AT3G60860 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT3G60860
Entrez Gene ID	825257
Full Name	SEC7-like guanine nucleotide exchange family protein
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_115950.4	NP_191645.1	SEC7-like guanine nucleotide exchange family protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT3G60860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT3G60860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb13984	NM_115950.4 Latest version!	Arabidopsis thaliana SEC7-like guanine nucleotide exchange family protein (AT3G60860), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT3G60860 ( NM_115950.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_191645.1 Arabidopsis thaliana SEC7-like guanine nucleotide exchange family protein (AT3G60860), mRNA.

Price & Availability↑

CloneID	OAb13984
Clone ID Related Accession (Same CDS sequence)	NM_115950.4
Accession Version	NM_115950.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	SEC7-like guanine nucleotide exchange family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_115950.3.

ATGGCTTCCT CGGAAGCTGA TTCCCGATTG AGTCGAGTCG TAACACCAGC TTTAGAGAAG 
ATCGTCAAGA ACGCGTCGTG GCGTAAACAC TCGAAGCTAG CTAACGAATG CAAAGCTGTT 
ATCGAGCGGC TAAATTCGCT GCAGAAATCA CCACCTCCTT CGTCCTCTGC TGCTACTGAC 
TCCGAGTCTG AATCCTCTGT TCCCGGTCCA CTTAACGACG GTGGTTCGAT CGAGTACTCG 
CTCGCCGATT CGGAACTCAT CTTCAGCCCT CTGATCAATG CCTGTGGCAC TGGCCTCGCC 
AAGATCATCG AACCTGCTAT TGACTGCATT CAGAAACTTA TCGCGCATGG TTACATTCGT 
GGTGAATCGG ACCCGTCCGG TGGAGCGGAG TCCCTTCTCC TTTTTAAATT GATCGATTCC 
GTTTGTAAAT GCCATGATTT GGGAGATGAA TCCATTGAAT TACCTGTGTT AAAGACTCTG 
TTATCTGCCA TTAACTCCAT CTCGCTGAGG ATCCATGGCA AATGCTTGCT CCTAGTTGTT 
CGTACTTGCT ACGACATTTA CTTGGGAAGC AAAAACGTGG TGAATCAGAC GACTGCCAAG 
GCTTCGCTGA TCCAGATTCT GGTGATTGTT TTCCGGAGAA TGGAGGCGGA TTCGTCAACG 
GTTCCAATTC AGCCGATTGT AGTGGCTGAG CTCATGGAGC CACTCGAAAA ATCAGATGCG 
GATGGGACAA TGACGCAGTT TGTTCAGGGT TTCATCACCA AAATCATGCA AGATATTGAC 
GGAGTGTTGA ACCCGACAAT GTCAGGATCA GGATCAGGAT CAGGGTCAGG AGGCCAAGAT 
GGGGCATATG GGACTACCAC AGTGGAGACT ACGAATCCTA CGGATTTGTT GGACTCCACT 
GATAAGGACA TGTTGGACGC TAAGTACTGG GAGATCAGTA TGTACAAGTC AGCTCTAGAG 
