agef-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	agef-1
Entrez Gene ID	173231
Full Name	Arf-1 Guanine nucleotide Exchange Factor homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001026627.5	NP_001021798.2	Arf-1 Guanine nucleotide Exchange Factor homolog
NM_001026628.3	NP_001021799.1	Arf-1 Guanine nucleotide Exchange Factor homolog
NM_001026627.6	NP_001021798.2	SEC7 domain-containing protein
NM_001026628.4	NP_001021799.1	SEC7 domain-containing protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An AGEF-1/Arf GTPase/AP-1 ensemble antagonizes LET-23 basolateral localization and signaling during C. elegans vulva induction.
Title: An AGEF-1/Arf GTPase/AP-1 ensemble antagonizes LET-23 EGFR basolateral localization and signaling during C. elegans vulva induction.

The following agef-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agef-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336271	NM_001026628.4 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (agef-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa335583	NM_001026627.6 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (agef-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa335583	NM_001026627.5	Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa336271	NM_001026628.3	Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agef-1 ( NM_001026628.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021799.1 Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.

Price & Availability↑

CloneID	OCa336271
Clone ID Related Accession (Same CDS sequence)	NM_001026628.3 , NM_001026628.4
Accession Version	NM_001026628.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Arf-1 Guanine nucleotide Exchange Factor homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026628.2. COMPLETENESS: incomplete on both ends.

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGGTACG CGGATTGATT TGAAACGACG GCGATTTTTT 
TATGGAAATA TTGGGAAAAA TCCCGAAAAA CGAACATTTA TAATCAATTT TCACGTTAAA 
ATACTAGAAA TTGAAATTAA AATTGACGAA AATCGCGAAT TCCGAGTTTC CAAAAACTGC 
GTTCCGACCA GCGTTCGCGA CCACGCCTAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021799.1
CDS	1..1656
Translation	MSELSNGPKKSIGNMFLRSGIEKILADRDIKRKENLQLKKACESALEELKAAEEQDASPSSNGEHLPDAGGTAVEADRYFLPFELACNSKSPKIVITALDCLQKLIAYGHLTGRGADISNPERKLIDRIVEAICAPFLGQGTDETVLLQLIKAVLAVVLSTHCEVHGASLILAVRTCFNIYLTSKSPINQATAKGTLTQVINTVFGNMEKFGNIKDDETIVREVVEVLVSNTISNEVSDETSEAGGTHRQNGSTMGESEAPLDDQFTFMNAYQKDAFLVFRALCILAQKEEGGASNEMSLRSKLLALEMLLLVLQNSSSILQSSQPCIIVIKRTLCMALTRNAVSNNIQVFEKSLAIFVELLDKFKTHLKASIEVFFNSVILPMLDSNTCAFEQKWIVLNTIGKILANPQSVVDMFVNYDCDMTSPNLFKSIVEVVSKTTRTTINENAPPAQKEKERAMRLLGLSCLTDLLQCLVDWWQVCEVQKITSGTRIDLKRRRFFYGNIGKNPEKRTFIINFHVKILEIEIKIDENREFRVSKNCVPTSVRDHAYF

Target ORF information:

RefSeq Version	NM_001026628.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026628.3

ORF Insert Sequence:

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGGTACG CGGATTGATT TGAAACGACG GCGATTTTTT 
TATGGAAATA TTGGGAAAAA TCCCGAAAAA CGAACATTTA TAATCAATTT TCACGTTAAA 
ATACTAGAAA TTGAAATTAA AATTGACGAA AATCGCGAAT TCCGAGTTTC CAAAAACTGC 
GTTCCGACCA GCGTTCGCGA CCACGCCTAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agef-1 ( NM_001026628.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021799.1 Caenorhabditis elegans SEC7 domain-containing protein (agef-1), partial mRNA.

Price & Availability↑

CloneID	OCa336271
Clone ID Related Accession (Same CDS sequence)	NM_001026628.3 , NM_001026628.4
Accession Version	NM_001026628.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026628.3. COMPLETENESS: incomplete on the 3' end.

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGGTACG CGGATTGATT TGAAACGACG GCGATTTTTT 
TATGGAAATA TTGGGAAAAA TCCCGAAAAA CGAACATTTA TAATCAATTT TCACGTTAAA 
ATACTAGAAA TTGAAATTAA AATTGACGAA AATCGCGAAT TCCGAGTTTC CAAAAACTGC 
GTTCCGACCA GCGTTCGCGA CCACGCCTAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021799.1
CDS	28..1683
Translation	MSELSNGPKKSIGNMFLRSGIEKILADRDIKRKENLQLKKACESALEELKAAEEQDASPSSNGEHLPDAGGTAVEADRYFLPFELACNSKSPKIVITALDCLQKLIAYGHLTGRGADISNPERKLIDRIVEAICAPFLGQGTDETVLLQLIKAVLAVVLSTHCEVHGASLILAVRTCFNIYLTSKSPINQATAKGTLTQVINTVFGNMEKFGNIKDDETIVREVVEVLVSNTISNEVSDETSEAGGTHRQNGSTMGESEAPLDDQFTFMNAYQKDAFLVFRALCILAQKEEGGASNEMSLRSKLLALEMLLLVLQNSSSILQSSQPCIIVIKRTLCMALTRNAVSNNIQVFEKSLAIFVELLDKFKTHLKASIEVFFNSVILPMLDSNTCAFEQKWIVLNTIGKILANPQSVVDMFVNYDCDMTSPNLFKSIVEVVSKTTRTTINENAPPAQKEKERAMRLLGLSCLTDLLQCLVDWWQVCEVQKITSGTRIDLKRRRFFYGNIGKNPEKRTFIINFHVKILEIEIKIDENREFRVSKNCVPTSVRDHAYF

Target ORF information:

RefSeq Version	NM_001026628.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (agef-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026628.4

ORF Insert Sequence:

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGGTACG CGGATTGATT TGAAACGACG GCGATTTTTT 
TATGGAAATA TTGGGAAAAA TCCCGAAAAA CGAACATTTA TAATCAATTT TCACGTTAAA 
ATACTAGAAA TTGAAATTAA AATTGACGAA AATCGCGAAT TCCGAGTTTC CAAAAACTGC 
GTTCCGACCA GCGTTCGCGA CCACGCCTAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agef-1 ( NM_001026627.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021798.2 Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.

