Sec71 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec71
Entrez Gene ID	34785
Full Name	Secretory 71
Synonyms	BG:DS00797.7,BIG1,CG7578,Dmel\CG7578,sec71
General protein information	Preferred Names Secretory 71 Names CG7578 gene product from transcript CG7578-RA CG7578-PA Sec71 ortholog Sec71-PA sec71 secretory 71
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34D1-34D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135831.3	NP_609675.2	secretory 71

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification of Sec71 as a guanine nucleotide exchange factor for Arf1 that preferentially interacts with its GDP-bound form. Like Arf1, Sec71 is also important for dendrite pruning, but not for apoptosis of sensory neurons.
Title: Sec71 functions as a GEF for the small GTPase Arf1 to govern dendrite pruning of

The following Sec71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16725	NM_135831.3 Latest version!	Drosophila melanogaster secretory 71 (Sec71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec71 ( NM_135831.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609675.2 Drosophila melanogaster secretory 71 (Sec71), mRNA.

Price & Availability↑

CloneID	OFa16725
Clone ID Related Accession (Same CDS sequence)	NM_135831.3
Accession Version	NM_135831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 71
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135831.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACAACA ACTCCACAAA AACCAAGGAA ATGTTCATCG TGCGTGCTCT AGAAAAGATC 
CTTGCCGATA AGGACATACG GCGCTCCCAT CACTCGCAGC TGAAGAAGTC CTGCGATTCG 
GCGCTGGAGC AGATTAAGGC GGAGCTAATC AGTGCCGGCC AGATCGCAGA GGGCAATGAG 
CTGCCCTGTG CCGCACTCCC GCTGCCCAAG AATGATGCAG CGAGCATCAT AAATGCGGAG 
ACCTACTTTC TCCCCTTCGA GCTTGCCTGC AAGAGCCGCT CGCCCAGGAT CGTGGTCACC 
GCACTGGACT GCCTGCAGAA ACTCATTGCC TATGGCCATT TGACAGGATC CATTCAGGAC 
TCGGCCAATC CGGGTCACCT GCTCATCGAC CGTATCGTTG TGACCATATA TGGCTGCTTC 
AGTGGTCCCC AGACGGACGA GGCCGTCCAA CTGCAGATAA TAAAGGCTCT GCTCACGGTG 
GTCACCTCGC AGCATGTGGA AATCCATGAA TTCACACTGC TGCAAGCTGT GCGCACCTGC 
TACGACATCT ATTTGTCCAG CAAGAACCTG GTCAATCAGA CCACAGCACG CGCTACGCTC 
