RAPGEF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF1
Entrez Gene ID	520454
Full Name	Rap guanine nucleotide exchange factor 1
General protein information	Preferred Names Rap guanine nucleotide exchange factor 1 Names rap guanine nucleotide exchange factor 1 Rap guanine nucleotide exchange factor (GEF) 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473626.1	XP_015329112.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_010810421.2	XP_010808723.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015473631.1	XP_015329117.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015473633.1	XP_015329119.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_015473638.1	XP_015329124.1	rap guanine nucleotide exchange factor 1 isoform X14
XM_024998674.1	XP_024854442.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_024998675.1	XP_024854443.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_024998679.1	XP_024854447.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_024998680.1	XP_024854448.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_024998690.1	XP_024854458.1	rap guanine nucleotide exchange factor 1 isoform X13
XM_024998693.1	XP_024854461.1	rap guanine nucleotide exchange factor 1 isoform X16
XM_015473638.2	XP_015329124.1	rap guanine nucleotide exchange factor 1 isoform X19
XM_024998681.1	XP_024854449.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_015473625.2	XP_015329111.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_024998689.1	XP_024854457.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_015473634.2	XP_015329120.1	rap guanine nucleotide exchange factor 1 isoform X14
XM_024998696.1	XP_024854464.1	rap guanine nucleotide exchange factor 1 isoform X21
XM_015473628.1	XP_015329114.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_015473629.1	XP_015329115.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015473630.1	XP_015329116.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015473634.1	XP_015329120.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_015473637.1	XP_015329123.1	rap guanine nucleotide exchange factor 1 isoform X13
XM_024998673.1	XP_024854441.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_024998676.1	XP_024854444.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_024998678.1	XP_024854446.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_024998685.1	XP_024854453.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_024998686.1	XP_024854454.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_015473628.2	XP_015329114.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_024998694.1	XP_024854462.1	rap guanine nucleotide exchange factor 1 isoform X17
XM_024998695.1	XP_024854463.1	rap guanine nucleotide exchange factor 1 isoform X18
XM_024998697.1	XP_024854465.1	rap guanine nucleotide exchange factor 1 isoform X22
XM_010810419.1	XP_010808721.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_015473632.1	XP_015329118.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_015473635.1	XP_015329121.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_015473636.1	XP_015329122.1	rap guanine nucleotide exchange factor 1 isoform X12
NM_001205872.1	NP_001192801.1	rap guanine nucleotide exchange factor 1
XM_024998677.1	XP_024854445.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_024998682.1	XP_024854450.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_024998687.1	XP_024854455.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_024998688.1	XP_024854456.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_024998698.1	XP_024854466.1	rap guanine nucleotide exchange factor 1 isoform X23
XM_015473625.1	XP_015329111.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_005213261.3	XP_005213318.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_015473627.1	XP_015329113.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_015473639.1	XP_015329125.1	rap guanine nucleotide exchange factor 1 isoform X15
XM_015473640.1	XP_015329126.1	rap guanine nucleotide exchange factor 1 isoform X16
XM_015473632.2	XP_015329118.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_024998692.1	XP_024854460.1	rap guanine nucleotide exchange factor 1 isoform X15
XM_015473639.2	XP_015329125.1	rap guanine nucleotide exchange factor 1 isoform X20

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RAPGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220350	XM_024998673.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220351	XM_024998674.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220352	XM_024998675.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220353	XM_024998676.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220354	XM_024998677.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998678.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998686.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998681.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998682.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998680.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998679.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220355	XM_024998685.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220356	XM_024998687.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220357	XM_024998688.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220358	XM_024998689.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220359	XM_024998690.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220360	XM_024998692.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220361	XM_024998693.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220362	XM_024998694.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220363	XM_024998695.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220364	XM_024998696.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220365	XM_024998697.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220366	XM_024998698.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa91979	XM_015473625.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91980	XM_015473626.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91981	XM_015473627.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91982	XM_015473628.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91983	XM_015473632.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91984	XM_015473633.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91985	XM_015473634.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91986	XM_015473635.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91987	XM_015473636.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91988	XM_015473637.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91989	XM_015473638.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91990	XM_015473639.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91991	XM_015473640.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa40953	NM_001205872.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa24398	XM_005213261.3 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa50253	XM_010810419.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa50254	XM_015473631.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa50254	XM_015473630.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa50254	XM_015473629.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa50254	XM_010810421.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OBa91989	XM_015473638.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa91985	XM_015473634.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91982	XM_015473628.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91979	XM_015473625.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91983	XM_015473632.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91990	XM_015473639.1	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF1 ( XM_024998673.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854441.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220350
Clone ID Related Accession (Same CDS sequence)	XM_024998673.1
Accession Version	XM_024998673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATATC CTTGCCGCCT CACCCTCCTT 
CACAGAGCCG TCTGCAGGGT GGGGTCAGCG ATGAGGGGCA GTGCCCAGCC CAGCTCTGGT 
TCACTCAGGA AGGGAGGAGG AGGAGGAGGA GGGCGGCCTT CCCTTCACGT CCCCACCCCT 
GCCCGCGAGG ACGGCTGTTT CTTTAACTCG GGCTCCACAT ACGAGAAGTT CTCACCTTTC 
GCCGACACAT TCAAGAAGCG TGTGAGCAAG AACACGTTCT TCGTGCTGGT GCGGGTGGTG 
GACGAGCTCT GCCTGGTAGA GCTGACAGAA GAGATCCTGA AGCTGCTGAT GGAGCTGGTC 
TTCCGCCTGG TGCGCAGCGG GGAGCTCAGC CTAGCACGCG TGCTCCGCAA GAACATTCTG 
GACAAGGTCG ACCAGAAGAA GCTGCTCCGG TGCGCCAACT CTGACCAGCC CCTGGCGGCC 
CGGGGAGTGG CAGCCAGGCC CGGGACCTTG CACGACTTCC ACAGCCATGA AATAGCCGAG 
CAGCTGACTC TGCTAGATGC CGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT 
TGGGCTAAAG AGCAGAACGA GGAGAAGAGC CCGAACCTGA CCCAGTTCAC GGAGCACTTC 
AACAACATGT CCTACTGGGT CCGGTCGATA ATCATGTTGC AGGAAAAGGC CCAAGACAGG 
GAACGGCTGC TCTTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTC 
AACTCCTACT TGGCCATCCT CTCGGCGCTG GACTCGGCCC CCATTCGCAG GCTGGAGTGG 
CAGAAACAGA CCTCAGAGGG CCTGGCCGAG TACTGCACGC TGATCGACAG CTCGTCATCC 
TTCCGCGCCT ACCGGGCCGC CCTCTCGGAG GTAGAACCGC CCTGCATCCC ATACCTGGGG 
CTCATCCTGC AGGACCTCAC CTTCGTCCAC CTGGGGAACC CAGACTATAT TGACGGGAAG 
GTGAACTTCT CCAAACGCTG GCAGCAGTTC AACATCCTGG ACAGCATGCG GTGCTTCCAG 
CAGGCGCACT ACGACATCCG GAGAAATGAC GACATCATTA ACTTCTTCAA CGACTTCAGT 
GACCACCTGG CCGAGGAGGC CCTATGGGAA CTCTCTCTGA AAATCAAACC CAGGAACATA 
ACGAGGAGGA AGACAGATAG AGAAGAAAAG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854441.1
CDS	129..4124
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998673.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998673.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATATC CTTGCCGCCT CACCCTCCTT 
CACAGAGCCG TCTGCAGGGT GGGGTCAGCG ATGAGGGGCA GTGCCCAGCC CAGCTCTGGT 
TCACTCAGGA AGGGAGGAGG AGGAGGAGGA GGGCGGCCTT CCCTTCACGT CCCCACCCCT 
GCCCGCGAGG ACGGCTGTTT CTTTAACTCG GGCTCCACAT ACGAGAAGTT CTCACCTTTC 
GCCGACACAT TCAAGAAGCG TGTGAGCAAG AACACGTTCT TCGTGCTGGT GCGGGTGGTG 
GACGAGCTCT GCCTGGTAGA GCTGACAGAA GAGATCCTGA AGCTGCTGAT GGAGCTGGTC 
TTCCGCCTGG TGCGCAGCGG GGAGCTCAGC CTAGCACGCG TGCTCCGCAA GAACATTCTG 
GACAAGGTCG ACCAGAAGAA GCTGCTCCGG TGCGCCAACT CTGACCAGCC CCTGGCGGCC 
CGGGGAGTGG CAGCCAGGCC CGGGACCTTG CACGACTTCC ACAGCCATGA AATAGCCGAG 
CAGCTGACTC TGCTAGATGC CGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT 
TGGGCTAAAG AGCAGAACGA GGAGAAGAGC CCGAACCTGA CCCAGTTCAC GGAGCACTTC 
AACAACATGT CCTACTGGGT CCGGTCGATA ATCATGTTGC AGGAAAAGGC CCAAGACAGG 
GAACGGCTGC TCTTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTC 
AACTCCTACT TGGCCATCCT CTCGGCGCTG GACTCGGCCC CCATTCGCAG GCTGGAGTGG 
CAGAAACAGA CCTCAGAGGG CCTGGCCGAG TACTGCACGC TGATCGACAG CTCGTCATCC 
TTCCGCGCCT ACCGGGCCGC CCTCTCGGAG GTAGAACCGC CCTGCATCCC ATACCTGGGG 
CTCATCCTGC AGGACCTCAC CTTCGTCCAC CTGGGGAACC CAGACTATAT TGACGGGAAG 
GTGAACTTCT CCAAACGCTG GCAGCAGTTC AACATCCTGG ACAGCATGCG GTGCTTCCAG 
CAGGCGCACT ACGACATCCG GAGAAATGAC GACATCATTA ACTTCTTCAA CGACTTCAGT 
GACCACCTGG CCGAGGAGGC CCTATGGGAA CTCTCTCTGA AAATCAAACC CAGGAACATA 
ACGAGGAGGA AGACAGATAG AGAAGAAAAG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998674.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854442.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

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CloneID	OBa220351
Clone ID Related Accession (Same CDS sequence)	XM_024998674.1
Accession Version	XM_024998674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCATC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCACGGCA GCTTACCTGT GCCCTCGTAC AAGTCCGTGT TTAGGTCCTA CTCGCAGGAT 
TTCGTGCCTC ACAGCCAAGC TTCCGTCCAG CCTTTCCTTC CGTCTACCTC CTCTTCCTCT 
CCACACTTCC CACCTGTCCA CCAGTATCAG AGCTCTGACT TAGCCGTGCC GCCCGTGGCC 
AGTCTGCCTC CCAGCACCCT GGACGGGCCT CTCTCATCTT CTCAGGAGAG CAGCTTTCAC 
GGGAACACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGCCCCA GACGCCTTCG 
AGTGAGCACG CCTGTGAGGG GGACTACGTC AGTCTGTATT GCTCTGGCCA GAGCAGCGAG 
GAGCTGGCCC GCCCTCGAGG AGAGTCACCC GCGGCGAAGG ATGGACACTC CAGAGACGCC 
ACATCGGCCA GCTGTGTGCC CGGGAAGGAG GGCAGGGACA GCGGGGACAG GGCCCGGAAG 
TCACCAGACG CTCCAGAGGC TCAGTCAGAG GAGGAGGTGG ACGAGCTCTC CCTCATCGAC 
CACAACGAGA TCATGGCGAG GCTGACCCTC AAGCAAGAGG GCGATGACGG GCCAGACGTC 
CGTGGAGGGT CGGGCGACAT CCTCCTCGTC CATGCCACGG AGACGGACCG GAAAGATCTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCACTCC TGAGGAGCTC 
ATCCAGAAGC TGCAGTACAG ATATCCTTGC CGCCTCACCC TCCTTCACAG AGCCGTCTGC 
AGGGTGGGGT CAGCGATGAG GGGCAGTGCC CAGCCCAGCT CTGGTTCACT CAGGAAGGGA 
GGAGGAGGAG GAGGAGGGCG GCCTTCCCTT CACGTCCCCA CCCCTGCCCG CGAGGACGGC 
TGTTTCTTTA ACTCGGGCTC CACATACGAG AAGTTCTCAC CTTTCGCCGA CACATTCAAG 
AAGCGTGTGA GCAAGAACAC GTTCTTCGTG CTGGTGCGGG TGGTGGACGA GCTCTGCCTG 
GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTGGTGCGC 
AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC CGCAAGAACA TTCTGGACAA GGTCGACCAG 
AAGAAGCTGC TCCGGTGCGC CAACTCTGAC CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC 
AGGCCCGGGA CCTTGCACGA CTTCCACAGC CATGAAATAG CCGAGCAGCT GACTCTGCTA 
GATGCCGAGC TCTTTTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG 
AACGAGGAGA AGAGCCCGAA CCTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CGATAATCAT GTTGCAGGAA AAGGCCCAAG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTACGG AAGCTGAACA ACTTCAACTC CTACTTGGCC 
ATCCTCTCGG CGCTGGACTC GGCCCCCATT CGCAGGCTGG AGTGGCAGAA ACAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATC GACAGCTCGT CATCCTTCCG CGCCTACCGG 
GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC ATCCCATACC TGGGGCTCAT CCTGCAGGAC 
CTCACCTTCG TCCACCTGGG GAACCCAGAC TATATTGACG GGAAGGTGAA CTTCTCCAAA 
CGCTGGCAGC AGTTCAACAT CCTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTACGAC 
ATCCGGAGAA ATGACGACAT CATTAACTTC TTCAACGACT TCAGTGACCA CCTGGCCGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATC AAACCCAGGA ACATAACGAG GAGGAAGACA 
GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854442.1
CDS	344..4324
Translation	MGNAVEKQKPLKRSHLCPWKQDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998674.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998674.1

ORF Insert Sequence:

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCATC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCACGGCA GCTTACCTGT GCCCTCGTAC AAGTCCGTGT TTAGGTCCTA CTCGCAGGAT 
TTCGTGCCTC ACAGCCAAGC TTCCGTCCAG CCTTTCCTTC CGTCTACCTC CTCTTCCTCT 
CCACACTTCC CACCTGTCCA CCAGTATCAG AGCTCTGACT TAGCCGTGCC GCCCGTGGCC 
AGTCTGCCTC CCAGCACCCT GGACGGGCCT CTCTCATCTT CTCAGGAGAG CAGCTTTCAC 
GGGAACACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGCCCCA GACGCCTTCG 
AGTGAGCACG CCTGTGAGGG GGACTACGTC AGTCTGTATT GCTCTGGCCA GAGCAGCGAG 
GAGCTGGCCC GCCCTCGAGG AGAGTCACCC GCGGCGAAGG ATGGACACTC CAGAGACGCC 
ACATCGGCCA GCTGTGTGCC CGGGAAGGAG GGCAGGGACA GCGGGGACAG GGCCCGGAAG 
TCACCAGACG CTCCAGAGGC TCAGTCAGAG GAGGAGGTGG ACGAGCTCTC CCTCATCGAC 
CACAACGAGA TCATGGCGAG GCTGACCCTC AAGCAAGAGG GCGATGACGG GCCAGACGTC 
CGTGGAGGGT CGGGCGACAT CCTCCTCGTC CATGCCACGG AGACGGACCG GAAAGATCTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCACTCC TGAGGAGCTC 
ATCCAGAAGC TGCAGTACAG ATATCCTTGC CGCCTCACCC TCCTTCACAG AGCCGTCTGC 
AGGGTGGGGT CAGCGATGAG GGGCAGTGCC CAGCCCAGCT CTGGTTCACT CAGGAAGGGA 
GGAGGAGGAG GAGGAGGGCG GCCTTCCCTT CACGTCCCCA CCCCTGCCCG CGAGGACGGC 
TGTTTCTTTA ACTCGGGCTC CACATACGAG AAGTTCTCAC CTTTCGCCGA CACATTCAAG 
AAGCGTGTGA GCAAGAACAC GTTCTTCGTG CTGGTGCGGG TGGTGGACGA GCTCTGCCTG 
GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTGGTGCGC 
AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC CGCAAGAACA TTCTGGACAA GGTCGACCAG 
AAGAAGCTGC TCCGGTGCGC CAACTCTGAC CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC 
AGGCCCGGGA CCTTGCACGA CTTCCACAGC CATGAAATAG CCGAGCAGCT GACTCTGCTA 
GATGCCGAGC TCTTTTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG 
AACGAGGAGA AGAGCCCGAA CCTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CGATAATCAT GTTGCAGGAA AAGGCCCAAG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTACGG AAGCTGAACA ACTTCAACTC CTACTTGGCC 
ATCCTCTCGG CGCTGGACTC GGCCCCCATT CGCAGGCTGG AGTGGCAGAA ACAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATC GACAGCTCGT CATCCTTCCG CGCCTACCGG 
GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC ATCCCATACC TGGGGCTCAT CCTGCAGGAC 
CTCACCTTCG TCCACCTGGG GAACCCAGAC TATATTGACG GGAAGGTGAA CTTCTCCAAA 
CGCTGGCAGC AGTTCAACAT CCTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTACGAC 
ATCCGGAGAA ATGACGACAT CATTAACTTC TTCAACGACT TCAGTGACCA CCTGGCCGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATC AAACCCAGGA ACATAACGAG GAGGAAGACA 
GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998675.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854443.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220352
Clone ID Related Accession (Same CDS sequence)	XM_024998675.1
Accession Version	XM_024998675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCATCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TACCTGTGCC CTCGTACAAG TCCGTGTTTA GGTCCTACTC GCAGGATTTC 
GTGCCTCACA GCCAAGCTTC CGTCCAGCCT TTCCTTCCGT CTACCTCCTC TTCCTCTCCA 
CACTTCCCAC CTGTCCACCA GTATCAGAGC TCTGACTTAG CCGTGCCGCC CGTGGCCAGT 
CTGCCTCCCA GCACCCTGGA CGGGCCTCTC TCATCTTCTC AGGAGAGCAG CTTTCACGGG 
AACACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGCCCCAGAC GCCTTCGAGT 
GAGCACGCCT GTGAGGGGGA CTACGTCAGT CTGTATTGCT CTGGCCAGAG CAGCGAGGAG 
CTGGCCCGCC CTCGAGGAGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA TCCTTGCCGC CTCACCCTCC TTCACAGAGC CGTCTGCAGG 
GTGGGGTCAG CGATGAGGGG CAGTGCCCAG CCCAGCTCTG GTTCACTCAG GAAGGGAGGA 
GGAGGAGGAG GAGGGCGGCC TTCCCTTCAC GTCCCCACCC CTGCCCGCGA GGACGGCTGT 
TTCTTTAACT CGGGCTCCAC ATACGAGAAG TTCTCACCTT TCGCCGACAC ATTCAAGAAG 
CGTGTGAGCA AGAACACGTT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTA 
GAGCTGACAG AAGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT GGTGCGCAGC 
GGGGAGCTCA GCCTAGCACG CGTGCTCCGC AAGAACATTC TGGACAAGGT CGACCAGAAG 
AAGCTGCTCC GGTGCGCCAA CTCTGACCAG CCCCTGGCGG CCCGGGGAGT GGCAGCCAGG 
CCCGGGACCT TGCACGACTT CCACAGCCAT GAAATAGCCG AGCAGCTGAC TCTGCTAGAT 
GCCGAGCTCT TTTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCTAA AGAGCAGAAC 
GAGGAGAAGA GCCCGAACCT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCGA TAATCATGTT GCAGGAAAAG GCCCAAGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTACGGAAG CTGAACAACT TCAACTCCTA CTTGGCCATC 
CTCTCGGCGC TGGACTCGGC CCCCATTCGC AGGCTGGAGT GGCAGAAACA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATCGAC AGCTCGTCAT CCTTCCGCGC CTACCGGGCC 
GCCCTCTCGG AGGTAGAACC GCCCTGCATC CCATACCTGG GGCTCATCCT GCAGGACCTC 
ACCTTCGTCC ACCTGGGGAA CCCAGACTAT ATTGACGGGA AGGTGAACTT CTCCAAACGC 
TGGCAGCAGT TCAACATCCT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTACGACATC 
CGGAGAAATG ACGACATCAT TAACTTCTTC AACGACTTCA GTGACCACCT GGCCGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATCAAA CCCAGGAACA TAACGAGGAG GAAGACAGAT 
AGAGAAGAAA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854443.1
CDS	66..4043
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998675.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998675.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCATCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TACCTGTGCC CTCGTACAAG TCCGTGTTTA GGTCCTACTC GCAGGATTTC 
GTGCCTCACA GCCAAGCTTC CGTCCAGCCT TTCCTTCCGT CTACCTCCTC TTCCTCTCCA 
CACTTCCCAC CTGTCCACCA GTATCAGAGC TCTGACTTAG CCGTGCCGCC CGTGGCCAGT 
CTGCCTCCCA GCACCCTGGA CGGGCCTCTC TCATCTTCTC AGGAGAGCAG CTTTCACGGG 
AACACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGCCCCAGAC GCCTTCGAGT 
GAGCACGCCT GTGAGGGGGA CTACGTCAGT CTGTATTGCT CTGGCCAGAG CAGCGAGGAG 
CTGGCCCGCC CTCGAGGAGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA TCCTTGCCGC CTCACCCTCC TTCACAGAGC CGTCTGCAGG 
GTGGGGTCAG CGATGAGGGG CAGTGCCCAG CCCAGCTCTG GTTCACTCAG GAAGGGAGGA 
GGAGGAGGAG GAGGGCGGCC TTCCCTTCAC GTCCCCACCC CTGCCCGCGA GGACGGCTGT 
TTCTTTAACT CGGGCTCCAC ATACGAGAAG TTCTCACCTT TCGCCGACAC ATTCAAGAAG 
CGTGTGAGCA AGAACACGTT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTA 
GAGCTGACAG AAGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT GGTGCGCAGC 
GGGGAGCTCA GCCTAGCACG CGTGCTCCGC AAGAACATTC TGGACAAGGT CGACCAGAAG 
AAGCTGCTCC GGTGCGCCAA CTCTGACCAG CCCCTGGCGG CCCGGGGAGT GGCAGCCAGG 
CCCGGGACCT TGCACGACTT CCACAGCCAT GAAATAGCCG AGCAGCTGAC TCTGCTAGAT 
GCCGAGCTCT TTTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCTAA AGAGCAGAAC 
GAGGAGAAGA GCCCGAACCT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCGA TAATCATGTT GCAGGAAAAG GCCCAAGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTACGGAAG CTGAACAACT TCAACTCCTA CTTGGCCATC 
CTCTCGGCGC TGGACTCGGC CCCCATTCGC AGGCTGGAGT GGCAGAAACA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATCGAC AGCTCGTCAT CCTTCCGCGC CTACCGGGCC 
GCCCTCTCGG AGGTAGAACC GCCCTGCATC CCATACCTGG GGCTCATCCT GCAGGACCTC 
ACCTTCGTCC ACCTGGGGAA CCCAGACTAT ATTGACGGGA AGGTGAACTT CTCCAAACGC 
TGGCAGCAGT TCAACATCCT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTACGACATC 
CGGAGAAATG ACGACATCAT TAACTTCTTC AACGACTTCA GTGACCACCT GGCCGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATCAAA CCCAGGAACA TAACGAGGAG GAAGACAGAT 
AGAGAAGAAA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998676.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854444.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa220353
Clone ID Related Accession (Same CDS sequence)	XM_024998676.1
Accession Version	XM_024998676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATATC CTTGCCGCCT CACCCTCCTT 
CACAGAGCCG TCTGCAGGGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854444.1
CDS	128..4084
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998676.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998676.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATATC CTTGCCGCCT CACCCTCCTT 
CACAGAGCCG TCTGCAGGGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998677.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854445.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

