SEC7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC7
Entrez Gene ID	851748
Full Name	Arf family guanine nucleotide exchange factor SEC7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180477.3	NP_010454.3	Arf family guanine nucleotide exchange factor SEC7

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix.
Title: The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.

Sec7 is an effector of the Ypt1 and Ypt31/32 GTPases.
Title: Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network.

Crystallographic, NMR spectrum and mutagenesis studies suggested that the loop>J motif with a key conserved residue Ile1010 modulates the fine conformation of Sec7 domain and thereby regulates its guanine exchange activity.
Title: C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation.

analysis of the C-terminal sequence in the guanine nucleotide exchange factor Sec7 that mediates Golgi association and interaction with the Rsp5 ubiquitin ligase
Title: A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase.

The following SEC7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01252	NM_001180477.3 Latest version!	Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor SEC7 (SEC7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC7 ( NM_001180477.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010454.3 Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor SEC7 (SEC7), partial mRNA.

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CloneID	OSi01252
Clone ID Related Accession (Same CDS sequence)	NM_001180477.3
Accession Version	NM_001180477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Arf family guanine nucleotide exchange factor SEC7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180477.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAAC AGAATTCAGT AGTAAATGCT GAAAAAGGGG ATGGCGAAAT ATCTTCAAAT 
GTAGAGACTG CTTCTTCAGT CAATCCGTCT GTGAAACCAC AGAATGCTAT TAAAGAAGAA 
GCTAAAGAGA CAAATGGTGA AGATCAAAAA TGCAAAGGGC CAGAAAACGC TGGCAGTACG 
GCAGAAACAA AAGAAACAAG TAACGACGCT ACCAACGGAA TGAAAACGCC CGAAGAAACG 
GAAGATACAA ATGACAAACG CCATGACGAT GAAGGTGAAG ATGGAGATGA AGATGAAGAT 
GAAGATGAAG ATGAAGATGA AGACAATGGG GACGAGGATG ATGAGGACGT GGATAGCAGT 
