arfgef1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arfgef1
Entrez Gene ID	100329953
Full Name	ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Synonyms	hm:zeh1651,im:7138204,wu:fj64h08
General protein information	Preferred Names ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 zeh1651
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297395.1	XP_009295670.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
XM_009297398.1	XP_009295673.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X5
XM_009297397.1	XP_009295672.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X4
XM_009297396.1	XP_009295671.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
XM_009297394.1	XP_009295669.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
XM_009297399.1	XP_009295674.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X6

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology

The following arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49262	XM_009297394.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49263	XM_009297395.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49264	XM_009297396.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49265	XM_009297397.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49266	XM_009297398.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49267	XM_009297399.1 Latest version!	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arfgef1 ( XM_009297394.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295669.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49262
Clone ID Related Accession (Same CDS sequence)	XM_009297394.1
Accession Version	XM_009297394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA 
AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC 
AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT 
AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG 
CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC 
GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA 
CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC 
AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG 
ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC 
GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG 
TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG 
TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC 
ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC 
CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA 
CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA 
TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC 
AACCCCAATT CCCAGACAAG TCTTGGGAGA GCAGGCCAAG AAAAGCCTTC AGAGCAGGAG 
AGCAATGAGT CGAAGCATCC AGAAACGATC AACCGTTACG GCAGCATTAA CTCTTTGGAC 
TCCACTGCAT CATCAGGCAT TGGGAGCTAC AGCACACAGA TGTCCGGAAC AGACAACCCG 
GAGCAGTTTG AGGTCCTCAA ACAACAGAAG GAGATCATAG AGCAAGGCAT TGACCTGTTC 
AACAAAAAAC CAAAGAGGGG CATTCAGTAC CTGCAGGAGC AAGGCATGCT GGGAACAACG 
CCTGAAGATA TTGCCCAGTT TTTACATCAA GAGGAGAGAC TTGACTCTAT CCAGGTAGGA 
GAGTTTCTAG GAGACAACGA CCGCATCAAT AAAGAGGTCA TGTATGCCTA CGTCGACCAA 
ATGGACTTCC AGGGCAAAGA CTTCGTGCCA GCGCTGCGCA TGTTCCTGGA GGGTTTCCGT 
CTGCCCGGCG AAGCCCAGAA AATTGATCGA CTGATGGAGA AATTTGCTGC TAGATATTTA 
GAATGCAACC AAGGGCAAAC ACTGTTCGCC AGCGCTGACA CCGCATACGT TCTTGCGTAT 
TCAATCATCA TGTTGACTAC CGATCTGCAC AGTCCACAGG TGAAGAATAA AATGACGAAA 
GAGCAATACA TCAAGATGAA TCGAGGTATC AATGACAGTA AAGATTTACC GGAGGAGTAT 
CTCTCAGCCA TTTATGATGA AATCGCAGGG AAGAAAATCT CCATGAAGGA GACAAAGGAG 
TTAACTCTCA AATCAAATAA GCAAAGTGTG GCGAGTGAGA AGCAGAGGAG GCTGCTGTAT 
AATGTTGAGA TGGAGCAGAT GGCAAAAACA GCAAAGGCTC TGATGGAGGC CGTGAGCCAC 
GTTCAAGCCC CCTTCACCAG CGCCACACAT CTTGAGCATG TCAGACCAAT GTTTAAGCTG 
GCCTGGACGC CCTTTCTGGC TGCTTTCAGT GTGGGTCTGC AGGACTGTGA TGACACTGAA 
GTGGCCTCTC TGTGTCTGGA GGGAATCCGC TGTGCCATCA GGATAGCATG CATCTTCAGC 
ATTCAGTTGG AGCGAGACGC ATATGTACAG GCTCTGGCCC GTTTCACACT CTTGACGGCC 
AGTTCTGGCA TCACTGAAAT GAAGCAGAAG AACATCGACA CCATCAAGAC CCTCATCACA 
GTGGCTCACA CTGATGGAAA TTATCTAGGA AACTCCTGGC ATGAGATCAT GAAGTGCATT 
AGTCAGCTGG AGTTGGCCCA GCTCATCGGG ACAGGAGTGA AGGCTCGGTA CATATCAGGA 
ACTGTTCGGG GAAAGGAAGG CTTTATCACC AGCACCAAGG AACAAACGTC TGATGAATAC 
CTGGGATTGG GAGGGAATGT TGATCGCAAA CAGATTGCAA GCATTCAGGA GTCTATCGGG 
GAGACCAGCT CGCAAAGTGT AGTTGTTGCT GTAGACCGGA TATTCACGGG CTCTACCAGA 
CTTGATGGAA ATGCCATTGT GGACTTTGTG CGATGGCTGT GCGCTGTCTC GATGGATGAA 
CTGGCCTCCC CAACACACCC TCGAATGTTC AGTCTGCAGA AGATCGTGGA GATTTCATAC 
TACAATATGG GTCGTATCAG GTTGCAGTGG TCCAGGATTT GGGAGGTTAT AGGGGATCAT 
TTCAATAAGG TTGGCTGTAA TCCCAACGAA GATGTTGCTA TTTTTGCTGT GGATTCTCTG 
AGGCAGTTGT CCATGAAGTT TTTGGAAAAG GGAGAGTTGG CCAATTTCAG ATTCCAGAAG 
GATTTCCTCA GGCCGTTCGA GCACATAATG AAAAAGAACA GATCTCCGAC CATTAGAGAC 
ATGGTGGTTC GCTGTATTGC CCAGATGGTC AACTCTCAGG CTGGCAATAT TAGATCAGGG 
TGGAAGAACA TTTTCTCTGT GTTTCATCTG GCTGCTTCTG ATCAGGATGA GAGTATAGTT 
GAGCTGGCCT TCCAGACAAC TGGACACATC GTCACGAATG TATTTGAGAA GCATTTCCCA 
GCTACCATTG ACTCCTTCCA GGACGCTGTC AAGTGTTTGT CTGAGTTCGC CTGCAACGCT 
TCTTTTCCAG ACACCAGCAT GGAGGCTATT CGTCTGATCC GCCACTGCGC CAAATATGTG 
TCAGACCGAC CCCAGGCGTT TAAAGACTAT ACCAGTGATG ATATGAATGT TGCCCCGGAG 
GACCGTGTTT GGGTCAGAGG CTGGTTCCCC ATCCTTTTTG AGCTGTCATG CATCATCAAC 
AGATGCAAAC TGGACGTCCG GACCAGGGGT CTGACAGTGA TGTTTGAGGT GATGAAGACG 
TACGGTCATA CCTATGAGAA ACACTGGTGG CAGGATCTGT TCCGCATCGT CTTCAGGATC 
TTCGACAACA TGAAGCTTCC TGAGCAACAG ACCGAGAAAG CGGAGTGGAT GACCACCACC 
TGCAATCATG CACTTTATGC CATCTGTGAC GTTTTTACAC AGTACTTTGA GTCTCTCAGT 
GATGTTCTTT TAGATGATAT CTTATCTCAG CTCTACTGGT GTGTCCAGCA AGATAATGAG 
CAATTAGCCA GATCAGGCAC TAACTGCCTT GAAAACGTGG TCATCCTTAA TGGTGAGAAG 
TTCAATCCTG AGACTTGGGA CAAGACATGC AACTGCATGC TGGACATCTT CAAAACCACC 
ATCCCACACA TGCTGCTGAC TTGGAGACCG GCTGGTGCTG AAGGAGATCA CATGACTCAG 
CTGGAGTCTG ATAAACAGCT GGACTCCATT TCCCAGAAGT CAGTGGATAT TCAGACCCGC 
TCAGATGACC AGCAGTCTGT TAACAGTATG GAGAAGGCTT TGGCTGACAA CCGCAGATAC 
AGTCAGTTCA GTGTGGCTTC AGAAGCTCAG GAACAGAGGC TGTTTGCTGC GCTACTCATT 
AAGTGTGTGG TTCAGCTGGA GCTCATTCAG ACCATTGACA ACATCGTCTT CTTTCCTGCT 
ACCAGTAAGA AAGAAGATGC GGAAAACTTT GCGGCTGCAC AGAGAGATGC TTTAGATGCT 
GATATACATG TGGACACACA GGACCAGGGA ATGTATCGCT ATCTGACCTC AGAACAGCTC 
TTCAAACTGC TCGACTGTCT GCTGGAGTCT CATCGATTTG CAAAGGCCTT CAACTCCAAT 
AATGAGCAAA GAACTGCTCT GTGGAAAGCC GGTTTCAAAG GGAAGTCGAA GCCCAATCTG 
CTGAAACAGG AGACGAGCAG CCTGGCGTGT GGTCTGCGCA TCCTCTTCCG CATGTACACA 
GATCCAAGCC GACGGGATGC CTGGGAGGAG GTGCAGAGGA GACTGCTCAA CGTTTGCAGT 
GACGCAGTGG CTTATTTTCT GACCCTCACG TCTGAAAGTC ATCGCGAAGC CTGGACAAAC 
CTTCTGCTGC TCTTCCTGAC CAAAGTGCTG AAGATCAGTG ATGATAGATT CAAGGCTCAT 
GCGTCCCGCT ACTACCCTCT GCTCTGTGAA ATCATGCAGT TCGACCTGAT CCCGGAGCTG 
CGGGCCGTCC TGCGCAAGTT TTTCCTGCGC ATTGGACTGG TTTTCCAGAT CACCAACAGC 
CTGGAAACAA ATCAAACTGT CAGCAACGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295669.1
CDS	206..5818
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALTNHKISWSLASRKRDRQLQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATGNMYVTEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGRAGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297394.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297394.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA 
AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC 
AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT 
AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG 
CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC 
GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA 
CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC 
AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG 
ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC 
GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG 
TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG 
TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC 
ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC 
CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA 
CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA 
TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC 
AACCCCAATT CCCAGACAAG TCTTGGGAGA GCAGGCCAAG AAAAGCCTTC AGAGCAGGAG 
AGCAATGAGT CGAAGCATCC AGAAACGATC AACCGTTACG GCAGCATTAA CTCTTTGGAC 
TCCACTGCAT CATCAGGCAT TGGGAGCTAC AGCACACAGA TGTCCGGAAC AGACAACCCG 
GAGCAGTTTG AGGTCCTCAA ACAACAGAAG GAGATCATAG AGCAAGGCAT TGACCTGTTC 
AACAAAAAAC CAAAGAGGGG CATTCAGTAC CTGCAGGAGC AAGGCATGCT GGGAACAACG 
CCTGAAGATA TTGCCCAGTT TTTACATCAA GAGGAGAGAC TTGACTCTAT CCAGGTAGGA 
GAGTTTCTAG GAGACAACGA CCGCATCAAT AAAGAGGTCA TGTATGCCTA CGTCGACCAA 
ATGGACTTCC AGGGCAAAGA CTTCGTGCCA GCGCTGCGCA TGTTCCTGGA GGGTTTCCGT 
CTGCCCGGCG AAGCCCAGAA AATTGATCGA CTGATGGAGA AATTTGCTGC TAGATATTTA 
GAATGCAACC AAGGGCAAAC ACTGTTCGCC AGCGCTGACA CCGCATACGT TCTTGCGTAT 
TCAATCATCA TGTTGACTAC CGATCTGCAC AGTCCACAGG TGAAGAATAA AATGACGAAA 
GAGCAATACA TCAAGATGAA TCGAGGTATC AATGACAGTA AAGATTTACC GGAGGAGTAT 
CTCTCAGCCA TTTATGATGA AATCGCAGGG AAGAAAATCT CCATGAAGGA GACAAAGGAG 
TTAACTCTCA AATCAAATAA GCAAAGTGTG GCGAGTGAGA AGCAGAGGAG GCTGCTGTAT 
AATGTTGAGA TGGAGCAGAT GGCAAAAACA GCAAAGGCTC TGATGGAGGC CGTGAGCCAC 
GTTCAAGCCC CCTTCACCAG CGCCACACAT CTTGAGCATG TCAGACCAAT GTTTAAGCTG 
GCCTGGACGC CCTTTCTGGC TGCTTTCAGT GTGGGTCTGC AGGACTGTGA TGACACTGAA 
GTGGCCTCTC TGTGTCTGGA GGGAATCCGC TGTGCCATCA GGATAGCATG CATCTTCAGC 
ATTCAGTTGG AGCGAGACGC ATATGTACAG GCTCTGGCCC GTTTCACACT CTTGACGGCC 
AGTTCTGGCA TCACTGAAAT GAAGCAGAAG AACATCGACA CCATCAAGAC CCTCATCACA 
GTGGCTCACA CTGATGGAAA TTATCTAGGA AACTCCTGGC ATGAGATCAT GAAGTGCATT 
AGTCAGCTGG AGTTGGCCCA GCTCATCGGG ACAGGAGTGA AGGCTCGGTA CATATCAGGA 
ACTGTTCGGG GAAAGGAAGG CTTTATCACC AGCACCAAGG AACAAACGTC TGATGAATAC 
CTGGGATTGG GAGGGAATGT TGATCGCAAA CAGATTGCAA GCATTCAGGA GTCTATCGGG 
GAGACCAGCT CGCAAAGTGT AGTTGTTGCT GTAGACCGGA TATTCACGGG CTCTACCAGA 
CTTGATGGAA ATGCCATTGT GGACTTTGTG CGATGGCTGT GCGCTGTCTC GATGGATGAA 
CTGGCCTCCC CAACACACCC TCGAATGTTC AGTCTGCAGA AGATCGTGGA GATTTCATAC 
TACAATATGG GTCGTATCAG GTTGCAGTGG TCCAGGATTT GGGAGGTTAT AGGGGATCAT 
TTCAATAAGG TTGGCTGTAA TCCCAACGAA GATGTTGCTA TTTTTGCTGT GGATTCTCTG 
AGGCAGTTGT CCATGAAGTT TTTGGAAAAG GGAGAGTTGG CCAATTTCAG ATTCCAGAAG 
GATTTCCTCA GGCCGTTCGA GCACATAATG AAAAAGAACA GATCTCCGAC CATTAGAGAC 
ATGGTGGTTC GCTGTATTGC CCAGATGGTC AACTCTCAGG CTGGCAATAT TAGATCAGGG 
TGGAAGAACA TTTTCTCTGT GTTTCATCTG GCTGCTTCTG ATCAGGATGA GAGTATAGTT 
GAGCTGGCCT TCCAGACAAC TGGACACATC GTCACGAATG TATTTGAGAA GCATTTCCCA 
GCTACCATTG ACTCCTTCCA GGACGCTGTC AAGTGTTTGT CTGAGTTCGC CTGCAACGCT 
TCTTTTCCAG ACACCAGCAT GGAGGCTATT CGTCTGATCC GCCACTGCGC CAAATATGTG 
TCAGACCGAC CCCAGGCGTT TAAAGACTAT ACCAGTGATG ATATGAATGT TGCCCCGGAG 
GACCGTGTTT GGGTCAGAGG CTGGTTCCCC ATCCTTTTTG AGCTGTCATG CATCATCAAC 
AGATGCAAAC TGGACGTCCG GACCAGGGGT CTGACAGTGA TGTTTGAGGT GATGAAGACG 
TACGGTCATA CCTATGAGAA ACACTGGTGG CAGGATCTGT TCCGCATCGT CTTCAGGATC 
TTCGACAACA TGAAGCTTCC TGAGCAACAG ACCGAGAAAG CGGAGTGGAT GACCACCACC 
TGCAATCATG CACTTTATGC CATCTGTGAC GTTTTTACAC AGTACTTTGA GTCTCTCAGT 
GATGTTCTTT TAGATGATAT CTTATCTCAG CTCTACTGGT GTGTCCAGCA AGATAATGAG 
CAATTAGCCA GATCAGGCAC TAACTGCCTT GAAAACGTGG TCATCCTTAA TGGTGAGAAG 
TTCAATCCTG AGACTTGGGA CAAGACATGC AACTGCATGC TGGACATCTT CAAAACCACC 
ATCCCACACA TGCTGCTGAC TTGGAGACCG GCTGGTGCTG AAGGAGATCA CATGACTCAG 
CTGGAGTCTG ATAAACAGCT GGACTCCATT TCCCAGAAGT CAGTGGATAT TCAGACCCGC 
TCAGATGACC AGCAGTCTGT TAACAGTATG GAGAAGGCTT TGGCTGACAA CCGCAGATAC 
AGTCAGTTCA GTGTGGCTTC AGAAGCTCAG GAACAGAGGC TGTTTGCTGC GCTACTCATT 
AAGTGTGTGG TTCAGCTGGA GCTCATTCAG ACCATTGACA ACATCGTCTT CTTTCCTGCT 
ACCAGTAAGA AAGAAGATGC GGAAAACTTT GCGGCTGCAC AGAGAGATGC TTTAGATGCT 
GATATACATG TGGACACACA GGACCAGGGA ATGTATCGCT ATCTGACCTC AGAACAGCTC 
TTCAAACTGC TCGACTGTCT GCTGGAGTCT CATCGATTTG CAAAGGCCTT CAACTCCAAT 
AATGAGCAAA GAACTGCTCT GTGGAAAGCC GGTTTCAAAG GGAAGTCGAA GCCCAATCTG 
CTGAAACAGG AGACGAGCAG CCTGGCGTGT GGTCTGCGCA TCCTCTTCCG CATGTACACA 
GATCCAAGCC GACGGGATGC CTGGGAGGAG GTGCAGAGGA GACTGCTCAA CGTTTGCAGT 
GACGCAGTGG CTTATTTTCT GACCCTCACG TCTGAAAGTC ATCGCGAAGC CTGGACAAAC 
CTTCTGCTGC TCTTCCTGAC CAAAGTGCTG AAGATCAGTG ATGATAGATT CAAGGCTCAT 
GCGTCCCGCT ACTACCCTCT GCTCTGTGAA ATCATGCAGT TCGACCTGAT CCCGGAGCTG 
CGGGCCGTCC TGCGCAAGTT TTTCCTGCGC ATTGGACTGG TTTTCCAGAT CACCAACAGC 
CTGGAAACAA ATCAAACTGT CAGCAACGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_009297395.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295670.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X2, mRNA.