Price & Availability↑

CloneID	OCa335583
Clone ID Related Accession (Same CDS sequence)	NM_001026627.5 , NM_001026627.6
Accession Version	NM_001026627.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Arf-1 Guanine nucleotide Exchange Factor homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026627.4. COMPLETENESS: incomplete on both ends.

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGATATC GATGACGCGG AGCCCAGTGA GCAACAACAC 
GGTGAAACAT TCGAAGCATT TGAAACTCTA AAACAACAAA AAAATCTAAT GGAACAAGGC 
ATTCAAATAT TCTCGGAAAA ACCGAAAAAA GGACTGAAAT TCCTTCAAGA ACACGGTTTT 
GTTGGAACTG ATGCTGTAGA AGTTGCCGAG TTTATGATGA AAGAAGAACG GCTGAATAAG 
ACACAAGTTG GAGATTTTCT AGGCGATTCT GATGAATTTA ATAATTCTGT AATGCATGCA 
TATATTGATT TTTTGGATTT CTCGTCGATT GATATTCTCG CAGCGTTGAG AATGTTTTTG 
GAGAAATTTA GATTACCAGG AGAAGCTCAA AAAATCGATC GCCTAATGTT GAAATTCGCT 
TCGCGATACC TGGACTGTAA TCCACGCCAA GGAATATTTG CCTCAGCCGA CGCTGCCTAC 
GTACTCGCGT TCTCAATTAT CATGTTAACA ACGGATCTAC ATAATAAAAC AGTAAAGAAT 
AAAATGACGA AACAAGGATA TATTAATATG AATCGAGGTA TTAATGAAGG TGGAAATATT 
CCTGTTGAAC TGCTCGAAGC GATTTTCGAA GATATTTCGA AGAATGAGAT TAAGATGAGA 
GCCGGAGCCA CTGCTTTGCT CAGATCTCGG GTTACACCAG GACAAGGAGC ACTGGCAACT 
GATAAAGAAC GAAGAGCTAT GGCTGCACTG GAAATGGAGG CTTTGTCTGA AACTGCGAGA 
GCCCTAATGG AGTCTGCAAG TGACGCTGAT GCCTATTTCA CTCCAGCACA ACACCAACAT 
CACGTAAAAC CAATGTTCAA AATCTGCTGG ACACCATGCC TTGCCGCATT CTCAGTCGGT 
GTACAAATGT CGGATGATGA AGAAGAATGG TCTCTTTGTT TGAGAGGTTT TCGTCTGGGA 
GTACGTGCCG CGTGTGTTCT ACAAGCAACA CTTGAACGGA ATGCATTTAT ACAGGCACTT 
GCGAGATTTA CTCTTTTAAC GGCAAAAAAT TCATTGGGAG AAATGCGAGT GAAGAATATT 
GAAGCAATCA AGCTTTTACT ATTAATTGGT GATGAAGATG GAGAATATCT TGAAGAGAAT 
TGGGTTGATG TTATGAAATG TATGAGTTCT CTTGAATTAG TACAATTAAT TGGAACTGGA 
CTTAATTCTG CAATGTCTCA TGATACGGAT AGTAGTCGAC AGTACGTGAT GAAAGCCACC 
GGAGGAATCG ATGAAAAAAC GCTTCACTCT TTGCAAGATG CTCTCGGTGA AACATCATCT 
CAATCAGTTG TAGTGGCAAT TGATCGAATA TTCAATGGTT CCGCCCGTCT TTCAGCAGAA 
GCTATTGTAT ATTTTGTACG TGCTCTTTGT GCAGTCTCTC GAGAAGAGCT ATCACATCCG 
GCGGCTCCAC GAATGTTTTT ATTGGGAAAA GTTGTTGAAG TGGCGTTCTA TAATATGAAT 
AGAATTCGTT TGGAATGGAG TCGAATTTGG AATGTTATCG GTGAACATTT TAATGCGGCT 
GGATGTAATT CAAATGAAGC TGTTGCCTAT TTTTCGGTGG ATGCGCTGAG GCAGTTGTCG 
ATCAAATTTT TGGAAAAGGG AGAACTTCCG AATTTCCGAT TTCAGAAGGA TTTCTTACGG 
CCGTTTGAGG TAATAATGGT TCGAAACGGA AGTGCCCAGA CACGAGATCT TGTCGTTCGA 
TGCTGTGCTC ATTTAGTAGA AGCTCACTCG TCTCGACTCA AATCCGGATG GCAGAATCTC 
TTTTCAGTAT GGACAATTGC CGCCGGAGAC CCTTCTACTG AAATTGGTGA AGCATCATTC 
CTCACCGCTC AGAAAGTCAT CGAAAAACGT TTTAAAGAGG ATTTTCCGGC GTTTTTAGAC 
TCATTTCAAG AGGCGTTGAA GTGTTTACAA GAGTTTGCAT GTAATCAAAA TCAACCTGAT 
ATGAATATGG AAGCAATTCG ATTGATTCGA CTTTGTGCTG ACTACGTATC GGAGAATAGT 
GACAAAATCG ATGAGGCCGC CCGTCGTGAT GATCATCTTC ATAAGGGATT AACTGCTGAT 
CAACACGTAT GGCTACGTGG ATGGTTTCCA ATATTCTTTG AATTGAGTTG TATTATTAAT 
CGATGCAAAT TGGATGTTCG AACACGGAGC TTAACTGTCA TGTTTGAAAT TATGAAACAT 
CATGGATCGG ATTTTAGGCC AGAATGGTGG AAGGATCTGT TGGAAATCGT TTTTCGCATT 
TTTGATCCAT CGAAAATGGA CGATCATCGA AGTGATAAGC GAGAATGGAT GTCAACGACG 