AGTTCCGAAA CCAGTTCCGA AGACGGGGAA GATTCTGAAT CAGTCTCAGG GGAAAGTACT 
GAGAGTAGCT CTGGAGAAGA TGAAGAATCT GATGAAAGCG ATGGAAATAC ATCAAACAGC 
TCCTCTGGTG ATGAAAGCGG GTCAGAAGAA GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA 
GAGGAAAATG CTGGCGAACC AGCTATCGCT CATCAAGATA GTGTTCCCAC CAACGATTCT 
ACTGCCCCAA GGTCTACCCA TACGAGGAAC ATATCACTAT CTTCAAATGG TTCGAACACA 
AACTCAACCA TAATTTTAGT GAAAACTACG TTGGAGACAA TTTTAAATGA TAAGGACATT 
AAAAAGAATT CGAATGCTCA GAAGGCTATC GAAAGGACAT TACAAAAATT TAAGGAATTT 
GATCCGCAAA CCACGAATAA CCCACATTAC GTGGATTCAA TACTAGTATT CGAAGCACTA 
AGGGCAAGCT GTCGTACCAA ATCCTCCAAA GTTCAAAGTT TGGCTTTAGA TTGCCTGTCA 
AAATTGTTTT CTTTTAGATC GCTAGACGAG ACCCTGTTAG TGAATCCACC CGATTCTTTA 
GCCTCTAATG ATCAACGACA AGATGCTGCC GATGGAATAA CGCCGCCTCC AAAACAAAAA 
ATTATAGACG CTGCAATTGA TACTATTTCA GACTGTTTTC AAGGTGAAGG CACTGATGAC 
CGTGTGGAAC TACAAATCGT TAGAGCTTTA TCTAGTTGCA TTTTAGAAGA GGATTCAAGT 
TCTTTATGCC ACGGTGCTTC CTTGCTAAAG GCTATCAGAA CAATCTACAA CGTTTTCGTC 
TTTTCGTTGA ACCCATCCAA TCAAGGTATT GCACAGGCGA CCTTGACACA AATTATTAGT 
TCGGTGTATG ATAAAATCGA TCTCAAACAA AGTACCTCTT CAGCAGTATC CTTATCGACA 
AAAAATCATC AACAACAATC CGCCATAGAA CTTTCCGAGG CTTCTGAAAA TGCAGAAACG 
CCGGCTCCTT TAACTCTGGA AAATATGGAT AAGTTGAATG ACGATGAGGA AAGACTAATG 
GACGCTCAAC AGCCTGATTC TATCGCCATA ACTAACCAAG ATCTAGCTGT TAAAGATGCG 
TTCTTAGTGT TTAGAGTCAT GGCGAAAATA TGTGCTAAAC CTTTGGAAAC AGAACTCGAC 
ATGAGGTCAC ATGCCGTCAG GTCAAAGCTT TTATCTCTTC ACATCATTTA CTCTATTATC 
AAAGATCATA TTGACGTATT CCTTTCCCAC AACATTTTTC TACCAGGAAA GGAGCGTGTG 
TGCTTTATCG ATTCAATTAG ACAATATTTA CGTCTTGTTT TATCAAGGAA TGCTGCCTCG 
CCTCTAGCTC CAGTTTTCGA GGTTACTTTA GAAATTATGT GGCTATTGAT TGCTAATTTG 
AGAGCAGATT TCGTGAAGGA AATTCCAGTT TTTTTAACAG AAATCTACTT CCCCATTTCA 
GAATTAACCA CTTCCACCTC CCAACAAAAG AGATATTTTT TAAGTGTTAT TCAACGAATT 
TGTAACGACC CAAGAACTTT AGTTGAATTT TACTTGAATT ATGATTGTAA CCCTGGAATG 
CCAAATGTAA TGGAAATAAC TGTTGATTAT TTGACAAGAT TGGCTTTAAC TAGGGTGGAA 
ATTACTCAGA CTCAAAGATC ATACTACGAT GAACAAATAT CAAAATCCCT GTCCACCTAC 
AATTTCTCAC AATTGCCCCT TTTGACTTCT TCCAACTTGT CTTCTAGTCC TGATGTTGGT 
CAAGTCAATT TACTTTTTCC ACTTGATTTT GCTCTCAAAA TGGTGTCACT AAATTGCATT 
GTGTCAGTTT TGCGTTCCTT AAGCTCATGG GCTCACAAAG CTTTAAATCC AAACACACAC 
ACTGCTAATA AGGTATTACT AAACACAACA TCTTCAGCTC GTCAAGAATC TAGATCATCT 
