NCU07658 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07658
Entrez Gene ID	3878933
Full Name	protein transporter sec72
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_957692.3	XP_962785.3	protein transporter sec72

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07658 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07658 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12249	XM_957692.3 Latest version!	Neurospora crassa OR74A protein transporter sec72 (NCU07658), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07658 ( XM_957692.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_962785.3 Neurospora crassa OR74A protein transporter sec72 (NCU07658), mRNA.

Price & Availability↑

CloneID	ONf12249
Clone ID Related Accession (Same CDS sequence)	XM_957692.3
Accession Version	XM_957692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	protein transporter sec72
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957692.2.

ATGAGTTCCC TCAAGTTCGT GACGTCCTCT CTAGACGTCA TTGCCGCCAA TGCCGGCAAG 
AATAAGGAGC TGGCCGAATT GGCGCAAAAG GCCCAGGAAG CCATCAAGAC AAACGACCAG 
CAGCTCCCCG ACCCCGAGGT CATCTTCGCC CCCCTCCGAC TAGCCACCAA GTCCGGCACC 
ATCCCCTTGA CTACAACCGC CCTCGACTGC ATCGGCAAGC TCATCTCCTC CACCTACTTC 
TCCGTCCCTT CCGGCCGGTC CGCGGCGGCG AGCGAGGATG GCCAACCACA ACAGCAGCAA 
TACCTGCCCC TAATCGAACG CGCCATCGAC ACCATCTGCG ACTGCTTCCA GGGCGAGGCC 
ACCCTGGTCG AGATCCAGCT ACAGATTGTC AAGTCCCTCT TGGCCGCCGT CCTCAATGAC 
AAGATTGTCG TCCACGGCGC CGGCCTGCTC AAGGCTGTGC GCCAGGTTTA CAACATCTTC 
CTCCTTTCCA GGAGTACGGC CAACCAGCAG GTCGCCCAGG GGACCCTCAC ACAAATGGTC 
GGAACCGTGT TCGAGAGAGT CAGCGCCAGG CTGCACATGA GGGAGGCCCG CGCCAATCTG 
AGCAAGCTGA AGCGGAGCCA GAGCAGTTTC AACGTCAACG GCTCATCCGA CGGTCAAAAT 
TCCACACAAG AGGAGGCCAA CGGAGACGAT GACGAGAACG TCCCCGAAGA TGCGTCCGAA 
TCGCAATTAC AAGATGGGGA TGGCAACGGC GACGGCCCCA AGCTCACCCT CAAGGACCTC 
GAGCACCGCA AGAGCTTCGA CGACTCCAAT CTCGGAGATG GCCCTACCAT GGTCACCCAG 
CTAAAACCCA ACAGGAAGCC TGCGAGGTCC GTGTCGGAAC AGAGCGTGCC CGAGAGCGCC 
CAGGAGGACA GCCCCGAGTC GTTGGACGCC GAGGACGAAG TGTACATCCG CGACGCCTAC 