TGTAATCATG CAATGTTGAG TGTTGTCGAA GTGTTCACAC AATTCTACAC TCAACTCTCC 
GTCTACGCAC TTCCAATGAT CTATCGTCAA TTTGGAATCT TTATTCGACA ACAAAACGAA 
CAGCTCGCCA GATGTACGAT TAGTTGCCTG GAATCACTAA TCTCACAGAA CGGAGAACGA 
TTCACTGAGC CAATGTGGGA ACAGACTATT GAATTGATTC GGGAGCTATT TGAGACGACA 
CTGCCGAAAT CGTTGCTCAC ATGGGAGCCG CCGAATTCTA ATGGAATTGG GTCGGAGGAT 
CGGACGAATG GATCCGACAC TCTCTCATCG GAACAAATCG TCTTCTGTGT TGTACAAAAT 
GAGCTCGTCG AAGCGGTCTC CCGGATTGTG CTCGGAGACC ATCGGGAAAG TAAACGAGAT 
CTTCAAATCG ATGGCCTATT CACTCAAATG TCACCACAAT TACTTTTGAG CATTTGTGAT 
GCTCTCGCGG AAAGTCATAC ACTGGCGAAA CAATTCAATA ATAATAATGG GCAACGTGTA 
TTGATTTGGA AAGCACGACT ACTTGGATCT ACGAAGCCCA ATTTGATCAA CCAAGAAACG 
CGATCGCTAA GTGCAATGTT GGCTATTCTA TTCCGCCTGC TCTACGATCT CCGAGCCCAA 
TCGATTTCTG ACAAAATTGC GATTCGAGTG TTAGAAGTCG TTTCACTTGC GCTCAGCGGC 
TACGGAGAAG CCGAATCCGA CACCAGGCGG ACTGCGTATG GACCAGTAAT TTGTGAATTG 
TTGAGAGAAT GCATTGCACT TCCGACCAAC TTGATTCCGG TACTTGGGCC GGAGTTTCCA 
TTAAAACTCT GCGATCTCGT TGAAACGGCC GAAGATCAAA CGATGCGAAA ACTGCTCGCC 
GAGCTCTTCC GACGAATAAT CAATTTTCAA ACGAAATCAC CAACAACTGT TCAAGAAGCG 
CTAGAAGAAT CTCAGAAATC CCATATTTCG GAAAATTTCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021798.2
CDS	1..4785
Translation	MSELSNGPKKSIGNMFLRSGIEKILADRDIKRKENLQLKKACESALEELKAAEEQDASPSSNGEHLPDAGGTAVEADRYFLPFELACNSKSPKIVITALDCLQKLIAYGHLTGRGADISNPERKLIDRIVEAICAPFLGQGTDETVLLQLIKAVLAVVLSTHCEVHGASLILAVRTCFNIYLTSKSPINQATAKGTLTQVINTVFGNMEKFGNIKDDETIVREVVEVLVSNTISNEVSDETSEAGGTHRQNGSTMGESEAPLDDQFTFMNAYQKDAFLVFRALCILAQKEEGGASNEMSLRSKLLALEMLLLVLQNSSSILQSSQPCIIVIKRTLCMALTRNAVSNNIQVFEKSLAIFVELLDKFKTHLKASIEVFFNSVILPMLDSNTCAFEQKWIVLNTIGKILANPQSVVDMFVNYDCDMTSPNLFKSIVEVVSKTTRTTINENAPPAQKEKERAMRLLGLSCLTDLLQCLVDWWQVCEVQKITSDIDDAEPSEQQHGETFEAFETLKQQKNLMEQGIQIFSEKPKKGLKFLQEHGFVGTDAVEVAEFMMKEERLNKTQVGDFLGDSDEFNNSVMHAYIDFLDFSSIDILAALRMFLEKFRLPGEAQKIDRLMLKFASRYLDCNPRQGIFASADAAYVLAFSIIMLTTDLHNKTVKNKMTKQGYINMNRGINEGGNIPVELLEAIFEDISKNEIKMRAGATALLRSRVTPGQGALATDKERRAMAALEMEALSETARALMESASDADAYFTPAQHQHHVKPMFKICWTPCLAAFSVGVQMSDDEEEWSLCLRGFRLGVRAACVLQATLERNAFIQALARFTLLTAKNSLGEMRVKNIEAIKLLLLIGDEDGEYLEENWVDVMKCMSSLELVQLIGTGLNSAMSHDTDSSRQYVMKATGGIDEKTLHSLQDALGETSSQSVVVAIDRIFNGSARLSAEAIVYFVRALCAVSREELSHPAAPRMFLLGKVVEVAFYNMNRIRLEWSRIWNVIGEHFNAAGCNSNEAVAYFSVDALRQLSIKFLEKGELPNFRFQKDFLRPFEVIMVRNGSAQTRDLVVRCCAHLVEAHSSRLKSGWQNLFSVWTIAAGDPSTEIGEASFLTAQKVIEKRFKEDFPAFLDSFQEALKCLQEFACNQNQPDMNMEAIRLIRLCADYVSENSDKIDEAARRDDHLHKGLTADQHVWLRGWFPIFFELSCIINRCKLDVRTRSLTVMFEIMKHHGSDFRPEWWKDLLEIVFRIFDPSKMDDHRSDKREWMSTTCNHAMLSVVEVFTQFYTQLSVYALPMIYRQFGIFIRQQNEQLARCTISCLESLISQNGERFTEPMWEQTIELIRELFETTLPKSLLTWEPPNSNGIGSEDRTNGSDTLSSEQIVFCVVQNELVEAVSRIVLGDHRESKRDLQIDGLFTQMSPQLLLSICDALAESHTLAKQFNNNNGQRVLIWKARLLGSTKPNLINQETRSLSAMLAILFRLLYDLRAQSISDKIAIRVLEVVSLALSGYGEAESDTRRTAYGPVICELLRECIALPTNLIPVLGPEFPLKLCDLVETAEDQTMRKLLAELFRRIINFQTKSPTTVQEALEESQKSHISENFT

Target ORF information:

RefSeq Version	NM_001026627.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Arf-1 Guanine nucleotide Exchange Factor homolog (agef-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026627.5

ORF Insert Sequence:

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGATATC GATGACGCGG AGCCCAGTGA GCAACAACAC 
GGTGAAACAT TCGAAGCATT TGAAACTCTA AAACAACAAA AAAATCTAAT GGAACAAGGC 
ATTCAAATAT TCTCGGAAAA ACCGAAAAAA GGACTGAAAT TCCTTCAAGA ACACGGTTTT 
GTTGGAACTG ATGCTGTAGA AGTTGCCGAG TTTATGATGA AAGAAGAACG GCTGAATAAG 
ACACAAGTTG GAGATTTTCT AGGCGATTCT GATGAATTTA ATAATTCTGT AATGCATGCA 
TATATTGATT TTTTGGATTT CTCGTCGATT GATATTCTCG CAGCGTTGAG AATGTTTTTG 
GAGAAATTTA GATTACCAGG AGAAGCTCAA AAAATCGATC GCCTAATGTT GAAATTCGCT 
TCGCGATACC TGGACTGTAA TCCACGCCAA GGAATATTTG CCTCAGCCGA CGCTGCCTAC 
GTACTCGCGT TCTCAATTAT CATGTTAACA ACGGATCTAC ATAATAAAAC AGTAAAGAAT 
AAAATGACGA AACAAGGATA TATTAATATG AATCGAGGTA TTAATGAAGG TGGAAATATT 
CCTGTTGAAC TGCTCGAAGC GATTTTCGAA GATATTTCGA AGAATGAGAT TAAGATGAGA 
GCCGGAGCCA CTGCTTTGCT CAGATCTCGG GTTACACCAG GACAAGGAGC ACTGGCAACT 
GATAAAGAAC GAAGAGCTAT GGCTGCACTG GAAATGGAGG CTTTGTCTGA AACTGCGAGA 
GCCCTAATGG AGTCTGCAAG TGACGCTGAT GCCTATTTCA CTCCAGCACA ACACCAACAT 
CACGTAAAAC CAATGTTCAA AATCTGCTGG ACACCATGCC TTGCCGCATT CTCAGTCGGT 
GTACAAATGT CGGATGATGA AGAAGAATGG TCTCTTTGTT TGAGAGGTTT TCGTCTGGGA 
GTACGTGCCG CGTGTGTTCT ACAAGCAACA CTTGAACGGA ATGCATTTAT ACAGGCACTT 
GCGAGATTTA CTCTTTTAAC GGCAAAAAAT TCATTGGGAG AAATGCGAGT GAAGAATATT 
GAAGCAATCA AGCTTTTACT ATTAATTGGT GATGAAGATG GAGAATATCT TGAAGAGAAT 
TGGGTTGATG TTATGAAATG TATGAGTTCT CTTGAATTAG TACAATTAAT TGGAACTGGA 
CTTAATTCTG CAATGTCTCA TGATACGGAT AGTAGTCGAC AGTACGTGAT GAAAGCCACC 
GGAGGAATCG ATGAAAAAAC GCTTCACTCT TTGCAAGATG CTCTCGGTGA AACATCATCT 
CAATCAGTTG TAGTGGCAAT TGATCGAATA TTCAATGGTT CCGCCCGTCT TTCAGCAGAA 
GCTATTGTAT ATTTTGTACG TGCTCTTTGT GCAGTCTCTC GAGAAGAGCT ATCACATCCG 
GCGGCTCCAC GAATGTTTTT ATTGGGAAAA GTTGTTGAAG TGGCGTTCTA TAATATGAAT 
AGAATTCGTT TGGAATGGAG TCGAATTTGG AATGTTATCG GTGAACATTT TAATGCGGCT 
GGATGTAATT CAAATGAAGC TGTTGCCTAT TTTTCGGTGG ATGCGCTGAG GCAGTTGTCG 
ATCAAATTTT TGGAAAAGGG AGAACTTCCG AATTTCCGAT TTCAGAAGGA TTTCTTACGG 
CCGTTTGAGG TAATAATGGT TCGAAACGGA AGTGCCCAGA CACGAGATCT TGTCGTTCGA 
TGCTGTGCTC ATTTAGTAGA AGCTCACTCG TCTCGACTCA AATCCGGATG GCAGAATCTC 
TTTTCAGTAT GGACAATTGC CGCCGGAGAC CCTTCTACTG AAATTGGTGA AGCATCATTC 
CTCACCGCTC AGAAAGTCAT CGAAAAACGT TTTAAAGAGG ATTTTCCGGC GTTTTTAGAC 
TCATTTCAAG AGGCGTTGAA GTGTTTACAA GAGTTTGCAT GTAATCAAAA TCAACCTGAT 
ATGAATATGG AAGCAATTCG ATTGATTCGA CTTTGTGCTG ACTACGTATC GGAGAATAGT 
GACAAAATCG ATGAGGCCGC CCGTCGTGAT GATCATCTTC ATAAGGGATT AACTGCTGAT 
CAACACGTAT GGCTACGTGG ATGGTTTCCA ATATTCTTTG AATTGAGTTG TATTATTAAT 
CGATGCAAAT TGGATGTTCG AACACGGAGC TTAACTGTCA TGTTTGAAAT TATGAAACAT 
CATGGATCGG ATTTTAGGCC AGAATGGTGG AAGGATCTGT TGGAAATCGT TTTTCGCATT 
TTTGATCCAT CGAAAATGGA CGATCATCGA AGTGATAAGC GAGAATGGAT GTCAACGACG 
TGTAATCATG CAATGTTGAG TGTTGTCGAA GTGTTCACAC AATTCTACAC TCAACTCTCC 
GTCTACGCAC TTCCAATGAT CTATCGTCAA TTTGGAATCT TTATTCGACA ACAAAACGAA 
CAGCTCGCCA GATGTACGAT TAGTTGCCTG GAATCACTAA TCTCACAGAA CGGAGAACGA 
TTCACTGAGC CAATGTGGGA ACAGACTATT GAATTGATTC GGGAGCTATT TGAGACGACA 
CTGCCGAAAT CGTTGCTCAC ATGGGAGCCG CCGAATTCTA ATGGAATTGG GTCGGAGGAT 
CGGACGAATG GATCCGACAC TCTCTCATCG GAACAAATCG TCTTCTGTGT TGTACAAAAT 
GAGCTCGTCG AAGCGGTCTC CCGGATTGTG CTCGGAGACC ATCGGGAAAG TAAACGAGAT 
CTTCAAATCG ATGGCCTATT CACTCAAATG TCACCACAAT TACTTTTGAG CATTTGTGAT 
GCTCTCGCGG AAAGTCATAC ACTGGCGAAA CAATTCAATA ATAATAATGG GCAACGTGTA 
TTGATTTGGA AAGCACGACT ACTTGGATCT ACGAAGCCCA ATTTGATCAA CCAAGAAACG 
CGATCGCTAA GTGCAATGTT GGCTATTCTA TTCCGCCTGC TCTACGATCT CCGAGCCCAA 
TCGATTTCTG ACAAAATTGC GATTCGAGTG TTAGAAGTCG TTTCACTTGC GCTCAGCGGC 
TACGGAGAAG CCGAATCCGA CACCAGGCGG ACTGCGTATG GACCAGTAAT TTGTGAATTG 
TTGAGAGAAT GCATTGCACT TCCGACCAAC TTGATTCCGG TACTTGGGCC GGAGTTTCCA 
TTAAAACTCT GCGATCTCGT TGAAACGGCC GAAGATCAAA CGATGCGAAA ACTGCTCGCC 
GAGCTCTTCC GACGAATAAT CAATTTTCAA ACGAAATCAC CAACAACTGT TCAAGAAGCG 
CTAGAAGAAT CTCAGAAATC CCATATTTCG GAAAATTTCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agef-1 ( NM_001026627.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021798.2 Caenorhabditis elegans SEC7 domain-containing protein (agef-1), mRNA.

