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タンパク質単離・精製

Technical Resources

Genscriptタンパク質精製用レジン

精製をより簡単に！

タンパク質精製用レジン

目的のタンパク質の機能、構造、相互作用などの評価の際にタンパク質を精製することは不可欠です。 さまざまなタンパク質精製方法がありますが、その中で、アフィニティークロマトグラフィーは、標的タンパク質に対して高い選択性、高分解能、および高い結合能を持っており、非常に優れた精製方法となっています。 弊社では、バッチ/重力精製用のさまざまな使いやすいアフィニティー精製用レジンを提供しており、これらを用いて、未精製のサンプルから目的のタンパク質や抗体を効率的に簡便に、かつ信頼性の高い方法で分離できます。

特長

アプリケーション


アプリケーション 製品 使用リガンド 結合能 容量 製品番号.
抗体精製 Protein A Resin Recombinant protein A >20 mg human IgG/ml settled resin 5 ml L00210
Protein A Resin FF Recombinant protein A on FPLC-compatible resin > 40 mg human IgG/ml settled resin 5 ml L00464
Protein G Resin Recombinant protein G >20 mg sheep IgG/ml settled resin 5 ml L00209
Protein G Resin FF Recombinant protein G on FPLC-compatible resin ≥ 20 mg human IgG/m settled resin 5/25/100 ml L00664
Protein L Resin Recombinant protein L >15 mg rabbit IgG/ml settled resin 2.5 ml L00239
アルカリ耐性抗体精製用レジン Monofinity A Resin Recombinant alkaline tolerant protein A >30 mg human IgG/ml settled resin 25/200 ml
1/5/10 L
custom		 L00433
抗体精製 (プレパックカラム) Monofinity A Resin Prepacked Column Recombinant alkaline tolerant protein A in a prepacked column >30 mg human IgG/ml settled resin 1/5/25 ml L00433
Protein A Resin FF Prepacked Column Recombinant alkaline tolerant protein A on FPLC-compatible resin in a prepacked column > 40 mg human IgG/ ml settled resin 2 /5 /25 ml L00680
Protein G Resin FF Prepacked Column Recombinant protein G on FPLC-compatible resin in a prepacked column ≥ 20 mg human IgG/ml settled resin 2 /5 /25 ml L00681
Hisタグ付きタンパク質の精製 High Affinity Ni-charged Resin Ni-NTA-charged resin 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin 10 ml
25 ml
500 ml		 L00223
Ni Resin FF Ni-IDA-charged FPLC-compatible resin >40 mg 6xhistidine-tagged protein /ml settled resin 5 ml
25 ml		 L00465
High Affinity Ni-Charged Resin FF Ni-NTA-charged FPLC-compatible resin >45 mg of histidine-tagged protein /ml settled resin 5/25/100 ml L00666
Hisタグ付きタンパク質の精製 (プレパックカラム) High Affinity Ni-Charged Resin FF Prepacked Column Ni-NTA-charged FPLC-compatible resin in a prepacked column >45 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00683
Ni-IDA Resin FF Prepacked Column Ni-IDA-charged FPLC-compatible resin in a prepacked column >40 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00684
GSTタグ付きタンパク質の精製 Glutathione Resin Glutathione 35-45 mg GST-tagged protein (26kD) /ml settled resin  10 ml L00206
GST Fusion Protein Purification Kit 1 kit L00207
DYKDDDDK(Flag)タグ付きタンパク質の精製 MonoRab™ Anti-DYKDDDDK Affinity Resin MonoRab™ Anti-DYKDDDDK antibody Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
25 ml		 L00766
Anti-DYKDDDDK G1 Affinity Resin Anti-DYKDDDDK G1 antibody Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
10 ml
25 ml		 L00432
ビオチン化タンパク質、抗体、レクチンの精製 Streptavidin Resin Streptavidin 120 nmol D-Binotin/ml settled resin 5 ml L00353
チオール基を含むペプチドやタンパク質の固相化などに High-Affinity Iodoacetyl Resin 　 >9 mg Goat IgG/ml settled resin 10 ml L00403
1 kit L00404
High-Affinity Antibody Purification Kit
免疫沈降用レジン Anti-His Affinity Resin THE™ anti-His monoclonal antibody >0.8 mg of histidine-tagged protein /ml settled resin 1/5 ml L00439
Chicken IgY Precipitating Resin Goat Anti-Chicken IgY Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes. 1 ml L00405
Anti-DYKDDDDK IP Resin Mouse anti-DYKDDDDK monoclonal antibody Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. 1 ml L00425
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  • Gow DJ., et al. Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor. Mol Ther. 2014 Sep;22(9):1580-92.
  • Zilio N., et al. A novel histone deacetylase complex in the control of transcription and genome stability. Mol Cell Biol. 2014 Sep;15;34(18):3500-14.
  • Zhang H., et al. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 2014 Sep;53:67-77.
  • Ma L., et al. Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system. Mol Cell Proteomics. 2014 Jul;13(7):1659-75.
  • Liu W., et al. In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology. 2014 May;155(5):1738-50.
  • Asavapanumas N., et al. Unique neuromyelitis optica pathology produced in na?ve rats by intracerebral administration of NMO-IgG. Acta Neuropathol. 2014 Apr;127(4):539-51.
  • Lin H., et al. A novel human Fab antibody for Trop2 inhibits breast cancer growth in vitro and in vivo. Int J Cancer. 2014 Mar 1;134(5):1239-49.
  • Zhang D., et al. The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress. PLoS Genet. 2014 Jan;10(1):e1004061.
  • Lan JF., et al. Prohibitin Interacts with envelope proteins of white spot syndrome virus and prevents infection in the red swamp crayfish, Procambarus clarkii. J Virol. 2013 Dec;87(23):12756-65.
  • Chen Y., et al. Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase. J Virol. 2013 Jun;87(11):6296-305.
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  • de Oliveira S, et al. Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J Immunol. 2013 Apr 15;190(8):4349-59.
  • Tian M., et al. The combined use of photoaffinity labeling and surface plasmon resonance-based technology identifies multiple salicylic acid-binding proteins. Plant J. 2012 Dec;72(6):1027-38.
  • Lei Y., et al. The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy. Immunity. 2012 Jun 29;36(6):933-46.
  • Worzfeld T., et al. ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis. J Clin Invest. 2012 Apr 2;122(4):1296-305.
  • Rouet R., et al. Expression of high-affinity human antibody fragments in bacteria. Nat Protoc. 2012 Feb 2;7(2):364-73.
  • Hu J., et al. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding. Plant Physiol. 2011 Jun;156(2):856-72.
  • Staron M., et al. Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex. Blood. 2011 Jun 30;117(26):7136-44.
  • Newell PD., et al. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS Biol. 2011 Feb 1;9(2):e1000587.