arfgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa60863 XM_012965257.3
Latest version!
Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa60864 XM_012965258.3
Latest version!
Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35255 XM_012965257.2
Latest version!
Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa35256 XM_012965258.2
Latest version!
Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa60863
    Clone ID Related Accession (Same CDS sequence) XM_012965257.3
    Accession Version XM_012965257.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT
    AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG
    TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG
    GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT
    AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA
    GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA
    AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC
    ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA
    AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG
    AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA
    GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA
    GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT
    CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC
    AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC
    TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG
    GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA
    AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT
    GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA
    GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA
    GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA
    GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA
    GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG
    TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC
    AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC
    TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT
    ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA
    GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG
    GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG
    ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA
    TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG
    CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT
    GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC
    CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG
    CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT
    CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT
    CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA
    GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA
    AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT
    GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG
    TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC
    CACTTTAATA AGGTTGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT
    CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG
    AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA
    GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA
    GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA
    GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC
    CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT
    GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT
    GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA
    GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC
    AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA
    ACCTATGGGC ACACATTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG
    ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT
    ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG
    AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT
    GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG
    AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC
    ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA
    CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT
    GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA
    GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA
    TTTGCAGCCT TGCTGATTAA ATGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC
    ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCAG AGAATCTGGC TGCGGCACAG
    AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC
    CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA
    AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG
    AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT
    CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA
    TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC
    AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGTGAT
    GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT
    GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT
    TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA
    CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820711.2
    CDS297..5876
    Translation

    Target ORF information:

    RefSeq Version XM_012965257.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965257.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT
    AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG
    TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG
    GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT
    AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA
    GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA
    AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC
    ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA
    AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG
    AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA
    GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA
    GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT
    CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC
    AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC
    TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG
    GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA
    AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT
    GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA
    GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA
    GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA
    GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA
    GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG
    TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC
    AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC
    TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT
    ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA
    GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG
    GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG
    ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA
    TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG
    CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT
    GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC
    CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG
    CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT
    CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT
    CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA
    GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA
    AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT
    GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG
    TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC
    CACTTTAATA AGGTTGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT
    CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG
    AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA
    GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA
    GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA
    GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC
    CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT
    GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT
    GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA
    GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC
    AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA
    ACCTATGGGC ACACATTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG
    ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT
    ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG
    AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT
    GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG
    AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC
    ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA
    CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT
    GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA
    GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA
    TTTGCAGCCT TGCTGATTAA ATGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC
    ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCAG AGAATCTGGC TGCGGCACAG
    AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC
    CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA
    AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG
    AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT
    CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA
    TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC
    AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGTGAT
    GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT
    GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT
    TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA
    CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60864
    Clone ID Related Accession (Same CDS sequence) XM_012965258.3
    Accession Version XM_012965258.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC
    CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG
    CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA
    CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG
    TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT
    GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG
    TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA
    ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT
    GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC
    AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA
    GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG
    GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT
    CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA
    GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT
    GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG
    CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA
    ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT
    TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC
    AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC
    TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA
    ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG
    CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG
    GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT
    AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA
    ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA
    TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA
    CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC
    ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC
    CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC
    CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA
    GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT
    GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT
    GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT
    GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA
    GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA
    GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA
    AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT
    GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA
    TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC
    ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT
    AAGGTTGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA
    CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT
    TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG
    GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG
    AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT
    GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA
    ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC
    CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT
    AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT
    GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC
    AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG
    CACACATTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT
    AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT
    CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG
    CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT
    GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC
    TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT
    CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC
    GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT
    CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA
    GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC
    TTGCTGATTA AATGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC
    TTCCCTGCAA CCAGTAGAAA AGAAGATGCA GAGAATCTGG CTGCGGCACA GAGGGACACG
    GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC
    CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT
    AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA
    CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT
    ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT
    GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC
    TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGTGA TGAACGGTTC
    AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC
    CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC
    TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC
    CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820712.2
    CDS296..5866
    Translation

    Target ORF information:

    RefSeq Version XM_012965258.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965258.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC
    CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG
    CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA
    CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG
    TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT
    GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG
    TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA
    ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT
    GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC
    AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA
    GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG
    GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT
    CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA
    GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT
    GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG
    CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA
    ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT
    TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC
    AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC
    TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA
    ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG
    CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG
    GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT
    AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA
    ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA
    TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA
    CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC
    ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC
    CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC
    CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA
    GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT
    GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT
    GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT
    GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA
    GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA
    GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA
    AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT
    GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA
    TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC
    ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT
    AAGGTTGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA
    CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT
    TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG
    GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG
    AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT
    GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA
    ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC
    CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT
    AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT
    GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC
    AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG
    CACACATTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT
    AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT
    CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG
    CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT
    GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC
    TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT
    CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC
    GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT
    CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA
    GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC
    TTGCTGATTA AATGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC
    TTCCCTGCAA CCAGTAGAAA AGAAGATGCA GAGAATCTGG CTGCGGCACA GAGGGACACG
    GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC
    CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT
    AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA
    CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT
    ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT
    GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC
    TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGTGA TGAACGGTTC
    AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC
    CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC
    TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC
    CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35255
    Clone ID Related Accession (Same CDS sequence) XM_012965257.2
    Accession Version XM_012965257.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT
    AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG
    TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG
    GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT
    AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA
    GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA
    AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC
    ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA
    AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG
    AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA
    GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA
    GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT
    CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC
    AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC
    TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG
    GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA
    AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT
    GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA
    GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA
    GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA
    GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA
    GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG
    TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC
    AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC
    TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT
    ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA
    GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG
    GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG
    ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA
    TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG
    CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT
    GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC
    CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG
    CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT
    CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT
    CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA
    GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA
    AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT
    GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG
    TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC
    CACTTTAATA AGGTCGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT
    CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG
    AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA
    GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA
    GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA
    GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC
    CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT
    GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT
    GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA
    GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC
    AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA
    ACCTATGGGC ACACGTTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG
    ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT
    ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG
    AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT
    GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG
    AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC
    ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA
    CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT
    GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA
    GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA
    TTTGCAGCCT TGCTGATTAA GTGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC
    ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCGG AGAATCTGGC TGCGGCACAG
    AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC
    CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA
    AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG
    AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT
    CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA
    TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC
    AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGCGAT
    GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT
    GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT
    TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA
    CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820711.1
    CDS331..5910
    Translation

    Target ORF information:

    RefSeq Version XM_012965257.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965257.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT
    AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG
    TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG
    GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT
    AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA
    GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA
    AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC
    ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA
    AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG
    AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA
    GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA
    GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT
    CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC
    AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC
    TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG
    GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA
    AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT
    GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA
    GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA
    GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA
    GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA
    GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG
    TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC
    AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC
    TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT
    ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA
    GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG
    GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG
    ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA
    TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG
    CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT
    GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC
    CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG
    CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT
    CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT
    CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA
    GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA
    AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT
    GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG
    TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC
    CACTTTAATA AGGTCGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT
    CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG
    AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA
    GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA
    GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA
    GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC
    CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT
    GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT
    GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA
    GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC
    AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA
    ACCTATGGGC ACACGTTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG
    ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT
    ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG
    AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT
    GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG
    AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC
    ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA
    CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT
    GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA
    GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA
    TTTGCAGCCT TGCTGATTAA GTGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC
    ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCGG AGAATCTGGC TGCGGCACAG
    AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC
    CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA
    AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG
    AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT
    CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA
    TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC
    AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGCGAT
    GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT
    GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT
    TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA
    CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35256
    Clone ID Related Accession (Same CDS sequence) XM_012965258.2
    Accession Version XM_012965258.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC
    CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG
    CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA
    CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG
    TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT
    GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG
    TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA
    ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT
    GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC
    AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA
    GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG
    GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT
    CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA
    GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT
    GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG
    CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA
    ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT
    TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC
    AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC
    TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA
    ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG
    CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG
    GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT
    AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA
    ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA
    TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA
    CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC
    ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC
    CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC
    CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA
    GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT
    GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT
    GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT
    GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA
    GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA
    GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA
    AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT
    GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA
    TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC
    ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT
    AAGGTCGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA
    CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT
    TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG
    GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG
    AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT
    GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA
    ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC
    CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT
    AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT
    GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC
    AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG
    CACACGTTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT
    AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT
    CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG
    CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT
    GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC
    TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT
    CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC
    GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT
    CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA
    GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC
    TTGCTGATTA AGTGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC
    TTCCCTGCAA CCAGTAGAAA AGAAGATGCG GAGAATCTGG CTGCGGCACA GAGGGACACG
    GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC
    CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT
    AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA
    CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT
    ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT
    GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC
    TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGCGA TGAACGGTTC
    AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC
    CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC
    TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC
    CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820712.1
    CDS331..5901
    Translation

    Target ORF information:

    RefSeq Version XM_012965258.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965258.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
    CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA
    AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA
    TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG
    TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT
    GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA
    ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA
    CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC
    ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC
    AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG
    GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC
    CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT
    CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC
    TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT
    GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG
    GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC
    ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT
    ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT
    GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC
    TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC
    CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG
    CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA
    CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG
    TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT
    GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG
    TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA
    ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT
    GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC
    AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA
    GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG
    GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT
    CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA
    GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT
    GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG
    CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA
    ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT
    TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC
    AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC
    TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA
    ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG
    CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG
    GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT
    AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA
    ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA
    TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA
    CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC
    ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC
    CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC
    CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA
    GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT
    GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT
    GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT
    GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA
    GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA
    GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA
    AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT
    GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA
    TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC
    ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT
    AAGGTCGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA
    CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT
    TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG
    GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG
    AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT
    GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA
    ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC
    CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT
    AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT
    GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC
    AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG
    CACACGTTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT
    AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT
    CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG
    CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT
    GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC
    TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT
    CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC
    GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT
    CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA
    GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC
    TTGCTGATTA AGTGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC
    TTCCCTGCAA CCAGTAGAAA AGAAGATGCG GAGAATCTGG CTGCGGCACA GAGGGACACG
    GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC
    CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT
    AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA
    CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT
    ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT
    GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC
    TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGCGA TGAACGGTTC
    AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC
    CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC
    TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC
    CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.