Rapgef1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rapgef1
Entrez Gene ID	107746
Full Name	Rap guanine nucleotide exchange factor (GEF) 1
Synonyms	4932418O06Rik,C3G,Grf2
General protein information	Preferred Names Rap guanine nucleotide exchange factor (GEF) 1 Names rap guanine nucleotide exchange factor 1 guanine nucleotide releasing factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 20.32 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497587.3	XP_006497650.1	rap guanine nucleotide exchange factor 1 isoform X6
NM_054050.2	NP_473391.1	rap guanine nucleotide exchange factor 1 isoform 3
NM_001039086.1	NP_001034175.1	rap guanine nucleotide exchange factor 1 isoform 2
XM_030246483.1	XP_030102343.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_006497582.3	XP_006497645.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_006497583.2	XP_006497646.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_030246484.1	XP_030102344.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_030246485.1	XP_030102345.1	rap guanine nucleotide exchange factor 1 isoform X13
NM_001039087.1	NP_001034176.1	rap guanine nucleotide exchange factor 1 isoform 1
XM_006497583.1	XP_006497646.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_006497585.3	XP_006497648.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_006497588.1	XP_006497651.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_006497586.3	XP_006497649.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_006497584.3	XP_006497647.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_006497585.4	XP_006497648.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_030246481.1	XP_030102341.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_006497586.4	XP_006497649.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_030246482.1	XP_030102342.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_006497587.4	XP_006497650.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_006497590.3	XP_006497653.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_030246487.1	XP_030102347.1	rap guanine nucleotide exchange factor 1 isoform X15
NM_001362702.1	NP_001349631.1	rap guanine nucleotide exchange factor 1 isoform 4
XM_006497589.3	XP_006497652.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_006497592.3	XP_006497655.1	rap guanine nucleotide exchange factor 1 isoform X14
XM_030246488.1	XP_030102348.1	rap guanine nucleotide exchange factor 1 isoform X16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Gene Ontology
Bibliography

Related articles in PubMed

C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development.
Shah B, Lutter D, Bochenek ML, Kato K, Tsytsyura Y, Glyvuk N, Sakakibara A, Klingauf J, Adams RH, P?schel AW
PloS one11(4)e0154174(2016)

C3G (RapGEF1), a regulator of actin dynamics promotes survival and myogenic differentiation of mouse mesenchymal cells.
Sasi Kumar K, Ramadhas A, Nayak SC, Kaniyappan S, Dayma K, Radha V
Biochimica et biophysica acta1853(10 Pt A)2629-39(2015 Oct)

Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis.
Maddala R, Nagendran T, Lang RA, Morozov A, Rao PV
Developmental biology406(1)74-91(2015 Oct)

Quantitative comparison of CrkL-SH3 binding proteins from embryonic murine brain and liver: Implications for developmental signaling and the quantification of protein species variants in bottom-up proteomics.
Cheerathodi M, Vincent JJ, Ballif BA
Journal of proteomics125104-11(2015 Jul)

CRK proteins selectively regulate T cell migration into inflamed tissues.
Huang Y, Clarke F, Karimi M, Roy NH, Williamson EK, Okumura M, Mochizuki K, Chen EJ, Park TJ, Debes GF, Zhang Y, Curran T, Kambayashi T, Burkhardt JK
The Journal of clinical investigation125(3)1019-32(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C3G is required in cortical neurons for both the specification of an axon and the initiation of radial migration by forming a leading process.
Title: C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development.

results provide evidence that C3G plays an important role in myogenic differentiation by coordinating cell cycle exit, actin dynamics and survival signaling.
Title: C3G (RapGEF1), a regulator of actin dynamics promotes survival and myogenic differentiation of mouse mesenchymal cells.

C3G regulates sympathetic preganglionic neuron migration by acting downstream in the reelin signaling pathway.
Title: Migration of sympathetic preganglionic neurons in the spinal cord of a C3G-deficient mouse suggests that C3G acts in the reelin signaling pathway.

Data indicate that chemical rescue of c-Src provided a tool to dissect the spatiotemporal mechanism of activation of the Rap1 guanine exchange factor, C3G, one of the identified potential c-Src substrates that plays a role in focal adhesion signaling.
Title: Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics.

GEF and Dlx1/2 form part of a common signalling pathway during GABAergic neuronal development.
Title: Expression of the Rap1 guanine nucleotide exchange factor, MR-GEF, is altered in individuals with bipolar disorder.

The following Rapgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29221	XM_006497583.2 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29223	XM_006497585.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29224	XM_006497586.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29225	XM_006497587.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87656	XM_030246481.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87657	XM_030246482.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87658	XM_030246483.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87659	XM_030246484.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87660	XM_030246485.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87661	XM_030246487.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87662	XM_030246488.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu00397	NM_001039086.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu00400	NM_001039087.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu09514	NM_054050.2 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu29226	NM_001362702.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29220	XM_006497582.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29222	XM_006497584.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29226	XM_006497588.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29227	XM_006497589.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu29228	XM_006497590.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu29230	XM_006497592.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu29221	XM_006497583.1	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29223	XM_006497585.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29224	XM_006497586.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29225	XM_006497587.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rapgef1 ( XM_006497583.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497646.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29221
Clone ID Related Accession (Same CDS sequence)	XM_006497583.2 , XM_006497583.1
Accession Version	XM_006497583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497646.1
CDS	284..4054
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497583.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497583.2

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497585.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497648.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.

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CloneID	OMu29223
Clone ID Related Accession (Same CDS sequence)	XM_006497585.3 , XM_006497585.4
Accession Version	XM_006497585.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497585.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497648.1
CDS	164..3841
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497585.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497585.4

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497586.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497649.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu29224
Clone ID Related Accession (Same CDS sequence)	XM_006497586.3 , XM_006497586.4
Accession Version	XM_006497586.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGCAGGCCT CCTATGCTGC TTCTTCCTTC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCC TCAGTGTATC CGTGTCTAAC TCCTTTCTCA ACCGGCACGG CAGCTTGCCT 
GTGCCCTCGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA 
GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC 
CACACGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC 
GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAAGGCCA GCAGTGAGGA AGCTGGTGGG 
GATGAGTATG TCAGTCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTGGC TCCCTGTCGA 
GGAGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497649.1
CDS	171..3830
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497586.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497586.4

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGCAGGCCT CCTATGCTGC TTCTTCCTTC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCC TCAGTGTATC CGTGTCTAAC TCCTTTCTCA ACCGGCACGG CAGCTTGCCT 
GTGCCCTCGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA 
GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC 
CACACGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC 
GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAAGGCCA GCAGTGAGGA AGCTGGTGGG 
GATGAGTATG TCAGTCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTGGC TCCCTGTCGA 
GGAGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497587.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497650.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X8, mRNA.

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CloneID	OMu29225
Clone ID Related Accession (Same CDS sequence)	XM_006497587.3 , XM_006497587.4
Accession Version	XM_006497587.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497587.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC 
TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CACGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC 
AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT 
GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAAGGCCAGC 
AGTGAGGAAG CTGGTGGGGA TGAGTATGTC AGTCTGTACT CCTCTGGCCA GACCAGCGAG 
GAGCTGGCTC CCTGTCGAGG AGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497650.1
CDS	165..3782
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497587.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497587.4

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC 
TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CACGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC 
AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT 
GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAAGGCCAGC 
AGTGAGGAAG CTGGTGGGGA TGAGTATGTC AGTCTGTACT CCTCTGGCCA GACCAGCGAG 
GAGCTGGCTC CCTGTCGAGG AGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246481.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102341.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu87656
Clone ID Related Accession (Same CDS sequence)	XM_030246481.1
Accession Version	XM_030246481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGGA ACCACCATCT GGGAAGGACG GACATCCCAG AGATCCTTCG 
GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AAAGGTCCCC AAAGTCACTG 
GATGGTCTGG AGTCAGCGCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC 
AATGAAATTA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGGCC AGATGTTCGT 
GGTGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTG 
CTGTACTGTG AAGCCTTCCT GACCACCTAC AGGACCTTCA TCAGCCCGGA GGAGCTCATC 
AAGAAGCTGC AGTACCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACGTT CAAGAAGCGA 
GTGAGCAAGA ACACATTCTT TGTGCTGGTT CGAGTGGTGG ATGAGCTCTG CCTGGTGGAG 
CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTAGT GTGCAGCGGA 
GAGCTCAGCC TGGCCAGAGT CCTCCGGAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCCATTC CGACCAGCCT CTGGCAGCCA GGGGTGTTGC AGCCAGGCCA 
GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAGC AGCTGACACT GCTGGATGCC 
GAGCTTTTCT ACAAGATAGA GATTCCTGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAG 
GAGAAGAGTC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG 
CGGTCCATCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTCAAGTTC 
ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAACTTCA ACTCCTACCT GGCCATCCTC 
TCAGCACTAG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGGCAGAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACATT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCT 
CTCTCAGAGG TGGAGCCCCC ATGTATCCCA TACCTAGGTC TGATTCTGCA AGACCTGACC 
TTCGTTCACC TGGGAAACCC AGATTATATT GACGGGAAAG TAAACTTCTC CAAGCGGTGG 
CAACAGTTCA ACATATTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGAAATCCGG 
AGAAACGACG ACATCATAAA TTTCTTCAAT GACTTCAGTG ACCACCTGGC CGAGGAAGCC 
CTGTGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGC 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102341.1
CDS	164..3838
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246481.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246481.1

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGGA ACCACCATCT GGGAAGGACG GACATCCCAG AGATCCTTCG 
GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AAAGGTCCCC AAAGTCACTG 
GATGGTCTGG AGTCAGCGCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC 
AATGAAATTA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGGCC AGATGTTCGT 
GGTGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTG 
CTGTACTGTG AAGCCTTCCT GACCACCTAC AGGACCTTCA TCAGCCCGGA GGAGCTCATC 
AAGAAGCTGC AGTACCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACGTT CAAGAAGCGA 
GTGAGCAAGA ACACATTCTT TGTGCTGGTT CGAGTGGTGG ATGAGCTCTG CCTGGTGGAG 
CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTAGT GTGCAGCGGA 
GAGCTCAGCC TGGCCAGAGT CCTCCGGAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCCATTC CGACCAGCCT CTGGCAGCCA GGGGTGTTGC AGCCAGGCCA 
GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAGC AGCTGACACT GCTGGATGCC 
GAGCTTTTCT ACAAGATAGA GATTCCTGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAG 
GAGAAGAGTC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG 
CGGTCCATCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTCAAGTTC 
ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAACTTCA ACTCCTACCT GGCCATCCTC 
TCAGCACTAG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGGCAGAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACATT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCT 
CTCTCAGAGG TGGAGCCCCC ATGTATCCCA TACCTAGGTC TGATTCTGCA AGACCTGACC 
TTCGTTCACC TGGGAAACCC AGATTATATT GACGGGAAAG TAAACTTCTC CAAGCGGTGG 
CAACAGTTCA ACATATTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGAAATCCGG 
AGAAACGACG ACATCATAAA TTTCTTCAAT GACTTCAGTG ACCACCTGGC CGAGGAAGCC 
CTGTGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGC 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246482.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102342.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu87657
Clone ID Related Accession (Same CDS sequence)	XM_030246482.1
Accession Version	XM_030246482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAG GCCAGCAGTG AGGAAGCTGG TGGGGATGAG 
TATGTCAGTC TGTACTCCTC TGGCCAGACC AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA 
CCACCATCTG GGAAGGACGG ACATCCCAGA GATCCTTCGG TCAGCAGTGC ATCTGGGAAG 
GACAGCAGAG AAAATGGGGA AAGGTCCCCA AAGTCACTGG ATGGTCTGGA GTCAGCGCAG 
TCAGAAGAGG AAGTGGATGA ACTGTCCCTC ATTGACCACA ATGAAATTAT GGCCAGGCTG 
ACACTCAAGC AAGAGGGTGA TGATGGGCCA GATGTTCGTG GTGGTTCAGG AGACATCTTA 
CTGGTCCATG CTACTGAGAC AGACAGAAAA GACCTGGTGC TGTACTGTGA AGCCTTCCTG 
ACCACCTACA GGACCTTCAT CAGCCCGGAG GAGCTCATCA AGAAGCTGCA GTACCGGTAC 
GAGAAGTTCT CTCCCTTTGC TGACACGTTC AAGAAGCGAG TGAGCAAGAA CACATTCTTT 
GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC CTGGTGGAGC TGACAGAGGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTAGTG TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC 
CTCCGGAAGA ACATTCTGGA CAAGGTGGAC CAGAAGAAGC TGCTCAGGTG TGCCCATTCC 
GACCAGCCTC TGGCAGCCAG GGGTGTTGCA GCCAGGCCAG GAACCTTGCA TGATTTCCAC 
AGCCACGAGA TAGCTGAGCA GCTGACACTG CTGGATGCCG AGCTTTTCTA CAAGATAGAG 
ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG CAGAATGAGG AGAAGAGTCC CAATCTGACC 
CAGTTCACAG AGCACTTCAA CAACATGTCC TACTGGGTGC GGTCCATCAT CATGCTGCAG 
GAGAAGGCCC AGGACCGGGA GAGGCTGCTC CTCAAGTTCA TCAAGATCAT GAAGCACCTG 
CGCAAGCTCA ACAACTTCAA CTCCTACCTG GCCATCCTCT CAGCACTAGA CTCGGCCCCC 
ATCCGCAGAC TGGAGTGGCA GCGGCAGACC TCAGAGGGCC TGGCTGAGTA CTGCACATTG 
ATTGACAGCT CATCCTCCTT CCGAGCCTAC CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA 
TGTATCCCAT ACCTAGGTCT GATTCTGCAA GACCTGACCT TCGTTCACCT GGGAAACCCA 
GATTATATTG ACGGGAAAGT AAACTTCTCC AAGCGGTGGC AACAGTTCAA CATATTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAT GAAATCCGGA GAAACGACGA CATCATAAAT 
TTCTTCAATG ACTTCAGTGA CCACCTGGCC GAGGAAGCCC TGTGGGAACT CTCTCTGAAG 
ATCAAGCCTA GGAACATAAC AAGGCGGAAA ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102342.1
CDS	284..3937
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246482.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246482.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAG GCCAGCAGTG AGGAAGCTGG TGGGGATGAG 
TATGTCAGTC TGTACTCCTC TGGCCAGACC AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA 
CCACCATCTG GGAAGGACGG ACATCCCAGA GATCCTTCGG TCAGCAGTGC ATCTGGGAAG 
GACAGCAGAG AAAATGGGGA AAGGTCCCCA AAGTCACTGG ATGGTCTGGA GTCAGCGCAG 
TCAGAAGAGG AAGTGGATGA ACTGTCCCTC ATTGACCACA ATGAAATTAT GGCCAGGCTG 
ACACTCAAGC AAGAGGGTGA TGATGGGCCA GATGTTCGTG GTGGTTCAGG AGACATCTTA 
CTGGTCCATG CTACTGAGAC AGACAGAAAA GACCTGGTGC TGTACTGTGA AGCCTTCCTG 
ACCACCTACA GGACCTTCAT CAGCCCGGAG GAGCTCATCA AGAAGCTGCA GTACCGGTAC 
GAGAAGTTCT CTCCCTTTGC TGACACGTTC AAGAAGCGAG TGAGCAAGAA CACATTCTTT 
GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC CTGGTGGAGC TGACAGAGGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTAGTG TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC 
CTCCGGAAGA ACATTCTGGA CAAGGTGGAC CAGAAGAAGC TGCTCAGGTG TGCCCATTCC 
GACCAGCCTC TGGCAGCCAG GGGTGTTGCA GCCAGGCCAG GAACCTTGCA TGATTTCCAC 
AGCCACGAGA TAGCTGAGCA GCTGACACTG CTGGATGCCG AGCTTTTCTA CAAGATAGAG 
ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG CAGAATGAGG AGAAGAGTCC CAATCTGACC 
CAGTTCACAG AGCACTTCAA CAACATGTCC TACTGGGTGC GGTCCATCAT CATGCTGCAG 
GAGAAGGCCC AGGACCGGGA GAGGCTGCTC CTCAAGTTCA TCAAGATCAT GAAGCACCTG 
CGCAAGCTCA ACAACTTCAA CTCCTACCTG GCCATCCTCT CAGCACTAGA CTCGGCCCCC 
ATCCGCAGAC TGGAGTGGCA GCGGCAGACC TCAGAGGGCC TGGCTGAGTA CTGCACATTG 
ATTGACAGCT CATCCTCCTT CCGAGCCTAC CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA 
TGTATCCCAT ACCTAGGTCT GATTCTGCAA GACCTGACCT TCGTTCACCT GGGAAACCCA 
GATTATATTG ACGGGAAAGT AAACTTCTCC AAGCGGTGGC AACAGTTCAA CATATTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAT GAAATCCGGA GAAACGACGA CATCATAAAT 
TTCTTCAATG ACTTCAGTGA CCACCTGGCC GAGGAAGCCC TGTGGGAACT CTCTCTGAAG 
ATCAAGCCTA GGAACATAAC AAGGCGGAAA ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246483.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102343.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X9, mRNA.

