gRNAs
The following Arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa61200 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017593322.1 | |
| Accession Version | XM_017593322.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017448811.1 |
| CDS | 311..5704 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017593322.1 |
1 | ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11581 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001277056.1 | |
| Accession Version | NM_001277056.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | brefeldin A-inhibited guanine nucleotide-exchange protein 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07046830.1. On or before Mar 2, 2013 this sequence version replaced XM_001066125.3, XM_232614.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001263985.1 |
| CDS | 157..5697 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001277056.1 |
1 | ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange factor, is required for GABA-gated Cl? influx through regulation of GABAA receptor trafficking. |
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein regulates neurite development via PI3K-AKT and ERK signaling pathways. |
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function. |
Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing. |
Specific functions of BIG1 and BIG2 in endomembrane organization. |
