCTGTTCC GCATCGTCTT CAGGATCTTC
    GACAACATGA AGCTTCCTGA GCAACAGACC GAGAAAGCGG AGTGGATGAC CACCACCTGC
    AATCATGCAC TTTATGCCAT CTGTGACGTT TTTACACAGT ACTTTGAGTC TCTCAGTGAT
    GTTCTTTTAG ATGATATCTT ATCTCAGCTC TACTGGTGTG TCCAGCAAGA TAATGAGCAA
    TTAGCCAGAT CAGGCACTAA CTGCCTTGAA AACGTGGTCA TCCTTAATGG TGAGAAGTTC
    AATCCTGAGA CTTGGGACAA GACATGCAAC TGCATGCTGG ACATCTTCAA AACCACCATC
    CCACACATGC TGCTGACTTG GAGACCGGCT GGTGCTGAAG GAGATCACAT GACTCAGCTG
    GAGTCTGATA AACAGCTGGA CTCCATTTCC CAGAAGTCAG TGGATATTCA GACCCGCTCA
    GATGACCAGC AGTCTGTTAA CAGTATGGAG AAGGCTTTGG CTGACAACCG CAGATACAGT
    CAGTTCAGTG TGGCTTCAGA AGCTCAGGAA CAGAGGCTGT TTGCTGCGCT ACTCATTAAG
    TGTGTGGTTC AGCTGGAGCT CATTCAGACC ATTGACAACA TCGTCTTCTT TCCTGCTACC
    AGTAAGAAAG AAGATGCGGA AAACTTTGCG GCTGCACAGA GAGATGCTTT AGATGCTGAT
    ATACATGTGG ACACACAGGA CCAGGGAATG TATCGCTATC TGACCTCAGA ACAGCTCTTC
    AAACTGCTCG ACTGTCTGCT GGAGTCTCAT CGATTTGCAA AGGCCTTCAA CTCCAATAAT
    GAGCAAAGAA CTGCTCTGTG GAAAGCCGGT TTCAAAGGGA AGTCGAAGCC CAATCTGCTG
    AAACAGGAGA CGAGCAGCCT GGCGTGTGGT CTGCGCATCC TCTTCCGCAT GTACACAGAT
    CCAAGCCGAC GGGATGCCTG GGAGGAGGTG CAGAGGAGAC TGCTCAACGT TTGCAGTGAC
    GCAGTGGCTT ATTTTCTGAC CCTCACGTCT GAAAGTCATC GCGAAGCCTG GACAAACCTT
    CTGCTGCTCT TCCTGACCAA AGTGCTGAAG ATCAGTGATG ATAGATTCAA GGCTCATGCG
    TCCCGCTACT ACCCTCTGCT CTGTGAAATC ATGCAGTTCG ACCTGATCCC GGAGCTGCGG
    GCCGTCCTGC GCAAGTTTTT CCTGCGCATT GGACTGGTTT TCCAGATCAC CAACAGCCTG
    GAAACAAATC AAACTGTCAG CAACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295674.1
    CDS206..5755
    Translation

    Target ORF information:

    RefSeq Version XM_009297399.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
    GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA
    TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG
    AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC
    TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA
    GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC
    ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC
    CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA
    TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC
    ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG
    ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG
    GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA
    CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA
    CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT
    GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA
    GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT
    GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT
    ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG
    GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT
    CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC
    GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA
    CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC
    TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG
    CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC
    TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT
    AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC
    CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT
    TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA
    ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT
    TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA
    CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG
    AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA
    TACGTCAACC CCAATTCCCA GACAAGTCTT GGCCAAGAAA AGCCTTCAGA GCAGGAGAGC
    AATGAGTCGA AGCATCCAGA AACGATCAAC CGTTACGGCA GCATTAACTC TTTGGACTCC
    ACTGCATCAT CAGGCATTGG GAGCTACAGC ACACAGATGT CCGGAACAGA CAACCCGGAG
    CAGTTTGAGG TCCTCAAACA ACAGAAGGAG ATCATAGAGC AAGGCATTGA CCTGTTCAAC
    AAAAAACCAA AGAGGGGCAT TCAGTACCTG CAGGAGCAAG GCATGCTGGG AACAACGCCT
    GAAGATATTG CCCAGTTTTT ACATCAAGAG GAGAGACTTG ACTCTATCCA GGTAGGAGAG
    TTTCTAGGAG ACAACGACCG CATCAATAAA GAGGTCATGT ATGCCTACGT CGACCAAATG
    GACTTCCAGG GCAAAGACTT CGTGCCAGCG CTGCGCATGT TCCTGGAGGG TTTCCGTCTG
    CCCGGCGAAG CCCAGAAAAT TGATCGACTG ATGGAGAAAT TTGCTGCTAG ATATTTAGAA
    TGCAACCAAG GGCAAACACT GTTCGCCAGC GCTGACACCG CATACGTTCT TGCGTATTCA
    ATCATCATGT TGACTACCGA TCTGCACAGT CCACAGGTGA AGAATAAAAT GACGAAAGAG
    CAATACATCA AGATGAATCG AGGTATCAAT GACAGTAAAG ATTTACCGGA GGAGTATCTC
    TCAGCCATTT ATGATGAAAT CGCAGGGAAG AAAATCTCCA TGAAGGAGAC AAAGGAGTTA
    ACTCTCAAAT CAAATAAGCA AAGTGTGGCG AGTGAGAAGC AGAGGAGGCT GCTGTATAAT
    GTTGAGATGG AGCAGATGGC AAAAACAGCA AAGGCTCTGA TGGAGGCCGT GAGCCACGTT
    CAAGCCCCCT TCACCAGCGC CACACATCTT GAGCATGTCA GACCAATGTT TAAGCTGGCC
    TGGACGCCCT TTCTGGCTGC TTTCAGTGTG GGTCTGCAGG ACTGTGATGA CACTGAAGTG
    GCCTCTCTGT GTCTGGAGGG AATCCGCTGT GCCATCAGGA TAGCATGCAT CTTCAGCATT
    CAGTTGGAGC GAGACGCATA TGTACAGGCT CTGGCCCGTT TCACACTCTT GACGGCCAGT
    TCTGGCATCA CTGAAATGAA GCAGAAGAAC ATCGACACCA TCAAGACCCT CATCACAGTG
    GCTCACACTG ATGGAAATTA TCTAGGAAAC TCCTGGCATG AGATCATGAA GTGCATTAGT
    CAGCTGGAGT TGGCCCAGCT CATCGGGACA GGAGTGAAGG CTCGGTACAT ATCAGGAACT
    GTTCGGGGAA AGGAAGGCTT TATCACCAGC ACCAAGGAAC AAACGTCTGA TGAATACCTG
    GGATTGGGAG GGAATGTTGA TCGCAAACAG ATTGCAAGCA TTCAGGAGTC TATCGGGGAG