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CloneID	OMu87658
Clone ID Related Accession (Same CDS sequence)	XM_030246483.1
Accession Version	XM_030246483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGGCCTCCT ATGCTGCTTC TTCCTTCTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTATCCGT GTCTAACTCC TTTCTCAACC GGCACGGCAG CTTGCCTGTG 
CCCTCGTACA AATCTGTGTT TAGGTCTTAC TCCCAGGACT TCATGCCTCA CCACCAAGCC 
TCCGTCCAAC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGTCCAC 
ACGTCCCAGA GCTCGGACTT AGCGGTGCCC ACCGTAAGCA GTCCGCCTCC CAGCACCGTG 
GACGGGCCTC TCTCGTCTTC TCAGGACAGC AGCTTTCATG GGAACCCTGT CCGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAACCA CCATCTGGGA AGGACGGACA TCCCAGAGAT 
CCTTCGGTCA GCAGTGCATC TGGGAAGGAC AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG 
TCACTGGATG GTCTGGAGTC AGCGCAGTCA GAAGAGGAAG TGGATGAACT GTCCCTCATT 
GACCACAATG AAATTATGGC CAGGCTGACA CTCAAGCAAG AGGGTGATGA TGGGCCAGAT 
GTTCGTGGTG GTTCAGGAGA CATCTTACTG GTCCATGCTA CTGAGACAGA CAGAAAAGAC 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACC ACCTACAGGA CCTTCATCAG CCCGGAGGAG 
CTCATCAAGA AGCTGCAGTA CCGGTACGAG AAGTTCTCTC CCTTTGCTGA CACGTTCAAG 
AAGCGAGTGA GCAAGAACAC ATTCTTTGTG CTGGTTCGAG TGGTGGATGA GCTCTGCCTG 
GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTAGTGTGC 
AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC CGGAAGAACA TTCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CCATTCCGAC CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC 
AGGCCAGGAA CCTTGCATGA TTTCCACAGC CACGAGATAG CTGAGCAGCT GACACTGCTG 
GATGCCGAGC TTTTCTACAA GATAGAGATT CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG 
AATGAGGAGA AGAGTCCCAA TCTGACCCAG TTCACAGAGC ACTTCAACAA CATGTCCTAC 
TGGGTGCGGT CCATCATCAT GCTGCAGGAG AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC 
AAGTTCATCA AGATCATGAA GCACCTGCGC AAGCTCAACA ACTTCAACTC CTACCTGGCC 
ATCCTCTCAG CACTAGACTC GGCCCCCATC CGCAGACTGG AGTGGCAGCG GCAGACCTCA 
GAGGGCCTGG CTGAGTACTG CACATTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCTGCTCTCT CAGAGGTGGA GCCCCCATGT ATCCCATACC TAGGTCTGAT TCTGCAAGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAT TATATTGACG GGAAAGTAAA CTTCTCCAAG 
CGGTGGCAAC AGTTCAACAT ATTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAA 
ATCCGGAGAA ACGACGACAT CATAAATTTC TTCAATGACT TCAGTGACCA CCTGGCCGAG 
GAAGCCCTGT GGGAACTCTC TCTGAAGATC AAGCCTAGGA ACATAACAAG GCGGAAAACA 
GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102343.1
CDS	171..3731
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246483.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246483.1

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGGCCTCCT ATGCTGCTTC TTCCTTCTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTATCCGT GTCTAACTCC TTTCTCAACC GGCACGGCAG CTTGCCTGTG 
CCCTCGTACA AATCTGTGTT TAGGTCTTAC TCCCAGGACT TCATGCCTCA CCACCAAGCC 
TCCGTCCAAC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGTCCAC 
ACGTCCCAGA GCTCGGACTT AGCGGTGCCC ACCGTAAGCA GTCCGCCTCC CAGCACCGTG 
GACGGGCCTC TCTCGTCTTC TCAGGACAGC AGCTTTCATG GGAACCCTGT CCGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAACCA CCATCTGGGA AGGACGGACA TCCCAGAGAT 
CCTTCGGTCA GCAGTGCATC TGGGAAGGAC AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG 
TCACTGGATG GTCTGGAGTC AGCGCAGTCA GAAGAGGAAG TGGATGAACT GTCCCTCATT 
GACCACAATG AAATTATGGC CAGGCTGACA CTCAAGCAAG AGGGTGATGA TGGGCCAGAT 
GTTCGTGGTG GTTCAGGAGA CATCTTACTG GTCCATGCTA CTGAGACAGA CAGAAAAGAC 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACC ACCTACAGGA CCTTCATCAG CCCGGAGGAG 
CTCATCAAGA AGCTGCAGTA CCGGTACGAG AAGTTCTCTC CCTTTGCTGA CACGTTCAAG 
AAGCGAGTGA GCAAGAACAC ATTCTTTGTG CTGGTTCGAG TGGTGGATGA GCTCTGCCTG 
GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTAGTGTGC 
AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC CGGAAGAACA TTCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CCATTCCGAC CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC 
AGGCCAGGAA CCTTGCATGA TTTCCACAGC CACGAGATAG CTGAGCAGCT GACACTGCTG 
GATGCCGAGC TTTTCTACAA GATAGAGATT CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG 
AATGAGGAGA AGAGTCCCAA TCTGACCCAG TTCACAGAGC ACTTCAACAA CATGTCCTAC 
TGGGTGCGGT CCATCATCAT GCTGCAGGAG AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC 
AAGTTCATCA AGATCATGAA GCACCTGCGC AAGCTCAACA ACTTCAACTC CTACCTGGCC 
ATCCTCTCAG CACTAGACTC GGCCCCCATC CGCAGACTGG AGTGGCAGCG GCAGACCTCA 
GAGGGCCTGG CTGAGTACTG CACATTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCTGCTCTCT CAGAGGTGGA GCCCCCATGT ATCCCATACC TAGGTCTGAT TCTGCAAGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAT TATATTGACG GGAAAGTAAA CTTCTCCAAG 
CGGTGGCAAC AGTTCAACAT ATTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAA 
ATCCGGAGAA ACGACGACAT CATAAATTTC TTCAATGACT TCAGTGACCA CCTGGCCGAG 
GAAGCCCTGT GGGAACTCTC TCTGAAGATC AAGCCTAGGA ACATAACAAG GCGGAAAACA 
GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246484.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102344.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu87659
Clone ID Related Accession (Same CDS sequence)	XM_030246484.1
Accession Version	XM_030246484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102344.1
CDS	284..3700
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246484.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246484.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246485.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102345.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X13, mRNA.

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CloneID	OMu87660
Clone ID Related Accession (Same CDS sequence)	XM_030246485.1
Accession Version	XM_030246485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG 
GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCC 
TGTTATAGCC GAGTATACCA GAGCCTCGCC AACCTTATCC GATGGTCTGA CCAGGTGATG 
CTAGAGGGCG TGAACTCAGA AGATAAGGAG ATGGTGACAA CTGTGAAGGG CGTTATCAAA 
GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG AGGCTAACCA TTGAGAAGCA GGGGCGGCCA 
TCGCCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG 
CTCCCTCTGA CAGACCGAGA AATGGAGATT CTGAACAAGA CGACAAGTGT GTCACCATCT 
GCTGAACTGC TCCCAGACTC CACCAGTGAA GAGGTCGCAC CCCCCAAGCC CCCTTTACCT 
GGCATCCGGG TGGTTGATAA CAGTCCACCA GCATTACCAC CCAAGAAAAG GCAGTCTGCT 
CCATCCCCCA CTCGGGTGGC TGTGGTAGCC CCAATGAGTC GGGCTACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG 
TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG TCTCCTCGCC TGTCCCCCTG CAGCAGCACA 
GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG TCCTCCCTGG ACAGGGATAG TGGGCAGTGC 
TCACGGAACA CAAGCTGTGA AACACTAGAT CACTACGACC CCGACTATGA ATTCCTCCAG 
CAAGATCTCT CCAATGCAGA CCAGATCCCT CCACAGGCAG CCTGTAACCT CAGCCCTCTG 
CCGGAGTCCC TGGGGGAATC TGGGCCTCCA TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG 
GGCAGCTGTC TGCAGCAGGA GGGACAGCAG ACAGACACTC CACCTGCCCT TCCGGAGAAG 
AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG 
CGACACCCCT CACAGTATGA CAACATCTCA GAGGGTGACC TGCAGAACCC AGTCCCAGTC 
CAGCCTGTGC CCTACCCACC CTTTGCTGCT GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT 
GCCTCTGCTG AGTTTGTGGG TGATTTCAGT GTTCCTGAGT TGGCGGGTGA CACAGAGAAG 
CCACCTCCTC TACCAGAGAA GAAGAACAAG CACATGCTGG CCTACATGCA ACTGCTGGAG 
GACTACTCAG AGCCACAGCC CTCCATGTTC TACCAGACAC CGCAGAGTGA GCACATCTAC 
CAGCAGAAGA ACAAGATGCT CATGGAGGTG TACGGCTTCA GCGAGTCCTT CTGCGGTAGT 
GATTCCACGC AGGAGCTGGC CCCTCCACCC GCTCTGCCCC CCAAGCAACG GCAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TATCCGTGTC TAACTCCTTT 
CTCAACCGGC ACGGCAGCTT GCCTGTGCCC TCGTACAAAT CTGTGTTTAG GTCTTACTCC 
CAGGACTTCA TGCCTCACCA CCAAGCCTCC GTCCAACCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGTCCACACG TCCCAGAGCT CGGACTTAGC GGTGCCCACC 
GTAAGCAGTC CGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGACAGCAGC 
TTTCATGGGA ACCCTGTCCG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAG 
GCCAGCAGTG AGGAAGCTGG TGGGGATGAG TATGTCAGTC TGTACTCCTC TGGCCAGACC 
AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA CCACCATCTG GGAAGGACGG ACATCCCAGA 
GATCCTTCGG TCAGCAGTGC ATCTGGGAAG GACAGCAGAG AAAATGGGGA AAGGTCCCCA 
AAGTCACTGG ATGGTCTGGA GTCAGCGCAG TCAGAAGAGG AAGTGGATGA ACTGTCCCTC 
ATTGACCACA ATGAAATTAT GGCCAGGCTG ACACTCAAGC AAGAGGGTGA TGATGGGCCA 
GATGTTCGTG GTGGTTCAGG AGACATCTTA CTGGTCCATG CTACTGAGAC AGACAGAAAA 
GACCTGGTGC TGTACTGTGA AGCCTTCCTG ACCACCTACA GGACCTTCAT CAGCCCGGAG 
GAGCTCATCA AGAAGCTGCA GTACCGGTAC GAGAAGTTCT CTCCCTTTGC TGACACGTTC 
AAGAAGCGAG TGAGCAAGAA CACATTCTTT GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC 
CTGGTGGAGC TGACAGAGGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTAGTG 
TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC CTCCGGAAGA ACATTCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCCATTCC GACCAGCCTC TGGCAGCCAG GGGTGTTGCA 
GCCAGGCCAG GAACCTTGCA TGATTTCCAC AGCCACGAGA TAGCTGAGCA GCTGACACTG 
CTGGATGCCG AGCTTTTCTA CAAGATAGAG ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG 
CAGAATGAGG AGAAGAGTCC CAATCTGACC CAGTTCACAG AGCACTTCAA CAACATGTCC 
TACTGGGTGC GGTCCATCAT CATGCTGCAG GAGAAGGCCC AGGACCGGGA GAGGCTGCTC 
CTCAAGTTCA TCAAGATCAT GAAGCACCTG CGCAAGCTCA ACAACTTCAA CTCCTACCTG 
GCCATCCTCT CAGCACTAGA CTCGGCCCCC ATCCGCAGAC TGGAGTGGCA GCGGCAGACC 
TCAGAGGGCC TGGCTGAGTA CTGCACATTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA TGTATCCCAT ACCTAGGTCT GATTCTGCAA 
GACCTGACCT TCGTTCACCT GGGAAACCCA GATTATATTG ACGGGAAAGT AAACTTCTCC 
AAGCGGTGGC AACAGTTCAA CATATTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GAAATCCGGA GAAACGACGA CATCATAAAT TTCTTCAATG ACTTCAGTGA CCACCTGGCC 
GAGGAAGCCC TGTGGGAACT CTCTCTGAAG ATCAAGCCTA GGAACATAAC AAGGCGGAAA 
ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102345.1
CDS	344..3727
Translation	MAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246485.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246485.1