GGAAGGAAGG GGGAGCTAAC TGACGGAGAC GCTGAGAGAG ATGATGATTT AGAAGTTCAG 
ATAGAGAATA AGCTGCGGAG AGATGCCTGC CTGGTTTTCA GGGCGCTTTG TAAGCTCTCC 
ATGAAGGCGC CGCCAAAGGA GTCTTCGGCA GATCCGCAGT CGATGAGAGG GAAGATTCTG 
GCTCTTGAGC TGTTAAAGAT CTTGTTGGAA AATGCTGGAG CTGTCTTTAG AACAAGCGAG 
AAGTTTTCAG CTGATATCAA ACAGTTTTTG TGTTTGTCGT TGTTGAAGAA CAGTGCTTCT 
ACTCTTATGA TTATCTTCCA GCTTTCTTGC TCTATTTTCA TCAGTCTGGT TGCAAGATTC 
CGGGCTGGAT TAAAGGCCGA AATTGGAGTG TTTTTTCCAA TGATTGTTCT TAGAGTCGTG 
GAGAATGTAG CTCAACCTAA TTTTCAACAA AAGATGATTG TTCTTCGGTT TCTTGACAAA 
CTTTGTTTGG ATTCACAAAT CCTAGTTGAT ATATTCTTGA ACTACGATTG CGATGTCAAT 
TCATCAAATA TCTTTGAGAG GATGGTCAAT GGTCTCCTTA AAACGGCTCA AGGGGTTCCT 
CCTGGTACAG CTACAACATT GATGCCACCA CAGGAGGCAG CTATGAAACT TGAAGCCATG 
AAATGCTTAG TGGCCATATT GAAATCAATG GGAGATTGGT TGAACAAGCA GTTGCGCCTG 
CCAGTTTCTA ATTCATTGAA TAAATCTGAC GTAATTGAGA TTGATTTGGG ACCCGGAAGT 
CCCCAGTTGG CAAATGGAAA TGCTGATGAG TCTGCTGACG GATCAGATAC TTATTCTGAA 
TCTTCAGGAG GTACATCTGA TGCTCTAGCT ATCGAGCAGC GCCGGGCTTA CAAACTCGAG 
CTTCAGGAAG GTATTTCTCT TTTTAATCGG AAGCCAACTA AAGGTATTGA GTTTCTCATC 
AATGCGGGTA AGGTGGGTGA ATCTCCTGAA GAAATAGCAG GTTTTCTCAA AGACGCGTCA 
GGGTTAAATA AAACTTTGAT AGGCGACTAT CTTGGAGAAA GAGAAGATTT AGCTCTCAAG 
GTGATGCATG CATATGTGGA TTCTTTTGAT TTCCGAGGCA TGGAATTTGA TGAGGCAATT 
AGAACCTTTC TTGAAGGTTT TAGGCTGCCA GGAGAAGCAC AAAAGATTGA CCGAATCATG 
GAAAAGTTTG CTGAACGTTA CTGCAAGTGT AATCCTAAGG TGTTTACTAG CGCAGATTCA 
GCTTATGTTC TTGCATATTC TGTGATCATG CTCAATACTG ATGCCCACAA CCCCATGGTG 
AAGAACAAGA TGTCTGCTGA TGACTTTATC AGAAACAATC GTGGAATAGA CGACGGAAAA 
GATTTACCTG CGGACTACAT GAGATCGCTG TATGAAAGGA TAACAAAGCA TGAGATAAAG 
ATGAAAGAAG ATGATTTACG TTTGCAACAG AAACAGTACG CAAACTCAAA CAGAATGCTT 
GGTTTGGATG GTATATTAAA CATAGTGATC CGTAAGCAGT GGGGAGATTC ATATGCGGAA 
ACAAGTGATG ATCTGATGAA GCATATGCAA GAGCAATTTA AGGAAAAAGC TCGTAAATCA 
GAGTCAACCT ACTATGCTGC TACAGATGTG GTTATTCTCA GATTTATGAT TGAGGCTTGT 
TGGGCTCCTA TGCTTGCTGC ATTCAGTGTG CCGCTAGACC AGAGTGATGA TTTGATAGTG 
ATCAACATAT GTCTTGAAGG CTTCCACCAT GCAATCCATG CCACTTCGTT GATGTCAATG 
AAGACCCATA GAGATGCTTT TGTGACTTCA CTAGCGAAGT TCACTTCACT TCACTCCCCA 
GCTGATATCA AGCAGAGAAA TATTGAAGCC ATTAAGGCGA TTCTCAGACT TGCTGATGAA 