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CloneID	OBa220354
Clone ID Related Accession (Same CDS sequence)	XM_024998677.1
Accession Version	XM_024998677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATATCC TTGCCGCCTC ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG 
GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC AGCTCTGGTT CACTCAGGAA GGGAGGAGGA 
GGAGGAGGAG GGCGGCCTTC CCTTCACGTC CCCACCCCTG CCCGCGAGGA CGGCTGTTTC 
TTTAACTCGG GCTCCACATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854445.1
CDS	127..4041
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998677.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998677.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATATCC TTGCCGCCTC ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG 
GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC AGCTCTGGTT CACTCAGGAA GGGAGGAGGA 
GGAGGAGGAG GGCGGCCTTC CCTTCACGTC CCCACCCCTG CCCGCGAGGA CGGCTGTTTC 
TTTAACTCGG GCTCCACATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998679.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854447.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854447.1
CDS	188..4072
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998679.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998679.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998680.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854448.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854448.1
CDS	226..4110
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998680.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998680.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998681.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854449.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

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CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854449.1
CDS	240..4124
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998681.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998681.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998678.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854446.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854446.1
CDS	354..4238
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998678.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998678.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998685.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854453.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854453.1
CDS	110..3994
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998685.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998685.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998686.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854454.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

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CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854454.1
CDS	239..4123
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998686.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998686.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998682.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854450.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa220355
Clone ID Related Accession (Same CDS sequence)	XM_024998679.1 , XM_024998680.1 , XM_024998681.1 , XM_024998678.1 , XM_024998685.1 , XM_024998686.1 , XM_024998682.1
Accession Version	XM_024998682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854450.1
CDS	58..3942
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998682.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998682.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATATCC TTGCCGCCTC 
ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC 
AGCTCTGGTT CACTCAGGAA GGGAGGAGGA GGAGGAGGAG GGCGGCCTTC CCTTCACGTC 
CCCACCCCTG CCCGCGAGGA CGGCTGTTTC TTTAACTCGG GCTCCACATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998687.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854455.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa220356
Clone ID Related Accession (Same CDS sequence)	XM_024998687.1
Accession Version	XM_024998687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATATCCTTG CCGCCTCACC 
CTCCTTCACA GAGCCGTCTG CAGGGTGGGG TCAGCGATGA GGGGCAGTGC CCAGCCCAGC 
TCTGGTTCAC TCAGGAAGGG AGGAGGAGGA GGAGGAGGGC GGCCTTCCCT TCACGTCCCC 
ACCCCTGCCC GCGAGGACGG CTGTTTCTTT AACTCGGGCT CCACATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854455.1
CDS	128..4009
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998687.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998687.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATATCCTTG CCGCCTCACC 
CTCCTTCACA GAGCCGTCTG CAGGGTGGGG TCAGCGATGA GGGGCAGTGC CCAGCCCAGC 
TCTGGTTCAC TCAGGAAGGG AGGAGGAGGA GGAGGAGGGC GGCCTTCCCT TCACGTCCCC 
ACCCCTGCCC GCGAGGACGG CTGTTTCTTT AACTCGGGCT CCACATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998688.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854456.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

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CloneID	OBa220357
Clone ID Related Accession (Same CDS sequence)	XM_024998688.1
Accession Version	XM_024998688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAT 
CCTTGCCGCC TCACCCTCCT TCACAGAGCC GTCTGCAGGG TGGGGTCAGC GATGAGGGGC 
AGTGCCCAGC CCAGCTCTGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854456.1
CDS	125..3961
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998688.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998688.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAT 
CCTTGCCGCC TCACCCTCCT TCACAGAGCC GTCTGCAGGG TGGGGTCAGC GATGAGGGGC 
AGTGCCCAGC CCAGCTCTGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998689.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854457.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa220358
Clone ID Related Accession (Same CDS sequence)	XM_024998689.1
Accession Version	XM_024998689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGCCTCCT GTGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC 
TCCTTTCTCA GCCGGCACGG CAGCTTACCT GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC 
TACTCGCAGG ATTTCGTGCC TCACAGCCAA GCTTCCGTCC AGCCTTTCCT TCCGTCTACC 
TCCTCTTCCT CTCCACACTT CCCACCTGTC CACCAGTATC AGAGCTCTGA CTTAGCCGTG 
CCGCCCGTGG CCAGTCTGCC TCCCAGCACC CTGGACGGGC CTCTCTCATC TTCTCAGGAG 
AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC 
CAGACGCCTT CGGAGTCACC CGCGGCGAAG GATGGACACT CCAGAGACGC CACATCGGCC 
AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC AGCGGGGACA GGGCCCGGAA GTCACCAGAC 
GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG GACGAGCTCT CCCTCATCGA CCACAACGAG 
ATCATGGCGA GGCTGACCCT CAAGCAAGAG GGCGATGACG GGCCAGACGT CCGTGGAGGG 
TCGGGCGACA TCCTCCTCGT CCATGCCACG GAGACGGACC GGAAAGATCT GGTGTTGTAC 
TGCGAGGCCT TCCTGACCAC CTACAGGACC TTCATCACTC CTGAGGAGCT CATCCAGAAG 
CTGCAGTACA GATACGAGAA GTTCTCACCT TTCGCCGACA CATTCAAGAA GCGTGTGAGC 
AAGAACACGT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT AGAGCTGACA 
GAAGAGATCC TGAAGCTGCT GATGGAGCTG GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC 
AGCCTAGCAC GCGTGCTCCG CAAGAACATT CTGGACAAGG TCGACCAGAA GAAGCTGCTC 
CGGTGCGCCA ACTCTGACCA GCCCCTGGCG GCCCGGGGAG TGGCAGCCAG GCCCGGGACC 
TTGCACGACT TCCACAGCCA TGAAATAGCC GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC 
TTTTATAAAA TAGAGATTCC TGAGGTTTTG CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG 
AGCCCGAACC TGACCCAGTT CACGGAGCAC TTCAACAACA TGTCCTACTG GGTCCGGTCG 
ATAATCATGT TGCAGGAAAA GGCCCAAGAC AGGGAACGGC TGCTCTTGAA GTTCATCAAG 
ATCATGAAGC ACTTACGGAA GCTGAACAAC TTCAACTCCT ACTTGGCCAT CCTCTCGGCG 
CTGGACTCGG CCCCCATTCG CAGGCTGGAG TGGCAGAAAC AGACCTCAGA GGGCCTGGCC 
GAGTACTGCA CGCTGATCGA CAGCTCGTCA TCCTTCCGCG CCTACCGGGC CGCCCTCTCG 
GAGGTAGAAC CGCCCTGCAT CCCATACCTG GGGCTCATCC TGCAGGACCT CACCTTCGTC 
CACCTGGGGA ACCCAGACTA TATTGACGGG AAGGTGAACT TCTCCAAACG CTGGCAGCAG 
TTCAACATCC TGGACAGCAT GCGGTGCTTC CAGCAGGCGC ACTACGACAT CCGGAGAAAT 
GACGACATCA TTAACTTCTT CAACGACTTC AGTGACCACC TGGCCGAGGA GGCCCTATGG 
GAACTCTCTC TGAAAATCAA ACCCAGGAAC ATAACGAGGA GGAAGACAGA TAGAGAAGAA 
AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854457.1
CDS	121..3849
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998689.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998689.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGCCTCCT GTGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC 
TCCTTTCTCA GCCGGCACGG CAGCTTACCT GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC 
TACTCGCAGG ATTTCGTGCC TCACAGCCAA GCTTCCGTCC AGCCTTTCCT TCCGTCTACC 
TCCTCTTCCT CTCCACACTT CCCACCTGTC CACCAGTATC AGAGCTCTGA CTTAGCCGTG 
CCGCCCGTGG CCAGTCTGCC TCCCAGCACC CTGGACGGGC CTCTCTCATC TTCTCAGGAG 
AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC 
CAGACGCCTT CGGAGTCACC CGCGGCGAAG GATGGACACT CCAGAGACGC CACATCGGCC 
AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC AGCGGGGACA GGGCCCGGAA GTCACCAGAC 
GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG GACGAGCTCT CCCTCATCGA CCACAACGAG 
ATCATGGCGA GGCTGACCCT CAAGCAAGAG GGCGATGACG GGCCAGACGT CCGTGGAGGG 
TCGGGCGACA TCCTCCTCGT CCATGCCACG GAGACGGACC GGAAAGATCT GGTGTTGTAC 
TGCGAGGCCT TCCTGACCAC CTACAGGACC TTCATCACTC CTGAGGAGCT CATCCAGAAG 
CTGCAGTACA GATACGAGAA GTTCTCACCT TTCGCCGACA CATTCAAGAA GCGTGTGAGC 
AAGAACACGT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT AGAGCTGACA 
GAAGAGATCC TGAAGCTGCT GATGGAGCTG GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC 
AGCCTAGCAC GCGTGCTCCG CAAGAACATT CTGGACAAGG TCGACCAGAA GAAGCTGCTC 
CGGTGCGCCA ACTCTGACCA GCCCCTGGCG GCCCGGGGAG TGGCAGCCAG GCCCGGGACC 
TTGCACGACT TCCACAGCCA TGAAATAGCC GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC 
TTTTATAAAA TAGAGATTCC TGAGGTTTTG CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG 
AGCCCGAACC TGACCCAGTT CACGGAGCAC TTCAACAACA TGTCCTACTG GGTCCGGTCG 
ATAATCATGT TGCAGGAAAA GGCCCAAGAC AGGGAACGGC TGCTCTTGAA GTTCATCAAG 
ATCATGAAGC ACTTACGGAA GCTGAACAAC TTCAACTCCT ACTTGGCCAT CCTCTCGGCG 
CTGGACTCGG CCCCCATTCG CAGGCTGGAG TGGCAGAAAC AGACCTCAGA GGGCCTGGCC 
GAGTACTGCA CGCTGATCGA CAGCTCGTCA TCCTTCCGCG CCTACCGGGC CGCCCTCTCG 
GAGGTAGAAC CGCCCTGCAT CCCATACCTG GGGCTCATCC TGCAGGACCT CACCTTCGTC 
CACCTGGGGA ACCCAGACTA TATTGACGGG AAGGTGAACT TCTCCAAACG CTGGCAGCAG 
TTCAACATCC TGGACAGCAT GCGGTGCTTC CAGCAGGCGC ACTACGACAT CCGGAGAAAT 
GACGACATCA TTAACTTCTT CAACGACTTC AGTGACCACC TGGCCGAGGA GGCCCTATGG 
GAACTCTCTC TGAAAATCAA ACCCAGGAAC ATAACGAGGA GGAAGACAGA TAGAGAAGAA 
AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998690.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854458.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa220359
Clone ID Related Accession (Same CDS sequence)	XM_024998690.1
Accession Version	XM_024998690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATATCCTTG CCGCCTCACC 
CTCCTTCACA GAGCCGTCTG CAGGGTGGGG TCAGCGATGA GGGGCAGTGC CCAGCCCAGC 
TCTGGTTCAC TCAGGAAGGG AGGAGGAGGA GGAGGAGGGC GGCCTTCCCT TCACGTCCCC 
ACCCCTGCCC GCGAGGACGG CTGTTTCTTT AACTCGGGCT CCACATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854458.1
CDS	121..3762
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998690.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998690.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATATCCTTG CCGCCTCACC 
CTCCTTCACA GAGCCGTCTG CAGGGTGGGG TCAGCGATGA GGGGCAGTGC CCAGCCCAGC 
TCTGGTTCAC TCAGGAAGGG AGGAGGAGGA GGAGGAGGGC GGCCTTCCCT TCACGTCCCC 
ACCCCTGCCC GCGAGGACGG CTGTTTCTTT AACTCGGGCT CCACATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998692.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854460.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

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CloneID	OBa220360
Clone ID Related Accession (Same CDS sequence)	XM_024998692.1
Accession Version	XM_024998692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGAGTGAGC ACGCCTGTGA GGGGGACTAC GTCAGTCTGT ATTGCTCTGG CCAGAGCAGC 
GAGGAGCTGG CCCGCCCTCG AGGAGAGTCA CCCGCGGCGA AGGATGGACA CTCCAGAGAC 
GCCACATCGG CCAGCTGTGT GCCCGGGAAG GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG 
AAGTCACCAG ACGCTCCAGA GGCTCAGTCA GAGGAGGAGG TGGACGAGCT CTCCCTCATC 
GACCACAACG AGATCATGGC GAGGCTGACC CTCAAGCAAG AGGGCGATGA CGGGCCAGAC 
GTCCGTGGAG GGTCGGGCGA CATCCTCCTC GTCCATGCCA CGGAGACGGA CCGGAAAGAT 
CTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCAC TCCTGAGGAG 
CTCATCCAGA AGCTGCAGTA CAGATATCCT TGCCGCCTCA CCCTCCTTCA CAGAGCCGTC 
TGCAGGGTGG GGTCAGCGAT GAGGGGCAGT GCCCAGCCCA GCTCTGGTTC ACTCAGGAAG 
GGAGGAGGAG GAGGAGGAGG GCGGCCTTCC CTTCACGTCC CCACCCCTGC CCGCGAGGAC 
GGCTGTTTCT TTAACTCGGG CTCCACATAC GAGAAGTTCT CACCTTTCGC CGACACATTC 
AAGAAGCGTG TGAGCAAGAA CACGTTCTTC GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC 
CTGGTAGAGC TGACAGAAGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTGGTG 
CGCAGCGGGG AGCTCAGCCT AGCACGCGTG CTCCGCAAGA ACATTCTGGA CAAGGTCGAC 
CAGAAGAAGC TGCTCCGGTG CGCCAACTCT GACCAGCCCC TGGCGGCCCG GGGAGTGGCA 
GCCAGGCCCG GGACCTTGCA CGACTTCCAC AGCCATGAAA TAGCCGAGCA GCTGACTCTG 
CTAGATGCCG AGCTCTTTTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG 
CAGAACGAGG AGAAGAGCCC GAACCTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCGATAAT CATGTTGCAG GAAAAGGCCC AAGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTA CGGAAGCTGA ACAACTTCAA CTCCTACTTG 
GCCATCCTCT CGGCGCTGGA CTCGGCCCCC ATTCGCAGGC TGGAGTGGCA GAAACAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATCGACAGCT CGTCATCCTT CCGCGCCTAC 
CGGGCCGCCC TCTCGGAGGT AGAACCGCCC TGCATCCCAT ACCTGGGGCT CATCCTGCAG 
GACCTCACCT TCGTCCACCT GGGGAACCCA GACTATATTG ACGGGAAGGT GAACTTCTCC 
AAACGCTGGC AGCAGTTCAA CATCCTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAC 
GACATCCGGA GAAATGACGA CATCATTAAC TTCTTCAACG ACTTCAGTGA CCACCTGGCC 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATCAAACCCA GGAACATAAC GAGGAGGAAG 
ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854460.1
CDS	119..3682
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998692.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998692.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGAGTGAGC ACGCCTGTGA GGGGGACTAC GTCAGTCTGT ATTGCTCTGG CCAGAGCAGC 
GAGGAGCTGG CCCGCCCTCG AGGAGAGTCA CCCGCGGCGA AGGATGGACA CTCCAGAGAC 
GCCACATCGG CCAGCTGTGT GCCCGGGAAG GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG 
AAGTCACCAG ACGCTCCAGA GGCTCAGTCA GAGGAGGAGG TGGACGAGCT CTCCCTCATC 
GACCACAACG AGATCATGGC GAGGCTGACC CTCAAGCAAG AGGGCGATGA CGGGCCAGAC 
GTCCGTGGAG GGTCGGGCGA CATCCTCCTC GTCCATGCCA CGGAGACGGA CCGGAAAGAT 
CTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCAC TCCTGAGGAG 
CTCATCCAGA AGCTGCAGTA CAGATATCCT TGCCGCCTCA CCCTCCTTCA CAGAGCCGTC 
TGCAGGGTGG GGTCAGCGAT GAGGGGCAGT GCCCAGCCCA GCTCTGGTTC ACTCAGGAAG 
GGAGGAGGAG GAGGAGGAGG GCGGCCTTCC CTTCACGTCC CCACCCCTGC CCGCGAGGAC 
GGCTGTTTCT TTAACTCGGG CTCCACATAC GAGAAGTTCT CACCTTTCGC CGACACATTC 
AAGAAGCGTG TGAGCAAGAA CACGTTCTTC GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC 
CTGGTAGAGC TGACAGAAGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTGGTG 
CGCAGCGGGG AGCTCAGCCT AGCACGCGTG CTCCGCAAGA ACATTCTGGA CAAGGTCGAC 
CAGAAGAAGC TGCTCCGGTG CGCCAACTCT GACCAGCCCC TGGCGGCCCG GGGAGTGGCA 
GCCAGGCCCG GGACCTTGCA CGACTTCCAC AGCCATGAAA TAGCCGAGCA GCTGACTCTG 
CTAGATGCCG AGCTCTTTTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG 
CAGAACGAGG AGAAGAGCCC GAACCTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCGATAAT CATGTTGCAG GAAAAGGCCC AAGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTA CGGAAGCTGA ACAACTTCAA CTCCTACTTG 
GCCATCCTCT CGGCGCTGGA CTCGGCCCCC ATTCGCAGGC TGGAGTGGCA GAAACAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATCGACAGCT CGTCATCCTT CCGCGCCTAC 
CGGGCCGCCC TCTCGGAGGT AGAACCGCCC TGCATCCCAT ACCTGGGGCT CATCCTGCAG 
GACCTCACCT TCGTCCACCT GGGGAACCCA GACTATATTG ACGGGAAGGT GAACTTCTCC 
AAACGCTGGC AGCAGTTCAA CATCCTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAC 
GACATCCGGA GAAATGACGA CATCATTAAC TTCTTCAACG ACTTCAGTGA CCACCTGGCC 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATCAAACCCA GGAACATAAC GAGGAGGAAG 
ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998693.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854461.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa220361
Clone ID Related Accession (Same CDS sequence)	XM_024998693.1
Accession Version	XM_024998693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTAG TCATTCTGAG ACAGTGGGCT GGTCATTTCC CCCTTCCAAG AGGAAGTGTC 
TTTATGCATG CGAAACTTCT CTGCGCGCTG TGGAGGTGTT TTGGGCGGGT AGGACATGTT 
TTGGGAAGTC ATGAAGATTA CCTGAGTCAA ACAATACTCG CCCATAGGGA GCTGGTCAGA 
CTCACCATCG AGAAGCAGGG GCATCCATCT CCAACAAGCC CTGTGAAACC CAGCTCCCCA 
GCCTGCAAAC CTGACAGCCA GTCTGAGCTC CCCCTGACGG ATCGAGAGAT GGAGATACTA 
AACAAGACGA CCAGCCTGTC ACAGTCAACC GAGGGGCTCC AAGACTCTAT GGACGAAGAG 
GTTGCACCCC CCAAGCCCCC CTTACCTGGC ATTAGGGTGG TTGATAACAG TCCTCCACCA 
GCACTGCCCC CGAAGAAGAG ACAGTCGGCT CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC 
CCCATGAGTC GAGCCACCAG TGGCTCCAGT TTGCCTGTTG GAATTAATAG GCAGGACTTT 
GACGTGGACT GTTACGCACA GAGACGGCTG TCGGGAGGCA GCCACTCGTA CAGCGGTGAG 
TCTCCCCGCC TGTCGCCCTG CAGCAGCATC GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG 
TCCTCTCTGG ACAGGGACAG CGGGCAGTGC TCCCGGAACA CAAGCTGTGA AACACTAGAT 
CATTACGATC CTGACTACGA GTTCCTTCAG CAAGACCTCT CCAACGCAGA CCAGATACCT 
CAGCAAGTGG CCTGTAACCT CAGCCCGCTG CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC 
TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA 
GCCCCGGGGC AGCAGACGGA CGTGCCGCCA GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA 
GCCTCCCAGA CCTTGGACAG CTCTGGCTGC CGGGCGTCCT ACGAGCGACA CCCCTCACAG 
TACGACAACA TCTCCGAGGA TGACCTGCAG AACCCAGCCT CCGTCCAGTC CGTCCCCTTC 
ACGCCCTTTG CTGCTATTCT TCCCTTTCAG CAAGGAGGTT CCTCAGCCTC TGTCGACTTC 
ATGGGTGATT TCACTGTTTC TGAATCAACG GGTGACCCAG AAAAACCGCC ACCTCTCCCC 
GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAGCTGC TTGAGGACTA CTCGGAGCCA 
CAGCCTTCCA TGTTCTACCA GACGCCGCAG AAGGAACACG TGTACCAGCA GAAGAACAAG 
CTTCTCATGG AGGTGTATGG CTTCAACGAC TCCTTCAGCA CCGGCGACGC CGCACACGAT 
CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT 
TCTTCCTTCT CCTCTGTCTC CTACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG 
GACGGCAGTG CCGCTCAGGG CATCAGTGTG TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC 
GGCAGCTTAC CTGTGCCCTC GTACAAGTCC GTGTTTAGGT CCTACTCGCA GGATTTCGTG 
CCTCACAGCC AAGCTTCCGT CCAGCCTTTC CTTCCGTCTA CCTCCTCTTC CTCTCCACAC 
TTCCCACCTG TCCACCAGTA TCAGAGCTCT GACTTAGCCG TGCCGCCCGT GGCCAGTCTG 
CCTCCCAGCA CCCTGGACGG GCCTCTCTCA TCTTCTCAGG AGAGCAGCTT TCACGGGAAC 
ACTGTCTGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGC CCCAGACGCC TTCGAGTGAG 
CACGCCTGTG AGGGGGACTA CGTCAGTCTG TATTGCTCTG GCCAGAGCAG CGAGGAGCTG 
GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATATCC TTGCCGCCTC ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG 
GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC AGCTCTGGTT CACTCAGGAA GGGAGGAGGA 
GGAGGAGGAG GGCGGCCTTC CCTTCACGTC CCCACCCCTG CCCGCGAGGA CGGCTGTTTC 
TTTAACTCGG GCTCCACATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854461.1
CDS	419..3913
Translation	MNLVILRQWAGHFPLPRGSVFMHAKLLCALWRCFGRVGHVLGSHEDYLSQTILAHRELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998693.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998693.1

ORF Insert Sequence:

ATGAATTTAG TCATTCTGAG ACAGTGGGCT GGTCATTTCC CCCTTCCAAG AGGAAGTGTC 
TTTATGCATG CGAAACTTCT CTGCGCGCTG TGGAGGTGTT TTGGGCGGGT AGGACATGTT 
TTGGGAAGTC ATGAAGATTA CCTGAGTCAA ACAATACTCG CCCATAGGGA GCTGGTCAGA 
CTCACCATCG AGAAGCAGGG GCATCCATCT CCAACAAGCC CTGTGAAACC CAGCTCCCCA 
GCCTGCAAAC CTGACAGCCA GTCTGAGCTC CCCCTGACGG ATCGAGAGAT GGAGATACTA 
AACAAGACGA CCAGCCTGTC ACAGTCAACC GAGGGGCTCC AAGACTCTAT GGACGAAGAG 
GTTGCACCCC CCAAGCCCCC CTTACCTGGC ATTAGGGTGG TTGATAACAG TCCTCCACCA 
GCACTGCCCC CGAAGAAGAG ACAGTCGGCT CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC 
CCCATGAGTC GAGCCACCAG TGGCTCCAGT TTGCCTGTTG GAATTAATAG GCAGGACTTT 
GACGTGGACT GTTACGCACA GAGACGGCTG TCGGGAGGCA GCCACTCGTA CAGCGGTGAG 
TCTCCCCGCC TGTCGCCCTG CAGCAGCATC GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG 
TCCTCTCTGG ACAGGGACAG CGGGCAGTGC TCCCGGAACA CAAGCTGTGA AACACTAGAT 
CATTACGATC CTGACTACGA GTTCCTTCAG CAAGACCTCT CCAACGCAGA CCAGATACCT 
CAGCAAGTGG CCTGTAACCT CAGCCCGCTG CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC 
TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA 
GCCCCGGGGC AGCAGACGGA CGTGCCGCCA GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA 
GCCTCCCAGA CCTTGGACAG CTCTGGCTGC CGGGCGTCCT ACGAGCGACA CCCCTCACAG 
TACGACAACA TCTCCGAGGA TGACCTGCAG AACCCAGCCT CCGTCCAGTC CGTCCCCTTC 
ACGCCCTTTG CTGCTATTCT TCCCTTTCAG CAAGGAGGTT CCTCAGCCTC TGTCGACTTC 
ATGGGTGATT TCACTGTTTC TGAATCAACG GGTGACCCAG AAAAACCGCC ACCTCTCCCC 
GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAGCTGC TTGAGGACTA CTCGGAGCCA 
CAGCCTTCCA TGTTCTACCA GACGCCGCAG AAGGAACACG TGTACCAGCA GAAGAACAAG 
CTTCTCATGG AGGTGTATGG CTTCAACGAC TCCTTCAGCA CCGGCGACGC CGCACACGAT 
CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT 
TCTTCCTTCT CCTCTGTCTC CTACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG 
GACGGCAGTG CCGCTCAGGG CATCAGTGTG TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC 
GGCAGCTTAC CTGTGCCCTC GTACAAGTCC GTGTTTAGGT CCTACTCGCA GGATTTCGTG 
CCTCACAGCC AAGCTTCCGT CCAGCCTTTC CTTCCGTCTA CCTCCTCTTC CTCTCCACAC 
TTCCCACCTG TCCACCAGTA TCAGAGCTCT GACTTAGCCG TGCCGCCCGT GGCCAGTCTG 
CCTCCCAGCA CCCTGGACGG GCCTCTCTCA TCTTCTCAGG AGAGCAGCTT TCACGGGAAC 
ACTGTCTGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGC CCCAGACGCC TTCGAGTGAG 
CACGCCTGTG AGGGGGACTA CGTCAGTCTG TATTGCTCTG GCCAGAGCAG CGAGGAGCTG 
GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATATCC TTGCCGCCTC ACCCTCCTTC ACAGAGCCGT CTGCAGGGTG 
GGGTCAGCGA TGAGGGGCAG TGCCCAGCCC AGCTCTGGTT CACTCAGGAA GGGAGGAGGA 
GGAGGAGGAG GGCGGCCTTC CCTTCACGTC CCCACCCCTG CCCGCGAGGA CGGCTGTTTC 
TTTAACTCGG GCTCCACATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998694.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854462.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OBa220362
Clone ID Related Accession (Same CDS sequence)	XM_024998694.1
Accession Version	XM_024998694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATATCCTT GCCGCCTCAC CCTCCTTCAC AGAGCCGTCT GCAGGGTGGG GTCAGCGATG 
AGGGGCAGTG CCCAGCCCAG CTCTGGTTCA CTCAGGAAGG GAGGAGGAGG AGGAGGAGGG 
CGGCCTTCCC TTCACGTCCC CACCCCTGCC CGCGAGGACG GCTGTTTCTT TAACTCGGGC 
TCCACATACG AGAAGTTCTC ACCTTTCGCC GACACATTCA AGAAGCGTGT GAGCAAGAAC 
ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC GAGCTCTGCC TGGTAGAGCT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA GCTGGTCTTC CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA 
GCACGCGTGC TCCGCAAGAA CATTCTGGAC AAGGTCGACC AGAAGAAGCT GCTCCGGTGC 
GCCAACTCTG ACCAGCCCCT GGCGGCCCGG GGAGTGGCAG CCAGGCCCGG GACCTTGCAC 
GACTTCCACA GCCATGAAAT AGCCGAGCAG CTGACTCTGC TAGATGCCGA GCTCTTTTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCTAAAGAGC AGAACGAGGA GAAGAGCCCG 
AACCTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCGATAATC 
ATGTTGCAGG AAAAGGCCCA AGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTAC GGAAGCTGAA CAACTTCAAC TCCTACTTGG CCATCCTCTC GGCGCTGGAC 
TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG AAACAGACCT CAGAGGGCCT GGCCGAGTAC 
TGCACGCTGA TCGACAGCTC GTCATCCTTC CGCGCCTACC GGGCCGCCCT CTCGGAGGTA 
GAACCGCCCT GCATCCCATA CCTGGGGCTC ATCCTGCAGG ACCTCACCTT CGTCCACCTG 
GGGAACCCAG ACTATATTGA CGGGAAGGTG AACTTCTCCA AACGCTGGCA GCAGTTCAAC 
ATCCTGGACA GCATGCGGTG CTTCCAGCAG GCGCACTACG ACATCCGGAG AAATGACGAC 
ATCATTAACT TCTTCAACGA CTTCAGTGAC CACCTGGCCG AGGAGGCCCT ATGGGAACTC 
TCTCTGAAAA TCAAACCCAG GAACATAACG AGGAGGAAGA CAGATAGAGA AGAAAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854462.1
CDS	115..3597
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998694.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998694.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATATCCTT GCCGCCTCAC CCTCCTTCAC AGAGCCGTCT GCAGGGTGGG GTCAGCGATG 
AGGGGCAGTG CCCAGCCCAG CTCTGGTTCA CTCAGGAAGG GAGGAGGAGG AGGAGGAGGG 
CGGCCTTCCC TTCACGTCCC CACCCCTGCC CGCGAGGACG GCTGTTTCTT TAACTCGGGC 
TCCACATACG AGAAGTTCTC ACCTTTCGCC GACACATTCA AGAAGCGTGT GAGCAAGAAC 
ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC GAGCTCTGCC TGGTAGAGCT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA GCTGGTCTTC CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA 
GCACGCGTGC TCCGCAAGAA CATTCTGGAC AAGGTCGACC AGAAGAAGCT GCTCCGGTGC 
GCCAACTCTG ACCAGCCCCT GGCGGCCCGG GGAGTGGCAG CCAGGCCCGG GACCTTGCAC 
GACTTCCACA GCCATGAAAT AGCCGAGCAG CTGACTCTGC TAGATGCCGA GCTCTTTTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCTAAAGAGC AGAACGAGGA GAAGAGCCCG 
AACCTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCGATAATC 
ATGTTGCAGG AAAAGGCCCA AGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTAC GGAAGCTGAA CAACTTCAAC TCCTACTTGG CCATCCTCTC GGCGCTGGAC 
TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG AAACAGACCT CAGAGGGCCT GGCCGAGTAC 
TGCACGCTGA TCGACAGCTC GTCATCCTTC CGCGCCTACC GGGCCGCCCT CTCGGAGGTA 
GAACCGCCCT GCATCCCATA CCTGGGGCTC ATCCTGCAGG ACCTCACCTT CGTCCACCTG 
GGGAACCCAG ACTATATTGA CGGGAAGGTG AACTTCTCCA AACGCTGGCA GCAGTTCAAC 
ATCCTGGACA GCATGCGGTG CTTCCAGCAG GCGCACTACG ACATCCGGAG AAATGACGAC 
ATCATTAACT TCTTCAACGA CTTCAGTGAC CACCTGGCCG AGGAGGCCCT ATGGGAACTC 
TCTCTGAAAA TCAAACCCAG GAACATAACG AGGAGGAAGA CAGATAGAGA AGAAAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998695.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854463.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa220363
Clone ID Related Accession (Same CDS sequence)	XM_024998695.1
Accession Version	XM_024998695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCA ACACTGTGGA CAGACTAAAA CCCGCTGGAT TGCACGCTTT AAATGGCTGG 
ATTTTATGGC GTAAAGCTGT TCAGAAAAAG GAGCTGGTCA GACTCACCAT CGAGAAGCAG 
GGGCATCCAT CTCCAACAAG CCCTGTGAAA CCCAGCTCCC CAGCCTGCAA ACCTGACAGC 
CAGTCTGAGC TCCCCCTGAC GGATCGAGAG ATGGAGATAC TAAACAAGAC GACCAGCCTG 
TCACAGTCAA CCGAGGGGCT CCAAGACTCT ATGGACGAAG AGGTTGCACC CCCCAAGCCC 
CCCTTACCTG GCATTAGGGT GGTTGATAAC AGTCCTCCAC CAGCACTGCC CCCGAAGAAG 
AGACAGTCGG CTCCGTCCCC CACGCGTGTG GCCGTTGTGG CCCCCATGAG TCGAGCCACC 
AGTGGCTCCA GTTTGCCTGT TGGAATTAAT AGGCAGGACT TTGACGTGGA CTGTTACGCA 
CAGAGACGGC TGTCGGGAGG CAGCCACTCG TACAGCGGTG AGTCTCCCCG CCTGTCGCCC 
TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG GACGAGCAGC TGTCCTCTCT GGACAGGGAC 
AGCGGGCAGT GCTCCCGGAA CACAAGCTGT GAAACACTAG ATCATTACGA TCCTGACTAC 
GAGTTCCTTC AGCAAGACCT CTCCAACGCA GACCAGATAC CTCAGCAAGT GGCCTGTAAC 
CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG TCTGGGTCTC CCTTTCTTGG CCATCCTCTC 
CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA GATGGGTCCT CAGCCCCGGG GCAGCAGACG 
GACGTGCCGC CAGCCCTGCC CGAGAAGAAG CGCAGGAGTG CAGCCTCCCA GACCTTGGAC 
AGCTCTGGCT GCCGGGCGTC CTACGAGCGA CACCCCTCAC AGTACGACAA CATCTCCGAG 
GATGACCTGC AGAACCCAGC CTCCGTCCAG TCCGTCCCCT TCACGCCCTT TGCTGCTATT 
CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC TCTGTCGACT TCATGGGTGA TTTCACTGTT 
TCTGAATCAA CGGGTGACCC AGAAAAACCG CCACCTCTCC CCGAGAAGAA AAACAAACAC 
ATGCTGGCCT ACATGCAGCT GCTTGAGGAC TACTCGGAGC CACAGCCTTC CATGTTCTAC 
CAGACGCCGC AGAAGGAACA CGTGTACCAG CAGAAGAACA AGCTTCTCAT GGAGGTGTAT 
GGCTTCAACG ACTCCTTCAG CACCGGCGAC GCCGCACACG ATCTCGCCCC GCCCCCCGCC 
CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCATCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC 
TCGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAT 
CCTTGCCGCC TCACCCTCCT TCACAGAGCC GTCTGCAGGG TGGGGTCAGC GATGAGGGGC 
AGTGCCCAGC CCAGCTCTGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854463.1
CDS	500..3916
Translation	MVPNTVDRLKPAGLHALNGWILWRKAVQKKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998695.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998695.1

ORF Insert Sequence:

ATGGTGCCCA ACACTGTGGA CAGACTAAAA CCCGCTGGAT TGCACGCTTT AAATGGCTGG 
ATTTTATGGC GTAAAGCTGT TCAGAAAAAG GAGCTGGTCA GACTCACCAT CGAGAAGCAG 
GGGCATCCAT CTCCAACAAG CCCTGTGAAA CCCAGCTCCC CAGCCTGCAA ACCTGACAGC 
CAGTCTGAGC TCCCCCTGAC GGATCGAGAG ATGGAGATAC TAAACAAGAC GACCAGCCTG 
TCACAGTCAA CCGAGGGGCT CCAAGACTCT ATGGACGAAG AGGTTGCACC CCCCAAGCCC 
CCCTTACCTG GCATTAGGGT GGTTGATAAC AGTCCTCCAC CAGCACTGCC CCCGAAGAAG 
AGACAGTCGG CTCCGTCCCC CACGCGTGTG GCCGTTGTGG CCCCCATGAG TCGAGCCACC 
AGTGGCTCCA GTTTGCCTGT TGGAATTAAT AGGCAGGACT TTGACGTGGA CTGTTACGCA 
CAGAGACGGC TGTCGGGAGG CAGCCACTCG TACAGCGGTG AGTCTCCCCG CCTGTCGCCC 
TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG GACGAGCAGC TGTCCTCTCT GGACAGGGAC 
AGCGGGCAGT GCTCCCGGAA CACAAGCTGT GAAACACTAG ATCATTACGA TCCTGACTAC 
GAGTTCCTTC AGCAAGACCT CTCCAACGCA GACCAGATAC CTCAGCAAGT GGCCTGTAAC 
CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG TCTGGGTCTC CCTTTCTTGG CCATCCTCTC 
CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA GATGGGTCCT CAGCCCCGGG GCAGCAGACG 
GACGTGCCGC CAGCCCTGCC CGAGAAGAAG CGCAGGAGTG CAGCCTCCCA GACCTTGGAC 
AGCTCTGGCT GCCGGGCGTC CTACGAGCGA CACCCCTCAC AGTACGACAA CATCTCCGAG 
GATGACCTGC AGAACCCAGC CTCCGTCCAG TCCGTCCCCT TCACGCCCTT TGCTGCTATT 
CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC TCTGTCGACT TCATGGGTGA TTTCACTGTT 
TCTGAATCAA CGGGTGACCC AGAAAAACCG CCACCTCTCC CCGAGAAGAA AAACAAACAC 
ATGCTGGCCT ACATGCAGCT GCTTGAGGAC TACTCGGAGC CACAGCCTTC CATGTTCTAC 
CAGACGCCGC AGAAGGAACA CGTGTACCAG CAGAAGAACA AGCTTCTCAT GGAGGTGTAT 
GGCTTCAACG ACTCCTTCAG CACCGGCGAC GCCGCACACG ATCTCGCCCC GCCCCCCGCC 
CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCATCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC 
TCGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAT 
CCTTGCCGCC TCACCCTCCT TCACAGAGCC GTCTGCAGGG TGGGGTCAGC GATGAGGGGC 
AGTGCCCAGC CCAGCTCTGG TTCACTCAGG AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT 
TCCCTTCACG TCCCCACCCC TGCCCGCGAG GACGGCTGTT TCTTTAACTC GGGCTCCACA 
TACGAGAAGT TCTCACCTTT CGCCGACACA TTCAAGAAGC GTGTGAGCAA GAACACGTTC 
TTCGTGCTGG TGCGGGTGGT GGACGAGCTC TGCCTGGTAG AGCTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG GTGCGCAGCG GGGAGCTCAG CCTAGCACGC 
GTGCTCCGCA AGAACATTCT GGACAAGGTC GACCAGAAGA AGCTGCTCCG GTGCGCCAAC 
TCTGACCAGC CCCTGGCGGC CCGGGGAGTG GCAGCCAGGC CCGGGACCTT GCACGACTTC 
CACAGCCATG AAATAGCCGA GCAGCTGACT CTGCTAGATG CCGAGCTCTT TTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCTAAA GAGCAGAACG AGGAGAAGAG CCCGAACCTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCGAT AATCATGTTG 
CAGGAAAAGG CCCAAGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTACGGAAGC TGAACAACTT CAACTCCTAC TTGGCCATCC TCTCGGCGCT GGACTCGGCC 
CCCATTCGCA GGCTGGAGTG GCAGAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACG 
CTGATCGACA GCTCGTCATC CTTCCGCGCC TACCGGGCCG CCCTCTCGGA GGTAGAACCG 
CCCTGCATCC CATACCTGGG GCTCATCCTG CAGGACCTCA CCTTCGTCCA CCTGGGGAAC 
CCAGACTATA TTGACGGGAA GGTGAACTTC TCCAAACGCT GGCAGCAGTT CAACATCCTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TACGACATCC GGAGAAATGA CGACATCATT 
AACTTCTTCA ACGACTTCAG TGACCACCTG GCCGAGGAGG CCCTATGGGA ACTCTCTCTG 
AAAATCAAAC CCAGGAACAT AACGAGGAGG AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998696.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854464.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa220364
Clone ID Related Accession (Same CDS sequence)	XM_024998696.1
Accession Version	XM_024998696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC TCGTACAAGT CCGTGTTTAG GTCCTACTCG 
CAGGATTTCG TGCCTCACAG CCAAGCTTCC GTCCAGCCTT TCCTTCCGTC TACCTCCTCT 
TCCTCTCCAC ACTTCCCACC TGTCCACCAG TATCAGAGCT CTGACTTAGC CGTGCCGCCC 
GTGGCCAGTC TGCCTCCCAG CACCCTGGAC GGGCCTCTCT CATCTTCTCA GGAGAGCAGC 
TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG 
CCTTCGAGTG AGCACGCCTG TGAGGGGGAC TACGTCAGTC TGTATTGCTC TGGCCAGAGC 
AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG TCACCCGCGG CGAAGGATGG ACACTCCAGA 
GACGCCACAT CGGCCAGCTG TGTGCCCGGG AAGGAGGGCA GGGACAGCGG GGACAGGGCC 
CGGAAGTCAC CAGACGCTCC AGAGGCTCAG TCAGAGGAGG AGGTGGACGA GCTCTCCCTC 
ATCGACCACA ACGAGATCAT GGCGAGGCTG ACCCTCAAGC AAGAGGGCGA TGACGGGCCA 
GACGTCCGTG GAGGGTCGGG CGACATCCTC CTCGTCCATG CCACGGAGAC GGACCGGAAA 
GATCTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CACTCCTGAG 
GAGCTCATCC AGAAGCTGCA GTACAGATAT CCTTGCCGCC TCACCCTCCT TCACAGAGCC 
GTCTGCAGGG TGGGGTCAGC GATGAGGGGC AGTGCCCAGC CCAGCTCTGG TTCACTCAGG 
AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT TCCCTTCACG TCCCCACCCC TGCCCGCGAG 
GACGGCTGTT TCTTTAACTC GGGCTCCACA TACGAGAAGT TCTCACCTTT CGCCGACACA 
TTCAAGAAGC GTGTGAGCAA GAACACGTTC TTCGTGCTGG TGCGGGTGGT GGACGAGCTC 
TGCCTGGTAG AGCTGACAGA AGAGATCCTG AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG 
GTGCGCAGCG GGGAGCTCAG CCTAGCACGC GTGCTCCGCA AGAACATTCT GGACAAGGTC 
GACCAGAAGA AGCTGCTCCG GTGCGCCAAC TCTGACCAGC CCCTGGCGGC CCGGGGAGTG 
GCAGCCAGGC CCGGGACCTT GCACGACTTC CACAGCCATG AAATAGCCGA GCAGCTGACT 
CTGCTAGATG CCGAGCTCTT TTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCTAAA 
GAGCAGAACG AGGAGAAGAG CCCGAACCTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCGAT AATCATGTTG CAGGAAAAGG CCCAAGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTACGGAAGC TGAACAACTT CAACTCCTAC 
TTGGCCATCC TCTCGGCGCT GGACTCGGCC CCCATTCGCA GGCTGGAGTG GCAGAAACAG 
ACCTCAGAGG GCCTGGCCGA GTACTGCACG CTGATCGACA GCTCGTCATC CTTCCGCGCC 
TACCGGGCCG CCCTCTCGGA GGTAGAACCG CCCTGCATCC CATACCTGGG GCTCATCCTG 
CAGGACCTCA CCTTCGTCCA CCTGGGGAAC CCAGACTATA TTGACGGGAA GGTGAACTTC 
TCCAAACGCT GGCAGCAGTT CAACATCCTG GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TACGACATCC GGAGAAATGA CGACATCATT AACTTCTTCA ACGACTTCAG TGACCACCTG 
GCCGAGGAGG CCCTATGGGA ACTCTCTCTG AAAATCAAAC CCAGGAACAT AACGAGGAGG 
AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854464.1
CDS	301..3507
Translation	MEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYPCRLTLLHRAVCRVGSAMRGSAQPSSGSLRKGGGGGGGRPSLHVPTPAREDGCFFNSGSTYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998696.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998696.1

ORF Insert Sequence:

ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC TCGTACAAGT CCGTGTTTAG GTCCTACTCG 
CAGGATTTCG TGCCTCACAG CCAAGCTTCC GTCCAGCCTT TCCTTCCGTC TACCTCCTCT 
TCCTCTCCAC ACTTCCCACC TGTCCACCAG TATCAGAGCT CTGACTTAGC CGTGCCGCCC 
GTGGCCAGTC TGCCTCCCAG CACCCTGGAC GGGCCTCTCT CATCTTCTCA GGAGAGCAGC 
TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG 
CCTTCGAGTG AGCACGCCTG TGAGGGGGAC TACGTCAGTC TGTATTGCTC TGGCCAGAGC 
AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG TCACCCGCGG CGAAGGATGG ACACTCCAGA 
GACGCCACAT CGGCCAGCTG TGTGCCCGGG AAGGAGGGCA GGGACAGCGG GGACAGGGCC 
CGGAAGTCAC CAGACGCTCC AGAGGCTCAG TCAGAGGAGG AGGTGGACGA GCTCTCCCTC 
ATCGACCACA ACGAGATCAT GGCGAGGCTG ACCCTCAAGC AAGAGGGCGA TGACGGGCCA 
GACGTCCGTG GAGGGTCGGG CGACATCCTC CTCGTCCATG CCACGGAGAC GGACCGGAAA 
GATCTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CACTCCTGAG 
GAGCTCATCC AGAAGCTGCA GTACAGATAT CCTTGCCGCC TCACCCTCCT TCACAGAGCC 
GTCTGCAGGG TGGGGTCAGC GATGAGGGGC AGTGCCCAGC CCAGCTCTGG TTCACTCAGG 
AAGGGAGGAG GAGGAGGAGG AGGGCGGCCT TCCCTTCACG TCCCCACCCC TGCCCGCGAG 
GACGGCTGTT TCTTTAACTC GGGCTCCACA TACGAGAAGT TCTCACCTTT CGCCGACACA 
TTCAAGAAGC GTGTGAGCAA GAACACGTTC TTCGTGCTGG TGCGGGTGGT GGACGAGCTC 
TGCCTGGTAG AGCTGACAGA AGAGATCCTG AAGCTGCTGA TGGAGCTGGT CTTCCGCCTG 
GTGCGCAGCG GGGAGCTCAG CCTAGCACGC GTGCTCCGCA AGAACATTCT GGACAAGGTC 
GACCAGAAGA AGCTGCTCCG GTGCGCCAAC TCTGACCAGC CCCTGGCGGC CCGGGGAGTG 
GCAGCCAGGC CCGGGACCTT GCACGACTTC CACAGCCATG AAATAGCCGA GCAGCTGACT 
CTGCTAGATG CCGAGCTCTT TTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCTAAA 
GAGCAGAACG AGGAGAAGAG CCCGAACCTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCGAT AATCATGTTG CAGGAAAAGG CCCAAGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTACGGAAGC TGAACAACTT CAACTCCTAC 
TTGGCCATCC TCTCGGCGCT GGACTCGGCC CCCATTCGCA GGCTGGAGTG GCAGAAACAG 
ACCTCAGAGG GCCTGGCCGA GTACTGCACG CTGATCGACA GCTCGTCATC CTTCCGCGCC 
TACCGGGCCG CCCTCTCGGA GGTAGAACCG CCCTGCATCC CATACCTGGG GCTCATCCTG 
CAGGACCTCA CCTTCGTCCA CCTGGGGAAC CCAGACTATA TTGACGGGAA GGTGAACTTC 
TCCAAACGCT GGCAGCAGTT CAACATCCTG GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TACGACATCC GGAGAAATGA CGACATCATT AACTTCTTCA ACGACTTCAG TGACCACCTG 
GCCGAGGAGG CCCTATGGGA ACTCTCTCTG AAAATCAAAC CCAGGAACAT AACGAGGAGG 
AAGACAGATA GAGAAGAAAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998697.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854465.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OBa220365
Clone ID Related Accession (Same CDS sequence)	XM_024998697.1
Accession Version	XM_024998697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGGAGTCACC CGCGGCGAAG GATGGACACT CCAGAGACGC CACATCGGCC 
AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC AGCGGGGACA GGGCCCGGAA GTCACCAGAC 
GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG GACGAGCTCT CCCTCATCGA CCACAACGAG 
ATCATGGCGA GGCTGACCCT CAAGCAAGAG GGCGATGACG GGCCAGACGT CCGTGGAGGG 
TCGGGCGACA TCCTCCTCGT CCATGCCACG GAGACGGACC GGAAAGATCT GGTGTTGTAC 
TGCGAGGCCT TCCTGACCAC CTACAGGACC TTCATCACTC CTGAGGAGCT CATCCAGAAG 
CTGCAGTACA GATACGAGAA GTTCTCACCT TTCGCCGACA CATTCAAGAA GCGTGTGAGC 
AAGAACACGT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT AGAGCTGACA 
GAAGAGATCC TGAAGCTGCT GATGGAGCTG GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC 
AGCCTAGCAC GCGTGCTCCG CAAGAACATT CTGGACAAGG TCGACCAGAA GAAGCTGCTC 
CGGTGCGCCA ACTCTGACCA GCCCCTGGCG GCCCGGGGAG TGGCAGCCAG GCCCGGGACC 
TTGCACGACT TCCACAGCCA TGAAATAGCC GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC 
TTTTATAAAA TAGAGATTCC TGAGGTTTTG CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG 
AGCCCGAACC TGACCCAGTT CACGGAGCAC TTCAACAACA TGTCCTACTG GGTCCGGTCG 
ATAATCATGT TGCAGGAAAA GGCCCAAGAC AGGGAACGGC TGCTCTTGAA GTTCATCAAG 
ATCATGAAGC ACTTACGGAA GCTGAACAAC TTCAACTCCT ACTTGGCCAT CCTCTCGGCG 
CTGGACTCGG CCCCCATTCG CAGGCTGGAG TGGCAGAAAC AGACCTCAGA GGGCCTGGCC 
GAGTACTGCA CGCTGATCGA CAGCTCGTCA TCCTTCCGCG CCTACCGGGC CGCCCTCTCG 
GAGGTAGAAC CGCCCTGCAT CCCATACCTG GGGCTCATCC TGCAGGACCT CACCTTCGTC 
CACCTGGGGA ACCCAGACTA TATTGACGGG AAGGTGAACT TCTCCAAACG CTGGCAGCAG 
TTCAACATCC TGGACAGCAT GCGGTGCTTC CAGCAGGCGC ACTACGACAT CCGGAGAAAT 
GACGACATCA TTAACTTCTT CAACGACTTC AGTGACCACC TGGCCGAGGA GGCCCTATGG 
GAACTCTCTC TGAAAATCAA ACCCAGGAAC ATAACGAGGA GGAAGACAGA TAGAGAAGAA 
AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854465.1
CDS	188..3376
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998697.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998697.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGGAGTCACC CGCGGCGAAG GATGGACACT CCAGAGACGC CACATCGGCC 
AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC AGCGGGGACA GGGCCCGGAA GTCACCAGAC 
GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG GACGAGCTCT CCCTCATCGA CCACAACGAG 
ATCATGGCGA GGCTGACCCT CAAGCAAGAG GGCGATGACG GGCCAGACGT CCGTGGAGGG 
TCGGGCGACA TCCTCCTCGT CCATGCCACG GAGACGGACC GGAAAGATCT GGTGTTGTAC 
TGCGAGGCCT TCCTGACCAC CTACAGGACC TTCATCACTC CTGAGGAGCT CATCCAGAAG 
CTGCAGTACA GATACGAGAA GTTCTCACCT TTCGCCGACA CATTCAAGAA GCGTGTGAGC 
AAGAACACGT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT AGAGCTGACA 
GAAGAGATCC TGAAGCTGCT GATGGAGCTG GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC 
AGCCTAGCAC GCGTGCTCCG CAAGAACATT CTGGACAAGG TCGACCAGAA GAAGCTGCTC 
CGGTGCGCCA ACTCTGACCA GCCCCTGGCG GCCCGGGGAG TGGCAGCCAG GCCCGGGACC 
TTGCACGACT TCCACAGCCA TGAAATAGCC GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC 
TTTTATAAAA TAGAGATTCC TGAGGTTTTG CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG 
AGCCCGAACC TGACCCAGTT CACGGAGCAC TTCAACAACA TGTCCTACTG GGTCCGGTCG 
ATAATCATGT TGCAGGAAAA GGCCCAAGAC AGGGAACGGC TGCTCTTGAA GTTCATCAAG 
ATCATGAAGC ACTTACGGAA GCTGAACAAC TTCAACTCCT ACTTGGCCAT CCTCTCGGCG 
CTGGACTCGG CCCCCATTCG CAGGCTGGAG TGGCAGAAAC AGACCTCAGA GGGCCTGGCC 
GAGTACTGCA CGCTGATCGA CAGCTCGTCA TCCTTCCGCG CCTACCGGGC CGCCCTCTCG 
GAGGTAGAAC CGCCCTGCAT CCCATACCTG GGGCTCATCC TGCAGGACCT CACCTTCGTC 
CACCTGGGGA ACCCAGACTA TATTGACGGG AAGGTGAACT TCTCCAAACG CTGGCAGCAG 
TTCAACATCC TGGACAGCAT GCGGTGCTTC CAGCAGGCGC ACTACGACAT CCGGAGAAAT 
GACGACATCA TTAACTTCTT CAACGACTTC AGTGACCACC TGGCCGAGGA GGCCCTATGG 
GAACTCTCTC TGAAAATCAA ACCCAGGAAC ATAACGAGGA GGAAGACAGA TAGAGAAGAA 
AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024998698.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854466.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OBa220366
Clone ID Related Accession (Same CDS sequence)	XM_024998698.1
Accession Version	XM_024998698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCT TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCTATTGA TAAGAAGGTC 
CTGGAGATGC TCCCAGGGTC CGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AGCGACCCTC GAATTCAGCA CAGCTCAGCC CTCTCCTCCT GCTACAGCCG AGTGTACCAA 
AGTCTCGCCA ATCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GATAAAGAGA TGGTGACAAC TGTGAAGGGG GTCATCAAGG CCGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GACTCACCAT CGAGAAGCAG GGGCATCCAT CTCCAACAAG CCCTGTGAAA 
CCCAGCTCCC CAGCCTGCAA ACCTGACAGC CAGTCTGAGC TCCCCCTGAC GGATCGAGAG 
ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGGAGTCA 
CCCGCGGCGA AGGATGGACA CTCCAGAGAC GCCACATCGG CCAGCTGTGT GCCCGGGAAG 
GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG AAGTCACCAG ACGCTCCAGA GGCTCAGTCA 
GAGGAGGAGG TGGACGAGCT CTCCCTCATC GACCACAACG AGATCATGGC GAGGCTGACC 
CTCAAGCAAG AGGGCGATGA CGGGCCAGAC GTCCGTGGAG GGTCGGGCGA CATCCTCCTC 
GTCCATGCCA CGGAGACGGA CCGGAAAGAT CTGGTGTTGT ACTGCGAGGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAC TCCTGAGGAG CTCATCCAGA AGCTGCAGTA CAGATACGAG 
AAGTTCTCAC CTTTCGCCGA CACATTCAAG AAGCGTGTGA GCAAGAACAC GTTCTTCGTG 
CTGGTGCGGG TGGTGGACGA GCTCTGCCTG GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTGGTGCGC AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC 
CGCAAGAACA TTCTGGACAA GGTCGACCAG AAGAAGCTGC TCCGGTGCGC CAACTCTGAC 
CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC AGGCCCGGGA CCTTGCACGA CTTCCACAGC 
CATGAAATAG CCGAGCAGCT GACTCTGCTA GATGCCGAGC TCTTTTATAA AATAGAGATT 
CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG AACGAGGAGA AGAGCCCGAA CCTGACCCAG 
TTCACGGAGC ACTTCAACAA CATGTCCTAC TGGGTCCGGT CGATAATCAT GTTGCAGGAA 
AAGGCCCAAG ACAGGGAACG GCTGCTCTTG AAGTTCATCA AGATCATGAA GCACTTACGG 
AAGCTGAACA ACTTCAACTC CTACTTGGCC ATCCTCTCGG CGCTGGACTC GGCCCCCATT 
CGCAGGCTGG AGTGGCAGAA ACAGACCTCA GAGGGCCTGG CCGAGTACTG CACGCTGATC 
GACAGCTCGT CATCCTTCCG CGCCTACCGG GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC 
ATCCCATACC TGGGGCTCAT CCTGCAGGAC CTCACCTTCG TCCACCTGGG GAACCCAGAC 
TATATTGACG GGAAGGTGAA CTTCTCCAAA CGCTGGCAGC AGTTCAACAT CCTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTACGAC ATCCGGAGAA ATGACGACAT CATTAACTTC 
TTCAACGACT TCAGTGACCA CCTGGCCGAG GAGGCCCTAT GGGAACTCTC TCTGAAAATC 
AAACCCAGGA ACATAACGAG GAGGAAGACA GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854466.1
CDS	335..3505
Translation	MGNAVEKQKPLKRSHLCPWKQDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024998698.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998698.1

ORF Insert Sequence:

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCT TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCTATTGA TAAGAAGGTC 
CTGGAGATGC TCCCAGGGTC CGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AGCGACCCTC GAATTCAGCA CAGCTCAGCC CTCTCCTCCT GCTACAGCCG AGTGTACCAA 
AGTCTCGCCA ATCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GATAAAGAGA TGGTGACAAC TGTGAAGGGG GTCATCAAGG CCGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GACTCACCAT CGAGAAGCAG GGGCATCCAT CTCCAACAAG CCCTGTGAAA 
CCCAGCTCCC CAGCCTGCAA ACCTGACAGC CAGTCTGAGC TCCCCCTGAC GGATCGAGAG 
ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGGAGTCA 
CCCGCGGCGA AGGATGGACA CTCCAGAGAC GCCACATCGG CCAGCTGTGT GCCCGGGAAG 
GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG AAGTCACCAG ACGCTCCAGA GGCTCAGTCA 
GAGGAGGAGG TGGACGAGCT CTCCCTCATC GACCACAACG AGATCATGGC GAGGCTGACC 
CTCAAGCAAG AGGGCGATGA CGGGCCAGAC GTCCGTGGAG GGTCGGGCGA CATCCTCCTC 
GTCCATGCCA CGGAGACGGA CCGGAAAGAT CTGGTGTTGT ACTGCGAGGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAC TCCTGAGGAG CTCATCCAGA AGCTGCAGTA CAGATACGAG 
AAGTTCTCAC CTTTCGCCGA CACATTCAAG AAGCGTGTGA GCAAGAACAC GTTCTTCGTG 
CTGGTGCGGG TGGTGGACGA GCTCTGCCTG GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTGGTGCGC AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC 
CGCAAGAACA TTCTGGACAA GGTCGACCAG AAGAAGCTGC TCCGGTGCGC CAACTCTGAC 
CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC AGGCCCGGGA CCTTGCACGA CTTCCACAGC 
CATGAAATAG CCGAGCAGCT GACTCTGCTA GATGCCGAGC TCTTTTATAA AATAGAGATT 
CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG AACGAGGAGA AGAGCCCGAA CCTGACCCAG 
TTCACGGAGC ACTTCAACAA CATGTCCTAC TGGGTCCGGT CGATAATCAT GTTGCAGGAA 
AAGGCCCAAG ACAGGGAACG GCTGCTCTTG AAGTTCATCA AGATCATGAA GCACTTACGG 
AAGCTGAACA ACTTCAACTC CTACTTGGCC ATCCTCTCGG CGCTGGACTC GGCCCCCATT 
CGCAGGCTGG AGTGGCAGAA ACAGACCTCA GAGGGCCTGG CCGAGTACTG CACGCTGATC 
GACAGCTCGT CATCCTTCCG CGCCTACCGG GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC 
ATCCCATACC TGGGGCTCAT CCTGCAGGAC CTCACCTTCG TCCACCTGGG GAACCCAGAC 
TATATTGACG GGAAGGTGAA CTTCTCCAAA CGCTGGCAGC AGTTCAACAT CCTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTACGAC ATCCGGAGAA ATGACGACAT CATTAACTTC 
TTCAACGACT TCAGTGACCA CCTGGCCGAG GAGGCCCTAT GGGAACTCTC TCTGAAAATC 
AAACCCAGGA ACATAACGAG GAGGAAGACA GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473625.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329111.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

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CloneID	OBa91979
Clone ID Related Accession (Same CDS sequence)	XM_015473625.2 , XM_015473625.1
Accession Version	XM_015473625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATACG AGAAGTTCTC ACCTTTCGCC 
GACACATTCA AGAAGCGTGT GAGCAAGAAC ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC 
GAGCTCTGCC TGGTAGAGCT GACAGAAGAG ATCCTGAAGC TGCTGATGGA GCTGGTCTTC 
CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA GCACGCGTGC TCCGCAAGAA CATTCTGGAC 
AAGGTCGACC AGAAGAAGCT GCTCCGGTGC GCCAACTCTG ACCAGCCCCT GGCGGCCCGG 
GGAGTGGCAG CCAGGCCCGG GACCTTGCAC GACTTCCACA GCCATGAAAT AGCCGAGCAG 
CTGACTCTGC TAGATGCCGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCTAAAGAGC AGAACGAGGA GAAGAGCCCG AACCTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCGATAATC ATGTTGCAGG AAAAGGCCCA AGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTCAAC 
TCCTACTTGG CCATCCTCTC GGCGCTGGAC TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACGCTGA TCGACAGCTC GTCATCCTTC 
CGCGCCTACC GGGCCGCCCT CTCGGAGGTA GAACCGCCCT GCATCCCATA CCTGGGGCTC 
ATCCTGCAGG ACCTCACCTT CGTCCACCTG GGGAACCCAG ACTATATTGA CGGGAAGGTG 
AACTTCTCCA AACGCTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGGTG CTTCCAGCAG 
GCGCACTACG ACATCCGGAG AAATGACGAC ATCATTAACT TCTTCAACGA CTTCAGTGAC 
CACCTGGCCG AGGAGGCCCT ATGGGAACTC TCTCTGAAAA TCAAACCCAG GAACATAACG 
AGGAGGAAGA CAGATAGAGA AGAAAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329111.1
CDS	123..3935
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473625.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473625.2

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATACG AGAAGTTCTC ACCTTTCGCC 
GACACATTCA AGAAGCGTGT GAGCAAGAAC ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC 
GAGCTCTGCC TGGTAGAGCT GACAGAAGAG ATCCTGAAGC TGCTGATGGA GCTGGTCTTC 
CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA GCACGCGTGC TCCGCAAGAA CATTCTGGAC 
AAGGTCGACC AGAAGAAGCT GCTCCGGTGC GCCAACTCTG ACCAGCCCCT GGCGGCCCGG 
GGAGTGGCAG CCAGGCCCGG GACCTTGCAC GACTTCCACA GCCATGAAAT AGCCGAGCAG 
CTGACTCTGC TAGATGCCGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCTAAAGAGC AGAACGAGGA GAAGAGCCCG AACCTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCGATAATC ATGTTGCAGG AAAAGGCCCA AGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTCAAC 
TCCTACTTGG CCATCCTCTC GGCGCTGGAC TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACGCTGA TCGACAGCTC GTCATCCTTC 
CGCGCCTACC GGGCCGCCCT CTCGGAGGTA GAACCGCCCT GCATCCCATA CCTGGGGCTC 
ATCCTGCAGG ACCTCACCTT CGTCCACCTG GGGAACCCAG ACTATATTGA CGGGAAGGTG 
AACTTCTCCA AACGCTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGGTG CTTCCAGCAG 
GCGCACTACG ACATCCGGAG AAATGACGAC ATCATTAACT TCTTCAACGA CTTCAGTGAC 
CACCTGGCCG AGGAGGCCCT ATGGGAACTC TCTCTGAAAA TCAAACCCAG GAACATAACG 
AGGAGGAAGA CAGATAGAGA AGAAAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473625.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329111.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91979
Clone ID Related Accession (Same CDS sequence)	XM_015473625.2 , XM_015473625.1
Accession Version	XM_015473625.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATACG AGAAGTTCTC ACCTTTCGCC 
GACACATTCA AGAAGCGTGT GAGCAAGAAC ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC 
GAGCTCTGCC TGGTAGAGCT GACAGAAGAG ATCCTGAAGC TGCTGATGGA GCTGGTCTTC 
CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA GCACGCGTGC TCCGCAAGAA CATTCTGGAC 
AAGGTCGACC AGAAGAAGCT GCTCCGGTGC GCCAACTCTG ACCAGCCCCT GGCGGCCCGG 
GGAGTGGCAG CCAGGCCCGG GACCTTGCAC GACTTCCACA GCCATGAAAT AGCCGAGCAG 
CTGACTCTGC TAGATGCCGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCTAAAGAGC AGAACGAGGA GAAGAGCCCG AACCTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCGATAATC ATGTTGCAGG AAAAGGCCCA AGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTCAAC 
TCCTACTTGG CCATCCTCTC GGCGCTGGAC TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACGCTGA TCGACAGCTC GTCATCCTTC 
CGCGCCTACC GGGCCGCCCT CTCGGAGGTA GAACCGCCCT GCATCCCATA CCTGGGGCTC 
ATCCTGCAGG ACCTCACCTT CGTCCACCTG GGGAACCCAG ACTATATTGA CGGGAAGGTG 
AACTTCTCCA AACGCTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGGTG CTTCCAGCAG 
GCGCACTACG ACATCCGGAG AAATGACGAC ATCATTAACT TCTTCAACGA CTTCAGTGAC 
CACCTGGCCG AGGAGGCCCT ATGGGAACTC TCTCTGAAAA TCAAACCCAG GAACATAACG 
AGGAGGAAGA CAGATAGAGA AGAAAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329111.1
CDS	541..4353
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473625.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473625.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGAGTGA GCACGCCTGT GAGGGGGACT ACGTCAGTCT GTATTGCTCT 
GGCCAGAGCA GCGAGGAGCT GGCCCGCCCT CGAGGAGAGT CACCCGCGGC GAAGGATGGA 
CACTCCAGAG ACGCCACATC GGCCAGCTGT GTGCCCGGGA AGGAGGGCAG GGACAGCGGG 
GACAGGGCCC GGAAGTCACC AGACGCTCCA GAGGCTCAGT CAGAGGAGGA GGTGGACGAG 
CTCTCCCTCA TCGACCACAA CGAGATCATG GCGAGGCTGA CCCTCAAGCA AGAGGGCGAT 
GACGGGCCAG ACGTCCGTGG AGGGTCGGGC GACATCCTCC TCGTCCATGC CACGGAGACG 
GACCGGAAAG ATCTGGTGTT GTACTGCGAG GCCTTCCTGA CCACCTACAG GACCTTCATC 
ACTCCTGAGG AGCTCATCCA GAAGCTGCAG TACAGATACG AGAAGTTCTC ACCTTTCGCC 
GACACATTCA AGAAGCGTGT GAGCAAGAAC ACGTTCTTCG TGCTGGTGCG GGTGGTGGAC 
GAGCTCTGCC TGGTAGAGCT GACAGAAGAG ATCCTGAAGC TGCTGATGGA GCTGGTCTTC 
CGCCTGGTGC GCAGCGGGGA GCTCAGCCTA GCACGCGTGC TCCGCAAGAA CATTCTGGAC 
AAGGTCGACC AGAAGAAGCT GCTCCGGTGC GCCAACTCTG ACCAGCCCCT GGCGGCCCGG 
GGAGTGGCAG CCAGGCCCGG GACCTTGCAC GACTTCCACA GCCATGAAAT AGCCGAGCAG 
CTGACTCTGC TAGATGCCGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCTAAAGAGC AGAACGAGGA GAAGAGCCCG AACCTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCGATAATC ATGTTGCAGG AAAAGGCCCA AGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTCAAC 
TCCTACTTGG CCATCCTCTC GGCGCTGGAC TCGGCCCCCA TTCGCAGGCT GGAGTGGCAG 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACGCTGA TCGACAGCTC GTCATCCTTC 
CGCGCCTACC GGGCCGCCCT CTCGGAGGTA GAACCGCCCT GCATCCCATA CCTGGGGCTC 
ATCCTGCAGG ACCTCACCTT CGTCCACCTG GGGAACCCAG ACTATATTGA CGGGAAGGTG 
AACTTCTCCA AACGCTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGGTG CTTCCAGCAG 
GCGCACTACG ACATCCGGAG AAATGACGAC ATCATTAACT TCTTCAACGA CTTCAGTGAC 
CACCTGGCCG AGGAGGCCCT ATGGGAACTC TCTCTGAAAA TCAAACCCAG GAACATAACG 
AGGAGGAAGA CAGATAGAGA AGAAAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473626.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329112.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91980
Clone ID Related Accession (Same CDS sequence)	XM_015473626.1
Accession Version	XM_015473626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGCCTCCT GTGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC 
TCCTTTCTCA GCCGGCACGG CAGCTTACCT GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC 
TACTCGCAGG ATTTCGTGCC TCACAGCCAA GCTTCCGTCC AGCCTTTCCT TCCGTCTACC 
TCCTCTTCCT CTCCACACTT CCCACCTGTC CACCAGTATC AGAGCTCTGA CTTAGCCGTG 
CCGCCCGTGG CCAGTCTGCC TCCCAGCACC CTGGACGGGC CTCTCTCATC TTCTCAGGAG 
AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC 
CAGACGCCTT CGAGTGAGCA CGCCTGTGAG GGGGACTACG TCAGTCTGTA TTGCTCTGGC 
CAGAGCAGCG AGGAGCTGGC CCGCCCTCGA GGAGAGTCAC CCGCGGCGAA GGATGGACAC 
TCCAGAGACG CCACATCGGC CAGCTGTGTG CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC 
AGGGCCCGGA AGTCACCAGA CGCTCCAGAG GCTCAGTCAG AGGAGGAGGT GGACGAGCTC 
TCCCTCATCG ACCACAACGA GATCATGGCG AGGCTGACCC TCAAGCAAGA GGGCGATGAC 
GGGCCAGACG TCCGTGGAGG GTCGGGCGAC ATCCTCCTCG TCCATGCCAC GGAGACGGAC 
CGGAAAGATC TGGTGTTGTA CTGCGAGGCC TTCCTGACCA CCTACAGGAC CTTCATCACT 
CCTGAGGAGC TCATCCAGAA GCTGCAGTAC AGATACGAGA AGTTCTCACC TTTCGCCGAC 
ACATTCAAGA AGCGTGTGAG CAAGAACACG TTCTTCGTGC TGGTGCGGGT GGTGGACGAG 
CTCTGCCTGG TAGAGCTGAC AGAAGAGATC CTGAAGCTGC TGATGGAGCT GGTCTTCCGC 
CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA CGCGTGCTCC GCAAGAACAT TCTGGACAAG 
GTCGACCAGA AGAAGCTGCT CCGGTGCGCC AACTCTGACC AGCCCCTGGC GGCCCGGGGA 
GTGGCAGCCA GGCCCGGGAC CTTGCACGAC TTCCACAGCC ATGAAATAGC CGAGCAGCTG 
ACTCTGCTAG ATGCCGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCT 
AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC CTGACCCAGT TCACGGAGCA CTTCAACAAC 
ATGTCCTACT GGGTCCGGTC GATAATCATG TTGCAGGAAA AGGCCCAAGA CAGGGAACGG 
CTGCTCTTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTCAACTCC 
TACTTGGCCA TCCTCTCGGC GCTGGACTCG GCCCCCATTC GCAGGCTGGA GTGGCAGAAA 
CAGACCTCAG AGGGCCTGGC CGAGTACTGC ACGCTGATCG ACAGCTCGTC ATCCTTCCGC 
GCCTACCGGG CCGCCCTCTC GGAGGTAGAA CCGCCCTGCA TCCCATACCT GGGGCTCATC 
CTGCAGGACC TCACCTTCGT CCACCTGGGG AACCCAGACT ATATTGACGG GAAGGTGAAC 
TTCTCCAAAC GCTGGCAGCA GTTCAACATC CTGGACAGCA TGCGGTGCTT CCAGCAGGCG 
CACTACGACA TCCGGAGAAA TGACGACATC ATTAACTTCT TCAACGACTT CAGTGACCAC 
CTGGCCGAGG AGGCCCTATG GGAACTCTCT CTGAAAATCA AACCCAGGAA CATAACGAGG 
AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329112.1
CDS	541..4350
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473626.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473626.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGCCTCCT GTGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC 
TCCTTTCTCA GCCGGCACGG CAGCTTACCT GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC 
TACTCGCAGG ATTTCGTGCC TCACAGCCAA GCTTCCGTCC AGCCTTTCCT TCCGTCTACC 
TCCTCTTCCT CTCCACACTT CCCACCTGTC CACCAGTATC AGAGCTCTGA CTTAGCCGTG 
CCGCCCGTGG CCAGTCTGCC TCCCAGCACC CTGGACGGGC CTCTCTCATC TTCTCAGGAG 
AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC 
CAGACGCCTT CGAGTGAGCA CGCCTGTGAG GGGGACTACG TCAGTCTGTA TTGCTCTGGC 
CAGAGCAGCG AGGAGCTGGC CCGCCCTCGA GGAGAGTCAC CCGCGGCGAA GGATGGACAC 
TCCAGAGACG CCACATCGGC CAGCTGTGTG CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC 
AGGGCCCGGA AGTCACCAGA CGCTCCAGAG GCTCAGTCAG AGGAGGAGGT GGACGAGCTC 
TCCCTCATCG ACCACAACGA GATCATGGCG AGGCTGACCC TCAAGCAAGA GGGCGATGAC 
GGGCCAGACG TCCGTGGAGG GTCGGGCGAC ATCCTCCTCG TCCATGCCAC GGAGACGGAC 
CGGAAAGATC TGGTGTTGTA CTGCGAGGCC TTCCTGACCA CCTACAGGAC CTTCATCACT 
CCTGAGGAGC TCATCCAGAA GCTGCAGTAC AGATACGAGA AGTTCTCACC TTTCGCCGAC 
ACATTCAAGA AGCGTGTGAG CAAGAACACG TTCTTCGTGC TGGTGCGGGT GGTGGACGAG 
CTCTGCCTGG TAGAGCTGAC AGAAGAGATC CTGAAGCTGC TGATGGAGCT GGTCTTCCGC 
CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA CGCGTGCTCC GCAAGAACAT TCTGGACAAG 
GTCGACCAGA AGAAGCTGCT CCGGTGCGCC AACTCTGACC AGCCCCTGGC GGCCCGGGGA 
GTGGCAGCCA GGCCCGGGAC CTTGCACGAC TTCCACAGCC ATGAAATAGC CGAGCAGCTG 
ACTCTGCTAG ATGCCGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCT 
AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC CTGACCCAGT TCACGGAGCA CTTCAACAAC 
ATGTCCTACT GGGTCCGGTC GATAATCATG TTGCAGGAAA AGGCCCAAGA CAGGGAACGG 
CTGCTCTTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTCAACTCC 
TACTTGGCCA TCCTCTCGGC GCTGGACTCG GCCCCCATTC GCAGGCTGGA GTGGCAGAAA 
CAGACCTCAG AGGGCCTGGC CGAGTACTGC ACGCTGATCG ACAGCTCGTC ATCCTTCCGC 
GCCTACCGGG CCGCCCTCTC GGAGGTAGAA CCGCCCTGCA TCCCATACCT GGGGCTCATC 
CTGCAGGACC TCACCTTCGT CCACCTGGGG AACCCAGACT ATATTGACGG GAAGGTGAAC 
TTCTCCAAAC GCTGGCAGCA GTTCAACATC CTGGACAGCA TGCGGTGCTT CCAGCAGGCG 
CACTACGACA TCCGGAGAAA TGACGACATC ATTAACTTCT TCAACGACTT CAGTGACCAC 
CTGGCCGAGG AGGCCCTATG GGAACTCTCT CTGAAAATCA AACCCAGGAA CATAACGAGG 
AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473627.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329113.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