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CloneID	OCa335583
Clone ID Related Accession (Same CDS sequence)	NM_001026627.5 , NM_001026627.6
Accession Version	NM_001026627.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026627.5.

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGATATC GATGACGCGG AGCCCAGTGA GCAACAACAC 
GGTGAAACAT TCGAAGCATT TGAAACTCTA AAACAACAAA AAAATCTAAT GGAACAAGGC 
ATTCAAATAT TCTCGGAAAA ACCGAAAAAA GGACTGAAAT TCCTTCAAGA ACACGGTTTT 
GTTGGAACTG ATGCTGTAGA AGTTGCCGAG TTTATGATGA AAGAAGAACG GCTGAATAAG 
ACACAAGTTG GAGATTTTCT AGGCGATTCT GATGAATTTA ATAATTCTGT AATGCATGCA 
TATATTGATT TTTTGGATTT CTCGTCGATT GATATTCTCG CAGCGTTGAG AATGTTTTTG 
GAGAAATTTA GATTACCAGG AGAAGCTCAA AAAATCGATC GCCTAATGTT GAAATTCGCT 
TCGCGATACC TGGACTGTAA TCCACGCCAA GGAATATTTG CCTCAGCCGA CGCTGCCTAC 
GTACTCGCGT TCTCAATTAT CATGTTAACA ACGGATCTAC ATAATAAAAC AGTAAAGAAT 
AAAATGACGA AACAAGGATA TATTAATATG AATCGAGGTA TTAATGAAGG TGGAAATATT 
CCTGTTGAAC TGCTCGAAGC GATTTTCGAA GATATTTCGA AGAATGAGAT TAAGATGAGA 
GCCGGAGCCA CTGCTTTGCT CAGATCTCGG GTTACACCAG GACAAGGAGC ACTGGCAACT 
GATAAAGAAC GAAGAGCTAT GGCTGCACTG GAAATGGAGG CTTTGTCTGA AACTGCGAGA 
GCCCTAATGG AGTCTGCAAG TGACGCTGAT GCCTATTTCA CTCCAGCACA ACACCAACAT 
CACGTAAAAC CAATGTTCAA AATCTGCTGG ACACCATGCC TTGCCGCATT CTCAGTCGGT 
GTACAAATGT CGGATGATGA AGAAGAATGG TCTCTTTGTT TGAGAGGTTT TCGTCTGGGA 
GTACGTGCCG CGTGTGTTCT ACAAGCAACA CTTGAACGGA ATGCATTTAT ACAGGCACTT 
GCGAGATTTA CTCTTTTAAC GGCAAAAAAT TCATTGGGAG AAATGCGAGT GAAGAATATT 
GAAGCAATCA AGCTTTTACT ATTAATTGGT GATGAAGATG GAGAATATCT TGAAGAGAAT 
TGGGTTGATG TTATGAAATG TATGAGTTCT CTTGAATTAG TACAATTAAT TGGAACTGGA 
CTTAATTCTG CAATGTCTCA TGATACGGAT AGTAGTCGAC AGTACGTGAT GAAAGCCACC 
GGAGGAATCG ATGAAAAAAC GCTTCACTCT TTGCAAGATG CTCTCGGTGA AACATCATCT 
CAATCAGTTG TAGTGGCAAT TGATCGAATA TTCAATGGTT CCGCCCGTCT TTCAGCAGAA 
GCTATTGTAT ATTTTGTACG TGCTCTTTGT GCAGTCTCTC GAGAAGAGCT ATCACATCCG 
GCGGCTCCAC GAATGTTTTT ATTGGGAAAA GTTGTTGAAG TGGCGTTCTA TAATATGAAT 
AGAATTCGTT TGGAATGGAG TCGAATTTGG AATGTTATCG GTGAACATTT TAATGCGGCT 
GGATGTAATT CAAATGAAGC TGTTGCCTAT TTTTCGGTGG ATGCGCTGAG GCAGTTGTCG 
ATCAAATTTT TGGAAAAGGG AGAACTTCCG AATTTCCGAT TTCAGAAGGA TTTCTTACGG 
CCGTTTGAGG TAATAATGGT TCGAAACGGA AGTGCCCAGA CACGAGATCT TGTCGTTCGA 
TGCTGTGCTC ATTTAGTAGA AGCTCACTCG TCTCGACTCA AATCCGGATG GCAGAATCTC 
TTTTCAGTAT GGACAATTGC CGCCGGAGAC CCTTCTACTG AAATTGGTGA AGCATCATTC 
CTCACCGCTC AGAAAGTCAT CGAAAAACGT TTTAAAGAGG ATTTTCCGGC GTTTTTAGAC 
TCATTTCAAG AGGCGTTGAA GTGTTTACAA GAGTTTGCAT GTAATCAAAA TCAACCTGAT 
ATGAATATGG AAGCAATTCG ATTGATTCGA CTTTGTGCTG ACTACGTATC GGAGAATAGT 
GACAAAATCG ATGAGGCCGC CCGTCGTGAT GATCATCTTC ATAAGGGATT AACTGCTGAT 
CAACACGTAT GGCTACGTGG ATGGTTTCCA ATATTCTTTG AATTGAGTTG TATTATTAAT 
CGATGCAAAT TGGATGTTCG AACACGGAGC TTAACTGTCA TGTTTGAAAT TATGAAACAT 
CATGGATCGG ATTTTAGGCC AGAATGGTGG AAGGATCTGT TGGAAATCGT TTTTCGCATT 