CTCGTCTTCC GCTCCTTCTG CAACCTCTCG ACCAAGATCC TGCCGCCCGA CCAGCTCTAC 
GACCTCCGCG GCCAGCCCAT GCGGTCCAAG CTCATCTCCC TCCACCTCAT CCACACCCTT 
CTCAACAACA ACATTTCTGT TTTCACTTCG CCCTACTGTA CCATCACCAA TACCAAGAAC 
AACGAGCCCA CCAGCTTTTT GCAGGCCACA AAGTACTATC TGTGTTTGAG TATCACACGG 
AACGGGGCTA GCTCGGTCGA TAGAGTATTT AATATTTGCT GTGAGATCTT TTGGCTAATG 
CTCAAGTACA TGAGGGCTCC ATTCAAGAAA GAAATCGAGG TCTTCTTGAA CGAAATCTAC 
CTAGCCCTTT TGGCGCGGAG GACCGCCCCC TTGTCCCAGA AGCTCGCCTT TGTCAGTATC 
CTCAAGAGGC TATGTGAGGA TCCCCGGGCA TTGGTCGAGT TCTACCTTAA TTACGATTGC 
GACCGAAATG TCGACAATAT TTTCCAGCGC ATCGTCGAGG ACTTGTCCAA GTTTGCGACG 
TCGTCTACGA CCGTCACGGC ACTACAAGAA CAGCAGTACG AGGAAAACCA TGCCAAGTAT 
GGGTCAGCCA GCGAGTGGCA GATGCGCAAC GTTTTGCCAC CACCACTTAC CGTTGCCCTG 
ATAGCCCAAA ATACCGACAC CGATGGCGAT ATCCCCAAGG ACTATGTTCT AAAGAGGCAA 
GCTCTGGATT CCCTTGTTGA GTCGCTTAGG TCGCTTCTCA ACTGGTCCCA TCCAGGGCGC 
CCAGAGGTCA TCACCACGGG AACGGGCATT TCCGAAAGAC GGCCGTCAAG CGATGAGATC 
CGGGAATCCA TGGACCCTTC GATCATGGGC GAGTCCCTAT CCCGTTTAGA CACCCCGACC 
ATGCCATCGA CACCGCTGCT TGACGATGAC CCCGACCAGC TGGAAAAGGA AAAGCAGAGG 
AAGACGGCTC TCGGAAACGC TATCCGCGTG TTCAACTACA AGCCCAAGAA CGGCATCAAA 
TTGTTGTTGA AGGAAGGCTT TATCCCCGAA GACACGCCCG ACGCCATTGC CAAGTTCTTG 
ATCAGCGAGG AGAGGCTGGA CAAGGCCCAG ATCGGCGAAT ACCTCGGTGA GGGTGATCAG 
AAGAACATTG ACATCATGCA CGCTTTCGTC GACACCATGG ACTTCACCAA GAAGCGCTTC 
GTGGATGCCT TGCGCCAGTT TCTTCAGGCT TTCCGCTTGC CCGGTGAGGC CCAAAAGATT 
GATCGTTTCA TGCTCAAGTT CGCCAACCGA TACATGCTGG GAAACCCCAA GGCCTTTGCC 
AATGCTGATA CCCCTTATGT GCTGGCGTAC TCCGTCATCA TGCTGAACAC GGATCTCCAC 
AGCAGCAAGA TTGTCAGGCG CATGACCAAG GAGGACTTTA TCAAGAACAA CCGCGGTATC 
AACGATAACG CCAACCTTCC GGACGAGTAC CTCATCAGCA TCTACGACGA TATTGCCAAC 
AACGAGATTG TCCTCAAGAG TGAGCGGGAA GCGGCGGCTG CGGCGGGAAC GCTTCCAGCA 
CAGTCAACCG GTCTGGCTGC TGGACTTGGC CAGGCCTTTT CCAACGTCGG TCGTGACCTG 
CAGAGGGAGG CATATGCTCA ACAGTCTGAA GAAATCGCCA TTCGGTCGGA GCAGCTCTTC 
AAGGACTTGT ACCGTAGTCA ACGGAAGAAT GCGCAAAAGA TGGGCGGCAT CAAGTTCATC 
CCCGCTACCT CGTTCAAGCA TGTATCGCCC ATGTTTGATG TGACGTGGAT GTCCTTCTTC 
TCGGCCCTGT CCAGCCAGAT GCAAAAGACA CATAACCTGG ATGTGAACAA GCTGTGTCTG 