TTGAGTAACG ACGTAAGGTC TTCTATTATG ACAAGTAATG ATGACTTCAA ACCAACATAT 
GAAGACGAAG AAAGCAGATC ATTGAGCAGT CAAAACATTG ACGCAGACGA CCCCACACAA 
TTTGAAAATT TGAAACTAAG GAAAACTGCT TTATCGGAAT GTATTGCTAT TTTCAACAAT 
AAACCCAAGA AAGCTATTCC AGTACTTATT AAAAAAGGAT TTTTGAAAGA TGATTCTCCA 
ATTTCCATTG CCAAGTGGTT ATTAGAAACA GAAGGCCTCG ACATGGCCGC TGTTGGGGAT 
TATCTAGGCG AAGGAGACGA CAAGAACATC GCTATAATGC ACGCATTTGT TGATGAGTTT 
GACTTCACTG GTATGTCCAT TGTTGACGCA TTAAGGTCAT TTTTACAAAG TTTCAGATTG 
CCTGGAGAAG GTCAAAAAAT TGACAGATTC ATGCTGAAAT TTGCGGAAAG ATTTGTGGAC 
CAAAACCCCG GAGTCTTTTC AAAGGCGGAT ACTGCATATG TGCTTTCGTA TTCTTTGATC 
ATGTTGAATA CTGATTTACA TTCGTCACAA ATCAAAAATA AAATGTCTTT ACAAGAGTTT 
TTAGAAAACA ACGAAGGTAT TGACAACGGA AGAGATTTAC CAAGAGACTT CTTGGAAGGT 
TTGTTCAACG AAATTGCTAA CAATGAAATC AAGTTAATTT CTGAACAGCA TCAGGCAATG 
CTTTCAGGTG ATACCAATCT TGTCCAACAA CAGCAATCTG CTTTCAACTT CTTTAATTCT 
CGTGATTTGA CAAGAGAGGC ATATAATCAA GTCTCAAAAG AAATTTCATC TAAAACGGAA 
TTAGTCTTTA AGAATTTAAA CAAAAATAAA GGAGGCCCAG ACGTCTATTA TGCTGCTTCC 
CACGTTGAGC ATGTTAAATC AATTTTCGAG ACACTATGGA TGTCCTTTTT AGCAGCTCTA 
ACCCCCCCAT TTAAGGATTA TGATGACATT GACACAACCA ATAAGTGTTT AGAAGGCTTG 
AAAATATCAA TTAAAATTGC TTCTACTTTT AGAATCAATG ATGCTAGAAC CTCCTTTGTA 
GGTGCGTTAG TCCAATTTTG TAACCTTCAA AACCTTGAAG AAATCAAAGT TAAAAATGTC 
AATGCAATGG TAATTCTTCT TGAAGTCGCG TTATCAGAAG GAAATTACTT GGAGGGATCG 
TGGAAGGACA TTTTGCTGGT CGTGTCTCAA ATGGAAAGAC TACAATTGAT ATCCAAAGGT 
ATCGACAGAG ATACGGTTCC AGATGTTGCA CAAGCTCGTG TTGCAAACCC TAGAGTTTCT 
TACGAATCAT CAAGATCCAA TAATACATCT TTCTTTGATG TTTGGGGCAA GAAGGCAACT 
CCCACAGAAT TGGCTCAAGA AAAACACCAT AATCAAACCT TATCACCCGA AATCTCTAAA 
TTCATTTCCT CCAGTGAATT AGTCGTTTTG ATGGACAATA TATTTACCAA AAGTTCCGAG 
TTATCAGGTA ATGCTATCGT TGATTTTATC AAAGCTTTAA CCGCTGTATC TTTAGAAGAA 
ATTGAATCAT CTGAAAATGC TTCCACACCA AGAATGTTTT CCTTGCAAAA AATGGTCGAT 
GTATGTTACT ACAATATGGA TCGTATCAAA CTAGAATGGA CGCCGCTTTG GGCTGTTATG 
GGAAAAGCTT TCAACAAGAT TGCTACAAAC TCTAACTTAG CAGTAGTATT TTTCGCTATC 
GATTCCCTGC GTCAATTGTC TATGAGATTT TTAGATATTG AGGAATTATC AGGTTTTGAA 
TTTCAACATG ATTTTTTAAA ACCTTTTGAA TACACGGTTC AAAATAGTGG CAACACTGAA 
GTTCAAGAAA TGATTATTGA GTGTTTTAGG AACTTCATAT TGACAAAATC GGAAAGTATC 
AAATCTGGCT GGAAGCCTAT TCTTGAATCC TTACAATATA CGGCTCGCTC AAGCACTGAA 