ACCCAAATGT TGAACGTGAT ATTTGCCCGC ATGGAGAATC AAGTGTACGA GCTACCACCT 
CCCAATTCCA ATCCCACCAA CGGCAGCATC CACTCGGAGG ATTGCAATGG CTCGGGAGAG 
GAGTCGCTGC GGGATTCCGA CGAAGTAATT GCCTCGGAAC TGCTGGCGGA GATCATATCA 
GCTGCCTACA ATGAGGCGAT GAAGGATCAG GAATCGGTCG GTGAGCCAGA GCCAACACTT 
AATGGAAACG ACTACTCCTC GCACTCGGAT CACGACAGTG TGGAGCTGCA CAGCGAAAAC 
GATGCGGTTG TAACGGCTAA GTTTACGCAC ATCCTGCAGA AAGATGCTTT TCTCGTGTTC 
CGGGCACTGT GCAAGCTATC GATGAAGCCT TTGCCGGATG GACATCCAGA TCCGAAATCG 
CACGAGCTGC GTTCCAAGGT GCTGTCATTG CATCTGCTGC TGCTCATCCT CCAGAATGCC 
GGGCCCGTCT TCCGCTCCAA CGAGATGTTC ATCATGGCCA TTAAGCAGTA CCTGTGCGTG 
GCCTTGTCAA ACAACGGAGT CAGTCTGGTG CCGGAGGTCT TCGAGCTGTC GCTTTCAATC 
TTCGTTGCCC TACTCTCGAA CTTCAAGGTG CATCTTAAGC GGCAGATAGA GGTGTTCTTC 
AAGGAAATCT TCCTAAACAT TCTTGAGGCG AACTCAAGCA GCTTCGAGCA CAAATGGATG 
GTAATCCAAG CGCTGACACG TATTTGTGCT GACGCCCAGT CCGTGGTGGA TATCTATGTT 
AATTACGATT GCGACTTTTC GGCTGCAAAC CTTTTTGAGA GACTGGTCAA CGATCTTTCG 
AAAATTGCCC AGGGTCGTCA GGCTCTCGAA CTGGGCGCCA ATCCGATGCA AGAGAAATCG 
ATGCGCATTC GCGGCCTGGA GTGTCTTGTC TCCATTCTTA AGTGCATGGT AGAGTGGAGT 
AAGGACTTGT ATGTTAATCC AAACATGCCG GTTCCACCTA TGCAAGTCCA ATCGCCGACA 
AGCACTGAGC AGGATCAGGC GGACACAACT ATCCAAACGA TGCACAGTGG TTCCAGTCAT 
AGTTTGAACT CCAATCAGGA GCAACTACAG GATCTTCCCG AGGCATTGGA GGAGCGCAAG 
ATGCGCAAGG AAGTGATGGA AACAGGCATT GAGTTATTCA ATCGTAAGCC TCAGAAAGGA 
GTGCAATTCC TGCAGGAGAA GCAGTTGCTG GGTGCCACAT GCGGGGACAT TGCGCGCTGG 
CTGCACGAGG ACGAACGACT GGACAAGACA GTGATCGGAA ACTACATTGG CGAGAATGAC 
GACCACTCCA AGGAAGTGAT GTGCGCTTAC ATCGATGCCT TTGACTTTCG CCAAATGGAG 
GTGGTGGCCG CCTTGAGATT TCTTCTCGAG GGGTTCCGCC TGCCAGGAGA AGCACAAAAA 
ATCGATCGGC TGATGGAGAA GTTCGCCAGT AGATACTGCG AATGCAATCC GAAGAACCAG 
CTATTCCAAA GCGCAGACAC CGTCTACGTG CTGGCATTCA GCATCATTAT GCTGACCACG 
GATCTTCATT CGCCGCAGGT CAAGCACAAG ATGACCAAGG AGCAGTACAT TAAAATGAAC 
CGCGGCATCA GCGACAGCAA GTCCGATTTG CCCGAGGAGT ACTTGTCGTC CATCTACGAC 
GAGATTTCTG AACACGAAAT TAAGATGAAG AACAACTCCG GTATGCTTCA ACAGGCGAAA 
CCCACTGGAA AGCAGGCCTT CATAACGGAG AAACGCAGAA AGCTGTTGTG GAACATGGAG 
ATGGAGGTCA TCTCGCTGAC GGCCACCAAT CTAATGCAGT CAGTTTCGCA CGTCAAGTCA 