TTTGATCCAT CGAAAATGGA CGATCATCGA AGTGATAAGC GAGAATGGAT GTCAACGACG 
TGTAATCATG CAATGTTGAG TGTTGTCGAA GTGTTCACAC AATTCTACAC TCAACTCTCC 
GTCTACGCAC TTCCAATGAT CTATCGTCAA TTTGGAATCT TTATTCGACA ACAAAACGAA 
CAGCTCGCCA GATGTACGAT TAGTTGCCTG GAATCACTAA TCTCACAGAA CGGAGAACGA 
TTCACTGAGC CAATGTGGGA ACAGACTATT GAATTGATTC GGGAGCTATT TGAGACGACA 
CTGCCGAAAT CGTTGCTCAC ATGGGAGCCG CCGAATTCTA ATGGAATTGG GTCGGAGGAT 
CGGACGAATG GATCCGACAC TCTCTCATCG GAACAAATCG TCTTCTGTGT TGTACAAAAT 
GAGCTCGTCG AAGCGGTCTC CCGGATTGTG CTCGGAGACC ATCGGGAAAG TAAACGAGAT 
CTTCAAATCG ATGGCCTATT CACTCAAATG TCACCACAAT TACTTTTGAG CATTTGTGAT 
GCTCTCGCGG AAAGTCATAC ACTGGCGAAA CAATTCAATA ATAATAATGG GCAACGTGTA 
TTGATTTGGA AAGCACGACT ACTTGGATCT ACGAAGCCCA ATTTGATCAA CCAAGAAACG 
CGATCGCTAA GTGCAATGTT GGCTATTCTA TTCCGCCTGC TCTACGATCT CCGAGCCCAA 
TCGATTTCTG ACAAAATTGC GATTCGAGTG TTAGAAGTCG TTTCACTTGC GCTCAGCGGC 
TACGGAGAAG CCGAATCCGA CACCAGGCGG ACTGCGTATG GACCAGTAAT TTGTGAATTG 
TTGAGAGAAT GCATTGCACT TCCGACCAAC TTGATTCCGG TACTTGGGCC GGAGTTTCCA 
TTAAAACTCT GCGATCTCGT TGAAACGGCC GAAGATCAAA CGATGCGAAA ACTGCTCGCC 
GAGCTCTTCC GACGAATAAT CAATTTTCAA ACGAAATCAC CAACAACTGT TCAAGAAGCG 
CTAGAAGAAT CTCAGAAATC CCATATTTCG GAAAATTTCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021798.2
CDS	28..4812
Translation	MSELSNGPKKSIGNMFLRSGIEKILADRDIKRKENLQLKKACESALEELKAAEEQDASPSSNGEHLPDAGGTAVEADRYFLPFELACNSKSPKIVITALDCLQKLIAYGHLTGRGADISNPERKLIDRIVEAICAPFLGQGTDETVLLQLIKAVLAVVLSTHCEVHGASLILAVRTCFNIYLTSKSPINQATAKGTLTQVINTVFGNMEKFGNIKDDETIVREVVEVLVSNTISNEVSDETSEAGGTHRQNGSTMGESEAPLDDQFTFMNAYQKDAFLVFRALCILAQKEEGGASNEMSLRSKLLALEMLLLVLQNSSSILQSSQPCIIVIKRTLCMALTRNAVSNNIQVFEKSLAIFVELLDKFKTHLKASIEVFFNSVILPMLDSNTCAFEQKWIVLNTIGKILANPQSVVDMFVNYDCDMTSPNLFKSIVEVVSKTTRTTINENAPPAQKEKERAMRLLGLSCLTDLLQCLVDWWQVCEVQKITSDIDDAEPSEQQHGETFEAFETLKQQKNLMEQGIQIFSEKPKKGLKFLQEHGFVGTDAVEVAEFMMKEERLNKTQVGDFLGDSDEFNNSVMHAYIDFLDFSSIDILAALRMFLEKFRLPGEAQKIDRLMLKFASRYLDCNPRQGIFASADAAYVLAFSIIMLTTDLHNKTVKNKMTKQGYINMNRGINEGGNIPVELLEAIFEDISKNEIKMRAGATALLRSRVTPGQGALATDKERRAMAALEMEALSETARALMESASDADAYFTPAQHQHHVKPMFKICWTPCLAAFSVGVQMSDDEEEWSLCLRGFRLGVRAACVLQATLERNAFIQALARFTLLTAKNSLGEMRVKNIEAIKLLLLIGDEDGEYLEENWVDVMKCMSSLELVQLIGTGLNSAMSHDTDSSRQYVMKATGGIDEKTLHSLQDALGETSSQSVVVAIDRIFNGSARLSAEAIVYFVRALCAVSREELSHPAAPRMFLLGKVVEVAFYNMNRIRLEWSRIWNVIGEHFNAAGCNSNEAVAYFSVDALRQLSIKFLEKGELPNFRFQKDFLRPFEVIMVRNGSAQTRDLVVRCCAHLVEAHSSRLKSGWQNLFSVWTIAAGDPSTEIGEASFLTAQKVIEKRFKEDFPAFLDSFQEALKCLQEFACNQNQPDMNMEAIRLIRLCADYVSENSDKIDEAARRDDHLHKGLTADQHVWLRGWFPIFFELSCIINRCKLDVRTRSLTVMFEIMKHHGSDFRPEWWKDLLEIVFRIFDPSKMDDHRSDKREWMSTTCNHAMLSVVEVFTQFYTQLSVYALPMIYRQFGIFIRQQNEQLARCTISCLESLISQNGERFTEPMWEQTIELIRELFETTLPKSLLTWEPPNSNGIGSEDRTNGSDTLSSEQIVFCVVQNELVEAVSRIVLGDHRESKRDLQIDGLFTQMSPQLLLSICDALAESHTLAKQFNNNNGQRVLIWKARLLGSTKPNLINQETRSLSAMLAILFRLLYDLRAQSISDKIAIRVLEVVSLALSGYGEAESDTRRTAYGPVICELLRECIALPTNLIPVLGPEFPLKLCDLVETAEDQTMRKLLAELFRRIINFQTKSPTTVQEALEESQKSHISENFT