TCCATTGTAT TAAAGACGCA ATTGCTGGTT AGCAATGATA TTGTGACAAA TCACTTCGAA 
AACGTATTCT CTCAAGAAGA TGCCTTTTCT GAGTTAGTTG GTGTCTTCAG AGAAATCACC 
AAAAATAAGA GATTCCAAAA GCTATCTCTA CATGCTTTGG AGTCTCTAAG AAAGATGACT 
CAGAACGTCG CAGACATCTG TTTTTACAAT GAAAATAAGA CTGAAGAAGA AAGAAAACAT 
AACGATGCTT TACTTCGTGG GAAAGACATA TTCCAGGATG TGTGGTTCCC TATGTTATTC 
TGCTTCAATG ATACGATCAT GACAGCTGAA GACTTAGAAG TTAGATCACG TGCATTAAAC 
TATATGTTCG ATGCCCTAGT GGCATATGGT GGTAAATTCA ATGATGATTT CTGGGAAAAG 
ATTTGTAAGA AGTTACTATT TCCTATTTTC GGTGTATTAT CCAAACATTG GGAAGTCAAC 
CAATTCAATA GTCATGATGA TTTAAGTGTT TGGCTTTCGA CCACGTTAAT TCAAGCCTTA 
AGGAACTTGA TTGCCCTTTT TACGCATTAC TTTGAATCGT TGAACAGAAT GCTCGATGGA 
TTTTTGGGTC TGCTGGTATC CTGTATTTGT CAAGAAAATG ACACTATTGC TAGAATTGGG 
AGATCCTGCC TGCAACAATT GATATTGCAA AACGTATCTA AATTCAATGA GTACCATTGG 
AATCAAATAG GTGACGTATT CGATAAATTG TTTGATTTAA CCACTGCTAA TGAATTGTTC 
GATTATGATC CGTTACAGCA AGGAAGAAAA TCATCTGTAT CTCATCACCA AACAACTAAT 
GATACCAGCC AACACTCAGA TGATGATAGC AACGACAGAA GGGAGAACGA TTCTAATATC 
AGTGAGACGG TCGAAAGAGC GCATCAAGAA GAATCCAGCG AAGACGTTGG TGGTGACATG 
GTTGAAACAC TCAATGGGCA AACTAAGCTT AATAATGGCA ATTCCGTTCC GACGGTAAAG 
GACGAATTGA ATCCAAAGCC CGCTAGTCTA AGTATCCCCA AGAAAACTAA GCACATGAAA 
CGAAACGAAA GTAACGAAGA TATACGTAGG AGAATAAACA TCAAGAATTC TATTGTTGTC 
AAATGTGTTT TACAGCTTTT GATGATAGAG CTGCTCAACG AATTATTCGA GAACGAAGAT 
TTTGCCCACT GTATCCCTTA CAAGGAAGCC ATTAGAATTA CAAGATTGTT GGAGAAATCG 
TACGAATTTT CTCGTGACTT TAATGAAGAT TATGGGTTAA GAACAAGACT AGTAGAGGCT 
CGTGTAGTTG ATAAAATACC AAACCTGTTG AAACAAGAAA CGAGTGCTGC TGCAGTTCTC 
CTTGACATTA TGTTTCAGTT GTATCTGAAC GATGATGAGA AGAAGGCTGA TTTAATAACT 
CGTCTGATCA CCATTTGCAT CCAGGTCGTA GAGGGTTATG TTTCTTTGGA TGATAGAACC 
ATGGAACGTA GTATCAATGC CTGGCGTTCA GTGATAGTTG AAATATTACA AGGTTACTAC 
GAATTTGATG ATGAAGACTT TAGACTATAC TGTCCTGCTA TGTATGCTCT GGTGATTCAA 
ATATTAGATA AGAGCGTTCC AACGGAGTTG AGACATGCTA TAAAACAATT TCTAAGCAGA 
GTTGGTGAAT TATACCTTTC TACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010454.3
CDS	1..6030