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CloneID	OBa91981
Clone ID Related Accession (Same CDS sequence)	XM_015473627.1
Accession Version	XM_015473627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGG TGAGGCCACT GGATGCATGG ATTTCATTCC TTCTCTACTC TCAGCGTTCT 
CATCTCTCCT CCTTCACCAT GAAGCTGAAG GACAAATTCC ACTCGCCCAA AATCAAGAGG 
ACGCCATCAA AGAAGGGAAA ACCAGCCGAG GTGTCTGTGA AGATTCCAGA GAAGCCCGTG 
AACAAAGAGG CAACAGACAG ATTTCTACCA GAGGGCTACC CTATCCCCTT GGATCTGGAG 
CAGCAGGCAG TAGAGTTTAT GTCCACCAGT GCTGTGGCTT CCAGGTCTCA AAGGCAGAAG 
AACCTGAGCT GGCTGGAGGA GAAAGAGAAG GAAGTTGTCA GTGCCTTGCG CTACTTTAAG 
ACCATTGTGG ACAAAATGGC TATTGATAAG AAGGTCCTGG AGATGCTCCC AGGGTCCGCC 
AGCAAGGTGC TGGAGGCCAT CTTACCCCTG GTGCAGAGCG ACCCTCGAAT TCAGCACAGC 
TCAGCCCTCT CCTCCTGCTA CAGCCGAGTG TACCAAAGTC TCGCCAATCT CATCCGTTGG 
TCTGACCAAG TGATGCTGGA AGGCGTGAAC TCAGAAGATA AAGAGATGGT GACAACTGTG 
AAGGGGGTCA TCAAGGCCGT GCTGGATGGA GTGAAGGAGC TGGTCAGACT CACCATCGAG 
AAGCAGGGGC ATCCATCTCC AACAAGCCCT GTGAAACCCA GCTCCCCAGC CTGCAAACCT 
GACAGCCAGT CTGAGCTCCC CCTGACGGAT CGAGAGATGG AGATACTAAA CAAGACGACC 
AGCCTGTCAC AGTCAACCGA GGGGCTCCAA GACTCTATGG ACGAAGAGGT TGCACCCCCC 
AAGCCCCCCT TACCTGGCAT TAGGGTGGTT GATAACAGTC CTCCACCAGC ACTGCCCCCG 
AAGAAGAGAC AGTCGGCTCC GTCCCCCACG CGTGTGGCCG TTGTGGCCCC CATGAGTCGA 
GCCACCAGTG GCTCCAGTTT GCCTGTTGGA ATTAATAGGC AGGACTTTGA CGTGGACTGT 
TACGCACAGA GACGGCTGTC GGGAGGCAGC CACTCGTACA GCGGTGAGTC TCCCCGCCTG 
TCGCCCTGCA GCAGCATCGG CAAGCTCAGC AAGTCGGACG AGCAGCTGTC CTCTCTGGAC 
AGGGACAGCG GGCAGTGCTC CCGGAACACA AGCTGTGAAA CACTAGATCA TTACGATCCT 
GACTACGAGT TCCTTCAGCA AGACCTCTCC AACGCAGACC AGATACCTCA GCAAGTGGCC 
TGTAACCTCA GCCCGCTGCC AGAGTCCTTG GGGGAGTCTG GGTCTCCCTT TCTTGGCCAT 
CCTCTCCAGT TGCCTCTGGG CAGCTGTCCC CAGCCAGATG GGTCCTCAGC CCCGGGGCAG 
CAGACGGACG TGCCGCCAGC CCTGCCCGAG AAGAAGCGCA GGAGTGCAGC CTCCCAGACC 
TTGGACAGCT CTGGCTGCCG GGCGTCCTAC GAGCGACACC CCTCACAGTA CGACAACATC 
TCCGAGGATG ACCTGCAGAA CCCAGCCTCC GTCCAGTCCG TCCCCTTCAC GCCCTTTGCT 
GCTATTCTTC CCTTTCAGCA AGGAGGTTCC TCAGCCTCTG TCGACTTCAT GGGTGATTTC 
ACTGTTTCTG AATCAACGGG TGACCCAGAA AAACCGCCAC CTCTCCCCGA GAAGAAAAAC 
AAACACATGC TGGCCTACAT GCAGCTGCTT GAGGACTACT CGGAGCCACA GCCTTCCATG 
TTCTACCAGA CGCCGCAGAA GGAACACGTG TACCAGCAGA AGAACAAGCT TCTCATGGAG 
GTGTATGGCT TCAACGACTC CTTCAGCACC GGCGACGCCG CACACGATCT CGCCCCGCCC 
CCCGCCCTGC CCCCCAAGCA GCGGCAGCTG CAGGCCTCCT GTGCTGCTTC TTCCTTCTCC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC TCCTTTCTCA GCCGGCACGG CAGCTTACCT 
GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC TACTCGCAGG ATTTCGTGCC TCACAGCCAA 
GCTTCCGTCC AGCCTTTCCT TCCGTCTACC TCCTCTTCCT CTCCACACTT CCCACCTGTC 
CACCAGTATC AGAGCTCTGA CTTAGCCGTG CCGCCCGTGG CCAGTCTGCC TCCCAGCACC 
CTGGACGGGC CTCTCTCATC TTCTCAGGAG AGCAGCTTTC ACGGGAACAC TGTCTGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGCCC CAGACGCCTT CGAGTGAGCA CGCCTGTGAG 
GGGGACTACG TCAGTCTGTA TTGCTCTGGC CAGAGCAGCG AGGAGCTGGC CCGCCCTCGA 
GGAGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329113.1
CDS	16..3795
Translation	MWWVRPLDAWISFLLYSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473627.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473627.1

ORF Insert Sequence:

ATGTGGTGGG TGAGGCCACT GGATGCATGG ATTTCATTCC TTCTCTACTC TCAGCGTTCT 
CATCTCTCCT CCTTCACCAT GAAGCTGAAG GACAAATTCC ACTCGCCCAA AATCAAGAGG 
ACGCCATCAA AGAAGGGAAA ACCAGCCGAG GTGTCTGTGA AGATTCCAGA GAAGCCCGTG 
AACAAAGAGG CAACAGACAG ATTTCTACCA GAGGGCTACC CTATCCCCTT GGATCTGGAG 
CAGCAGGCAG TAGAGTTTAT GTCCACCAGT GCTGTGGCTT CCAGGTCTCA AAGGCAGAAG 
AACCTGAGCT GGCTGGAGGA GAAAGAGAAG GAAGTTGTCA GTGCCTTGCG CTACTTTAAG 
ACCATTGTGG ACAAAATGGC TATTGATAAG AAGGTCCTGG AGATGCTCCC AGGGTCCGCC 
AGCAAGGTGC TGGAGGCCAT CTTACCCCTG GTGCAGAGCG ACCCTCGAAT TCAGCACAGC 
TCAGCCCTCT CCTCCTGCTA CAGCCGAGTG TACCAAAGTC TCGCCAATCT CATCCGTTGG 
TCTGACCAAG TGATGCTGGA AGGCGTGAAC TCAGAAGATA AAGAGATGGT GACAACTGTG 
AAGGGGGTCA TCAAGGCCGT GCTGGATGGA GTGAAGGAGC TGGTCAGACT CACCATCGAG 
AAGCAGGGGC ATCCATCTCC AACAAGCCCT GTGAAACCCA GCTCCCCAGC CTGCAAACCT 
GACAGCCAGT CTGAGCTCCC CCTGACGGAT CGAGAGATGG AGATACTAAA CAAGACGACC 
AGCCTGTCAC AGTCAACCGA GGGGCTCCAA GACTCTATGG ACGAAGAGGT TGCACCCCCC 
AAGCCCCCCT TACCTGGCAT TAGGGTGGTT GATAACAGTC CTCCACCAGC ACTGCCCCCG 
AAGAAGAGAC AGTCGGCTCC GTCCCCCACG CGTGTGGCCG TTGTGGCCCC CATGAGTCGA 
GCCACCAGTG GCTCCAGTTT GCCTGTTGGA ATTAATAGGC AGGACTTTGA CGTGGACTGT 
TACGCACAGA GACGGCTGTC GGGAGGCAGC CACTCGTACA GCGGTGAGTC TCCCCGCCTG 
TCGCCCTGCA GCAGCATCGG CAAGCTCAGC AAGTCGGACG AGCAGCTGTC CTCTCTGGAC 
AGGGACAGCG GGCAGTGCTC CCGGAACACA AGCTGTGAAA CACTAGATCA TTACGATCCT 
GACTACGAGT TCCTTCAGCA AGACCTCTCC AACGCAGACC AGATACCTCA GCAAGTGGCC 
TGTAACCTCA GCCCGCTGCC AGAGTCCTTG GGGGAGTCTG GGTCTCCCTT TCTTGGCCAT 
CCTCTCCAGT TGCCTCTGGG CAGCTGTCCC CAGCCAGATG GGTCCTCAGC CCCGGGGCAG 
CAGACGGACG TGCCGCCAGC CCTGCCCGAG AAGAAGCGCA GGAGTGCAGC CTCCCAGACC 
TTGGACAGCT CTGGCTGCCG GGCGTCCTAC GAGCGACACC CCTCACAGTA CGACAACATC 
TCCGAGGATG ACCTGCAGAA CCCAGCCTCC GTCCAGTCCG TCCCCTTCAC GCCCTTTGCT 
GCTATTCTTC CCTTTCAGCA AGGAGGTTCC TCAGCCTCTG TCGACTTCAT GGGTGATTTC 
ACTGTTTCTG AATCAACGGG TGACCCAGAA AAACCGCCAC CTCTCCCCGA GAAGAAAAAC 
AAACACATGC TGGCCTACAT GCAGCTGCTT GAGGACTACT CGGAGCCACA GCCTTCCATG 
TTCTACCAGA CGCCGCAGAA GGAACACGTG TACCAGCAGA AGAACAAGCT TCTCATGGAG 
GTGTATGGCT TCAACGACTC CTTCAGCACC GGCGACGCCG CACACGATCT CGCCCCGCCC 
CCCGCCCTGC CCCCCAAGCA GCGGCAGCTG CAGGCCTCCT GTGCTGCTTC TTCCTTCTCC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCA TCAGTGTGTC CGTCTCTAAC TCCTTTCTCA GCCGGCACGG CAGCTTACCT 
GTGCCCTCGT ACAAGTCCGT GTTTAGGTCC TACTCGCAGG ATTTCGTGCC TCACAGCCAA 
GCTTCCGTCC AGCCTTTCCT TCCGTCTACC TCCTCTTCCT CTCCACACTT CCCACCTGTC 
CACCAGTATC AGAGCTCTGA CTTAGCCGTG CCGCCCGTGG CCAGTCTGCC TCCCAGCACC 
CTGGACGGGC CTCTCTCATC TTCTCAGGAG AGCAGCTTTC ACGGGAACAC TGTCTGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGCCC CAGACGCCTT CGAGTGAGCA CGCCTGTGAG 
GGGGACTACG TCAGTCTGTA TTGCTCTGGC CAGAGCAGCG AGGAGCTGGC CCGCCCTCGA 
GGAGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473628.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329114.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa91982
Clone ID Related Accession (Same CDS sequence)	XM_015473628.1 , XM_015473628.2
Accession Version	XM_015473628.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329114.1
CDS	536..4267
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473628.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473628.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473628.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329114.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa91982
Clone ID Related Accession (Same CDS sequence)	XM_015473628.1 , XM_015473628.2
Accession Version	XM_015473628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329114.1
CDS	121..3852
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473628.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473628.2

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGTACAAGTC CGTGTTTAGG 
TCCTACTCGC AGGATTTCGT GCCTCACAGC CAAGCTTCCG TCCAGCCTTT CCTTCCGTCT 
ACCTCCTCTT CCTCTCCACA CTTCCCACCT GTCCACCAGT ATCAGAGCTC TGACTTAGCC 
GTGCCGCCCG TGGCCAGTCT GCCTCCCAGC ACCCTGGACG GGCCTCTCTC ATCTTCTCAG 
GAGAGCAGCT TTCACGGGAA CACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
CCCCAGACGC CTTCGGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473632.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329118.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

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CloneID	OBa91983
Clone ID Related Accession (Same CDS sequence)	XM_015473632.1 , XM_015473632.2
Accession Version	XM_015473632.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329118.1
CDS	537..4235
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473632.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473632.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473632.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329118.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa91983
Clone ID Related Accession (Same CDS sequence)	XM_015473632.1 , XM_015473632.2
Accession Version	XM_015473632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329118.1
CDS	121..3819
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473632.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473632.2

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCTA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCAT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTACCTG TGCCCTCGTA CAAGTCCGTG 
TTTAGGTCCT ACTCGCAGGA TTTCGTGCCT CACAGCCAAG CTTCCGTCCA GCCTTTCCTT 
CCGTCTACCT CCTCTTCCTC TCCACACTTC CCACCTGTCC ACCAGTATCA GAGCTCTGAC 
TTAGCCGTGC CGCCCGTGGC CAGTCTGCCT CCCAGCACCC TGGACGGGCC TCTCTCATCT 
TCTCAGGAGA GCAGCTTTCA CGGGAACACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGCCCC AGACGCCTTC GAGTGAGCAC GCCTGTGAGG GGGACTACGT CAGTCTGTAT 
TGCTCTGGCC AGAGCAGCGA GGAGCTGGCC CGCCCTCGAG GAGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473633.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329119.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa91984
Clone ID Related Accession (Same CDS sequence)	XM_015473633.1
Accession Version	XM_015473633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAC 
GAGAAGTTCT CACCTTTCGC CGACACATTC AAGAAGCGTG TGAGCAAGAA CACGTTCTTC 
GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC CTGGTAGAGC TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTGGTG CGCAGCGGGG AGCTCAGCCT AGCACGCGTG 
CTCCGCAAGA ACATTCTGGA CAAGGTCGAC CAGAAGAAGC TGCTCCGGTG CGCCAACTCT 
GACCAGCCCC TGGCGGCCCG GGGAGTGGCA GCCAGGCCCG GGACCTTGCA CGACTTCCAC 
AGCCATGAAA TAGCCGAGCA GCTGACTCTG CTAGATGCCG AGCTCTTTTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG CAGAACGAGG AGAAGAGCCC GAACCTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCGATAAT CATGTTGCAG 
GAAAAGGCCC AAGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTA 
CGGAAGCTGA ACAACTTCAA CTCCTACTTG GCCATCCTCT CGGCGCTGGA CTCGGCCCCC 
ATTCGCAGGC TGGAGTGGCA GAAACAGACC TCAGAGGGCC TGGCCGAGTA CTGCACGCTG 
ATCGACAGCT CGTCATCCTT CCGCGCCTAC CGGGCCGCCC TCTCGGAGGT AGAACCGCCC 
TGCATCCCAT ACCTGGGGCT CATCCTGCAG GACCTCACCT TCGTCCACCT GGGGAACCCA 
GACTATATTG ACGGGAAGGT GAACTTCTCC AAACGCTGGC AGCAGTTCAA CATCCTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAC GACATCCGGA GAAATGACGA CATCATTAAC 
TTCTTCAACG ACTTCAGTGA CCACCTGGCC GAGGAGGCCC TATGGGAACT CTCTCTGAAA 
ATCAAACCCA GGAACATAAC GAGGAGGAAG ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329119.1
CDS	527..4180
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473633.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473633.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGTACAAGT CCGTGTTTAG GTCCTACTCG CAGGATTTCG TGCCTCACAG CCAAGCTTCC 
GTCCAGCCTT TCCTTCCGTC TACCTCCTCT TCCTCTCCAC ACTTCCCACC TGTCCACCAG 
TATCAGAGCT CTGACTTAGC CGTGCCGCCC GTGGCCAGTC TGCCTCCCAG CACCCTGGAC 
GGGCCTCTCT CATCTTCTCA GGAGAGCAGC TTTCACGGGA ACACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GCCCCAGACG CCTTCGAGTG AGCACGCCTG TGAGGGGGAC 
TACGTCAGTC TGTATTGCTC TGGCCAGAGC AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG 
TCACCCGCGG CGAAGGATGG ACACTCCAGA GACGCCACAT CGGCCAGCTG TGTGCCCGGG 
AAGGAGGGCA GGGACAGCGG GGACAGGGCC CGGAAGTCAC CAGACGCTCC AGAGGCTCAG 
TCAGAGGAGG AGGTGGACGA GCTCTCCCTC ATCGACCACA ACGAGATCAT GGCGAGGCTG 
ACCCTCAAGC AAGAGGGCGA TGACGGGCCA GACGTCCGTG GAGGGTCGGG CGACATCCTC 
CTCGTCCATG CCACGGAGAC GGACCGGAAA GATCTGGTGT TGTACTGCGA GGCCTTCCTG 
ACCACCTACA GGACCTTCAT CACTCCTGAG GAGCTCATCC AGAAGCTGCA GTACAGATAC 
GAGAAGTTCT CACCTTTCGC CGACACATTC AAGAAGCGTG TGAGCAAGAA CACGTTCTTC 
GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC CTGGTAGAGC TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTGGTG CGCAGCGGGG AGCTCAGCCT AGCACGCGTG 
CTCCGCAAGA ACATTCTGGA CAAGGTCGAC CAGAAGAAGC TGCTCCGGTG CGCCAACTCT 
GACCAGCCCC TGGCGGCCCG GGGAGTGGCA GCCAGGCCCG GGACCTTGCA CGACTTCCAC 
AGCCATGAAA TAGCCGAGCA GCTGACTCTG CTAGATGCCG AGCTCTTTTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG CAGAACGAGG AGAAGAGCCC GAACCTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCGATAAT CATGTTGCAG 
GAAAAGGCCC AAGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTA 
CGGAAGCTGA ACAACTTCAA CTCCTACTTG GCCATCCTCT CGGCGCTGGA CTCGGCCCCC 
ATTCGCAGGC TGGAGTGGCA GAAACAGACC TCAGAGGGCC TGGCCGAGTA CTGCACGCTG 
ATCGACAGCT CGTCATCCTT CCGCGCCTAC CGGGCCGCCC TCTCGGAGGT AGAACCGCCC 
TGCATCCCAT ACCTGGGGCT CATCCTGCAG GACCTCACCT TCGTCCACCT GGGGAACCCA 
GACTATATTG ACGGGAAGGT GAACTTCTCC AAACGCTGGC AGCAGTTCAA CATCCTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAC GACATCCGGA GAAATGACGA CATCATTAAC 
TTCTTCAACG ACTTCAGTGA CCACCTGGCC GAGGAGGCCC TATGGGAACT CTCTCTGAAA 
ATCAAACCCA GGAACATAAC GAGGAGGAAG ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473634.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329120.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