ORF Insert Sequence:

ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG 
GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCC 
TGTTATAGCC GAGTATACCA GAGCCTCGCC AACCTTATCC GATGGTCTGA CCAGGTGATG 
CTAGAGGGCG TGAACTCAGA AGATAAGGAG ATGGTGACAA CTGTGAAGGG CGTTATCAAA 
GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG AGGCTAACCA TTGAGAAGCA GGGGCGGCCA 
TCGCCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG 
CTCCCTCTGA CAGACCGAGA AATGGAGATT CTGAACAAGA CGACAAGTGT GTCACCATCT 
GCTGAACTGC TCCCAGACTC CACCAGTGAA GAGGTCGCAC CCCCCAAGCC CCCTTTACCT 
GGCATCCGGG TGGTTGATAA CAGTCCACCA GCATTACCAC CCAAGAAAAG GCAGTCTGCT 
CCATCCCCCA CTCGGGTGGC TGTGGTAGCC CCAATGAGTC GGGCTACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG 
TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG TCTCCTCGCC TGTCCCCCTG CAGCAGCACA 
GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG TCCTCCCTGG ACAGGGATAG TGGGCAGTGC 
TCACGGAACA CAAGCTGTGA AACACTAGAT CACTACGACC CCGACTATGA ATTCCTCCAG 
CAAGATCTCT CCAATGCAGA CCAGATCCCT CCACAGGCAG CCTGTAACCT CAGCCCTCTG 
CCGGAGTCCC TGGGGGAATC TGGGCCTCCA TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG 
GGCAGCTGTC TGCAGCAGGA GGGACAGCAG ACAGACACTC CACCTGCCCT TCCGGAGAAG 
AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG 
CGACACCCCT CACAGTATGA CAACATCTCA GAGGGTGACC TGCAGAACCC AGTCCCAGTC 
CAGCCTGTGC CCTACCCACC CTTTGCTGCT GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT 
GCCTCTGCTG AGTTTGTGGG TGATTTCAGT GTTCCTGAGT TGGCGGGTGA CACAGAGAAG 
CCACCTCCTC TACCAGAGAA GAAGAACAAG CACATGCTGG CCTACATGCA ACTGCTGGAG 
GACTACTCAG AGCCACAGCC CTCCATGTTC TACCAGACAC CGCAGAGTGA GCACATCTAC 
CAGCAGAAGA ACAAGATGCT CATGGAGGTG TACGGCTTCA GCGAGTCCTT CTGCGGTAGT 
GATTCCACGC AGGAGCTGGC CCCTCCACCC GCTCTGCCCC CCAAGCAACG GCAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TATCCGTGTC TAACTCCTTT 
CTCAACCGGC ACGGCAGCTT GCCTGTGCCC TCGTACAAAT CTGTGTTTAG GTCTTACTCC 
CAGGACTTCA TGCCTCACCA CCAAGCCTCC GTCCAACCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGTCCACACG TCCCAGAGCT CGGACTTAGC GGTGCCCACC 
GTAAGCAGTC CGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGACAGCAGC 
TTTCATGGGA ACCCTGTCCG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAG 
GCCAGCAGTG AGGAAGCTGG TGGGGATGAG TATGTCAGTC TGTACTCCTC TGGCCAGACC 
AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA CCACCATCTG GGAAGGACGG ACATCCCAGA 
GATCCTTCGG TCAGCAGTGC ATCTGGGAAG GACAGCAGAG AAAATGGGGA AAGGTCCCCA 
AAGTCACTGG ATGGTCTGGA GTCAGCGCAG TCAGAAGAGG AAGTGGATGA ACTGTCCCTC 
ATTGACCACA ATGAAATTAT GGCCAGGCTG ACACTCAAGC AAGAGGGTGA TGATGGGCCA 
GATGTTCGTG GTGGTTCAGG AGACATCTTA CTGGTCCATG CTACTGAGAC AGACAGAAAA 
GACCTGGTGC TGTACTGTGA AGCCTTCCTG ACCACCTACA GGACCTTCAT CAGCCCGGAG 
GAGCTCATCA AGAAGCTGCA GTACCGGTAC GAGAAGTTCT CTCCCTTTGC TGACACGTTC 
AAGAAGCGAG TGAGCAAGAA CACATTCTTT GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC 
CTGGTGGAGC TGACAGAGGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTAGTG 
TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC CTCCGGAAGA ACATTCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCCATTCC GACCAGCCTC TGGCAGCCAG GGGTGTTGCA 
GCCAGGCCAG GAACCTTGCA TGATTTCCAC AGCCACGAGA TAGCTGAGCA GCTGACACTG 
CTGGATGCCG AGCTTTTCTA CAAGATAGAG ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG 
CAGAATGAGG AGAAGAGTCC CAATCTGACC CAGTTCACAG AGCACTTCAA CAACATGTCC 
TACTGGGTGC GGTCCATCAT CATGCTGCAG GAGAAGGCCC AGGACCGGGA GAGGCTGCTC 
CTCAAGTTCA TCAAGATCAT GAAGCACCTG CGCAAGCTCA ACAACTTCAA CTCCTACCTG 
GCCATCCTCT CAGCACTAGA CTCGGCCCCC ATCCGCAGAC TGGAGTGGCA GCGGCAGACC 
TCAGAGGGCC TGGCTGAGTA CTGCACATTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA TGTATCCCAT ACCTAGGTCT GATTCTGCAA 
GACCTGACCT TCGTTCACCT GGGAAACCCA GATTATATTG ACGGGAAAGT AAACTTCTCC 
AAGCGGTGGC AACAGTTCAA CATATTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GAAATCCGGA GAAACGACGA CATCATAAAT TTCTTCAATG ACTTCAGTGA CCACCTGGCC 
GAGGAAGCCC TGTGGGAACT CTCTCTGAAG ATCAAGCCTA GGAACATAAC AAGGCGGAAA 
ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246487.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102347.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu87661
Clone ID Related Accession (Same CDS sequence)	XM_030246487.1
Accession Version	XM_030246487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GAACCACCAT CTGGGAAGGA CGGACATCCC 
AGAGATCCTT CGGTCAGCAG TGCATCTGGG AAGGACAGCA GAGAAAATGG GGAAAGGTCC 
CCAAAGTCAC TGGATGGTCT GGAGTCAGCG CAGTCAGAAG AGGAAGTGGA TGAACTGTCC 
CTCATTGACC ACAATGAAAT TATGGCCAGG CTGACACTCA AGCAAGAGGG TGATGATGGG 
CCAGATGTTC GTGGTGGTTC AGGAGACATC TTACTGGTCC ATGCTACTGA GACAGACAGA 
AAAGACCTGG TGCTGTACTG TGAAGCCTTC CTGACCACCT ACAGGACCTT CATCAGCCCG 
GAGGAGCTCA TCAAGAAGCT GCAGTACCGG TACGAGAAGT TCTCTCCCTT TGCTGACACG 
TTCAAGAAGC GAGTGAGCAA GAACACATTC TTTGTGCTGG TTCGAGTGGT GGATGAGCTC 
TGCCTGGTGG AGCTGACAGA GGAGATCCTG AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA 
GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA GTCCTCCGGA AGAACATTCT GGACAAGGTG 
GACCAGAAGA AGCTGCTCAG GTGTGCCCAT TCCGACCAGC CTCTGGCAGC CAGGGGTGTT 
GCAGCCAGGC CAGGAACCTT GCATGATTTC CACAGCCACG AGATAGCTGA GCAGCTGACA 
CTGCTGGATG CCGAGCTTTT CTACAAGATA GAGATTCCTG AAGTTTTGCT TTGGGCCAAA 
GAGCAGAATG AGGAGAAGAG TCCCAATCTG ACCCAGTTCA CAGAGCACTT CAACAACATG 
TCCTACTGGG TGCGGTCCAT CATCATGCTG CAGGAGAAGG CCCAGGACCG GGAGAGGCTG 
CTCCTCAAGT TCATCAAGAT CATGAAGCAC CTGCGCAAGC TCAACAACTT CAACTCCTAC 
CTGGCCATCC TCTCAGCACT AGACTCGGCC CCCATCCGCA GACTGGAGTG GCAGCGGCAG 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA TTGATTGACA GCTCATCCTC CTTCCGAGCC 
TACCGGGCTG CTCTCTCAGA GGTGGAGCCC CCATGTATCC CATACCTAGG TCTGATTCTG 
CAAGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGATTATA TTGACGGGAA AGTAAACTTC 
TCCAAGCGGT GGCAACAGTT CAACATATTG GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TATGAAATCC GGAGAAACGA CGACATCATA AATTTCTTCA ATGACTTCAG TGACCACCTG 
GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG AAGATCAAGC CTAGGAACAT AACAAGGCGG 
AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102347.1
CDS	284..3430
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246487.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246487.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GAACCACCAT CTGGGAAGGA CGGACATCCC 
AGAGATCCTT CGGTCAGCAG TGCATCTGGG AAGGACAGCA GAGAAAATGG GGAAAGGTCC 
CCAAAGTCAC TGGATGGTCT GGAGTCAGCG CAGTCAGAAG AGGAAGTGGA TGAACTGTCC 
CTCATTGACC ACAATGAAAT TATGGCCAGG CTGACACTCA AGCAAGAGGG TGATGATGGG 
CCAGATGTTC GTGGTGGTTC AGGAGACATC TTACTGGTCC ATGCTACTGA GACAGACAGA 
AAAGACCTGG TGCTGTACTG TGAAGCCTTC CTGACCACCT ACAGGACCTT CATCAGCCCG 
GAGGAGCTCA TCAAGAAGCT GCAGTACCGG TACGAGAAGT TCTCTCCCTT TGCTGACACG 
TTCAAGAAGC GAGTGAGCAA GAACACATTC TTTGTGCTGG TTCGAGTGGT GGATGAGCTC 
TGCCTGGTGG AGCTGACAGA GGAGATCCTG AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA 
GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA GTCCTCCGGA AGAACATTCT GGACAAGGTG 
GACCAGAAGA AGCTGCTCAG GTGTGCCCAT TCCGACCAGC CTCTGGCAGC CAGGGGTGTT 
GCAGCCAGGC CAGGAACCTT GCATGATTTC CACAGCCACG AGATAGCTGA GCAGCTGACA 
CTGCTGGATG CCGAGCTTTT CTACAAGATA GAGATTCCTG AAGTTTTGCT TTGGGCCAAA 
GAGCAGAATG AGGAGAAGAG TCCCAATCTG ACCCAGTTCA CAGAGCACTT CAACAACATG 
TCCTACTGGG TGCGGTCCAT CATCATGCTG CAGGAGAAGG CCCAGGACCG GGAGAGGCTG 
CTCCTCAAGT TCATCAAGAT CATGAAGCAC CTGCGCAAGC TCAACAACTT CAACTCCTAC 
CTGGCCATCC TCTCAGCACT AGACTCGGCC CCCATCCGCA GACTGGAGTG GCAGCGGCAG 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA TTGATTGACA GCTCATCCTC CTTCCGAGCC 
TACCGGGCTG CTCTCTCAGA GGTGGAGCCC CCATGTATCC CATACCTAGG TCTGATTCTG 
CAAGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGATTATA TTGACGGGAA AGTAAACTTC 
TCCAAGCGGT GGCAACAGTT CAACATATTG GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TATGAAATCC GGAGAAACGA CGACATCATA AATTTCTTCA ATGACTTCAG TGACCACCTG 
GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG AAGATCAAGC CTAGGAACAT AACAAGGCGG 
AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_030246488.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102348.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu87662
Clone ID Related Accession (Same CDS sequence)	XM_030246488.1
Accession Version	XM_030246488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG 
GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCC 
TGTTATAGCC GAGTATACCA GAGCCTCGCC AACCTTATCC GATGGTCTGA CCAGGTGATG 
CTAGAGGGCG TGAACTCAGA AGATAAGGAG ATGGTGACAA CTGTGAAGGG CGTTATCAAA 
GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG AGGCTAACCA TTGAGAAGCA GGGGCGGCCA 
TCGCCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG 
CTCCCTCTGA CAGACCGAGA AATGGAGATT CTGAACAAGA CGACAAGTGT GTCACCATCT 
GCTGAACTGC TCCCAGACTC CACCAGTGAA GAGGTCGCAC CCCCCAAGCC CCCTTTACCT 
GGCATCCGGG TGGTTGATAA CAGTCCACCA GCATTACCAC CCAAGAAAAG GCAGTCTGCT 
CCATCCCCCA CTCGGGTGGC TGTGGTAGCC CCAATGAGTC GGGCTACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG 
TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG TCTCCTCGCC TGTCCCCCTG CAGCAGCACA 
GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG TCCTCCCTGG ACAGGGATAG TGGGCAGTGC 
TCACGGAACA CAAGCTGTGA AACACTAGAT CACTACGACC CCGACTATGA ATTCCTCCAG 
CAAGATCTCT CCAATGCAGA CCAGATCCCT CCACAGGCAG CCTGTAACCT CAGCCCTCTG 
CCGGAGTCCC TGGGGGAATC TGGGCCTCCA TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG 
GGCAGCTGTC TGCAGCAGGA GGGACAGCAG ACAGACACTC CACCTGCCCT TCCGGAGAAG 
AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG 
CGACACCCCT CACAGTATGA CAACATCTCA GAGGGTGACC TGCAGAACCC AGTCCCAGTC 
CAGCCTGTGC CCTACCCACC CTTTGCTGCT GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT 
GCCTCTGCTG AGTTTGTGGG TGATTTCAGT GTTCCTGAGT TGGCGGGTGA CACAGAGAAG 
CCACCTCCTC TACCAGAGAA GAAGAACAAG CACATGCTGG CCTACATGCA ACTGCTGGAG 
GACTACTCAG AGCCACAGCC CTCCATGTTC TACCAGACAC CGCAGAGTGA GCACATCTAC 
CAGCAGAAGA ACAAGATGCT CATGGAGGTG TACGGCTTCA GCGAGTCCTT CTGCGGTAGT 
GATTCCACGC AGGAGCTGGC CCCTCCACCC GCTCTGCCCC CCAAGCAACG GCAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TATCCGTGTC TAACTCCTTT 
CTCAACCGGC ACGGCAGCTT GCCTGTGCCC TCGGAACCAC CATCTGGGAA GGACGGACAT 
CCCAGAGATC CTTCGGTCAG CAGTGCATCT GGGAAGGACA GCAGAGAAAA TGGGGAAAGG 
TCCCCAAAGT CACTGGATGG TCTGGAGTCA GCGCAGTCAG AAGAGGAAGT GGATGAACTG 
TCCCTCATTG ACCACAATGA AATTATGGCC AGGCTGACAC TCAAGCAAGA GGGTGATGAT 
GGGCCAGATG TTCGTGGTGG TTCAGGAGAC ATCTTACTGG TCCATGCTAC TGAGACAGAC 
AGAAAAGACC TGGTGCTGTA CTGTGAAGCC TTCCTGACCA CCTACAGGAC CTTCATCAGC 
CCGGAGGAGC TCATCAAGAA GCTGCAGTAC CGGTACGAGA AGTTCTCTCC CTTTGCTGAC 
ACGTTCAAGA AGCGAGTGAG CAAGAACACA TTCTTTGTGC TGGTTCGAGT GGTGGATGAG 
CTCTGCCTGG TGGAGCTGAC AGAGGAGATC CTGAAGCTGC TGATGGAGCT GGTCTTCCGC 
CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC AGAGTCCTCC GGAAGAACAT TCTGGACAAG 
GTGGACCAGA AGAAGCTGCT CAGGTGTGCC CATTCCGACC AGCCTCTGGC AGCCAGGGGT 
GTTGCAGCCA GGCCAGGAAC CTTGCATGAT TTCCACAGCC ACGAGATAGC TGAGCAGCTG 
ACACTGCTGG ATGCCGAGCT TTTCTACAAG ATAGAGATTC CTGAAGTTTT GCTTTGGGCC 
AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT CTGACCCAGT TCACAGAGCA CTTCAACAAC 
ATGTCCTACT GGGTGCGGTC CATCATCATG CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG 
CTGCTCCTCA AGTTCATCAA GATCATGAAG CACCTGCGCA AGCTCAACAA CTTCAACTCC 
TACCTGGCCA TCCTCTCAGC ACTAGACTCG GCCCCCATCC GCAGACTGGA GTGGCAGCGG 
CAGACCTCAG AGGGCCTGGC TGAGTACTGC ACATTGATTG ACAGCTCATC CTCCTTCCGA 
GCCTACCGGG CTGCTCTCTC AGAGGTGGAG CCCCCATGTA TCCCATACCT AGGTCTGATT 
CTGCAAGACC TGACCTTCGT TCACCTGGGA AACCCAGATT ATATTGACGG GAAAGTAAAC 
TTCTCCAAGC GGTGGCAACA GTTCAACATA TTGGACAGCA TGCGGTGCTT CCAGCAGGCG 
CACTATGAAA TCCGGAGAAA CGACGACATC ATAAATTTCT TCAATGACTT CAGTGACCAC 
CTGGCCGAGG AAGCCCTGTG GGAACTCTCT CTGAAGATCA AGCCTAGGAA CATAACAAGG 
CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102348.1
CDS	344..3373
Translation	MAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_030246488.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246488.1