GAGGGTATGA AGCTTGCGAC CAAGATTGCT TGCTTCTTTG ATCTCAGCAC TCCTAGAGAA 
GCCTTTATCT CGGCGCTCAA GAACACGGCC AATCTCAACA ACCCCCAGGA GATTCAAGCT 
AAGAACGTCG AGGCCCTCAA GGTGATTCTT GAGCTTGGAC AGACAGAAGG CAACCTCCTC 
AAGGAGTCGT GGAAGGATGT TCTCCTTTGT ATCAGCCAGC TTGATCGTCT CCAGCTCATC 
TCGGGTGGTG TCGACGAGAG TGCCGTCCCC GACGTCTCCA AGGCCCGTTT CGTTCCTCCA 
CCAAGAAGCG ACTCGACGGA CTCCCGCAAG TCTATGGCTG CCAAGCGACA TCGCCCACGA 
TCAAACACGG GCCCTCAGGG CGTCTCAATG GAAATTGCCC TCGAAAGCCG TTCGGACGAA 
GTCATTAAGA GCGTCGACCG CATCTTCACC AACACCGCCC ACCTCAGCGG CGAGGCCATT 
GTTCACTTTG CCCGTGCCTT GACCGAAGTC AGCTGGGATG AGATCAAGGT GTCCGGGTCG 
AACGACTCGC CGCGCACTTA CAGTCTGCAG AAGATTGTCG AGATTTCGTA CTACAACATG 
ACGCGTGTGA GGTTCGAGTG GACCAACATC TGGGATGTGT TGGGTGAGCA CTTCAACCGG 
GTCGGGTGCC ACGTCAATGC CGCTATCGTG TTCTTTGCTC TGGACTCGCT GCGTCAGCTG 
TCTACGCGCT TTATGGAGAT TGAGGAGTTG GCGGGTTTCA AGTTCCAGAA GGACTTTCTC 
AAGCCGTTTG AGCATGTCAT GTCCAACTCG AACAACATTA CTGTCAAGGA TATGATTTTG 
AGATGTCTGA TTCAGATGAT CCAGGCCAAG GGAGACAAGA TCCGTTCCGG CTGGAGGACC 
ATGTTTGGCG TCTTTACCGT GGCCGCCAAG GACCAGTACG AGAGCATCGT CAACCTGGCT 
TATGAGCACG TTCTGCATGT GTACAAGACG CGCTTCGGCG TGGTCATCAC CCAGGGCGCG 
TTTACCGATT TGATCGTCTG CTTGACCGAG TTTTCCAAGA ACATGAAGTT CCAGAAGAAG 
AGTCTGCAGG CGATGGAGAC GCTCAAGAGC ATCATTCCCA AGATGCTCAA GACACCCGAG 
TGTCCGCTGT CGCATAAGCA GAACTCGGAA GTAAACTCTG CCGAGATGGC TCTCAACGCT 
GCCACCAAGC CGACTGGTCA AGAAACGGGC ACAACGGTGG AGGAGGGTTT CTGGTTCCCT 
GTTCTGTTCG CCTTCCATGA CGTTCTCATG ACCGGCGAGG ATTTGGAAGT ACGGTCGAAC 
GCCCTCAACT ACTTCTTTGA TGCCCTGCTC AAGTATGGAG CCACATTCCC GCTTGGGTTC 
TGGGATATCT TGTGGAGGCA GCAGTTGTAT CCCATCTTCA TGGTGCTACG GTCGAAGCCC 
GAAATGTCCA ATGCTCTTAG CCACGAGGAG TTGTCGGTTT GGTTGTCCAC TACCATGATC 
CAAGCCCTGC GCAACATGAT CACTCTGTTC ACGCACTACT TCAACCCATT GGAGTACATG 
CTCGACCGGT TCCTCGAACT GCTGGCGCTC TGCATCCTTC AAGAAAACGA CACCATTGCT 
CGCATCGGCA GCAACTGCCT GCAGACCTTG ATCCTGCAAA ATGTCGAAAA GTTCACCCAC 
GAACACTGGC AAAAGATCGT CGACACCTTC TGCGAGTTAT TCGACAAGAC CACCGCGCAC 
CAGCTCTTCA AAGCGGCCAC CATTTCCACC CCCTTGTCAG CGTCCAACGG CCTCGACTAC 
