TurboCHO^™
or Nothing

Dr. Gabriele Casirati
Boston Children's Hospital

"Excellent quality and fast services, with a vast offer of molecular biology and protein/antibody products. The technical support is particularly helpful in finding the best solution for the specific experimental needs."

Ron Deckelbaum
Regeneron Pharmaceuticals Inc.

"We tasked the GenScript team with challenging projects for the production of engineered proteins that were novel and not necessarily straightforward. The GenScript team demonstrated a high level of commitment both timelines and quality product delivery."

David G.Harrison
Vanderbilt University

"GenScript has been very helpful for years. I consider them part of my lab. Rather than spending weeks and months expressing proteins, I get turn around on orders in a few weeks, and am never disappointed."

Victoria McGilligan
Ulster University

"Over the past few years, GenScript has become an indispensable partner in our research endeavors. Moreover, their custom protein services have been a game-changer. The purity and functionality of the proteins we’ve ordered have always met, if not exceeded, our expectations."

FAQ

• What’s the typical yield and purity?

  TurboCHO™ delivers high yield and purity across a wide range of antibody formats:

  Small Scale (1–30 mL)	Large Scale ( >100 mL)
  Avg yield > 200 mg/L Deliverable purity > 90%	Avg yield 400–600 mg/L with TurboCHO™ 2.0 platform1 Avg yield 600–1200 mg/L with TurboCHO™ 2.5 platform2 Deliverable purity > 95%

  Note:
  1. Data base on 5 day expression.
  2. Data base on 7 day expression

  TurboCHO™ Avg Yield (1-30mL)

  

  TurboCHO™ Avg Yield (>100 mL)

  

  

  TurboCHO™ Avg Purity (1-30mL)

  

  TurboCHO™ Avg Purity (>100 mL)

  

  
• What is GenScript's expertise in bispecific antibody production?

  Absolutely— TurboCHO™ was built for BsAb challenges.

  Our Proven Record	How we achieve it
  200+ BsAb formats successfully expressed 130,000+ BsAb projects completed Titer up to 2.4 g/L with TurboCHO™	Chain ratio optimization Design of experiment (DOE) Format-specific production insight, built on 130,000+ BsAb projects —see how our data can guide your format selection

  Explore how we solve BsAb challenges in real world cases:

  Case 1: Chain Ration Optimization

  Challenge: BsAb1 different chains exhibit varying expression levels, leading to low yield and purity when transfected with an equal molar plasmid ratio.

  Solution: Optimize the plasmid ratio during transfection. A chain ratio of 2:2:5 provides optimal purity and yield.

  

  

  

  Case 2: Purification Strategy Optimization

  Challenge: Diverse BsAb structures often lead to non-target species and low recovery, making purification difficult and yield limited.

  Solution: Drawing on experience from 13,000+ BsAb projects, GenScript’s PhD team developed a Purification Roadmap with format-specific strategies to improve purity and recovery.

  • Non IgG-like BsAb & Symmetric IgG-like BsAb

    • AC+SEC or AC+IEX for aggregate removal

  • Asymmetric IgG-like BsAb

    • Protein A base resin for capture chromatography
    • AC, SEC, IEX, HIC, MMC for polish chromatography based on the differences between POI and major impurities

  Case 3: BsAb Expression Titers Reaching Up to 2.4 g/L

  
• Does Genscript provide protein analytical services?

  Yes, GenScript’s Comprehensive Protein Analysis Platform offer comprehensive protein analysis:

  

  Qualitative & Quantitative Analysis

  • Concentration
  • Purity (SDS-PAGE, CE-SDS, icIEF, SEC-HPLC, RP-HPLC, HIC-HPLC, etc.)
  • Intact mass /Native mass
  • (in gel) Peptide mapping
  • N/C terminal sequencing

  

  Protein Modification Analysis

  • PTM analysis
  • Disulfide bond mapping
  • N-glycan and O-glycan profiling
  • Sialic acid analysis

  

  Impurity Analysis

  • Endotoxin quantification
  • HCP identification /quantification
  • Residual DNA quantification
  • Residual Protein A quantification

  

  Molecular Interaction Analysis

  • Antibody-antigen affinity analysis >>
  • Protein-protein/DNA /small molecule interactions
  • Fc effector function
  • Enzyme activity assay

  

  Customized Analysis

  • Monoclonal antibody self-interactions
  • Monoclonal antibody polyspecificity
  • Thermal stability analysis
  • Mutation site analysis
  • Free-thaw stability analysis
  • Antibody de novo sequencing
  • More customized services available