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CloneID	ODa49263
Clone ID Related Accession (Same CDS sequence)	XM_009297395.1
Accession Version	XM_009297395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA 
AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC 
AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT 
AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG 
CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC 
GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA 
CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC 
AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG 
ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC 
GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG 
TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG 
TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC 
ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC 
CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA 
CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA 
TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC 
AACCCCAATT CCCAGACAAG TCTTGGCCAA GAAAAGCCTT CAGAGCAGGA GAGCAATGAG 
TCGAAGCATC CAGAAACGAT CAACCGTTAC GGCAGCATTA ACTCTTTGGA CTCCACTGCA 
TCATCAGGCA TTGGGAGCTA CAGCACACAG ATGTCCGGAA CAGACAACCC GGAGCAGTTT 
GAGGTCCTCA AACAACAGAA GGAGATCATA GAGCAAGGCA TTGACCTGTT CAACAAAAAA 
CCAAAGAGGG GCATTCAGTA CCTGCAGGAG CAAGGCATGC TGGGAACAAC GCCTGAAGAT 
ATTGCCCAGT TTTTACATCA AGAGGAGAGA CTTGACTCTA TCCAGGTAGG AGAGTTTCTA 
GGAGACAACG ACCGCATCAA TAAAGAGGTC ATGTATGCCT ACGTCGACCA AATGGACTTC 
CAGGGCAAAG ACTTCGTGCC AGCGCTGCGC ATGTTCCTGG AGGGTTTCCG TCTGCCCGGC 
GAAGCCCAGA AAATTGATCG ACTGATGGAG AAATTTGCTG CTAGATATTT AGAATGCAAC 
CAAGGGCAAA CACTGTTCGC CAGCGCTGAC ACCGCATACG TTCTTGCGTA TTCAATCATC 
ATGTTGACTA CCGATCTGCA CAGTCCACAG GTGAAGAATA AAATGACGAA AGAGCAATAC 
ATCAAGATGA ATCGAGGTAT CAATGACAGT AAAGATTTAC CGGAGGAGTA TCTCTCAGCC 
ATTTATGATG AAATCGCAGG GAAGAAAATC TCCATGAAGG AGACAAAGGA GTTAACTCTC 
AAATCAAATA AGCAAAGTGT GGCGAGTGAG AAGCAGAGGA GGCTGCTGTA TAATGTTGAG 
ATGGAGCAGA TGGCAAAAAC AGCAAAGGCT CTGATGGAGG CCGTGAGCCA CGTTCAAGCC 
CCCTTCACCA GCGCCACACA TCTTGAGCAT GTCAGACCAA TGTTTAAGCT GGCCTGGACG 
CCCTTTCTGG CTGCTTTCAG TGTGGGTCTG CAGGACTGTG ATGACACTGA AGTGGCCTCT 
CTGTGTCTGG AGGGAATCCG CTGTGCCATC AGGATAGCAT GCATCTTCAG CATTCAGTTG 
GAGCGAGACG CATATGTACA GGCTCTGGCC CGTTTCACAC TCTTGACGGC CAGTTCTGGC 
ATCACTGAAA TGAAGCAGAA GAACATCGAC ACCATCAAGA CCCTCATCAC AGTGGCTCAC 
ACTGATGGAA ATTATCTAGG AAACTCCTGG CATGAGATCA TGAAGTGCAT TAGTCAGCTG 
GAGTTGGCCC AGCTCATCGG GACAGGAGTG AAGGCTCGGT ACATATCAGG AACTGTTCGG 
GGAAAGGAAG GCTTTATCAC CAGCACCAAG GAACAAACGT CTGATGAATA CCTGGGATTG 
GGAGGGAATG TTGATCGCAA ACAGATTGCA AGCATTCAGG AGTCTATCGG GGAGACCAGC 
TCGCAAAGTG TAGTTGTTGC TGTAGACCGG ATATTCACGG GCTCTACCAG ACTTGATGGA 
AATGCCATTG TGGACTTTGT GCGATGGCTG TGCGCTGTCT CGATGGATGA ACTGGCCTCC 
CCAACACACC CTCGAATGTT CAGTCTGCAG AAGATCGTGG AGATTTCATA CTACAATATG 
GGTCGTATCA GGTTGCAGTG GTCCAGGATT TGGGAGGTTA TAGGGGATCA TTTCAATAAG 
GTTGGCTGTA ATCCCAACGA AGATGTTGCT ATTTTTGCTG TGGATTCTCT GAGGCAGTTG 
TCCATGAAGT TTTTGGAAAA GGGAGAGTTG GCCAATTTCA GATTCCAGAA GGATTTCCTC 
AGGCCGTTCG AGCACATAAT GAAAAAGAAC AGATCTCCGA CCATTAGAGA CATGGTGGTT 
CGCTGTATTG CCCAGATGGT CAACTCTCAG GCTGGCAATA TTAGATCAGG GTGGAAGAAC 
ATTTTCTCTG TGTTTCATCT GGCTGCTTCT GATCAGGATG AGAGTATAGT TGAGCTGGCC 
TTCCAGACAA CTGGACACAT CGTCACGAAT GTATTTGAGA AGCATTTCCC AGCTACCATT 
GACTCCTTCC AGGACGCTGT CAAGTGTTTG TCTGAGTTCG CCTGCAACGC TTCTTTTCCA 
GACACCAGCA TGGAGGCTAT TCGTCTGATC CGCCACTGCG CCAAATATGT GTCAGACCGA 
CCCCAGGCGT TTAAAGACTA TACCAGTGAT GATATGAATG TTGCCCCGGA GGACCGTGTT 
TGGGTCAGAG GCTGGTTCCC CATCCTTTTT GAGCTGTCAT GCATCATCAA CAGATGCAAA 
CTGGACGTCC GGACCAGGGG TCTGACAGTG ATGTTTGAGG TGATGAAGAC GTACGGTCAT 
ACCTATGAGA AACACTGGTG GCAGGATCTG TTCCGCATCG TCTTCAGGAT CTTCGACAAC 
ATGAAGCTTC CTGAGCAACA GACCGAGAAA GCGGAGTGGA TGACCACCAC CTGCAATCAT 
GCACTTTATG CCATCTGTGA CGTTTTTACA CAGTACTTTG AGTCTCTCAG TGATGTTCTT 
TTAGATGATA TCTTATCTCA GCTCTACTGG TGTGTCCAGC AAGATAATGA GCAATTAGCC 
AGATCAGGCA CTAACTGCCT TGAAAACGTG GTCATCCTTA ATGGTGAGAA GTTCAATCCT 
GAGACTTGGG ACAAGACATG CAACTGCATG CTGGACATCT TCAAAACCAC CATCCCACAC 
ATGCTGCTGA CTTGGAGACC GGCTGGTGCT GAAGGAGATC ACATGACTCA GCTGGAGTCT 
GATAAACAGC TGGACTCCAT TTCCCAGAAG TCAGTGGATA TTCAGACCCG CTCAGATGAC 
CAGCAGTCTG TTAACAGTAT GGAGAAGGCT TTGGCTGACA ACCGCAGATA CAGTCAGTTC 
AGTGTGGCTT CAGAAGCTCA GGAACAGAGG CTGTTTGCTG CGCTACTCAT TAAGTGTGTG 
GTTCAGCTGG AGCTCATTCA GACCATTGAC AACATCGTCT TCTTTCCTGC TACCAGTAAG 
AAAGAAGATG CGGAAAACTT TGCGGCTGCA CAGAGAGATG CTTTAGATGC TGATATACAT 
GTGGACACAC AGGACCAGGG AATGTATCGC TATCTGACCT CAGAACAGCT CTTCAAACTG 
CTCGACTGTC TGCTGGAGTC TCATCGATTT GCAAAGGCCT TCAACTCCAA TAATGAGCAA 
AGAACTGCTC TGTGGAAAGC CGGTTTCAAA GGGAAGTCGA AGCCCAATCT GCTGAAACAG 
GAGACGAGCA GCCTGGCGTG TGGTCTGCGC ATCCTCTTCC GCATGTACAC AGATCCAAGC 
CGACGGGATG CCTGGGAGGA GGTGCAGAGG AGACTGCTCA ACGTTTGCAG TGACGCAGTG 
GCTTATTTTC TGACCCTCAC GTCTGAAAGT CATCGCGAAG CCTGGACAAA CCTTCTGCTG 
CTCTTCCTGA CCAAAGTGCT GAAGATCAGT GATGATAGAT TCAAGGCTCA TGCGTCCCGC 
TACTACCCTC TGCTCTGTGA AATCATGCAG TTCGACCTGA TCCCGGAGCT GCGGGCCGTC 
CTGCGCAAGT TTTTCCTGCG CATTGGACTG GTTTTCCAGA TCACCAACAG CCTGGAAACA 
AATCAAACTG TCAGCAACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295670.1
CDS	206..5809
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALTNHKISWSLASRKRDRQLQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATGNMYVTEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297395.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297395.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGGA 
AACATGTATG TTACAGAAAT GAAGGCAAGC CATGAGGGTG AGGCTGAGTC TGCGGAGGTC 
AGTCCAAACT CTCTGGAAGA GGAGGGAGGC TTGGGCAGCG ACAACGAAAA TGTTCACGCT 
AACGGCATCC CTGGCACTCC CATCTCCGCA AGCTTCACCC CATCCCTGCC CGACGACAGG 
CTGTCTGTCT CTTCTAATGA CACTCAGGAA TCGGTAGCCA CCTCTGGTCA AGCACCAGGC 
GCAAAGTTTT CTCACATTCT CCAGAAAGAT GCCTTCCTGG TGTTTCGTTC CCTCTGCAAA 
CTCTCCATGA AGCCTCTTTC GGATGGACCG CCTGATCCCA AATCCCATGA GCTGCGGTCC 
AAGGTCCTCT CTCTGCAGCT CCTCCTGTCC ATTCTGCAGA ACGCTGGACC CATCTTCAAG 
ACCAACGAGA TGTTCATCAA CGCCATCAAG CAGTACCTGT GCGTAGCGCT GTCTAAAAAC 
GGCGTCTCCT CCGTCCCCGA GGTGTTCGAG CTGTCTCTGT CCATCTTCCT CACCCTCCTG 
TCCAACTTCA AGACACATCT CAAGATGCAA ATCGAGGTGT TTTTCAAAGA GATTTTTCTG 
TATATTCTGG AAACTTCCAC AAGCTCATAT GACCACAAGT GGATGGTCAT TCAAACCCTC 
ACTAGAATAT GTGCTGATGC ACAGAGTGTA GTAGATATCT ATGTTAATTA TGATTGTGAC 
CTGAATGCTG CTAATATTTT CGAGAGGCTG GTGAATGACC TCTCAAAGAT TGCACAAGGA 
CGAGGAGGTC ATGAACTGGG TACAACCCCA CTTCAGGAGC TGACTTTAAG AAAGAAAGGA 
TTGGAGTGTC TGGTGTCCAT TCTCAAGTGT ATGGTGGAGT GGAGCAAAGA CCAATACGTC 
AACCCCAATT CCCAGACAAG TCTTGGCCAA GAAAAGCCTT CAGAGCAGGA GAGCAATGAG 
TCGAAGCATC CAGAAACGAT CAACCGTTAC GGCAGCATTA ACTCTTTGGA CTCCACTGCA 
TCATCAGGCA TTGGGAGCTA CAGCACACAG ATGTCCGGAA CAGACAACCC GGAGCAGTTT 
GAGGTCCTCA AACAACAGAA GGAGATCATA GAGCAAGGCA TTGACCTGTT CAACAAAAAA 
CCAAAGAGGG GCATTCAGTA CCTGCAGGAG CAAGGCATGC TGGGAACAAC GCCTGAAGAT 
ATTGCCCAGT TTTTACATCA AGAGGAGAGA CTTGACTCTA TCCAGGTAGG AGAGTTTCTA 
GGAGACAACG ACCGCATCAA TAAAGAGGTC ATGTATGCCT ACGTCGACCA AATGGACTTC 
CAGGGCAAAG ACTTCGTGCC AGCGCTGCGC ATGTTCCTGG AGGGTTTCCG TCTGCCCGGC 
GAAGCCCAGA AAATTGATCG ACTGATGGAG AAATTTGCTG CTAGATATTT AGAATGCAAC 
CAAGGGCAAA CACTGTTCGC CAGCGCTGAC ACCGCATACG TTCTTGCGTA TTCAATCATC 
ATGTTGACTA CCGATCTGCA CAGTCCACAG GTGAAGAATA AAATGACGAA AGAGCAATAC 
ATCAAGATGA ATCGAGGTAT CAATGACAGT AAAGATTTAC CGGAGGAGTA TCTCTCAGCC 
ATTTATGATG AAATCGCAGG GAAGAAAATC TCCATGAAGG AGACAAAGGA GTTAACTCTC 
AAATCAAATA AGCAAAGTGT GGCGAGTGAG AAGCAGAGGA GGCTGCTGTA TAATGTTGAG 
ATGGAGCAGA TGGCAAAAAC AGCAAAGGCT CTGATGGAGG CCGTGAGCCA CGTTCAAGCC 
CCCTTCACCA GCGCCACACA TCTTGAGCAT GTCAGACCAA TGTTTAAGCT GGCCTGGACG 
CCCTTTCTGG CTGCTTTCAG TGTGGGTCTG CAGGACTGTG ATGACACTGA AGTGGCCTCT 
CTGTGTCTGG AGGGAATCCG CTGTGCCATC AGGATAGCAT GCATCTTCAG CATTCAGTTG 
GAGCGAGACG CATATGTACA GGCTCTGGCC CGTTTCACAC TCTTGACGGC CAGTTCTGGC 
ATCACTGAAA TGAAGCAGAA GAACATCGAC ACCATCAAGA CCCTCATCAC AGTGGCTCAC 
ACTGATGGAA ATTATCTAGG AAACTCCTGG CATGAGATCA TGAAGTGCAT TAGTCAGCTG 
GAGTTGGCCC AGCTCATCGG GACAGGAGTG AAGGCTCGGT ACATATCAGG AACTGTTCGG 
GGAAAGGAAG GCTTTATCAC CAGCACCAAG GAACAAACGT CTGATGAATA CCTGGGATTG 