GCCAGCCAAC TCAGTCCCTC CGCCGACGGT GCCGACGCCG ACACCCGCTC CCTCAAGATC 
AACGGCGACA ACGACGCCGG CTCCGAAACT TCCTCCATCC ACCAAACGCC CCACACCAAC 
GGCGGTATCA ACCCCGAAGA GGACTCCCAT TCCTCAGTTC ACAACCGTCT CACGGCTTCC 
ACCGCGCTTT CCTCGCACCC ACCCAACACC CCCGGCGGCC AGGGTCTCGA GGAATTCAAG 
CCATCCACCA CCCTTCAACA ACAACCGCCC GTCGTGACCG CCGCCCGCCG CCGCTTCTTC 
AACCGCATCA TCAGCCGCTG CGTCCTGCAA CTGCTCATGA TCGAGACCGT CCAAGAGCTC 
TTCTCCAACG ACTCGGTCTA CAGCCACATC CCCTCCCCGC TGCTCTTGAA ACTCATGTCC 
CTTTTGAAGC GCTCCTACCT GTTCGCCAAG CGCTTCAACG CCGACAAAGA CTTGCGCATG 
CGTCTCTGGC GCGAGGGATT CATGAAACAG CCGCCGAACC TGCTCAAACA GGAATCAGGC 
AGCGCCGCTG TCTATGTTTC GATCCTATTC AGGATGTTCG CCGACACCTC TCCCGAGCGT 
CTCGCGTCCA AGGCCGATGT CGAACGGGCT TTGGTCCCTT TATGTCTGGA CATTGTCCGC 
GTGTATGTCT CGCTGGAAGA AGAAAGTCAG AATAGGAATA TCCAGGCGTG GAGGCCCGTG 
GTGGTGGAGG TGTTGAATGG GTTTGCCGGG GGCGTTAGTA ACCCCGAAAA GGGCGAGATG 
AAGGAAGGCA GAGGGTTCGG AGAAGAAGAC TTCAAGAAAC ATTTGCATGA GGTGTACCCT 
GTGGTGATTG AGTTGCTGGG GAAGGAGTTG GGGCAAGGCG AGAGGGAACT GAGGCAGGCG 
TTGCTGGGGG TGTTGAGACG GGTGGGAGAG GTGGGGTTGG GGATTGAGGG GTTGGTTGGT 
AGCTCCAACT CTAACTCTAA CTCTACGGGG AGGTTGTCGT CGAATCACAG GCGACAGGAT 
ACGTTGGTGA TGAGTGGTAA TGGGAGGATG GCCTATGGGC ATGATAGGAC GGAGAGTGTG 
GTTAGTACTA TGACTGTGGG GACGATTGGT GGTGGTGGTG GTGGTGGTGG TTATCGGGAG 
AGGGAAAGGG AGAGGGAGGG AGAAAGAGAA GGGGAGGGAG AAGGGGAGGA TATGGCGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_962785.3
CDS	764..6586
Translation	MSSLKFVTSSLDVIAANAGKNKELAELAQKAQEAIKTNDQQLPDPEVIFAPLRLATKSGTIPLTTTALDCIGKLISSTYFSVPSGRSAAASEDGQPQQQQYLPLIERAIDTICDCFQGEATLVEIQLQIVKSLLAAVLNDKIVVHGAGLLKAVRQVYNIFLLSRSTANQQVAQGTLTQMVGTVFERVSARLHMREARANLSKLKRSQSSFNVNGSSDGQNSTQEEANGDDDENVPEDASESQLQDGDGNGDGPKLTLKDLEHRKSFDDSNLGDGPTMVTQLKPNRKPARSVSEQSVPESAQEDSPESLDAEDEVYIRDAYLVFRSFCNLSTKILPPDQLYDLRGQPMRSKLISLHLIHTLLNNNISVFTSPYCTITNTKNNEPTSFLQATKYYLCLSITRNGASSVDRVFNICCEIFWLMLKYMRAPFKKEIEVFLNEIYLALLARRTAPLSQKLAFVSILKRLCEDPRALVEFYLNYDCDRNVDNIFQRIVEDLSKFATSSTTVTALQEQQYEENHAKYGSASEWQMRNVLPPPLTVALIAQNTDTDGDIPKDYVLKRQALDSLVESLRSLLNWSHPGRPEVITTGTGISERRPSSDEIRESMDPSIMGESLSRLDTPTMPSTPLLDDDPDQLEKEKQRKTALGNAIRVFNYKPKNGIKLLLKEGFIPEDTPDAIAKFLISEERLDKAQIGEYLGEGDQKNIDIMHAFVDTMDFTKKRFVDALRQFLQAFRLPGEAQKIDRFMLKFANRYMLGNPKAFANADTPYVLAYSVIMLNTDLHSSKIVRRMTKEDFIKNNRGINDNANLPDEYLISIYDDIANNEIVLKSEREAAAAAGTLPAQSTGLAAGLGQAFSNVGRDLQREAYAQQSEEIAIRSEQLFKDLYRSQRKNAQKMGGIKFIPATSFKHVSPMFDVTWMSFFSALSSQMQKTHNLDVNKLCLEGMKLATKIACFFDLSTPREAFISALKNTANLNNPQEIQAKNVEALKVILELGQTEGNLLKESWKDVLLCISQLDRLQLISGGVDESAVPDVSKARFVPPPRSDSTDSRKSMAAKRHRPRSNTGPQGVSMEIALESRSDEVIKSVDRIFTNTAHLSGEAIVHFARALTEVSWDEIKVSGSNDSPRTYSLQKIVEISYYNMTRVRFEWTNIWDVLGEHFNRVGCHVNAAIVFFALDSLRQLSTRFMEIEELAGFKFQKDFLKPFEHVMSNSNNITVKDMILRCLIQMIQAKGDKIRSGWRTMFGVFTVAAKDQYESIVNLAYEHVLHVYKTRFGVVITQGAFTDLIVCLTEFSKNMKFQKKSLQAMETLKSIIPKMLKTPECPLSHKQNSEVNSAEMALNAATKPTGQETGTTVEEGFWFPVLFAFHDVLMTGEDLEVRSNALNYFFDALLKYGATFPLGFWDILWRQQLYPIFMVLRSKPEMSNALSHEELSVWLSTTMIQALRNMITLFTHYFNPLEYMLDRFLELLALCILQENDTIARIGSNCLQTLILQNVEKFTHEHWQKIVDTFCELFDKTTAHQLFKAATISTPLSASNGLDYASQLSPSADGADADTRSLKINGDNDAGSETSSIHQTPHTNGGINPEEDSHSSVHNRLTASTALSSHPPNTPGGQGLEEFKPSTTLQQQPPVVTAARRRFFNRIISRCVLQLLMIETVQELFSNDSVYSHIPSPLLLKLMSLLKRSYLFAKRFNADKDLRMRLWREGFMKQPPNLLKQESGSAAVYVSILFRMFADTSPERLASKADVERALVPLCLDIVRVYVSLEEESQNRNIQAWRPVVVEVLNGFAGGVSNPEKGEMKEGRGFGEEDFKKHLHEVYPVVIELLGKELGQGERELRQALLGVLRRVGEVGLGIEGLVGSSNSNSNSTGRLSSNHRRQDTLVMSGNGRMAYGHDRTESVVSTMTVGTIGGGGGGGGYREREREREGEREGEGEGEDMAR

Target ORF information:

RefSeq Version	XM_957692.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A protein transporter sec72 (NCU07658), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_957692.3

ORF Insert Sequence:

ATGAGTTCCC TCAAGTTCGT GACGTCCTCT CTAGACGTCA TTGCCGCCAA TGCCGGCAAG 
AATAAGGAGC TGGCCGAATT GGCGCAAAAG GCCCAGGAAG CCATCAAGAC AAACGACCAG 
CAGCTCCCCG ACCCCGAGGT CATCTTCGCC CCCCTCCGAC TAGCCACCAA GTCCGGCACC 
ATCCCCTTGA CTACAACCGC CCTCGACTGC ATCGGCAAGC TCATCTCCTC CACCTACTTC 
TCCGTCCCTT CCGGCCGGTC CGCGGCGGCG AGCGAGGATG GCCAACCACA ACAGCAGCAA 
TACCTGCCCC TAATCGAACG CGCCATCGAC ACCATCTGCG ACTGCTTCCA GGGCGAGGCC 
ACCCTGGTCG AGATCCAGCT ACAGATTGTC AAGTCCCTCT TGGCCGCCGT CCTCAATGAC 
AAGATTGTCG TCCACGGCGC CGGCCTGCTC AAGGCTGTGC GCCAGGTTTA CAACATCTTC 