GAGGGCAACT ATTTGCAAGA TGCCTGGGAG CATATACTAA CTTGTGTTTC CCGTTTTGAG 
CAACTACATC TCTTGGGAGA AGGTGCTCCT CCAGATGCCA CTTTTTTTGC TTCCAAGCAA 
AATGAGTCAG AGAAATCAAA GCAACCCAAA CAGTATATAC TTCCTGTTCT GAAAAGGAAG 
GGTCCTGGGA AAAGTCAATA TGCAGCTACA GGTGTCTTGA GGGGTTCATA TGACAGCATG 
AGTCTTGGCG GAAAAGGTTC TAAGAACGTG AGACAAGAAC AGATGAGCAG TATAGTCTCA 
AATTTGAACT TGTTGGAGCA GGTTGGTGAA ATGAATCAAG TATTTTCTCA GAGTCAGAAA 
TTAAACAGTG AAGCTATAAT AGACTTTGTT AAAGCTCTCT GCAAAGTTTC TATGGATGAG 
TTGCGGTCTC CTTCTAATCC ACGCGTTTTT AGCCTCACAA AGATTGTTGA GATAGCGCAT 
TATAACATGA ATCGCATAAG GCTTGTTTGG TCAAGCATCT GGCAAGTGCT ATCAGGTTTC 
TTTGTGACCA TTGGATGCTC AGAGAATCTT TCTATTGCAA TATTTGCAAT GGATTCTCTC 
CGCCAGTTGT CAATGAAATT TCTGGAACGG GAGGAGTTGG CTAATTACAA CTTCCAAAAT 
GAATTTATGA CACCTTTTGT CATTGTTATG CGTAGAAGTA ATGATGTTGA GATCAGAGAA 
CTTATCATCC GATGTGTTTC TCAGATGGTA TTGTCACGTG TAAATAATGT TAAGTCAGGA 
TGGAAAAGCA TGTTTATGGT TTTTACCACA GCGGCATATG ACGACCACAA AAATATTGTG 
TTTTTGTCCT TTGAAATTAT CGAGAAGATT ATTCGAGAAT ATTTCCCATA CATCACTGAG 
ACAGAAACCA CCACTTTTAC AGATTGTGTG AATTGCCTAG TTGCATTCAC CAATAACAGA 
TTTAGCAAGG ATATCAGTCT CAGTTCTATA GCTTTCCTCC GGTATTGTGC AACAAAGCTT 
GCAGAAGGAG ATCTTAACTC GCCATCAACA AACAAATACA AGGGAACTTC TGGAAAGATT 
CCTCAGTCAT CTCTCCACAG TGGAAAAAGT GGGAAACAAG AGAACGGAGA GATTGTAAAC 
AACAATCATC TTTATTTTTG GTTTCCCTTA CTATCAGGTT TATCAGAGCT TAGTTTCGAT 
CCAAGACCAG AAATCAGGAA AAGCGCACTA CAGATTATGT TTGATACCTT ACGCAACCAT 
GGCCATTTAT TTTCTCTGCC GTTATGGGAA AAAGTATTCG AGTCTGTATT ATTTCCTATA 
TTCGACTACG TACGCCATTC TATTGATCCT TCTGGCGAAG ACGAATCTGC TGATCAAGGA 
AGTTCTGGTG GTGAAGTGGA TGAGCTAGAT CATGACGCCT GGCTCTACGA GACCTGTACA 
TTAGCACTAC AGCTTGTAGT GGATCTCTTT GTCAAATTTT ACACTACAGT CAATCCACTT 
CTAGAGAAAG TACTAATGCT TCTTGTAAGC TTCATAAAGA GACCTCACCA GAGCCTTGCA 
GGAATTGGTA TTGCTGCGTT TGTTCGTCTG ATGAGCGATG CTGATGGCTT GTTCTCTGAA 
GAAAAGTGGT TAGAGGTGGT GTCAGCTTTA AAAGAAGCGG CGAAAACAAC TTGCCCAGAT 
TTTTCGTATT TCCTTAGCGA AGAATATGTA GCAAGAAGCC AGAGAAGTGC TCTCAACATT 
CAGAACAGTA ATGCTGAGTC TGCTGCTCCA ACTGCTACCG ATGGCAATGA AGAGAGTCAG 
AGAACAGCAA CCCATCTTTA CGCTGCTATC TCTGATGCTA AGTGCCGAGC CGCTGTTCAA 
CTTTTGTTGA TCCAGGCTGT GATGGAGATT TACAACATGT ACAGACCTCA GCTTTCAGCG 