GGAGGGAATG TTGATCGCAA ACAGATTGCA AGCATTCAGG AGTCTATCGG GGAGACCAGC 
TCGCAAAGTG TAGTTGTTGC TGTAGACCGG ATATTCACGG GCTCTACCAG ACTTGATGGA 
AATGCCATTG TGGACTTTGT GCGATGGCTG TGCGCTGTCT CGATGGATGA ACTGGCCTCC 
CCAACACACC CTCGAATGTT CAGTCTGCAG AAGATCGTGG AGATTTCATA CTACAATATG 
GGTCGTATCA GGTTGCAGTG GTCCAGGATT TGGGAGGTTA TAGGGGATCA TTTCAATAAG 
GTTGGCTGTA ATCCCAACGA AGATGTTGCT ATTTTTGCTG TGGATTCTCT GAGGCAGTTG 
TCCATGAAGT TTTTGGAAAA GGGAGAGTTG GCCAATTTCA GATTCCAGAA GGATTTCCTC 
AGGCCGTTCG AGCACATAAT GAAAAAGAAC AGATCTCCGA CCATTAGAGA CATGGTGGTT 
CGCTGTATTG CCCAGATGGT CAACTCTCAG GCTGGCAATA TTAGATCAGG GTGGAAGAAC 
ATTTTCTCTG TGTTTCATCT GGCTGCTTCT GATCAGGATG AGAGTATAGT TGAGCTGGCC 
TTCCAGACAA CTGGACACAT CGTCACGAAT GTATTTGAGA AGCATTTCCC AGCTACCATT 
GACTCCTTCC AGGACGCTGT CAAGTGTTTG TCTGAGTTCG CCTGCAACGC TTCTTTTCCA 
GACACCAGCA TGGAGGCTAT TCGTCTGATC CGCCACTGCG CCAAATATGT GTCAGACCGA 
CCCCAGGCGT TTAAAGACTA TACCAGTGAT GATATGAATG TTGCCCCGGA GGACCGTGTT 
TGGGTCAGAG GCTGGTTCCC CATCCTTTTT GAGCTGTCAT GCATCATCAA CAGATGCAAA 
CTGGACGTCC GGACCAGGGG TCTGACAGTG ATGTTTGAGG TGATGAAGAC GTACGGTCAT 
ACCTATGAGA AACACTGGTG GCAGGATCTG TTCCGCATCG TCTTCAGGAT CTTCGACAAC 
ATGAAGCTTC CTGAGCAACA GACCGAGAAA GCGGAGTGGA TGACCACCAC CTGCAATCAT 
GCACTTTATG CCATCTGTGA CGTTTTTACA CAGTACTTTG AGTCTCTCAG TGATGTTCTT 
TTAGATGATA TCTTATCTCA GCTCTACTGG TGTGTCCAGC AAGATAATGA GCAATTAGCC 
AGATCAGGCA CTAACTGCCT TGAAAACGTG GTCATCCTTA ATGGTGAGAA GTTCAATCCT 
GAGACTTGGG ACAAGACATG CAACTGCATG CTGGACATCT TCAAAACCAC CATCCCACAC 
ATGCTGCTGA CTTGGAGACC GGCTGGTGCT GAAGGAGATC ACATGACTCA GCTGGAGTCT 
GATAAACAGC TGGACTCCAT TTCCCAGAAG TCAGTGGATA TTCAGACCCG CTCAGATGAC 
CAGCAGTCTG TTAACAGTAT GGAGAAGGCT TTGGCTGACA ACCGCAGATA CAGTCAGTTC 
AGTGTGGCTT CAGAAGCTCA GGAACAGAGG CTGTTTGCTG CGCTACTCAT TAAGTGTGTG 
GTTCAGCTGG AGCTCATTCA GACCATTGAC AACATCGTCT TCTTTCCTGC TACCAGTAAG 
AAAGAAGATG CGGAAAACTT TGCGGCTGCA CAGAGAGATG CTTTAGATGC TGATATACAT 
GTGGACACAC AGGACCAGGG AATGTATCGC TATCTGACCT CAGAACAGCT CTTCAAACTG 
CTCGACTGTC TGCTGGAGTC TCATCGATTT GCAAAGGCCT TCAACTCCAA TAATGAGCAA 
AGAACTGCTC TGTGGAAAGC CGGTTTCAAA GGGAAGTCGA AGCCCAATCT GCTGAAACAG 
GAGACGAGCA GCCTGGCGTG TGGTCTGCGC ATCCTCTTCC GCATGTACAC AGATCCAAGC 
CGACGGGATG CCTGGGAGGA GGTGCAGAGG AGACTGCTCA ACGTTTGCAG TGACGCAGTG 
GCTTATTTTC TGACCCTCAC GTCTGAAAGT CATCGCGAAG CCTGGACAAA CCTTCTGCTG 
CTCTTCCTGA CCAAAGTGCT GAAGATCAGT GATGATAGAT TCAAGGCTCA TGCGTCCCGC 
TACTACCCTC TGCTCTGTGA AATCATGCAG TTCGACCTGA TCCCGGAGCT GCGGGCCGTC 
CTGCGCAAGT TTTTCCTGCG CATTGGACTG GTTTTCCAGA TCACCAACAG CCTGGAAACA 
AATCAAACTG TCAGCAACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_009297396.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295671.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X3, mRNA.

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CloneID	ODa49264
Clone ID Related Accession (Same CDS sequence)	XM_009297396.1
Accession Version	XM_009297396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGAA 
ATGAAGGCAA GCCATGAGGG TGAGGCTGAG TCTGCGGAGG TCAGTCCAAA CTCTCTGGAA 
GAGGAGGGAG GCTTGGGCAG CGACAACGAA AATGTTCACG CTAACGGCAT CCCTGGCACT 
CCCATCTCCG CAAGCTTCAC CCCATCCCTG CCCGACGACA GGCTGTCTGT CTCTTCTAAT 
GACACTCAGG AATCGGTAGC CACCTCTGGT CAAGCACCAG GCGCAAAGTT TTCTCACATT 
CTCCAGAAAG ATGCCTTCCT GGTGTTTCGT TCCCTCTGCA AACTCTCCAT GAAGCCTCTT 
TCGGATGGAC CGCCTGATCC CAAATCCCAT GAGCTGCGGT CCAAGGTCCT CTCTCTGCAG 
CTCCTCCTGT CCATTCTGCA GAACGCTGGA CCCATCTTCA AGACCAACGA GATGTTCATC 
AACGCCATCA AGCAGTACCT GTGCGTAGCG CTGTCTAAAA ACGGCGTCTC CTCCGTCCCC 
GAGGTGTTCG AGCTGTCTCT GTCCATCTTC CTCACCCTCC TGTCCAACTT CAAGACACAT 
CTCAAGATGC AAATCGAGGT GTTTTTCAAA GAGATTTTTC TGTATATTCT GGAAACTTCC 
ACAAGCTCAT ATGACCACAA GTGGATGGTC ATTCAAACCC TCACTAGAAT ATGTGCTGAT 
GCACAGAGTG TAGTAGATAT CTATGTTAAT TATGATTGTG ACCTGAATGC TGCTAATATT 
TTCGAGAGGC TGGTGAATGA CCTCTCAAAG ATTGCACAAG GACGAGGAGG TCATGAACTG 
GGTACAACCC CACTTCAGGA GCTGACTTTA AGAAAGAAAG GATTGGAGTG TCTGGTGTCC 
ATTCTCAAGT GTATGGTGGA GTGGAGCAAA GACCAATACG TCAACCCCAA TTCCCAGACA 
AGTCTTGGGA GAGCAGGCCA AGAAAAGCCT TCAGAGCAGG AGAGCAATGA GTCGAAGCAT 
CCAGAAACGA TCAACCGTTA CGGCAGCATT AACTCTTTGG ACTCCACTGC ATCATCAGGC 
ATTGGGAGCT ACAGCACACA GATGTCCGGA ACAGACAACC CGGAGCAGTT TGAGGTCCTC 
AAACAACAGA AGGAGATCAT AGAGCAAGGC ATTGACCTGT TCAACAAAAA ACCAAAGAGG 
GGCATTCAGT ACCTGCAGGA GCAAGGCATG CTGGGAACAA CGCCTGAAGA TATTGCCCAG 
TTTTTACATC AAGAGGAGAG ACTTGACTCT ATCCAGGTAG GAGAGTTTCT AGGAGACAAC 
GACCGCATCA ATAAAGAGGT CATGTATGCC TACGTCGACC AAATGGACTT CCAGGGCAAA 
GACTTCGTGC CAGCGCTGCG CATGTTCCTG GAGGGTTTCC GTCTGCCCGG CGAAGCCCAG 
AAAATTGATC GACTGATGGA GAAATTTGCT GCTAGATATT TAGAATGCAA CCAAGGGCAA 
ACACTGTTCG CCAGCGCTGA CACCGCATAC GTTCTTGCGT ATTCAATCAT CATGTTGACT 
ACCGATCTGC ACAGTCCACA GGTGAAGAAT AAAATGACGA AAGAGCAATA CATCAAGATG 
AATCGAGGTA TCAATGACAG TAAAGATTTA CCGGAGGAGT ATCTCTCAGC CATTTATGAT 
GAAATCGCAG GGAAGAAAAT CTCCATGAAG GAGACAAAGG AGTTAACTCT CAAATCAAAT 
AAGCAAAGTG TGGCGAGTGA GAAGCAGAGG AGGCTGCTGT ATAATGTTGA GATGGAGCAG 
ATGGCAAAAA CAGCAAAGGC TCTGATGGAG GCCGTGAGCC ACGTTCAAGC CCCCTTCACC 
AGCGCCACAC ATCTTGAGCA TGTCAGACCA ATGTTTAAGC TGGCCTGGAC GCCCTTTCTG 
GCTGCTTTCA GTGTGGGTCT GCAGGACTGT GATGACACTG AAGTGGCCTC TCTGTGTCTG 
GAGGGAATCC GCTGTGCCAT CAGGATAGCA TGCATCTTCA GCATTCAGTT GGAGCGAGAC 
GCATATGTAC AGGCTCTGGC CCGTTTCACA CTCTTGACGG CCAGTTCTGG CATCACTGAA 
ATGAAGCAGA AGAACATCGA CACCATCAAG ACCCTCATCA CAGTGGCTCA CACTGATGGA 
AATTATCTAG GAAACTCCTG GCATGAGATC ATGAAGTGCA TTAGTCAGCT GGAGTTGGCC 
CAGCTCATCG GGACAGGAGT GAAGGCTCGG TACATATCAG GAACTGTTCG GGGAAAGGAA 
GGCTTTATCA CCAGCACCAA GGAACAAACG TCTGATGAAT ACCTGGGATT GGGAGGGAAT 
GTTGATCGCA AACAGATTGC AAGCATTCAG GAGTCTATCG GGGAGACCAG CTCGCAAAGT 
GTAGTTGTTG CTGTAGACCG GATATTCACG GGCTCTACCA GACTTGATGG AAATGCCATT 
GTGGACTTTG TGCGATGGCT GTGCGCTGTC TCGATGGATG AACTGGCCTC CCCAACACAC 
CCTCGAATGT TCAGTCTGCA GAAGATCGTG GAGATTTCAT ACTACAATAT GGGTCGTATC 
AGGTTGCAGT GGTCCAGGAT TTGGGAGGTT ATAGGGGATC ATTTCAATAA GGTTGGCTGT 
AATCCCAACG AAGATGTTGC TATTTTTGCT GTGGATTCTC TGAGGCAGTT GTCCATGAAG 
TTTTTGGAAA AGGGAGAGTT GGCCAATTTC AGATTCCAGA AGGATTTCCT CAGGCCGTTC 
GAGCACATAA TGAAAAAGAA CAGATCTCCG ACCATTAGAG ACATGGTGGT TCGCTGTATT 
GCCCAGATGG TCAACTCTCA GGCTGGCAAT ATTAGATCAG GGTGGAAGAA CATTTTCTCT 
GTGTTTCATC TGGCTGCTTC TGATCAGGAT GAGAGTATAG TTGAGCTGGC CTTCCAGACA 
ACTGGACACA TCGTCACGAA TGTATTTGAG AAGCATTTCC CAGCTACCAT TGACTCCTTC 
CAGGACGCTG TCAAGTGTTT GTCTGAGTTC GCCTGCAACG CTTCTTTTCC AGACACCAGC 
ATGGAGGCTA TTCGTCTGAT CCGCCACTGC GCCAAATATG TGTCAGACCG ACCCCAGGCG 
TTTAAAGACT ATACCAGTGA TGATATGAAT GTTGCCCCGG AGGACCGTGT TTGGGTCAGA 
GGCTGGTTCC CCATCCTTTT TGAGCTGTCA TGCATCATCA ACAGATGCAA ACTGGACGTC 
CGGACCAGGG GTCTGACAGT GATGTTTGAG GTGATGAAGA CGTACGGTCA TACCTATGAG 
AAACACTGGT GGCAGGATCT GTTCCGCATC GTCTTCAGGA TCTTCGACAA CATGAAGCTT 
CCTGAGCAAC AGACCGAGAA AGCGGAGTGG ATGACCACCA CCTGCAATCA TGCACTTTAT 
GCCATCTGTG ACGTTTTTAC ACAGTACTTT GAGTCTCTCA GTGATGTTCT TTTAGATGAT 
ATCTTATCTC AGCTCTACTG GTGTGTCCAG CAAGATAATG AGCAATTAGC CAGATCAGGC 
ACTAACTGCC TTGAAAACGT GGTCATCCTT AATGGTGAGA AGTTCAATCC TGAGACTTGG 
GACAAGACAT GCAACTGCAT GCTGGACATC TTCAAAACCA CCATCCCACA CATGCTGCTG 
ACTTGGAGAC CGGCTGGTGC TGAAGGAGAT CACATGACTC AGCTGGAGTC TGATAAACAG 
CTGGACTCCA TTTCCCAGAA GTCAGTGGAT ATTCAGACCC GCTCAGATGA CCAGCAGTCT 
GTTAACAGTA TGGAGAAGGC TTTGGCTGAC AACCGCAGAT ACAGTCAGTT CAGTGTGGCT 
TCAGAAGCTC AGGAACAGAG GCTGTTTGCT GCGCTACTCA TTAAGTGTGT GGTTCAGCTG 
GAGCTCATTC AGACCATTGA CAACATCGTC TTCTTTCCTG CTACCAGTAA GAAAGAAGAT 
GCGGAAAACT TTGCGGCTGC ACAGAGAGAT GCTTTAGATG CTGATATACA TGTGGACACA 
CAGGACCAGG GAATGTATCG CTATCTGACC TCAGAACAGC TCTTCAAACT GCTCGACTGT 
CTGCTGGAGT CTCATCGATT TGCAAAGGCC TTCAACTCCA ATAATGAGCA AAGAACTGCT 
CTGTGGAAAG CCGGTTTCAA AGGGAAGTCG AAGCCCAATC TGCTGAAACA GGAGACGAGC 
AGCCTGGCGT GTGGTCTGCG CATCCTCTTC CGCATGTACA CAGATCCAAG CCGACGGGAT 
GCCTGGGAGG AGGTGCAGAG GAGACTGCTC AACGTTTGCA GTGACGCAGT GGCTTATTTT 
CTGACCCTCA CGTCTGAAAG TCATCGCGAA GCCTGGACAA ACCTTCTGCT GCTCTTCCTG 
ACCAAAGTGC TGAAGATCAG TGATGATAGA TTCAAGGCTC ATGCGTCCCG CTACTACCCT 
CTGCTCTGTG AAATCATGCA GTTCGACCTG ATCCCGGAGC TGCGGGCCGT CCTGCGCAAG 
TTTTTCCTGC GCATTGGACT GGTTTTCCAG ATCACCAACA GCCTGGAAAC AAATCAAACT 
GTCAGCAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295671.1
CDS	206..5800