CCCTTCACCT CAGCGAAACA CTTGGAGCAT GTCCGGCCCA TGTTCAAAAT GGCTTGGACA 
CCATTTCTGG CCGCTTTCTC TGTGGGTCTC CAGGACTGCG ACGATCCTGA GATTGCTACA 
CTCTGCTTGG ATGGTATACG TTGTGCTATT CGAATCGCAT GCATCTTCCA CATGTCCCTG 
GAGCGAGATG CCTATGTACA AGCCCTGGCC AGGTTTACTC TCCTGAATGC TAACTCGCCC 
ATCAACGAAA TGAAGGCCAA GAATATCGAT ACCATCAAGA CGCTTATAAT GGTAGCCCAC 
ACGGATGGCA ATTATCTGGG CAGCAGCTGG CTGGATATAG TGAAGTGCAT TAGCCAGTTG 
GAGCTGGCCC AACTGATCGG CACTGGGGTG CGGCCCCAGT TTCTTTCTGG AGCGCAGACA 
ACGCTCAAGG ACTCGCTTAA TCCCAGCGTG AAAGAACACA TCGGCGAGAC GAGCAGCCAG 
AGCGTGGTGG TCGCAGTCGA TCGTATTTTC ACCGGCTCAA TGCGACTGGA TGGCGATGCT 
ATCGTGGACT TCGTGAAGGC CCTGTGCCAG GTGTCTGTGG ATGAGCTTCA GCAGCAGCAA 
CCGAGGATGT TCTCCTTGCA AAAGATAGTG GAAATTAGTT ACTACAACAT GGAGCGTATT 
CGTCTGCAGT GGTCACGCAT TTGGCAAGTT TTGGGTGAGC ACTTTAACGC GGTCGGATGC 
AATAGCAACG AGGAGATCTC ATTTTTCGCC CTGGACTCAC TGCGTCAGTT GTCGATGAAG 
TTCATGGAGA AGGGCGAGTT CAGTAATTTC CGCTTCCAGA AGGATTTCCT GCGTCCCTTT 
GAGCATATCA TGAAGAAAAA CGCATCGCCG GCAATACGAG ATATGGTGGT GCGCTGCATT 
GCCCAGATGG TAAACTCACA GGCGCATAAC ATCCGTTCCG GCTGGAAGAA TATCTTTAGC 
ATTTTCCACC TGGCAGCGGG AGACAACGAA GAGCCAATTG TGGAGCTGGC CTTCCAAACC 
ACGGGCAAAA TCATCGGTGA TCTGTACAAG CGTCAGTTCG CCATTATGGT GGACTCGTTC 
CAGGATGCGG TCAAGTGCCT GTCAGAGTTC GCCACCGCCA GATTCCCCGA TACCAGCATG 
GAAGCCATAC GTCTGGTCCG TACCTGCGCG CAGTGCGTCC ACGAGGCACC ACAACTGTTT 
GCGGAGCATG CCGGCATGGA GAACGACGCC TCGGTGGCCG AGGAGGATCG AGTCTGGGTG 
CGCGGCTGGT TTCCGATGCT ATTCTCGCTT TCCTGCGTGG TCAATCGCTG CAAATTGGAT 
GTGCGTACTC GCGCCTTAAC CGTGCTTTTT GAGATTGTGA AGACGTATGG TGAGAGCTTC 
AAGCCCCATT GGTGGAAGGA TCTCTTCAAT GTGATCTTCC GTATCTTCGA CAACATGAAA 
TTGCCGGAGC ACGTCACCGA GAAGTCCGAA TGGATGACGA CCACATGCAA CCACGCCTTG 
TACGCTATTA TTGATGTCTT CACGCAGTAT TTCGATGTTC TTGGTCATCT GCTGCTGGAG 
GAGCTCTTCG CCCAGCTGCA TTGGTGTGTT CAGCAGAGTA ACGAGCAGTT GGCGCGATCT 
GGCACCAATT GCCTGGAGAA CCTCGTCATT TCGAATGGAT TCAAGTTCAA CGAGTCCACC 
TGGGACAAGA CGTGCCAGTG CATCCTGGAC ATCTTCAACG CCACTTTGCC GCAGGATCTC 
CTCAGTTGGC GGCCGAAAGC ACATTCCAGT AACAATATAC CCCAGGAGCA CAACCACTTT 
GAGGCGCTGC ATATCCGCTG CGTAGTCCAG CTGGAACTGA TACAGACCAT GGATAACATT 
