Rapgef1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Rapgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa15511 XM_006233902.3
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa15511 XM_006224337.3
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa43541 XM_008775310.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa43542 XM_008775311.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa43543 XM_008775312.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa43544 XM_008775313.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa43545 XM_008775314.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa55077 XM_017601886.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55078 XM_017601887.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55079 XM_017601888.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55079 XM_017592102.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55080 XM_017601889.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55081 XM_017601890.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55082 XM_017601891.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55083 XM_017601892.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa55084 XM_017601893.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa60565 XM_017592103.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60566 XM_008761683.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60567 XM_017592104.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60568 XM_017592105.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60569 XM_008761684.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60570 XM_008761685.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60571 XM_017592106.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60572 XM_008761686.2
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa60573 XM_017592107.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa60574 XM_017592108.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa60575 XM_017592109.1
Latest version!
Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa15511
    Clone ID Related Accession (Same CDS sequence) XM_006233902.3 , XM_006224337.3
    Accession Version XM_006233902.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006233964.1
    CDS3179..6433
    Translation

    Target ORF information:

    RefSeq Version XM_006233902.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006233902.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa15511
    Clone ID Related Accession (Same CDS sequence) XM_006233902.3 , XM_006224337.3
    Accession Version XM_006224337.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006224337.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006224399.1
    CDS3179..6433
    Translation

    Target ORF information:

    RefSeq Version XM_006224337.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006224337.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43541
    Clone ID Related Accession (Same CDS sequence) XM_008775310.2
    Accession Version XM_008775310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGAGTG AAAACGCCAG CAGTGAGGAA
    GCTGGTGGGG GTGAATATGT CAATCTGTAC TCCTCTGGCC AGACCAGCGA GGAGCTAGCT
    CCCTCTGGAG GAGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773532.1
    CDS16..3864
    Translation

    Target ORF information:

    RefSeq Version XM_008775310.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGAGTG AAAACGCCAG CAGTGAGGAA
    GCTGGTGGGG GTGAATATGT CAATCTGTAC TCCTCTGGCC AGACCAGCGA GGAGCTAGCT
    CCCTCTGGAG GAGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43542
    Clone ID Related Accession (Same CDS sequence) XM_008775311.2
    Accession Version XM_008775311.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGGAAC CGCCATCTGG GAAGGACGGA
    CATCCCAGAG ATCTTTCGGT CAGCAGTGCA TCTGGGAAGG ACAGCAGAGA AAATGGGGAG
    AGGTCCCCGA AGTCACTGGA TGCTCTGGAG TCAGCCCAGT CAGAAGAGGA AGTGGATGAA
    CTGTCCCTCA TTGACCACAA TGAAATCATG GCCAGGCTGA CACTCAAGCA AGAGGGTGAT
    GATGGACCAG ATGTTCGTGG AGGTTCAGGA GACATCTTAC TGGTCCATGC TACTGAGACA
    GACAGAAAAG ACCTGGTCCT GTACTGTGAA GCCTTCCTGA CCACCTACAG AACCTTCATC
    AGCCCCGAGG AGCTCATCAA GAAGCTGCAG TGCCGGTACG AGAAGTTCTC TCCCTTTGCT
    GACACATTCA AGAAGCGAGT GAGCAAGAAC ACATTCTTCG TGCTGGTGCG GGTGGTGGAC
    GAGCTCTGCC TGGTGGAGCT GACAGAGGAG ATCCTGAAGC TGCTGATGGA GCTAGTCTTC
    CGCCTAGTGT GCAGTGGAGA GCTCAGCTTG GCCAGGGTAC TCCGAAAGAA CATTCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCCATTCCG ACCAGCCTTT GGCAGCCAGG
    GGTGTTGCAG CCAGGCCAGG AACCTTGCAT GATTTCCACA GCCACGAGAT AGCTGAACAG
    CTGACACTGC TGGATGCCGA GCTTTTCTAT AAGATAGAGA TTCCCGAAGT TTTGCTTTGG
    GCCAAAGAGC AGAATGAAGA GAAGAGCCCC AATCTGACCC AGTTCACAGA GCACTTCAAC
    AACATGTCCT ACTGGGTGCG GTCCGTCATC ATGCTGCAGG AGAAGGCCCA GGACCGGGAG
    AGGCTGCTCC TGAAGTTCAT CAAGATCATG AAGCACCTGC GCAAGCTCAA CAATTTCAAC
    TCCTACCTGG CCATCCTCTC CGCACTGGAC TCGGCCCCCA TCCGCAGACT GGAGTGGCAG
    CGACAGACCT CAGAGGGCCT GGCTGAGTAC TGCACGTTGA TTGACAGCTC ATCCTCCTTC
    CGAGCCTACC GGGCTGCCCT CTCAGAGGTG GAACCTCCAT GTATCCCATA CCTAGGTTTG
    ATCCTGCAAG ACCTGACGTT CGTCCACCTG GGAAACCCAG ACTACATAGA CGGGAAGGTC
    AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGCTG TTTCCAGCAG
    GTGCACTATG AAATCCGGAG AAACGACGAC ATCATAAACT TCTTCAATGA CTTCAGCGAC
    CACCTGGCCG AGGAAGCCCT ATGGGAACTC TCTCTGAAGA TCAAGCCTAG GAACATAACA
    AGGCGGAAAA CAGACCGTGA AGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773533.1
    CDS16..3768
    Translation

    Target ORF information:

    RefSeq Version XM_008775311.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775311.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGCAGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGGAAC CGCCATCTGG GAAGGACGGA
    CATCCCAGAG ATCTTTCGGT CAGCAGTGCA TCTGGGAAGG ACAGCAGAGA AAATGGGGAG
    AGGTCCCCGA AGTCACTGGA TGCTCTGGAG TCAGCCCAGT CAGAAGAGGA AGTGGATGAA
    CTGTCCCTCA TTGACCACAA TGAAATCATG GCCAGGCTGA CACTCAAGCA AGAGGGTGAT
    GATGGACCAG ATGTTCGTGG AGGTTCAGGA GACATCTTAC TGGTCCATGC TACTGAGACA
    GACAGAAAAG ACCTGGTCCT GTACTGTGAA GCCTTCCTGA CCACCTACAG AACCTTCATC
    AGCCCCGAGG AGCTCATCAA GAAGCTGCAG TGCCGGTACG AGAAGTTCTC TCCCTTTGCT
    GACACATTCA AGAAGCGAGT GAGCAAGAAC ACATTCTTCG TGCTGGTGCG GGTGGTGGAC
    GAGCTCTGCC TGGTGGAGCT GACAGAGGAG ATCCTGAAGC TGCTGATGGA GCTAGTCTTC
    CGCCTAGTGT GCAGTGGAGA GCTCAGCTTG GCCAGGGTAC TCCGAAAGAA CATTCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCCATTCCG ACCAGCCTTT GGCAGCCAGG
    GGTGTTGCAG CCAGGCCAGG AACCTTGCAT GATTTCCACA GCCACGAGAT AGCTGAACAG
    CTGACACTGC TGGATGCCGA GCTTTTCTAT AAGATAGAGA TTCCCGAAGT TTTGCTTTGG
    GCCAAAGAGC AGAATGAAGA GAAGAGCCCC AATCTGACCC AGTTCACAGA GCACTTCAAC
    AACATGTCCT ACTGGGTGCG GTCCGTCATC ATGCTGCAGG AGAAGGCCCA GGACCGGGAG
    AGGCTGCTCC TGAAGTTCAT CAAGATCATG AAGCACCTGC GCAAGCTCAA CAATTTCAAC
    TCCTACCTGG CCATCCTCTC CGCACTGGAC TCGGCCCCCA TCCGCAGACT GGAGTGGCAG
    CGACAGACCT CAGAGGGCCT GGCTGAGTAC TGCACGTTGA TTGACAGCTC ATCCTCCTTC
    CGAGCCTACC GGGCTGCCCT CTCAGAGGTG GAACCTCCAT GTATCCCATA CCTAGGTTTG
    ATCCTGCAAG ACCTGACGTT CGTCCACCTG GGAAACCCAG ACTACATAGA CGGGAAGGTC
    AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGCTG TTTCCAGCAG
    GTGCACTATG AAATCCGGAG AAACGACGAC ATCATAAACT TCTTCAATGA CTTCAGCGAC
    CACCTGGCCG AGGAAGCCCT ATGGGAACTC TCTCTGAAGA TCAAGCCTAG GAACATAACA
    AGGCGGAAAA CAGACCGTGA AGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43543
    Clone ID Related Accession (Same CDS sequence) XM_008775312.2
    Accession Version XM_008775312.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TCCACCAGCA TTACCACCCA AGAAAAGGCA ATCTGCTCCA
    TCCCCTACCC GGGTGGCTGT GGTAGCCCCC ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG
    CCTGTTGGAA TCAATAGGCA GGACTTTGAT GTTGAATGTT ACACCCAGAG GCGCCTGTCA
    GGAGGCAGCC GCTCCTGTGG TGGTGAATCT CCTCGCCTGT CCCCCTGCAG CAGTACAGGC
    AAGCTCAGCC GGTCAGACGA GCAGCTGTCC TCCCTAGACA GGGATAGCGG GCAGTGCTCA
    CGGAACACAA GCTGTGAAAC ACTAGATCAC TATGACCCCG ACTATGAATT CCTCCAGCAA
    GACCTCTCCA ATGCAGACCA GACCCCTCCA CAGGCAGCCT GTAACCTCAG CCCACTGCCG
    GAGTCCATGG GGGAGTCTGG GCCCCCATTT GTTGGCTACT CTTTCCAGCT GCCTCTGGGC
    AGCTGTCTCC AGCAGGATGG ACAGCAGACA GATACTCCAC CTGCCCTTCC TGAGAAGAAG
    CGTAGGAGCG CAGTCTCCCA GACCACGGAC AGCTCTGGCT GCAGGGTGTC CTATGAGCGA
    CACCCCTCAC AGTATGACAA CATCTCAGAA GATGATCTGC AGAACCCAGC CCAGCCTGTG
    TCCTACCCGC CCTTTGCTGC TATCCTGCCC TTTCAGCAGG GAGCTTCTTC CACCCCTGTT
    GAGTTTGTGG GTGATTTCAG TGTTCCTGAG TCAGCCGGTG ACCCAGAAAA GCCACCTCCT
    CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AACTGCTGGA GGACTACTCA
    GAGCCACAGC CCTCCATGTT CTACCAGACA CCGCAGAGTG AGCACATCTA CCAGCAGAAG
    AACAAGATGC TCATGGAGGT CTATGGCTTC AGCGACTCCT TCTGTGGTAG TGACTCCACG
    CAGGAGCTGG CCCCTCCACC CGCTTTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT
    GCTGCTTCTT CCTTCTCTGT CTCCCACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC
    GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTATCCGTGT CTAACTCCTT TCTCAACCGG
    CACGGCAGCT TGCCTGTGCC CTCGTACAAA TCTGTGTTTA GGTCTTACTC CCAGGACTTC
    ATGCCTCACC ACCAAGCCTC CGTCCAACCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA
    CATTTCCCAC CTGTCCACGC GTCCCAGAGC TCGGACTTAG CGGTGCCCAC CGTAAGCAGT
    CCGCCTCCCA GCACCGTGGA CGGGCCTCTC TCGTCTTCTC AGGACAGCAG CTTTCATGGG
    AACCCTGTCC GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGCAGT
    GAGGAAGCTG GTGGGGGTGA ATATGTCAAT CTGTACTCCT CTGGCCAGAC CAGCGAGGAG
    CTAGCTCCCT CTGGAGGAGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773534.1
    CDS16..3750
    Translation

    Target ORF information:

    RefSeq Version XM_008775312.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TCCACCAGCA TTACCACCCA AGAAAAGGCA ATCTGCTCCA
    TCCCCTACCC GGGTGGCTGT GGTAGCCCCC ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG
    CCTGTTGGAA TCAATAGGCA GGACTTTGAT GTTGAATGTT ACACCCAGAG GCGCCTGTCA
    GGAGGCAGCC GCTCCTGTGG TGGTGAATCT CCTCGCCTGT CCCCCTGCAG CAGTACAGGC
    AAGCTCAGCC GGTCAGACGA GCAGCTGTCC TCCCTAGACA GGGATAGCGG GCAGTGCTCA
    CGGAACACAA GCTGTGAAAC ACTAGATCAC TATGACCCCG ACTATGAATT CCTCCAGCAA
    GACCTCTCCA ATGCAGACCA GACCCCTCCA CAGGCAGCCT GTAACCTCAG CCCACTGCCG
    GAGTCCATGG GGGAGTCTGG GCCCCCATTT GTTGGCTACT CTTTCCAGCT GCCTCTGGGC
    AGCTGTCTCC AGCAGGATGG ACAGCAGACA GATACTCCAC CTGCCCTTCC TGAGAAGAAG
    CGTAGGAGCG CAGTCTCCCA GACCACGGAC AGCTCTGGCT GCAGGGTGTC CTATGAGCGA
    CACCCCTCAC AGTATGACAA CATCTCAGAA GATGATCTGC AGAACCCAGC CCAGCCTGTG
    TCCTACCCGC CCTTTGCTGC TATCCTGCCC TTTCAGCAGG GAGCTTCTTC CACCCCTGTT
    GAGTTTGTGG GTGATTTCAG TGTTCCTGAG TCAGCCGGTG ACCCAGAAAA GCCACCTCCT
    CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AACTGCTGGA GGACTACTCA
    GAGCCACAGC CCTCCATGTT CTACCAGACA CCGCAGAGTG AGCACATCTA CCAGCAGAAG
    AACAAGATGC TCATGGAGGT CTATGGCTTC AGCGACTCCT TCTGTGGTAG TGACTCCACG
    CAGGAGCTGG CCCCTCCACC CGCTTTGCCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT
    GCTGCTTCTT CCTTCTCTGT CTCCCACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC
    GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTATCCGTGT CTAACTCCTT TCTCAACCGG
    CACGGCAGCT TGCCTGTGCC CTCGTACAAA TCTGTGTTTA GGTCTTACTC CCAGGACTTC
    ATGCCTCACC ACCAAGCCTC CGTCCAACCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA
    CATTTCCCAC CTGTCCACGC GTCCCAGAGC TCGGACTTAG CGGTGCCCAC CGTAAGCAGT
    CCGCCTCCCA GCACCGTGGA CGGGCCTCTC TCGTCTTCTC AGGACAGCAG CTTTCATGGG
    AACCCTGTCC GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGCAGT
    GAGGAAGCTG GTGGGGGTGA ATATGTCAAT CTGTACTCCT CTGGCCAGAC CAGCGAGGAG
    CTAGCTCCCT CTGGAGGAGA ACCGCCATCT GGGAAGGACG GACATCCCAG AGATCTTTCG
    GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AGAGGTCCCC GAAGTCACTG
    GATGCTCTGG AGTCAGCCCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC
    AATGAAATCA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGACC AGATGTTCGT
    GGAGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTC
    CTGTACTGTG AAGCCTTCCT GACCACCTAC AGAACCTTCA TCAGCCCCGA GGAGCTCATC
    AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACATT CAAGAAGCGA
    GTGAGCAAGA ACACATTCTT CGTGCTGGTG CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG
    CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA
    GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG
    CTGCTCAGGT GTGCCCATTC CGACCAGCCT TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA
    GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAAC AGCTGACACT GCTGGATGCC
    GAGCTTTTCT ATAAGATAGA GATTCCCGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAA
    GAGAAGAGCC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG
    CGGTCCGTCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTGAAGTTC
    ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAATTTCA ACTCCTACCT GGCCATCCTC
    TCCGCACTGG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGACAGAC CTCAGAGGGC
    CTGGCTGAGT ACTGCACGTT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCC
    CTCTCAGAGG TGGAACCTCC ATGTATCCCA TACCTAGGTT TGATCCTGCA AGACCTGACG
    TTCGTCCACC TGGGAAACCC AGACTACATA GACGGGAAGG TCAACTTCTC CAAGCGGTGG
    CAGCAGTTCA ACATCCTGGA CAGCATGCGC TGTTTCCAGC AGGTGCACTA TGAAATCCGG
    AGAAACGACG ACATCATAAA CTTCTTCAAT GACTTCAGCG ACCACCTGGC CGAGGAAGCC
    CTATGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGT
    GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43544
    Clone ID Related Accession (Same CDS sequence) XM_008775313.2
    Accession Version XM_008775313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TCCACCAGCA TTACCACCCA AGAAAAGGCA ATCTGCTCCA
    TCCCCTACCC GGGTGGCTGT GGTAGCCCCC ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG
    CCTGTTGGAA TCAATAGGCA GGACTTTGAT GTTGAATGTT ACACCCAGAG GCGCCTGTCA
    GGAGGCAGCC GCTCCTGTGG TGGTGAATCT CCTCGCCTGT CCCCCTGCAG CAGTACAGGC
    AAGCTCAGCC GGTCAGACGA GCAGCTGTCC TCCCTAGACA GGGATAGCGG GCAGTGCTCA
    CGGAACACAA GCTGTGAAAC ACTAGATCAC TATGACCCCG ACTATGAATT CCTCCAGCAA
    GACCTCTCCA ATGCAGACCA GACCCCTCCA CAGGCAGCCT GTAACCTCAG CCCACTGCCG
    GAGTCCATGG GGGAGTCTGG GCCCCCATTT GTTGGCTACT CTTTCCAGCT GCCTCTGGGC
    AGCTGTCTCC AGCAGGATGG ACAGCAGACA GATACTCCAC CTGCCCTTCC TGAGAAGAAG
    CGTAGGAGCG CAGTCTCCCA GACCACGGAC AGCTCTGGCT GCAGGGTGTC CTATGAGCGA
    CACCCCTCAC AGTATGACAA CATCTCAGAA GATGATCTGC AGAACCCAGC CCAGCCTGTG
    TCCTACCCGC CCTTTGCTGC TATCCTGCCC TTTCAGCAGG GAGCTTCTTC CACCCCTGTT
    GAGTTTGTGG GTGATTTCAG TGTTCCTGAG TCAGCCGGTG ACCCAGAAAA GCCACCTCCT
    CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AACTGCTGGA GGACTACTCA
    GAGCCACAGC CCTCCATGTT CTACCAGACA CCGCAGAGTG AGCACATCTA CCAGCAGAAG
    AACAAGATGC TCATGGAGGT CTATGGCTTC AGCGACTCCT TCTGTGGTAG TGACTCCACG
    CAGGAGCTGG CCCCTCCACC CGCTTTGCCC CCCAAGCAGC GGCAGCTGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773535.1
    CDS16..3651
    Translation