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALTNHKISWSLASRKRDRQLQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGRAGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297396.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297396.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TTACAAATCA CAAGATCAGT 
TGGTCTTTGG CATCAAGAAA GCGAGATCGT CAGCTGCAGG AAGCCAAGCA GATGGAGAGA 
GAGCGCCACA GACAGCATTC TCCTGTCAGT CAGCAAGAGC CTGAATCTCC TGGACAGAGG 
CACACCGAGA CCCCTGGCAA AACCCTGCCA GCTGAGGGCA ACGGTGGACC TGGACATCAG 
GGTGGTAAAG TGGAGCAGGG TTCCCCACCG TATGAGAGTC CCGAGCCAGA GAATGGAAGT 
GACTTCTGTC CTGCGGAGAA TGAGCAGACC GAGGCTGACC AGGCCACCGC TGCAGCACAA 
AGTGCAGCAG AGCATGCCAC TGAAGCCAGC GAGGAGGATG AAAGGCAGAA CTATGAAGAG 
AAGGCGCAGG AGATTGTGGA GAGCGTTTTG CTAGAAGTGG TGAACACAGT GGCTACAGAA 
ATGAAGGCAA GCCATGAGGG TGAGGCTGAG TCTGCGGAGG TCAGTCCAAA CTCTCTGGAA 
GAGGAGGGAG GCTTGGGCAG CGACAACGAA AATGTTCACG CTAACGGCAT CCCTGGCACT 
CCCATCTCCG CAAGCTTCAC CCCATCCCTG CCCGACGACA GGCTGTCTGT CTCTTCTAAT 
GACACTCAGG AATCGGTAGC CACCTCTGGT CAAGCACCAG GCGCAAAGTT TTCTCACATT 
CTCCAGAAAG ATGCCTTCCT GGTGTTTCGT TCCCTCTGCA AACTCTCCAT GAAGCCTCTT 
TCGGATGGAC CGCCTGATCC CAAATCCCAT GAGCTGCGGT CCAAGGTCCT CTCTCTGCAG 
CTCCTCCTGT CCATTCTGCA GAACGCTGGA CCCATCTTCA AGACCAACGA GATGTTCATC 
AACGCCATCA AGCAGTACCT GTGCGTAGCG CTGTCTAAAA ACGGCGTCTC CTCCGTCCCC 
GAGGTGTTCG AGCTGTCTCT GTCCATCTTC CTCACCCTCC TGTCCAACTT CAAGACACAT 
CTCAAGATGC AAATCGAGGT GTTTTTCAAA GAGATTTTTC TGTATATTCT GGAAACTTCC 
ACAAGCTCAT ATGACCACAA GTGGATGGTC ATTCAAACCC TCACTAGAAT ATGTGCTGAT 
GCACAGAGTG TAGTAGATAT CTATGTTAAT TATGATTGTG ACCTGAATGC TGCTAATATT 
TTCGAGAGGC TGGTGAATGA CCTCTCAAAG ATTGCACAAG GACGAGGAGG TCATGAACTG 
GGTACAACCC CACTTCAGGA GCTGACTTTA AGAAAGAAAG GATTGGAGTG TCTGGTGTCC 
ATTCTCAAGT GTATGGTGGA GTGGAGCAAA GACCAATACG TCAACCCCAA TTCCCAGACA 
AGTCTTGGGA GAGCAGGCCA AGAAAAGCCT TCAGAGCAGG AGAGCAATGA GTCGAAGCAT 
CCAGAAACGA TCAACCGTTA CGGCAGCATT AACTCTTTGG ACTCCACTGC ATCATCAGGC 
ATTGGGAGCT ACAGCACACA GATGTCCGGA ACAGACAACC CGGAGCAGTT TGAGGTCCTC 
AAACAACAGA AGGAGATCAT AGAGCAAGGC ATTGACCTGT TCAACAAAAA ACCAAAGAGG 
GGCATTCAGT ACCTGCAGGA GCAAGGCATG CTGGGAACAA CGCCTGAAGA TATTGCCCAG 
TTTTTACATC AAGAGGAGAG ACTTGACTCT ATCCAGGTAG GAGAGTTTCT AGGAGACAAC 
GACCGCATCA ATAAAGAGGT CATGTATGCC TACGTCGACC AAATGGACTT CCAGGGCAAA 
GACTTCGTGC CAGCGCTGCG CATGTTCCTG GAGGGTTTCC GTCTGCCCGG CGAAGCCCAG 
AAAATTGATC GACTGATGGA GAAATTTGCT GCTAGATATT TAGAATGCAA CCAAGGGCAA 
ACACTGTTCG CCAGCGCTGA CACCGCATAC GTTCTTGCGT ATTCAATCAT CATGTTGACT 
ACCGATCTGC ACAGTCCACA GGTGAAGAAT AAAATGACGA AAGAGCAATA CATCAAGATG 
AATCGAGGTA TCAATGACAG TAAAGATTTA CCGGAGGAGT ATCTCTCAGC CATTTATGAT 
GAAATCGCAG GGAAGAAAAT CTCCATGAAG GAGACAAAGG AGTTAACTCT CAAATCAAAT 
AAGCAAAGTG TGGCGAGTGA GAAGCAGAGG AGGCTGCTGT ATAATGTTGA GATGGAGCAG 
ATGGCAAAAA CAGCAAAGGC TCTGATGGAG GCCGTGAGCC ACGTTCAAGC CCCCTTCACC 
AGCGCCACAC ATCTTGAGCA TGTCAGACCA ATGTTTAAGC TGGCCTGGAC GCCCTTTCTG 
GCTGCTTTCA GTGTGGGTCT GCAGGACTGT GATGACACTG AAGTGGCCTC TCTGTGTCTG 
GAGGGAATCC GCTGTGCCAT CAGGATAGCA TGCATCTTCA GCATTCAGTT GGAGCGAGAC 
GCATATGTAC AGGCTCTGGC CCGTTTCACA CTCTTGACGG CCAGTTCTGG CATCACTGAA 
ATGAAGCAGA AGAACATCGA CACCATCAAG ACCCTCATCA CAGTGGCTCA CACTGATGGA 
AATTATCTAG GAAACTCCTG GCATGAGATC ATGAAGTGCA TTAGTCAGCT GGAGTTGGCC 
CAGCTCATCG GGACAGGAGT GAAGGCTCGG TACATATCAG GAACTGTTCG GGGAAAGGAA 
GGCTTTATCA CCAGCACCAA GGAACAAACG TCTGATGAAT ACCTGGGATT GGGAGGGAAT 
GTTGATCGCA AACAGATTGC AAGCATTCAG GAGTCTATCG GGGAGACCAG CTCGCAAAGT 
GTAGTTGTTG CTGTAGACCG GATATTCACG GGCTCTACCA GACTTGATGG AAATGCCATT 
GTGGACTTTG TGCGATGGCT GTGCGCTGTC TCGATGGATG AACTGGCCTC CCCAACACAC 
CCTCGAATGT TCAGTCTGCA GAAGATCGTG GAGATTTCAT ACTACAATAT GGGTCGTATC 
AGGTTGCAGT GGTCCAGGAT TTGGGAGGTT ATAGGGGATC ATTTCAATAA GGTTGGCTGT 
AATCCCAACG AAGATGTTGC TATTTTTGCT GTGGATTCTC TGAGGCAGTT GTCCATGAAG 
TTTTTGGAAA AGGGAGAGTT GGCCAATTTC AGATTCCAGA AGGATTTCCT CAGGCCGTTC 
GAGCACATAA TGAAAAAGAA CAGATCTCCG ACCATTAGAG ACATGGTGGT TCGCTGTATT 
GCCCAGATGG TCAACTCTCA GGCTGGCAAT ATTAGATCAG GGTGGAAGAA CATTTTCTCT 
GTGTTTCATC TGGCTGCTTC TGATCAGGAT GAGAGTATAG TTGAGCTGGC CTTCCAGACA 
ACTGGACACA TCGTCACGAA TGTATTTGAG AAGCATTTCC CAGCTACCAT TGACTCCTTC 
CAGGACGCTG TCAAGTGTTT GTCTGAGTTC GCCTGCAACG CTTCTTTTCC AGACACCAGC 
ATGGAGGCTA TTCGTCTGAT CCGCCACTGC GCCAAATATG TGTCAGACCG ACCCCAGGCG 
TTTAAAGACT ATACCAGTGA TGATATGAAT GTTGCCCCGG AGGACCGTGT TTGGGTCAGA 
GGCTGGTTCC CCATCCTTTT TGAGCTGTCA TGCATCATCA ACAGATGCAA ACTGGACGTC 
CGGACCAGGG GTCTGACAGT GATGTTTGAG GTGATGAAGA CGTACGGTCA TACCTATGAG 
AAACACTGGT GGCAGGATCT GTTCCGCATC GTCTTCAGGA TCTTCGACAA CATGAAGCTT 
CCTGAGCAAC AGACCGAGAA AGCGGAGTGG ATGACCACCA CCTGCAATCA TGCACTTTAT 
GCCATCTGTG ACGTTTTTAC ACAGTACTTT GAGTCTCTCA GTGATGTTCT TTTAGATGAT 
ATCTTATCTC AGCTCTACTG GTGTGTCCAG CAAGATAATG AGCAATTAGC CAGATCAGGC 
ACTAACTGCC TTGAAAACGT GGTCATCCTT AATGGTGAGA AGTTCAATCC TGAGACTTGG 
GACAAGACAT GCAACTGCAT GCTGGACATC TTCAAAACCA CCATCCCACA CATGCTGCTG 
ACTTGGAGAC CGGCTGGTGC TGAAGGAGAT CACATGACTC AGCTGGAGTC TGATAAACAG 
CTGGACTCCA TTTCCCAGAA GTCAGTGGAT ATTCAGACCC GCTCAGATGA CCAGCAGTCT 
GTTAACAGTA TGGAGAAGGC TTTGGCTGAC AACCGCAGAT ACAGTCAGTT CAGTGTGGCT 
TCAGAAGCTC AGGAACAGAG GCTGTTTGCT GCGCTACTCA TTAAGTGTGT GGTTCAGCTG 
GAGCTCATTC AGACCATTGA CAACATCGTC TTCTTTCCTG CTACCAGTAA GAAAGAAGAT 
GCGGAAAACT TTGCGGCTGC ACAGAGAGAT GCTTTAGATG CTGATATACA TGTGGACACA 
CAGGACCAGG GAATGTATCG CTATCTGACC TCAGAACAGC TCTTCAAACT GCTCGACTGT 
CTGCTGGAGT CTCATCGATT TGCAAAGGCC TTCAACTCCA ATAATGAGCA AAGAACTGCT 
CTGTGGAAAG CCGGTTTCAA AGGGAAGTCG AAGCCCAATC TGCTGAAACA GGAGACGAGC 
AGCCTGGCGT GTGGTCTGCG CATCCTCTTC CGCATGTACA CAGATCCAAG CCGACGGGAT 
GCCTGGGAGG AGGTGCAGAG GAGACTGCTC AACGTTTGCA GTGACGCAGT GGCTTATTTT 
CTGACCCTCA CGTCTGAAAG TCATCGCGAA GCCTGGACAA ACCTTCTGCT GCTCTTCCTG 
ACCAAAGTGC TGAAGATCAG TGATGATAGA TTCAAGGCTC ATGCGTCCCG CTACTACCCT 
CTGCTCTGTG AAATCATGCA GTTCGACCTG ATCCCGGAGC TGCGGGCCGT CCTGCGCAAG 
TTTTTCCTGC GCATTGGACT GGTTTTCCAG ATCACCAACA GCCTGGAAAC AAATCAAACT 
GTCAGCAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_009297397.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295672.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa49265
Clone ID Related Accession (Same CDS sequence)	XM_009297397.1
Accession Version	XM_009297397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG 
GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT 
CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC 
GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA 
CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC 
TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG 
CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC 
TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT 
AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC 
CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT 
TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA 
ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT 
TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA 
CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG 
AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA 
TACGTCAACC CCAATTCCCA GACAAGTCTT GGGAGAGCAG GCCAAGAAAA GCCTTCAGAG 
CAGGAGAGCA ATGAGTCGAA GCATCCAGAA ACGATCAACC GTTACGGCAG CATTAACTCT 
TTGGACTCCA CTGCATCATC AGGCATTGGG AGCTACAGCA CACAGATGTC CGGAACAGAC 
AACCCGGAGC AGTTTGAGGT CCTCAAACAA CAGAAGGAGA TCATAGAGCA AGGCATTGAC 
CTGTTCAACA AAAAACCAAA GAGGGGCATT CAGTACCTGC AGGAGCAAGG CATGCTGGGA 
ACAACGCCTG AAGATATTGC CCAGTTTTTA CATCAAGAGG AGAGACTTGA CTCTATCCAG 
GTAGGAGAGT TTCTAGGAGA CAACGACCGC ATCAATAAAG AGGTCATGTA TGCCTACGTC 
GACCAAATGG ACTTCCAGGG CAAAGACTTC GTGCCAGCGC TGCGCATGTT CCTGGAGGGT 
TTCCGTCTGC CCGGCGAAGC CCAGAAAATT GATCGACTGA TGGAGAAATT TGCTGCTAGA 
TATTTAGAAT GCAACCAAGG GCAAACACTG TTCGCCAGCG CTGACACCGC ATACGTTCTT 
GCGTATTCAA TCATCATGTT GACTACCGAT CTGCACAGTC CACAGGTGAA GAATAAAATG 
ACGAAAGAGC AATACATCAA GATGAATCGA GGTATCAATG ACAGTAAAGA TTTACCGGAG 
GAGTATCTCT CAGCCATTTA TGATGAAATC GCAGGGAAGA AAATCTCCAT GAAGGAGACA 
AAGGAGTTAA CTCTCAAATC AAATAAGCAA AGTGTGGCGA GTGAGAAGCA GAGGAGGCTG 
CTGTATAATG TTGAGATGGA GCAGATGGCA AAAACAGCAA AGGCTCTGAT GGAGGCCGTG 
AGCCACGTTC AAGCCCCCTT CACCAGCGCC ACACATCTTG AGCATGTCAG ACCAATGTTT 