    ACCAGCTCGC AAAGTGTAGT TGTTGCTGTA GACCGGATAT TCACGGGCTC TACCAGACTT
    GATGGAAATG CCATTGTGGA CTTTGTGCGA TGGCTGTGCG CTGTCTCGAT GGATGAACTG
    GCCTCCCCAA CACACCCTCG AATGTTCAGT CTGCAGAAGA TCGTGGAGAT TTCATACTAC
    AATATGGGTC GTATCAGGTT GCAGTGGTCC AGGATTTGGG AGGTTATAGG GGATCATTTC
    AATAAGGTTG GCTGTAATCC CAACGAAGAT GTTGCTATTT TTGCTGTGGA TTCTCTGAGG
    CAGTTGTCCA TGAAGTTTTT GGAAAAGGGA GAGTTGGCCA ATTTCAGATT CCAGAAGGAT
    TTCCTCAGGC CGTTCGAGCA CATAATGAAA AAGAACAGAT CTCCGACCAT TAGAGACATG
    GTGGTTCGCT GTATTGCCCA GATGGTCAAC TCTCAGGCTG GCAATATTAG ATCAGGGTGG
    AAGAACATTT TCTCTGTGTT TCATCTGGCT GCTTCTGATC AGGATGAGAG TATAGTTGAG
    CTGGCCTTCC AGACAACTGG ACACATCGTC ACGAATGTAT TTGAGAAGCA TTTCCCAGCT
    ACCATTGACT CCTTCCAGGA CGCTGTCAAG TGTTTGTCTG AGTTCGCCTG CAACGCTTCT
    TTTCCAGACA CCAGCATGGA GGCTATTCGT CTGATCCGCC ACTGCGCCAA ATATGTGTCA
    GACCGACCCC AGGCGTTTAA AGACTATACC AGTGATGATA TGAATGTTGC CCCGGAGGAC
    CGTGTTTGGG TCAGAGGCTG GTTCCCCATC CTTTTTGAGC TGTCATGCAT CATCAACAGA
    TGCAAACTGG ACGTCCGGAC CAGGGGTCTG ACAGTGATGT TTGAGGTGAT GAAGACGTAC
    GGTCATACCT ATGAGAAACA CTGGTGGCAG GATCTGTTCC GCATCGTCTT CAGGATCTTC
    GACAACATGA AGCTTCCTGA GCAACAGACC GAGAAAGCGG AGTGGATGAC CACCACCTGC
    AATCATGCAC TTTATGCCAT CTGTGACGTT TTTACACAGT ACTTTGAGTC TCTCAGTGAT
    GTTCTTTTAG ATGATATCTT ATCTCAGCTC TACTGGTGTG TCCAGCAAGA TAATGAGCAA
    TTAGCCAGAT CAGGCACTAA CTGCCTTGAA AACGTGGTCA TCCTTAATGG TGAGAAGTTC
    AATCCTGAGA CTTGGGACAA GACATGCAAC TGCATGCTGG ACATCTTCAA AACCACCATC
    CCACACATGC TGCTGACTTG GAGACCGGCT GGTGCTGAAG GAGATCACAT GACTCAGCTG
    GAGTCTGATA AACAGCTGGA CTCCATTTCC CAGAAGTCAG TGGATATTCA GACCCGCTCA
    GATGACCAGC AGTCTGTTAA CAGTATGGAG AAGGCTTTGG CTGACAACCG CAGATACAGT
    CAGTTCAGTG TGGCTTCAGA AGCTCAGGAA CAGAGGCTGT TTGCTGCGCT ACTCATTAAG
    TGTGTGGTTC AGCTGGAGCT CATTCAGACC ATTGACAACA TCGTCTTCTT TCCTGCTACC
    AGTAAGAAAG AAGATGCGGA AAACTTTGCG GCTGCACAGA GAGATGCTTT AGATGCTGAT
    ATACATGTGG ACACACAGGA CCAGGGAATG TATCGCTATC TGACCTCAGA ACAGCTCTTC
    AAACTGCTCG ACTGTCTGCT GGAGTCTCAT CGATTTGCAA AGGCCTTCAA CTCCAATAAT
    GAGCAAAGAA CTGCTCTGTG GAAAGCCGGT TTCAAAGGGA AGTCGAAGCC CAATCTGCTG
    AAACAGGAGA CGAGCAGCCT GGCGTGTGGT CTGCGCATCC TCTTCCGCAT GTACACAGAT
    CCAAGCCGAC GGGATGCCTG GGAGGAGGTG CAGAGGAGAC TGCTCAACGT TTGCAGTGAC
    GCAGTGGCTT ATTTTCTGAC CCTCACGTCT GAAAGTCATC GCGAAGCCTG GACAAACCTT
    CTGCTGCTCT TCCTGACCAA AGTGCTGAAG ATCAGTGATG ATAGATTCAA GGCTCATGCG
    TCCCGCTACT ACCCTCTGCT CTGTGAAATC ATGCAGTTCG ACCTGATCCC GGAGCTGCGG
    GCCGTCCTGC GCAAGTTTTT CCTGCGCATT GGACTGGTTT TCCAGATCAC CAACAGCCTG
    GAAACAAATC AAACTGTCAG CAACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.