Translation	MSEQNSVVNAEKGDGEISSNVETASSVNPSVKPQNAIKEEAKETNGEDQKCKGPENAGSTAETKETSNDATNGMKTPEETEDTNDKRHDDEGEDGDEDEDEDEDEDEDNGDEDDEDVDSSSSETSSEDGEDSESVSGESTESSSGEDEESDESDGNTSNSSSGDESGSEEEEEEEEEEEEEENAGEPAIAHQDSVPTNDSTAPRSTHTRNISLSSNGSNTNSTIILVKTTLETILNDKDIKKNSNAQKAIERTLQKFKEFDPQTTNNPHYVDSILVFEALRASCRTKSSKVQSLALDCLSKLFSFRSLDETLLVNPPDSLASNDQRQDAADGITPPPKQKIIDAAIDTISDCFQGEGTDDRVELQIVRALSSCILEEDSSSLCHGASLLKAIRTIYNVFVFSLNPSNQGIAQATLTQIISSVYDKIDLKQSTSSAVSLSTKNHQQQSAIELSEASENAETPAPLTLENMDKLNDDEERLMDAQQPDSIAITNQDLAVKDAFLVFRVMAKICAKPLETELDMRSHAVRSKLLSLHIIYSIIKDHIDVFLSHNIFLPGKERVCFIDSIRQYLRLVLSRNAASPLAPVFEVTLEIMWLLIANLRADFVKEIPVFLTEIYFPISELTTSTSQQKRYFLSVIQRICNDPRTLVEFYLNYDCNPGMPNVMEITVDYLTRLALTRVEITQTQRSYYDEQISKSLSTYNFSQLPLLTSSNLSSSPDVGQVNLLFPLDFALKMVSLNCIVSVLRSLSSWAHKALNPNTHTANKVLLNTTSSARQESRSSLSNDVRSSIMTSNDDFKPTYEDEESRSLSSQNIDADDPTQFENLKLRKTALSECIAIFNNKPKKAIPVLIKKGFLKDDSPISIAKWLLETEGLDMAAVGDYLGEGDDKNIAIMHAFVDEFDFTGMSIVDALRSFLQSFRLPGEGQKIDRFMLKFAERFVDQNPGVFSKADTAYVLSYSLIMLNTDLHSSQIKNKMSLQEFLENNEGIDNGRDLPRDFLEGLFNEIANNEIKLISEQHQAMLSGDTNLVQQQQSAFNFFNSRDLTREAYNQVSKEISSKTELVFKNLNKNKGGPDVYYAASHVEHVKSIFETLWMSFLAALTPPFKDYDDIDTTNKCLEGLKISIKIASTFRINDARTSFVGALVQFCNLQNLEEIKVKNVNAMVILLEVALSEGNYLEGSWKDILLVVSQMERLQLISKGIDRDTVPDVAQARVANPRVSYESSRSNNTSFFDVWGKKATPTELAQEKHHNQTLSPEISKFISSSELVVLMDNIFTKSSELSGNAIVDFIKALTAVSLEEIESSENASTPRMFSLQKMVDVCYYNMDRIKLEWTPLWAVMGKAFNKIATNSNLAVVFFAIDSLRQLSMRFLDIEELSGFEFQHDFLKPFEYTVQNSGNTEVQEMIIECFRNFILTKSESIKSGWKPILESLQYTARSSTESIVLKTQLLVSNDIVTNHFENVFSQEDAFSELVGVFREITKNKRFQKLSLHALESLRKMTQNVADICFYNENKTEEERKHNDALLRGKDIFQDVWFPMLFCFNDTIMTAEDLEVRSRALNYMFDALVAYGGKFNDDFWEKICKKLLFPIFGVLSKHWEVNQFNSHDDLSVWLSTTLIQALRNLIALFTHYFESLNRMLDGFLGLLVSCICQENDTIARIGRSCLQQLILQNVSKFNEYHWNQIGDVFDKLFDLTTANELFDYDPLQQGRKSSVSHHQTTNDTSQHSDDDSNDRRENDSNISETVERAHQEESSEDVGGDMVETLNGQTKLNNGNSVPTVKDELNPKPASLSIPKKTKHMKRNESNEDIRRRINIKNSIVVKCVLQLLMIELLNELFENEDFAHCIPYKEAIRITRLLEKSYEFSRDFNEDYGLRTRLVEARVVDKIPNLLKQETSAAAVLLDIMFQLYLNDDEKKADLITRLITICIQVVEGYVSLDDRTMERSINAWRSVIVEILQGYYEFDDEDFRLYCPAMYALVIQILDKSVPTELRHAIKQFLSRVGELYLSTD

Target ORF information:

RefSeq Version	NM_001180477.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor SEC7 (SEC7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180477.3

ORF Insert Sequence:

ATGTCTGAAC AGAATTCAGT AGTAAATGCT GAAAAAGGGG ATGGCGAAAT ATCTTCAAAT 
GTAGAGACTG CTTCTTCAGT CAATCCGTCT GTGAAACCAC AGAATGCTAT TAAAGAAGAA 
GCTAAAGAGA CAAATGGTGA AGATCAAAAA TGCAAAGGGC CAGAAAACGC TGGCAGTACG 