AAAAACACAT TAGTCCTCGT AGATGCATTG CACGGTGTAG CTTTACATGC TCACGGAATA 
AACAGTAACA CAATACTTCG TTCAAGACTA CAAGAGCTAG GACCCATGAC ACAGATGCAA 
GATCCTCCAC TACTTCGTCT TGAAAACGAG TCTTACCAAA TCTGCCTGAC ATTCTTACAG 
AATCTTGTTG CAGACAAAAC CAAAAAAGAA GAAGAAGAAG AAGAAGAGGA AATAGAATCA 
CTTCTAGTCA ACATCTGTCA AGAAGTTCTC AACTTCTACA TCGAGACTTC TTCTTCTGCG 
AAAAAATTAC AATCAGAATC ATCACGGGCC AGCGAATACC GATGGAGAAT ACCTTTGGGA 
TCAGGAAAAC GAAGAGAACT ATCAGCAAGA GCTCCTCTTA TAGTTGCAAC ACTTCAAGCA 
ATGTGTACAT TGGATGAGGC ATCATTTGAG AAGAACCTGA AATGTCTATT TCCACTTCTG 
GCTAACTTGA TTAGCTGCGA ACACGGCTCT AACGAGGTTC AGACTGCTCT TGCTGACATG 
CTTGGCCTAT CGGTTGGCCC GGTTTTACTC CAATGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_191645.1
CDS	408..5789
Translation	MASSEADSRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGESDPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKAPPKESSADPQSMRGKILALELLKILLENAGAVFRTSEKFSADIKQFLCLSLLKNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVLRFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAMKLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGSDTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGFLKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQKIDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVIRKQWGDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQSDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAILRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYILPVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVGEMNQVFSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSNDVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREYFPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYKGTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMFDTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQGSSGGEVDELDHDAWLYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDADGLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALNIQNSNAESAAPTATDGNEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVALHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFLQNLVADKTKKEEEEEEEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPLGSGKRRELSARAPLIVATLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQTALADMLGLSVGPVLLQWC

Target ORF information:

RefSeq Version	NM_115950.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana SEC7-like guanine nucleotide exchange family protein (AT3G60860), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_115950.4

ORF Insert Sequence:

ATGGCTTCCT CGGAAGCTGA TTCCCGATTG AGTCGAGTCG TAACACCAGC TTTAGAGAAG 
ATCGTCAAGA ACGCGTCGTG GCGTAAACAC TCGAAGCTAG CTAACGAATG CAAAGCTGTT 
ATCGAGCGGC TAAATTCGCT GCAGAAATCA CCACCTCCTT CGTCCTCTGC TGCTACTGAC 
TCCGAGTCTG AATCCTCTGT TCCCGGTCCA CTTAACGACG GTGGTTCGAT CGAGTACTCG 
CTCGCCGATT CGGAACTCAT CTTCAGCCCT CTGATCAATG CCTGTGGCAC TGGCCTCGCC 
AAGATCATCG AACCTGCTAT TGACTGCATT CAGAAACTTA TCGCGCATGG TTACATTCGT 
GGTGAATCGG ACCCGTCCGG TGGAGCGGAG TCCCTTCTCC TTTTTAAATT GATCGATTCC 
GTTTGTAAAT GCCATGATTT GGGAGATGAA TCCATTGAAT TACCTGTGTT AAAGACTCTG 
TTATCTGCCA TTAACTCCAT CTCGCTGAGG ATCCATGGCA AATGCTTGCT CCTAGTTGTT 
CGTACTTGCT ACGACATTTA CTTGGGAAGC AAAAACGTGG TGAATCAGAC GACTGCCAAG 
GCTTCGCTGA TCCAGATTCT GGTGATTGTT TTCCGGAGAA TGGAGGCGGA TTCGTCAACG 
GTTCCAATTC AGCCGATTGT AGTGGCTGAG CTCATGGAGC CACTCGAAAA ATCAGATGCG 
GATGGGACAA TGACGCAGTT TGTTCAGGGT TTCATCACCA AAATCATGCA AGATATTGAC 
GGAGTGTTGA ACCCGACAAT GTCAGGATCA GGATCAGGAT CAGGGTCAGG AGGCCAAGAT 
GGGGCATATG GGACTACCAC AGTGGAGACT ACGAATCCTA CGGATTTGTT GGACTCCACT 
GATAAGGACA TGTTGGACGC TAAGTACTGG GAGATCAGTA TGTACAAGTC AGCTCTAGAG 
GGAAGGAAGG GGGAGCTAAC TGACGGAGAC GCTGAGAGAG ATGATGATTT AGAAGTTCAG 
ATAGAGAATA AGCTGCGGAG AGATGCCTGC CTGGTTTTCA GGGCGCTTTG TAAGCTCTCC 
ATGAAGGCGC CGCCAAAGGA GTCTTCGGCA GATCCGCAGT CGATGAGAGG GAAGATTCTG 
GCTCTTGAGC TGTTAAAGAT CTTGTTGGAA AATGCTGGAG CTGTCTTTAG AACAAGCGAG 
AAGTTTTCAG CTGATATCAA ACAGTTTTTG TGTTTGTCGT TGTTGAAGAA CAGTGCTTCT 
ACTCTTATGA TTATCTTCCA GCTTTCTTGC TCTATTTTCA TCAGTCTGGT TGCAAGATTC 
CGGGCTGGAT TAAAGGCCGA AATTGGAGTG TTTTTTCCAA TGATTGTTCT TAGAGTCGTG 
GAGAATGTAG CTCAACCTAA TTTTCAACAA AAGATGATTG TTCTTCGGTT TCTTGACAAA 
CTTTGTTTGG ATTCACAAAT CCTAGTTGAT ATATTCTTGA ACTACGATTG CGATGTCAAT 
TCATCAAATA TCTTTGAGAG GATGGTCAAT GGTCTCCTTA AAACGGCTCA AGGGGTTCCT 
CCTGGTACAG CTACAACATT GATGCCACCA CAGGAGGCAG CTATGAAACT TGAAGCCATG 
AAATGCTTAG TGGCCATATT GAAATCAATG GGAGATTGGT TGAACAAGCA GTTGCGCCTG 