AAGCTGGCCT GGACGCCCTT TCTGGCTGCT TTCAGTGTGG GTCTGCAGGA CTGTGATGAC 
ACTGAAGTGG CCTCTCTGTG TCTGGAGGGA ATCCGCTGTG CCATCAGGAT AGCATGCATC 
TTCAGCATTC AGTTGGAGCG AGACGCATAT GTACAGGCTC TGGCCCGTTT CACACTCTTG 
ACGGCCAGTT CTGGCATCAC TGAAATGAAG CAGAAGAACA TCGACACCAT CAAGACCCTC 
ATCACAGTGG CTCACACTGA TGGAAATTAT CTAGGAAACT CCTGGCATGA GATCATGAAG 
TGCATTAGTC AGCTGGAGTT GGCCCAGCTC ATCGGGACAG GAGTGAAGGC TCGGTACATA 
TCAGGAACTG TTCGGGGAAA GGAAGGCTTT ATCACCAGCA CCAAGGAACA AACGTCTGAT 
GAATACCTGG GATTGGGAGG GAATGTTGAT CGCAAACAGA TTGCAAGCAT TCAGGAGTCT 
ATCGGGGAGA CCAGCTCGCA AAGTGTAGTT GTTGCTGTAG ACCGGATATT CACGGGCTCT 
ACCAGACTTG ATGGAAATGC CATTGTGGAC TTTGTGCGAT GGCTGTGCGC TGTCTCGATG 
GATGAACTGG CCTCCCCAAC ACACCCTCGA ATGTTCAGTC TGCAGAAGAT CGTGGAGATT 
TCATACTACA ATATGGGTCG TATCAGGTTG CAGTGGTCCA GGATTTGGGA GGTTATAGGG 
GATCATTTCA ATAAGGTTGG CTGTAATCCC AACGAAGATG TTGCTATTTT TGCTGTGGAT 
TCTCTGAGGC AGTTGTCCAT GAAGTTTTTG GAAAAGGGAG AGTTGGCCAA TTTCAGATTC 
CAGAAGGATT TCCTCAGGCC GTTCGAGCAC ATAATGAAAA AGAACAGATC TCCGACCATT 
AGAGACATGG TGGTTCGCTG TATTGCCCAG ATGGTCAACT CTCAGGCTGG CAATATTAGA 
TCAGGGTGGA AGAACATTTT CTCTGTGTTT CATCTGGCTG CTTCTGATCA GGATGAGAGT 
ATAGTTGAGC TGGCCTTCCA GACAACTGGA CACATCGTCA CGAATGTATT TGAGAAGCAT 
TTCCCAGCTA CCATTGACTC CTTCCAGGAC GCTGTCAAGT GTTTGTCTGA GTTCGCCTGC 
AACGCTTCTT TTCCAGACAC CAGCATGGAG GCTATTCGTC TGATCCGCCA CTGCGCCAAA 
TATGTGTCAG ACCGACCCCA GGCGTTTAAA GACTATACCA GTGATGATAT GAATGTTGCC 
CCGGAGGACC GTGTTTGGGT CAGAGGCTGG TTCCCCATCC TTTTTGAGCT GTCATGCATC 
ATCAACAGAT GCAAACTGGA CGTCCGGACC AGGGGTCTGA CAGTGATGTT TGAGGTGATG 
AAGACGTACG GTCATACCTA TGAGAAACAC TGGTGGCAGG ATCTGTTCCG CATCGTCTTC 
AGGATCTTCG ACAACATGAA GCTTCCTGAG CAACAGACCG AGAAAGCGGA GTGGATGACC 
ACCACCTGCA ATCATGCACT TTATGCCATC TGTGACGTTT TTACACAGTA CTTTGAGTCT 
CTCAGTGATG TTCTTTTAGA TGATATCTTA TCTCAGCTCT ACTGGTGTGT CCAGCAAGAT 
AATGAGCAAT TAGCCAGATC AGGCACTAAC TGCCTTGAAA ACGTGGTCAT CCTTAATGGT 
GAGAAGTTCA ATCCTGAGAC TTGGGACAAG ACATGCAACT GCATGCTGGA CATCTTCAAA 
ACCACCATCC CACACATGCT GCTGACTTGG AGACCGGCTG GTGCTGAAGG AGATCACATG 
ACTCAGCTGG AGTCTGATAA ACAGCTGGAC TCCATTTCCC AGAAGTCAGT GGATATTCAG 
ACCCGCTCAG ATGACCAGCA GTCTGTTAAC AGTATGGAGA AGGCTTTGGC TGACAACCGC 
AGATACAGTC AGTTCAGTGT GGCTTCAGAA GCTCAGGAAC AGAGGCTGTT TGCTGCGCTA 
CTCATTAAGT GTGTGGTTCA GCTGGAGCTC ATTCAGACCA TTGACAACAT CGTCTTCTTT 
CCTGCTACCA GTAAGAAAGA AGATGCGGAA AACTTTGCGG CTGCACAGAG AGATGCTTTA 
GATGCTGATA TACATGTGGA CACACAGGAC CAGGGAATGT ATCGCTATCT GACCTCAGAA 
CAGCTCTTCA AACTGCTCGA CTGTCTGCTG GAGTCTCATC GATTTGCAAA GGCCTTCAAC 
TCCAATAATG AGCAAAGAAC TGCTCTGTGG AAAGCCGGTT TCAAAGGGAA GTCGAAGCCC 
AATCTGCTGA AACAGGAGAC GAGCAGCCTG GCGTGTGGTC TGCGCATCCT CTTCCGCATG 
TACACAGATC CAAGCCGACG GGATGCCTGG GAGGAGGTGC AGAGGAGACT GCTCAACGTT 
TGCAGTGACG CAGTGGCTTA TTTTCTGACC CTCACGTCTG AAAGTCATCG CGAAGCCTGG 
ACAAACCTTC TGCTGCTCTT CCTGACCAAA GTGCTGAAGA TCAGTGATGA TAGATTCAAG 
GCTCATGCGT CCCGCTACTA CCCTCTGCTC TGTGAAATCA TGCAGTTCGA CCTGATCCCG 
GAGCTGCGGG CCGTCCTGCG CAAGTTTTTC CTGCGCATTG GACTGGTTTT CCAGATCACC 
AACAGCCTGG AAACAAATCA AACTGTCAGC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295672.1
CDS	206..5764
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATGNMYVTEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGRAGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297397.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297397.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG 
GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT 
CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC 
GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA 
CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC 
TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG 
CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC 
TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT 
AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC 
CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT 
TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA 
ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT 
TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA 
CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG 
AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA 
TACGTCAACC CCAATTCCCA GACAAGTCTT GGGAGAGCAG GCCAAGAAAA GCCTTCAGAG 
CAGGAGAGCA ATGAGTCGAA GCATCCAGAA ACGATCAACC GTTACGGCAG CATTAACTCT 
TTGGACTCCA CTGCATCATC AGGCATTGGG AGCTACAGCA CACAGATGTC CGGAACAGAC 
AACCCGGAGC AGTTTGAGGT CCTCAAACAA CAGAAGGAGA TCATAGAGCA AGGCATTGAC 
CTGTTCAACA AAAAACCAAA GAGGGGCATT CAGTACCTGC AGGAGCAAGG CATGCTGGGA 
ACAACGCCTG AAGATATTGC CCAGTTTTTA CATCAAGAGG AGAGACTTGA CTCTATCCAG 
GTAGGAGAGT TTCTAGGAGA CAACGACCGC ATCAATAAAG AGGTCATGTA TGCCTACGTC 
GACCAAATGG ACTTCCAGGG CAAAGACTTC GTGCCAGCGC TGCGCATGTT CCTGGAGGGT 
TTCCGTCTGC CCGGCGAAGC CCAGAAAATT GATCGACTGA TGGAGAAATT TGCTGCTAGA 
TATTTAGAAT GCAACCAAGG GCAAACACTG TTCGCCAGCG CTGACACCGC ATACGTTCTT 
GCGTATTCAA TCATCATGTT GACTACCGAT CTGCACAGTC CACAGGTGAA GAATAAAATG 
ACGAAAGAGC AATACATCAA GATGAATCGA GGTATCAATG ACAGTAAAGA TTTACCGGAG 
GAGTATCTCT CAGCCATTTA TGATGAAATC GCAGGGAAGA AAATCTCCAT GAAGGAGACA 
AAGGAGTTAA CTCTCAAATC AAATAAGCAA AGTGTGGCGA GTGAGAAGCA GAGGAGGCTG 
CTGTATAATG TTGAGATGGA GCAGATGGCA AAAACAGCAA AGGCTCTGAT GGAGGCCGTG 
AGCCACGTTC AAGCCCCCTT CACCAGCGCC ACACATCTTG AGCATGTCAG ACCAATGTTT 
AAGCTGGCCT GGACGCCCTT TCTGGCTGCT TTCAGTGTGG GTCTGCAGGA CTGTGATGAC 
ACTGAAGTGG CCTCTCTGTG TCTGGAGGGA ATCCGCTGTG CCATCAGGAT AGCATGCATC 
TTCAGCATTC AGTTGGAGCG AGACGCATAT GTACAGGCTC TGGCCCGTTT CACACTCTTG 
ACGGCCAGTT CTGGCATCAC TGAAATGAAG CAGAAGAACA TCGACACCAT CAAGACCCTC 
ATCACAGTGG CTCACACTGA TGGAAATTAT CTAGGAAACT CCTGGCATGA GATCATGAAG 
TGCATTAGTC AGCTGGAGTT GGCCCAGCTC ATCGGGACAG GAGTGAAGGC TCGGTACATA 
TCAGGAACTG TTCGGGGAAA GGAAGGCTTT ATCACCAGCA CCAAGGAACA AACGTCTGAT 
GAATACCTGG GATTGGGAGG GAATGTTGAT CGCAAACAGA TTGCAAGCAT TCAGGAGTCT 
ATCGGGGAGA CCAGCTCGCA AAGTGTAGTT GTTGCTGTAG ACCGGATATT CACGGGCTCT 
ACCAGACTTG ATGGAAATGC CATTGTGGAC TTTGTGCGAT GGCTGTGCGC TGTCTCGATG 
GATGAACTGG CCTCCCCAAC ACACCCTCGA ATGTTCAGTC TGCAGAAGAT CGTGGAGATT 
TCATACTACA ATATGGGTCG TATCAGGTTG CAGTGGTCCA GGATTTGGGA GGTTATAGGG 
GATCATTTCA ATAAGGTTGG CTGTAATCCC AACGAAGATG TTGCTATTTT TGCTGTGGAT 
TCTCTGAGGC AGTTGTCCAT GAAGTTTTTG GAAAAGGGAG AGTTGGCCAA TTTCAGATTC 
CAGAAGGATT TCCTCAGGCC GTTCGAGCAC ATAATGAAAA AGAACAGATC TCCGACCATT 
AGAGACATGG TGGTTCGCTG TATTGCCCAG ATGGTCAACT CTCAGGCTGG CAATATTAGA 
TCAGGGTGGA AGAACATTTT CTCTGTGTTT CATCTGGCTG CTTCTGATCA GGATGAGAGT 
ATAGTTGAGC TGGCCTTCCA GACAACTGGA CACATCGTCA CGAATGTATT TGAGAAGCAT 
TTCCCAGCTA CCATTGACTC CTTCCAGGAC GCTGTCAAGT GTTTGTCTGA GTTCGCCTGC 
AACGCTTCTT TTCCAGACAC CAGCATGGAG GCTATTCGTC TGATCCGCCA CTGCGCCAAA 
TATGTGTCAG ACCGACCCCA GGCGTTTAAA GACTATACCA GTGATGATAT GAATGTTGCC 
CCGGAGGACC GTGTTTGGGT CAGAGGCTGG TTCCCCATCC TTTTTGAGCT GTCATGCATC 
ATCAACAGAT GCAAACTGGA CGTCCGGACC AGGGGTCTGA CAGTGATGTT TGAGGTGATG 
AAGACGTACG GTCATACCTA TGAGAAACAC TGGTGGCAGG ATCTGTTCCG CATCGTCTTC 
AGGATCTTCG ACAACATGAA GCTTCCTGAG CAACAGACCG AGAAAGCGGA GTGGATGACC 
ACCACCTGCA ATCATGCACT TTATGCCATC TGTGACGTTT TTACACAGTA CTTTGAGTCT 
CTCAGTGATG TTCTTTTAGA TGATATCTTA TCTCAGCTCT ACTGGTGTGT CCAGCAAGAT 
AATGAGCAAT TAGCCAGATC AGGCACTAAC TGCCTTGAAA ACGTGGTCAT CCTTAATGGT 
GAGAAGTTCA ATCCTGAGAC TTGGGACAAG ACATGCAACT GCATGCTGGA CATCTTCAAA 
ACCACCATCC CACACATGCT GCTGACTTGG AGACCGGCTG GTGCTGAAGG AGATCACATG 
ACTCAGCTGG AGTCTGATAA ACAGCTGGAC TCCATTTCCC AGAAGTCAGT GGATATTCAG 
ACCCGCTCAG ATGACCAGCA GTCTGTTAAC AGTATGGAGA AGGCTTTGGC TGACAACCGC 
AGATACAGTC AGTTCAGTGT GGCTTCAGAA GCTCAGGAAC AGAGGCTGTT TGCTGCGCTA 
CTCATTAAGT GTGTGGTTCA GCTGGAGCTC ATTCAGACCA TTGACAACAT CGTCTTCTTT 
CCTGCTACCA GTAAGAAAGA AGATGCGGAA AACTTTGCGG CTGCACAGAG AGATGCTTTA 
GATGCTGATA TACATGTGGA CACACAGGAC CAGGGAATGT ATCGCTATCT GACCTCAGAA 
CAGCTCTTCA AACTGCTCGA CTGTCTGCTG GAGTCTCATC GATTTGCAAA GGCCTTCAAC 
TCCAATAATG AGCAAAGAAC TGCTCTGTGG AAAGCCGGTT TCAAAGGGAA GTCGAAGCCC 
AATCTGCTGA AACAGGAGAC GAGCAGCCTG GCGTGTGGTC TGCGCATCCT CTTCCGCATG 
TACACAGATC CAAGCCGACG GGATGCCTGG GAGGAGGTGC AGAGGAGACT GCTCAACGTT 
TGCAGTGACG CAGTGGCTTA TTTTCTGACC CTCACGTCTG AAAGTCATCG CGAAGCCTGG 
ACAAACCTTC TGCTGCTCTT CCTGACCAAA GTGCTGAAGA TCAGTGATGA TAGATTCAAG 