GTCTTTTTCC CGGCCACGTC GCGCAAGGAG GATGCCGAAA CGCTGGCCCA GGCGGCGGCA 
GACTTAACAG GCGGCAGGAG CGGTTCGCAG TCGCAGCTGC TGGAGTGCCA GCGGGAGGAG 
CAGGGAATGT ACGGCTATCT GAGAACCCGC CAGCTGCTCA CCCTGGCCGA CTGTCTGATG 
CAGTCGCACC GTTTTGCCAA GCGCTTCAAC GCCGATCACG ACCAACGCAG CCTGCTTTGG 
CGGGCGGGAT TCAAGGGATC TGTTAAACCG AATCTGCTGA AGCAGGAGAC CTCGTCGCTG 
GCCTGCGTCC TGCGCATTTT CTTCAAGATG TACGGCGACG AGAATAGACG CAGCGATTGG 
CCCGGCATCG AGCAGGAACT GGTGCAGGTC TGCAAGGAGG CACTGGGCTA CTATTTGAGT 
TTGCAGAGCG AGGCACACCG AGATGCGTGG ACATCGCTGC TGCTGCTCAT CCTGACGCGC 
CTGCTCAAGA TGTCCGATGC CAGGTTCGCC ACCCACGTTT CCAACTACTA CAGCCTGCTG 
TGCGAGATGA TGTGCTTCGA CCTCAAGCCC GAACTGAGAA GTGTCCTTAG GCGTGTGTTC 
ATGCGCATCG GTCCAGTATT CAATATAATG AGCGTTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609675.2
CDS	328..5289
Translation	MHNNSTKTKEMFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLPKNDAASIINAETYFLPFELACKSRSPRIVVTALDCLQKLIAYGHLTGSIQDSANPGHLLIDRIVVTIYGCFSGPQTDEAVQLQIIKALLTVVTSQHVEIHEFTLLQAVRTCYDIYLSSKNLVNQTTARATLTQMLNVIFARMENQVYELPPPNSNPTNGSIHSEDCNGSGEESLRDSDEVIASELLAEIISAAYNEAMKDQESVGEPEPTLNGNDYSSHSDHDSVELHSENDAVVTAKFTHILQKDAFLVFRALCKLSMKPLPDGHPDPKSHELRSKVLSLHLLLLILQNAGPVFRSNEMFIMAIKQYLCVALSNNGVSLVPEVFELSLSIFVALLSNFKVHLKRQIEVFFKEIFLNILEANSSSFEHKWMVIQALTRICADAQSVVDIYVNYDCDFSAANLFERLVNDLSKIAQGRQALELGANPMQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNMPVPPMQVQSPTSTEQDQADTTIQTMHSGSSHSLNSNQEQLQDLPEALEERKMRKEVMETGIELFNRKPQKGVQFLQEKQLLGATCGDIARWLHEDERLDKTVIGNYIGENDDHSKEVMCAYIDAFDFRQMEVVAALRFLLEGFRLPGEAQKIDRLMEKFASRYCECNPKNQLFQSADTVYVLAFSIIMLTTDLHSPQVKHKMTKEQYIKMNRGISDSKSDLPEEYLSSIYDEISEHEIKMKNNSGMLQQAKPTGKQAFITEKRRKLLWNMEMEVISLTATNLMQSVSHVKSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIATLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCISQLELAQLIGTGVRPQFLSGAQTTLKDSLNPSVKEHIGETSSQSVVVAVDRIFTGSMRLDGDAIVDFVKALCQVSVDELQQQQPRMFSLQKIVEISYYNMERIRLQWSRIWQVLGEHFNAVGCNSNEEISFFALDSLRQLSMKFMEKGEFSNFRFQKDFLRPFEHIMKKNASPAIRDMVVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDNEEPIVELAFQTTGKIIGDLYKRQFAIMVDSFQDAVKCLSEFATARFPDTSMEAIRLVRTCAQCVHEAPQLFAEHAGMENDASVAEEDRVWVRGWFPMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPHWWKDLFNVIFRIFDNMKLPEHVTEKSEWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCVQQSNEQLARSGTNCLENLVISNGFKFNESTWDKTCQCILDIFNATLPQDLLSWRPKAHSSNNIPQEHNHFEALHIRCVVQLELIQTMDNIVFFPATSRKEDAETLAQAAADLTGGRSGSQSQLLECQREEQGMYGYLRTRQLLTLADCLMQSHRFAKRFNADHDQRSLLWRAGFKGSVKPNLLKQETSSLACVLRIFFKMYGDENRRSDWPGIEQELVQVCKEALGYYLSLQSEAHRDAWTSLLLLILTRLLKMSDARFATHVSNYYSLLCEMMCFDLKPELRSVLRRVFMRIGPVFNIMSVK

Target ORF information:

RefSeq Version	NM_135831.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 71 (Sec71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135831.3

ORF Insert Sequence:

ATGCACAACA ACTCCACAAA AACCAAGGAA ATGTTCATCG TGCGTGCTCT AGAAAAGATC 
CTTGCCGATA AGGACATACG GCGCTCCCAT CACTCGCAGC TGAAGAAGTC CTGCGATTCG 
GCGCTGGAGC AGATTAAGGC GGAGCTAATC AGTGCCGGCC AGATCGCAGA GGGCAATGAG 
CTGCCCTGTG CCGCACTCCC GCTGCCCAAG AATGATGCAG CGAGCATCAT AAATGCGGAG 
ACCTACTTTC TCCCCTTCGA GCTTGCCTGC AAGAGCCGCT CGCCCAGGAT CGTGGTCACC 
GCACTGGACT GCCTGCAGAA ACTCATTGCC TATGGCCATT TGACAGGATC CATTCAGGAC 
TCGGCCAATC CGGGTCACCT GCTCATCGAC CGTATCGTTG TGACCATATA TGGCTGCTTC 
AGTGGTCCCC AGACGGACGA GGCCGTCCAA CTGCAGATAA TAAAGGCTCT GCTCACGGTG 
GTCACCTCGC AGCATGTGGA AATCCATGAA TTCACACTGC TGCAAGCTGT GCGCACCTGC 
TACGACATCT ATTTGTCCAG CAAGAACCTG GTCAATCAGA CCACAGCACG CGCTACGCTC 
ACCCAAATGT TGAACGTGAT ATTTGCCCGC ATGGAGAATC AAGTGTACGA GCTACCACCT 
CCCAATTCCA ATCCCACCAA CGGCAGCATC CACTCGGAGG ATTGCAATGG CTCGGGAGAG 
GAGTCGCTGC GGGATTCCGA CGAAGTAATT GCCTCGGAAC TGCTGGCGGA GATCATATCA 
GCTGCCTACA ATGAGGCGAT GAAGGATCAG GAATCGGTCG GTGAGCCAGA GCCAACACTT 
AATGGAAACG ACTACTCCTC GCACTCGGAT CACGACAGTG TGGAGCTGCA CAGCGAAAAC 
GATGCGGTTG TAACGGCTAA GTTTACGCAC ATCCTGCAGA AAGATGCTTT TCTCGTGTTC 
CGGGCACTGT GCAAGCTATC GATGAAGCCT TTGCCGGATG GACATCCAGA TCCGAAATCG 
CACGAGCTGC GTTCCAAGGT GCTGTCATTG CATCTGCTGC TGCTCATCCT CCAGAATGCC 
GGGCCCGTCT TCCGCTCCAA CGAGATGTTC ATCATGGCCA TTAAGCAGTA CCTGTGCGTG 
GCCTTGTCAA ACAACGGAGT CAGTCTGGTG CCGGAGGTCT TCGAGCTGTC GCTTTCAATC 
TTCGTTGCCC TACTCTCGAA CTTCAAGGTG CATCTTAAGC GGCAGATAGA GGTGTTCTTC 
AAGGAAATCT TCCTAAACAT TCTTGAGGCG AACTCAAGCA GCTTCGAGCA CAAATGGATG 