    Target ORF information:

    RefSeq Version XM_008775313.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TCCACCAGCA TTACCACCCA AGAAAAGGCA ATCTGCTCCA
    TCCCCTACCC GGGTGGCTGT GGTAGCCCCC ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG
    CCTGTTGGAA TCAATAGGCA GGACTTTGAT GTTGAATGTT ACACCCAGAG GCGCCTGTCA
    GGAGGCAGCC GCTCCTGTGG TGGTGAATCT CCTCGCCTGT CCCCCTGCAG CAGTACAGGC
    AAGCTCAGCC GGTCAGACGA GCAGCTGTCC TCCCTAGACA GGGATAGCGG GCAGTGCTCA
    CGGAACACAA GCTGTGAAAC ACTAGATCAC TATGACCCCG ACTATGAATT CCTCCAGCAA
    GACCTCTCCA ATGCAGACCA GACCCCTCCA CAGGCAGCCT GTAACCTCAG CCCACTGCCG
    GAGTCCATGG GGGAGTCTGG GCCCCCATTT GTTGGCTACT CTTTCCAGCT GCCTCTGGGC
    AGCTGTCTCC AGCAGGATGG ACAGCAGACA GATACTCCAC CTGCCCTTCC TGAGAAGAAG
    CGTAGGAGCG CAGTCTCCCA GACCACGGAC AGCTCTGGCT GCAGGGTGTC CTATGAGCGA
    CACCCCTCAC AGTATGACAA CATCTCAGAA GATGATCTGC AGAACCCAGC CCAGCCTGTG
    TCCTACCCGC CCTTTGCTGC TATCCTGCCC TTTCAGCAGG GAGCTTCTTC CACCCCTGTT
    GAGTTTGTGG GTGATTTCAG TGTTCCTGAG TCAGCCGGTG ACCCAGAAAA GCCACCTCCT
    CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AACTGCTGGA GGACTACTCA
    GAGCCACAGC CCTCCATGTT CTACCAGACA CCGCAGAGTG AGCACATCTA CCAGCAGAAG
    AACAAGATGC TCATGGAGGT CTATGGCTTC AGCGACTCCT TCTGTGGTAG TGACTCCACG
    CAGGAGCTGG CCCCTCCACC CGCTTTGCCC CCCAAGCAGC GGCAGCTGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43545
    Clone ID Related Accession (Same CDS sequence) XM_008775314.2
    Accession Version XM_008775314.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGGAACCGCC ATCTGGGAAG
    GACGGACATC CCAGAGATCT TTCGGTCAGC AGTGCATCTG GGAAGGACAG CAGAGAAAAT
    GGGGAGAGGT CCCCGAAGTC ACTGGATGCT CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG
    GATGAACTGT CCCTCATTGA CCACAATGAA ATCATGGCCA GGCTGACACT CAAGCAAGAG
    GGTGATGATG GACCAGATGT TCGTGGAGGT TCAGGAGACA TCTTACTGGT CCATGCTACT
    GAGACAGACA GAAAAGACCT GGTCCTGTAC TGTGAAGCCT TCCTGACCAC CTACAGAACC
    TTCATCAGCC CCGAGGAGCT CATCAAGAAG CTGCAGTGCC GGTACGAGAA GTTCTCTCCC
    TTTGCTGACA CATTCAAGAA GCGAGTGAGC AAGAACACAT TCTTCGTGCT GGTGCGGGTG
    GTGGACGAGC TCTGCCTGGT GGAGCTGACA GAGGAGATCC TGAAGCTGCT GATGGAGCTA
    GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC AGCTTGGCCA GGGTACTCCG AAAGAACATT
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCC ATTCCGACCA GCCTTTGGCA
    GCCAGGGGTG TTGCAGCCAG GCCAGGAACC TTGCATGATT TCCACAGCCA CGAGATAGCT
    GAACAGCTGA CACTGCTGGA TGCCGAGCTT TTCTATAAGA TAGAGATTCC CGAAGTTTTG
    CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG AGCCCCAATC TGACCCAGTT CACAGAGCAC
    TTCAACAACA TGTCCTACTG GGTGCGGTCC GTCATCATGC TGCAGGAGAA GGCCCAGGAC
    CGGGAGAGGC TGCTCCTGAA GTTCATCAAG ATCATGAAGC ACCTGCGCAA GCTCAACAAT
    TTCAACTCCT ACCTGGCCAT CCTCTCCGCA CTGGACTCGG CCCCCATCCG CAGACTGGAG
    TGGCAGCGAC AGACCTCAGA GGGCCTGGCT GAGTACTGCA CGTTGATTGA CAGCTCATCC
    TCCTTCCGAG CCTACCGGGC TGCCCTCTCA GAGGTGGAAC CTCCATGTAT CCCATACCTA
    GGTTTGATCC TGCAAGACCT GACGTTCGTC CACCTGGGAA ACCCAGACTA CATAGACGGG
    AAGGTCAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TGGACAGCAT GCGCTGTTTC
    CAGCAGGTGC ACTATGAAAT CCGGAGAAAC GACGACATCA TAAACTTCTT CAATGACTTC
    AGCGACCACC TGGCCGAGGA AGCCCTATGG GAACTCTCTC TGAAGATCAA GCCTAGGAAC
    ATAACAAGGC GGAAAACAGA CCGTGAAGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773536.1
    CDS16..3354
    Translation

    Target ORF information:

    RefSeq Version XM_008775314.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775314.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTCCACC AGCATTACCA CCCAAGAAAA GGCAATCTGC TCCATCCCCT
    ACCCGGGTGG CTGTGGTAGC CCCCATGAGT CGTGCTGTCA GTGGCTCCAG TTTGCCTGTT
    GGAATCAATA GGCAGGACTT TGATGTTGAA TGTTACACCC AGAGGCGCCT GTCAGGAGGC
    AGCCGCTCCT GTGGTGGTGA ATCTCCTCGC CTGTCCCCCT GCAGCAGTAC AGGCAAGCTC
    AGCCGGTCAG ACGAGCAGCT GTCCTCCCTA GACAGGGATA GCGGGCAGTG CTCACGGAAC
    ACAAGCTGTG AAACACTAGA TCACTATGAC CCCGACTATG AATTCCTCCA GCAAGACCTC
    TCCAATGCAG ACCAGACCCC TCCACAGGCA GCCTGTAACC TCAGCCCACT GCCGGAGTCC
    ATGGGGGAGT CTGGGCCCCC ATTTGTTGGC TACTCTTTCC AGCTGCCTCT GGGCAGCTGT
    CTCCAGCAGG ATGGACAGCA GACAGATACT CCACCTGCCC TTCCTGAGAA GAAGCGTAGG
    AGCGCAGTCT CCCAGACCAC GGACAGCTCT GGCTGCAGGG TGTCCTATGA GCGACACCCC
    TCACAGTATG ACAACATCTC AGAAGATGAT CTGCAGAACC CAGCCCAGCC TGTGTCCTAC
    CCGCCCTTTG CTGCTATCCT GCCCTTTCAG CAGGGAGCTT CTTCCACCCC TGTTGAGTTT
    GTGGGTGATT TCAGTGTTCC TGAGTCAGCC GGTGACCCAG AAAAGCCACC TCCTCTACCA
    GAGAAGAAAA ACAAACACAT GCTGGCCTAC ATGCAACTGC TGGAGGACTA CTCAGAGCCA
    CAGCCCTCCA TGTTCTACCA GACACCGCAG AGTGAGCACA TCTACCAGCA GAAGAACAAG
    ATGCTCATGG AGGTCTATGG CTTCAGCGAC TCCTTCTGTG GTAGTGACTC CACGCAGGAG
    CTGGCCCCTC CACCCGCTTT GCCCCCCAAG CAGCGGCAGC TGGAACCGCC ATCTGGGAAG
    GACGGACATC CCAGAGATCT TTCGGTCAGC AGTGCATCTG GGAAGGACAG CAGAGAAAAT
    GGGGAGAGGT CCCCGAAGTC ACTGGATGCT CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG
    GATGAACTGT CCCTCATTGA CCACAATGAA ATCATGGCCA GGCTGACACT CAAGCAAGAG
    GGTGATGATG GACCAGATGT TCGTGGAGGT TCAGGAGACA TCTTACTGGT CCATGCTACT
    GAGACAGACA GAAAAGACCT GGTCCTGTAC TGTGAAGCCT TCCTGACCAC CTACAGAACC
    TTCATCAGCC CCGAGGAGCT CATCAAGAAG CTGCAGTGCC GGTACGAGAA GTTCTCTCCC
    TTTGCTGACA CATTCAAGAA GCGAGTGAGC AAGAACACAT TCTTCGTGCT GGTGCGGGTG
    GTGGACGAGC TCTGCCTGGT GGAGCTGACA GAGGAGATCC TGAAGCTGCT GATGGAGCTA
    GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC AGCTTGGCCA GGGTACTCCG AAAGAACATT
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCC ATTCCGACCA GCCTTTGGCA
    GCCAGGGGTG TTGCAGCCAG GCCAGGAACC TTGCATGATT TCCACAGCCA CGAGATAGCT
    GAACAGCTGA CACTGCTGGA TGCCGAGCTT TTCTATAAGA TAGAGATTCC CGAAGTTTTG
    CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG AGCCCCAATC TGACCCAGTT CACAGAGCAC
    TTCAACAACA TGTCCTACTG GGTGCGGTCC GTCATCATGC TGCAGGAGAA GGCCCAGGAC
    CGGGAGAGGC TGCTCCTGAA GTTCATCAAG ATCATGAAGC ACCTGCGCAA GCTCAACAAT
    TTCAACTCCT ACCTGGCCAT CCTCTCCGCA CTGGACTCGG CCCCCATCCG CAGACTGGAG
    TGGCAGCGAC AGACCTCAGA GGGCCTGGCT GAGTACTGCA CGTTGATTGA CAGCTCATCC
    TCCTTCCGAG CCTACCGGGC TGCCCTCTCA GAGGTGGAAC CTCCATGTAT CCCATACCTA
    GGTTTGATCC TGCAAGACCT GACGTTCGTC CACCTGGGAA ACCCAGACTA CATAGACGGG
    AAGGTCAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TGGACAGCAT GCGCTGTTTC
    CAGCAGGTGC ACTATGAAAT CCGGAGAAAC GACGACATCA TAAACTTCTT CAATGACTTC
    AGCGACCACC TGGCCGAGGA AGCCCTATGG GAACTCTCTC TGAAGATCAA GCCTAGGAAC
    ATAACAAGGC GGAAAACAGA CCGTGAAGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55077
    Clone ID Related Accession (Same CDS sequence) XM_017601886.1
    Accession Version XM_017601886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGAGTGAAA ACGCCAGCAG TGAGGAAGCT GGTGGGGGTG AATATGTCAA TCTGTACTCC
    TCTGGCCAGA CCAGCGAGGA GCTAGCTCCC TCTGGAGGAG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457375.1
    CDS7..3882
    Translation

    Target ORF information:

    RefSeq Version XM_017601886.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGAGTGAAA ACGCCAGCAG TGAGGAAGCT GGTGGGGGTG AATATGTCAA TCTGTACTCC
    TCTGGCCAGA CCAGCGAGGA GCTAGCTCCC TCTGGAGGAG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55078
    Clone ID Related Accession (Same CDS sequence) XM_017601887.1
    Accession Version XM_017601887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGG CCTCCTATGC TGCTTCTTCC TTCTCTGTCT CCCACTGTGT CCAGCAAACT
    AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCCTCAGTGT ATCCGTGTCT
    AACTCCTTTC TCAACCGGCA CGGCAGCTTG CCTGTGCCCT CGTACAAATC TGTGTTTAGG
    TCTTACTCCC AGGACTTCAT GCCTCACCAC CAAGCCTCCG TCCAACCTTT CCTTCCGCCT
    ACCTCCTCTT CCTCTCCACA TTTCCCACCT GTCCACGCGT CCCAGAGCTC GGACTTAGCG
    GTGCCCACCG TAAGCAGTCC GCCTCCCAGC ACCGTGGACG GGCCTCTCTC GTCTTCTCAG
    GACAGCAGCT TTCATGGGAA CCCTGTCCGC CTTCCTTCCG AAACCTCTTT CACTGACTCG
    AGTGAAAACG CCAGCAGTGA GGAAGCTGGT GGGGGTGAAT ATGTCAATCT GTACTCCTCT
    GGCCAGACCA GCGAGGAGCT AGCTCCCTCT GGAGGAGAAC CGCCATCTGG GAAGGACGGA
    CATCCCAGAG ATCTTTCGGT CAGCAGTGCA TCTGGGAAGG ACAGCAGAGA AAATGGGGAG
    AGGTCCCCGA AGTCACTGGA TGCTCTGGAG TCAGCCCAGT CAGAAGAGGA AGTGGATGAA
    CTGTCCCTCA TTGACCACAA TGAAATCATG GCCAGGCTGA CACTCAAGCA AGAGGGTGAT
    GATGGACCAG ATGTTCGTGG AGGTTCAGGA GACATCTTAC TGGTCCATGC TACTGAGACA
    GACAGAAAAG ACCTGGTCCT GTACTGTGAA GCCTTCCTGA CCACCTACAG AACCTTCATC
    AGCCCCGAGG AGCTCATCAA GAAGCTGCAG TGCCGGTACG AGAAGTTCTC TCCCTTTGCT
    GACACATTCA AGAAGCGAGT GAGCAAGAAC ACATTCTTCG TGCTGGTGCG GGTGGTGGAC
    GAGCTCTGCC TGGTGGAGCT GACAGAGGAG ATCCTGAAGC TGCTGATGGA GCTAGTCTTC
    CGCCTAGTGT GCAGTGGAGA GCTCAGCTTG GCCAGGGTAC TCCGAAAGAA CATTCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCCATTCCG ACCAGCCTTT GGCAGCCAGG
    GGTGTTGCAG CCAGGCCAGG AACCTTGCAT GATTTCCACA GCCACGAGAT AGCTGAACAG
    CTGACACTGC TGGATGCCGA GCTTTTCTAT AAGATAGAGA TTCCCGAAGT TTTGCTTTGG
    GCCAAAGAGC AGAATGAAGA GAAGAGCCCC AATCTGACCC AGTTCACAGA GCACTTCAAC
    AACATGTCCT ACTGGGTGCG GTCCGTCATC ATGCTGCAGG AGAAGGCCCA GGACCGGGAG
    AGGCTGCTCC TGAAGTTCAT CAAGATCATG AAGCACCTGC GCAAGCTCAA CAATTTCAAC
    TCCTACCTGG CCATCCTCTC CGCACTGGAC TCGGCCCCCA TCCGCAGACT GGAGTGGCAG
    CGACAGACCT CAGAGGGCCT GGCTGAGTAC TGCACGTTGA TTGACAGCTC ATCCTCCTTC
    CGAGCCTACC GGGCTGCCCT CTCAGAGGTG GAACCTCCAT GTATCCCATA CCTAGGTTTG
    ATCCTGCAAG ACCTGACGTT CGTCCACCTG GGAAACCCAG ACTACATAGA CGGGAAGGTC
    AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGCTG TTTCCAGCAG
    GTGCACTATG AAATCCGGAG AAACGACGAC ATCATAAACT TCTTCAATGA CTTCAGCGAC
    CACCTGGCCG AGGAAGCCCT ATGGGAACTC TCTCTGAAGA TCAAGCCTAG GAACATAACA
    AGGCGGAAAA CAGACCGTGA AGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457376.1
    CDS7..3879
    Translation