GCTCATGCGT CCCGCTACTA CCCTCTGCTC TGTGAAATCA TGCAGTTCGA CCTGATCCCG 
GAGCTGCGGG CCGTCCTGCG CAAGTTTTTC CTGCGCATTG GACTGGTTTT CCAGATCACC 
AACAGCCTGG AAACAAATCA AACTGTCAGC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_009297398.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295673.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa49266
Clone ID Related Accession (Same CDS sequence)	XM_009297398.1
Accession Version	XM_009297398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGAAATGA AGGCAAGCCA TGAGGGTGAG GCTGAGTCTG CGGAGGTCAG TCCAAACTCT 
CTGGAAGAGG AGGGAGGCTT GGGCAGCGAC AACGAAAATG TTCACGCTAA CGGCATCCCT 
GGCACTCCCA TCTCCGCAAG CTTCACCCCA TCCCTGCCCG ACGACAGGCT GTCTGTCTCT 
TCTAATGACA CTCAGGAATC GGTAGCCACC TCTGGTCAAG CACCAGGCGC AAAGTTTTCT 
CACATTCTCC AGAAAGATGC CTTCCTGGTG TTTCGTTCCC TCTGCAAACT CTCCATGAAG 
CCTCTTTCGG ATGGACCGCC TGATCCCAAA TCCCATGAGC TGCGGTCCAA GGTCCTCTCT 
CTGCAGCTCC TCCTGTCCAT TCTGCAGAAC GCTGGACCCA TCTTCAAGAC CAACGAGATG 
TTCATCAACG CCATCAAGCA GTACCTGTGC GTAGCGCTGT CTAAAAACGG CGTCTCCTCC 
GTCCCCGAGG TGTTCGAGCT GTCTCTGTCC ATCTTCCTCA CCCTCCTGTC CAACTTCAAG 
ACACATCTCA AGATGCAAAT CGAGGTGTTT TTCAAAGAGA TTTTTCTGTA TATTCTGGAA 
ACTTCCACAA GCTCATATGA CCACAAGTGG ATGGTCATTC AAACCCTCAC TAGAATATGT 
GCTGATGCAC AGAGTGTAGT AGATATCTAT GTTAATTATG ATTGTGACCT GAATGCTGCT 
AATATTTTCG AGAGGCTGGT GAATGACCTC TCAAAGATTG CACAAGGACG AGGAGGTCAT 
GAACTGGGTA CAACCCCACT TCAGGAGCTG ACTTTAAGAA AGAAAGGATT GGAGTGTCTG 
GTGTCCATTC TCAAGTGTAT GGTGGAGTGG AGCAAAGACC AATACGTCAA CCCCAATTCC 
CAGACAAGTC TTGGGAGAGC AGGCCAAGAA AAGCCTTCAG AGCAGGAGAG CAATGAGTCG 
AAGCATCCAG AAACGATCAA CCGTTACGGC AGCATTAACT CTTTGGACTC CACTGCATCA 
TCAGGCATTG GGAGCTACAG CACACAGATG TCCGGAACAG ACAACCCGGA GCAGTTTGAG 
GTCCTCAAAC AACAGAAGGA GATCATAGAG CAAGGCATTG ACCTGTTCAA CAAAAAACCA 
AAGAGGGGCA TTCAGTACCT GCAGGAGCAA GGCATGCTGG GAACAACGCC TGAAGATATT 
GCCCAGTTTT TACATCAAGA GGAGAGACTT GACTCTATCC AGGTAGGAGA GTTTCTAGGA 
GACAACGACC GCATCAATAA AGAGGTCATG TATGCCTACG TCGACCAAAT GGACTTCCAG 
GGCAAAGACT TCGTGCCAGC GCTGCGCATG TTCCTGGAGG GTTTCCGTCT GCCCGGCGAA 
GCCCAGAAAA TTGATCGACT GATGGAGAAA TTTGCTGCTA GATATTTAGA ATGCAACCAA 
GGGCAAACAC TGTTCGCCAG CGCTGACACC GCATACGTTC TTGCGTATTC AATCATCATG 
TTGACTACCG ATCTGCACAG TCCACAGGTG AAGAATAAAA TGACGAAAGA GCAATACATC 
AAGATGAATC GAGGTATCAA TGACAGTAAA GATTTACCGG AGGAGTATCT CTCAGCCATT 
TATGATGAAA TCGCAGGGAA GAAAATCTCC ATGAAGGAGA CAAAGGAGTT AACTCTCAAA 
TCAAATAAGC AAAGTGTGGC GAGTGAGAAG CAGAGGAGGC TGCTGTATAA TGTTGAGATG 
GAGCAGATGG CAAAAACAGC AAAGGCTCTG ATGGAGGCCG TGAGCCACGT TCAAGCCCCC 
TTCACCAGCG CCACACATCT TGAGCATGTC AGACCAATGT TTAAGCTGGC CTGGACGCCC 
TTTCTGGCTG CTTTCAGTGT GGGTCTGCAG GACTGTGATG ACACTGAAGT GGCCTCTCTG 
TGTCTGGAGG GAATCCGCTG TGCCATCAGG ATAGCATGCA TCTTCAGCAT TCAGTTGGAG 
CGAGACGCAT ATGTACAGGC TCTGGCCCGT TTCACACTCT TGACGGCCAG TTCTGGCATC 
ACTGAAATGA AGCAGAAGAA CATCGACACC ATCAAGACCC TCATCACAGT GGCTCACACT 
GATGGAAATT ATCTAGGAAA CTCCTGGCAT GAGATCATGA AGTGCATTAG TCAGCTGGAG 
TTGGCCCAGC TCATCGGGAC AGGAGTGAAG GCTCGGTACA TATCAGGAAC TGTTCGGGGA 
AAGGAAGGCT TTATCACCAG CACCAAGGAA CAAACGTCTG ATGAATACCT GGGATTGGGA 
GGGAATGTTG ATCGCAAACA GATTGCAAGC ATTCAGGAGT CTATCGGGGA GACCAGCTCG 
CAAAGTGTAG TTGTTGCTGT AGACCGGATA TTCACGGGCT CTACCAGACT TGATGGAAAT 
GCCATTGTGG ACTTTGTGCG ATGGCTGTGC GCTGTCTCGA TGGATGAACT GGCCTCCCCA 
ACACACCCTC GAATGTTCAG TCTGCAGAAG ATCGTGGAGA TTTCATACTA CAATATGGGT 
CGTATCAGGT TGCAGTGGTC CAGGATTTGG GAGGTTATAG GGGATCATTT CAATAAGGTT 
GGCTGTAATC CCAACGAAGA TGTTGCTATT TTTGCTGTGG ATTCTCTGAG GCAGTTGTCC 
ATGAAGTTTT TGGAAAAGGG AGAGTTGGCC AATTTCAGAT TCCAGAAGGA TTTCCTCAGG 
CCGTTCGAGC ACATAATGAA AAAGAACAGA TCTCCGACCA TTAGAGACAT GGTGGTTCGC 
TGTATTGCCC AGATGGTCAA CTCTCAGGCT GGCAATATTA GATCAGGGTG GAAGAACATT 
TTCTCTGTGT TTCATCTGGC TGCTTCTGAT CAGGATGAGA GTATAGTTGA GCTGGCCTTC 
CAGACAACTG GACACATCGT CACGAATGTA TTTGAGAAGC ATTTCCCAGC TACCATTGAC 
TCCTTCCAGG ACGCTGTCAA GTGTTTGTCT GAGTTCGCCT GCAACGCTTC TTTTCCAGAC 
ACCAGCATGG AGGCTATTCG TCTGATCCGC CACTGCGCCA AATATGTGTC AGACCGACCC 
CAGGCGTTTA AAGACTATAC CAGTGATGAT ATGAATGTTG CCCCGGAGGA CCGTGTTTGG 
GTCAGAGGCT GGTTCCCCAT CCTTTTTGAG CTGTCATGCA TCATCAACAG ATGCAAACTG 
GACGTCCGGA CCAGGGGTCT GACAGTGATG TTTGAGGTGA TGAAGACGTA CGGTCATACC 
TATGAGAAAC ACTGGTGGCA GGATCTGTTC CGCATCGTCT TCAGGATCTT CGACAACATG 
AAGCTTCCTG AGCAACAGAC CGAGAAAGCG GAGTGGATGA CCACCACCTG CAATCATGCA 
CTTTATGCCA TCTGTGACGT TTTTACACAG TACTTTGAGT CTCTCAGTGA TGTTCTTTTA 
GATGATATCT TATCTCAGCT CTACTGGTGT GTCCAGCAAG ATAATGAGCA ATTAGCCAGA 
TCAGGCACTA ACTGCCTTGA AAACGTGGTC ATCCTTAATG GTGAGAAGTT CAATCCTGAG 
ACTTGGGACA AGACATGCAA CTGCATGCTG GACATCTTCA AAACCACCAT CCCACACATG 
CTGCTGACTT GGAGACCGGC TGGTGCTGAA GGAGATCACA TGACTCAGCT GGAGTCTGAT 
AAACAGCTGG ACTCCATTTC CCAGAAGTCA GTGGATATTC AGACCCGCTC AGATGACCAG 
CAGTCTGTTA ACAGTATGGA GAAGGCTTTG GCTGACAACC GCAGATACAG TCAGTTCAGT 
GTGGCTTCAG AAGCTCAGGA ACAGAGGCTG TTTGCTGCGC TACTCATTAA GTGTGTGGTT 
CAGCTGGAGC TCATTCAGAC CATTGACAAC ATCGTCTTCT TTCCTGCTAC CAGTAAGAAA 
GAAGATGCGG AAAACTTTGC GGCTGCACAG AGAGATGCTT TAGATGCTGA TATACATGTG 
GACACACAGG ACCAGGGAAT GTATCGCTAT CTGACCTCAG AACAGCTCTT CAAACTGCTC 
GACTGTCTGC TGGAGTCTCA TCGATTTGCA AAGGCCTTCA ACTCCAATAA TGAGCAAAGA 
ACTGCTCTGT GGAAAGCCGG TTTCAAAGGG AAGTCGAAGC CCAATCTGCT GAAACAGGAG 
ACGAGCAGCC TGGCGTGTGG TCTGCGCATC CTCTTCCGCA TGTACACAGA TCCAAGCCGA 
CGGGATGCCT GGGAGGAGGT GCAGAGGAGA CTGCTCAACG TTTGCAGTGA CGCAGTGGCT 
TATTTTCTGA CCCTCACGTC TGAAAGTCAT CGCGAAGCCT GGACAAACCT TCTGCTGCTC 
TTCCTGACCA AAGTGCTGAA GATCAGTGAT GATAGATTCA AGGCTCATGC GTCCCGCTAC 
TACCCTCTGC TCTGTGAAAT CATGCAGTTC GACCTGATCC CGGAGCTGCG GGCCGTCCTG 
CGCAAGTTTT TCCTGCGCAT TGGACTGGTT TTCCAGATCA CCAACAGCCT GGAAACAAAT 
CAAACTGTCA GCAACGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295673.1
CDS	206..5746
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGRAGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297398.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297398.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGAAATGA AGGCAAGCCA TGAGGGTGAG GCTGAGTCTG CGGAGGTCAG TCCAAACTCT 
CTGGAAGAGG AGGGAGGCTT GGGCAGCGAC AACGAAAATG TTCACGCTAA CGGCATCCCT 
GGCACTCCCA TCTCCGCAAG CTTCACCCCA TCCCTGCCCG ACGACAGGCT GTCTGTCTCT 
TCTAATGACA CTCAGGAATC GGTAGCCACC TCTGGTCAAG CACCAGGCGC AAAGTTTTCT 
CACATTCTCC AGAAAGATGC CTTCCTGGTG TTTCGTTCCC TCTGCAAACT CTCCATGAAG 
CCTCTTTCGG ATGGACCGCC TGATCCCAAA TCCCATGAGC TGCGGTCCAA GGTCCTCTCT 
CTGCAGCTCC TCCTGTCCAT TCTGCAGAAC GCTGGACCCA TCTTCAAGAC CAACGAGATG 
TTCATCAACG CCATCAAGCA GTACCTGTGC GTAGCGCTGT CTAAAAACGG CGTCTCCTCC 
GTCCCCGAGG TGTTCGAGCT GTCTCTGTCC ATCTTCCTCA CCCTCCTGTC CAACTTCAAG 
ACACATCTCA AGATGCAAAT CGAGGTGTTT TTCAAAGAGA TTTTTCTGTA TATTCTGGAA 
ACTTCCACAA GCTCATATGA CCACAAGTGG ATGGTCATTC AAACCCTCAC TAGAATATGT 
GCTGATGCAC AGAGTGTAGT AGATATCTAT GTTAATTATG ATTGTGACCT GAATGCTGCT 
AATATTTTCG AGAGGCTGGT GAATGACCTC TCAAAGATTG CACAAGGACG AGGAGGTCAT 
GAACTGGGTA CAACCCCACT TCAGGAGCTG ACTTTAAGAA AGAAAGGATT GGAGTGTCTG 
GTGTCCATTC TCAAGTGTAT GGTGGAGTGG AGCAAAGACC AATACGTCAA CCCCAATTCC 
CAGACAAGTC TTGGGAGAGC AGGCCAAGAA AAGCCTTCAG AGCAGGAGAG CAATGAGTCG 
AAGCATCCAG AAACGATCAA CCGTTACGGC AGCATTAACT CTTTGGACTC CACTGCATCA 
TCAGGCATTG GGAGCTACAG CACACAGATG TCCGGAACAG ACAACCCGGA GCAGTTTGAG 
GTCCTCAAAC AACAGAAGGA GATCATAGAG CAAGGCATTG ACCTGTTCAA CAAAAAACCA 
AAGAGGGGCA TTCAGTACCT GCAGGAGCAA GGCATGCTGG GAACAACGCC TGAAGATATT 
GCCCAGTTTT TACATCAAGA GGAGAGACTT GACTCTATCC AGGTAGGAGA GTTTCTAGGA 
GACAACGACC GCATCAATAA AGAGGTCATG TATGCCTACG TCGACCAAAT GGACTTCCAG 
GGCAAAGACT TCGTGCCAGC GCTGCGCATG TTCCTGGAGG GTTTCCGTCT GCCCGGCGAA 
GCCCAGAAAA TTGATCGACT GATGGAGAAA TTTGCTGCTA GATATTTAGA ATGCAACCAA 
GGGCAAACAC TGTTCGCCAG CGCTGACACC GCATACGTTC TTGCGTATTC AATCATCATG 
TTGACTACCG ATCTGCACAG TCCACAGGTG AAGAATAAAA TGACGAAAGA GCAATACATC 
AAGATGAATC GAGGTATCAA TGACAGTAAA GATTTACCGG AGGAGTATCT CTCAGCCATT 
TATGATGAAA TCGCAGGGAA GAAAATCTCC ATGAAGGAGA CAAAGGAGTT AACTCTCAAA 
TCAAATAAGC AAAGTGTGGC GAGTGAGAAG CAGAGGAGGC TGCTGTATAA TGTTGAGATG 
GAGCAGATGG CAAAAACAGC AAAGGCTCTG ATGGAGGCCG TGAGCCACGT TCAAGCCCCC 
TTCACCAGCG CCACACATCT TGAGCATGTC AGACCAATGT TTAAGCTGGC CTGGACGCCC 
TTTCTGGCTG CTTTCAGTGT GGGTCTGCAG GACTGTGATG ACACTGAAGT GGCCTCTCTG 
TGTCTGGAGG GAATCCGCTG TGCCATCAGG ATAGCATGCA TCTTCAGCAT TCAGTTGGAG 