Target ORF information:

RefSeq Version	NM_001026627.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (agef-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026627.6

ORF Insert Sequence:

ATGAGCGAAT TGTCAAACGG TCCGAAGAAA TCGATTGGCA ATATGTTTCT GAGAAGTGGA 
ATCGAAAAGA TACTCGCTGA TCGGGATATC AAGCGAAAAG AGAACTTGCA GCTCAAAAAA 
GCGTGTGAAA GTGCTCTTGA AGAACTAAAA GCCGCGGAAG AGCAAGATGC CTCACCAAGC 
TCAAACGGAG AACACCTACC AGATGCGGGT GGCACCGCCG TCGAAGCCGA TCGATATTTC 
CTGCCATTCG AGCTCGCGTG CAACTCGAAA AGCCCGAAAA TCGTGATAAC CGCGTTGGAT 
TGCCTACAGA AATTGATCGC CTACGGACAT TTGACGGGTC GTGGCGCTGA TATTTCAAAT 
CCGGAACGAA AGTTAATTGA TCGAATTGTC GAAGCGATTT GTGCACCGTT TTTAGGTCAA 
GGAACTGATG AAACTGTACT TTTGCAGTTG ATTAAGGCTG TGCTTGCCGT TGTTCTCTCA 
ACTCACTGTG AAGTACACGG AGCATCACTT ATTCTCGCTG TTCGGACATG CTTCAACATT 
TATTTAACGT CAAAAAGCCC GATTAATCAG GCGACGGCTA AGGGAACACT TACACAGGTT 
ATCAATACGG TTTTTGGAAA TATGGAAAAG TTTGGAAATA TTAAAGACGA TGAGACGATT 
GTGAGAGAAG TTGTGGAGGT TCTTGTATCG AATACAATAT CCAATGAAGT GAGCGACGAA 
ACAAGTGAAG CCGGAGGAAC TCATCGACAG AATGGCTCAA CAATGGGTGA AAGTGAGGCT 
CCACTGGATG ATCAATTTAC ATTTATGAAT GCATATCAAA AGGATGCTTT TCTAGTATTC 
CGTGCTCTTT GTATTCTTGC ACAGAAGGAA GAAGGTGGTG CATCAAATGA AATGAGCCTT 
CGATCGAAAC TTCTTGCACT GGAAATGCTT CTTCTTGTAC TCCAAAACTC TTCATCAATC 
CTTCAATCCT CCCAGCCGTG TATAATAGTT ATTAAACGAA CGTTGTGTAT GGCATTAACC 
CGTAACGCGG TTAGCAATAA TATTCAAGTA TTTGAAAAGT CGCTGGCCAT ATTTGTGGAG 
CTATTGGACA AATTTAAAAC ACATCTCAAA GCTTCGATTG AGGTTTTCTT TAATAGTGTC 
ATTCTTCCGA TGCTTGATTC GAATACGTGT GCTTTTGAAC AGAAATGGAT TGTTCTTAAT 
ACAATTGGAA AAATCCTTGC GAATCCCCAA TCAGTTGTGG ATATGTTTGT GAATTACGAT 
TGTGATATGA CGTCACCGAA TCTATTCAAA TCAATTGTGG AAGTTGTGTC AAAGACAACA 
CGGACCACTA TCAATGAAAA TGCACCGCCC GCGCAGAAGG AAAAAGAACG AGCAATGCGG 
CTTCTGGGAT TAAGCTGCCT GACGGATCTT CTTCAATGTC TTGTTGATTG GTGGCAAGTG 
TGTGAAGTAC AGAAAATTAC AAGTGATATC GATGACGCGG AGCCCAGTGA GCAACAACAC 
GGTGAAACAT TCGAAGCATT TGAAACTCTA AAACAACAAA AAAATCTAAT GGAACAAGGC 
ATTCAAATAT TCTCGGAAAA ACCGAAAAAA GGACTGAAAT TCCTTCAAGA ACACGGTTTT 
GTTGGAACTG ATGCTGTAGA AGTTGCCGAG TTTATGATGA AAGAAGAACG GCTGAATAAG 
ACACAAGTTG GAGATTTTCT AGGCGATTCT GATGAATTTA ATAATTCTGT AATGCATGCA 
TATATTGATT TTTTGGATTT CTCGTCGATT GATATTCTCG CAGCGTTGAG AATGTTTTTG 
GAGAAATTTA GATTACCAGG AGAAGCTCAA AAAATCGATC GCCTAATGTT GAAATTCGCT 
TCGCGATACC TGGACTGTAA TCCACGCCAA GGAATATTTG CCTCAGCCGA CGCTGCCTAC 
GTACTCGCGT TCTCAATTAT CATGTTAACA ACGGATCTAC ATAATAAAAC AGTAAAGAAT 
AAAATGACGA AACAAGGATA TATTAATATG AATCGAGGTA TTAATGAAGG TGGAAATATT 
CCTGTTGAAC TGCTCGAAGC GATTTTCGAA GATATTTCGA AGAATGAGAT TAAGATGAGA 
GCCGGAGCCA CTGCTTTGCT CAGATCTCGG GTTACACCAG GACAAGGAGC ACTGGCAACT 
GATAAAGAAC GAAGAGCTAT GGCTGCACTG GAAATGGAGG CTTTGTCTGA AACTGCGAGA 
GCCCTAATGG AGTCTGCAAG TGACGCTGAT GCCTATTTCA CTCCAGCACA ACACCAACAT 
CACGTAAAAC CAATGTTCAA AATCTGCTGG ACACCATGCC TTGCCGCATT CTCAGTCGGT 
GTACAAATGT CGGATGATGA AGAAGAATGG TCTCTTTGTT TGAGAGGTTT TCGTCTGGGA 