    Target ORF information:

    RefSeq Version XM_017601887.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGG CCTCCTATGC TGCTTCTTCC TTCTCTGTCT CCCACTGTGT CCAGCAAACT
    AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCCTCAGTGT ATCCGTGTCT
    AACTCCTTTC TCAACCGGCA CGGCAGCTTG CCTGTGCCCT CGTACAAATC TGTGTTTAGG
    TCTTACTCCC AGGACTTCAT GCCTCACCAC CAAGCCTCCG TCCAACCTTT CCTTCCGCCT
    ACCTCCTCTT CCTCTCCACA TTTCCCACCT GTCCACGCGT CCCAGAGCTC GGACTTAGCG
    GTGCCCACCG TAAGCAGTCC GCCTCCCAGC ACCGTGGACG GGCCTCTCTC GTCTTCTCAG
    GACAGCAGCT TTCATGGGAA CCCTGTCCGC CTTCCTTCCG AAACCTCTTT CACTGACTCG
    AGTGAAAACG CCAGCAGTGA GGAAGCTGGT GGGGGTGAAT ATGTCAATCT GTACTCCTCT
    GGCCAGACCA GCGAGGAGCT AGCTCCCTCT GGAGGAGAAC CGCCATCTGG GAAGGACGGA
    CATCCCAGAG ATCTTTCGGT CAGCAGTGCA TCTGGGAAGG ACAGCAGAGA AAATGGGGAG
    AGGTCCCCGA AGTCACTGGA TGCTCTGGAG TCAGCCCAGT CAGAAGAGGA AGTGGATGAA
    CTGTCCCTCA TTGACCACAA TGAAATCATG GCCAGGCTGA CACTCAAGCA AGAGGGTGAT
    GATGGACCAG ATGTTCGTGG AGGTTCAGGA GACATCTTAC TGGTCCATGC TACTGAGACA
    GACAGAAAAG ACCTGGTCCT GTACTGTGAA GCCTTCCTGA CCACCTACAG AACCTTCATC
    AGCCCCGAGG AGCTCATCAA GAAGCTGCAG TGCCGGTACG AGAAGTTCTC TCCCTTTGCT
    GACACATTCA AGAAGCGAGT GAGCAAGAAC ACATTCTTCG TGCTGGTGCG GGTGGTGGAC
    GAGCTCTGCC TGGTGGAGCT GACAGAGGAG ATCCTGAAGC TGCTGATGGA GCTAGTCTTC
    CGCCTAGTGT GCAGTGGAGA GCTCAGCTTG GCCAGGGTAC TCCGAAAGAA CATTCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCCATTCCG ACCAGCCTTT GGCAGCCAGG
    GGTGTTGCAG CCAGGCCAGG AACCTTGCAT GATTTCCACA GCCACGAGAT AGCTGAACAG
    CTGACACTGC TGGATGCCGA GCTTTTCTAT AAGATAGAGA TTCCCGAAGT TTTGCTTTGG
    GCCAAAGAGC AGAATGAAGA GAAGAGCCCC AATCTGACCC AGTTCACAGA GCACTTCAAC
    AACATGTCCT ACTGGGTGCG GTCCGTCATC ATGCTGCAGG AGAAGGCCCA GGACCGGGAG
    AGGCTGCTCC TGAAGTTCAT CAAGATCATG AAGCACCTGC GCAAGCTCAA CAATTTCAAC
    TCCTACCTGG CCATCCTCTC CGCACTGGAC TCGGCCCCCA TCCGCAGACT GGAGTGGCAG
    CGACAGACCT CAGAGGGCCT GGCTGAGTAC TGCACGTTGA TTGACAGCTC ATCCTCCTTC
    CGAGCCTACC GGGCTGCCCT CTCAGAGGTG GAACCTCCAT GTATCCCATA CCTAGGTTTG
    ATCCTGCAAG ACCTGACGTT CGTCCACCTG GGAAACCCAG ACTACATAGA CGGGAAGGTC
    AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTGGACA GCATGCGCTG TTTCCAGCAG
    GTGCACTATG AAATCCGGAG AAACGACGAC ATCATAAACT TCTTCAATGA CTTCAGCGAC
    CACCTGGCCG AGGAAGCCCT ATGGGAACTC TCTCTGAAGA TCAAGCCTAG GAACATAACA
    AGGCGGAAAA CAGACCGTGA AGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55079
    Clone ID Related Accession (Same CDS sequence) XM_017601888.1 , XM_017592102.1
    Accession Version XM_017601888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGA GTGAAAACGC CAGCAGTGAG
    GAAGCTGGTG GGGGTGAATA TGTCAATCTG TACTCCTCTG GCCAGACCAG CGAGGAGCTA
    GCTCCCTCTG GAGGAGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457377.1
    CDS3181..6972
    Translation

    Target ORF information:

    RefSeq Version XM_017601888.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGA GTGAAAACGC CAGCAGTGAG
    GAAGCTGGTG GGGGTGAATA TGTCAATCTG TACTCCTCTG GCCAGACCAG CGAGGAGCTA
    GCTCCCTCTG GAGGAGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55079
    Clone ID Related Accession (Same CDS sequence) XM_017601888.1 , XM_017592102.1
    Accession Version XM_017592102.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGA GTGAAAACGC CAGCAGTGAG
    GAAGCTGGTG GGGGTGAATA TGTCAATCTG TACTCCTCTG GCCAGACCAG CGAGGAGCTA
    GCTCCCTCTG GAGGAGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447591.1
    CDS3181..6972
    Translation

    Target ORF information:

    RefSeq Version XM_017592102.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592102.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGA GTGAAAACGC CAGCAGTGAG
    GAAGCTGGTG GGGGTGAATA TGTCAATCTG TACTCCTCTG GCCAGACCAG CGAGGAGCTA
    GCTCCCTCTG GAGGAGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55080
    Clone ID Related Accession (Same CDS sequence) XM_017601889.1
    Accession Version XM_017601889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGGAACCGC CATCTGGGAA GGACGGACAT CCCAGAGATC TTTCGGTCAG CAGTGCATCT
    GGGAAGGACA GCAGAGAAAA TGGGGAGAGG TCCCCGAAGT CACTGGATGC TCTGGAGTCA
    GCCCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATCATGGCC
    AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGACCAGATG TTCGTGGAGG TTCAGGAGAC
    ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTCCTGTA CTGTGAAGCC
    TTCCTGACCA CCTACAGAAC CTTCATCAGC CCCGAGGAGC TCATCAAGAA GCTGCAGTGC
    CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACATTCAAGA AGCGAGTGAG CAAGAACACA
    TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC
    CTGAAGCTGC TGATGGAGCT AGTCTTCCGC CTAGTGTGCA GTGGAGAGCT CAGCTTGGCC
    AGGGTACTCC GAAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    CATTCCGACC AGCCTTTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT
    TTCCACAGCC ACGAGATAGC TGAACAGCTG ACACTGCTGG ATGCCGAGCT TTTCTATAAG
    ATAGAGATTC CCGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAAGAGAA GAGCCCCAAT
    CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CGTCATCATG
    CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTGA AGTTCATCAA GATCATGAAG
    CACCTGCGCA AGCTCAACAA TTTCAACTCC TACCTGGCCA TCCTCTCCGC ACTGGACTCG
    GCCCCCATCC GCAGACTGGA GTGGCAGCGA CAGACCTCAG AGGGCCTGGC TGAGTACTGC
    ACGTTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCCCTCTC AGAGGTGGAA
    CCTCCATGTA TCCCATACCT AGGTTTGATC CTGCAAGACC TGACGTTCGT CCACCTGGGA
    AACCCAGACT ACATAGACGG GAAGGTCAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC
    CTGGACAGCA TGCGCTGTTT CCAGCAGGTG CACTATGAAA TCCGGAGAAA CGACGACATC
    ATAAACTTCT TCAATGACTT CAGCGACCAC CTGGCCGAGG AAGCCCTATG GGAACTCTCT
    CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGTGAAGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457378.1
    CDS6..3785
    Translation

    Target ORF information:

    RefSeq Version XM_017601889.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGGAACCGC CATCTGGGAA GGACGGACAT CCCAGAGATC TTTCGGTCAG CAGTGCATCT
    GGGAAGGACA GCAGAGAAAA TGGGGAGAGG TCCCCGAAGT CACTGGATGC TCTGGAGTCA
    GCCCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATCATGGCC
    AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGACCAGATG TTCGTGGAGG TTCAGGAGAC
    ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTCCTGTA CTGTGAAGCC
    TTCCTGACCA CCTACAGAAC CTTCATCAGC CCCGAGGAGC TCATCAAGAA GCTGCAGTGC
    CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACATTCAAGA AGCGAGTGAG CAAGAACACA
    TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC
    CTGAAGCTGC TGATGGAGCT AGTCTTCCGC CTAGTGTGCA GTGGAGAGCT CAGCTTGGCC
    AGGGTACTCC GAAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    CATTCCGACC AGCCTTTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT
    TTCCACAGCC ACGAGATAGC TGAACAGCTG ACACTGCTGG ATGCCGAGCT TTTCTATAAG
    ATAGAGATTC CCGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAAGAGAA GAGCCCCAAT
    CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CGTCATCATG
    CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTGA AGTTCATCAA GATCATGAAG
    CACCTGCGCA AGCTCAACAA TTTCAACTCC TACCTGGCCA TCCTCTCCGC ACTGGACTCG
    GCCCCCATCC GCAGACTGGA GTGGCAGCGA CAGACCTCAG AGGGCCTGGC TGAGTACTGC
    ACGTTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCCCTCTC AGAGGTGGAA
    CCTCCATGTA TCCCATACCT AGGTTTGATC CTGCAAGACC TGACGTTCGT CCACCTGGGA
    AACCCAGACT ACATAGACGG GAAGGTCAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC
    CTGGACAGCA TGCGCTGTTT CCAGCAGGTG CACTATGAAA TCCGGAGAAA CGACGACATC
    ATAAACTTCT TCAATGACTT CAGCGACCAC CTGGCCGAGG AAGCCCTATG GGAACTCTCT
    CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGTGAAGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55081
    Clone ID Related Accession (Same CDS sequence) XM_017601890.1
    Accession Version XM_017601890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TTTGCCTGGA TTTTCTGTCT CTTCTAGTCC ACCAGCATTA
    CCACCCAAGA AAAGGCAATC TGCTCCATCC CCTACCCGGG TGGCTGTGGT AGCCCCCATG
    AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA CTTTGATGTT
    GAATGTTACA CCCAGAGGCG CCTGTCAGGA GGCAGCCGCT CCTGTGGTGG TGAATCTCCT
    CGCCTGTCCC CCTGCAGCAG TACAGGCAAG CTCAGCCGGT CAGACGAGCA GCTGTCCTCC
    CTAGACAGGG ATAGCGGGCA GTGCTCACGG AACACAAGCT GTGAAACACT AGATCACTAT
    GACCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCCAATG CAGACCAGAC CCCTCCACAG
    GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG TCCATGGGGG AGTCTGGGCC CCCATTTGTT
    GGCTACTCTT TCCAGCTGCC TCTGGGCAGC TGTCTCCAGC AGGATGGACA GCAGACAGAT
    ACTCCACCTG CCCTTCCTGA GAAGAAGCGT AGGAGCGCAG TCTCCCAGAC CACGGACAGC
    TCTGGCTGCA GGGTGTCCTA TGAGCGACAC CCCTCACAGT ATGACAACAT CTCAGAAGAT
    GATCTGCAGA ACCCAGCCCA GCCTGTGTCC TACCCGCCCT TTGCTGCTAT CCTGCCCTTT
    CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA
    GCCGGTGACC CAGAAAAGCC ACCTCCTCTA CCAGAGAAGA AAAACAAACA CATGCTGGCC
    TACATGCAAC TGCTGGAGGA CTACTCAGAG CCACAGCCCT CCATGTTCTA CCAGACACCG
    CAGAGTGAGC ACATCTACCA GCAGAAGAAC AAGATGCTCA TGGAGGTCTA TGGCTTCAGC
    GACTCCTTCT GTGGTAGTGA CTCCACGCAG GAGCTGGCCC CTCCACCCGC TTTGCCCCCC
    AAGCAGCGGC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCTGTCTC CCACTGTGTC
    CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTA
    TCCGTGTCTA ACTCCTTTCT CAACCGGCAC GGCAGCTTGC CTGTGCCCTC GTACAAATCT
    GTGTTTAGGT CTTACTCCCA GGACTTCATG CCTCACCACC AAGCCTCCGT CCAACCTTTC
    CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG TCCACGCGTC CCAGAGCTCG
    GACTTAGCGG TGCCCACCGT AAGCAGTCCG CCTCCCAGCA CCGTGGACGG GCCTCTCTCG
    TCTTCTCAGG ACAGCAGCTT TCATGGGAAC CCTGTCCGCC TTCCTTCCGA AACCTCTTTC
    ACTGACTCGA GTGAAAACGC CAGCAGTGAG GAAGCTGGTG GGGGTGAATA TGTCAATCTG
    TACTCCTCTG GCCAGACCAG CGAGGAGCTA GCTCCCTCTG GAGGAGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457379.1
    CDS7..3768
    Translation

    Target ORF information:

    RefSeq Version XM_017601890.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAAACCT GTGCTGGCTG
    GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA
    ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG
    GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCT
    TGCTATAGCC GAGTGTACCA GAGCCTTGCC AACCTTATCC GCTGGTCTGA CCAGGTGATG
    CTCGAGGGTG TGAACTCAGA AGATAAGGAG ATGGTGACGA CTGTGAAGGG TGTCATCAAA
    GCTGTGCTGG ATGGAGTGAA GGAACTGGTG AGGCTAACCA TTGAGAAGCA AGGCCGGCCA
    TCACCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG
    CTCCCTCTGA CAGAACGAGA AATGGAGATT CTGAACAAGA CAAGTGTGTC ACCATCCACT
    GAACTGCTCC CAGACTCCAC CAGTGAAGAG GTTGCGCCCC CCAAGCCCCC TTTACCTGGC
    ATCCGGGTGG TTGATAACAG TTTGCCTGGA TTTTCTGTCT CTTCTAGTCC ACCAGCATTA
    CCACCCAAGA AAAGGCAATC TGCTCCATCC CCTACCCGGG TGGCTGTGGT AGCCCCCATG
    AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA CTTTGATGTT
    GAATGTTACA CCCAGAGGCG CCTGTCAGGA GGCAGCCGCT CCTGTGGTGG TGAATCTCCT
    CGCCTGTCCC CCTGCAGCAG TACAGGCAAG CTCAGCCGGT CAGACGAGCA GCTGTCCTCC
    CTAGACAGGG ATAGCGGGCA GTGCTCACGG AACACAAGCT GTGAAACACT AGATCACTAT
    GACCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCCAATG CAGACCAGAC CCCTCCACAG
    GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG TCCATGGGGG AGTCTGGGCC CCCATTTGTT
    GGCTACTCTT TCCAGCTGCC TCTGGGCAGC TGTCTCCAGC AGGATGGACA GCAGACAGAT
    ACTCCACCTG CCCTTCCTGA GAAGAAGCGT AGGAGCGCAG TCTCCCAGAC CACGGACAGC
    TCTGGCTGCA GGGTGTCCTA TGAGCGACAC CCCTCACAGT ATGACAACAT CTCAGAAGAT
    GATCTGCAGA ACCCAGCCCA GCCTGTGTCC TACCCGCCCT TTGCTGCTAT CCTGCCCTTT
    CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA
    GCCGGTGACC CAGAAAAGCC ACCTCCTCTA CCAGAGAAGA AAAACAAACA CATGCTGGCC
    TACATGCAAC TGCTGGAGGA CTACTCAGAG CCACAGCCCT CCATGTTCTA CCAGACACCG
    CAGAGTGAGC ACATCTACCA GCAGAAGAAC AAGATGCTCA TGGAGGTCTA TGGCTTCAGC
    GACTCCTTCT GTGGTAGTGA CTCCACGCAG GAGCTGGCCC CTCCACCCGC TTTGCCCCCC
    AAGCAGCGGC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCTGTCTC CCACTGTGTC
    CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTA
    TCCGTGTCTA ACTCCTTTCT CAACCGGCAC GGCAGCTTGC CTGTGCCCTC GTACAAATCT
    GTGTTTAGGT CTTACTCCCA GGACTTCATG CCTCACCACC AAGCCTCCGT CCAACCTTTC
    CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG TCCACGCGTC CCAGAGCTCG
    GACTTAGCGG TGCCCACCGT AAGCAGTCCG CCTCCCAGCA CCGTGGACGG GCCTCTCTCG
    TCTTCTCAGG ACAGCAGCTT TCATGGGAAC CCTGTCCGCC TTCCTTCCGA AACCTCTTTC
    ACTGACTCGA GTGAAAACGC CAGCAGTGAG GAAGCTGGTG GGGGTGAATA TGTCAATCTG
    TACTCCTCTG GCCAGACCAG CGAGGAGCTA GCTCCCTCTG GAGGAGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55082
    Clone ID Related Accession (Same CDS sequence) XM_017601891.1
    Accession Version XM_017601891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA
    GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC
    CACGCGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC
    GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT
    CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAACGCCA GCAGTGAGGA AGCTGGTGGG
    GGTGAATATG TCAATCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTAGC TCCCTCTGGA
    GGAGAACCGC CATCTGGGAA GGACGGACAT CCCAGAGATC TTTCGGTCAG CAGTGCATCT
    GGGAAGGACA GCAGAGAAAA TGGGGAGAGG TCCCCGAAGT CACTGGATGC TCTGGAGTCA
    GCCCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATCATGGCC
    AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGACCAGATG TTCGTGGAGG TTCAGGAGAC
    ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTCCTGTA CTGTGAAGCC
    TTCCTGACCA CCTACAGAAC CTTCATCAGC CCCGAGGAGC TCATCAAGAA GCTGCAGTGC
    CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACATTCAAGA AGCGAGTGAG CAAGAACACA
    TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC
    CTGAAGCTGC TGATGGAGCT AGTCTTCCGC CTAGTGTGCA GTGGAGAGCT CAGCTTGGCC
    AGGGTACTCC GAAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    CATTCCGACC AGCCTTTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT
    TTCCACAGCC ACGAGATAGC TGAACAGCTG ACACTGCTGG ATGCCGAGCT TTTCTATAAG
    ATAGAGATTC CCGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAAGAGAA GAGCCCCAAT
    CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CGTCATCATG
    CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTGA AGTTCATCAA GATCATGAAG
    CACCTGCGCA AGCTCAACAA TTTCAACTCC TACCTGGCCA TCCTCTCCGC ACTGGACTCG
    GCCCCCATCC GCAGACTGGA GTGGCAGCGA CAGACCTCAG AGGGCCTGGC TGAGTACTGC
    ACGTTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCCCTCTC AGAGGTGGAA
    CCTCCATGTA TCCCATACCT AGGTTTGATC CTGCAAGACC TGACGTTCGT CCACCTGGGA
    AACCCAGACT ACATAGACGG GAAGGTCAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC
    CTGGACAGCA TGCGCTGTTT CCAGCAGGTG CACTATGAAA TCCGGAGAAA CGACGACATC
    ATAAACTTCT TCAATGACTT CAGCGACCAC CTGGCCGAGG AAGCCCTATG GGAACTCTCT
    CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGTGAAGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457380.1
    CDS6..3725
    Translation

    Target ORF information:

    RefSeq Version XM_017601891.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA
    GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC
    CACGCGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC
    GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT
    CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAACGCCA GCAGTGAGGA AGCTGGTGGG
    GGTGAATATG TCAATCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTAGC TCCCTCTGGA
    GGAGAACCGC CATCTGGGAA GGACGGACAT CCCAGAGATC TTTCGGTCAG CAGTGCATCT
    GGGAAGGACA GCAGAGAAAA TGGGGAGAGG TCCCCGAAGT CACTGGATGC TCTGGAGTCA
    GCCCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATCATGGCC
    AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGACCAGATG TTCGTGGAGG TTCAGGAGAC
    ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTCCTGTA CTGTGAAGCC
    TTCCTGACCA CCTACAGAAC CTTCATCAGC CCCGAGGAGC TCATCAAGAA GCTGCAGTGC
    CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACATTCAAGA AGCGAGTGAG CAAGAACACA
    TTCTTCGTGC TGGTGCGGGT GGTGGACGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC
    CTGAAGCTGC TGATGGAGCT AGTCTTCCGC CTAGTGTGCA GTGGAGAGCT CAGCTTGGCC
    AGGGTACTCC GAAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    CATTCCGACC AGCCTTTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT
    TTCCACAGCC ACGAGATAGC TGAACAGCTG ACACTGCTGG ATGCCGAGCT TTTCTATAAG
    ATAGAGATTC CCGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAAGAGAA GAGCCCCAAT
    CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CGTCATCATG
    CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTGA AGTTCATCAA GATCATGAAG
    CACCTGCGCA AGCTCAACAA TTTCAACTCC TACCTGGCCA TCCTCTCCGC ACTGGACTCG
    GCCCCCATCC GCAGACTGGA GTGGCAGCGA CAGACCTCAG AGGGCCTGGC TGAGTACTGC
    ACGTTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCCCTCTC AGAGGTGGAA
    CCTCCATGTA TCCCATACCT AGGTTTGATC CTGCAAGACC TGACGTTCGT CCACCTGGGA
    AACCCAGACT ACATAGACGG GAAGGTCAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC
    CTGGACAGCA TGCGCTGTTT CCAGCAGGTG CACTATGAAA TCCGGAGAAA CGACGACATC
    ATAAACTTCT TCAATGACTT CAGCGACCAC CTGGCCGAGG AAGCCCTATG GGAACTCTCT
    CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGTGAAGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55083
    Clone ID Related Accession (Same CDS sequence) XM_017601892.1
    Accession Version XM_017601892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457381.1
    CDS5..3526
    Translation

    Target ORF information:

    RefSeq Version XM_017601892.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGC AGGCCTCCTA TGCTGCTTCT TCCTTCTCTG TCTCCCACTG TGTCCAGCAA
    ACTAAAGTGG CCTTCACCCC CGAGGACGGC AGTGCCGCTC AGGGCCTCAG TGTATCCGTG
    TCTAACTCCT TTCTCAACCG GCACGGCAGC TTGCCTGTGC CCTCGGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa55084
    Clone ID Related Accession (Same CDS sequence) XM_017601893.1
    Accession Version XM_017601893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGG AACCGCCATC TGGGAAGGAC GGACATCCCA GAGATCTTTC GGTCAGCAGT
    GCATCTGGGA AGGACAGCAG AGAAAATGGG GAGAGGTCCC CGAAGTCACT GGATGCTCTG
    GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT GAACTGTCCC TCATTGACCA CAATGAAATC
    ATGGCCAGGC TGACACTCAA GCAAGAGGGT GATGATGGAC CAGATGTTCG TGGAGGTTCA
    GGAGACATCT TACTGGTCCA TGCTACTGAG ACAGACAGAA AAGACCTGGT CCTGTACTGT
    GAAGCCTTCC TGACCACCTA CAGAACCTTC ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG
    CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT GCTGACACAT TCAAGAAGCG AGTGAGCAAG
    AACACATTCT TCGTGCTGGT GCGGGTGGTG GACGAGCTCT GCCTGGTGGA GCTGACAGAG
    GAGATCCTGA AGCTGCTGAT GGAGCTAGTC TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC
    TTGGCCAGGG TACTCCGAAA GAACATTCTG GACAAGGTGG ACCAGAAGAA GCTGCTCAGG
    TGTGCCCATT CCGACCAGCC TTTGGCAGCC AGGGGTGTTG CAGCCAGGCC AGGAACCTTG
    CATGATTTCC ACAGCCACGA GATAGCTGAA CAGCTGACAC TGCTGGATGC CGAGCTTTTC
    TATAAGATAG AGATTCCCGA AGTTTTGCTT TGGGCCAAAG AGCAGAATGA AGAGAAGAGC
    CCCAATCTGA CCCAGTTCAC AGAGCACTTC AACAACATGT CCTACTGGGT GCGGTCCGTC
    ATCATGCTGC AGGAGAAGGC CCAGGACCGG GAGAGGCTGC TCCTGAAGTT CATCAAGATC
    ATGAAGCACC TGCGCAAGCT CAACAATTTC AACTCCTACC TGGCCATCCT CTCCGCACTG
    GACTCGGCCC CCATCCGCAG ACTGGAGTGG CAGCGACAGA CCTCAGAGGG CCTGGCTGAG
    TACTGCACGT TGATTGACAG CTCATCCTCC TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG
    GTGGAACCTC CATGTATCCC ATACCTAGGT TTGATCCTGC AAGACCTGAC GTTCGTCCAC
    CTGGGAAACC CAGACTACAT AGACGGGAAG GTCAACTTCT CCAAGCGGTG GCAGCAGTTC
    AACATCCTGG ACAGCATGCG CTGTTTCCAG CAGGTGCACT ATGAAATCCG GAGAAACGAC
    GACATCATAA ACTTCTTCAA TGACTTCAGC GACCACCTGG CCGAGGAAGC CCTATGGGAA
    CTCTCTCTGA AGATCAAGCC TAGGAACATA ACAAGGCGGA AAACAGACCG TGAAGAGAAG
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457382.1
    CDS5..3370
    Translation

    Target ORF information:

    RefSeq Version XM_017601893.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCAAA AAGAAAAAAG ACCTGGTCCA CATGTCCCTT CCTTTGACTC TTTCAAGAAG 
    GAAGTTTTAG CTAAAAGACC ACATCACTCC CACAAGAACC ACAGCCTCTC CTGCCCCTCC
    TCTAACAGTC AGACTTCTGT GCTTTCAGAC TCTCAGCGCT CTCATCTCTC CTCCTTCACC
    ATGAAGCTGA TGGACAAATT CCACTCTCCT AAAATCAAGA GAACACCATC CAAGAAGGGA
    AAGCCAGCTG AGGTGTCGAA GACTCCTGAG AAGCCTGTGA GCAAAGAGGC AAGAGACAGA
    TTTCTACCAG AGGGCTACCC TATCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG
    TCCACCAGTG CTGTGGATTC CAGGTCTCAG AGGCAGAAGA ACCTGTGCTG GCTGGAGGAG
    AAAGAGAAGG AGGTTGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCC
    ATTGATAAGA AGGTTCTGGA GATGCTCCCG GGGTCAGCCA GCAAGGTGCT GGAGGCCATC
    TTACCCCTGG TGCAGACTGA CCCTCGGATC CAGCACAGCT CAGCCCTCTC CTCTTGCTAT
    AGCCGAGTGT ACCAGAGCCT TGCCAACCTT ATCCGCTGGT CTGACCAGGT GATGCTCGAG
    GGTGTGAACT CAGAAGATAA GGAGATGGTG ACGACTGTGA AGGGTGTCAT CAAAGCTGTG
    CTGGATGGAG TGAAGGAACT GGTGAGGCTA ACCATTGAGA AGCAAGGCCG GCCATCACCA
    ACAAGCCCAG TGAAGCCCAG TTCCCCAGCC AGCAAGCCTG ATGGCCAGCC TGAGCTCCCT
    CTGACAGAAC GAGAAATGGA GATTCTGAAC AAGACAAGTG TGTCACCATC CACTGAACTG
    CTCCCAGACT CCACCAGTGA AGAGGTTGCG CCCCCCAAGC CCCCTTTACC TGGCATCCGG
    GTGGTTGATA ACAGTTTGCC TGGATTTTCT GTCTCTTCTA GTCCACCAGC ATTACCACCC
    AAGAAAAGGC AATCTGCTCC ATCCCCTACC CGGGTGGCTG TGGTAGCCCC CATGAGTCGT
    GCTGTCAGTG GCTCCAGTTT GCCTGTTGGA ATCAATAGGC AGGACTTTGA TGTTGAATGT
    TACACCCAGA GGCGCCTGTC AGGAGGCAGC CGCTCCTGTG GTGGTGAATC TCCTCGCCTG
    TCCCCCTGCA GCAGTACAGG CAAGCTCAGC CGGTCAGACG AGCAGCTGTC CTCCCTAGAC
    AGGGATAGCG GGCAGTGCTC ACGGAACACA AGCTGTGAAA CACTAGATCA CTATGACCCC
    GACTATGAAT TCCTCCAGCA AGACCTCTCC AATGCAGACC AGACCCCTCC ACAGGCAGCC
    TGTAACCTCA GCCCACTGCC GGAGTCCATG GGGGAGTCTG GGCCCCCATT TGTTGGCTAC
    TCTTTCCAGC TGCCTCTGGG CAGCTGTCTC CAGCAGGATG GACAGCAGAC AGATACTCCA
    CCTGCCCTTC CTGAGAAGAA GCGTAGGAGC GCAGTCTCCC AGACCACGGA CAGCTCTGGC
    TGCAGGGTGT CCTATGAGCG ACACCCCTCA CAGTATGACA ACATCTCAGA AGATGATCTG
    CAGAACCCAG CCCAGCCTGT GTCCTACCCG CCCTTTGCTG CTATCCTGCC CTTTCAGCAG
    GGAGCTTCTT CCACCCCTGT TGAGTTTGTG GGTGATTTCA GTGTTCCTGA GTCAGCCGGT
    GACCCAGAAA AGCCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG
    CAACTGCTGG AGGACTACTC AGAGCCACAG CCCTCCATGT TCTACCAGAC ACCGCAGAGT
    GAGCACATCT ACCAGCAGAA GAACAAGATG CTCATGGAGG TCTATGGCTT CAGCGACTCC
    TTCTGTGGTA GTGACTCCAC GCAGGAGCTG GCCCCTCCAC CCGCTTTGCC CCCCAAGCAG
    CGGCAGCTGG AACCGCCATC TGGGAAGGAC GGACATCCCA GAGATCTTTC GGTCAGCAGT
    GCATCTGGGA AGGACAGCAG AGAAAATGGG GAGAGGTCCC CGAAGTCACT GGATGCTCTG
    GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT GAACTGTCCC TCATTGACCA CAATGAAATC
    ATGGCCAGGC TGACACTCAA GCAAGAGGGT GATGATGGAC CAGATGTTCG TGGAGGTTCA
    GGAGACATCT TACTGGTCCA TGCTACTGAG ACAGACAGAA AAGACCTGGT CCTGTACTGT
    GAAGCCTTCC TGACCACCTA CAGAACCTTC ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG
    CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT GCTGACACAT TCAAGAAGCG AGTGAGCAAG
    AACACATTCT TCGTGCTGGT GCGGGTGGTG GACGAGCTCT GCCTGGTGGA GCTGACAGAG
    GAGATCCTGA AGCTGCTGAT GGAGCTAGTC TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC
    TTGGCCAGGG TACTCCGAAA GAACATTCTG GACAAGGTGG ACCAGAAGAA GCTGCTCAGG
    TGTGCCCATT CCGACCAGCC TTTGGCAGCC AGGGGTGTTG CAGCCAGGCC AGGAACCTTG
    CATGATTTCC ACAGCCACGA GATAGCTGAA CAGCTGACAC TGCTGGATGC CGAGCTTTTC
    TATAAGATAG AGATTCCCGA AGTTTTGCTT TGGGCCAAAG AGCAGAATGA AGAGAAGAGC
    CCCAATCTGA CCCAGTTCAC AGAGCACTTC AACAACATGT CCTACTGGGT GCGGTCCGTC
    ATCATGCTGC AGGAGAAGGC CCAGGACCGG GAGAGGCTGC TCCTGAAGTT CATCAAGATC
    ATGAAGCACC TGCGCAAGCT CAACAATTTC AACTCCTACC TGGCCATCCT CTCCGCACTG
    GACTCGGCCC CCATCCGCAG ACTGGAGTGG CAGCGACAGA CCTCAGAGGG CCTGGCTGAG
    TACTGCACGT TGATTGACAG CTCATCCTCC TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG
    GTGGAACCTC CATGTATCCC ATACCTAGGT TTGATCCTGC AAGACCTGAC GTTCGTCCAC
    CTGGGAAACC CAGACTACAT AGACGGGAAG GTCAACTTCT CCAAGCGGTG GCAGCAGTTC
    AACATCCTGG ACAGCATGCG CTGTTTCCAG CAGGTGCACT ATGAAATCCG GAGAAACGAC
    GACATCATAA ACTTCTTCAA TGACTTCAGC GACCACCTGG CCGAGGAAGC CCTATGGGAA
    CTCTCTCTGA AGATCAAGCC TAGGAACATA ACAAGGCGGA AAACAGACCG TGAAGAGAAG
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60565
    Clone ID Related Accession (Same CDS sequence) XM_017592103.1
    Accession Version XM_017592103.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGAGTG AAAACGCCAG CAGTGAGGAA
    GCTGGTGGGG GTGAATATGT CAATCTGTAC TCCTCTGGCC AGACCAGCGA GGAGCTAGCT
    CCCTCTGGAG GAGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447592.1
    CDS3181..6969
    Translation