ORF Insert Sequence:

ATGGCCATTG ATAAGAAGGT TCTGGAGATG CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG 
GCCATCTTAC CCCTGGTGCA GACTGACCCT CGGATCCAGC ACAGCTCAGC CCTCTCCTCC 
TGTTATAGCC GAGTATACCA GAGCCTCGCC AACCTTATCC GATGGTCTGA CCAGGTGATG 
CTAGAGGGCG TGAACTCAGA AGATAAGGAG ATGGTGACAA CTGTGAAGGG CGTTATCAAA 
GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG AGGCTAACCA TTGAGAAGCA GGGGCGGCCA 
TCGCCAACAA GCCCAGTGAA GCCCAGTTCC CCAGCCAGCA AGCCTGATGG CCAGCCTGAG 
CTCCCTCTGA CAGACCGAGA AATGGAGATT CTGAACAAGA CGACAAGTGT GTCACCATCT 
GCTGAACTGC TCCCAGACTC CACCAGTGAA GAGGTCGCAC CCCCCAAGCC CCCTTTACCT 
GGCATCCGGG TGGTTGATAA CAGTCCACCA GCATTACCAC CCAAGAAAAG GCAGTCTGCT 
CCATCCCCCA CTCGGGTGGC TGTGGTAGCC CCAATGAGTC GGGCTACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGACTTT GATGTTGAAT GTTACACCCA GAGGCGCCTG 
TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG TCTCCTCGCC TGTCCCCCTG CAGCAGCACA 
GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG TCCTCCCTGG ACAGGGATAG TGGGCAGTGC 
TCACGGAACA CAAGCTGTGA AACACTAGAT CACTACGACC CCGACTATGA ATTCCTCCAG 
CAAGATCTCT CCAATGCAGA CCAGATCCCT CCACAGGCAG CCTGTAACCT CAGCCCTCTG 
CCGGAGTCCC TGGGGGAATC TGGGCCTCCA TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG 
GGCAGCTGTC TGCAGCAGGA GGGACAGCAG ACAGACACTC CACCTGCCCT TCCGGAGAAG 
AAGCGTAGGA GCGCAGTCTC CCAGACCACG GACAGCTCTG GCTGCAGGGT GTCCTATGAG 
CGACACCCCT CACAGTATGA CAACATCTCA GAGGGTGACC TGCAGAACCC AGTCCCAGTC 
CAGCCTGTGC CCTACCCACC CTTTGCTGCT GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT 
GCCTCTGCTG AGTTTGTGGG TGATTTCAGT GTTCCTGAGT TGGCGGGTGA CACAGAGAAG 
CCACCTCCTC TACCAGAGAA GAAGAACAAG CACATGCTGG CCTACATGCA ACTGCTGGAG 
GACTACTCAG AGCCACAGCC CTCCATGTTC TACCAGACAC CGCAGAGTGA GCACATCTAC 
CAGCAGAAGA ACAAGATGCT CATGGAGGTG TACGGCTTCA GCGAGTCCTT CTGCGGTAGT 
GATTCCACGC AGGAGCTGGC CCCTCCACCC GCTCTGCCCC CCAAGCAACG GCAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TATCCGTGTC TAACTCCTTT 
CTCAACCGGC ACGGCAGCTT GCCTGTGCCC TCGGAACCAC CATCTGGGAA GGACGGACAT 
CCCAGAGATC CTTCGGTCAG CAGTGCATCT GGGAAGGACA GCAGAGAAAA TGGGGAAAGG 
TCCCCAAAGT CACTGGATGG TCTGGAGTCA GCGCAGTCAG AAGAGGAAGT GGATGAACTG 
TCCCTCATTG ACCACAATGA AATTATGGCC AGGCTGACAC TCAAGCAAGA GGGTGATGAT 
GGGCCAGATG TTCGTGGTGG TTCAGGAGAC ATCTTACTGG TCCATGCTAC TGAGACAGAC 
AGAAAAGACC TGGTGCTGTA CTGTGAAGCC TTCCTGACCA CCTACAGGAC CTTCATCAGC 
CCGGAGGAGC TCATCAAGAA GCTGCAGTAC CGGTACGAGA AGTTCTCTCC CTTTGCTGAC 
ACGTTCAAGA AGCGAGTGAG CAAGAACACA TTCTTTGTGC TGGTTCGAGT GGTGGATGAG 
CTCTGCCTGG TGGAGCTGAC AGAGGAGATC CTGAAGCTGC TGATGGAGCT GGTCTTCCGC 
CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC AGAGTCCTCC GGAAGAACAT TCTGGACAAG 
GTGGACCAGA AGAAGCTGCT CAGGTGTGCC CATTCCGACC AGCCTCTGGC AGCCAGGGGT 
GTTGCAGCCA GGCCAGGAAC CTTGCATGAT TTCCACAGCC ACGAGATAGC TGAGCAGCTG 
ACACTGCTGG ATGCCGAGCT TTTCTACAAG ATAGAGATTC CTGAAGTTTT GCTTTGGGCC 
AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT CTGACCCAGT TCACAGAGCA CTTCAACAAC 
ATGTCCTACT GGGTGCGGTC CATCATCATG CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG 
CTGCTCCTCA AGTTCATCAA GATCATGAAG CACCTGCGCA AGCTCAACAA CTTCAACTCC 
TACCTGGCCA TCCTCTCAGC ACTAGACTCG GCCCCCATCC GCAGACTGGA GTGGCAGCGG 
CAGACCTCAG AGGGCCTGGC TGAGTACTGC ACATTGATTG ACAGCTCATC CTCCTTCCGA 
GCCTACCGGG CTGCTCTCTC AGAGGTGGAG CCCCCATGTA TCCCATACCT AGGTCTGATT 
CTGCAAGACC TGACCTTCGT TCACCTGGGA AACCCAGATT ATATTGACGG GAAAGTAAAC 
TTCTCCAAGC GGTGGCAACA GTTCAACATA TTGGACAGCA TGCGGTGCTT CCAGCAGGCG 
CACTATGAAA TCCGGAGAAA CGACGACATC ATAAATTTCT TCAATGACTT CAGTGACCAC 
CTGGCCGAGG AAGCCCTGTG GGAACTCTCT CTGAAGATCA AGCCTAGGAA CATAACAAGG 
CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( NM_001039086.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034175.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2, mRNA.

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CloneID	OMu00397
Clone ID Related Accession (Same CDS sequence)	NM_001039086.1
Accession Version	NM_001039086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147470.1, AK147696.1 and AK077034.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG CAGGCCTCCT ATGCTGCTTC TTCCTTCTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTATCCGT GTCTAACTCC TTTCTCAACC GGCACGGCAG CTTGCCTGTG 
CCCTCGTACA AATCTGTGTT TAGGTCTTAC TCCCAGGACT TCATGCCTCA CCACCAAGCC 
TCCGTCCAAC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGTCCAC 
ACGTCCCAGA GCTCGGACTT AGCGGTGCCC ACCGTAAGCA GTCCGCCTCC CAGCACCGTG 
GACGGGCCTC TCTCGTCTTC TCAGGACAGC AGCTTTCATG GGAACCCTGT CCGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AAGGCCAGCA GTGAGGAAGC TGGTGGGGAT 
GAGTATGTCA GTCTGTACTC CTCTGGCCAG ACCAGCGAGG AGCTGGCTCC CTGTCGAGGA 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034175.1
CDS	284..3940
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_001039086.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039086.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG CAGGCCTCCT ATGCTGCTTC TTCCTTCTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTATCCGT GTCTAACTCC TTTCTCAACC GGCACGGCAG CTTGCCTGTG 
CCCTCGTACA AATCTGTGTT TAGGTCTTAC TCCCAGGACT TCATGCCTCA CCACCAAGCC 
TCCGTCCAAC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGTCCAC 
ACGTCCCAGA GCTCGGACTT AGCGGTGCCC ACCGTAAGCA GTCCGCCTCC CAGCACCGTG 
GACGGGCCTC TCTCGTCTTC TCAGGACAGC AGCTTTCATG GGAACCCTGT CCGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AAGGCCAGCA GTGAGGAAGC TGGTGGGGAT 
GAGTATGTCA GTCTGTACTC CTCTGGCCAG ACCAGCGAGG AGCTGGCTCC CTGTCGAGGA 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( NM_001039087.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034176.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 1, mRNA.

