ペプチド修飾

概要

弊社では、研究者の様々な要望に応えるため多種類のペプチド修飾を行っています。ペプチドを修飾することにより、安定性を向上させ、生物学的機能解明のために構造を変更し、さらに抗体の開発や製造のために免疫原性を増強させることが可能です。ペプチドの末端や内部に対する様々な修飾に加え、イメージングや検出のニーズに対応するためのペプチド標識やペプチド‐タンパク質の複合体作製も行っています。

以下は弊社が提供するペプチド修飾の種類

• N末端修飾

  BSA conjugation on N terminal -NH2	KLH conjugation on N terminal -NH2	OVA conjugation on N terminal -NH2	Acetylation
  Biotin	Biotin-Ahx	5-FAM	5-FAM-Ahx
  6-FAM	6-FAM-Ahx	2-Abz	4-Abz
  Cy3	Cy5	Cy5.5	Cy7
  DABCYL	Dansyl	Dansyl-Ahx	FITC-Ahx
  FITC-PEG2	5-TMR	6-TMR	Rhodamine B
  MCA	3-Maleimide	6-Maleimide	SMCC
  Acryl	Alloc	Benzoyl	CBZ
  Fmoc	Br-Ac	Cl-Ac	Aminooxy
  DOTA	NOTA	1-Nap	2-Nap
  Succinylation	Glutaric acid	Butyric acid,C4	Hexanoic acid,C6
  Octanoic acid,C8	Nonanoic acid,C9	Decanoic acid,C10	Lauric acid,C12
  Myristic acid,C14	Palmitic acid,C16	Stearic acid,C18	Arachidic acid,C20
  L-Lactic acid	D-Lactic acid	(R)-Lipoic acid	2-Mercaptoacetic acid
  2-Azidoacetic acid	4-Azidobutyric acid	6-Azidohexanoic acid	Propiolic acid
  5-Hexynoicacid	Trans-Cinnamic acid	Trans-Crotonic acid	DBCO
  Methyltetrazine	TCO

• C末端修飾

  Amidation	Alcohol
  AMC	Bzl
  Cysteamide	EDA-Biotin
  Ester (OEt)	Ester (OMe)
  Glu(EDANS)	Hydrazine
  MPAA	NHEt
  Nhisopen	NHMe
  TBzl	p-Nitroanilide
  Tyr (3-NO2)	BSA conjugation on C terminal -COOH
  KLH conjugation on C terminal -COOH	OVA conjugation on C terminal -COOH

• 非天然アミノ酸等、特殊アミノ酸
• 安定同位体標識ペプチド

  {Arg(13C6,15N4)}

  {Ile(13C6,15N)}

  {Leu(13C6,15N)}

  {Lys(13C6,15N2)}

  {Phe(13C9,15N)}

  {Pro(13C5,15N)}

  {Val(13C5,15N)}

• 蛍光色素標識ペプチド/FRETペア

  2-Abz (N-Terminal)	FITC-Ahx (N-Terminal)
  4-Abz (N-Terminal)	FITC-PEG2 (N-Terminal)
  5-FAM (N-Terminal)	MCA (N-Terminal)
  5-FAM-Ahx (N-Terminal)	Rhodamine B (N-Terminal)
  6-FAM (N-Terminal)	AMC (C-Terminal)
  6-FAM-Ahx (N-Terminal)	{ACC} (7-Aminocoumarin-4-Acetic acid)
  5-TMR (N-Terminal)	MCA/Lys(DNP)
  6-TMR (N-Terminal)	2-Abz/Lys(DNP)
  Cy3 (N-Terminal)	4-Abz/Lys(DNP)
  Cy5 (N-Terminal)	2-Abz/Tyr(3-NO2)
  Cy5.5 (N-Terminal)	4-Abz/Tyr(3-NO2)
  Cy7 (N-Terminal)	DABCYL/Glu(EDANS)
  Dansyl (N-Terminal)	DABCYL/EDANS
  Dansyl-Ahx (N-Terminal)

• ペプチドとの複合体作製

  BSA (-COOH of C terminal)

  BSA Conjugation on cysteine

  KLH (-NH2 of N terminal)

  KLH Conjugation on cysteine

  OVA (-COOH of C terminal)

  OVA (-NH2 of N terminal)

  OVA Conjugation on cysteine

• その他（MAPS, PEGylation, 環状化）

  MAPS	PEGylation	Cyclic modifications	Disulfide Bridges	Other
  MAPS Asymmetric 2 branches (C-Terminal)	{PEG1} NH2-(PEG)1-CH2COOH	Head to tail amide cyclic	Random Disulfide Bridge	Dimer ( Inter-Disulfide bridge)
  MAPS Asymmetric 4 branches (C-Terminal)	{PEG1-propionic acid} NH2-PEG1-CH2CH2COOH	Amide cyclic (Side chain)	Mono Disulfide bridge
  MAPS Asymmetric 8 branches (C-Terminal)	{PEG2} NH2-(PEG)2-CH2COOH	Stapled peptide(S5/S5)	Double Disulfide bridge
  	{PEG3} NH2-(PEG)3-CH2CH2COOH	Stapled peptide(R8/S5)	Triple Disulfide Bridge
  	{PEG4} NH2-(PEG)4-CH2CH2COOH	Mono Thioether Bridge
  	{PEG5} NH2-(PEG)5-CH2CH2COOH	Thioester (C-terminal)
  	{PEG6} NH2-(PEG)6-CH2CH2COOH	Amide cyclic (Head to side chain)
  	{PEG8} NH2-(PEG)8-CH2CH2COOH	Amide cyclic (Side chain to tail)
  	{PEG11} NH2-(PEG)11-CH2COOH
  	{PEG12} NH2-(PEG)12-CH2CH2COOH

* For your convenience, modifications can easily be added when you request a quote online. If you don't find the modification you are looking for, feel free to your technical account manager for more information.