GTAATCCAAG CGCTGACACG TATTTGTGCT GACGCCCAGT CCGTGGTGGA TATCTATGTT 
AATTACGATT GCGACTTTTC GGCTGCAAAC CTTTTTGAGA GACTGGTCAA CGATCTTTCG 
AAAATTGCCC AGGGTCGTCA GGCTCTCGAA CTGGGCGCCA ATCCGATGCA AGAGAAATCG 
ATGCGCATTC GCGGCCTGGA GTGTCTTGTC TCCATTCTTA AGTGCATGGT AGAGTGGAGT 
AAGGACTTGT ATGTTAATCC AAACATGCCG GTTCCACCTA TGCAAGTCCA ATCGCCGACA 
AGCACTGAGC AGGATCAGGC GGACACAACT ATCCAAACGA TGCACAGTGG TTCCAGTCAT 
AGTTTGAACT CCAATCAGGA GCAACTACAG GATCTTCCCG AGGCATTGGA GGAGCGCAAG 
ATGCGCAAGG AAGTGATGGA AACAGGCATT GAGTTATTCA ATCGTAAGCC TCAGAAAGGA 
GTGCAATTCC TGCAGGAGAA GCAGTTGCTG GGTGCCACAT GCGGGGACAT TGCGCGCTGG 
CTGCACGAGG ACGAACGACT GGACAAGACA GTGATCGGAA ACTACATTGG CGAGAATGAC 
GACCACTCCA AGGAAGTGAT GTGCGCTTAC ATCGATGCCT TTGACTTTCG CCAAATGGAG 
GTGGTGGCCG CCTTGAGATT TCTTCTCGAG GGGTTCCGCC TGCCAGGAGA AGCACAAAAA 
ATCGATCGGC TGATGGAGAA GTTCGCCAGT AGATACTGCG AATGCAATCC GAAGAACCAG 
CTATTCCAAA GCGCAGACAC CGTCTACGTG CTGGCATTCA GCATCATTAT GCTGACCACG 
GATCTTCATT CGCCGCAGGT CAAGCACAAG ATGACCAAGG AGCAGTACAT TAAAATGAAC 
CGCGGCATCA GCGACAGCAA GTCCGATTTG CCCGAGGAGT ACTTGTCGTC CATCTACGAC 
GAGATTTCTG AACACGAAAT TAAGATGAAG AACAACTCCG GTATGCTTCA ACAGGCGAAA 
CCCACTGGAA AGCAGGCCTT CATAACGGAG AAACGCAGAA AGCTGTTGTG GAACATGGAG 
ATGGAGGTCA TCTCGCTGAC GGCCACCAAT CTAATGCAGT CAGTTTCGCA CGTCAAGTCA 
CCCTTCACCT CAGCGAAACA CTTGGAGCAT GTCCGGCCCA TGTTCAAAAT GGCTTGGACA 
CCATTTCTGG CCGCTTTCTC TGTGGGTCTC CAGGACTGCG ACGATCCTGA GATTGCTACA 
CTCTGCTTGG ATGGTATACG TTGTGCTATT CGAATCGCAT GCATCTTCCA CATGTCCCTG 
GAGCGAGATG CCTATGTACA AGCCCTGGCC AGGTTTACTC TCCTGAATGC TAACTCGCCC 
ATCAACGAAA TGAAGGCCAA GAATATCGAT ACCATCAAGA CGCTTATAAT GGTAGCCCAC 
ACGGATGGCA ATTATCTGGG CAGCAGCTGG CTGGATATAG TGAAGTGCAT TAGCCAGTTG 
GAGCTGGCCC AACTGATCGG CACTGGGGTG CGGCCCCAGT TTCTTTCTGG AGCGCAGACA 
ACGCTCAAGG ACTCGCTTAA TCCCAGCGTG AAAGAACACA TCGGCGAGAC GAGCAGCCAG 
AGCGTGGTGG TCGCAGTCGA TCGTATTTTC ACCGGCTCAA TGCGACTGGA TGGCGATGCT 
ATCGTGGACT TCGTGAAGGC CCTGTGCCAG GTGTCTGTGG ATGAGCTTCA GCAGCAGCAA 
CCGAGGATGT TCTCCTTGCA AAAGATAGTG GAAATTAGTT ACTACAACAT GGAGCGTATT 
CGTCTGCAGT GGTCACGCAT TTGGCAAGTT TTGGGTGAGC ACTTTAACGC GGTCGGATGC 
AATAGCAACG AGGAGATCTC ATTTTTCGCC CTGGACTCAC TGCGTCAGTT GTCGATGAAG 