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CloneID	OMu00400
Clone ID Related Accession (Same CDS sequence)	NM_001039087.1
Accession Version	NM_001039087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147468.1 and AK077034.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147468.1, AK147470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGGA ACCACCATCT GGGAAGGACG GACATCCCAG AGATCCTTCG 
GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AAAGGTCCCC AAAGTCACTG 
GATGGTCTGG AGTCAGCGCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC 
AATGAAATTA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGGCC AGATGTTCGT 
GGTGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTG 
CTGTACTGTG AAGCCTTCCT GACCACCTAC AGGACCTTCA TCAGCCCGGA GGAGCTCATC 
AAGAAGCTGC AGTACCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACGTT CAAGAAGCGA 
GTGAGCAAGA ACACATTCTT TGTGCTGGTT CGAGTGGTGG ATGAGCTCTG CCTGGTGGAG 
CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTAGT GTGCAGCGGA 
GAGCTCAGCC TGGCCAGAGT CCTCCGGAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCCATTC CGACCAGCCT CTGGCAGCCA GGGGTGTTGC AGCCAGGCCA 
GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAGC AGCTGACACT GCTGGATGCC 
GAGCTTTTCT ACAAGATAGA GATTCCTGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAG 
GAGAAGAGTC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG 
CGGTCCATCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTCAAGTTC 
ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAACTTCA ACTCCTACCT GGCCATCCTC 
TCAGCACTAG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGGCAGAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACATT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCT 
CTCTCAGAGG TGGAGCCCCC ATGTATCCCA TACCTAGGTC TGATTCTGCA AGACCTGACC 
TTCGTTCACC TGGGAAACCC AGATTATATT GACGGGAAAG TAAACTTCTC CAAGCGGTGG 
CAACAGTTCA ACATATTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGAAATCCGG 
AGAAACGACG ACATCATAAA TTTCTTCAAT GACTTCAGTG ACCACCTGGC CGAGGAAGCC 
CTGTGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGC 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034176.1
CDS	284..3958
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_001039087.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039087.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGGA ACCACCATCT GGGAAGGACG GACATCCCAG AGATCCTTCG 
GTCAGCAGTG CATCTGGGAA GGACAGCAGA GAAAATGGGG AAAGGTCCCC AAAGTCACTG 
GATGGTCTGG AGTCAGCGCA GTCAGAAGAG GAAGTGGATG AACTGTCCCT CATTGACCAC 
AATGAAATTA TGGCCAGGCT GACACTCAAG CAAGAGGGTG ATGATGGGCC AGATGTTCGT 
GGTGGTTCAG GAGACATCTT ACTGGTCCAT GCTACTGAGA CAGACAGAAA AGACCTGGTG 
CTGTACTGTG AAGCCTTCCT GACCACCTAC AGGACCTTCA TCAGCCCGGA GGAGCTCATC 
AAGAAGCTGC AGTACCGGTA CGAGAAGTTC TCTCCCTTTG CTGACACGTT CAAGAAGCGA 
GTGAGCAAGA ACACATTCTT TGTGCTGGTT CGAGTGGTGG ATGAGCTCTG CCTGGTGGAG 
CTGACAGAGG AGATCCTGAA GCTGCTGATG GAGCTGGTCT TCCGCCTAGT GTGCAGCGGA 
GAGCTCAGCC TGGCCAGAGT CCTCCGGAAG AACATTCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCCATTC CGACCAGCCT CTGGCAGCCA GGGGTGTTGC AGCCAGGCCA 
GGAACCTTGC ATGATTTCCA CAGCCACGAG ATAGCTGAGC AGCTGACACT GCTGGATGCC 
GAGCTTTTCT ACAAGATAGA GATTCCTGAA GTTTTGCTTT GGGCCAAAGA GCAGAATGAG 
GAGAAGAGTC CCAATCTGAC CCAGTTCACA GAGCACTTCA ACAACATGTC CTACTGGGTG 
CGGTCCATCA TCATGCTGCA GGAGAAGGCC CAGGACCGGG AGAGGCTGCT CCTCAAGTTC 
ATCAAGATCA TGAAGCACCT GCGCAAGCTC AACAACTTCA ACTCCTACCT GGCCATCCTC 
TCAGCACTAG ACTCGGCCCC CATCCGCAGA CTGGAGTGGC AGCGGCAGAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACATT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCTGCT 
CTCTCAGAGG TGGAGCCCCC ATGTATCCCA TACCTAGGTC TGATTCTGCA AGACCTGACC 
TTCGTTCACC TGGGAAACCC AGATTATATT GACGGGAAAG TAAACTTCTC CAAGCGGTGG 
CAACAGTTCA ACATATTGGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGAAATCCGG 
AGAAACGACG ACATCATAAA TTTCTTCAAT GACTTCAGTG ACCACCTGGC CGAGGAAGCC 
CTGTGGGAAC TCTCTCTGAA GATCAAGCCT AGGAACATAA CAAGGCGGAA AACAGACCGC 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( NM_054050.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473391.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 3, mRNA.

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CloneID	OMu09514
Clone ID Related Accession (Same CDS sequence)	NM_054050.2
Accession Version	NM_054050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147470.1, AF348669.1 and AK077034.1. On Jan 14, 2006 this sequence version replaced NM_054050.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF348669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGGAACCA CCATCTGGGA AGGACGGACA TCCCAGAGAT 
CCTTCGGTCA GCAGTGCATC TGGGAAGGAC AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG 
TCACTGGATG GTCTGGAGTC AGCGCAGTCA GAAGAGGAAG TGGATGAACT GTCCCTCATT 
GACCACAATG AAATTATGGC CAGGCTGACA CTCAAGCAAG AGGGTGATGA TGGGCCAGAT 
GTTCGTGGTG GTTCAGGAGA CATCTTACTG GTCCATGCTA CTGAGACAGA CAGAAAAGAC 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACC ACCTACAGGA CCTTCATCAG CCCGGAGGAG 
CTCATCAAGA AGCTGCAGTA CCGGTACGAG AAGTTCTCTC CCTTTGCTGA CACGTTCAAG 
AAGCGAGTGA GCAAGAACAC ATTCTTTGTG CTGGTTCGAG TGGTGGATGA GCTCTGCCTG 
GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTAGTGTGC 
AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC CGGAAGAACA TTCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CCATTCCGAC CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC 
AGGCCAGGAA CCTTGCATGA TTTCCACAGC CACGAGATAG CTGAGCAGCT GACACTGCTG 
GATGCCGAGC TTTTCTACAA GATAGAGATT CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG 
AATGAGGAGA AGAGTCCCAA TCTGACCCAG TTCACAGAGC ACTTCAACAA CATGTCCTAC 
TGGGTGCGGT CCATCATCAT GCTGCAGGAG AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC 
AAGTTCATCA AGATCATGAA GCACCTGCGC AAGCTCAACA ACTTCAACTC CTACCTGGCC 
ATCCTCTCAG CACTAGACTC GGCCCCCATC CGCAGACTGG AGTGGCAGCG GCAGACCTCA 
GAGGGCCTGG CTGAGTACTG CACATTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCTGCTCTCT CAGAGGTGGA GCCCCCATGT ATCCCATACC TAGGTCTGAT TCTGCAAGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAT TATATTGACG GGAAAGTAAA CTTCTCCAAG 
CGGTGGCAAC AGTTCAACAT ATTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAA 
ATCCGGAGAA ACGACGACAT CATAAATTTC TTCAATGACT TCAGTGACCA CCTGGCCGAG 
GAAGCCCTGT GGGAACTCTC TCTGAAGATC AAGCCTAGGA ACATAACAAG GCGGAAAACA 
GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473391.1
CDS	284..3544
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_054050.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054050.2

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGGAACCA CCATCTGGGA AGGACGGACA TCCCAGAGAT 
CCTTCGGTCA GCAGTGCATC TGGGAAGGAC AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG 
TCACTGGATG GTCTGGAGTC AGCGCAGTCA GAAGAGGAAG TGGATGAACT GTCCCTCATT 
GACCACAATG AAATTATGGC CAGGCTGACA CTCAAGCAAG AGGGTGATGA TGGGCCAGAT 
GTTCGTGGTG GTTCAGGAGA CATCTTACTG GTCCATGCTA CTGAGACAGA CAGAAAAGAC 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACC ACCTACAGGA CCTTCATCAG CCCGGAGGAG 
CTCATCAAGA AGCTGCAGTA CCGGTACGAG AAGTTCTCTC CCTTTGCTGA CACGTTCAAG 
AAGCGAGTGA GCAAGAACAC ATTCTTTGTG CTGGTTCGAG TGGTGGATGA GCTCTGCCTG 
GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG CTGATGGAGC TGGTCTTCCG CCTAGTGTGC 
AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC CGGAAGAACA TTCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CCATTCCGAC CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC 
AGGCCAGGAA CCTTGCATGA TTTCCACAGC CACGAGATAG CTGAGCAGCT GACACTGCTG 
GATGCCGAGC TTTTCTACAA GATAGAGATT CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG 
AATGAGGAGA AGAGTCCCAA TCTGACCCAG TTCACAGAGC ACTTCAACAA CATGTCCTAC 
TGGGTGCGGT CCATCATCAT GCTGCAGGAG AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC 
AAGTTCATCA AGATCATGAA GCACCTGCGC AAGCTCAACA ACTTCAACTC CTACCTGGCC 
ATCCTCTCAG CACTAGACTC GGCCCCCATC CGCAGACTGG AGTGGCAGCG GCAGACCTCA 
GAGGGCCTGG CTGAGTACTG CACATTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCTGCTCTCT CAGAGGTGGA GCCCCCATGT ATCCCATACC TAGGTCTGAT TCTGCAAGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAT TATATTGACG GGAAAGTAAA CTTCTCCAAG 
CGGTGGCAAC AGTTCAACAT ATTGGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAA 
ATCCGGAGAA ACGACGACAT CATAAATTTC TTCAATGACT TCAGTGACCA CCTGGCCGAG 
GAAGCCCTGT GGGAACTCTC TCTGAAGATC AAGCCTAGGA ACATAACAAG GCGGAAAACA 
GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( NM_001362702.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349631.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 4, mRNA.

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CloneID	OMu29226
Clone ID Related Accession (Same CDS sequence)	XM_006497588.1 , NM_001362702.1
Accession Version	NM_001362702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147468.1, BC129881.1 and AK077034.1. On Apr 25, 2018 this sequence version replaced XM_006497588.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC129881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349631.1
CDS	284..3841
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_001362702.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362702.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497582.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497645.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X1, mRNA.

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CloneID	OMu29220
Clone ID Related Accession (Same CDS sequence)	XM_006497582.3
Accession Version	XM_006497582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAG GCCAGCAGTG AGGAAGCTGG TGGGGATGAG 
TATGTCAGTC TGTACTCCTC TGGCCAGACC AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA 
CCACCATCTG GGAAGGACGG ACATCCCAGA GATCCTTCGG TCAGCAGTGC ATCTGGGAAG 
GACAGCAGAG AAAATGGGGA AAGGTCCCCA AAGTCACTGG ATGGTCTGGA GTCAGCGCAG 
TCAGAAGAGG AAGTGGATGA ACTGTCCCTC ATTGACCACA ATGAAATTAT GGCCAGGCTG 
ACACTCAAGC AAGAGGGTGA TGATGGGCCA GATGTTCGTG GTGGTTCAGG AGACATCTTA 
CTGGTCCATG CTACTGAGAC AGACAGAAAA GACCTGGTGC TGTACTGTGA AGCCTTCCTG 
ACCACCTACA GGACCTTCAT CAGCCCGGAG GAGCTCATCA AGAAGCTGCA GTACCGGTAC 
GAGAAGTTCT CTCCCTTTGC TGACACGTTC AAGAAGCGAG TGAGCAAGAA CACATTCTTT 
GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC CTGGTGGAGC TGACAGAGGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTAGTG TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC 
CTCCGGAAGA ACATTCTGGA CAAGGTGGAC CAGAAGAAGC TGCTCAGGTG TGCCCATTCC 
GACCAGCCTC TGGCAGCCAG GGGTGTTGCA GCCAGGCCAG GAACCTTGCA TGATTTCCAC 
AGCCACGAGA TAGCTGAGCA GCTGACACTG CTGGATGCCG AGCTTTTCTA CAAGATAGAG 
ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG CAGAATGAGG AGAAGAGTCC CAATCTGACC 
CAGTTCACAG AGCACTTCAA CAACATGTCC TACTGGGTGC GGTCCATCAT CATGCTGCAG 
GAGAAGGCCC AGGACCGGGA GAGGCTGCTC CTCAAGTTCA TCAAGATCAT GAAGCACCTG 
CGCAAGCTCA ACAACTTCAA CTCCTACCTG GCCATCCTCT CAGCACTAGA CTCGGCCCCC 
ATCCGCAGAC TGGAGTGGCA GCGGCAGACC TCAGAGGGCC TGGCTGAGTA CTGCACATTG 
ATTGACAGCT CATCCTCCTT CCGAGCCTAC CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA 
TGTATCCCAT ACCTAGGTCT GATTCTGCAA GACCTGACCT TCGTTCACCT GGGAAACCCA 
GATTATATTG ACGGGAAAGT AAACTTCTCC AAGCGGTGGC AACAGTTCAA CATATTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAT GAAATCCGGA GAAACGACGA CATCATAAAT 
TTCTTCAATG ACTTCAGTGA CCACCTGGCC GAGGAAGCCC TGTGGGAACT CTCTCTGAAG 
ATCAAGCCTA GGAACATAAC AAGGCGGAAA ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497645.1
CDS	166..3939
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497582.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497582.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAG GCCAGCAGTG AGGAAGCTGG TGGGGATGAG 
TATGTCAGTC TGTACTCCTC TGGCCAGACC AGCGAGGAGC TGGCTCCCTG TCGAGGAGAA 
CCACCATCTG GGAAGGACGG ACATCCCAGA GATCCTTCGG TCAGCAGTGC ATCTGGGAAG 
GACAGCAGAG AAAATGGGGA AAGGTCCCCA AAGTCACTGG ATGGTCTGGA GTCAGCGCAG 
TCAGAAGAGG AAGTGGATGA ACTGTCCCTC ATTGACCACA ATGAAATTAT GGCCAGGCTG 
ACACTCAAGC AAGAGGGTGA TGATGGGCCA GATGTTCGTG GTGGTTCAGG AGACATCTTA 
CTGGTCCATG CTACTGAGAC AGACAGAAAA GACCTGGTGC TGTACTGTGA AGCCTTCCTG 
ACCACCTACA GGACCTTCAT CAGCCCGGAG GAGCTCATCA AGAAGCTGCA GTACCGGTAC 
GAGAAGTTCT CTCCCTTTGC TGACACGTTC AAGAAGCGAG TGAGCAAGAA CACATTCTTT 
GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC CTGGTGGAGC TGACAGAGGA GATCCTGAAG 
CTGCTGATGG AGCTGGTCTT CCGCCTAGTG TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC 
CTCCGGAAGA ACATTCTGGA CAAGGTGGAC CAGAAGAAGC TGCTCAGGTG TGCCCATTCC 
GACCAGCCTC TGGCAGCCAG GGGTGTTGCA GCCAGGCCAG GAACCTTGCA TGATTTCCAC 
AGCCACGAGA TAGCTGAGCA GCTGACACTG CTGGATGCCG AGCTTTTCTA CAAGATAGAG 
ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG CAGAATGAGG AGAAGAGTCC CAATCTGACC 
CAGTTCACAG AGCACTTCAA CAACATGTCC TACTGGGTGC GGTCCATCAT CATGCTGCAG 
GAGAAGGCCC AGGACCGGGA GAGGCTGCTC CTCAAGTTCA TCAAGATCAT GAAGCACCTG 
CGCAAGCTCA ACAACTTCAA CTCCTACCTG GCCATCCTCT CAGCACTAGA CTCGGCCCCC 
ATCCGCAGAC TGGAGTGGCA GCGGCAGACC TCAGAGGGCC TGGCTGAGTA CTGCACATTG 
ATTGACAGCT CATCCTCCTT CCGAGCCTAC CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA 
TGTATCCCAT ACCTAGGTCT GATTCTGCAA GACCTGACCT TCGTTCACCT GGGAAACCCA 
GATTATATTG ACGGGAAAGT AAACTTCTCC AAGCGGTGGC AACAGTTCAA CATATTGGAC 
AGCATGCGGT GCTTCCAGCA GGCGCACTAT GAAATCCGGA GAAACGACGA CATCATAAAT 
TTCTTCAATG ACTTCAGTGA CCACCTGGCC GAGGAAGCCC TGTGGGAACT CTCTCTGAAG 
ATCAAGCCTA GGAACATAAC AAGGCGGAAA ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497583.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497646.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.