CGAGACGCAT ATGTACAGGC TCTGGCCCGT TTCACACTCT TGACGGCCAG TTCTGGCATC 
ACTGAAATGA AGCAGAAGAA CATCGACACC ATCAAGACCC TCATCACAGT GGCTCACACT 
GATGGAAATT ATCTAGGAAA CTCCTGGCAT GAGATCATGA AGTGCATTAG TCAGCTGGAG 
TTGGCCCAGC TCATCGGGAC AGGAGTGAAG GCTCGGTACA TATCAGGAAC TGTTCGGGGA 
AAGGAAGGCT TTATCACCAG CACCAAGGAA CAAACGTCTG ATGAATACCT GGGATTGGGA 
GGGAATGTTG ATCGCAAACA GATTGCAAGC ATTCAGGAGT CTATCGGGGA GACCAGCTCG 
CAAAGTGTAG TTGTTGCTGT AGACCGGATA TTCACGGGCT CTACCAGACT TGATGGAAAT 
GCCATTGTGG ACTTTGTGCG ATGGCTGTGC GCTGTCTCGA TGGATGAACT GGCCTCCCCA 
ACACACCCTC GAATGTTCAG TCTGCAGAAG ATCGTGGAGA TTTCATACTA CAATATGGGT 
CGTATCAGGT TGCAGTGGTC CAGGATTTGG GAGGTTATAG GGGATCATTT CAATAAGGTT 
GGCTGTAATC CCAACGAAGA TGTTGCTATT TTTGCTGTGG ATTCTCTGAG GCAGTTGTCC 
ATGAAGTTTT TGGAAAAGGG AGAGTTGGCC AATTTCAGAT TCCAGAAGGA TTTCCTCAGG 
CCGTTCGAGC ACATAATGAA AAAGAACAGA TCTCCGACCA TTAGAGACAT GGTGGTTCGC 
TGTATTGCCC AGATGGTCAA CTCTCAGGCT GGCAATATTA GATCAGGGTG GAAGAACATT 
TTCTCTGTGT TTCATCTGGC TGCTTCTGAT CAGGATGAGA GTATAGTTGA GCTGGCCTTC 
CAGACAACTG GACACATCGT CACGAATGTA TTTGAGAAGC ATTTCCCAGC TACCATTGAC 
TCCTTCCAGG ACGCTGTCAA GTGTTTGTCT GAGTTCGCCT GCAACGCTTC TTTTCCAGAC 
ACCAGCATGG AGGCTATTCG TCTGATCCGC CACTGCGCCA AATATGTGTC AGACCGACCC 
CAGGCGTTTA AAGACTATAC CAGTGATGAT ATGAATGTTG CCCCGGAGGA CCGTGTTTGG 
GTCAGAGGCT GGTTCCCCAT CCTTTTTGAG CTGTCATGCA TCATCAACAG ATGCAAACTG 
GACGTCCGGA CCAGGGGTCT GACAGTGATG TTTGAGGTGA TGAAGACGTA CGGTCATACC 
TATGAGAAAC ACTGGTGGCA GGATCTGTTC CGCATCGTCT TCAGGATCTT CGACAACATG 
AAGCTTCCTG AGCAACAGAC CGAGAAAGCG GAGTGGATGA CCACCACCTG CAATCATGCA 
CTTTATGCCA TCTGTGACGT TTTTACACAG TACTTTGAGT CTCTCAGTGA TGTTCTTTTA 
GATGATATCT TATCTCAGCT CTACTGGTGT GTCCAGCAAG ATAATGAGCA ATTAGCCAGA 
TCAGGCACTA ACTGCCTTGA AAACGTGGTC ATCCTTAATG GTGAGAAGTT CAATCCTGAG 
ACTTGGGACA AGACATGCAA CTGCATGCTG GACATCTTCA AAACCACCAT CCCACACATG 
CTGCTGACTT GGAGACCGGC TGGTGCTGAA GGAGATCACA TGACTCAGCT GGAGTCTGAT 
AAACAGCTGG ACTCCATTTC CCAGAAGTCA GTGGATATTC AGACCCGCTC AGATGACCAG 
CAGTCTGTTA ACAGTATGGA GAAGGCTTTG GCTGACAACC GCAGATACAG TCAGTTCAGT 
GTGGCTTCAG AAGCTCAGGA ACAGAGGCTG TTTGCTGCGC TACTCATTAA GTGTGTGGTT 
CAGCTGGAGC TCATTCAGAC CATTGACAAC ATCGTCTTCT TTCCTGCTAC CAGTAAGAAA 
GAAGATGCGG AAAACTTTGC GGCTGCACAG AGAGATGCTT TAGATGCTGA TATACATGTG 
GACACACAGG ACCAGGGAAT GTATCGCTAT CTGACCTCAG AACAGCTCTT CAAACTGCTC 
GACTGTCTGC TGGAGTCTCA TCGATTTGCA AAGGCCTTCA ACTCCAATAA TGAGCAAAGA 
ACTGCTCTGT GGAAAGCCGG TTTCAAAGGG AAGTCGAAGC CCAATCTGCT GAAACAGGAG 
ACGAGCAGCC TGGCGTGTGG TCTGCGCATC CTCTTCCGCA TGTACACAGA TCCAAGCCGA 
CGGGATGCCT GGGAGGAGGT GCAGAGGAGA CTGCTCAACG TTTGCAGTGA CGCAGTGGCT 
TATTTTCTGA CCCTCACGTC TGAAAGTCAT CGCGAAGCCT GGACAAACCT TCTGCTGCTC 
TTCCTGACCA AAGTGCTGAA GATCAGTGAT GATAGATTCA AGGCTCATGC GTCCCGCTAC 
TACCCTCTGC TCTGTGAAAT CATGCAGTTC GACCTGATCC CGGAGCTGCG GGCCGTCCTG 
CGCAAGTTTT TCCTGCGCAT TGGACTGGTT TTCCAGATCA CCAACAGCCT GGAAACAAAT 
CAAACTGTCA GCAACGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_009297399.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295674.1 Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X6, mRNA.

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CloneID	ODa49267
Clone ID Related Accession (Same CDS sequence)	XM_009297399.1
Accession Version	XM_009297399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG 
GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT 
CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC 
GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA 
CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC 
TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG 
CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC 
TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT 
AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC 
CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT 
TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA 
ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT 
TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA 
CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG 
AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA 
TACGTCAACC CCAATTCCCA GACAAGTCTT GGCCAAGAAA AGCCTTCAGA GCAGGAGAGC 
AATGAGTCGA AGCATCCAGA AACGATCAAC CGTTACGGCA GCATTAACTC TTTGGACTCC 
ACTGCATCAT CAGGCATTGG GAGCTACAGC ACACAGATGT CCGGAACAGA CAACCCGGAG 
CAGTTTGAGG TCCTCAAACA ACAGAAGGAG ATCATAGAGC AAGGCATTGA CCTGTTCAAC 
AAAAAACCAA AGAGGGGCAT TCAGTACCTG CAGGAGCAAG GCATGCTGGG AACAACGCCT 
GAAGATATTG CCCAGTTTTT ACATCAAGAG GAGAGACTTG ACTCTATCCA GGTAGGAGAG 
TTTCTAGGAG ACAACGACCG CATCAATAAA GAGGTCATGT ATGCCTACGT CGACCAAATG 
GACTTCCAGG GCAAAGACTT CGTGCCAGCG CTGCGCATGT TCCTGGAGGG TTTCCGTCTG 
CCCGGCGAAG CCCAGAAAAT TGATCGACTG ATGGAGAAAT TTGCTGCTAG ATATTTAGAA 
TGCAACCAAG GGCAAACACT GTTCGCCAGC GCTGACACCG CATACGTTCT TGCGTATTCA 
ATCATCATGT TGACTACCGA TCTGCACAGT CCACAGGTGA AGAATAAAAT GACGAAAGAG 
CAATACATCA AGATGAATCG AGGTATCAAT GACAGTAAAG ATTTACCGGA GGAGTATCTC 
TCAGCCATTT ATGATGAAAT CGCAGGGAAG AAAATCTCCA TGAAGGAGAC AAAGGAGTTA 
ACTCTCAAAT CAAATAAGCA AAGTGTGGCG AGTGAGAAGC AGAGGAGGCT GCTGTATAAT 
GTTGAGATGG AGCAGATGGC AAAAACAGCA AAGGCTCTGA TGGAGGCCGT GAGCCACGTT 
CAAGCCCCCT TCACCAGCGC CACACATCTT GAGCATGTCA GACCAATGTT TAAGCTGGCC 
TGGACGCCCT TTCTGGCTGC TTTCAGTGTG GGTCTGCAGG ACTGTGATGA CACTGAAGTG 
GCCTCTCTGT GTCTGGAGGG AATCCGCTGT GCCATCAGGA TAGCATGCAT CTTCAGCATT 
CAGTTGGAGC GAGACGCATA TGTACAGGCT CTGGCCCGTT TCACACTCTT GACGGCCAGT 
TCTGGCATCA CTGAAATGAA GCAGAAGAAC ATCGACACCA TCAAGACCCT CATCACAGTG 
GCTCACACTG ATGGAAATTA TCTAGGAAAC TCCTGGCATG AGATCATGAA GTGCATTAGT 
CAGCTGGAGT TGGCCCAGCT CATCGGGACA GGAGTGAAGG CTCGGTACAT ATCAGGAACT 
GTTCGGGGAA AGGAAGGCTT TATCACCAGC ACCAAGGAAC AAACGTCTGA TGAATACCTG 
GGATTGGGAG GGAATGTTGA TCGCAAACAG ATTGCAAGCA TTCAGGAGTC TATCGGGGAG 
ACCAGCTCGC AAAGTGTAGT TGTTGCTGTA GACCGGATAT TCACGGGCTC TACCAGACTT 
GATGGAAATG CCATTGTGGA CTTTGTGCGA TGGCTGTGCG CTGTCTCGAT GGATGAACTG 
GCCTCCCCAA CACACCCTCG AATGTTCAGT CTGCAGAAGA TCGTGGAGAT TTCATACTAC 
AATATGGGTC GTATCAGGTT GCAGTGGTCC AGGATTTGGG AGGTTATAGG GGATCATTTC 
AATAAGGTTG GCTGTAATCC CAACGAAGAT GTTGCTATTT TTGCTGTGGA TTCTCTGAGG 
CAGTTGTCCA TGAAGTTTTT GGAAAAGGGA GAGTTGGCCA ATTTCAGATT CCAGAAGGAT 
TTCCTCAGGC CGTTCGAGCA CATAATGAAA AAGAACAGAT CTCCGACCAT TAGAGACATG 
GTGGTTCGCT GTATTGCCCA GATGGTCAAC TCTCAGGCTG GCAATATTAG ATCAGGGTGG 
AAGAACATTT TCTCTGTGTT TCATCTGGCT GCTTCTGATC AGGATGAGAG TATAGTTGAG 
CTGGCCTTCC AGACAACTGG ACACATCGTC ACGAATGTAT TTGAGAAGCA TTTCCCAGCT 
ACCATTGACT CCTTCCAGGA CGCTGTCAAG TGTTTGTCTG AGTTCGCCTG CAACGCTTCT 
TTTCCAGACA CCAGCATGGA GGCTATTCGT CTGATCCGCC ACTGCGCCAA ATATGTGTCA 
GACCGACCCC AGGCGTTTAA AGACTATACC AGTGATGATA TGAATGTTGC CCCGGAGGAC 
CGTGTTTGGG TCAGAGGCTG GTTCCCCATC CTTTTTGAGC TGTCATGCAT CATCAACAGA 
TGCAAACTGG ACGTCCGGAC CAGGGGTCTG ACAGTGATGT TTGAGGTGAT GAAGACGTAC 
GGTCATACCT ATGAGAAACA CTGGTGGCAG GATCTGTTCC GCATCGTCTT CAGGATCTTC 
GACAACATGA AGCTTCCTGA GCAACAGACC GAGAAAGCGG AGTGGATGAC CACCACCTGC 
AATCATGCAC TTTATGCCAT CTGTGACGTT TTTACACAGT ACTTTGAGTC TCTCAGTGAT 
GTTCTTTTAG ATGATATCTT ATCTCAGCTC TACTGGTGTG TCCAGCAAGA TAATGAGCAA 
TTAGCCAGAT CAGGCACTAA CTGCCTTGAA AACGTGGTCA TCCTTAATGG TGAGAAGTTC 
AATCCTGAGA CTTGGGACAA GACATGCAAC TGCATGCTGG ACATCTTCAA AACCACCATC 
CCACACATGC TGCTGACTTG GAGACCGGCT GGTGCTGAAG GAGATCACAT GACTCAGCTG 
GAGTCTGATA AACAGCTGGA CTCCATTTCC CAGAAGTCAG TGGATATTCA GACCCGCTCA 
GATGACCAGC AGTCTGTTAA CAGTATGGAG AAGGCTTTGG CTGACAACCG CAGATACAGT 
CAGTTCAGTG TGGCTTCAGA AGCTCAGGAA CAGAGGCTGT TTGCTGCGCT ACTCATTAAG 
TGTGTGGTTC AGCTGGAGCT CATTCAGACC ATTGACAACA TCGTCTTCTT TCCTGCTACC 
AGTAAGAAAG AAGATGCGGA AAACTTTGCG GCTGCACAGA GAGATGCTTT AGATGCTGAT 
ATACATGTGG ACACACAGGA CCAGGGAATG TATCGCTATC TGACCTCAGA ACAGCTCTTC 
AAACTGCTCG ACTGTCTGCT GGAGTCTCAT CGATTTGCAA AGGCCTTCAA CTCCAATAAT 
GAGCAAAGAA CTGCTCTGTG GAAAGCCGGT TTCAAAGGGA AGTCGAAGCC CAATCTGCTG 
AAACAGGAGA CGAGCAGCCT GGCGTGTGGT CTGCGCATCC TCTTCCGCAT GTACACAGAT 
CCAAGCCGAC GGGATGCCTG GGAGGAGGTG CAGAGGAGAC TGCTCAACGT TTGCAGTGAC 
GCAGTGGCTT ATTTTCTGAC CCTCACGTCT GAAAGTCATC GCGAAGCCTG GACAAACCTT 
CTGCTGCTCT TCCTGACCAA AGTGCTGAAG ATCAGTGATG ATAGATTCAA GGCTCATGCG 
TCCCGCTACT ACCCTCTGCT CTGTGAAATC ATGCAGTTCG ACCTGATCCC GGAGCTGCGG 
GCCGTCCTGC GCAAGTTTTT CCTGCGCATT GGACTGGTTT TCCAGATCAC CAACAGCCTG 