TTCATGGAGA AGGGCGAGTT CAGTAATTTC CGCTTCCAGA AGGATTTCCT GCGTCCCTTT 
GAGCATATCA TGAAGAAAAA CGCATCGCCG GCAATACGAG ATATGGTGGT GCGCTGCATT 
GCCCAGATGG TAAACTCACA GGCGCATAAC ATCCGTTCCG GCTGGAAGAA TATCTTTAGC 
ATTTTCCACC TGGCAGCGGG AGACAACGAA GAGCCAATTG TGGAGCTGGC CTTCCAAACC 
ACGGGCAAAA TCATCGGTGA TCTGTACAAG CGTCAGTTCG CCATTATGGT GGACTCGTTC 
CAGGATGCGG TCAAGTGCCT GTCAGAGTTC GCCACCGCCA GATTCCCCGA TACCAGCATG 
GAAGCCATAC GTCTGGTCCG TACCTGCGCG CAGTGCGTCC ACGAGGCACC ACAACTGTTT 
GCGGAGCATG CCGGCATGGA GAACGACGCC TCGGTGGCCG AGGAGGATCG AGTCTGGGTG 
CGCGGCTGGT TTCCGATGCT ATTCTCGCTT TCCTGCGTGG TCAATCGCTG CAAATTGGAT 
GTGCGTACTC GCGCCTTAAC CGTGCTTTTT GAGATTGTGA AGACGTATGG TGAGAGCTTC 
AAGCCCCATT GGTGGAAGGA TCTCTTCAAT GTGATCTTCC GTATCTTCGA CAACATGAAA 
TTGCCGGAGC ACGTCACCGA GAAGTCCGAA TGGATGACGA CCACATGCAA CCACGCCTTG 
TACGCTATTA TTGATGTCTT CACGCAGTAT TTCGATGTTC TTGGTCATCT GCTGCTGGAG 
GAGCTCTTCG CCCAGCTGCA TTGGTGTGTT CAGCAGAGTA ACGAGCAGTT GGCGCGATCT 
GGCACCAATT GCCTGGAGAA CCTCGTCATT TCGAATGGAT TCAAGTTCAA CGAGTCCACC 
TGGGACAAGA CGTGCCAGTG CATCCTGGAC ATCTTCAACG CCACTTTGCC GCAGGATCTC 
CTCAGTTGGC GGCCGAAAGC ACATTCCAGT AACAATATAC CCCAGGAGCA CAACCACTTT 
GAGGCGCTGC ATATCCGCTG CGTAGTCCAG CTGGAACTGA TACAGACCAT GGATAACATT 
GTCTTTTTCC CGGCCACGTC GCGCAAGGAG GATGCCGAAA CGCTGGCCCA GGCGGCGGCA 
GACTTAACAG GCGGCAGGAG CGGTTCGCAG TCGCAGCTGC TGGAGTGCCA GCGGGAGGAG 
CAGGGAATGT ACGGCTATCT GAGAACCCGC CAGCTGCTCA CCCTGGCCGA CTGTCTGATG 
CAGTCGCACC GTTTTGCCAA GCGCTTCAAC GCCGATCACG ACCAACGCAG CCTGCTTTGG 
CGGGCGGGAT TCAAGGGATC TGTTAAACCG AATCTGCTGA AGCAGGAGAC CTCGTCGCTG 
GCCTGCGTCC TGCGCATTTT CTTCAAGATG TACGGCGACG AGAATAGACG CAGCGATTGG 
CCCGGCATCG AGCAGGAACT GGTGCAGGTC TGCAAGGAGG CACTGGGCTA CTATTTGAGT 
TTGCAGAGCG AGGCACACCG AGATGCGTGG ACATCGCTGC TGCTGCTCAT CCTGACGCGC 
CTGCTCAAGA TGTCCGATGC CAGGTTCGCC ACCCACGTTT CCAACTACTA CAGCCTGCTG 
TGCGAGATGA TGTGCTTCGA CCTCAAGCCC GAACTGAGAA GTGTCCTTAG GCGTGTGTTC 
ATGCGCATCG GTCCAGTATT CAATATAATG AGCGTTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec71 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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