CTCCTTTCCA GGAGTACGGC CAACCAGCAG GTCGCCCAGG GGACCCTCAC ACAAATGGTC 
GGAACCGTGT TCGAGAGAGT CAGCGCCAGG CTGCACATGA GGGAGGCCCG CGCCAATCTG 
AGCAAGCTGA AGCGGAGCCA GAGCAGTTTC AACGTCAACG GCTCATCCGA CGGTCAAAAT 
TCCACACAAG AGGAGGCCAA CGGAGACGAT GACGAGAACG TCCCCGAAGA TGCGTCCGAA 
TCGCAATTAC AAGATGGGGA TGGCAACGGC GACGGCCCCA AGCTCACCCT CAAGGACCTC 
GAGCACCGCA AGAGCTTCGA CGACTCCAAT CTCGGAGATG GCCCTACCAT GGTCACCCAG 
CTAAAACCCA ACAGGAAGCC TGCGAGGTCC GTGTCGGAAC AGAGCGTGCC CGAGAGCGCC 
CAGGAGGACA GCCCCGAGTC GTTGGACGCC GAGGACGAAG TGTACATCCG CGACGCCTAC 
CTCGTCTTCC GCTCCTTCTG CAACCTCTCG ACCAAGATCC TGCCGCCCGA CCAGCTCTAC 
GACCTCCGCG GCCAGCCCAT GCGGTCCAAG CTCATCTCCC TCCACCTCAT CCACACCCTT 
CTCAACAACA ACATTTCTGT TTTCACTTCG CCCTACTGTA CCATCACCAA TACCAAGAAC 
AACGAGCCCA CCAGCTTTTT GCAGGCCACA AAGTACTATC TGTGTTTGAG TATCACACGG 
AACGGGGCTA GCTCGGTCGA TAGAGTATTT AATATTTGCT GTGAGATCTT TTGGCTAATG 
CTCAAGTACA TGAGGGCTCC ATTCAAGAAA GAAATCGAGG TCTTCTTGAA CGAAATCTAC 
CTAGCCCTTT TGGCGCGGAG GACCGCCCCC TTGTCCCAGA AGCTCGCCTT TGTCAGTATC 
CTCAAGAGGC TATGTGAGGA TCCCCGGGCA TTGGTCGAGT TCTACCTTAA TTACGATTGC 
GACCGAAATG TCGACAATAT TTTCCAGCGC ATCGTCGAGG ACTTGTCCAA GTTTGCGACG 
TCGTCTACGA CCGTCACGGC ACTACAAGAA CAGCAGTACG AGGAAAACCA TGCCAAGTAT 
GGGTCAGCCA GCGAGTGGCA GATGCGCAAC GTTTTGCCAC CACCACTTAC CGTTGCCCTG 
ATAGCCCAAA ATACCGACAC CGATGGCGAT ATCCCCAAGG ACTATGTTCT AAAGAGGCAA 
GCTCTGGATT CCCTTGTTGA GTCGCTTAGG TCGCTTCTCA ACTGGTCCCA TCCAGGGCGC 
CCAGAGGTCA TCACCACGGG AACGGGCATT TCCGAAAGAC GGCCGTCAAG CGATGAGATC 
CGGGAATCCA TGGACCCTTC GATCATGGGC GAGTCCCTAT CCCGTTTAGA CACCCCGACC 
ATGCCATCGA CACCGCTGCT TGACGATGAC CCCGACCAGC TGGAAAAGGA AAAGCAGAGG 
AAGACGGCTC TCGGAAACGC TATCCGCGTG TTCAACTACA AGCCCAAGAA CGGCATCAAA 
TTGTTGTTGA AGGAAGGCTT TATCCCCGAA GACACGCCCG ACGCCATTGC CAAGTTCTTG 
ATCAGCGAGG AGAGGCTGGA CAAGGCCCAG ATCGGCGAAT ACCTCGGTGA GGGTGATCAG 
AAGAACATTG ACATCATGCA CGCTTTCGTC GACACCATGG ACTTCACCAA GAAGCGCTTC 
GTGGATGCCT TGCGCCAGTT TCTTCAGGCT TTCCGCTTGC CCGGTGAGGC CCAAAAGATT 
GATCGTTTCA TGCTCAAGTT CGCCAACCGA TACATGCTGG GAAACCCCAA GGCCTTTGCC 