GAAACAAATC AAACTGTCAG CAACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295674.1
CDS	206..5755
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKQESEKLSSPVGDNKSSSSTLPPIKSKSSFIEADKYFLPFELACQSKCPRIVITSLDCLQKLIAYGHLTGSAPDNTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQHSPVSQQEPESPGQRHTETPGKTLPAEGNGGPGHQGGKVEQGSPPYESPEPENGSDFCPAENEQTEADQATAAAQSAAEHATEASEEDERQNYEEKAQEIVESVLLEVVNTVATGNMYVTEMKASHEGEAESAEVSPNSLEEEGGLGSDNENVHANGIPGTPISASFTPSLPDDRLSVSSNDTQESVATSGQAPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSYDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGGHELGTTPLQELTLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGQEKPSEQESNESKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSIQVGEFLGDNDRINKEVMYAYVDQMDFQGKDFVPALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTLKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEIMKCISQLELAQLIGTGVKARYISGTVRGKEGFITSTKEQTSDEYLGLGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSDRPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFESLSDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFNPETWDKTCNCMLDIFKTTIPHMLLTWRPAGAEGDHMTQLESDKQLDSISQKSVDIQTRSDDQQSVNSMEKALADNRRYSQFSVASEAQEQRLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENFAAAQRDALDADIHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDPSRRDAWEEVQRRLLNVCSDAVAYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFFLRIGLVFQITNSLETNQTVSNA

Target ORF information:

RefSeq Version	XM_009297399.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (arfgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297399.1

ORF Insert Sequence:

ATGTATGAAG GCAAAAAGAC GAAAAACATG TTTTTAACCA GAGCTTTGGA GAAGATCTTG 
GCCGACAAGG AGGTGAAAAA GGCTCATCAC TCGCAGCTGC GCAAAGCCTG TGAGGTGGCA 
TTAGAGGAAA TCAAGCAGGA ATCGGAGAAA TTAAGCTCAC CGGTCGGCGA CAACAAGTCG 
AGTTCCAGCA CTTTGCCACC AATCAAGTCG AAGAGCAGCT TCATTGAAGC AGATAAATAC 
TTCCTGCCAT TTGAGTTGGC CTGTCAGTCC AAATGTCCTC GCATCGTCAT CACCTCTCTA 
GACTGTTTAC AGAAACTCAT AGCGTATGGT CACCTGACGG GCAGCGCTCC AGACAACACC 
ACACCAGGGA AGAAACTCAT CGACAGGATC ATAGAGACTA TATGTGGCTG CTTTCAGGGC 
CCGCAGACAG ACGAAGGAGT CCAGCTACAG ATTATAAAGG CCTTGCTGAC AGCGGTGACA 
TCCCAGCAAA TAGAGATCCA TGAGGGCACA GTCTTACAGG CCGTCAGAAC ATGCTACAAC 
ATCTATCTGG CCAGTAAAAA CCTCATCAAC CAGACCACGG CCAAAGCCAC CCTCACCCAG 
ATGCTGAATG TCATATTCGC CCGCATGGAG AACCAAGCAC TGCAGGAAGC CAAGCAGATG 
GAGAGAGAGC GCCACAGACA GCATTCTCCT GTCAGTCAGC AAGAGCCTGA ATCTCCTGGA 
CAGAGGCACA CCGAGACCCC TGGCAAAACC CTGCCAGCTG AGGGCAACGG TGGACCTGGA 
CATCAGGGTG GTAAAGTGGA GCAGGGTTCC CCACCGTATG AGAGTCCCGA GCCAGAGAAT 
GGAAGTGACT TCTGTCCTGC GGAGAATGAG CAGACCGAGG CTGACCAGGC CACCGCTGCA 
GCACAAAGTG CAGCAGAGCA TGCCACTGAA GCCAGCGAGG AGGATGAAAG GCAGAACTAT 
GAAGAGAAGG CGCAGGAGAT TGTGGAGAGC GTTTTGCTAG AAGTGGTGAA CACAGTGGCT 
ACAGGAAACA TGTATGTTAC AGAAATGAAG GCAAGCCATG AGGGTGAGGC TGAGTCTGCG 
GAGGTCAGTC CAAACTCTCT GGAAGAGGAG GGAGGCTTGG GCAGCGACAA CGAAAATGTT 
CACGCTAACG GCATCCCTGG CACTCCCATC TCCGCAAGCT TCACCCCATC CCTGCCCGAC 
GACAGGCTGT CTGTCTCTTC TAATGACACT CAGGAATCGG TAGCCACCTC TGGTCAAGCA 
CCAGGCGCAA AGTTTTCTCA CATTCTCCAG AAAGATGCCT TCCTGGTGTT TCGTTCCCTC 
TGCAAACTCT CCATGAAGCC TCTTTCGGAT GGACCGCCTG ATCCCAAATC CCATGAGCTG 
CGGTCCAAGG TCCTCTCTCT GCAGCTCCTC CTGTCCATTC TGCAGAACGC TGGACCCATC 
TTCAAGACCA ACGAGATGTT CATCAACGCC ATCAAGCAGT ACCTGTGCGT AGCGCTGTCT 
AAAAACGGCG TCTCCTCCGT CCCCGAGGTG TTCGAGCTGT CTCTGTCCAT CTTCCTCACC 
CTCCTGTCCA ACTTCAAGAC ACATCTCAAG ATGCAAATCG AGGTGTTTTT CAAAGAGATT 
TTTCTGTATA TTCTGGAAAC TTCCACAAGC TCATATGACC ACAAGTGGAT GGTCATTCAA 
ACCCTCACTA GAATATGTGC TGATGCACAG AGTGTAGTAG ATATCTATGT TAATTATGAT 
TGTGACCTGA ATGCTGCTAA TATTTTCGAG AGGCTGGTGA ATGACCTCTC AAAGATTGCA 
CAAGGACGAG GAGGTCATGA ACTGGGTACA ACCCCACTTC AGGAGCTGAC TTTAAGAAAG 
AAAGGATTGG AGTGTCTGGT GTCCATTCTC AAGTGTATGG TGGAGTGGAG CAAAGACCAA 
TACGTCAACC CCAATTCCCA GACAAGTCTT GGCCAAGAAA AGCCTTCAGA GCAGGAGAGC 
AATGAGTCGA AGCATCCAGA AACGATCAAC CGTTACGGCA GCATTAACTC TTTGGACTCC 
ACTGCATCAT CAGGCATTGG GAGCTACAGC ACACAGATGT CCGGAACAGA CAACCCGGAG 
CAGTTTGAGG TCCTCAAACA ACAGAAGGAG ATCATAGAGC AAGGCATTGA CCTGTTCAAC 
AAAAAACCAA AGAGGGGCAT TCAGTACCTG CAGGAGCAAG GCATGCTGGG AACAACGCCT 
GAAGATATTG CCCAGTTTTT ACATCAAGAG GAGAGACTTG ACTCTATCCA GGTAGGAGAG 
TTTCTAGGAG ACAACGACCG CATCAATAAA GAGGTCATGT ATGCCTACGT CGACCAAATG 
GACTTCCAGG GCAAAGACTT CGTGCCAGCG CTGCGCATGT TCCTGGAGGG TTTCCGTCTG 
CCCGGCGAAG CCCAGAAAAT TGATCGACTG ATGGAGAAAT TTGCTGCTAG ATATTTAGAA 
TGCAACCAAG GGCAAACACT GTTCGCCAGC GCTGACACCG CATACGTTCT TGCGTATTCA 
ATCATCATGT TGACTACCGA TCTGCACAGT CCACAGGTGA AGAATAAAAT GACGAAAGAG 
CAATACATCA AGATGAATCG AGGTATCAAT GACAGTAAAG ATTTACCGGA GGAGTATCTC 
TCAGCCATTT ATGATGAAAT CGCAGGGAAG AAAATCTCCA TGAAGGAGAC AAAGGAGTTA 
ACTCTCAAAT CAAATAAGCA AAGTGTGGCG AGTGAGAAGC AGAGGAGGCT GCTGTATAAT 
GTTGAGATGG AGCAGATGGC AAAAACAGCA AAGGCTCTGA TGGAGGCCGT GAGCCACGTT 
CAAGCCCCCT TCACCAGCGC CACACATCTT GAGCATGTCA GACCAATGTT TAAGCTGGCC 
TGGACGCCCT TTCTGGCTGC TTTCAGTGTG GGTCTGCAGG ACTGTGATGA CACTGAAGTG 
GCCTCTCTGT GTCTGGAGGG AATCCGCTGT GCCATCAGGA TAGCATGCAT CTTCAGCATT 
CAGTTGGAGC GAGACGCATA TGTACAGGCT CTGGCCCGTT TCACACTCTT GACGGCCAGT 
TCTGGCATCA CTGAAATGAA GCAGAAGAAC ATCGACACCA TCAAGACCCT CATCACAGTG 
GCTCACACTG ATGGAAATTA TCTAGGAAAC TCCTGGCATG AGATCATGAA GTGCATTAGT 
CAGCTGGAGT TGGCCCAGCT CATCGGGACA GGAGTGAAGG CTCGGTACAT ATCAGGAACT 
GTTCGGGGAA AGGAAGGCTT TATCACCAGC ACCAAGGAAC AAACGTCTGA TGAATACCTG 
GGATTGGGAG GGAATGTTGA TCGCAAACAG ATTGCAAGCA TTCAGGAGTC TATCGGGGAG 
ACCAGCTCGC AAAGTGTAGT TGTTGCTGTA GACCGGATAT TCACGGGCTC TACCAGACTT 
GATGGAAATG CCATTGTGGA CTTTGTGCGA TGGCTGTGCG CTGTCTCGAT GGATGAACTG 
GCCTCCCCAA CACACCCTCG AATGTTCAGT CTGCAGAAGA TCGTGGAGAT TTCATACTAC 
AATATGGGTC GTATCAGGTT GCAGTGGTCC AGGATTTGGG AGGTTATAGG GGATCATTTC 
AATAAGGTTG GCTGTAATCC CAACGAAGAT GTTGCTATTT TTGCTGTGGA TTCTCTGAGG 
CAGTTGTCCA TGAAGTTTTT GGAAAAGGGA GAGTTGGCCA ATTTCAGATT CCAGAAGGAT 
TTCCTCAGGC CGTTCGAGCA CATAATGAAA AAGAACAGAT CTCCGACCAT TAGAGACATG 
GTGGTTCGCT GTATTGCCCA GATGGTCAAC TCTCAGGCTG GCAATATTAG ATCAGGGTGG 
AAGAACATTT TCTCTGTGTT TCATCTGGCT GCTTCTGATC AGGATGAGAG TATAGTTGAG 
CTGGCCTTCC AGACAACTGG ACACATCGTC ACGAATGTAT TTGAGAAGCA TTTCCCAGCT 
ACCATTGACT CCTTCCAGGA CGCTGTCAAG TGTTTGTCTG AGTTCGCCTG CAACGCTTCT 
TTTCCAGACA CCAGCATGGA GGCTATTCGT CTGATCCGCC ACTGCGCCAA ATATGTGTCA 
GACCGACCCC AGGCGTTTAA AGACTATACC AGTGATGATA TGAATGTTGC CCCGGAGGAC 
CGTGTTTGGG TCAGAGGCTG GTTCCCCATC CTTTTTGAGC TGTCATGCAT CATCAACAGA 
TGCAAACTGG ACGTCCGGAC CAGGGGTCTG ACAGTGATGT TTGAGGTGAT GAAGACGTAC 
GGTCATACCT ATGAGAAACA CTGGTGGCAG GATCTGTTCC GCATCGTCTT CAGGATCTTC 
GACAACATGA AGCTTCCTGA GCAACAGACC GAGAAAGCGG AGTGGATGAC CACCACCTGC 
AATCATGCAC TTTATGCCAT CTGTGACGTT TTTACACAGT ACTTTGAGTC TCTCAGTGAT 
GTTCTTTTAG ATGATATCTT ATCTCAGCTC TACTGGTGTG TCCAGCAAGA TAATGAGCAA 
TTAGCCAGAT CAGGCACTAA CTGCCTTGAA AACGTGGTCA TCCTTAATGG TGAGAAGTTC 
AATCCTGAGA CTTGGGACAA GACATGCAAC TGCATGCTGG ACATCTTCAA AACCACCATC 
CCACACATGC TGCTGACTTG GAGACCGGCT GGTGCTGAAG GAGATCACAT GACTCAGCTG 
GAGTCTGATA AACAGCTGGA CTCCATTTCC CAGAAGTCAG TGGATATTCA GACCCGCTCA 
GATGACCAGC AGTCTGTTAA CAGTATGGAG AAGGCTTTGG CTGACAACCG CAGATACAGT 
CAGTTCAGTG TGGCTTCAGA AGCTCAGGAA CAGAGGCTGT TTGCTGCGCT ACTCATTAAG 
TGTGTGGTTC AGCTGGAGCT CATTCAGACC ATTGACAACA TCGTCTTCTT TCCTGCTACC 
AGTAAGAAAG AAGATGCGGA AAACTTTGCG GCTGCACAGA GAGATGCTTT AGATGCTGAT 
ATACATGTGG ACACACAGGA CCAGGGAATG TATCGCTATC TGACCTCAGA ACAGCTCTTC 
AAACTGCTCG ACTGTCTGCT GGAGTCTCAT CGATTTGCAA AGGCCTTCAA CTCCAATAAT 
GAGCAAAGAA CTGCTCTGTG GAAAGCCGGT TTCAAAGGGA AGTCGAAGCC CAATCTGCTG 
AAACAGGAGA CGAGCAGCCT GGCGTGTGGT CTGCGCATCC TCTTCCGCAT GTACACAGAT 
CCAAGCCGAC GGGATGCCTG GGAGGAGGTG CAGAGGAGAC TGCTCAACGT TTGCAGTGAC 
GCAGTGGCTT ATTTTCTGAC CCTCACGTCT GAAAGTCATC GCGAAGCCTG GACAAACCTT 
CTGCTGCTCT TCCTGACCAA AGTGCTGAAG ATCAGTGATG ATAGATTCAA GGCTCATGCG 
TCCCGCTACT ACCCTCTGCT CTGTGAAATC ATGCAGTTCG ACCTGATCCC GGAGCTGCGG 
GCCGTCCTGC GCAAGTTTTT CCTGCGCATT GGACTGGTTT TCCAGATCAC CAACAGCCTG 
GAAACAAATC AAACTGTCAG CAACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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arfgef1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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