CCAGTTTCTA ATTCATTGAA TAAATCTGAC GTAATTGAGA TTGATTTGGG ACCCGGAAGT 
CCCCAGTTGG CAAATGGAAA TGCTGATGAG TCTGCTGACG GATCAGATAC TTATTCTGAA 
TCTTCAGGAG GTACATCTGA TGCTCTAGCT ATCGAGCAGC GCCGGGCTTA CAAACTCGAG 
CTTCAGGAAG GTATTTCTCT TTTTAATCGG AAGCCAACTA AAGGTATTGA GTTTCTCATC 
AATGCGGGTA AGGTGGGTGA ATCTCCTGAA GAAATAGCAG GTTTTCTCAA AGACGCGTCA 
GGGTTAAATA AAACTTTGAT AGGCGACTAT CTTGGAGAAA GAGAAGATTT AGCTCTCAAG 
GTGATGCATG CATATGTGGA TTCTTTTGAT TTCCGAGGCA TGGAATTTGA TGAGGCAATT 
AGAACCTTTC TTGAAGGTTT TAGGCTGCCA GGAGAAGCAC AAAAGATTGA CCGAATCATG 
GAAAAGTTTG CTGAACGTTA CTGCAAGTGT AATCCTAAGG TGTTTACTAG CGCAGATTCA 
GCTTATGTTC TTGCATATTC TGTGATCATG CTCAATACTG ATGCCCACAA CCCCATGGTG 
AAGAACAAGA TGTCTGCTGA TGACTTTATC AGAAACAATC GTGGAATAGA CGACGGAAAA 
GATTTACCTG CGGACTACAT GAGATCGCTG TATGAAAGGA TAACAAAGCA TGAGATAAAG 
ATGAAAGAAG ATGATTTACG TTTGCAACAG AAACAGTACG CAAACTCAAA CAGAATGCTT 
GGTTTGGATG GTATATTAAA CATAGTGATC CGTAAGCAGT GGGGAGATTC ATATGCGGAA 
ACAAGTGATG ATCTGATGAA GCATATGCAA GAGCAATTTA AGGAAAAAGC TCGTAAATCA 
GAGTCAACCT ACTATGCTGC TACAGATGTG GTTATTCTCA GATTTATGAT TGAGGCTTGT 
TGGGCTCCTA TGCTTGCTGC ATTCAGTGTG CCGCTAGACC AGAGTGATGA TTTGATAGTG 
ATCAACATAT GTCTTGAAGG CTTCCACCAT GCAATCCATG CCACTTCGTT GATGTCAATG 
AAGACCCATA GAGATGCTTT TGTGACTTCA CTAGCGAAGT TCACTTCACT TCACTCCCCA 
GCTGATATCA AGCAGAGAAA TATTGAAGCC ATTAAGGCGA TTCTCAGACT TGCTGATGAA 
GAGGGCAACT ATTTGCAAGA TGCCTGGGAG CATATACTAA CTTGTGTTTC CCGTTTTGAG 
CAACTACATC TCTTGGGAGA AGGTGCTCCT CCAGATGCCA CTTTTTTTGC TTCCAAGCAA 
AATGAGTCAG AGAAATCAAA GCAACCCAAA CAGTATATAC TTCCTGTTCT GAAAAGGAAG 
GGTCCTGGGA AAAGTCAATA TGCAGCTACA GGTGTCTTGA GGGGTTCATA TGACAGCATG 
AGTCTTGGCG GAAAAGGTTC TAAGAACGTG AGACAAGAAC AGATGAGCAG TATAGTCTCA 
AATTTGAACT TGTTGGAGCA GGTTGGTGAA ATGAATCAAG TATTTTCTCA GAGTCAGAAA 
TTAAACAGTG AAGCTATAAT AGACTTTGTT AAAGCTCTCT GCAAAGTTTC TATGGATGAG 
TTGCGGTCTC CTTCTAATCC ACGCGTTTTT AGCCTCACAA AGATTGTTGA GATAGCGCAT 
TATAACATGA ATCGCATAAG GCTTGTTTGG TCAAGCATCT GGCAAGTGCT ATCAGGTTTC 
TTTGTGACCA TTGGATGCTC AGAGAATCTT TCTATTGCAA TATTTGCAAT GGATTCTCTC 
CGCCAGTTGT CAATGAAATT TCTGGAACGG GAGGAGTTGG CTAATTACAA CTTCCAAAAT 
GAATTTATGA CACCTTTTGT CATTGTTATG CGTAGAAGTA ATGATGTTGA GATCAGAGAA 
CTTATCATCC GATGTGTTTC TCAGATGGTA TTGTCACGTG TAAATAATGT TAAGTCAGGA 