    Target ORF information:

    RefSeq Version XM_017592103.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592103.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGGCCTC CTATGCTGCT
    TCTTCCTTCT CTGTCTCCCA CTGTGTCCAG CAAACTAAAG TGGCCTTCAC CCCCGAGGAC
    GGCAGTGCCG CTCAGGGCCT CAGTGTATCC GTGTCTAACT CCTTTCTCAA CCGGCACGGC
    AGCTTGCCTG TGCCCTCGTA CAAATCTGTG TTTAGGTCTT ACTCCCAGGA CTTCATGCCT
    CACCACCAAG CCTCCGTCCA ACCTTTCCTT CCGCCTACCT CCTCTTCCTC TCCACATTTC
    CCACCTGTCC ACGCGTCCCA GAGCTCGGAC TTAGCGGTGC CCACCGTAAG CAGTCCGCCT
    CCCAGCACCG TGGACGGGCC TCTCTCGTCT TCTCAGGACA GCAGCTTTCA TGGGAACCCT
    GTCCGCCTTC CTTCCGAAAC CTCTTTCACT GACTCGAGTG AAAACGCCAG CAGTGAGGAA
    GCTGGTGGGG GTGAATATGT CAATCTGTAC TCCTCTGGCC AGACCAGCGA GGAGCTAGCT
    CCCTCTGGAG GAGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60566
    Clone ID Related Accession (Same CDS sequence) XM_008761683.2
    Accession Version XM_008761683.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCTGT CTCCCACTGT
    GTCCAGCAAA CTAAAGTGGC CTTCACCCCC GAGGACGGCA GTGCCGCTCA GGGCCTCAGT
    GTATCCGTGT CTAACTCCTT TCTCAACCGG CACGGCAGCT TGCCTGTGCC CTCGTACAAA
    TCTGTGTTTA GGTCTTACTC CCAGGACTTC ATGCCTCACC ACCAAGCCTC CGTCCAACCT
    TTCCTTCCGC CTACCTCCTC TTCCTCTCCA CATTTCCCAC CTGTCCACGC GTCCCAGAGC
    TCGGACTTAG CGGTGCCCAC CGTAAGCAGT CCGCCTCCCA GCACCGTGGA CGGGCCTCTC
    TCGTCTTCTC AGGACAGCAG CTTTCATGGG AACCCTGTCC GCCTTCCTTC CGAAACCTCT
    TTCACTGACT CGAGTGAAAA CGCCAGCAGT GAGGAAGCTG GTGGGGGTGA ATATGTCAAT
    CTGTACTCCT CTGGCCAGAC CAGCGAGGAG CTAGCTCCCT CTGGAGGAGA ACCGCCATCT
    GGGAAGGACG GACATCCCAG AGATCTTTCG GTCAGCAGTG CATCTGGGAA GGACAGCAGA
    GAAAATGGGG AGAGGTCCCC GAAGTCACTG GATGCTCTGG AGTCAGCCCA GTCAGAAGAG
    GAAGTGGATG AACTGTCCCT CATTGACCAC AATGAAATCA TGGCCAGGCT GACACTCAAG
    CAAGAGGGTG ATGATGGACC AGATGTTCGT GGAGGTTCAG GAGACATCTT ACTGGTCCAT
    GCTACTGAGA CAGACAGAAA AGACCTGGTC CTGTACTGTG AAGCCTTCCT GACCACCTAC
    AGAACCTTCA TCAGCCCCGA GGAGCTCATC AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC
    TCTCCCTTTG CTGACACATT CAAGAAGCGA GTGAGCAAGA ACACATTCTT CGTGCTGGTG
    CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG CTGACAGAGG AGATCCTGAA GCTGCTGATG
    GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG
    AACATTCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCCATTC CGACCAGCCT
    TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA GGAACCTTGC ATGATTTCCA CAGCCACGAG
    ATAGCTGAAC AGCTGACACT GCTGGATGCC GAGCTTTTCT ATAAGATAGA GATTCCCGAA
    GTTTTGCTTT GGGCCAAAGA GCAGAATGAA GAGAAGAGCC CCAATCTGAC CCAGTTCACA
    GAGCACTTCA ACAACATGTC CTACTGGGTG CGGTCCGTCA TCATGCTGCA GGAGAAGGCC
    CAGGACCGGG AGAGGCTGCT CCTGAAGTTC ATCAAGATCA TGAAGCACCT GCGCAAGCTC
    AACAATTTCA ACTCCTACCT GGCCATCCTC TCCGCACTGG ACTCGGCCCC CATCCGCAGA
    CTGGAGTGGC AGCGACAGAC CTCAGAGGGC CTGGCTGAGT ACTGCACGTT GATTGACAGC
    TCATCCTCCT TCCGAGCCTA CCGGGCTGCC CTCTCAGAGG TGGAACCTCC ATGTATCCCA
    TACCTAGGTT TGATCCTGCA AGACCTGACG TTCGTCCACC TGGGAAACCC AGACTACATA
    GACGGGAAGG TCAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGC
    TGTTTCCAGC AGGTGCACTA TGAAATCCGG AGAAACGACG ACATCATAAA CTTCTTCAAT
    GACTTCAGCG ACCACCTGGC CGAGGAAGCC CTATGGGAAC TCTCTCTGAA GATCAAGCCT
    AGGAACATAA CAAGGCGGAA AACAGACCGT GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008759905.2
    CDS3181..6945
    Translation

    Target ORF information:

    RefSeq Version XM_008761683.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008761683.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCTGT CTCCCACTGT
    GTCCAGCAAA CTAAAGTGGC CTTCACCCCC GAGGACGGCA GTGCCGCTCA GGGCCTCAGT
    GTATCCGTGT CTAACTCCTT TCTCAACCGG CACGGCAGCT TGCCTGTGCC CTCGTACAAA
    TCTGTGTTTA GGTCTTACTC CCAGGACTTC ATGCCTCACC ACCAAGCCTC CGTCCAACCT
    TTCCTTCCGC CTACCTCCTC TTCCTCTCCA CATTTCCCAC CTGTCCACGC GTCCCAGAGC
    TCGGACTTAG CGGTGCCCAC CGTAAGCAGT CCGCCTCCCA GCACCGTGGA CGGGCCTCTC
    TCGTCTTCTC AGGACAGCAG CTTTCATGGG AACCCTGTCC GCCTTCCTTC CGAAACCTCT
    TTCACTGACT CGAGTGAAAA CGCCAGCAGT GAGGAAGCTG GTGGGGGTGA ATATGTCAAT
    CTGTACTCCT CTGGCCAGAC CAGCGAGGAG CTAGCTCCCT CTGGAGGAGA ACCGCCATCT
    GGGAAGGACG GACATCCCAG AGATCTTTCG GTCAGCAGTG CATCTGGGAA GGACAGCAGA
    GAAAATGGGG AGAGGTCCCC GAAGTCACTG GATGCTCTGG AGTCAGCCCA GTCAGAAGAG
    GAAGTGGATG AACTGTCCCT CATTGACCAC AATGAAATCA TGGCCAGGCT GACACTCAAG
    CAAGAGGGTG ATGATGGACC AGATGTTCGT GGAGGTTCAG GAGACATCTT ACTGGTCCAT
    GCTACTGAGA CAGACAGAAA AGACCTGGTC CTGTACTGTG AAGCCTTCCT GACCACCTAC
    AGAACCTTCA TCAGCCCCGA GGAGCTCATC AAGAAGCTGC AGTGCCGGTA CGAGAAGTTC
    TCTCCCTTTG CTGACACATT CAAGAAGCGA GTGAGCAAGA ACACATTCTT CGTGCTGGTG
    CGGGTGGTGG ACGAGCTCTG CCTGGTGGAG CTGACAGAGG AGATCCTGAA GCTGCTGATG
    GAGCTAGTCT TCCGCCTAGT GTGCAGTGGA GAGCTCAGCT TGGCCAGGGT ACTCCGAAAG
    AACATTCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCCATTC CGACCAGCCT
    TTGGCAGCCA GGGGTGTTGC AGCCAGGCCA GGAACCTTGC ATGATTTCCA CAGCCACGAG
    ATAGCTGAAC AGCTGACACT GCTGGATGCC GAGCTTTTCT ATAAGATAGA GATTCCCGAA
    GTTTTGCTTT GGGCCAAAGA GCAGAATGAA GAGAAGAGCC CCAATCTGAC CCAGTTCACA
    GAGCACTTCA ACAACATGTC CTACTGGGTG CGGTCCGTCA TCATGCTGCA GGAGAAGGCC
    CAGGACCGGG AGAGGCTGCT CCTGAAGTTC ATCAAGATCA TGAAGCACCT GCGCAAGCTC
    AACAATTTCA ACTCCTACCT GGCCATCCTC TCCGCACTGG ACTCGGCCCC CATCCGCAGA
    CTGGAGTGGC AGCGACAGAC CTCAGAGGGC CTGGCTGAGT ACTGCACGTT GATTGACAGC
    TCATCCTCCT TCCGAGCCTA CCGGGCTGCC CTCTCAGAGG TGGAACCTCC ATGTATCCCA
    TACCTAGGTT TGATCCTGCA AGACCTGACG TTCGTCCACC TGGGAAACCC AGACTACATA
    GACGGGAAGG TCAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTGGA CAGCATGCGC
    TGTTTCCAGC AGGTGCACTA TGAAATCCGG AGAAACGACG ACATCATAAA CTTCTTCAAT
    GACTTCAGCG ACCACCTGGC CGAGGAAGCC CTATGGGAAC TCTCTCTGAA GATCAAGCCT
    AGGAACATAA CAAGGCGGAA AACAGACCGT GAAGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60567
    Clone ID Related Accession (Same CDS sequence) XM_017592104.1
    Accession Version XM_017592104.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447593.1
    CDS3181..6876
    Translation

    Target ORF information:

    RefSeq Version XM_017592104.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592104.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GTACAAATCT GTGTTTAGGT CTTACTCCCA GGACTTCATG
    CCTCACCACC AAGCCTCCGT CCAACCTTTC CTTCCGCCTA CCTCCTCTTC CTCTCCACAT
    TTCCCACCTG TCCACGCGTC CCAGAGCTCG GACTTAGCGG TGCCCACCGT AAGCAGTCCG
    CCTCCCAGCA CCGTGGACGG GCCTCTCTCG TCTTCTCAGG ACAGCAGCTT TCATGGGAAC
    CCTGTCCGCC TTCCTTCCGA AACCTCTTTC ACTGACTCGG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60568
    Clone ID Related Accession (Same CDS sequence) XM_017592105.1
    Accession Version XM_017592105.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTTTG
    CCTGGATTTT CTGTCTCTTC TAGTCCACCA GCATTACCAC CCAAGAAAAG GCAATCTGCT
    CCATCCCCTA CCCGGGTGGC TGTGGTAGCC CCCATGAGTC GTGCTGTCAG TGGCTCCAGT
    TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG
    TCAGGAGGCA GCCGCTCCTG TGGTGGTGAA TCTCCTCGCC TGTCCCCCTG CAGCAGTACA
    GGCAAGCTCA GCCGGTCAGA CGAGCAGCTG TCCTCCCTAG ACAGGGATAG CGGGCAGTGC
    TCACGGAACA CAAGCTGTGA AACACTAGAT CACTATGACC CCGACTATGA ATTCCTCCAG
    CAAGACCTCT CCAATGCAGA CCAGACCCCT CCACAGGCAG CCTGTAACCT CAGCCCACTG
    CCGGAGTCCA TGGGGGAGTC TGGGCCCCCA TTTGTTGGCT ACTCTTTCCA GCTGCCTCTG
    GGCAGCTGTC TCCAGCAGGA TGGACAGCAG ACAGATACTC CACCTGCCCT TCCTGAGAAG
    AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG
    CGACACCCCT CACAGTATGA CAACATCTCA GAAGATGATC TGCAGAACCC AGCCCAGCCT
    GTGTCCTACC CGCCCTTTGC TGCTATCCTG CCCTTTCAGC AGGGAGCTTC TTCCACCCCT
    GTTGAGTTTG TGGGTGATTT CAGTGTTCCT GAGTCAGCCG GTGACCCAGA AAAGCCACCT
    CCTCTACCAG AGAAGAAAAA CAAACACATG CTGGCCTACA TGCAACTGCT GGAGGACTAC
    TCAGAGCCAC AGCCCTCCAT GTTCTACCAG ACACCGCAGA GTGAGCACAT CTACCAGCAG
    AAGAACAAGA TGCTCATGGA GGTCTATGGC TTCAGCGACT CCTTCTGTGG TAGTGACTCC
    ACGCAGGAGC TGGCCCCTCC ACCCGCTTTG CCCCCCAAGC AGCGGCAGCT GCAGGCCTCC
    TATGCTGCTT CTTCCTTCTC TGTCTCCCAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC
    CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTATCCG TGTCTAACTC CTTTCTCAAC
    CGGCACGGCA GCTTGCCTGT GCCCTCGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC
    TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT
    CCACATTTCC CACCTGTCCA CGCGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC
    AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT
    GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGC
    AGTGAGGAAG CTGGTGGGGG TGAATATGTC AATCTGTACT CCTCTGGCCA GACCAGCGAG
    GAGCTAGCTC CCTCTGGAGG AGAACCGCCA TCTGGGAAGG ACGGACATCC CAGAGATCTT
    TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAGAGGTC CCCGAAGTCA
    CTGGATGCTC TGGAGTCAGC CCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC
    CACAATGAAA TCATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG ACCAGATGTT
    CGTGGAGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG
    GTCCTGTACT GTGAAGCCTT CCTGACCACC TACAGAACCT TCATCAGCCC CGAGGAGCTC
    ATCAAGAAGC TGCAGTGCCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC ATTCAAGAAG
    CGAGTGAGCA AGAACACATT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTG
    GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT
    GGAGAGCTCA GCTTGGCCAG GGTACTCCGA AAGAACATTC TGGACAAGGT GGACCAGAAG
    AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG
    CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AACAGCTGAC ACTGCTGGAT
    GCCGAGCTTT TCTATAAGAT AGAGATTCCC GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT
    GAAGAGAAGA GCCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG
    GTGCGGTCCG TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTGAAG
    TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAATT TCAACTCCTA CCTGGCCATC
    CTCTCCGCAC TGGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGACA GACCTCAGAG
    GGCCTGGCTG AGTACTGCAC GTTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT
    GCCCTCTCAG AGGTGGAACC TCCATGTATC CCATACCTAG GTTTGATCCT GCAAGACCTG
    ACGTTCGTCC ACCTGGGAAA CCCAGACTAC ATAGACGGGA AGGTCAACTT CTCCAAGCGG
    TGGCAGCAGT TCAACATCCT GGACAGCATG CGCTGTTTCC AGCAGGTGCA CTATGAAATC
    CGGAGAAACG ACGACATCAT AAACTTCTTC AATGACTTCA GCGACCACCT GGCCGAGGAA
    GCCCTATGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC
    CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447594.1
    CDS3181..6858
    Translation

    Target ORF information:

    RefSeq Version XM_017592105.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTTTG
    CCTGGATTTT CTGTCTCTTC TAGTCCACCA GCATTACCAC CCAAGAAAAG GCAATCTGCT
    CCATCCCCTA CCCGGGTGGC TGTGGTAGCC CCCATGAGTC GTGCTGTCAG TGGCTCCAGT
    TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG
    TCAGGAGGCA GCCGCTCCTG TGGTGGTGAA TCTCCTCGCC TGTCCCCCTG CAGCAGTACA
    GGCAAGCTCA GCCGGTCAGA CGAGCAGCTG TCCTCCCTAG ACAGGGATAG CGGGCAGTGC
    TCACGGAACA CAAGCTGTGA AACACTAGAT CACTATGACC CCGACTATGA ATTCCTCCAG
    CAAGACCTCT CCAATGCAGA CCAGACCCCT CCACAGGCAG CCTGTAACCT CAGCCCACTG
    CCGGAGTCCA TGGGGGAGTC TGGGCCCCCA TTTGTTGGCT ACTCTTTCCA GCTGCCTCTG
    GGCAGCTGTC TCCAGCAGGA TGGACAGCAG ACAGATACTC CACCTGCCCT TCCTGAGAAG
    AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG
    CGACACCCCT CACAGTATGA CAACATCTCA GAAGATGATC TGCAGAACCC AGCCCAGCCT
    GTGTCCTACC CGCCCTTTGC TGCTATCCTG CCCTTTCAGC AGGGAGCTTC TTCCACCCCT
    GTTGAGTTTG TGGGTGATTT CAGTGTTCCT GAGTCAGCCG GTGACCCAGA AAAGCCACCT
    CCTCTACCAG AGAAGAAAAA CAAACACATG CTGGCCTACA TGCAACTGCT GGAGGACTAC
    TCAGAGCCAC AGCCCTCCAT GTTCTACCAG ACACCGCAGA GTGAGCACAT CTACCAGCAG
    AAGAACAAGA TGCTCATGGA GGTCTATGGC TTCAGCGACT CCTTCTGTGG TAGTGACTCC
    ACGCAGGAGC TGGCCCCTCC ACCCGCTTTG CCCCCCAAGC AGCGGCAGCT GCAGGCCTCC
    TATGCTGCTT CTTCCTTCTC TGTCTCCCAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC
    CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTATCCG TGTCTAACTC CTTTCTCAAC
    CGGCACGGCA GCTTGCCTGT GCCCTCGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC
    TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT
    CCACATTTCC CACCTGTCCA CGCGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC
    AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT
    GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGC
    AGTGAGGAAG CTGGTGGGGG TGAATATGTC AATCTGTACT CCTCTGGCCA GACCAGCGAG
    GAGCTAGCTC CCTCTGGAGG AGAACCGCCA TCTGGGAAGG ACGGACATCC CAGAGATCTT
    TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAGAGGTC CCCGAAGTCA
    CTGGATGCTC TGGAGTCAGC CCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC
    CACAATGAAA TCATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG ACCAGATGTT
    CGTGGAGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG
    GTCCTGTACT GTGAAGCCTT CCTGACCACC TACAGAACCT TCATCAGCCC CGAGGAGCTC
    ATCAAGAAGC TGCAGTGCCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC ATTCAAGAAG
    CGAGTGAGCA AGAACACATT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTG
    GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT
    GGAGAGCTCA GCTTGGCCAG GGTACTCCGA AAGAACATTC TGGACAAGGT GGACCAGAAG
    AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG
    CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AACAGCTGAC ACTGCTGGAT
    GCCGAGCTTT TCTATAAGAT AGAGATTCCC GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT
    GAAGAGAAGA GCCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG
    GTGCGGTCCG TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTGAAG
    TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAATT TCAACTCCTA CCTGGCCATC
    CTCTCCGCAC TGGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGACA GACCTCAGAG
    GGCCTGGCTG AGTACTGCAC GTTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT
    GCCCTCTCAG AGGTGGAACC TCCATGTATC CCATACCTAG GTTTGATCCT GCAAGACCTG
    ACGTTCGTCC ACCTGGGAAA CCCAGACTAC ATAGACGGGA AGGTCAACTT CTCCAAGCGG
    TGGCAGCAGT TCAACATCCT GGACAGCATG CGCTGTTTCC AGCAGGTGCA CTATGAAATC
    CGGAGAAACG ACGACATCAT AAACTTCTTC AATGACTTCA GCGACCACCT GGCCGAGGAA
    GCCCTATGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC
    CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60569
    Clone ID Related Accession (Same CDS sequence) XM_008761684.2
    Accession Version XM_008761684.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCTGT CTCCCACTGT
    GTCCAGCAAA CTAAAGTGGC CTTCACCCCC GAGGACGGCA GTGCCGCTCA GGGCCTCAGT
    GTATCCGTGT CTAACTCCTT TCTCAACCGG CACGGCAGCT TGCCTGTGCC CTCGTACAAA
    TCTGTGTTTA GGTCTTACTC CCAGGACTTC ATGCCTCACC ACCAAGCCTC CGTCCAACCT
    TTCCTTCCGC CTACCTCCTC TTCCTCTCCA CATTTCCCAC CTGTCCACGC GTCCCAGAGC
    TCGGACTTAG CGGTGCCCAC CGTAAGCAGT CCGCCTCCCA GCACCGTGGA CGGGCCTCTC
    TCGTCTTCTC AGGACAGCAG CTTTCATGGG AACCCTGTCC GCCTTCCTTC CGAAACCTCT
    TTCACTGACT CGGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008759906.2
    CDS3180..6848
    Translation

    Target ORF information:

    RefSeq Version XM_008761684.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008761684.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCTGT CTCCCACTGT
    GTCCAGCAAA CTAAAGTGGC CTTCACCCCC GAGGACGGCA GTGCCGCTCA GGGCCTCAGT
    GTATCCGTGT CTAACTCCTT TCTCAACCGG CACGGCAGCT TGCCTGTGCC CTCGTACAAA
    TCTGTGTTTA GGTCTTACTC CCAGGACTTC ATGCCTCACC ACCAAGCCTC CGTCCAACCT
    TTCCTTCCGC CTACCTCCTC TTCCTCTCCA CATTTCCCAC CTGTCCACGC GTCCCAGAGC
    TCGGACTTAG CGGTGCCCAC CGTAAGCAGT CCGCCTCCCA GCACCGTGGA CGGGCCTCTC
    TCGTCTTCTC AGGACAGCAG CTTTCATGGG AACCCTGTCC GCCTTCCTTC CGAAACCTCT
    TTCACTGACT CGGAACCGCC ATCTGGGAAG GACGGACATC CCAGAGATCT TTCGGTCAGC
    AGTGCATCTG GGAAGGACAG CAGAGAAAAT GGGGAGAGGT CCCCGAAGTC ACTGGATGCT
    CTGGAGTCAG CCCAGTCAGA AGAGGAAGTG GATGAACTGT CCCTCATTGA CCACAATGAA
    ATCATGGCCA GGCTGACACT CAAGCAAGAG GGTGATGATG GACCAGATGT TCGTGGAGGT
    TCAGGAGACA TCTTACTGGT CCATGCTACT GAGACAGACA GAAAAGACCT GGTCCTGTAC
    TGTGAAGCCT TCCTGACCAC CTACAGAACC TTCATCAGCC CCGAGGAGCT CATCAAGAAG
    CTGCAGTGCC GGTACGAGAA GTTCTCTCCC TTTGCTGACA CATTCAAGAA GCGAGTGAGC
    AAGAACACAT TCTTCGTGCT GGTGCGGGTG GTGGACGAGC TCTGCCTGGT GGAGCTGACA
    GAGGAGATCC TGAAGCTGCT GATGGAGCTA GTCTTCCGCC TAGTGTGCAG TGGAGAGCTC
    AGCTTGGCCA GGGTACTCCG AAAGAACATT CTGGACAAGG TGGACCAGAA GAAGCTGCTC
    AGGTGTGCCC ATTCCGACCA GCCTTTGGCA GCCAGGGGTG TTGCAGCCAG GCCAGGAACC
    TTGCATGATT TCCACAGCCA CGAGATAGCT GAACAGCTGA CACTGCTGGA TGCCGAGCTT
    TTCTATAAGA TAGAGATTCC CGAAGTTTTG CTTTGGGCCA AAGAGCAGAA TGAAGAGAAG
    AGCCCCAATC TGACCCAGTT CACAGAGCAC TTCAACAACA TGTCCTACTG GGTGCGGTCC
    GTCATCATGC TGCAGGAGAA GGCCCAGGAC CGGGAGAGGC TGCTCCTGAA GTTCATCAAG
    ATCATGAAGC ACCTGCGCAA GCTCAACAAT TTCAACTCCT ACCTGGCCAT CCTCTCCGCA
    CTGGACTCGG CCCCCATCCG CAGACTGGAG TGGCAGCGAC AGACCTCAGA GGGCCTGGCT
    GAGTACTGCA CGTTGATTGA CAGCTCATCC TCCTTCCGAG CCTACCGGGC TGCCCTCTCA
    GAGGTGGAAC CTCCATGTAT CCCATACCTA GGTTTGATCC TGCAAGACCT GACGTTCGTC
    CACCTGGGAA ACCCAGACTA CATAGACGGG AAGGTCAACT TCTCCAAGCG GTGGCAGCAG
    TTCAACATCC TGGACAGCAT GCGCTGTTTC CAGCAGGTGC ACTATGAAAT CCGGAGAAAC
    GACGACATCA TAAACTTCTT CAATGACTTC AGCGACCACC TGGCCGAGGA AGCCCTATGG
    GAACTCTCTC TGAAGATCAA GCCTAGGAAC ATAACAAGGC GGAAAACAGA CCGTGAAGAG
    AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60570
    Clone ID Related Accession (Same CDS sequence) XM_008761685.2
    Accession Version XM_008761685.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA
    CCAGCATTAC CACCCAAGAA AAGGCAATCT GCTCCATCCC CTACCCGGGT GGCTGTGGTA
    GCCCCCATGA GTCGTGCTGT CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC
    TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGTGGTGGT
    GAATCTCCTC GCCTGTCCCC CTGCAGCAGT ACAGGCAAGC TCAGCCGGTC AGACGAGCAG
    CTGTCCTCCC TAGACAGGGA TAGCGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA
    GATCACTATG ACCCCGACTA TGAATTCCTC CAGCAAGACC TCTCCAATGC AGACCAGACC
    CCTCCACAGG CAGCCTGTAA CCTCAGCCCA CTGCCGGAGT CCATGGGGGA GTCTGGGCCC
    CCATTTGTTG GCTACTCTTT CCAGCTGCCT CTGGGCAGCT GTCTCCAGCA GGATGGACAG
    CAGACAGATA CTCCACCTGC CCTTCCTGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC
    TCAGAAGATG ATCTGCAGAA CCCAGCCCAG CCTGTGTCCT ACCCGCCCTT TGCTGCTATC
    CTGCCCTTTC AGCAGGGAGC TTCTTCCACC CCTGTTGAGT TTGTGGGTGA TTTCAGTGTT
    CCTGAGTCAG CCGGTGACCC AGAAAAGCCA CCTCCTCTAC CAGAGAAGAA AAACAAACAC
    ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC
    CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTCTAT
    GGCTTCAGCG ACTCCTTCTG TGGTAGTGAC TCCACGCAGG AGCTGGCCCC TCCACCCGCT
    TTGCCCCCCA AGCAGCGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC
    CACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC
    CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG
    TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC
    CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACGCGTCC
    CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG
    CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA
    ACCTCTTTCA CTGACTCGAG TGAAAACGCC AGCAGTGAGG AAGCTGGTGG GGGTGAATAT
    GTCAATCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTAG CTCCCTCTGG AGGAGAACCG
    CCATCTGGGA AGGACGGACA TCCCAGAGAT CTTTCGGTCA GCAGTGCATC TGGGAAGGAC
    AGCAGAGAAA ATGGGGAGAG GTCCCCGAAG TCACTGGATG CTCTGGAGTC AGCCCAGTCA
    GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATCATGGC CAGGCTGACA
    CTCAAGCAAG AGGGTGATGA TGGACCAGAT GTTCGTGGAG GTTCAGGAGA CATCTTACTG
    GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTCCTGT ACTGTGAAGC CTTCCTGACC
    ACCTACAGAA CCTTCATCAG CCCCGAGGAG CTCATCAAGA AGCTGCAGTG CCGGTACGAG
    AAGTTCTCTC CCTTTGCTGA CACATTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTCGTG
    CTGGTGCGGG TGGTGGACGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG
    CTGATGGAGC TAGTCTTCCG CCTAGTGTGC AGTGGAGAGC TCAGCTTGGC CAGGGTACTC
    CGAAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC
    CAGCCTTTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC
    CACGAGATAG CTGAACAGCT GACACTGCTG GATGCCGAGC TTTTCTATAA GATAGAGATT
    CCCGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAAGAGA AGAGCCCCAA TCTGACCCAG
    TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCGTCATCAT GCTGCAGGAG
    AAGGCCCAGG ACCGGGAGAG GCTGCTCCTG AAGTTCATCA AGATCATGAA GCACCTGCGC
    AAGCTCAACA ATTTCAACTC CTACCTGGCC ATCCTCTCCG CACTGGACTC GGCCCCCATC
    CGCAGACTGG AGTGGCAGCG ACAGACCTCA GAGGGCCTGG CTGAGTACTG CACGTTGATT
    GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCCCTCT CAGAGGTGGA ACCTCCATGT
    ATCCCATACC TAGGTTTGAT CCTGCAAGAC CTGACGTTCG TCCACCTGGG AAACCCAGAC
    TACATAGACG GGAAGGTCAA CTTCTCCAAG CGGTGGCAGC AGTTCAACAT CCTGGACAGC
    ATGCGCTGTT TCCAGCAGGT GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAACTTC
    TTCAATGACT TCAGCGACCA CCTGGCCGAG GAAGCCCTAT GGGAACTCTC TCTGAAGATC
    AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGTGAAG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008759907.2
    CDS3181..6831
    Translation

    Target ORF information:

    RefSeq Version XM_008761685.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008761685.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA
    CCAGCATTAC CACCCAAGAA AAGGCAATCT GCTCCATCCC CTACCCGGGT GGCTGTGGTA
    GCCCCCATGA GTCGTGCTGT CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC
    TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGTGGTGGT
    GAATCTCCTC GCCTGTCCCC CTGCAGCAGT ACAGGCAAGC TCAGCCGGTC AGACGAGCAG
    CTGTCCTCCC TAGACAGGGA TAGCGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA
    GATCACTATG ACCCCGACTA TGAATTCCTC CAGCAAGACC TCTCCAATGC AGACCAGACC
    CCTCCACAGG CAGCCTGTAA CCTCAGCCCA CTGCCGGAGT CCATGGGGGA GTCTGGGCCC
    CCATTTGTTG GCTACTCTTT CCAGCTGCCT CTGGGCAGCT GTCTCCAGCA GGATGGACAG
    CAGACAGATA CTCCACCTGC CCTTCCTGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC
    TCAGAAGATG ATCTGCAGAA CCCAGCCCAG CCTGTGTCCT ACCCGCCCTT TGCTGCTATC
    CTGCCCTTTC AGCAGGGAGC TTCTTCCACC CCTGTTGAGT TTGTGGGTGA TTTCAGTGTT
    CCTGAGTCAG CCGGTGACCC AGAAAAGCCA CCTCCTCTAC CAGAGAAGAA AAACAAACAC
    ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC
    CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTCTAT
    GGCTTCAGCG ACTCCTTCTG TGGTAGTGAC TCCACGCAGG AGCTGGCCCC TCCACCCGCT
    TTGCCCCCCA AGCAGCGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC
    CACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC
    CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG
    TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC
    CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACGCGTCC
    CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG
    CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA
    ACCTCTTTCA CTGACTCGAG TGAAAACGCC AGCAGTGAGG AAGCTGGTGG GGGTGAATAT
    GTCAATCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTAG CTCCCTCTGG AGGAGAACCG
    CCATCTGGGA AGGACGGACA TCCCAGAGAT CTTTCGGTCA GCAGTGCATC TGGGAAGGAC
    AGCAGAGAAA ATGGGGAGAG GTCCCCGAAG TCACTGGATG CTCTGGAGTC AGCCCAGTCA
    GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATCATGGC CAGGCTGACA
    CTCAAGCAAG AGGGTGATGA TGGACCAGAT GTTCGTGGAG GTTCAGGAGA CATCTTACTG
    GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTCCTGT ACTGTGAAGC CTTCCTGACC
    ACCTACAGAA CCTTCATCAG CCCCGAGGAG CTCATCAAGA AGCTGCAGTG CCGGTACGAG
    AAGTTCTCTC CCTTTGCTGA CACATTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTCGTG
    CTGGTGCGGG TGGTGGACGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG
    CTGATGGAGC TAGTCTTCCG CCTAGTGTGC AGTGGAGAGC TCAGCTTGGC CAGGGTACTC
    CGAAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC
    CAGCCTTTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC
    CACGAGATAG CTGAACAGCT GACACTGCTG GATGCCGAGC TTTTCTATAA GATAGAGATT
    CCCGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAAGAGA AGAGCCCCAA TCTGACCCAG
    TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCGTCATCAT GCTGCAGGAG
    AAGGCCCAGG ACCGGGAGAG GCTGCTCCTG AAGTTCATCA AGATCATGAA GCACCTGCGC
    AAGCTCAACA ATTTCAACTC CTACCTGGCC ATCCTCTCCG CACTGGACTC GGCCCCCATC
    CGCAGACTGG AGTGGCAGCG ACAGACCTCA GAGGGCCTGG CTGAGTACTG CACGTTGATT
    GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCCCTCT CAGAGGTGGA ACCTCCATGT
    ATCCCATACC TAGGTTTGAT CCTGCAAGAC CTGACGTTCG TCCACCTGGG AAACCCAGAC
    TACATAGACG GGAAGGTCAA CTTCTCCAAG CGGTGGCAGC AGTTCAACAT CCTGGACAGC
    ATGCGCTGTT TCCAGCAGGT GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAACTTC
    TTCAATGACT TCAGCGACCA CCTGGCCGAG GAAGCCCTAT GGGAACTCTC TCTGAAGATC
    AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGTGAAG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60571
    Clone ID Related Accession (Same CDS sequence) XM_017592106.1
    Accession Version XM_017592106.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGAGTGAAA ACGCCAGCAG TGAGGAAGCT GGTGGGGGTG AATATGTCAA TCTGTACTCC
    TCTGGCCAGA CCAGCGAGGA GCTAGCTCCC TCTGGAGGAG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447595.1
    CDS3181..6816
    Translation