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CloneID	OMu29221
Clone ID Related Accession (Same CDS sequence)	XM_006497583.2 , XM_006497583.1
Accession Version	XM_006497583.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497646.1
CDS	279..4049
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497583.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497583.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAGA GGCAAGAGAC AGATTTCTAC CAGAGGGCTA CCCTATCCCC 
TTGGATCTGG AGCAGCAGGC AGTAGAATTT ATGTCCACCA GTGCTGTGGC TTCCAGGTCT 
CAGAGGCAGA AGAACCTGTG CTGGCTGGAG GAGAAAGAGA AGGAGGTTGT CAGTGCCTTG 
CGCTACTTTA AGACCATTGT GGACAAAATG GCCATTGATA AGAAGGTTCT GGAGATGCTC 
CCGGGGTCAG CCAGCAAGGT GCTGGAGGCC ATCTTACCCC TGGTGCAGAC TGACCCTCGG 
ATCCAGCACA GCTCAGCCCT CTCCTCCTGT TATAGCCGAG TATACCAGAG CCTCGCCAAC 
CTTATCCGAT GGTCTGACCA GGTGATGCTA GAGGGCGTGA ACTCAGAAGA TAAGGAGATG 
GTGACAACTG TGAAGGGCGT TATCAAAGCT GTCCTGGACG GAGTGAAGGA GCTAGTGAGG 
CTAACCATTG AGAAGCAGGG GCGGCCATCG CCAACAAGCC CAGTGAAGCC CAGTTCCCCA 
GCCAGCAAGC CTGATGGCCA GCCTGAGCTC CCTCTGACAG ACCGAGAAAT GGAGATTCTG 
AACAAGACGA CAAGTGTGTC ACCATCTGCT GAACTGCTCC CAGACTCCAC CAGTGAAGAG 
GTCGCACCCC CCAAGCCCCC TTTACCTGGC ATCCGGGTGG TTGATAACAG TCCACCAGCA 
TTACCACCCA AGAAAAGGCA GTCTGCTCCA TCCCCCACTC GGGTGGCTGT GGTAGCCCCA 
ATGAGTCGGG CTACCAGTGG CTCCAGTTTG CCTGTTGGAA TCAATAGGCA GGACTTTGAT 
GTTGAATGTT ACACCCAGAG GCGCCTGTCA GGAGGCAGCC GCTCCTGCGG TGGTGAGTCT 
CCTCGCCTGT CCCCCTGCAG CAGCACAGGC AAGCTCAGCC GCTCAGACGA GCAGCTGTCC 
TCCCTGGACA GGGATAGTGG GCAGTGCTCA CGGAACACAA GCTGTGAAAC ACTAGATCAC 
TACGACCCCG ACTATGAATT CCTCCAGCAA GATCTCTCCA ATGCAGACCA GATCCCTCCA 
CAGGCAGCCT GTAACCTCAG CCCTCTGCCG GAGTCCCTGG GGGAATCTGG GCCTCCATTT 
CTTGGCCACC CTTTCCAGCT GCCTTTGGGC AGCTGTCTGC AGCAGGAGGG ACAGCAGACA 
GACACTCCAC CTGCCCTTCC GGAGAAGAAG CGTAGGAGCG CAGTCTCCCA GACCACGGAC 
AGCTCTGGCT GCAGGGTGTC CTATGAGCGA CACCCCTCAC AGTATGACAA CATCTCAGAG 
GGTGACCTGC AGAACCCAGT CCCAGTCCAG CCTGTGCCCT ACCCACCCTT TGCTGCTGTC 
CTGCCCTTTC AGCAGGGAGC TTCCTCTGCC TCTGCTGAGT TTGTGGGTGA TTTCAGTGTT 
CCTGAGTTGG CGGGTGACAC AGAGAAGCCA CCTCCTCTAC CAGAGAAGAA GAACAAGCAC 
ATGCTGGCCT ACATGCAACT GCTGGAGGAC TACTCAGAGC CACAGCCCTC CATGTTCTAC 
CAGACACCGC AGAGTGAGCA CATCTACCAG CAGAAGAACA AGATGCTCAT GGAGGTGTAC 
GGCTTCAGCG AGTCCTTCTG CGGTAGTGAT TCCACGCAGG AGCTGGCCCC TCCACCCGCT 
CTGCCCCCCA AGCAACGGCA GCTGCAGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497584.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497647.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X3, mRNA.

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CloneID	OMu29222
Clone ID Related Accession (Same CDS sequence)	XM_006497584.3
Accession Version	XM_006497584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497647.1
CDS	166..3936
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497584.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497584.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
TACAAATCTG TGTTTAGGTC TTACTCCCAG GACTTCATGC CTCACCACCA AGCCTCCGTC 
CAACCTTTCC TTCCGCCTAC CTCCTCTTCC TCTCCACATT TCCCACCTGT CCACACGTCC 
CAGAGCTCGG ACTTAGCGGT GCCCACCGTA AGCAGTCCGC CTCCCAGCAC CGTGGACGGG 
CCTCTCTCGT CTTCTCAGGA CAGCAGCTTT CATGGGAACC CTGTCCGCCT TCCTTCCGAA 
ACCTCTTTCA CTGACTCGAG TGAAAAGGCC AGCAGTGAGG AAGCTGGTGG GGATGAGTAT 
GTCAGTCTGT ACTCCTCTGG CCAGACCAGC GAGGAGCTGG CTCCCTGTCG AGGAGAACCA 
CCATCTGGGA AGGACGGACA TCCCAGAGAT CCTTCGGTCA GCAGTGCATC TGGGAAGGAC 
AGCAGAGAAA ATGGGGAAAG GTCCCCAAAG TCACTGGATG GTCTGGAGTC AGCGCAGTCA 
GAAGAGGAAG TGGATGAACT GTCCCTCATT GACCACAATG AAATTATGGC CAGGCTGACA 
CTCAAGCAAG AGGGTGATGA TGGGCCAGAT GTTCGTGGTG GTTCAGGAGA CATCTTACTG 
GTCCATGCTA CTGAGACAGA CAGAAAAGAC CTGGTGCTGT ACTGTGAAGC CTTCCTGACC 
ACCTACAGGA CCTTCATCAG CCCGGAGGAG CTCATCAAGA AGCTGCAGTA CCGGTACGAG 
AAGTTCTCTC CCTTTGCTGA CACGTTCAAG AAGCGAGTGA GCAAGAACAC ATTCTTTGTG 
CTGGTTCGAG TGGTGGATGA GCTCTGCCTG GTGGAGCTGA CAGAGGAGAT CCTGAAGCTG 
CTGATGGAGC TGGTCTTCCG CCTAGTGTGC AGCGGAGAGC TCAGCCTGGC CAGAGTCCTC 
CGGAAGAACA TTCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CCATTCCGAC 
CAGCCTCTGG CAGCCAGGGG TGTTGCAGCC AGGCCAGGAA CCTTGCATGA TTTCCACAGC 
CACGAGATAG CTGAGCAGCT GACACTGCTG GATGCCGAGC TTTTCTACAA GATAGAGATT 
CCTGAAGTTT TGCTTTGGGC CAAAGAGCAG AATGAGGAGA AGAGTCCCAA TCTGACCCAG 
TTCACAGAGC ACTTCAACAA CATGTCCTAC TGGGTGCGGT CCATCATCAT GCTGCAGGAG 
AAGGCCCAGG ACCGGGAGAG GCTGCTCCTC AAGTTCATCA AGATCATGAA GCACCTGCGC 
AAGCTCAACA ACTTCAACTC CTACCTGGCC ATCCTCTCAG CACTAGACTC GGCCCCCATC 
CGCAGACTGG AGTGGCAGCG GCAGACCTCA GAGGGCCTGG CTGAGTACTG CACATTGATT 
GACAGCTCAT CCTCCTTCCG AGCCTACCGG GCTGCTCTCT CAGAGGTGGA GCCCCCATGT 
ATCCCATACC TAGGTCTGAT TCTGCAAGAC CTGACCTTCG TTCACCTGGG AAACCCAGAT 
TATATTGACG GGAAAGTAAA CTTCTCCAAG CGGTGGCAAC AGTTCAACAT ATTGGACAGC 
ATGCGGTGCT TCCAGCAGGC GCACTATGAA ATCCGGAGAA ACGACGACAT CATAAATTTC 
TTCAATGACT TCAGTGACCA CCTGGCCGAG GAAGCCCTGT GGGAACTCTC TCTGAAGATC 
AAGCCTAGGA ACATAACAAG GCGGAAAACA GACCGCGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497648.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu29223
Clone ID Related Accession (Same CDS sequence)	XM_006497585.3 , XM_006497585.4
Accession Version	XM_006497585.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497648.1
CDS	165..3842
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497585.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497585.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497586.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497649.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.