TGGAAAAGCA TGTTTATGGT TTTTACCACA GCGGCATATG ACGACCACAA AAATATTGTG 
TTTTTGTCCT TTGAAATTAT CGAGAAGATT ATTCGAGAAT ATTTCCCATA CATCACTGAG 
ACAGAAACCA CCACTTTTAC AGATTGTGTG AATTGCCTAG TTGCATTCAC CAATAACAGA 
TTTAGCAAGG ATATCAGTCT CAGTTCTATA GCTTTCCTCC GGTATTGTGC AACAAAGCTT 
GCAGAAGGAG ATCTTAACTC GCCATCAACA AACAAATACA AGGGAACTTC TGGAAAGATT 
CCTCAGTCAT CTCTCCACAG TGGAAAAAGT GGGAAACAAG AGAACGGAGA GATTGTAAAC 
AACAATCATC TTTATTTTTG GTTTCCCTTA CTATCAGGTT TATCAGAGCT TAGTTTCGAT 
CCAAGACCAG AAATCAGGAA AAGCGCACTA CAGATTATGT TTGATACCTT ACGCAACCAT 
GGCCATTTAT TTTCTCTGCC GTTATGGGAA AAAGTATTCG AGTCTGTATT ATTTCCTATA 
TTCGACTACG TACGCCATTC TATTGATCCT TCTGGCGAAG ACGAATCTGC TGATCAAGGA 
AGTTCTGGTG GTGAAGTGGA TGAGCTAGAT CATGACGCCT GGCTCTACGA GACCTGTACA 
TTAGCACTAC AGCTTGTAGT GGATCTCTTT GTCAAATTTT ACACTACAGT CAATCCACTT 
CTAGAGAAAG TACTAATGCT TCTTGTAAGC TTCATAAAGA GACCTCACCA GAGCCTTGCA 
GGAATTGGTA TTGCTGCGTT TGTTCGTCTG ATGAGCGATG CTGATGGCTT GTTCTCTGAA 
GAAAAGTGGT TAGAGGTGGT GTCAGCTTTA AAAGAAGCGG CGAAAACAAC TTGCCCAGAT 
TTTTCGTATT TCCTTAGCGA AGAATATGTA GCAAGAAGCC AGAGAAGTGC TCTCAACATT 
CAGAACAGTA ATGCTGAGTC TGCTGCTCCA ACTGCTACCG ATGGCAATGA AGAGAGTCAG 
AGAACAGCAA CCCATCTTTA CGCTGCTATC TCTGATGCTA AGTGCCGAGC CGCTGTTCAA 
CTTTTGTTGA TCCAGGCTGT GATGGAGATT TACAACATGT ACAGACCTCA GCTTTCAGCG 
AAAAACACAT TAGTCCTCGT AGATGCATTG CACGGTGTAG CTTTACATGC TCACGGAATA 
AACAGTAACA CAATACTTCG TTCAAGACTA CAAGAGCTAG GACCCATGAC ACAGATGCAA 
GATCCTCCAC TACTTCGTCT TGAAAACGAG TCTTACCAAA TCTGCCTGAC ATTCTTACAG 
AATCTTGTTG CAGACAAAAC CAAAAAAGAA GAAGAAGAAG AAGAAGAGGA AATAGAATCA 
CTTCTAGTCA ACATCTGTCA AGAAGTTCTC AACTTCTACA TCGAGACTTC TTCTTCTGCG 
AAAAAATTAC AATCAGAATC ATCACGGGCC AGCGAATACC GATGGAGAAT ACCTTTGGGA 
TCAGGAAAAC GAAGAGAACT ATCAGCAAGA GCTCCTCTTA TAGTTGCAAC ACTTCAAGCA 
ATGTGTACAT TGGATGAGGC ATCATTTGAG AAGAACCTGA AATGTCTATT TCCACTTCTG 
GCTAACTTGA TTAGCTGCGA ACACGGCTCT AACGAGGTTC AGACTGCTCT TGCTGACATG 
CTTGGCCTAT CGGTTGGCCC GGTTTTACTC CAATGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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AT3G60860 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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