GCAGAAACAA AAGAAACAAG TAACGACGCT ACCAACGGAA TGAAAACGCC CGAAGAAACG 
GAAGATACAA ATGACAAACG CCATGACGAT GAAGGTGAAG ATGGAGATGA AGATGAAGAT 
GAAGATGAAG ATGAAGATGA AGACAATGGG GACGAGGATG ATGAGGACGT GGATAGCAGT 
AGTTCCGAAA CCAGTTCCGA AGACGGGGAA GATTCTGAAT CAGTCTCAGG GGAAAGTACT 
GAGAGTAGCT CTGGAGAAGA TGAAGAATCT GATGAAAGCG ATGGAAATAC ATCAAACAGC 
TCCTCTGGTG ATGAAAGCGG GTCAGAAGAA GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA 
GAGGAAAATG CTGGCGAACC AGCTATCGCT CATCAAGATA GTGTTCCCAC CAACGATTCT 
ACTGCCCCAA GGTCTACCCA TACGAGGAAC ATATCACTAT CTTCAAATGG TTCGAACACA 
AACTCAACCA TAATTTTAGT GAAAACTACG TTGGAGACAA TTTTAAATGA TAAGGACATT 
AAAAAGAATT CGAATGCTCA GAAGGCTATC GAAAGGACAT TACAAAAATT TAAGGAATTT 
GATCCGCAAA CCACGAATAA CCCACATTAC GTGGATTCAA TACTAGTATT CGAAGCACTA 
AGGGCAAGCT GTCGTACCAA ATCCTCCAAA GTTCAAAGTT TGGCTTTAGA TTGCCTGTCA 
AAATTGTTTT CTTTTAGATC GCTAGACGAG ACCCTGTTAG TGAATCCACC CGATTCTTTA 
GCCTCTAATG ATCAACGACA AGATGCTGCC GATGGAATAA CGCCGCCTCC AAAACAAAAA 
ATTATAGACG CTGCAATTGA TACTATTTCA GACTGTTTTC AAGGTGAAGG CACTGATGAC 
CGTGTGGAAC TACAAATCGT TAGAGCTTTA TCTAGTTGCA TTTTAGAAGA GGATTCAAGT 
TCTTTATGCC ACGGTGCTTC CTTGCTAAAG GCTATCAGAA CAATCTACAA CGTTTTCGTC 
TTTTCGTTGA ACCCATCCAA TCAAGGTATT GCACAGGCGA CCTTGACACA AATTATTAGT 
TCGGTGTATG ATAAAATCGA TCTCAAACAA AGTACCTCTT CAGCAGTATC CTTATCGACA 
AAAAATCATC AACAACAATC CGCCATAGAA CTTTCCGAGG CTTCTGAAAA TGCAGAAACG 
CCGGCTCCTT TAACTCTGGA AAATATGGAT AAGTTGAATG ACGATGAGGA AAGACTAATG 
GACGCTCAAC AGCCTGATTC TATCGCCATA ACTAACCAAG ATCTAGCTGT TAAAGATGCG 
TTCTTAGTGT TTAGAGTCAT GGCGAAAATA TGTGCTAAAC CTTTGGAAAC AGAACTCGAC 
ATGAGGTCAC ATGCCGTCAG GTCAAAGCTT TTATCTCTTC ACATCATTTA CTCTATTATC 
AAAGATCATA TTGACGTATT CCTTTCCCAC AACATTTTTC TACCAGGAAA GGAGCGTGTG 
TGCTTTATCG ATTCAATTAG ACAATATTTA CGTCTTGTTT TATCAAGGAA TGCTGCCTCG 
CCTCTAGCTC CAGTTTTCGA GGTTACTTTA GAAATTATGT GGCTATTGAT TGCTAATTTG 
AGAGCAGATT TCGTGAAGGA AATTCCAGTT TTTTTAACAG AAATCTACTT CCCCATTTCA 
GAATTAACCA CTTCCACCTC CCAACAAAAG AGATATTTTT TAAGTGTTAT TCAACGAATT 
TGTAACGACC CAAGAACTTT AGTTGAATTT TACTTGAATT ATGATTGTAA CCCTGGAATG 
CCAAATGTAA TGGAAATAAC TGTTGATTAT TTGACAAGAT TGGCTTTAAC TAGGGTGGAA 
ATTACTCAGA CTCAAAGATC ATACTACGAT GAACAAATAT CAAAATCCCT GTCCACCTAC 
AATTTCTCAC AATTGCCCCT TTTGACTTCT TCCAACTTGT CTTCTAGTCC TGATGTTGGT 