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CloneID	OBa91985
Clone ID Related Accession (Same CDS sequence)	XM_015473634.2 , XM_015473634.1
Accession Version	XM_015473634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGG CCTCCTGTGC TGCTTCTTCC TTCTCCTCTG TCTCCTACTG TGTCCAGCAA 
ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCATCAG TGTGTCCGTC 
TCTAACTCCT TTCTCAGCCG GCACGGCAGC TTACCTGTGC CCTCGTACAA GTCCGTGTTT 
AGGTCCTACT CGCAGGATTT CGTGCCTCAC AGCCAAGCTT CCGTCCAGCC TTTCCTTCCG 
TCTACCTCCT CTTCCTCTCC ACACTTCCCA CCTGTCCACC AGTATCAGAG CTCTGACTTA 
GCCGTGCCGC CCGTGGCCAG TCTGCCTCCC AGCACCCTGG ACGGGCCTCT CTCATCTTCT 
CAGGAGAGCA GCTTTCACGG GAACACTGTC TGCCTTCCTT CCGAAACCTC TTTCACTGAC 
TCGCCCCAGA CGCCTTCGGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329120.1
CDS	119..3733
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473634.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473634.2

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGG CCTCCTGTGC TGCTTCTTCC TTCTCCTCTG TCTCCTACTG TGTCCAGCAA 
ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCATCAG TGTGTCCGTC 
TCTAACTCCT TTCTCAGCCG GCACGGCAGC TTACCTGTGC CCTCGTACAA GTCCGTGTTT 
AGGTCCTACT CGCAGGATTT CGTGCCTCAC AGCCAAGCTT CCGTCCAGCC TTTCCTTCCG 
TCTACCTCCT CTTCCTCTCC ACACTTCCCA CCTGTCCACC AGTATCAGAG CTCTGACTTA 
GCCGTGCCGC CCGTGGCCAG TCTGCCTCCC AGCACCCTGG ACGGGCCTCT CTCATCTTCT 
CAGGAGAGCA GCTTTCACGG GAACACTGTC TGCCTTCCTT CCGAAACCTC TTTCACTGAC 
TCGCCCCAGA CGCCTTCGGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473634.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329120.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa91985
Clone ID Related Accession (Same CDS sequence)	XM_015473634.2 , XM_015473634.1
Accession Version	XM_015473634.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGG CCTCCTGTGC TGCTTCTTCC TTCTCCTCTG TCTCCTACTG TGTCCAGCAA 
ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCATCAG TGTGTCCGTC 
TCTAACTCCT TTCTCAGCCG GCACGGCAGC TTACCTGTGC CCTCGTACAA GTCCGTGTTT 
AGGTCCTACT CGCAGGATTT CGTGCCTCAC AGCCAAGCTT CCGTCCAGCC TTTCCTTCCG 
TCTACCTCCT CTTCCTCTCC ACACTTCCCA CCTGTCCACC AGTATCAGAG CTCTGACTTA 
GCCGTGCCGC CCGTGGCCAG TCTGCCTCCC AGCACCCTGG ACGGGCCTCT CTCATCTTCT 
CAGGAGAGCA GCTTTCACGG GAACACTGTC TGCCTTCCTT CCGAAACCTC TTTCACTGAC 
TCGCCCCAGA CGCCTTCGGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329120.1
CDS	530..4144
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473634.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473634.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCT 
ATTGATAAGA AGGTCCTGGA GATGCTCCCA GGGTCCGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAGCGA CCCTCGAATT CAGCACAGCT CAGCCCTCTC CTCCTGCTAC 
AGCCGAGTGT ACCAAAGTCT CGCCAATCTC ATCCGTTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGATAA AGAGATGGTG ACAACTGTGA AGGGGGTCAT CAAGGCCGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGACTC ACCATCGAGA AGCAGGGGCA TCCATCTCCA 
ACAAGCCCTG TGAAACCCAG CTCCCCAGCC TGCAAACCTG ACAGCCAGTC TGAGCTCCCC 
CTGACGGATC GAGAGATGGA GATACTAAAC AAGACGACCA GCCTGTCACA GTCAACCGAG 
GGGCTCCAAG ACTCTATGGA CGAAGAGGTT GCACCCCCCA AGCCCCCCTT ACCTGGCATT 
AGGGTGGTTG ATAACAGTCC TCCACCAGCA CTGCCCCCGA AGAAGAGACA GTCGGCTCCG 
TCCCCCACGC GTGTGGCCGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TTAATAGGCA GGACTTTGAC GTGGACTGTT ACGCACAGAG ACGGCTGTCG 
GGAGGCAGCC ACTCGTACAG CGGTGAGTCT CCCCGCCTGT CGCCCTGCAG CAGCATCGGC 
AAGCTCAGCA AGTCGGACGA GCAGCTGTCC TCTCTGGACA GGGACAGCGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGATCAT TACGATCCTG ACTACGAGTT CCTTCAGCAA 
GACCTCTCCA ACGCAGACCA GATACCTCAG CAAGTGGCCT GTAACCTCAG CCCGCTGCCA 
GAGTCCTTGG GGGAGTCTGG GTCTCCCTTT CTTGGCCATC CTCTCCAGTT GCCTCTGGGC 
AGCTGTCCCC AGCCAGATGG GTCCTCAGCC CCGGGGCAGC AGACGGACGT GCCGCCAGCC 
CTGCCCGAGA AGAAGCGCAG GAGTGCAGCC TCCCAGACCT TGGACAGCTC TGGCTGCCGG 
GCGTCCTACG AGCGACACCC CTCACAGTAC GACAACATCT CCGAGGATGA CCTGCAGAAC 
CCAGCCTCCG TCCAGTCCGT CCCCTTCACG CCCTTTGCTG CTATTCTTCC CTTTCAGCAA 
GGAGGTTCCT CAGCCTCTGT CGACTTCATG GGTGATTTCA CTGTTTCTGA ATCAACGGGT 
GACCCAGAAA AACCGCCACC TCTCCCCGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGCTGCTTG AGGACTACTC GGAGCCACAG CCTTCCATGT TCTACCAGAC GCCGCAGAAG 
GAACACGTGT ACCAGCAGAA GAACAAGCTT CTCATGGAGG TGTATGGCTT CAACGACTCC 
TTCAGCACCG GCGACGCCGC ACACGATCTC GCCCCGCCCC CCGCCCTGCC CCCCAAGCAG 
CGGCAGCTGG CCTCCTGTGC TGCTTCTTCC TTCTCCTCTG TCTCCTACTG TGTCCAGCAA 
ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCATCAG TGTGTCCGTC 
TCTAACTCCT TTCTCAGCCG GCACGGCAGC TTACCTGTGC CCTCGTACAA GTCCGTGTTT 
AGGTCCTACT CGCAGGATTT CGTGCCTCAC AGCCAAGCTT CCGTCCAGCC TTTCCTTCCG 
TCTACCTCCT CTTCCTCTCC ACACTTCCCA CCTGTCCACC AGTATCAGAG CTCTGACTTA 
GCCGTGCCGC CCGTGGCCAG TCTGCCTCCC AGCACCCTGG ACGGGCCTCT CTCATCTTCT 
CAGGAGAGCA GCTTTCACGG GAACACTGTC TGCCTTCCTT CCGAAACCTC TTTCACTGAC 
TCGCCCCAGA CGCCTTCGGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473635.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329121.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

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CloneID	OBa91986
Clone ID Related Accession (Same CDS sequence)	XM_015473635.1
Accession Version	XM_015473635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329121.1
CDS	517..3975
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473635.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473635.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCTACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCATCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTA CCTGTGCCCT CGGAGTCACC CGCGGCGAAG 
GATGGACACT CCAGAGACGC CACATCGGCC AGCTGTGTGC CCGGGAAGGA GGGCAGGGAC 
AGCGGGGACA GGGCCCGGAA GTCACCAGAC GCTCCAGAGG CTCAGTCAGA GGAGGAGGTG 
GACGAGCTCT CCCTCATCGA CCACAACGAG ATCATGGCGA GGCTGACCCT CAAGCAAGAG 
GGCGATGACG GGCCAGACGT CCGTGGAGGG TCGGGCGACA TCCTCCTCGT CCATGCCACG 
GAGACGGACC GGAAAGATCT GGTGTTGTAC TGCGAGGCCT TCCTGACCAC CTACAGGACC 
TTCATCACTC CTGAGGAGCT CATCCAGAAG CTGCAGTACA GATACGAGAA GTTCTCACCT 
TTCGCCGACA CATTCAAGAA GCGTGTGAGC AAGAACACGT TCTTCGTGCT GGTGCGGGTG 
GTGGACGAGC TCTGCCTGGT AGAGCTGACA GAAGAGATCC TGAAGCTGCT GATGGAGCTG 
GTCTTCCGCC TGGTGCGCAG CGGGGAGCTC AGCCTAGCAC GCGTGCTCCG CAAGAACATT 
CTGGACAAGG TCGACCAGAA GAAGCTGCTC CGGTGCGCCA ACTCTGACCA GCCCCTGGCG 
GCCCGGGGAG TGGCAGCCAG GCCCGGGACC TTGCACGACT TCCACAGCCA TGAAATAGCC 
GAGCAGCTGA CTCTGCTAGA TGCCGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCTA AAGAGCAGAA CGAGGAGAAG AGCCCGAACC TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCG ATAATCATGT TGCAGGAAAA GGCCCAAGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCGGCG CTGGACTCGG CCCCCATTCG CAGGCTGGAG 
TGGCAGAAAC AGACCTCAGA GGGCCTGGCC GAGTACTGCA CGCTGATCGA CAGCTCGTCA 
TCCTTCCGCG CCTACCGGGC CGCCCTCTCG GAGGTAGAAC CGCCCTGCAT CCCATACCTG 
GGGCTCATCC TGCAGGACCT CACCTTCGTC CACCTGGGGA ACCCAGACTA TATTGACGGG 
AAGGTGAACT TCTCCAAACG CTGGCAGCAG TTCAACATCC TGGACAGCAT GCGGTGCTTC 
CAGCAGGCGC ACTACGACAT CCGGAGAAAT GACGACATCA TTAACTTCTT CAACGACTTC 
AGTGACCACC TGGCCGAGGA GGCCCTATGG GAACTCTCTC TGAAAATCAA ACCCAGGAAC 
ATAACGAGGA GGAAGACAGA TAGAGAAGAA AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473636.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329122.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa91987
Clone ID Related Accession (Same CDS sequence)	XM_015473636.1
Accession Version	XM_015473636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGAGTGAGC ACGCCTGTGA GGGGGACTAC GTCAGTCTGT ATTGCTCTGG CCAGAGCAGC 
GAGGAGCTGG CCCGCCCTCG AGGAGAGTCA CCCGCGGCGA AGGATGGACA CTCCAGAGAC 
GCCACATCGG CCAGCTGTGT GCCCGGGAAG GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG 
AAGTCACCAG ACGCTCCAGA GGCTCAGTCA GAGGAGGAGG TGGACGAGCT CTCCCTCATC 
GACCACAACG AGATCATGGC GAGGCTGACC CTCAAGCAAG AGGGCGATGA CGGGCCAGAC 
GTCCGTGGAG GGTCGGGCGA CATCCTCCTC GTCCATGCCA CGGAGACGGA CCGGAAAGAT 
CTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCAC TCCTGAGGAG 
CTCATCCAGA AGCTGCAGTA CAGATACGAG AAGTTCTCAC CTTTCGCCGA CACATTCAAG 
AAGCGTGTGA GCAAGAACAC GTTCTTCGTG CTGGTGCGGG TGGTGGACGA GCTCTGCCTG 
GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTGGTGCGC 
AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC CGCAAGAACA TTCTGGACAA GGTCGACCAG 
AAGAAGCTGC TCCGGTGCGC CAACTCTGAC CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC 
AGGCCCGGGA CCTTGCACGA CTTCCACAGC CATGAAATAG CCGAGCAGCT GACTCTGCTA 
GATGCCGAGC TCTTTTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG 
AACGAGGAGA AGAGCCCGAA CCTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CGATAATCAT GTTGCAGGAA AAGGCCCAAG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTACGG AAGCTGAACA ACTTCAACTC CTACTTGGCC 
ATCCTCTCGG CGCTGGACTC GGCCCCCATT CGCAGGCTGG AGTGGCAGAA ACAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATC GACAGCTCGT CATCCTTCCG CGCCTACCGG 
GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC ATCCCATACC TGGGGCTCAT CCTGCAGGAC 
CTCACCTTCG TCCACCTGGG GAACCCAGAC TATATTGACG GGAAGGTGAA CTTCTCCAAA 
CGCTGGCAGC AGTTCAACAT CCTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTACGAC 
ATCCGGAGAA ATGACGACAT CATTAACTTC TTCAACGACT TCAGTGACCA CCTGGCCGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATC AAACCCAGGA ACATAACGAG GAGGAAGACA 
GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329122.1
CDS	333..3713
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473636.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473636.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGAGTGAGC ACGCCTGTGA GGGGGACTAC GTCAGTCTGT ATTGCTCTGG CCAGAGCAGC 
GAGGAGCTGG CCCGCCCTCG AGGAGAGTCA CCCGCGGCGA AGGATGGACA CTCCAGAGAC 
GCCACATCGG CCAGCTGTGT GCCCGGGAAG GAGGGCAGGG ACAGCGGGGA CAGGGCCCGG 
AAGTCACCAG ACGCTCCAGA GGCTCAGTCA GAGGAGGAGG TGGACGAGCT CTCCCTCATC 
GACCACAACG AGATCATGGC GAGGCTGACC CTCAAGCAAG AGGGCGATGA CGGGCCAGAC 
GTCCGTGGAG GGTCGGGCGA CATCCTCCTC GTCCATGCCA CGGAGACGGA CCGGAAAGAT 
CTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCAC TCCTGAGGAG 
CTCATCCAGA AGCTGCAGTA CAGATACGAG AAGTTCTCAC CTTTCGCCGA CACATTCAAG 
AAGCGTGTGA GCAAGAACAC GTTCTTCGTG CTGGTGCGGG TGGTGGACGA GCTCTGCCTG 
GTAGAGCTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTGGTGCGC 
AGCGGGGAGC TCAGCCTAGC ACGCGTGCTC CGCAAGAACA TTCTGGACAA GGTCGACCAG 
AAGAAGCTGC TCCGGTGCGC CAACTCTGAC CAGCCCCTGG CGGCCCGGGG AGTGGCAGCC 
AGGCCCGGGA CCTTGCACGA CTTCCACAGC CATGAAATAG CCGAGCAGCT GACTCTGCTA 
GATGCCGAGC TCTTTTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC TAAAGAGCAG 
AACGAGGAGA AGAGCCCGAA CCTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CGATAATCAT GTTGCAGGAA AAGGCCCAAG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTACGG AAGCTGAACA ACTTCAACTC CTACTTGGCC 
ATCCTCTCGG CGCTGGACTC GGCCCCCATT CGCAGGCTGG AGTGGCAGAA ACAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATC GACAGCTCGT CATCCTTCCG CGCCTACCGG 
GCCGCCCTCT CGGAGGTAGA ACCGCCCTGC ATCCCATACC TGGGGCTCAT CCTGCAGGAC 
CTCACCTTCG TCCACCTGGG GAACCCAGAC TATATTGACG GGAAGGTGAA CTTCTCCAAA 
CGCTGGCAGC AGTTCAACAT CCTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTACGAC 
ATCCGGAGAA ATGACGACAT CATTAACTTC TTCAACGACT TCAGTGACCA CCTGGCCGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATC AAACCCAGGA ACATAACGAG GAGGAAGACA 
GATAGAGAAG AAAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473637.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329123.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa91988
Clone ID Related Accession (Same CDS sequence)	XM_015473637.1
Accession Version	XM_015473637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTAG TCATTCTGAG ACAGTGGGCT GGTCATTTCC CCCTTCCAAG AGGAAGTGTC 
TTTATGCATG CGAAACTTCT CTGCGCGCTG TGGAGGTGTT TTGGGCGGGT AGGACATGTT 
TTGGGAAGTC ATGAAGATTA CCTGAGTCAA ACAATACTCG CCCATAGGGA GCTGGTCAGA 
CTCACCATCG AGAAGCAGGG GCATCCATCT CCAACAAGCC CTGTGAAACC CAGCTCCCCA 
GCCTGCAAAC CTGACAGCCA GTCTGAGCTC CCCCTGACGG ATCGAGAGAT GGAGATACTA 
AACAAGACGA CCAGCCTGTC ACAGTCAACC GAGGGGCTCC AAGACTCTAT GGACGAAGAG 
GTTGCACCCC CCAAGCCCCC CTTACCTGGC ATTAGGGTGG TTGATAACAG TCCTCCACCA 
GCACTGCCCC CGAAGAAGAG ACAGTCGGCT CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC 
CCCATGAGTC GAGCCACCAG TGGCTCCAGT TTGCCTGTTG GAATTAATAG GCAGGACTTT 
GACGTGGACT GTTACGCACA GAGACGGCTG TCGGGAGGCA GCCACTCGTA CAGCGGTGAG 
TCTCCCCGCC TGTCGCCCTG CAGCAGCATC GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG 
TCCTCTCTGG ACAGGGACAG CGGGCAGTGC TCCCGGAACA CAAGCTGTGA AACACTAGAT 
CATTACGATC CTGACTACGA GTTCCTTCAG CAAGACCTCT CCAACGCAGA CCAGATACCT 
CAGCAAGTGG CCTGTAACCT CAGCCCGCTG CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC 
TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA 
GCCCCGGGGC AGCAGACGGA CGTGCCGCCA GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA 
GCCTCCCAGA CCTTGGACAG CTCTGGCTGC CGGGCGTCCT ACGAGCGACA CCCCTCACAG 
TACGACAACA TCTCCGAGGA TGACCTGCAG AACCCAGCCT CCGTCCAGTC CGTCCCCTTC 
ACGCCCTTTG CTGCTATTCT TCCCTTTCAG CAAGGAGGTT CCTCAGCCTC TGTCGACTTC 
ATGGGTGATT TCACTGTTTC TGAATCAACG GGTGACCCAG AAAAACCGCC ACCTCTCCCC 
GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAGCTGC TTGAGGACTA CTCGGAGCCA 
CAGCCTTCCA TGTTCTACCA GACGCCGCAG AAGGAACACG TGTACCAGCA GAAGAACAAG 
CTTCTCATGG AGGTGTATGG CTTCAACGAC TCCTTCAGCA CCGGCGACGC CGCACACGAT 
CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT 
TCTTCCTTCT CCTCTGTCTC CTACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG 
GACGGCAGTG CCGCTCAGGG CATCAGTGTG TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC 
GGCAGCTTAC CTGTGCCCTC GTACAAGTCC GTGTTTAGGT CCTACTCGCA GGATTTCGTG 
CCTCACAGCC AAGCTTCCGT CCAGCCTTTC CTTCCGTCTA CCTCCTCTTC CTCTCCACAC 
TTCCCACCTG TCCACCAGTA TCAGAGCTCT GACTTAGCCG TGCCGCCCGT GGCCAGTCTG 
CCTCCCAGCA CCCTGGACGG GCCTCTCTCA TCTTCTCAGG AGAGCAGCTT TCACGGGAAC 
ACTGTCTGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGC CCCAGACGCC TTCGAGTGAG 
CACGCCTGTG AGGGGGACTA CGTCAGTCTG TATTGCTCTG GCCAGAGCAG CGAGGAGCTG 
GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329123.1
CDS	441..3752
Translation	MNLVILRQWAGHFPLPRGSVFMHAKLLCALWRCFGRVGHVLGSHEDYLSQTILAHRELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473637.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473637.1

ORF Insert Sequence:

ATGAATTTAG TCATTCTGAG ACAGTGGGCT GGTCATTTCC CCCTTCCAAG AGGAAGTGTC 
TTTATGCATG CGAAACTTCT CTGCGCGCTG TGGAGGTGTT TTGGGCGGGT AGGACATGTT 
TTGGGAAGTC ATGAAGATTA CCTGAGTCAA ACAATACTCG CCCATAGGGA GCTGGTCAGA 
CTCACCATCG AGAAGCAGGG GCATCCATCT CCAACAAGCC CTGTGAAACC CAGCTCCCCA 
GCCTGCAAAC CTGACAGCCA GTCTGAGCTC CCCCTGACGG ATCGAGAGAT GGAGATACTA 
AACAAGACGA CCAGCCTGTC ACAGTCAACC GAGGGGCTCC AAGACTCTAT GGACGAAGAG 
GTTGCACCCC CCAAGCCCCC CTTACCTGGC ATTAGGGTGG TTGATAACAG TCCTCCACCA 
GCACTGCCCC CGAAGAAGAG ACAGTCGGCT CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC 
CCCATGAGTC GAGCCACCAG TGGCTCCAGT TTGCCTGTTG GAATTAATAG GCAGGACTTT 
GACGTGGACT GTTACGCACA GAGACGGCTG TCGGGAGGCA GCCACTCGTA CAGCGGTGAG 
TCTCCCCGCC TGTCGCCCTG CAGCAGCATC GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG 
TCCTCTCTGG ACAGGGACAG CGGGCAGTGC TCCCGGAACA CAAGCTGTGA AACACTAGAT 
CATTACGATC CTGACTACGA GTTCCTTCAG CAAGACCTCT CCAACGCAGA CCAGATACCT 
CAGCAAGTGG CCTGTAACCT CAGCCCGCTG CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC 
TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA 
GCCCCGGGGC AGCAGACGGA CGTGCCGCCA GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA 
GCCTCCCAGA CCTTGGACAG CTCTGGCTGC CGGGCGTCCT ACGAGCGACA CCCCTCACAG 
TACGACAACA TCTCCGAGGA TGACCTGCAG AACCCAGCCT CCGTCCAGTC CGTCCCCTTC 
ACGCCCTTTG CTGCTATTCT TCCCTTTCAG CAAGGAGGTT CCTCAGCCTC TGTCGACTTC 
ATGGGTGATT TCACTGTTTC TGAATCAACG GGTGACCCAG AAAAACCGCC ACCTCTCCCC 
GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAGCTGC TTGAGGACTA CTCGGAGCCA 
CAGCCTTCCA TGTTCTACCA GACGCCGCAG AAGGAACACG TGTACCAGCA GAAGAACAAG 
CTTCTCATGG AGGTGTATGG CTTCAACGAC TCCTTCAGCA CCGGCGACGC CGCACACGAT 
CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT 
TCTTCCTTCT CCTCTGTCTC CTACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG 
GACGGCAGTG CCGCTCAGGG CATCAGTGTG TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC 
GGCAGCTTAC CTGTGCCCTC GTACAAGTCC GTGTTTAGGT CCTACTCGCA GGATTTCGTG 
CCTCACAGCC AAGCTTCCGT CCAGCCTTTC CTTCCGTCTA CCTCCTCTTC CTCTCCACAC 
TTCCCACCTG TCCACCAGTA TCAGAGCTCT GACTTAGCCG TGCCGCCCGT GGCCAGTCTG 
CCTCCCAGCA CCCTGGACGG GCCTCTCTCA TCTTCTCAGG AGAGCAGCTT TCACGGGAAC 
ACTGTCTGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGC CCCAGACGCC TTCGAGTGAG 
CACGCCTGTG AGGGGGACTA CGTCAGTCTG TATTGCTCTG GCCAGAGCAG CGAGGAGCTG 
GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG AAGGATGGAC ACTCCAGAGA CGCCACATCG 
GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG GACAGCGGGG ACAGGGCCCG GAAGTCACCA 
GACGCTCCAG AGGCTCAGTC AGAGGAGGAG GTGGACGAGC TCTCCCTCAT CGACCACAAC 
GAGATCATGG CGAGGCTGAC CCTCAAGCAA GAGGGCGATG ACGGGCCAGA CGTCCGTGGA 
GGGTCGGGCG ACATCCTCCT CGTCCATGCC ACGGAGACGG ACCGGAAAGA TCTGGTGTTG 
TACTGCGAGG CCTTCCTGAC CACCTACAGG ACCTTCATCA CTCCTGAGGA GCTCATCCAG 
AAGCTGCAGT ACAGATACGA GAAGTTCTCA CCTTTCGCCG ACACATTCAA GAAGCGTGTG 
AGCAAGAACA CGTTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTAGAGCTG 
ACAGAAGAGA TCCTGAAGCT GCTGATGGAG CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG 
CTCAGCCTAG CACGCGTGCT CCGCAAGAAC ATTCTGGACA AGGTCGACCA GAAGAAGCTG 
CTCCGGTGCG CCAACTCTGA CCAGCCCCTG GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG 
ACCTTGCACG ACTTCCACAG CCATGAAATA GCCGAGCAGC TGACTCTGCT AGATGCCGAG 
CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CTAAAGAGCA GAACGAGGAG 
AAGAGCCCGA ACCTGACCCA GTTCACGGAG CACTTCAACA ACATGTCCTA CTGGGTCCGG 
TCGATAATCA TGTTGCAGGA AAAGGCCCAA GACAGGGAAC GGCTGCTCTT GAAGTTCATC 
AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTCAACT CCTACTTGGC CATCCTCTCG 
GCGCTGGACT CGGCCCCCAT TCGCAGGCTG GAGTGGCAGA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACGCTGAT CGACAGCTCG TCATCCTTCC GCGCCTACCG GGCCGCCCTC 
TCGGAGGTAG AACCGCCCTG CATCCCATAC CTGGGGCTCA TCCTGCAGGA CCTCACCTTC 
GTCCACCTGG GGAACCCAGA CTATATTGAC GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG 
CAGTTCAACA TCCTGGACAG CATGCGGTGC TTCCAGCAGG CGCACTACGA CATCCGGAGA 
AATGACGACA TCATTAACTT CTTCAACGAC TTCAGTGACC ACCTGGCCGA GGAGGCCCTA 
TGGGAACTCT CTCTGAAAAT CAAACCCAGG AACATAACGA GGAGGAAGAC AGATAGAGAA 
GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473638.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329124.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa91989
Clone ID Related Accession (Same CDS sequence)	XM_015473638.1 , XM_015473638.2
Accession Version	XM_015473638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329124.1
CDS	306..3605
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473638.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473638.1