GTACGTGCCG CGTGTGTTCT ACAAGCAACA CTTGAACGGA ATGCATTTAT ACAGGCACTT 
GCGAGATTTA CTCTTTTAAC GGCAAAAAAT TCATTGGGAG AAATGCGAGT GAAGAATATT 
GAAGCAATCA AGCTTTTACT ATTAATTGGT GATGAAGATG GAGAATATCT TGAAGAGAAT 
TGGGTTGATG TTATGAAATG TATGAGTTCT CTTGAATTAG TACAATTAAT TGGAACTGGA 
CTTAATTCTG CAATGTCTCA TGATACGGAT AGTAGTCGAC AGTACGTGAT GAAAGCCACC 
GGAGGAATCG ATGAAAAAAC GCTTCACTCT TTGCAAGATG CTCTCGGTGA AACATCATCT 
CAATCAGTTG TAGTGGCAAT TGATCGAATA TTCAATGGTT CCGCCCGTCT TTCAGCAGAA 
GCTATTGTAT ATTTTGTACG TGCTCTTTGT GCAGTCTCTC GAGAAGAGCT ATCACATCCG 
GCGGCTCCAC GAATGTTTTT ATTGGGAAAA GTTGTTGAAG TGGCGTTCTA TAATATGAAT 
AGAATTCGTT TGGAATGGAG TCGAATTTGG AATGTTATCG GTGAACATTT TAATGCGGCT 
GGATGTAATT CAAATGAAGC TGTTGCCTAT TTTTCGGTGG ATGCGCTGAG GCAGTTGTCG 
ATCAAATTTT TGGAAAAGGG AGAACTTCCG AATTTCCGAT TTCAGAAGGA TTTCTTACGG 
CCGTTTGAGG TAATAATGGT TCGAAACGGA AGTGCCCAGA CACGAGATCT TGTCGTTCGA 
TGCTGTGCTC ATTTAGTAGA AGCTCACTCG TCTCGACTCA AATCCGGATG GCAGAATCTC 
TTTTCAGTAT GGACAATTGC CGCCGGAGAC CCTTCTACTG AAATTGGTGA AGCATCATTC 
CTCACCGCTC AGAAAGTCAT CGAAAAACGT TTTAAAGAGG ATTTTCCGGC GTTTTTAGAC 
TCATTTCAAG AGGCGTTGAA GTGTTTACAA GAGTTTGCAT GTAATCAAAA TCAACCTGAT 
ATGAATATGG AAGCAATTCG ATTGATTCGA CTTTGTGCTG ACTACGTATC GGAGAATAGT 
GACAAAATCG ATGAGGCCGC CCGTCGTGAT GATCATCTTC ATAAGGGATT AACTGCTGAT 
CAACACGTAT GGCTACGTGG ATGGTTTCCA ATATTCTTTG AATTGAGTTG TATTATTAAT 
CGATGCAAAT TGGATGTTCG AACACGGAGC TTAACTGTCA TGTTTGAAAT TATGAAACAT 
CATGGATCGG ATTTTAGGCC AGAATGGTGG AAGGATCTGT TGGAAATCGT TTTTCGCATT 
TTTGATCCAT CGAAAATGGA CGATCATCGA AGTGATAAGC GAGAATGGAT GTCAACGACG 
TGTAATCATG CAATGTTGAG TGTTGTCGAA GTGTTCACAC AATTCTACAC TCAACTCTCC 
GTCTACGCAC TTCCAATGAT CTATCGTCAA TTTGGAATCT TTATTCGACA ACAAAACGAA 
CAGCTCGCCA GATGTACGAT TAGTTGCCTG GAATCACTAA TCTCACAGAA CGGAGAACGA 
TTCACTGAGC CAATGTGGGA ACAGACTATT GAATTGATTC GGGAGCTATT TGAGACGACA 
CTGCCGAAAT CGTTGCTCAC ATGGGAGCCG CCGAATTCTA ATGGAATTGG GTCGGAGGAT 
CGGACGAATG GATCCGACAC TCTCTCATCG GAACAAATCG TCTTCTGTGT TGTACAAAAT 
GAGCTCGTCG AAGCGGTCTC CCGGATTGTG CTCGGAGACC ATCGGGAAAG TAAACGAGAT 
CTTCAAATCG ATGGCCTATT CACTCAAATG TCACCACAAT TACTTTTGAG CATTTGTGAT 
GCTCTCGCGG AAAGTCATAC ACTGGCGAAA CAATTCAATA ATAATAATGG GCAACGTGTA 
TTGATTTGGA AAGCACGACT ACTTGGATCT ACGAAGCCCA ATTTGATCAA CCAAGAAACG 
CGATCGCTAA GTGCAATGTT GGCTATTCTA TTCCGCCTGC TCTACGATCT CCGAGCCCAA 
TCGATTTCTG ACAAAATTGC GATTCGAGTG TTAGAAGTCG TTTCACTTGC GCTCAGCGGC 
TACGGAGAAG CCGAATCCGA CACCAGGCGG ACTGCGTATG GACCAGTAAT TTGTGAATTG 
TTGAGAGAAT GCATTGCACT TCCGACCAAC TTGATTCCGG TACTTGGGCC GGAGTTTCCA 
TTAAAACTCT GCGATCTCGT TGAAACGGCC GAAGATCAAA CGATGCGAAA ACTGCTCGCC 
GAGCTCTTCC GACGAATAAT CAATTTTCAA ACGAAATCAC CAACAACTGT TCAAGAAGCG 
CTAGAAGAAT CTCAGAAATC CCATATTTCG GAAAATTTCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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agef-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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