AATGCTGATA CCCCTTATGT GCTGGCGTAC TCCGTCATCA TGCTGAACAC GGATCTCCAC 
AGCAGCAAGA TTGTCAGGCG CATGACCAAG GAGGACTTTA TCAAGAACAA CCGCGGTATC 
AACGATAACG CCAACCTTCC GGACGAGTAC CTCATCAGCA TCTACGACGA TATTGCCAAC 
AACGAGATTG TCCTCAAGAG TGAGCGGGAA GCGGCGGCTG CGGCGGGAAC GCTTCCAGCA 
CAGTCAACCG GTCTGGCTGC TGGACTTGGC CAGGCCTTTT CCAACGTCGG TCGTGACCTG 
CAGAGGGAGG CATATGCTCA ACAGTCTGAA GAAATCGCCA TTCGGTCGGA GCAGCTCTTC 
AAGGACTTGT ACCGTAGTCA ACGGAAGAAT GCGCAAAAGA TGGGCGGCAT CAAGTTCATC 
CCCGCTACCT CGTTCAAGCA TGTATCGCCC ATGTTTGATG TGACGTGGAT GTCCTTCTTC 
TCGGCCCTGT CCAGCCAGAT GCAAAAGACA CATAACCTGG ATGTGAACAA GCTGTGTCTG 
GAGGGTATGA AGCTTGCGAC CAAGATTGCT TGCTTCTTTG ATCTCAGCAC TCCTAGAGAA 
GCCTTTATCT CGGCGCTCAA GAACACGGCC AATCTCAACA ACCCCCAGGA GATTCAAGCT 
AAGAACGTCG AGGCCCTCAA GGTGATTCTT GAGCTTGGAC AGACAGAAGG CAACCTCCTC 
AAGGAGTCGT GGAAGGATGT TCTCCTTTGT ATCAGCCAGC TTGATCGTCT CCAGCTCATC 
TCGGGTGGTG TCGACGAGAG TGCCGTCCCC GACGTCTCCA AGGCCCGTTT CGTTCCTCCA 
CCAAGAAGCG ACTCGACGGA CTCCCGCAAG TCTATGGCTG CCAAGCGACA TCGCCCACGA 
TCAAACACGG GCCCTCAGGG CGTCTCAATG GAAATTGCCC TCGAAAGCCG TTCGGACGAA 
GTCATTAAGA GCGTCGACCG CATCTTCACC AACACCGCCC ACCTCAGCGG CGAGGCCATT 
GTTCACTTTG CCCGTGCCTT GACCGAAGTC AGCTGGGATG AGATCAAGGT GTCCGGGTCG 
AACGACTCGC CGCGCACTTA CAGTCTGCAG AAGATTGTCG AGATTTCGTA CTACAACATG 
ACGCGTGTGA GGTTCGAGTG GACCAACATC TGGGATGTGT TGGGTGAGCA CTTCAACCGG 
GTCGGGTGCC ACGTCAATGC CGCTATCGTG TTCTTTGCTC TGGACTCGCT GCGTCAGCTG 
TCTACGCGCT TTATGGAGAT TGAGGAGTTG GCGGGTTTCA AGTTCCAGAA GGACTTTCTC 
AAGCCGTTTG AGCATGTCAT GTCCAACTCG AACAACATTA CTGTCAAGGA TATGATTTTG 
AGATGTCTGA TTCAGATGAT CCAGGCCAAG GGAGACAAGA TCCGTTCCGG CTGGAGGACC 
ATGTTTGGCG TCTTTACCGT GGCCGCCAAG GACCAGTACG AGAGCATCGT CAACCTGGCT 
TATGAGCACG TTCTGCATGT GTACAAGACG CGCTTCGGCG TGGTCATCAC CCAGGGCGCG 
TTTACCGATT TGATCGTCTG CTTGACCGAG TTTTCCAAGA ACATGAAGTT CCAGAAGAAG 
AGTCTGCAGG CGATGGAGAC GCTCAAGAGC ATCATTCCCA AGATGCTCAA GACACCCGAG 
TGTCCGCTGT CGCATAAGCA GAACTCGGAA GTAAACTCTG CCGAGATGGC TCTCAACGCT 
GCCACCAAGC CGACTGGTCA AGAAACGGGC ACAACGGTGG AGGAGGGTTT CTGGTTCCCT 
GTTCTGTTCG CCTTCCATGA CGTTCTCATG ACCGGCGAGG ATTTGGAAGT ACGGTCGAAC 