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473638.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329124.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OBa91989
Clone ID Related Accession (Same CDS sequence)	XM_015473638.1 , XM_015473638.2
Accession Version	XM_015473638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329124.1
CDS	108..3407
Translation	MCPAHGRAPSCITAASNLTYATSDISDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473638.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473638.2

ORF Insert Sequence:

ATGTGCCCCG CACATGGACG AGCACCTTCC TGCATTACCG CAGCCAGTAA TTTAACCTAC 
GCCACATCAG ACATCTCAGA CTCTCAGCGT TCTCATCTCT CCTCCTTCAC CATGAAGCTG 
AAGGACAAAT TCCACTCGCC CAAAATCAAG AGGACGCCAT CAAAGAAGGG AAAACCAGCC 
GAGGTGTCTG TGAAGATTCC AGAGAAGCCC GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTATCCC CTTGGATCTG GAGCAGCAGG CAGTAGAGTT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCTT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCTATTGAT 
AAGAAGGTCC TGGAGATGCT CCCAGGGTCC GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA GCGACCCTCG AATTCAGCAC AGCTCAGCCC TCTCCTCCTG CTACAGCCGA 
GTGTACCAAA GTCTCGCCAA TCTCATCCGT TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ATAAAGAGAT GGTGACAACT GTGAAGGGGG TCATCAAGGC CGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG ACTCACCATC GAGAAGCAGG GGCATCCATC TCCAACAAGC 
CCTGTGAAAC CCAGCTCCCC AGCCTGCAAA CCTGACAGCC AGTCTGAGCT CCCCCTGACG 
GATCGAGAGA TGGAGATACT AAACAAGACG ACCAGCCTGT CACAGTCAAC CGAGGGGCTC 
CAAGACTCTA TGGACGAAGA GGTTGCACCC CCCAAGCCCC CCTTACCTGG CATTAGGGTG 
GTTGATAACA GTCCTCCACC AGCACTGCCC CCGAAGAAGA GACAGTCGGC TCCGTCCCCC 
ACGCGTGTGG CCGTTGTGGC CCCCATGAGT CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATTAATA GGCAGGACTT TGACGTGGAC TGTTACGCAC AGAGACGGCT GTCGGGAGGC 
AGCCACTCGT ACAGCGGTGA GTCTCCCCGC CTGTCGCCCT GCAGCAGCAT CGGCAAGCTC 
AGCAAGTCGG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GCGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA TCATTACGAT CCTGACTACG AGTTCCTTCA GCAAGACCTC 
TCCAACGCAG ACCAGATACC TCAGCAAGTG GCCTGTAACC TCAGCCCGCT GCCAGAGTCC 
TTGGGGGAGT CTGGGTCTCC CTTTCTTGGC CATCCTCTCC AGTTGCCTCT GGGCAGCTGT 
CCCCAGCCAG ATGGGTCCTC AGCCCCGGGG CAGCAGACGG ACGTGCCGCC AGCCCTGCCC 
GAGAAGAAGC GCAGGAGTGC AGCCTCCCAG ACCTTGGACA GCTCTGGCTG CCGGGCGTCC 
TACGAGCGAC ACCCCTCACA GTACGACAAC ATCTCCGAGG ATGACCTGCA GAACCCAGCC 
TCCGTCCAGT CCGTCCCCTT CACGCCCTTT GCTGCTATTC TTCCCTTTCA GCAAGGAGGT 
TCCTCAGCCT CTGTCGACTT CATGGGTGAT TTCACTGTTT CTGAATCAAC GGGTGACCCA 
GAAAAACCGC CACCTCTCCC CGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG 
CTTGAGGACT ACTCGGAGCC ACAGCCTTCC ATGTTCTACC AGACGCCGCA GAAGGAACAC 
GTGTACCAGC AGAAGAACAA GCTTCTCATG GAGGTGTATG GCTTCAACGA CTCCTTCAGC 
ACCGGCGACG CCGCACACGA TCTCGCCCCG CCCCCCGCCC TGCCCCCCAA GCAGCGGCAG 
CTGGAGTCAC CCGCGGCGAA GGATGGACAC TCCAGAGACG CCACATCGGC CAGCTGTGTG 
CCCGGGAAGG AGGGCAGGGA CAGCGGGGAC AGGGCCCGGA AGTCACCAGA CGCTCCAGAG 
GCTCAGTCAG AGGAGGAGGT GGACGAGCTC TCCCTCATCG ACCACAACGA GATCATGGCG 
AGGCTGACCC TCAAGCAAGA GGGCGATGAC GGGCCAGACG TCCGTGGAGG GTCGGGCGAC 
ATCCTCCTCG TCCATGCCAC GGAGACGGAC CGGAAAGATC TGGTGTTGTA CTGCGAGGCC 
TTCCTGACCA CCTACAGGAC CTTCATCACT CCTGAGGAGC TCATCCAGAA GCTGCAGTAC 
AGATACGAGA AGTTCTCACC TTTCGCCGAC ACATTCAAGA AGCGTGTGAG CAAGAACACG 
TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TAGAGCTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTGGTGCGCA GCGGGGAGCT CAGCCTAGCA 
CGCGTGCTCC GCAAGAACAT TCTGGACAAG GTCGACCAGA AGAAGCTGCT CCGGTGCGCC 
AACTCTGACC AGCCCCTGGC GGCCCGGGGA GTGGCAGCCA GGCCCGGGAC CTTGCACGAC 
TTCCACAGCC ATGAAATAGC CGAGCAGCTG ACTCTGCTAG ATGCCGAGCT CTTTTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCT AAAGAGCAGA ACGAGGAGAA GAGCCCGAAC 
CTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC GATAATCATG 
TTGCAGGAAA AGGCCCAAGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTACGGA AGCTGAACAA CTTCAACTCC TACTTGGCCA TCCTCTCGGC GCTGGACTCG 
GCCCCCATTC GCAGGCTGGA GTGGCAGAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACGCTGATCG ACAGCTCGTC ATCCTTCCGC GCCTACCGGG CCGCCCTCTC GGAGGTAGAA 
CCGCCCTGCA TCCCATACCT GGGGCTCATC CTGCAGGACC TCACCTTCGT CCACCTGGGG 
AACCCAGACT ATATTGACGG GAAGGTGAAC TTCTCCAAAC GCTGGCAGCA GTTCAACATC 
CTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTACGACA TCCGGAGAAA TGACGACATC 
ATTAACTTCT TCAACGACTT CAGTGACCAC CTGGCCGAGG AGGCCCTATG GGAACTCTCT 
CTGAAAATCA AACCCAGGAA CATAACGAGG AGGAAGACAG ATAGAGAAGA AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473639.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329125.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

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CloneID	OBa91990
Clone ID Related Accession (Same CDS sequence)	XM_015473639.1 , XM_015473639.2
Accession Version	XM_015473639.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329125.1
CDS	14..3295
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473639.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473639.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473639.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329125.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OBa91990
Clone ID Related Accession (Same CDS sequence)	XM_015473639.1 , XM_015473639.2
Accession Version	XM_015473639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329125.1
CDS	65..3346
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473639.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473639.2

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473640.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329126.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa91991
Clone ID Related Accession (Same CDS sequence)	XM_015473640.1
Accession Version	XM_015473640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC TCGTACAAGT CCGTGTTTAG GTCCTACTCG 
CAGGATTTCG TGCCTCACAG CCAAGCTTCC GTCCAGCCTT TCCTTCCGTC TACCTCCTCT 
TCCTCTCCAC ACTTCCCACC TGTCCACCAG TATCAGAGCT CTGACTTAGC CGTGCCGCCC 
GTGGCCAGTC TGCCTCCCAG CACCCTGGAC GGGCCTCTCT CATCTTCTCA GGAGAGCAGC 
TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG 
CCTTCGAGTG AGCACGCCTG TGAGGGGGAC TACGTCAGTC TGTATTGCTC TGGCCAGAGC 
AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG TCACCCGCGG CGAAGGATGG ACACTCCAGA 
GACGCCACAT CGGCCAGCTG TGTGCCCGGG AAGGAGGGCA GGGACAGCGG GGACAGGGCC 
CGGAAGTCAC CAGACGCTCC AGAGGCTCAG TCAGAGGAGG AGGTGGACGA GCTCTCCCTC 
ATCGACCACA ACGAGATCAT GGCGAGGCTG ACCCTCAAGC AAGAGGGCGA TGACGGGCCA 
GACGTCCGTG GAGGGTCGGG CGACATCCTC CTCGTCCATG CCACGGAGAC GGACCGGAAA 
GATCTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CACTCCTGAG 
GAGCTCATCC AGAAGCTGCA GTACAGATAC GAGAAGTTCT CACCTTTCGC CGACACATTC 
AAGAAGCGTG TGAGCAAGAA CACGTTCTTC GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC 
CTGGTAGAGC TGACAGAAGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTGGTG 
CGCAGCGGGG AGCTCAGCCT AGCACGCGTG CTCCGCAAGA ACATTCTGGA CAAGGTCGAC 
CAGAAGAAGC TGCTCCGGTG CGCCAACTCT GACCAGCCCC TGGCGGCCCG GGGAGTGGCA 
GCCAGGCCCG GGACCTTGCA CGACTTCCAC AGCCATGAAA TAGCCGAGCA GCTGACTCTG 
CTAGATGCCG AGCTCTTTTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG 
CAGAACGAGG AGAAGAGCCC GAACCTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCGATAAT CATGTTGCAG GAAAAGGCCC AAGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTA CGGAAGCTGA ACAACTTCAA CTCCTACTTG 
GCCATCCTCT CGGCGCTGGA CTCGGCCCCC ATTCGCAGGC TGGAGTGGCA GAAACAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATCGACAGCT CGTCATCCTT CCGCGCCTAC 
CGGGCCGCCC TCTCGGAGGT AGAACCGCCC TGCATCCCAT ACCTGGGGCT CATCCTGCAG 
GACCTCACCT TCGTCCACCT GGGGAACCCA GACTATATTG ACGGGAAGGT GAACTTCTCC 
AAACGCTGGC AGCAGTTCAA CATCCTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAC 
GACATCCGGA GAAATGACGA CATCATTAAC TTCTTCAACG ACTTCAGTGA CCACCTGGCC 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATCAAACCCA GGAACATAAC GAGGAGGAAG 
ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329126.1
CDS	432..3455
Translation	MEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473640.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473640.1

ORF Insert Sequence:

ATGGAGATAC TAAACAAGAC GACCAGCCTG TCACAGTCAA CCGAGGGGCT CCAAGACTCT 
ATGGACGAAG AGGTTGCACC CCCCAAGCCC CCCTTACCTG GCATTAGGGT GGTTGATAAC 
AGTCCTCCAC CAGCACTGCC CCCGAAGAAG AGACAGTCGG CTCCGTCCCC CACGCGTGTG 
GCCGTTGTGG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT 
AGGCAGGACT TTGACGTGGA CTGTTACGCA CAGAGACGGC TGTCGGGAGG CAGCCACTCG 
TACAGCGGTG AGTCTCCCCG CCTGTCGCCC TGCAGCAGCA TCGGCAAGCT CAGCAAGTCG 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ATCATTACGA TCCTGACTAC GAGTTCCTTC AGCAAGACCT CTCCAACGCA 
GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTCAGCCCGC TGCCAGAGTC CTTGGGGGAG 
TCTGGGTCTC CCTTTCTTGG CCATCCTCTC CAGTTGCCTC TGGGCAGCTG TCCCCAGCCA 
GATGGGTCCT CAGCCCCGGG GCAGCAGACG GACGTGCCGC CAGCCCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACCTTGGAC AGCTCTGGCT GCCGGGCGTC CTACGAGCGA 
CACCCCTCAC AGTACGACAA CATCTCCGAG GATGACCTGC AGAACCCAGC CTCCGTCCAG 
TCCGTCCCCT TCACGCCCTT TGCTGCTATT CTTCCCTTTC AGCAAGGAGG TTCCTCAGCC 
TCTGTCGACT TCATGGGTGA TTTCACTGTT TCTGAATCAA CGGGTGACCC AGAAAAACCG 
CCACCTCTCC CCGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTTGAGGAC 
TACTCGGAGC CACAGCCTTC CATGTTCTAC CAGACGCCGC AGAAGGAACA CGTGTACCAG 
CAGAAGAACA AGCTTCTCAT GGAGGTGTAT GGCTTCAACG ACTCCTTCAG CACCGGCGAC 
GCCGCACACG ATCTCGCCCC GCCCCCCGCC CTGCCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT ACCTGTGCCC TCGTACAAGT CCGTGTTTAG GTCCTACTCG 
CAGGATTTCG TGCCTCACAG CCAAGCTTCC GTCCAGCCTT TCCTTCCGTC TACCTCCTCT 
TCCTCTCCAC ACTTCCCACC TGTCCACCAG TATCAGAGCT CTGACTTAGC CGTGCCGCCC 
GTGGCCAGTC TGCCTCCCAG CACCCTGGAC GGGCCTCTCT CATCTTCTCA GGAGAGCAGC 
TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG 
CCTTCGAGTG AGCACGCCTG TGAGGGGGAC TACGTCAGTC TGTATTGCTC TGGCCAGAGC 
AGCGAGGAGC TGGCCCGCCC TCGAGGAGAG TCACCCGCGG CGAAGGATGG ACACTCCAGA 
GACGCCACAT CGGCCAGCTG TGTGCCCGGG AAGGAGGGCA GGGACAGCGG GGACAGGGCC 
CGGAAGTCAC CAGACGCTCC AGAGGCTCAG TCAGAGGAGG AGGTGGACGA GCTCTCCCTC 
ATCGACCACA ACGAGATCAT GGCGAGGCTG ACCCTCAAGC AAGAGGGCGA TGACGGGCCA 
GACGTCCGTG GAGGGTCGGG CGACATCCTC CTCGTCCATG CCACGGAGAC GGACCGGAAA 
GATCTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CACTCCTGAG 
GAGCTCATCC AGAAGCTGCA GTACAGATAC GAGAAGTTCT CACCTTTCGC CGACACATTC 
AAGAAGCGTG TGAGCAAGAA CACGTTCTTC GTGCTGGTGC GGGTGGTGGA CGAGCTCTGC 
CTGGTAGAGC TGACAGAAGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTGGTG 
CGCAGCGGGG AGCTCAGCCT AGCACGCGTG CTCCGCAAGA ACATTCTGGA CAAGGTCGAC 
CAGAAGAAGC TGCTCCGGTG CGCCAACTCT GACCAGCCCC TGGCGGCCCG GGGAGTGGCA 
GCCAGGCCCG GGACCTTGCA CGACTTCCAC AGCCATGAAA TAGCCGAGCA GCTGACTCTG 
CTAGATGCCG AGCTCTTTTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCTAAAGAG 
CAGAACGAGG AGAAGAGCCC GAACCTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCGATAAT CATGTTGCAG GAAAAGGCCC AAGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTA CGGAAGCTGA ACAACTTCAA CTCCTACTTG 
GCCATCCTCT CGGCGCTGGA CTCGGCCCCC ATTCGCAGGC TGGAGTGGCA GAAACAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATCGACAGCT CGTCATCCTT CCGCGCCTAC 
CGGGCCGCCC TCTCGGAGGT AGAACCGCCC TGCATCCCAT ACCTGGGGCT CATCCTGCAG 
GACCTCACCT TCGTCCACCT GGGGAACCCA GACTATATTG ACGGGAAGGT GAACTTCTCC 
AAACGCTGGC AGCAGTTCAA CATCCTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAC 
GACATCCGGA GAAATGACGA CATCATTAAC TTCTTCAACG ACTTCAGTGA CCACCTGGCC 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATCAAACCCA GGAACATAAC GAGGAGGAAG 
ACAGATAGAG AAGAAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( NM_001205872.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192801.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), mRNA.

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CloneID	OBa40953
Clone ID Related Accession (Same CDS sequence)	NM_001205872.1
Accession Version	NM_001205872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On Apr 20, 2011 this sequence version replaced XM_598698.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGGA GTCACCCGCG 
GCGAAGGATG GACACTCCAG AGACGCCACA TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC 
AGGGACAGCG GGGACAGGGC CCGGAAGTCA CCAGACGCTC CAGAGGCTCA GTCAGAGGAG 
GAGGTGGACG AGCTCTCCCT CATCGACCAC AACGAGATCA TGGCGAGGCT GACCCTCAAG 
CAAGAGGGCG ATGACGGGCC AGACGTCCGT GGAGGGTCGG GCGACATCCT CCTCGTCCAT 
GCCACGGAGA CGGACCGGAA AGATCTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCACTCCTGA GGAGCTCATC CAGAAGCTGC AGTACAGATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192801.1
CDS	1..3285
Translation	MGNAVEKQKPLKRSHLCPWKQDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_001205872.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205872.1

ORF Insert Sequence:

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGGA GTCACCCGCG 
GCGAAGGATG GACACTCCAG AGACGCCACA TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC 
AGGGACAGCG GGGACAGGGC CCGGAAGTCA CCAGACGCTC CAGAGGCTCA GTCAGAGGAG 
GAGGTGGACG AGCTCTCCCT CATCGACCAC AACGAGATCA TGGCGAGGCT GACCCTCAAG 
CAAGAGGGCG ATGACGGGCC AGACGTCCGT GGAGGGTCGG GCGACATCCT CCTCGTCCAT 
GCCACGGAGA CGGACCGGAA AGATCTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCACTCCTGA GGAGCTCATC CAGAAGCTGC AGTACAGATA CGAGAAGTTC 
TCACCTTTCG CCGACACATT CAAGAAGCGT GTGAGCAAGA ACACGTTCTT CGTGCTGGTG 
CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG CTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG GAGCTCAGCC TAGCACGCGT GCTCCGCAAG 
AACATTCTGG ACAAGGTCGA CCAGAAGAAG CTGCTCCGGT GCGCCAACTC TGACCAGCCC 
CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC GGGACCTTGC ACGACTTCCA CAGCCATGAA 
ATAGCCGAGC AGCTGACTCT GCTAGATGCC GAGCTCTTTT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCTAAAGA GCAGAACGAG GAGAAGAGCC CGAACCTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCGATAA TCATGTTGCA GGAAAAGGCC 
CAAGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT ACGGAAGCTG 
AACAACTTCA ACTCCTACTT GGCCATCCTC TCGGCGCTGG ACTCGGCCCC CATTCGCAGG 
CTGGAGTGGC AGAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATCGACAGC 
TCGTCATCCT TCCGCGCCTA CCGGGCCGCC CTCTCGGAGG TAGAACCGCC CTGCATCCCA 
TACCTGGGGC TCATCCTGCA GGACCTCACC TTCGTCCACC TGGGGAACCC AGACTATATT 
GACGGGAAGG TGAACTTCTC CAAACGCTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA CGACATCCGG AGAAATGACG ACATCATTAA CTTCTTCAAC 
GACTTCAGTG ACCACCTGGC CGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATCAAACCC 
AGGAACATAA CGAGGAGGAA GACAGATAGA GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_005213261.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213318.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OBa24398
Clone ID Related Accession (Same CDS sequence)	XM_005213261.3
Accession Version	XM_005213261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCATC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCACGGCA GCTTACCTGT GCCCTCGTAC AAGTCCGTGT TTAGGTCCTA CTCGCAGGAT 
TTCGTGCCTC ACAGCCAAGC TTCCGTCCAG CCTTTCCTTC CGTCTACCTC CTCTTCCTCT 
CCACACTTCC CACCTGTCCA CCAGTATCAG AGCTCTGACT TAGCCGTGCC GCCCGTGGCC 
AGTCTGCCTC CCAGCACCCT GGACGGGCCT CTCTCATCTT CTCAGGAGAG CAGCTTTCAC 
GGGAACACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGCCCCA GACGCCTTCG 
AGTGAGCACG CCTGTGAGGG GGACTACGTC AGTCTGTATT GCTCTGGCCA GAGCAGCGAG 
GAGCTGGCCC GCCCTCGAGG AGAGTCACCC GCGGCGAAGG ATGGACACTC CAGAGACGCC 
ACATCGGCCA GCTGTGTGCC CGGGAAGGAG GGCAGGGACA GCGGGGACAG GGCCCGGAAG 
TCACCAGACG CTCCAGAGGC TCAGTCAGAG GAGGAGGTGG ACGAGCTCTC CCTCATCGAC 
CACAACGAGA TCATGGCGAG GCTGACCCTC AAGCAAGAGG GCGATGACGG GCCAGACGTC 
CGTGGAGGGT CGGGCGACAT CCTCCTCGTC CATGCCACGG AGACGGACCG GAAAGATCTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCACTCC TGAGGAGCTC 
ATCCAGAAGC TGCAGTACAG ATACGAGAAG TTCTCACCTT TCGCCGACAC ATTCAAGAAG 
CGTGTGAGCA AGAACACGTT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTA 
GAGCTGACAG AAGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT GGTGCGCAGC 
GGGGAGCTCA GCCTAGCACG CGTGCTCCGC AAGAACATTC TGGACAAGGT CGACCAGAAG 
AAGCTGCTCC GGTGCGCCAA CTCTGACCAG CCCCTGGCGG CCCGGGGAGT GGCAGCCAGG 
CCCGGGACCT TGCACGACTT CCACAGCCAT GAAATAGCCG AGCAGCTGAC TCTGCTAGAT 
GCCGAGCTCT TTTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCTAA AGAGCAGAAC 
GAGGAGAAGA GCCCGAACCT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCGA TAATCATGTT GCAGGAAAAG GCCCAAGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTACGGAAG CTGAACAACT TCAACTCCTA CTTGGCCATC 
CTCTCGGCGC TGGACTCGGC CCCCATTCGC AGGCTGGAGT GGCAGAAACA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATCGAC AGCTCGTCAT CCTTCCGCGC CTACCGGGCC 
GCCCTCTCGG AGGTAGAACC GCCCTGCATC CCATACCTGG GGCTCATCCT GCAGGACCTC 
ACCTTCGTCC ACCTGGGGAA CCCAGACTAT ATTGACGGGA AGGTGAACTT CTCCAAACGC 
TGGCAGCAGT TCAACATCCT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTACGACATC 
CGGAGAAATG ACGACATCAT TAACTTCTTC AACGACTTCA GTGACCACCT GGCCGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATCAAA CCCAGGAACA TAACGAGGAG GAAGACAGAT 
AGAGAAGAAA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213318.1
CDS	1317..5114
Translation	MGNAVEKQKPLKRSHLCPWKQDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_005213261.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213261.3