CAAGTCAATT TACTTTTTCC ACTTGATTTT GCTCTCAAAA TGGTGTCACT AAATTGCATT 
GTGTCAGTTT TGCGTTCCTT AAGCTCATGG GCTCACAAAG CTTTAAATCC AAACACACAC 
ACTGCTAATA AGGTATTACT AAACACAACA TCTTCAGCTC GTCAAGAATC TAGATCATCT 
TTGAGTAACG ACGTAAGGTC TTCTATTATG ACAAGTAATG ATGACTTCAA ACCAACATAT 
GAAGACGAAG AAAGCAGATC ATTGAGCAGT CAAAACATTG ACGCAGACGA CCCCACACAA 
TTTGAAAATT TGAAACTAAG GAAAACTGCT TTATCGGAAT GTATTGCTAT TTTCAACAAT 
AAACCCAAGA AAGCTATTCC AGTACTTATT AAAAAAGGAT TTTTGAAAGA TGATTCTCCA 
ATTTCCATTG CCAAGTGGTT ATTAGAAACA GAAGGCCTCG ACATGGCCGC TGTTGGGGAT 
TATCTAGGCG AAGGAGACGA CAAGAACATC GCTATAATGC ACGCATTTGT TGATGAGTTT 
GACTTCACTG GTATGTCCAT TGTTGACGCA TTAAGGTCAT TTTTACAAAG TTTCAGATTG 
CCTGGAGAAG GTCAAAAAAT TGACAGATTC ATGCTGAAAT TTGCGGAAAG ATTTGTGGAC 
CAAAACCCCG GAGTCTTTTC AAAGGCGGAT ACTGCATATG TGCTTTCGTA TTCTTTGATC 
ATGTTGAATA CTGATTTACA TTCGTCACAA ATCAAAAATA AAATGTCTTT ACAAGAGTTT 
TTAGAAAACA ACGAAGGTAT TGACAACGGA AGAGATTTAC CAAGAGACTT CTTGGAAGGT 
TTGTTCAACG AAATTGCTAA CAATGAAATC AAGTTAATTT CTGAACAGCA TCAGGCAATG 
CTTTCAGGTG ATACCAATCT TGTCCAACAA CAGCAATCTG CTTTCAACTT CTTTAATTCT 
CGTGATTTGA CAAGAGAGGC ATATAATCAA GTCTCAAAAG AAATTTCATC TAAAACGGAA 
TTAGTCTTTA AGAATTTAAA CAAAAATAAA GGAGGCCCAG ACGTCTATTA TGCTGCTTCC 
CACGTTGAGC ATGTTAAATC AATTTTCGAG ACACTATGGA TGTCCTTTTT AGCAGCTCTA 
ACCCCCCCAT TTAAGGATTA TGATGACATT GACACAACCA ATAAGTGTTT AGAAGGCTTG 
AAAATATCAA TTAAAATTGC TTCTACTTTT AGAATCAATG ATGCTAGAAC CTCCTTTGTA 
GGTGCGTTAG TCCAATTTTG TAACCTTCAA AACCTTGAAG AAATCAAAGT TAAAAATGTC 
AATGCAATGG TAATTCTTCT TGAAGTCGCG TTATCAGAAG GAAATTACTT GGAGGGATCG 
TGGAAGGACA TTTTGCTGGT CGTGTCTCAA ATGGAAAGAC TACAATTGAT ATCCAAAGGT 
ATCGACAGAG ATACGGTTCC AGATGTTGCA CAAGCTCGTG TTGCAAACCC TAGAGTTTCT 
TACGAATCAT CAAGATCCAA TAATACATCT TTCTTTGATG TTTGGGGCAA GAAGGCAACT 
CCCACAGAAT TGGCTCAAGA AAAACACCAT AATCAAACCT TATCACCCGA AATCTCTAAA 
TTCATTTCCT CCAGTGAATT AGTCGTTTTG ATGGACAATA TATTTACCAA AAGTTCCGAG 
TTATCAGGTA ATGCTATCGT TGATTTTATC AAAGCTTTAA CCGCTGTATC TTTAGAAGAA 
ATTGAATCAT CTGAAAATGC TTCCACACCA AGAATGTTTT CCTTGCAAAA AATGGTCGAT 
GTATGTTACT ACAATATGGA TCGTATCAAA CTAGAATGGA CGCCGCTTTG GGCTGTTATG 
GGAAAAGCTT TCAACAAGAT TGCTACAAAC TCTAACTTAG CAGTAGTATT TTTCGCTATC 
GATTCCCTGC GTCAATTGTC TATGAGATTT TTAGATATTG AGGAATTATC AGGTTTTGAA 