    Target ORF information:

    RefSeq Version XM_017592106.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGTACAA ATCTGTGTTT
    AGGTCTTACT CCCAGGACTT CATGCCTCAC CACCAAGCCT CCGTCCAACC TTTCCTTCCG
    CCTACCTCCT CTTCCTCTCC ACATTTCCCA CCTGTCCACG CGTCCCAGAG CTCGGACTTA
    GCGGTGCCCA CCGTAAGCAG TCCGCCTCCC AGCACCGTGG ACGGGCCTCT CTCGTCTTCT
    CAGGACAGCA GCTTTCATGG GAACCCTGTC CGCCTTCCTT CCGAAACCTC TTTCACTGAC
    TCGAGTGAAA ACGCCAGCAG TGAGGAAGCT GGTGGGGGTG AATATGTCAA TCTGTACTCC
    TCTGGCCAGA CCAGCGAGGA GCTAGCTCCC TCTGGAGGAG AACCGCCATC TGGGAAGGAC
    GGACATCCCA GAGATCTTTC GGTCAGCAGT GCATCTGGGA AGGACAGCAG AGAAAATGGG
    GAGAGGTCCC CGAAGTCACT GGATGCTCTG GAGTCAGCCC AGTCAGAAGA GGAAGTGGAT
    GAACTGTCCC TCATTGACCA CAATGAAATC ATGGCCAGGC TGACACTCAA GCAAGAGGGT
    GATGATGGAC CAGATGTTCG TGGAGGTTCA GGAGACATCT TACTGGTCCA TGCTACTGAG
    ACAGACAGAA AAGACCTGGT CCTGTACTGT GAAGCCTTCC TGACCACCTA CAGAACCTTC
    ATCAGCCCCG AGGAGCTCAT CAAGAAGCTG CAGTGCCGGT ACGAGAAGTT CTCTCCCTTT
    GCTGACACAT TCAAGAAGCG AGTGAGCAAG AACACATTCT TCGTGCTGGT GCGGGTGGTG
    GACGAGCTCT GCCTGGTGGA GCTGACAGAG GAGATCCTGA AGCTGCTGAT GGAGCTAGTC
    TTCCGCCTAG TGTGCAGTGG AGAGCTCAGC TTGGCCAGGG TACTCCGAAA GAACATTCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCCATT CCGACCAGCC TTTGGCAGCC
    AGGGGTGTTG CAGCCAGGCC AGGAACCTTG CATGATTTCC ACAGCCACGA GATAGCTGAA
    CAGCTGACAC TGCTGGATGC CGAGCTTTTC TATAAGATAG AGATTCCCGA AGTTTTGCTT
    TGGGCCAAAG AGCAGAATGA AGAGAAGAGC CCCAATCTGA CCCAGTTCAC AGAGCACTTC
    AACAACATGT CCTACTGGGT GCGGTCCGTC ATCATGCTGC AGGAGAAGGC CCAGGACCGG
    GAGAGGCTGC TCCTGAAGTT CATCAAGATC ATGAAGCACC TGCGCAAGCT CAACAATTTC
    AACTCCTACC TGGCCATCCT CTCCGCACTG GACTCGGCCC CCATCCGCAG ACTGGAGTGG
    CAGCGACAGA CCTCAGAGGG CCTGGCTGAG TACTGCACGT TGATTGACAG CTCATCCTCC
    TTCCGAGCCT ACCGGGCTGC CCTCTCAGAG GTGGAACCTC CATGTATCCC ATACCTAGGT
    TTGATCCTGC AAGACCTGAC GTTCGTCCAC CTGGGAAACC CAGACTACAT AGACGGGAAG
    GTCAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTGG ACAGCATGCG CTGTTTCCAG
    CAGGTGCACT ATGAAATCCG GAGAAACGAC GACATCATAA ACTTCTTCAA TGACTTCAGC
    GACCACCTGG CCGAGGAAGC CCTATGGGAA CTCTCTCTGA AGATCAAGCC TAGGAACATA
    ACAAGGCGGA AAACAGACCG TGAAGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60572
    Clone ID Related Accession (Same CDS sequence) XM_008761686.2
    Accession Version XM_008761686.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA
    CCAGCATTAC CACCCAAGAA AAGGCAATCT GCTCCATCCC CTACCCGGGT GGCTGTGGTA
    GCCCCCATGA GTCGTGCTGT CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC
    TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGTGGTGGT
    GAATCTCCTC GCCTGTCCCC CTGCAGCAGT ACAGGCAAGC TCAGCCGGTC AGACGAGCAG
    CTGTCCTCCC TAGACAGGGA TAGCGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA
    GATCACTATG ACCCCGACTA TGAATTCCTC CAGCAAGACC TCTCCAATGC AGACCAGACC
    CCTCCACAGG CAGCCTGTAA CCTCAGCCCA CTGCCGGAGT CCATGGGGGA GTCTGGGCCC
    CCATTTGTTG GCTACTCTTT CCAGCTGCCT CTGGGCAGCT GTCTCCAGCA GGATGGACAG
    CAGACAGATA CTCCACCTGC CCTTCCTGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC
    TCAGAAGATG ATCTGCAGAA CCCAGCCCAG CCTGTGTCCT ACCCGCCCTT TGCTGCTATC
    CTGCCCTTTC AGCAGGGAGC TTCTTCCACC CCTGTTGAGT TTGTGGGTGA TTTCAGTGTT
    CCTGAGTCAG CCGGTGACCC AGAAAAGCCA CCTCCTCTAC CAGAGAAGAA AAACAAACAC
    ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC
    CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTCTAT
    GGCTTCAGCG ACTCCTTCTG TGGTAGTGAC TCCACGCAGG AGCTGGCCCC TCCACCCGCT
    TTGCCCCCCA AGCAGCGGCA GCTGGCCTCC TATGCTGCTT CTTCCTTCTC TGTCTCCCAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC
    AGTGTATCCG TGTCTAACTC CTTTCTCAAC CGGCACGGCA GCTTGCCTGT GCCCTCGTAC
    AAATCTGTGT TTAGGTCTTA CTCCCAGGAC TTCATGCCTC ACCACCAAGC CTCCGTCCAA
    CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGTCCA CGCGTCCCAG
    AGCTCGGACT TAGCGGTGCC CACCGTAAGC AGTCCGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGACAG CAGCTTTCAT GGGAACCCTG TCCGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008759908.2
    CDS3180..6731
    Translation

    Target ORF information:

    RefSeq Version XM_008761686.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008761686.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC
    AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG
    GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT
    GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCTTGCT ATAGCCGAGT GTACCAGAGC
    CTTGCCAACC TTATCCGCTG GTCTGACCAG GTGATGCTCG AGGGTGTGAA CTCAGAAGAT
    AAGGAGATGG TGACGACTGT GAAGGGTGTC ATCAAAGCTG TGCTGGATGG AGTGAAGGAA
    CTGGTGAGGC TAACCATTGA GAAGCAAGGC CGGCCATCAC CAACAAGCCC AGTGAAGCCC
    AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA ACGAGAAATG
    GAGATTCTGA ACAAGACAAG TGTGTCACCA TCCACTGAAC TGCTCCCAGA CTCCACCAGT
    GAAGAGGTTG CGCCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA
    CCAGCATTAC CACCCAAGAA AAGGCAATCT GCTCCATCCC CTACCCGGGT GGCTGTGGTA
    GCCCCCATGA GTCGTGCTGT CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC
    TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGTGGTGGT
    GAATCTCCTC GCCTGTCCCC CTGCAGCAGT ACAGGCAAGC TCAGCCGGTC AGACGAGCAG
    CTGTCCTCCC TAGACAGGGA TAGCGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA
    GATCACTATG ACCCCGACTA TGAATTCCTC CAGCAAGACC TCTCCAATGC AGACCAGACC
    CCTCCACAGG CAGCCTGTAA CCTCAGCCCA CTGCCGGAGT CCATGGGGGA GTCTGGGCCC
    CCATTTGTTG GCTACTCTTT CCAGCTGCCT CTGGGCAGCT GTCTCCAGCA GGATGGACAG
    CAGACAGATA CTCCACCTGC CCTTCCTGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC
    TCAGAAGATG ATCTGCAGAA CCCAGCCCAG CCTGTGTCCT ACCCGCCCTT TGCTGCTATC
    CTGCCCTTTC AGCAGGGAGC TTCTTCCACC CCTGTTGAGT TTGTGGGTGA TTTCAGTGTT
    CCTGAGTCAG CCGGTGACCC AGAAAAGCCA CCTCCTCTAC CAGAGAAGAA AAACAAACAC
    ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC
    CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTCTAT
    GGCTTCAGCG ACTCCTTCTG TGGTAGTGAC TCCACGCAGG AGCTGGCCCC TCCACCCGCT
    TTGCCCCCCA AGCAGCGGCA GCTGGCCTCC TATGCTGCTT CTTCCTTCTC TGTCTCCCAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC
    AGTGTATCCG TGTCTAACTC CTTTCTCAAC CGGCACGGCA GCTTGCCTGT GCCCTCGTAC
    AAATCTGTGT TTAGGTCTTA CTCCCAGGAC TTCATGCCTC ACCACCAAGC CTCCGTCCAA
    CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGTCCA CGCGTCCCAG
    AGCTCGGACT TAGCGGTGCC CACCGTAAGC AGTCCGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGACAG CAGCTTTCAT GGGAACCCTG TCCGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGGAACC GCCATCTGGG AAGGACGGAC ATCCCAGAGA TCTTTCGGTC
    AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA AATGGGGAGA GGTCCCCGAA GTCACTGGAT
    GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA GTGGATGAAC TGTCCCTCAT TGACCACAAT
    GAAATCATGG CCAGGCTGAC ACTCAAGCAA GAGGGTGATG ATGGACCAGA TGTTCGTGGA
    GGTTCAGGAG ACATCTTACT GGTCCATGCT ACTGAGACAG ACAGAAAAGA CCTGGTCCTG
    TACTGTGAAG CCTTCCTGAC CACCTACAGA ACCTTCATCA GCCCCGAGGA GCTCATCAAG
    AAGCTGCAGT GCCGGTACGA GAAGTTCTCT CCCTTTGCTG ACACATTCAA GAAGCGAGTG
    AGCAAGAACA CATTCTTCGT GCTGGTGCGG GTGGTGGACG AGCTCTGCCT GGTGGAGCTG
    ACAGAGGAGA TCCTGAAGCT GCTGATGGAG CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG
    CTCAGCTTGG CCAGGGTACT CCGAAAGAAC ATTCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCCATTCCGA CCAGCCTTTG GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA
    ACCTTGCATG ATTTCCACAG CCACGAGATA GCTGAACAGC TGACACTGCT GGATGCCGAG
    CTTTTCTATA AGATAGAGAT TCCCGAAGTT TTGCTTTGGG CCAAAGAGCA GAATGAAGAG
    AAGAGCCCCA ATCTGACCCA GTTCACAGAG CACTTCAACA ACATGTCCTA CTGGGTGCGG
    TCCGTCATCA TGCTGCAGGA GAAGGCCCAG GACCGGGAGA GGCTGCTCCT GAAGTTCATC
    AAGATCATGA AGCACCTGCG CAAGCTCAAC AATTTCAACT CCTACCTGGC CATCCTCTCC
    GCACTGGACT CGGCCCCCAT CCGCAGACTG GAGTGGCAGC GACAGACCTC AGAGGGCCTG
    GCTGAGTACT GCACGTTGAT TGACAGCTCA TCCTCCTTCC GAGCCTACCG GGCTGCCCTC
    TCAGAGGTGG AACCTCCATG TATCCCATAC CTAGGTTTGA TCCTGCAAGA CCTGACGTTC
    GTCCACCTGG GAAACCCAGA CTACATAGAC GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG
    CAGTTCAACA TCCTGGACAG CATGCGCTGT TTCCAGCAGG TGCACTATGA AATCCGGAGA
    AACGACGACA TCATAAACTT CTTCAATGAC TTCAGCGACC ACCTGGCCGA GGAAGCCCTA
    TGGGAACTCT CTCTGAAGAT CAAGCCTAGG AACATAACAA GGCGGAAAAC AGACCGTGAA
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60573
    Clone ID Related Accession (Same CDS sequence) XM_017592107.1
    Accession Version XM_017592107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GGAACCGCCA TCTGGGAAGG ACGGACATCC CAGAGATCTT
    TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAGAGGTC CCCGAAGTCA
    CTGGATGCTC TGGAGTCAGC CCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC
    CACAATGAAA TCATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG ACCAGATGTT
    CGTGGAGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG
    GTCCTGTACT GTGAAGCCTT CCTGACCACC TACAGAACCT TCATCAGCCC CGAGGAGCTC
    ATCAAGAAGC TGCAGTGCCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC ATTCAAGAAG
    CGAGTGAGCA AGAACACATT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTG
    GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT
    GGAGAGCTCA GCTTGGCCAG GGTACTCCGA AAGAACATTC TGGACAAGGT GGACCAGAAG
    AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG
    CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AACAGCTGAC ACTGCTGGAT
    GCCGAGCTTT TCTATAAGAT AGAGATTCCC GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT
    GAAGAGAAGA GCCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG
    GTGCGGTCCG TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTGAAG
    TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAATT TCAACTCCTA CCTGGCCATC
    CTCTCCGCAC TGGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGACA GACCTCAGAG
    GGCCTGGCTG AGTACTGCAC GTTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT
    GCCCTCTCAG AGGTGGAACC TCCATGTATC CCATACCTAG GTTTGATCCT GCAAGACCTG
    ACGTTCGTCC ACCTGGGAAA CCCAGACTAC ATAGACGGGA AGGTCAACTT CTCCAAGCGG
    TGGCAGCAGT TCAACATCCT GGACAGCATG CGCTGTTTCC AGCAGGTGCA CTATGAAATC
    CGGAGAAACG ACGACATCAT AAACTTCTTC AATGACTTCA GCGACCACCT GGCCGAGGAA
    GCCCTATGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC
    CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447596.1
    CDS3180..6617
    Translation

    Target ORF information:

    RefSeq Version XM_017592107.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTATGCT
    GCTTCTTCCT TCTCTGTCTC CCACTGTGTC CAGCAAACTA AAGTGGCCTT CACCCCCGAG
    GACGGCAGTG CCGCTCAGGG CCTCAGTGTA TCCGTGTCTA ACTCCTTTCT CAACCGGCAC
    GGCAGCTTGC CTGTGCCCTC GGAACCGCCA TCTGGGAAGG ACGGACATCC CAGAGATCTT
    TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAGAGGTC CCCGAAGTCA
    CTGGATGCTC TGGAGTCAGC CCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC
    CACAATGAAA TCATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG ACCAGATGTT
    CGTGGAGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG
    GTCCTGTACT GTGAAGCCTT CCTGACCACC TACAGAACCT TCATCAGCCC CGAGGAGCTC
    ATCAAGAAGC TGCAGTGCCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC ATTCAAGAAG
    CGAGTGAGCA AGAACACATT CTTCGTGCTG GTGCGGGTGG TGGACGAGCT CTGCCTGGTG
    GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT
    GGAGAGCTCA GCTTGGCCAG GGTACTCCGA AAGAACATTC TGGACAAGGT GGACCAGAAG
    AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG
    CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AACAGCTGAC ACTGCTGGAT
    GCCGAGCTTT TCTATAAGAT AGAGATTCCC GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT
    GAAGAGAAGA GCCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG
    GTGCGGTCCG TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTGAAG
    TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAATT TCAACTCCTA CCTGGCCATC
    CTCTCCGCAC TGGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGACA GACCTCAGAG
    GGCCTGGCTG AGTACTGCAC GTTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT
    GCCCTCTCAG AGGTGGAACC TCCATGTATC CCATACCTAG GTTTGATCCT GCAAGACCTG
    ACGTTCGTCC ACCTGGGAAA CCCAGACTAC ATAGACGGGA AGGTCAACTT CTCCAAGCGG
    TGGCAGCAGT TCAACATCCT GGACAGCATG CGCTGTTTCC AGCAGGTGCA CTATGAAATC
    CGGAGAAACG ACGACATCAT AAACTTCTTC AATGACTTCA GCGACCACCT GGCCGAGGAA
    GCCCTATGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC
    CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60574
    Clone ID Related Accession (Same CDS sequence) XM_017592108.1
    Accession Version XM_017592108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGC CTCCTATGCT GCTTCTTCCT TCTCTGTCTC CCACTGTGTC
    CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTA
    TCCGTGTCTA ACTCCTTTCT CAACCGGCAC GGCAGCTTGC CTGTGCCCTC GGAACCGCCA
    TCTGGGAAGG ACGGACATCC CAGAGATCTT TCGGTCAGCA GTGCATCTGG GAAGGACAGC
    AGAGAAAATG GGGAGAGGTC CCCGAAGTCA CTGGATGCTC TGGAGTCAGC CCAGTCAGAA
    GAGGAAGTGG ATGAACTGTC CCTCATTGAC CACAATGAAA TCATGGCCAG GCTGACACTC
    AAGCAAGAGG GTGATGATGG ACCAGATGTT CGTGGAGGTT CAGGAGACAT CTTACTGGTC
    CATGCTACTG AGACAGACAG AAAAGACCTG GTCCTGTACT GTGAAGCCTT CCTGACCACC
    TACAGAACCT TCATCAGCCC CGAGGAGCTC ATCAAGAAGC TGCAGTGCCG GTACGAGAAG
    TTCTCTCCCT TTGCTGACAC ATTCAAGAAG CGAGTGAGCA AGAACACATT CTTCGTGCTG
    GTGCGGGTGG TGGACGAGCT CTGCCTGGTG GAGCTGACAG AGGAGATCCT GAAGCTGCTG
    ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT GGAGAGCTCA GCTTGGCCAG GGTACTCCGA
    AAGAACATTC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCCA TTCCGACCAG
    CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG CCAGGAACCT TGCATGATTT CCACAGCCAC
    GAGATAGCTG AACAGCTGAC ACTGCTGGAT GCCGAGCTTT TCTATAAGAT AGAGATTCCC
    GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT GAAGAGAAGA GCCCCAATCT GACCCAGTTC
    ACAGAGCACT TCAACAACAT GTCCTACTGG GTGCGGTCCG TCATCATGCT GCAGGAGAAG
    GCCCAGGACC GGGAGAGGCT GCTCCTGAAG TTCATCAAGA TCATGAAGCA CCTGCGCAAG
    CTCAACAATT TCAACTCCTA CCTGGCCATC CTCTCCGCAC TGGACTCGGC CCCCATCCGC
    AGACTGGAGT GGCAGCGACA GACCTCAGAG GGCCTGGCTG AGTACTGCAC GTTGATTGAC
    AGCTCATCCT CCTTCCGAGC CTACCGGGCT GCCCTCTCAG AGGTGGAACC TCCATGTATC
    CCATACCTAG GTTTGATCCT GCAAGACCTG ACGTTCGTCC ACCTGGGAAA CCCAGACTAC
    ATAGACGGGA AGGTCAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT GGACAGCATG
    CGCTGTTTCC AGCAGGTGCA CTATGAAATC CGGAGAAACG ACGACATCAT AAACTTCTTC
    AATGACTTCA GCGACCACCT GGCCGAGGAA GCCCTATGGG AACTCTCTCT GAAGATCAAG
    CCTAGGAACA TAACAAGGCG GAAAACAGAC CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447597.1
    CDS3180..6587
    Translation

    Target ORF information:

    RefSeq Version XM_017592108.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA
    TTACCACCCA AGAAAAGGCA ATCTGCTCCA TCCCCTACCC GGGTGGCTGT GGTAGCCCCC
    ATGAGTCGTG CTGTCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT
    GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGTGG TGGTGAATCT
    CCTCGCCTGT CCCCCTGCAG CAGTACAGGC AAGCTCAGCC GGTCAGACGA GCAGCTGTCC
    TCCCTAGACA GGGATAGCGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC
    TATGACCCCG ACTATGAATT CCTCCAGCAA GACCTCTCCA ATGCAGACCA GACCCCTCCA
    CAGGCAGCCT GTAACCTCAG CCCACTGCCG GAGTCCATGG GGGAGTCTGG GCCCCCATTT
    GTTGGCTACT CTTTCCAGCT GCCTCTGGGC AGCTGTCTCC AGCAGGATGG ACAGCAGACA
    GATACTCCAC CTGCCCTTCC TGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC
    AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAA
    GATGATCTGC AGAACCCAGC CCAGCCTGTG TCCTACCCGC CCTTTGCTGC TATCCTGCCC
    TTTCAGCAGG GAGCTTCTTC CACCCCTGTT GAGTTTGTGG GTGATTTCAG TGTTCCTGAG
    TCAGCCGGTG ACCCAGAAAA GCCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG
    GCCTACATGC AACTGCTGGA GGACTACTCA GAGCCACAGC CCTCCATGTT CTACCAGACA
    CCGCAGAGTG AGCACATCTA CCAGCAGAAG AACAAGATGC TCATGGAGGT CTATGGCTTC
    AGCGACTCCT TCTGTGGTAG TGACTCCACG CAGGAGCTGG CCCCTCCACC CGCTTTGCCC
    CCCAAGCAGC GGCAGCTGGC CTCCTATGCT GCTTCTTCCT TCTCTGTCTC CCACTGTGTC
    CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTA
    TCCGTGTCTA ACTCCTTTCT CAACCGGCAC GGCAGCTTGC CTGTGCCCTC GGAACCGCCA
    TCTGGGAAGG ACGGACATCC CAGAGATCTT TCGGTCAGCA GTGCATCTGG GAAGGACAGC
    AGAGAAAATG GGGAGAGGTC CCCGAAGTCA CTGGATGCTC TGGAGTCAGC CCAGTCAGAA
    GAGGAAGTGG ATGAACTGTC CCTCATTGAC CACAATGAAA TCATGGCCAG GCTGACACTC
    AAGCAAGAGG GTGATGATGG ACCAGATGTT CGTGGAGGTT CAGGAGACAT CTTACTGGTC
    CATGCTACTG AGACAGACAG AAAAGACCTG GTCCTGTACT GTGAAGCCTT CCTGACCACC
    TACAGAACCT TCATCAGCCC CGAGGAGCTC ATCAAGAAGC TGCAGTGCCG GTACGAGAAG
    TTCTCTCCCT TTGCTGACAC ATTCAAGAAG CGAGTGAGCA AGAACACATT CTTCGTGCTG
    GTGCGGGTGG TGGACGAGCT CTGCCTGGTG GAGCTGACAG AGGAGATCCT GAAGCTGCTG
    ATGGAGCTAG TCTTCCGCCT AGTGTGCAGT GGAGAGCTCA GCTTGGCCAG GGTACTCCGA
    AAGAACATTC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCCA TTCCGACCAG
    CCTTTGGCAG CCAGGGGTGT TGCAGCCAGG CCAGGAACCT TGCATGATTT CCACAGCCAC
    GAGATAGCTG AACAGCTGAC ACTGCTGGAT GCCGAGCTTT TCTATAAGAT AGAGATTCCC
    GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT GAAGAGAAGA GCCCCAATCT GACCCAGTTC
    ACAGAGCACT TCAACAACAT GTCCTACTGG GTGCGGTCCG TCATCATGCT GCAGGAGAAG
    GCCCAGGACC GGGAGAGGCT GCTCCTGAAG TTCATCAAGA TCATGAAGCA CCTGCGCAAG
    CTCAACAATT TCAACTCCTA CCTGGCCATC CTCTCCGCAC TGGACTCGGC CCCCATCCGC
    AGACTGGAGT GGCAGCGACA GACCTCAGAG GGCCTGGCTG AGTACTGCAC GTTGATTGAC
    AGCTCATCCT CCTTCCGAGC CTACCGGGCT GCCCTCTCAG AGGTGGAACC TCCATGTATC
    CCATACCTAG GTTTGATCCT GCAAGACCTG ACGTTCGTCC ACCTGGGAAA CCCAGACTAC
    ATAGACGGGA AGGTCAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT GGACAGCATG
    CGCTGTTTCC AGCAGGTGCA CTATGAAATC CGGAGAAACG ACGACATCAT AAACTTCTTC
    AATGACTTCA GCGACCACCT GGCCGAGGAA GCCCTATGGG AACTCTCTCT GAAGATCAAG
    CCTAGGAACA TAACAAGGCG GAAAACAGAC CGTGAAGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60575
    Clone ID Related Accession (Same CDS sequence) XM_017592109.1
    Accession Version XM_017592109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product rap guanine nucleotide exchange factor 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447598.1
    CDS3180..6461
    Translation

    Target ORF information:

    RefSeq Version XM_017592109.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus Rap guanine nucleotide exchange factor 1 (Rapgef1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CCATTGAAAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
    CAAGACTCTC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC
    TCTCCTAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCTGAGGT GTCGAAGACT
    CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC
    CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGATTCCAGG
    TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC
    TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG
    CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT
    CGGATCCAGC ACAGCTCAGC CCTCTCCTCT TGCTATAGCC GAGTGTACCA GAGCCTTGCC
    AACCTTATCC GCTGGTCTGA CCAGGTGATG CTCGAGGGTG TGAACTCAGA AGATAAGGAG
    ATGGTGACGA CTGTGAAGGG TGTCATCAAA GCTGTGCTGG ATGGAGTGAA GGAACTGGTG
    AGGCTAACCA TTGAGAAGCA AGGCCGGCCA TCACCAACAA GCCCAGTGAA GCCCAGTTCC
    CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGAACGAGA AATGGAGATT
    CTGAACAAGA CAAGTGTGTC ACCATCCACT GAACTGCTCC CAGACTCCAC CAGTGAAGAG
    GTTGCGCCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TTTGCCTGGA
    TTTTCTGTCT CTTCTAGTCC ACCAGCATTA CCACCCAAGA AAAGGCAATC TGCTCCATCC
    CCTACCCGGG TGGCTGTGGT AGCCCCCATG AGTCGTGCTG TCAGTGGCTC CAGTTTGCCT
    GTTGGAATCA ATAGGCAGGA CTTTGATGTT GAATGTTACA CCCAGAGGCG CCTGTCAGGA
    GGCAGCCGCT CCTGTGGTGG TGAATCTCCT CGCCTGTCCC CCTGCAGCAG TACAGGCAAG
    CTCAGCCGGT CAGACGAGCA GCTGTCCTCC CTAGACAGGG ATAGCGGGCA GTGCTCACGG
    AACACAAGCT GTGAAACACT AGATCACTAT GACCCCGACT ATGAATTCCT CCAGCAAGAC
    CTCTCCAATG CAGACCAGAC CCCTCCACAG GCAGCCTGTA ACCTCAGCCC ACTGCCGGAG
    TCCATGGGGG AGTCTGGGCC CCCATTTGTT GGCTACTCTT TCCAGCTGCC TCTGGGCAGC
    TGTCTCCAGC AGGATGGACA GCAGACAGAT ACTCCACCTG CCCTTCCTGA GAAGAAGCGT
    AGGAGCGCAG TCTCCCAGAC CACGGACAGC TCTGGCTGCA GGGTGTCCTA TGAGCGACAC
    CCCTCACAGT ATGACAACAT CTCAGAAGAT GATCTGCAGA ACCCAGCCCA GCCTGTGTCC
    TACCCGCCCT TTGCTGCTAT CCTGCCCTTT CAGCAGGGAG CTTCTTCCAC CCCTGTTGAG
    TTTGTGGGTG ATTTCAGTGT TCCTGAGTCA GCCGGTGACC CAGAAAAGCC ACCTCCTCTA
    CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAAC TGCTGGAGGA CTACTCAGAG
    CCACAGCCCT CCATGTTCTA CCAGACACCG CAGAGTGAGC ACATCTACCA GCAGAAGAAC
    AAGATGCTCA TGGAGGTCTA TGGCTTCAGC GACTCCTTCT GTGGTAGTGA CTCCACGCAG
    GAGCTGGCCC CTCCACCCGC TTTGCCCCCC AAGCAGCGGC AGCTGGAACC GCCATCTGGG
    AAGGACGGAC ATCCCAGAGA TCTTTCGGTC AGCAGTGCAT CTGGGAAGGA CAGCAGAGAA
    AATGGGGAGA GGTCCCCGAA GTCACTGGAT GCTCTGGAGT CAGCCCAGTC AGAAGAGGAA
    GTGGATGAAC TGTCCCTCAT TGACCACAAT GAAATCATGG CCAGGCTGAC ACTCAAGCAA
    GAGGGTGATG ATGGACCAGA TGTTCGTGGA GGTTCAGGAG ACATCTTACT GGTCCATGCT
    ACTGAGACAG ACAGAAAAGA CCTGGTCCTG TACTGTGAAG CCTTCCTGAC CACCTACAGA
    ACCTTCATCA GCCCCGAGGA GCTCATCAAG AAGCTGCAGT GCCGGTACGA GAAGTTCTCT
    CCCTTTGCTG ACACATTCAA GAAGCGAGTG AGCAAGAACA CATTCTTCGT GCTGGTGCGG
    GTGGTGGACG AGCTCTGCCT GGTGGAGCTG ACAGAGGAGA TCCTGAAGCT GCTGATGGAG
    CTAGTCTTCC GCCTAGTGTG CAGTGGAGAG CTCAGCTTGG CCAGGGTACT CCGAAAGAAC
    ATTCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCCATTCCGA CCAGCCTTTG
    GCAGCCAGGG GTGTTGCAGC CAGGCCAGGA ACCTTGCATG ATTTCCACAG CCACGAGATA
    GCTGAACAGC TGACACTGCT GGATGCCGAG CTTTTCTATA AGATAGAGAT TCCCGAAGTT
    TTGCTTTGGG CCAAAGAGCA GAATGAAGAG AAGAGCCCCA ATCTGACCCA GTTCACAGAG
    CACTTCAACA ACATGTCCTA CTGGGTGCGG TCCGTCATCA TGCTGCAGGA GAAGGCCCAG
    GACCGGGAGA GGCTGCTCCT GAAGTTCATC AAGATCATGA AGCACCTGCG CAAGCTCAAC
    AATTTCAACT CCTACCTGGC CATCCTCTCC GCACTGGACT CGGCCCCCAT CCGCAGACTG
    GAGTGGCAGC GACAGACCTC AGAGGGCCTG GCTGAGTACT GCACGTTGAT TGACAGCTCA
    TCCTCCTTCC GAGCCTACCG GGCTGCCCTC TCAGAGGTGG AACCTCCATG TATCCCATAC
    CTAGGTTTGA TCCTGCAAGA CCTGACGTTC GTCCACCTGG GAAACCCAGA CTACATAGAC
    GGGAAGGTCA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTGGACAG CATGCGCTGT
    TTCCAGCAGG TGCACTATGA AATCCGGAGA AACGACGACA TCATAAACTT CTTCAATGAC
    TTCAGCGACC ACCTGGCCGA GGAAGCCCTA TGGGAACTCT CTCTGAAGAT CAAGCCTAGG
    AACATAACAA GGCGGAAAAC AGACCGTGAA GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.