ORF Insert Sequence:

ATGGGCAATG CTGTTGAAAA ACAGAAGCCC CTGAAACGAA GCCACCTGTG TCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC 
TCGCCCAAAA TCAAGAGGAC GCCATCAAAG AAGGGAAAAC CAGCCGAGGT GTCTGTGAAG 
ATTCCAGAGA AGCCCGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
ATCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAGTTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCTA TTGATAAGAA GGTCCTGGAG 
ATGCTCCCAG GGTCCGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAC 
CCTCGAATTC AGCACAGCTC AGCCCTCTCC TCCTGCTACA GCCGAGTGTA CCAAAGTCTC 
GCCAATCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGATAAA 
GAGATGGTGA CAACTGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGACTCA CCATCGAGAA GCAGGGGCAT CCATCTCCAA CAAGCCCTGT GAAACCCAGC 
TCCCCAGCCT GCAAACCTGA CAGCCAGTCT GAGCTCCCCC TGACGGATCG AGAGATGGAG 
ATACTAAACA AGACGACCAG CCTGTCACAG TCAACCGAGG GGCTCCAAGA CTCTATGGAC 
GAAGAGGTTG CACCCCCCAA GCCCCCCTTA CCTGGCATTA GGGTGGTTGA TAACAGTCCT 
CCACCAGCAC TGCCCCCGAA GAAGAGACAG TCGGCTCCGT CCCCCACGCG TGTGGCCGTT 
GTGGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG 
GACTTTGACG TGGACTGTTA CGCACAGAGA CGGCTGTCGG GAGGCAGCCA CTCGTACAGC 
GGTGAGTCTC CCCGCCTGTC GCCCTGCAGC AGCATCGGCA AGCTCAGCAA GTCGGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGCGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGATCATT ACGATCCTGA CTACGAGTTC CTTCAGCAAG ACCTCTCCAA CGCAGACCAG 
ATACCTCAGC AAGTGGCCTG TAACCTCAGC CCGCTGCCAG AGTCCTTGGG GGAGTCTGGG 
TCTCCCTTTC TTGGCCATCC TCTCCAGTTG CCTCTGGGCA GCTGTCCCCA GCCAGATGGG 
TCCTCAGCCC CGGGGCAGCA GACGGACGTG CCGCCAGCCC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACCTT GGACAGCTCT GGCTGCCGGG CGTCCTACGA GCGACACCCC 
TCACAGTACG ACAACATCTC CGAGGATGAC CTGCAGAACC CAGCCTCCGT CCAGTCCGTC 
CCCTTCACGC CCTTTGCTGC TATTCTTCCC TTTCAGCAAG GAGGTTCCTC AGCCTCTGTC 
GACTTCATGG GTGATTTCAC TGTTTCTGAA TCAACGGGTG ACCCAGAAAA ACCGCCACCT 
CTCCCCGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTTGA GGACTACTCG 
GAGCCACAGC CTTCCATGTT CTACCAGACG CCGCAGAAGG AACACGTGTA CCAGCAGAAG 
AACAAGCTTC TCATGGAGGT GTATGGCTTC AACGACTCCT TCAGCACCGG CGACGCCGCA 
CACGATCTCG CCCCGCCCCC CGCCCTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCATC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCACGGCA GCTTACCTGT GCCCTCGTAC AAGTCCGTGT TTAGGTCCTA CTCGCAGGAT 
TTCGTGCCTC ACAGCCAAGC TTCCGTCCAG CCTTTCCTTC CGTCTACCTC CTCTTCCTCT 
CCACACTTCC CACCTGTCCA CCAGTATCAG AGCTCTGACT TAGCCGTGCC GCCCGTGGCC 
AGTCTGCCTC CCAGCACCCT GGACGGGCCT CTCTCATCTT CTCAGGAGAG CAGCTTTCAC 
GGGAACACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGCCCCA GACGCCTTCG 
AGTGAGCACG CCTGTGAGGG GGACTACGTC AGTCTGTATT GCTCTGGCCA GAGCAGCGAG 
GAGCTGGCCC GCCCTCGAGG AGAGTCACCC GCGGCGAAGG ATGGACACTC CAGAGACGCC 
ACATCGGCCA GCTGTGTGCC CGGGAAGGAG GGCAGGGACA GCGGGGACAG GGCCCGGAAG 
TCACCAGACG CTCCAGAGGC TCAGTCAGAG GAGGAGGTGG ACGAGCTCTC CCTCATCGAC 
CACAACGAGA TCATGGCGAG GCTGACCCTC AAGCAAGAGG GCGATGACGG GCCAGACGTC 
CGTGGAGGGT CGGGCGACAT CCTCCTCGTC CATGCCACGG AGACGGACCG GAAAGATCTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCACTCC TGAGGAGCTC 
ATCCAGAAGC TGCAGTACAG ATACGAGAAG TTCTCACCTT TCGCCGACAC ATTCAAGAAG 
CGTGTGAGCA AGAACACGTT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTA 
GAGCTGACAG AAGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT GGTGCGCAGC 
GGGGAGCTCA GCCTAGCACG CGTGCTCCGC AAGAACATTC TGGACAAGGT CGACCAGAAG 
AAGCTGCTCC GGTGCGCCAA CTCTGACCAG CCCCTGGCGG CCCGGGGAGT GGCAGCCAGG 
CCCGGGACCT TGCACGACTT CCACAGCCAT GAAATAGCCG AGCAGCTGAC TCTGCTAGAT 
GCCGAGCTCT TTTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCTAA AGAGCAGAAC 
GAGGAGAAGA GCCCGAACCT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCGA TAATCATGTT GCAGGAAAAG GCCCAAGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTACGGAAG CTGAACAACT TCAACTCCTA CTTGGCCATC 
CTCTCGGCGC TGGACTCGGC CCCCATTCGC AGGCTGGAGT GGCAGAAACA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATCGAC AGCTCGTCAT CCTTCCGCGC CTACCGGGCC 
GCCCTCTCGG AGGTAGAACC GCCCTGCATC CCATACCTGG GGCTCATCCT GCAGGACCTC 
ACCTTCGTCC ACCTGGGGAA CCCAGACTAT ATTGACGGGA AGGTGAACTT CTCCAAACGC 
TGGCAGCAGT TCAACATCCT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTACGACATC 
CGGAGAAATG ACGACATCAT TAACTTCTTC AACGACTTCA GTGACCACCT GGCCGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATCAAA CCCAGGAACA TAACGAGGAG GAAGACAGAT 
AGAGAAGAAA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_010810419.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808721.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

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CloneID	OBa50253
Clone ID Related Accession (Same CDS sequence)	XM_010810419.1
Accession Version	XM_010810419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCATCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TACCTGTGCC CTCGTACAAG TCCGTGTTTA GGTCCTACTC GCAGGATTTC 
GTGCCTCACA GCCAAGCTTC CGTCCAGCCT TTCCTTCCGT CTACCTCCTC TTCCTCTCCA 
CACTTCCCAC CTGTCCACCA GTATCAGAGC TCTGACTTAG CCGTGCCGCC CGTGGCCAGT 
CTGCCTCCCA GCACCCTGGA CGGGCCTCTC TCATCTTCTC AGGAGAGCAG CTTTCACGGG 
AACACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGCCCCAGAC GCCTTCGAGT 
GAGCACGCCT GTGAGGGGGA CTACGTCAGT CTGTATTGCT CTGGCCAGAG CAGCGAGGAG 
CTGGCCCGCC CTCGAGGAGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808721.1
CDS	14..3808
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_010810419.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810419.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGAAGGACAA ATTCCACTCG 
CCCAAAATCA AGAGGACGCC ATCAAAGAAG GGAAAACCAG CCGAGGTGTC TGTGAAGATT 
CCAGAGAAGC CCGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAG TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCTATTG ATAAGAAGGT CCTGGAGATG 
CTCCCAGGGT CCGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAGCGACCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCCTCC TGCTACAGCC GAGTGTACCA AAGTCTCGCC 
AATCTCATCC GTTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGATAAAGAG 
ATGGTGACAA CTGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGACTCACCA TCGAGAAGCA GGGGCATCCA TCTCCAACAA GCCCTGTGAA ACCCAGCTCC 
CCAGCCTGCA AACCTGACAG CCAGTCTGAG CTCCCCCTGA CGGATCGAGA GATGGAGATA 
CTAAACAAGA CGACCAGCCT GTCACAGTCA ACCGAGGGGC TCCAAGACTC TATGGACGAA 
GAGGTTGCAC CCCCCAAGCC CCCCTTACCT GGCATTAGGG TGGTTGATAA CAGTCCTCCA 
CCAGCACTGC CCCCGAAGAA GAGACAGTCG GCTCCGTCCC CCACGCGTGT GGCCGTTGTG 
GCCCCCATGA GTCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATTAA TAGGCAGGAC 
TTTGACGTGG ACTGTTACGC ACAGAGACGG CTGTCGGGAG GCAGCCACTC GTACAGCGGT 
GAGTCTCCCC GCCTGTCGCC CTGCAGCAGC ATCGGCAAGC TCAGCAAGTC GGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGCGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GATCATTACG ATCCTGACTA CGAGTTCCTT CAGCAAGACC TCTCCAACGC AGACCAGATA 
CCTCAGCAAG TGGCCTGTAA CCTCAGCCCG CTGCCAGAGT CCTTGGGGGA GTCTGGGTCT 
CCCTTTCTTG GCCATCCTCT CCAGTTGCCT CTGGGCAGCT GTCCCCAGCC AGATGGGTCC 
TCAGCCCCGG GGCAGCAGAC GGACGTGCCG CCAGCCCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACCTTGGA CAGCTCTGGC TGCCGGGCGT CCTACGAGCG ACACCCCTCA 
CAGTACGACA ACATCTCCGA GGATGACCTG CAGAACCCAG CCTCCGTCCA GTCCGTCCCC 
TTCACGCCCT TTGCTGCTAT TCTTCCCTTT CAGCAAGGAG GTTCCTCAGC CTCTGTCGAC 
TTCATGGGTG ATTTCACTGT TTCTGAATCA ACGGGTGACC CAGAAAAACC GCCACCTCTC 
CCCGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTTGAGGA CTACTCGGAG 
CCACAGCCTT CCATGTTCTA CCAGACGCCG CAGAAGGAAC ACGTGTACCA GCAGAAGAAC 
AAGCTTCTCA TGGAGGTGTA TGGCTTCAAC GACTCCTTCA GCACCGGCGA CGCCGCACAC 
GATCTCGCCC CGCCCCCCGC CCTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCATCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TACCTGTGCC CTCGTACAAG TCCGTGTTTA GGTCCTACTC GCAGGATTTC 
GTGCCTCACA GCCAAGCTTC CGTCCAGCCT TTCCTTCCGT CTACCTCCTC TTCCTCTCCA 
CACTTCCCAC CTGTCCACCA GTATCAGAGC TCTGACTTAG CCGTGCCGCC CGTGGCCAGT 
CTGCCTCCCA GCACCCTGGA CGGGCCTCTC TCATCTTCTC AGGAGAGCAG CTTTCACGGG 
AACACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGCCCCAGAC GCCTTCGAGT 
GAGCACGCCT GTGAGGGGGA CTACGTCAGT CTGTATTGCT CTGGCCAGAG CAGCGAGGAG 
CTGGCCCGCC CTCGAGGAGA GTCACCCGCG GCGAAGGATG GACACTCCAG AGACGCCACA 
TCGGCCAGCT GTGTGCCCGG GAAGGAGGGC AGGGACAGCG GGGACAGGGC CCGGAAGTCA 
CCAGACGCTC CAGAGGCTCA GTCAGAGGAG GAGGTGGACG AGCTCTCCCT CATCGACCAC 
AACGAGATCA TGGCGAGGCT GACCCTCAAG CAAGAGGGCG ATGACGGGCC AGACGTCCGT 
GGAGGGTCGG GCGACATCCT CCTCGTCCAT GCCACGGAGA CGGACCGGAA AGATCTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCACTCCTGA GGAGCTCATC 
CAGAAGCTGC AGTACAGATA CGAGAAGTTC TCACCTTTCG CCGACACATT CAAGAAGCGT 
GTGAGCAAGA ACACGTTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTAGAG 
CTGACAGAAG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTGGT GCGCAGCGGG 
GAGCTCAGCC TAGCACGCGT GCTCCGCAAG AACATTCTGG ACAAGGTCGA CCAGAAGAAG 
CTGCTCCGGT GCGCCAACTC TGACCAGCCC CTGGCGGCCC GGGGAGTGGC AGCCAGGCCC 
GGGACCTTGC ACGACTTCCA CAGCCATGAA ATAGCCGAGC AGCTGACTCT GCTAGATGCC 
GAGCTCTTTT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCTAAAGA GCAGAACGAG 
GAGAAGAGCC CGAACCTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCGATAA TCATGTTGCA GGAAAAGGCC CAAGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT ACGGAAGCTG AACAACTTCA ACTCCTACTT GGCCATCCTC 
TCGGCGCTGG ACTCGGCCCC CATTCGCAGG CTGGAGTGGC AGAAACAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATCGACAGC TCGTCATCCT TCCGCGCCTA CCGGGCCGCC 
CTCTCGGAGG TAGAACCGCC CTGCATCCCA TACCTGGGGC TCATCCTGCA GGACCTCACC 
TTCGTCCACC TGGGGAACCC AGACTATATT GACGGGAAGG TGAACTTCTC CAAACGCTGG 
CAGCAGTTCA ACATCCTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA CGACATCCGG 
AGAAATGACG ACATCATTAA CTTCTTCAAC GACTTCAGTG ACCACCTGGC CGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATCAAACCC AGGAACATAA CGAGGAGGAA GACAGATAGA 
GAAGAAAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_010810421.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808723.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa50254
Clone ID Related Accession (Same CDS sequence)	XM_010810421.2 , XM_015473631.1 , XM_015473629.1 , XM_015473630.1
Accession Version	XM_010810421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808723.1
CDS	313..4014
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_010810421.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810421.2

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473631.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329117.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa50254
Clone ID Related Accession (Same CDS sequence)	XM_010810421.2 , XM_015473631.1 , XM_015473629.1 , XM_015473630.1
Accession Version	XM_015473631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329117.1
CDS	430..4131
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473631.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473631.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473629.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329115.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa50254
Clone ID Related Accession (Same CDS sequence)	XM_010810421.2 , XM_015473631.1 , XM_015473629.1 , XM_015473630.1
Accession Version	XM_015473629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329115.1
CDS	888..4589
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473629.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473629.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015473630.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329116.1 Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa50254
Clone ID Related Accession (Same CDS sequence)	XM_010810421.2 , XM_015473631.1 , XM_015473629.1 , XM_015473630.1
Accession Version	XM_015473630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329116.1
CDS	280..3981
Translation	MKLKDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGHPSPTSPVKPSSPACKPDSQSELPLTDREMEILNKTTSLSQSTEGLQDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYSGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQVACNLSPLPESLGESGSPFLGHPLQLPLGSCPQPDGSSAPGQQTDVPPALPEKKRRSAASQTLDSSGCRASYERHPSQYDNISEDDLQNPASVQSVPFTPFAAILPFQQGGSSASVDFMGDFTVSESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQKEHVYQQKNKLLMEVYGFNDSFSTGDAAHDLAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGISVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHSQASVQPFLPSTSSSSPHFPPVHQYQSSDLAVPPVASLPPSTLDGPLSSSQESSFHGNTVCLPSETSFTDSPQTPSSEHACEGDYVSLYCSGQSSEELARPRGESPAAKDGHSRDATSASCVPGKEGRDSGDRARKSPDAPEAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIQKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVRSGELSLARVLRKNILDKVDQKKLLRCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015473630.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473630.1

ORF Insert Sequence:

ATGAAGCTGA AGGACAAATT CCACTCGCCC AAAATCAAGA GGACGCCATC AAAGAAGGGA 
AAACCAGCCG AGGTGTCTGT GAAGATTCCA GAGAAGCCCG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTATCCCC TTGGATCTGG AGCAGCAGGC AGTAGAGTTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCTATTGATA AGAAGGTCCT GGAGATGCTC CCAGGGTCCG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAG CGACCCTCGA ATTCAGCACA GCTCAGCCCT CTCCTCCTGC 
TACAGCCGAG TGTACCAAAG TCTCGCCAAT CTCATCCGTT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA TAAAGAGATG GTGACAACTG TGAAGGGGGT CATCAAGGCC 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGA CTCACCATCG AGAAGCAGGG GCATCCATCT 
CCAACAAGCC CTGTGAAACC CAGCTCCCCA GCCTGCAAAC CTGACAGCCA GTCTGAGCTC 
CCCCTGACGG ATCGAGAGAT GGAGATACTA AACAAGACGA CCAGCCTGTC ACAGTCAACC 
GAGGGGCTCC AAGACTCTAT GGACGAAGAG GTTGCACCCC CCAAGCCCCC CTTACCTGGC 
ATTAGGGTGG TTGATAACAG TCCTCCACCA GCACTGCCCC CGAAGAAGAG ACAGTCGGCT 
CCGTCCCCCA CGCGTGTGGC CGTTGTGGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATTAATAG GCAGGACTTT GACGTGGACT GTTACGCACA GAGACGGCTG 
TCGGGAGGCA GCCACTCGTA CAGCGGTGAG TCTCCCCGCC TGTCGCCCTG CAGCAGCATC 
GGCAAGCTCA GCAAGTCGGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTACGATC CTGACTACGA GTTCCTTCAG 
CAAGACCTCT CCAACGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT CAGCCCGCTG 
CCAGAGTCCT TGGGGGAGTC TGGGTCTCCC TTTCTTGGCC ATCCTCTCCA GTTGCCTCTG 
GGCAGCTGTC CCCAGCCAGA TGGGTCCTCA GCCCCGGGGC AGCAGACGGA CGTGCCGCCA 
GCCCTGCCCG AGAAGAAGCG CAGGAGTGCA GCCTCCCAGA CCTTGGACAG CTCTGGCTGC 
CGGGCGTCCT ACGAGCGACA CCCCTCACAG TACGACAACA TCTCCGAGGA TGACCTGCAG 
AACCCAGCCT CCGTCCAGTC CGTCCCCTTC ACGCCCTTTG CTGCTATTCT TCCCTTTCAG 
CAAGGAGGTT CCTCAGCCTC TGTCGACTTC ATGGGTGATT TCACTGTTTC TGAATCAACG 
GGTGACCCAG AAAAACCGCC ACCTCTCCCC GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGCTGC TTGAGGACTA CTCGGAGCCA CAGCCTTCCA TGTTCTACCA GACGCCGCAG 
AAGGAACACG TGTACCAGCA GAAGAACAAG CTTCTCATGG AGGTGTATGG CTTCAACGAC 
TCCTTCAGCA CCGGCGACGC CGCACACGAT CTCGCCCCGC CCCCCGCCCT GCCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CATCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTAC CTGTGCCCTC GTACAAGTCC 
GTGTTTAGGT CCTACTCGCA GGATTTCGTG CCTCACAGCC AAGCTTCCGT CCAGCCTTTC 
CTTCCGTCTA CCTCCTCTTC CTCTCCACAC TTCCCACCTG TCCACCAGTA TCAGAGCTCT 
GACTTAGCCG TGCCGCCCGT GGCCAGTCTG CCTCCCAGCA CCCTGGACGG GCCTCTCTCA 
TCTTCTCAGG AGAGCAGCTT TCACGGGAAC ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGC CCCAGACGCC TTCGAGTGAG CACGCCTGTG AGGGGGACTA CGTCAGTCTG 
TATTGCTCTG GCCAGAGCAG CGAGGAGCTG GCCCGCCCTC GAGGAGAGTC ACCCGCGGCG 
AAGGATGGAC ACTCCAGAGA CGCCACATCG GCCAGCTGTG TGCCCGGGAA GGAGGGCAGG 
GACAGCGGGG ACAGGGCCCG GAAGTCACCA GACGCTCCAG AGGCTCAGTC AGAGGAGGAG 
GTGGACGAGC TCTCCCTCAT CGACCACAAC GAGATCATGG CGAGGCTGAC CCTCAAGCAA 
GAGGGCGATG ACGGGCCAGA CGTCCGTGGA GGGTCGGGCG ACATCCTCCT CGTCCATGCC 
ACGGAGACGG ACCGGAAAGA TCTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCA CTCCTGAGGA GCTCATCCAG AAGCTGCAGT ACAGATACGA GAAGTTCTCA 
CCTTTCGCCG ACACATTCAA GAAGCGTGTG AGCAAGAACA CGTTCTTCGT GCTGGTGCGG 
GTGGTGGACG AGCTCTGCCT GGTAGAGCTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAG 
CTGGTCTTCC GCCTGGTGCG CAGCGGGGAG CTCAGCCTAG CACGCGTGCT CCGCAAGAAC 
ATTCTGGACA AGGTCGACCA GAAGAAGCTG CTCCGGTGCG CCAACTCTGA CCAGCCCCTG 
GCGGCCCGGG GAGTGGCAGC CAGGCCCGGG ACCTTGCACG ACTTCCACAG CCATGAAATA 
GCCGAGCAGC TGACTCTGCT AGATGCCGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CTAAAGAGCA GAACGAGGAG AAGAGCCCGA ACCTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCGATAATCA TGTTGCAGGA AAAGGCCCAA 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC 
AACTTCAACT CCTACTTGGC CATCCTCTCG GCGCTGGACT CGGCCCCCAT TCGCAGGCTG 
GAGTGGCAGA AACAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT CGACAGCTCG 
TCATCCTTCC GCGCCTACCG GGCCGCCCTC TCGGAGGTAG AACCGCCCTG CATCCCATAC 
CTGGGGCTCA TCCTGCAGGA CCTCACCTTC GTCCACCTGG GGAACCCAGA CTATATTGAC 
GGGAAGGTGA ACTTCTCCAA ACGCTGGCAG CAGTTCAACA TCCTGGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTACGA CATCCGGAGA AATGACGACA TCATTAACTT CTTCAACGAC 
TTCAGTGACC ACCTGGCCGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT CAAACCCAGG 
AACATAACGA GGAGGAAGAC AGATAGAGAA GAAAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RAPGEF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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