TTTCAACATG ATTTTTTAAA ACCTTTTGAA TACACGGTTC AAAATAGTGG CAACACTGAA 
GTTCAAGAAA TGATTATTGA GTGTTTTAGG AACTTCATAT TGACAAAATC GGAAAGTATC 
AAATCTGGCT GGAAGCCTAT TCTTGAATCC TTACAATATA CGGCTCGCTC AAGCACTGAA 
TCCATTGTAT TAAAGACGCA ATTGCTGGTT AGCAATGATA TTGTGACAAA TCACTTCGAA 
AACGTATTCT CTCAAGAAGA TGCCTTTTCT GAGTTAGTTG GTGTCTTCAG AGAAATCACC 
AAAAATAAGA GATTCCAAAA GCTATCTCTA CATGCTTTGG AGTCTCTAAG AAAGATGACT 
CAGAACGTCG CAGACATCTG TTTTTACAAT GAAAATAAGA CTGAAGAAGA AAGAAAACAT 
AACGATGCTT TACTTCGTGG GAAAGACATA TTCCAGGATG TGTGGTTCCC TATGTTATTC 
TGCTTCAATG ATACGATCAT GACAGCTGAA GACTTAGAAG TTAGATCACG TGCATTAAAC 
TATATGTTCG ATGCCCTAGT GGCATATGGT GGTAAATTCA ATGATGATTT CTGGGAAAAG 
ATTTGTAAGA AGTTACTATT TCCTATTTTC GGTGTATTAT CCAAACATTG GGAAGTCAAC 
CAATTCAATA GTCATGATGA TTTAAGTGTT TGGCTTTCGA CCACGTTAAT TCAAGCCTTA 
AGGAACTTGA TTGCCCTTTT TACGCATTAC TTTGAATCGT TGAACAGAAT GCTCGATGGA 
TTTTTGGGTC TGCTGGTATC CTGTATTTGT CAAGAAAATG ACACTATTGC TAGAATTGGG 
AGATCCTGCC TGCAACAATT GATATTGCAA AACGTATCTA AATTCAATGA GTACCATTGG 
AATCAAATAG GTGACGTATT CGATAAATTG TTTGATTTAA CCACTGCTAA TGAATTGTTC 
GATTATGATC CGTTACAGCA AGGAAGAAAA TCATCTGTAT CTCATCACCA AACAACTAAT 
GATACCAGCC AACACTCAGA TGATGATAGC AACGACAGAA GGGAGAACGA TTCTAATATC 
AGTGAGACGG TCGAAAGAGC GCATCAAGAA GAATCCAGCG AAGACGTTGG TGGTGACATG 
GTTGAAACAC TCAATGGGCA AACTAAGCTT AATAATGGCA ATTCCGTTCC GACGGTAAAG 
GACGAATTGA ATCCAAAGCC CGCTAGTCTA AGTATCCCCA AGAAAACTAA GCACATGAAA 
CGAAACGAAA GTAACGAAGA TATACGTAGG AGAATAAACA TCAAGAATTC TATTGTTGTC 
AAATGTGTTT TACAGCTTTT GATGATAGAG CTGCTCAACG AATTATTCGA GAACGAAGAT 
TTTGCCCACT GTATCCCTTA CAAGGAAGCC ATTAGAATTA CAAGATTGTT GGAGAAATCG 
TACGAATTTT CTCGTGACTT TAATGAAGAT TATGGGTTAA GAACAAGACT AGTAGAGGCT 
CGTGTAGTTG ATAAAATACC AAACCTGTTG AAACAAGAAA CGAGTGCTGC TGCAGTTCTC 
CTTGACATTA TGTTTCAGTT GTATCTGAAC GATGATGAGA AGAAGGCTGA TTTAATAACT 
CGTCTGATCA CCATTTGCAT CCAGGTCGTA GAGGGTTATG TTTCTTTGGA TGATAGAACC 
ATGGAACGTA GTATCAATGC CTGGCGTTCA GTGATAGTTG AAATATTACA AGGTTACTAC 
GAATTTGATG ATGAAGACTT TAGACTATAC TGTCCTGCTA TGTATGCTCT GGTGATTCAA 
ATATTAGATA AGAGCGTTCC AACGGAGTTG AGACATGCTA TAAAACAATT TCTAAGCAGA 
GTTGGTGAAT TATACCTTTC TACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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