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CloneID	OMu29224
Clone ID Related Accession (Same CDS sequence)	XM_006497586.3 , XM_006497586.4
Accession Version	XM_006497586.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGCAGGCCT CCTATGCTGC TTCTTCCTTC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCC TCAGTGTATC CGTGTCTAAC TCCTTTCTCA ACCGGCACGG CAGCTTGCCT 
GTGCCCTCGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA 
GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC 
CACACGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC 
GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAAGGCCA GCAGTGAGGA AGCTGGTGGG 
GATGAGTATG TCAGTCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTGGC TCCCTGTCGA 
GGAGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497649.1
CDS	165..3824
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497586.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497586.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AAACCTGTGC TGGCTGGAGG AGAAAGAGAA GGAGGTTGTC 
AGTGCCTTGC GCTACTTTAA GACCATTGTG GACAAAATGG CCATTGATAA GAAGGTTCTG 
GAGATGCTCC CGGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGACT 
GACCCTCGGA TCCAGCACAG CTCAGCCCTC TCCTCCTGTT ATAGCCGAGT ATACCAGAGC 
CTCGCCAACC TTATCCGATG GTCTGACCAG GTGATGCTAG AGGGCGTGAA CTCAGAAGAT 
AAGGAGATGG TGACAACTGT GAAGGGCGTT ATCAAAGCTG TCCTGGACGG AGTGAAGGAG 
CTAGTGAGGC TAACCATTGA GAAGCAGGGG CGGCCATCGC CAACAAGCCC AGTGAAGCCC 
AGTTCCCCAG CCAGCAAGCC TGATGGCCAG CCTGAGCTCC CTCTGACAGA CCGAGAAATG 
GAGATTCTGA ACAAGACGAC AAGTGTGTCA CCATCTGCTG AACTGCTCCC AGACTCCACC 
AGTGAAGAGG TCGCACCCCC CAAGCCCCCT TTACCTGGCA TCCGGGTGGT TGATAACAGT 
CCACCAGCAT TACCACCCAA GAAAAGGCAG TCTGCTCCAT CCCCCACTCG GGTGGCTGTG 
GTAGCCCCAA TGAGTCGGGC TACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GACTTTGATG TTGAATGTTA CACCCAGAGG CGCCTGTCAG GAGGCAGCCG CTCCTGCGGT 
GGTGAGTCTC CTCGCCTGTC CCCCTGCAGC AGCACAGGCA AGCTCAGCCG CTCAGACGAG 
CAGCTGTCCT CCCTGGACAG GGATAGTGGG CAGTGCTCAC GGAACACAAG CTGTGAAACA 
CTAGATCACT ACGACCCCGA CTATGAATTC CTCCAGCAAG ATCTCTCCAA TGCAGACCAG 
ATCCCTCCAC AGGCAGCCTG TAACCTCAGC CCTCTGCCGG AGTCCCTGGG GGAATCTGGG 
CCTCCATTTC TTGGCCACCC TTTCCAGCTG CCTTTGGGCA GCTGTCTGCA GCAGGAGGGA 
CAGCAGACAG ACACTCCACC TGCCCTTCCG GAGAAGAAGC GTAGGAGCGC AGTCTCCCAG 
ACCACGGACA GCTCTGGCTG CAGGGTGTCC TATGAGCGAC ACCCCTCACA GTATGACAAC 
ATCTCAGAGG GTGACCTGCA GAACCCAGTC CCAGTCCAGC CTGTGCCCTA CCCACCCTTT 
GCTGCTGTCC TGCCCTTTCA GCAGGGAGCT TCCTCTGCCT CTGCTGAGTT TGTGGGTGAT 
TTCAGTGTTC CTGAGTTGGC GGGTGACACA GAGAAGCCAC CTCCTCTACC AGAGAAGAAG 
AACAAGCACA TGCTGGCCTA CATGCAACTG CTGGAGGACT ACTCAGAGCC ACAGCCCTCC 
ATGTTCTACC AGACACCGCA GAGTGAGCAC ATCTACCAGC AGAAGAACAA GATGCTCATG 
GAGGTGTACG GCTTCAGCGA GTCCTTCTGC GGTAGTGATT CCACGCAGGA GCTGGCCCCT 
CCACCCGCTC TGCCCCCCAA GCAACGGCAG CTGCAGGCCT CCTATGCTGC TTCTTCCTTC 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC 
GCTCAGGGCC TCAGTGTATC CGTGTCTAAC TCCTTTCTCA ACCGGCACGG CAGCTTGCCT 
GTGCCCTCGT ACAAATCTGT GTTTAGGTCT TACTCCCAGG ACTTCATGCC TCACCACCAA 
GCCTCCGTCC AACCTTTCCT TCCGCCTACC TCCTCTTCCT CTCCACATTT CCCACCTGTC 
CACACGTCCC AGAGCTCGGA CTTAGCGGTG CCCACCGTAA GCAGTCCGCC TCCCAGCACC 
GTGGACGGGC CTCTCTCGTC TTCTCAGGAC AGCAGCTTTC ATGGGAACCC TGTCCGCCTT 
CCTTCCGAAA CCTCTTTCAC TGACTCGAGT GAAAAGGCCA GCAGTGAGGA AGCTGGTGGG 
GATGAGTATG TCAGTCTGTA CTCCTCTGGC CAGACCAGCG AGGAGCTGGC TCCCTGTCGA 
GGAGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497587.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497650.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu29225
Clone ID Related Accession (Same CDS sequence)	XM_006497587.3 , XM_006497587.4
Accession Version	XM_006497587.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC 
TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CACGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC 
AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT 
GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAAGGCCAGC 
AGTGAGGAAG CTGGTGGGGA TGAGTATGTC AGTCTGTACT CCTCTGGCCA GACCAGCGAG 
GAGCTGGCTC CCTGTCGAGG AGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497650.1
CDS	166..3783
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497587.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497587.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGTAC AAATCTGTGT TTAGGTCTTA CTCCCAGGAC 
TTCATGCCTC ACCACCAAGC CTCCGTCCAA CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CACGTCCCAG AGCTCGGACT TAGCGGTGCC CACCGTAAGC 
AGTCCGCCTC CCAGCACCGT GGACGGGCCT CTCTCGTCTT CTCAGGACAG CAGCTTTCAT 
GGGAACCCTG TCCGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAAGGCCAGC 
AGTGAGGAAG CTGGTGGGGA TGAGTATGTC AGTCTGTACT CCTCTGGCCA GACCAGCGAG 
GAGCTGGCTC CCTGTCGAGG AGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497588.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497651.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.

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CloneID	OMu29226
Clone ID Related Accession (Same CDS sequence)	XM_006497588.1 , NM_001362702.1
Accession Version	XM_006497588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497651.1
CDS	279..3836
Translation	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497588.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497588.1

ORF Insert Sequence:

ATGAGCAGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCCTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCT 
CCCAAAATCA AGAGAACACC ATCCAAGAAG GGAAAGCCAG CCGAGGTGTC GAAGATTCCT 
GAGAAGCCTG TGAGCAAAAA CCTGTGCTGG CTGGAGGAGA AAGAGAAGGA GGTTGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCCA TTGATAAGAA GGTTCTGGAG 
ATGCTCCCGG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGACTGAC 
CCTCGGATCC AGCACAGCTC AGCCCTCTCC TCCTGTTATA GCCGAGTATA CCAGAGCCTC 
GCCAACCTTA TCCGATGGTC TGACCAGGTG ATGCTAGAGG GCGTGAACTC AGAAGATAAG 
GAGATGGTGA CAACTGTGAA GGGCGTTATC AAAGCTGTCC TGGACGGAGT GAAGGAGCTA 
GTGAGGCTAA CCATTGAGAA GCAGGGGCGG CCATCGCCAA CAAGCCCAGT GAAGCCCAGT 
TCCCCAGCCA GCAAGCCTGA TGGCCAGCCT GAGCTCCCTC TGACAGACCG AGAAATGGAG 
ATTCTGAACA AGACGACAAG TGTGTCACCA TCTGCTGAAC TGCTCCCAGA CTCCACCAGT 
GAAGAGGTCG CACCCCCCAA GCCCCCTTTA CCTGGCATCC GGGTGGTTGA TAACAGTCCA 
CCAGCATTAC CACCCAAGAA AAGGCAGTCT GCTCCATCCC CCACTCGGGT GGCTGTGGTA 
GCCCCAATGA GTCGGGCTAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAC 
TTTGATGTTG AATGTTACAC CCAGAGGCGC CTGTCAGGAG GCAGCCGCTC CTGCGGTGGT 
GAGTCTCCTC GCCTGTCCCC CTGCAGCAGC ACAGGCAAGC TCAGCCGCTC AGACGAGCAG 
CTGTCCTCCC TGGACAGGGA TAGTGGGCAG TGCTCACGGA ACACAAGCTG TGAAACACTA 
GATCACTACG ACCCCGACTA TGAATTCCTC CAGCAAGATC TCTCCAATGC AGACCAGATC 
CCTCCACAGG CAGCCTGTAA CCTCAGCCCT CTGCCGGAGT CCCTGGGGGA ATCTGGGCCT 
CCATTTCTTG GCCACCCTTT CCAGCTGCCT TTGGGCAGCT GTCTGCAGCA GGAGGGACAG 
CAGACAGACA CTCCACCTGC CCTTCCGGAG AAGAAGCGTA GGAGCGCAGT CTCCCAGACC 
ACGGACAGCT CTGGCTGCAG GGTGTCCTAT GAGCGACACC CCTCACAGTA TGACAACATC 
TCAGAGGGTG ACCTGCAGAA CCCAGTCCCA GTCCAGCCTG TGCCCTACCC ACCCTTTGCT 
GCTGTCCTGC CCTTTCAGCA GGGAGCTTCC TCTGCCTCTG CTGAGTTTGT GGGTGATTTC 
AGTGTTCCTG AGTTGGCGGG TGACACAGAG AAGCCACCTC CTCTACCAGA GAAGAAGAAC 
AAGCACATGC TGGCCTACAT GCAACTGCTG GAGGACTACT CAGAGCCACA GCCCTCCATG 
TTCTACCAGA CACCGCAGAG TGAGCACATC TACCAGCAGA AGAACAAGAT GCTCATGGAG 
GTGTACGGCT TCAGCGAGTC CTTCTGCGGT AGTGATTCCA CGCAGGAGCT GGCCCCTCCA 
CCCGCTCTGC CCCCCAAGCA ACGGCAGCTG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGTACAAAT CTGTGTTTAG GTCTTACTCC CAGGACTTCA TGCCTCACCA CCAAGCCTCC 
GTCCAACCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGTCCACACG 
TCCCAGAGCT CGGACTTAGC GGTGCCCACC GTAAGCAGTC CGCCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGTCTTCTCA GGACAGCAGC TTTCATGGGA ACCCTGTCCG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAACCACCA TCTGGGAAGG ACGGACATCC CAGAGATCCT 
TCGGTCAGCA GTGCATCTGG GAAGGACAGC AGAGAAAATG GGGAAAGGTC CCCAAAGTCA 
CTGGATGGTC TGGAGTCAGC GCAGTCAGAA GAGGAAGTGG ATGAACTGTC CCTCATTGAC 
CACAATGAAA TTATGGCCAG GCTGACACTC AAGCAAGAGG GTGATGATGG GCCAGATGTT 
CGTGGTGGTT CAGGAGACAT CTTACTGGTC CATGCTACTG AGACAGACAG AAAAGACCTG 
GTGCTGTACT GTGAAGCCTT CCTGACCACC TACAGGACCT TCATCAGCCC GGAGGAGCTC 
ATCAAGAAGC TGCAGTACCG GTACGAGAAG TTCTCTCCCT TTGCTGACAC GTTCAAGAAG 
CGAGTGAGCA AGAACACATT CTTTGTGCTG GTTCGAGTGG TGGATGAGCT CTGCCTGGTG 
GAGCTGACAG AGGAGATCCT GAAGCTGCTG ATGGAGCTGG TCTTCCGCCT AGTGTGCAGC 
GGAGAGCTCA GCCTGGCCAG AGTCCTCCGG AAGAACATTC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCCA TTCCGACCAG CCTCTGGCAG CCAGGGGTGT TGCAGCCAGG 
CCAGGAACCT TGCATGATTT CCACAGCCAC GAGATAGCTG AGCAGCTGAC ACTGCTGGAT 
GCCGAGCTTT TCTACAAGAT AGAGATTCCT GAAGTTTTGC TTTGGGCCAA AGAGCAGAAT 
GAGGAGAAGA GTCCCAATCT GACCCAGTTC ACAGAGCACT TCAACAACAT GTCCTACTGG 
GTGCGGTCCA TCATCATGCT GCAGGAGAAG GCCCAGGACC GGGAGAGGCT GCTCCTCAAG 
TTCATCAAGA TCATGAAGCA CCTGCGCAAG CTCAACAACT TCAACTCCTA CCTGGCCATC 
CTCTCAGCAC TAGACTCGGC CCCCATCCGC AGACTGGAGT GGCAGCGGCA GACCTCAGAG 
GGCCTGGCTG AGTACTGCAC ATTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCT 
GCTCTCTCAG AGGTGGAGCC CCCATGTATC CCATACCTAG GTCTGATTCT GCAAGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGATTAT ATTGACGGGA AAGTAAACTT CTCCAAGCGG 
TGGCAACAGT TCAACATATT GGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGAAATC 
CGGAGAAACG ACGACATCAT AAATTTCTTC AATGACTTCA GTGACCACCT GGCCGAGGAA 
GCCCTGTGGG AACTCTCTCT GAAGATCAAG CCTAGGAACA TAACAAGGCG GAAAACAGAC 
CGCGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497589.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497652.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X10, mRNA.