GCCCTCAACT ACTTCTTTGA TGCCCTGCTC AAGTATGGAG CCACATTCCC GCTTGGGTTC 
TGGGATATCT TGTGGAGGCA GCAGTTGTAT CCCATCTTCA TGGTGCTACG GTCGAAGCCC 
GAAATGTCCA ATGCTCTTAG CCACGAGGAG TTGTCGGTTT GGTTGTCCAC TACCATGATC 
CAAGCCCTGC GCAACATGAT CACTCTGTTC ACGCACTACT TCAACCCATT GGAGTACATG 
CTCGACCGGT TCCTCGAACT GCTGGCGCTC TGCATCCTTC AAGAAAACGA CACCATTGCT 
CGCATCGGCA GCAACTGCCT GCAGACCTTG ATCCTGCAAA ATGTCGAAAA GTTCACCCAC 
GAACACTGGC AAAAGATCGT CGACACCTTC TGCGAGTTAT TCGACAAGAC CACCGCGCAC 
CAGCTCTTCA AAGCGGCCAC CATTTCCACC CCCTTGTCAG CGTCCAACGG CCTCGACTAC 
GCCAGCCAAC TCAGTCCCTC CGCCGACGGT GCCGACGCCG ACACCCGCTC CCTCAAGATC 
AACGGCGACA ACGACGCCGG CTCCGAAACT TCCTCCATCC ACCAAACGCC CCACACCAAC 
GGCGGTATCA ACCCCGAAGA GGACTCCCAT TCCTCAGTTC ACAACCGTCT CACGGCTTCC 
ACCGCGCTTT CCTCGCACCC ACCCAACACC CCCGGCGGCC AGGGTCTCGA GGAATTCAAG 
CCATCCACCA CCCTTCAACA ACAACCGCCC GTCGTGACCG CCGCCCGCCG CCGCTTCTTC 
AACCGCATCA TCAGCCGCTG CGTCCTGCAA CTGCTCATGA TCGAGACCGT CCAAGAGCTC 
TTCTCCAACG ACTCGGTCTA CAGCCACATC CCCTCCCCGC TGCTCTTGAA ACTCATGTCC 
CTTTTGAAGC GCTCCTACCT GTTCGCCAAG CGCTTCAACG CCGACAAAGA CTTGCGCATG 
CGTCTCTGGC GCGAGGGATT CATGAAACAG CCGCCGAACC TGCTCAAACA GGAATCAGGC 
AGCGCCGCTG TCTATGTTTC GATCCTATTC AGGATGTTCG CCGACACCTC TCCCGAGCGT 
CTCGCGTCCA AGGCCGATGT CGAACGGGCT TTGGTCCCTT TATGTCTGGA CATTGTCCGC 
GTGTATGTCT CGCTGGAAGA AGAAAGTCAG AATAGGAATA TCCAGGCGTG GAGGCCCGTG 
GTGGTGGAGG TGTTGAATGG GTTTGCCGGG GGCGTTAGTA ACCCCGAAAA GGGCGAGATG 
AAGGAAGGCA GAGGGTTCGG AGAAGAAGAC TTCAAGAAAC ATTTGCATGA GGTGTACCCT 
GTGGTGATTG AGTTGCTGGG GAAGGAGTTG GGGCAAGGCG AGAGGGAACT GAGGCAGGCG 
TTGCTGGGGG TGTTGAGACG GGTGGGAGAG GTGGGGTTGG GGATTGAGGG GTTGGTTGGT 
AGCTCCAACT CTAACTCTAA CTCTACGGGG AGGTTGTCGT CGAATCACAG GCGACAGGAT 
ACGTTGGTGA TGAGTGGTAA TGGGAGGATG GCCTATGGGC ATGATAGGAC GGAGAGTGTG 
GTTAGTACTA TGACTGTGGG GACGATTGGT GGTGGTGGTG GTGGTGGTGG TTATCGGGAG 
AGGGAAAGGG AGAGGGAGGG AGAAAGAGAA GGGGAGGGAG AAGGGGAGGA TATGGCGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07658 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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