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CloneID	OMu29227
Clone ID Related Accession (Same CDS sequence)	XM_006497589.3
Accession Version	XM_006497589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497652.1
CDS	163..3582
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497589.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497589.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGCAG GCCTCCTATG CTGCTTCTTC CTTCTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TATCCGTGTC TAACTCCTTT CTCAACCGGC ACGGCAGCTT GCCTGTGCCC 
TCGGAACCAC CATCTGGGAA GGACGGACAT CCCAGAGATC CTTCGGTCAG CAGTGCATCT 
GGGAAGGACA GCAGAGAAAA TGGGGAAAGG TCCCCAAAGT CACTGGATGG TCTGGAGTCA 
GCGCAGTCAG AAGAGGAAGT GGATGAACTG TCCCTCATTG ACCACAATGA AATTATGGCC 
AGGCTGACAC TCAAGCAAGA GGGTGATGAT GGGCCAGATG TTCGTGGTGG TTCAGGAGAC 
ATCTTACTGG TCCATGCTAC TGAGACAGAC AGAAAAGACC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACCA CCTACAGGAC CTTCATCAGC CCGGAGGAGC TCATCAAGAA GCTGCAGTAC 
CGGTACGAGA AGTTCTCTCC CTTTGCTGAC ACGTTCAAGA AGCGAGTGAG CAAGAACACA 
TTCTTTGTGC TGGTTCGAGT GGTGGATGAG CTCTGCCTGG TGGAGCTGAC AGAGGAGATC 
CTGAAGCTGC TGATGGAGCT GGTCTTCCGC CTAGTGTGCA GCGGAGAGCT CAGCCTGGCC 
AGAGTCCTCC GGAAGAACAT TCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC 
CATTCCGACC AGCCTCTGGC AGCCAGGGGT GTTGCAGCCA GGCCAGGAAC CTTGCATGAT 
TTCCACAGCC ACGAGATAGC TGAGCAGCTG ACACTGCTGG ATGCCGAGCT TTTCTACAAG 
ATAGAGATTC CTGAAGTTTT GCTTTGGGCC AAAGAGCAGA ATGAGGAGAA GAGTCCCAAT 
CTGACCCAGT TCACAGAGCA CTTCAACAAC ATGTCCTACT GGGTGCGGTC CATCATCATG 
CTGCAGGAGA AGGCCCAGGA CCGGGAGAGG CTGCTCCTCA AGTTCATCAA GATCATGAAG 
CACCTGCGCA AGCTCAACAA CTTCAACTCC TACCTGGCCA TCCTCTCAGC ACTAGACTCG 
GCCCCCATCC GCAGACTGGA GTGGCAGCGG CAGACCTCAG AGGGCCTGGC TGAGTACTGC 
ACATTGATTG ACAGCTCATC CTCCTTCCGA GCCTACCGGG CTGCTCTCTC AGAGGTGGAG 
CCCCCATGTA TCCCATACCT AGGTCTGATT CTGCAAGACC TGACCTTCGT TCACCTGGGA 
AACCCAGATT ATATTGACGG GAAAGTAAAC TTCTCCAAGC GGTGGCAACA GTTCAACATA 
TTGGACAGCA TGCGGTGCTT CCAGCAGGCG CACTATGAAA TCCGGAGAAA CGACGACATC 
ATAAATTTCT TCAATGACTT CAGTGACCAC CTGGCCGAGG AAGCCCTGTG GGAACTCTCT 
CTGAAGATCA AGCCTAGGAA CATAACAAGG CGGAAAACAG ACCGCGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497590.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497653.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu29228
Clone ID Related Accession (Same CDS sequence)	XM_006497590.3
Accession Version	XM_006497590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497653.1
CDS	163..3579
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPSEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497590.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497590.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGCC TCCTATGCTG CTTCTTCCTT CTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGGCCTTC ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC 
CTCAGTGTAT CCGTGTCTAA CTCCTTTCTC AACCGGCACG GCAGCTTGCC TGTGCCCTCG 
GAACCACCAT CTGGGAAGGA CGGACATCCC AGAGATCCTT CGGTCAGCAG TGCATCTGGG 
AAGGACAGCA GAGAAAATGG GGAAAGGTCC CCAAAGTCAC TGGATGGTCT GGAGTCAGCG 
CAGTCAGAAG AGGAAGTGGA TGAACTGTCC CTCATTGACC ACAATGAAAT TATGGCCAGG 
CTGACACTCA AGCAAGAGGG TGATGATGGG CCAGATGTTC GTGGTGGTTC AGGAGACATC 
TTACTGGTCC ATGCTACTGA GACAGACAGA AAAGACCTGG TGCTGTACTG TGAAGCCTTC 
CTGACCACCT ACAGGACCTT CATCAGCCCG GAGGAGCTCA TCAAGAAGCT GCAGTACCGG 
TACGAGAAGT TCTCTCCCTT TGCTGACACG TTCAAGAAGC GAGTGAGCAA GAACACATTC 
TTTGTGCTGG TTCGAGTGGT GGATGAGCTC TGCCTGGTGG AGCTGACAGA GGAGATCCTG 
AAGCTGCTGA TGGAGCTGGT CTTCCGCCTA GTGTGCAGCG GAGAGCTCAG CCTGGCCAGA 
GTCCTCCGGA AGAACATTCT GGACAAGGTG GACCAGAAGA AGCTGCTCAG GTGTGCCCAT 
TCCGACCAGC CTCTGGCAGC CAGGGGTGTT GCAGCCAGGC CAGGAACCTT GCATGATTTC 
CACAGCCACG AGATAGCTGA GCAGCTGACA CTGCTGGATG CCGAGCTTTT CTACAAGATA 
GAGATTCCTG AAGTTTTGCT TTGGGCCAAA GAGCAGAATG AGGAGAAGAG TCCCAATCTG 
ACCCAGTTCA CAGAGCACTT CAACAACATG TCCTACTGGG TGCGGTCCAT CATCATGCTG 
CAGGAGAAGG CCCAGGACCG GGAGAGGCTG CTCCTCAAGT TCATCAAGAT CATGAAGCAC 
CTGCGCAAGC TCAACAACTT CAACTCCTAC CTGGCCATCC TCTCAGCACT AGACTCGGCC 
CCCATCCGCA GACTGGAGTG GCAGCGGCAG ACCTCAGAGG GCCTGGCTGA GTACTGCACA 
TTGATTGACA GCTCATCCTC CTTCCGAGCC TACCGGGCTG CTCTCTCAGA GGTGGAGCCC 
CCATGTATCC CATACCTAGG TCTGATTCTG CAAGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGATTATA TTGACGGGAA AGTAAACTTC TCCAAGCGGT GGCAACAGTT CAACATATTG 
GACAGCATGC GGTGCTTCCA GCAGGCGCAC TATGAAATCC GGAGAAACGA CGACATCATA 
AATTTCTTCA ATGACTTCAG TGACCACCTG GCCGAGGAAG CCCTGTGGGA ACTCTCTCTG 
AAGATCAAGC CTAGGAACAT AACAAGGCGG AAAACAGACC GCGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef1 ( XM_006497592.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497655.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X14, mRNA.

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CloneID	OMu29230
Clone ID Related Accession (Same CDS sequence)	XM_006497592.3
Accession Version	XM_006497592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGAA CCACCATCTG GGAAGGACGG ACATCCCAGA 
GATCCTTCGG TCAGCAGTGC ATCTGGGAAG GACAGCAGAG AAAATGGGGA AAGGTCCCCA 
AAGTCACTGG ATGGTCTGGA GTCAGCGCAG TCAGAAGAGG AAGTGGATGA ACTGTCCCTC 
ATTGACCACA ATGAAATTAT GGCCAGGCTG ACACTCAAGC AAGAGGGTGA TGATGGGCCA 
GATGTTCGTG GTGGTTCAGG AGACATCTTA CTGGTCCATG CTACTGAGAC AGACAGAAAA 
GACCTGGTGC TGTACTGTGA AGCCTTCCTG ACCACCTACA GGACCTTCAT CAGCCCGGAG 
GAGCTCATCA AGAAGCTGCA GTACCGGTAC GAGAAGTTCT CTCCCTTTGC TGACACGTTC 
AAGAAGCGAG TGAGCAAGAA CACATTCTTT GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC 
CTGGTGGAGC TGACAGAGGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTAGTG 
TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC CTCCGGAAGA ACATTCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCCATTCC GACCAGCCTC TGGCAGCCAG GGGTGTTGCA 
GCCAGGCCAG GAACCTTGCA TGATTTCCAC AGCCACGAGA TAGCTGAGCA GCTGACACTG 
CTGGATGCCG AGCTTTTCTA CAAGATAGAG ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG 
CAGAATGAGG AGAAGAGTCC CAATCTGACC CAGTTCACAG AGCACTTCAA CAACATGTCC 
TACTGGGTGC GGTCCATCAT CATGCTGCAG GAGAAGGCCC AGGACCGGGA GAGGCTGCTC 
CTCAAGTTCA TCAAGATCAT GAAGCACCTG CGCAAGCTCA ACAACTTCAA CTCCTACCTG 
GCCATCCTCT CAGCACTAGA CTCGGCCCCC ATCCGCAGAC TGGAGTGGCA GCGGCAGACC 
TCAGAGGGCC TGGCTGAGTA CTGCACATTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA TGTATCCCAT ACCTAGGTCT GATTCTGCAA 
GACCTGACCT TCGTTCACCT GGGAAACCCA GATTATATTG ACGGGAAAGT AAACTTCTCC 
AAGCGGTGGC AACAGTTCAA CATATTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GAAATCCGGA GAAACGACGA CATCATAAAT TTCTTCAATG ACTTCAGTGA CCACCTGGCC 
GAGGAAGCCC TGTGGGAACT CTCTCTGAAG ATCAAGCCTA GGAACATAAC AAGGCGGAAA 
ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497655.1
CDS	161..3424
Translation	MGNAVEKQKPLRQSHLCPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLEPPSGKDGHPRDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006497592.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497592.3

ORF Insert Sequence:

ATGGGCAATG CCGTTGAGAA ACAGAAGCCC CTGAGACAAA GCCATCTGTG CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCTCCCAAAA TCAAGAGAAC ACCATCCAAG AAGGGAAAGC CAGCCGAGGT GTCGAAGATT 
CCTGAGAAGC CTGTGAGCAA AGAGGCAAGA GACAGATTTC TACCAGAGGG CTACCCTATC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAGAGGC AGAAGAACCT GTGCTGGCTG GAGGAGAAAG AGAAGGAGGT TGTCAGTGCC 
TTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCCATTG ATAAGAAGGT TCTGGAGATG 
CTCCCGGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GACTGACCCT 
CGGATCCAGC ACAGCTCAGC CCTCTCCTCC TGTTATAGCC GAGTATACCA GAGCCTCGCC 
AACCTTATCC GATGGTCTGA CCAGGTGATG CTAGAGGGCG TGAACTCAGA AGATAAGGAG 
ATGGTGACAA CTGTGAAGGG CGTTATCAAA GCTGTCCTGG ACGGAGTGAA GGAGCTAGTG 
AGGCTAACCA TTGAGAAGCA GGGGCGGCCA TCGCCAACAA GCCCAGTGAA GCCCAGTTCC 
CCAGCCAGCA AGCCTGATGG CCAGCCTGAG CTCCCTCTGA CAGACCGAGA AATGGAGATT 
CTGAACAAGA CGACAAGTGT GTCACCATCT GCTGAACTGC TCCCAGACTC CACCAGTGAA 
GAGGTCGCAC CCCCCAAGCC CCCTTTACCT GGCATCCGGG TGGTTGATAA CAGTCCACCA 
GCATTACCAC CCAAGAAAAG GCAGTCTGCT CCATCCCCCA CTCGGGTGGC TGTGGTAGCC 
CCAATGAGTC GGGCTACCAG TGGCTCCAGT TTGCCTGTTG GAATCAATAG GCAGGACTTT 
GATGTTGAAT GTTACACCCA GAGGCGCCTG TCAGGAGGCA GCCGCTCCTG CGGTGGTGAG 
TCTCCTCGCC TGTCCCCCTG CAGCAGCACA GGCAAGCTCA GCCGCTCAGA CGAGCAGCTG 
TCCTCCCTGG ACAGGGATAG TGGGCAGTGC TCACGGAACA CAAGCTGTGA AACACTAGAT 
CACTACGACC CCGACTATGA ATTCCTCCAG CAAGATCTCT CCAATGCAGA CCAGATCCCT 
CCACAGGCAG CCTGTAACCT CAGCCCTCTG CCGGAGTCCC TGGGGGAATC TGGGCCTCCA 
TTTCTTGGCC ACCCTTTCCA GCTGCCTTTG GGCAGCTGTC TGCAGCAGGA GGGACAGCAG 
ACAGACACTC CACCTGCCCT TCCGGAGAAG AAGCGTAGGA GCGCAGTCTC CCAGACCACG 
GACAGCTCTG GCTGCAGGGT GTCCTATGAG CGACACCCCT CACAGTATGA CAACATCTCA 
GAGGGTGACC TGCAGAACCC AGTCCCAGTC CAGCCTGTGC CCTACCCACC CTTTGCTGCT 
GTCCTGCCCT TTCAGCAGGG AGCTTCCTCT GCCTCTGCTG AGTTTGTGGG TGATTTCAGT 
GTTCCTGAGT TGGCGGGTGA CACAGAGAAG CCACCTCCTC TACCAGAGAA GAAGAACAAG 
CACATGCTGG CCTACATGCA ACTGCTGGAG GACTACTCAG AGCCACAGCC CTCCATGTTC 
TACCAGACAC CGCAGAGTGA GCACATCTAC CAGCAGAAGA ACAAGATGCT CATGGAGGTG 
TACGGCTTCA GCGAGTCCTT CTGCGGTAGT GATTCCACGC AGGAGCTGGC CCCTCCACCC 
GCTCTGCCCC CCAAGCAACG GCAGCTGGAA CCACCATCTG GGAAGGACGG ACATCCCAGA 
GATCCTTCGG TCAGCAGTGC ATCTGGGAAG GACAGCAGAG AAAATGGGGA AAGGTCCCCA 
AAGTCACTGG ATGGTCTGGA GTCAGCGCAG TCAGAAGAGG AAGTGGATGA ACTGTCCCTC 
ATTGACCACA ATGAAATTAT GGCCAGGCTG ACACTCAAGC AAGAGGGTGA TGATGGGCCA 
GATGTTCGTG GTGGTTCAGG AGACATCTTA CTGGTCCATG CTACTGAGAC AGACAGAAAA 
GACCTGGTGC TGTACTGTGA AGCCTTCCTG ACCACCTACA GGACCTTCAT CAGCCCGGAG 
GAGCTCATCA AGAAGCTGCA GTACCGGTAC GAGAAGTTCT CTCCCTTTGC TGACACGTTC 
AAGAAGCGAG TGAGCAAGAA CACATTCTTT GTGCTGGTTC GAGTGGTGGA TGAGCTCTGC 
CTGGTGGAGC TGACAGAGGA GATCCTGAAG CTGCTGATGG AGCTGGTCTT CCGCCTAGTG 
TGCAGCGGAG AGCTCAGCCT GGCCAGAGTC CTCCGGAAGA ACATTCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCCATTCC GACCAGCCTC TGGCAGCCAG GGGTGTTGCA 
GCCAGGCCAG GAACCTTGCA TGATTTCCAC AGCCACGAGA TAGCTGAGCA GCTGACACTG 
CTGGATGCCG AGCTTTTCTA CAAGATAGAG ATTCCTGAAG TTTTGCTTTG GGCCAAAGAG 
CAGAATGAGG AGAAGAGTCC CAATCTGACC CAGTTCACAG AGCACTTCAA CAACATGTCC 
TACTGGGTGC GGTCCATCAT CATGCTGCAG GAGAAGGCCC AGGACCGGGA GAGGCTGCTC 
CTCAAGTTCA TCAAGATCAT GAAGCACCTG CGCAAGCTCA ACAACTTCAA CTCCTACCTG 
GCCATCCTCT CAGCACTAGA CTCGGCCCCC ATCCGCAGAC TGGAGTGGCA GCGGCAGACC 
TCAGAGGGCC TGGCTGAGTA CTGCACATTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCTGCTC TCTCAGAGGT GGAGCCCCCA TGTATCCCAT ACCTAGGTCT GATTCTGCAA 
GACCTGACCT TCGTTCACCT GGGAAACCCA GATTATATTG ACGGGAAAGT AAACTTCTCC 
AAGCGGTGGC AACAGTTCAA CATATTGGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GAAATCCGGA GAAACGACGA CATCATAAAT TTCTTCAATG ACTTCAGTGA CCACCTGGCC 
GAGGAAGCCC TGTGGGAACT CTCTCTGAAG ATCAAGCCTA GGAACATAAC AAGGCGGAAA 
ACAGACCGCG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development.
PloS one11(4)e0154174(2016)
Shah B,Lutter D,Bochenek ML,Kato K,Tsytsyura Y,Glyvuk N,Sakakibara A,Klingauf J,Adams RH,P?schel AW

C3G (RapGEF1), a regulator of actin dynamics promotes survival and myogenic differentiation of mouse mesenchymal cells.
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Rapgef1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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