RAPGEF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF1
Entrez Gene ID	2889
Full Name	Rap guanine nucleotide exchange factor 1
Synonyms	C3G,GRF2
General protein information	Preferred Names Rap guanine nucleotide exchange factor 1 Names rap guanine nucleotide exchange factor 1 CRK SH3-binding GNRP Rap guanine nucleotide exchange factor (GEF) 1 guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.13
Summary	This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	600303

mRNA and Protein(s)

mRNA	Protein	Name
XM_017014638.1	XP_016870127.1	rap guanine nucleotide exchange factor 1 isoform X16
XM_017014640.1	XP_016870129.1	rap guanine nucleotide exchange factor 1 isoform X23
XM_011518581.2	XP_011516883.1	rap guanine nucleotide exchange factor 1 isoform X26
NM_001304275.1	NP_001291204.1	rap guanine nucleotide exchange factor 1 isoform c
XM_011518574.3	XP_011516876.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_017014634.1	XP_016870123.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_006717074.3	XP_006717137.1	rap guanine nucleotide exchange factor 1 isoform X22
XM_017014640.2	XP_016870129.1	rap guanine nucleotide exchange factor 1 isoform X24
XM_017014641.2	XP_016870130.1	rap guanine nucleotide exchange factor 1 isoform X25
XM_011518580.3	XP_011516882.1	rap guanine nucleotide exchange factor 1 isoform X26
XM_011518579.2	XP_011516881.1	rap guanine nucleotide exchange factor 1 isoform X21
XM_011518575.2	XP_011516877.1	rap guanine nucleotide exchange factor 1 isoform X10
NM_005312.3	NP_005303.2	rap guanine nucleotide exchange factor 1 isoform a
XM_011518571.2	XP_011516873.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_011518573.3	XP_011516875.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_011518575.3	XP_011516877.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_017014636.1	XP_016870125.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_011518570.2	XP_011516872.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_011518576.2	XP_011516878.1	rap guanine nucleotide exchange factor 1 isoform X18
XM_011518570.3	XP_011516872.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_011518572.2	XP_011516874.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_005272186.4	XP_005272243.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_017014633.1	XP_016870122.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_017014636.2	XP_016870125.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_005272191.3	XP_005272248.1	rap guanine nucleotide exchange factor 1 isoform X14
XM_017014637.2	XP_016870126.1	rap guanine nucleotide exchange factor 1 isoform X15
XM_017014639.2	XP_016870128.1	rap guanine nucleotide exchange factor 1 isoform X17
XM_011518577.3	XP_011516879.1	rap guanine nucleotide exchange factor 1 isoform X19
XM_011518580.2	XP_011516882.1	rap guanine nucleotide exchange factor 1 isoform X25
XM_011518569.2	XP_011516871.1	rap guanine nucleotide exchange factor 1 isoform X1
NM_198679.1	NP_941372.1	rap guanine nucleotide exchange factor 1 isoform b
XM_017014635.2	XP_016870124.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_017014638.2	XP_016870127.1	rap guanine nucleotide exchange factor 1 isoform X16
XM_011518581.3	XP_011516883.1	rap guanine nucleotide exchange factor 1 isoform X27
XM_011518582.3	XP_011516884.1	rap guanine nucleotide exchange factor 1 isoform X28
XM_011518578.2	XP_011516880.1	rap guanine nucleotide exchange factor 1 isoform X20
XM_017014641.1	XP_016870130.1	rap guanine nucleotide exchange factor 1 isoform X24
XM_011518582.2	XP_011516884.1	rap guanine nucleotide exchange factor 1 isoform X27
XM_011518574.2	XP_011516876.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_017014635.1	XP_016870124.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_017014637.1	XP_016870126.1	rap guanine nucleotide exchange factor 1 isoform X15
XM_017014639.1	XP_016870128.1	rap guanine nucleotide exchange factor 1 isoform X17
XM_011518577.2	XP_011516879.1	rap guanine nucleotide exchange factor 1 isoform X19
XM_011518573.2	XP_011516875.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_011518569.3	XP_011516871.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_006717067.3	XP_006717130.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_006717072.3	XP_006717135.1	rap guanine nucleotide exchange factor 1 isoform X13
XM_011518576.3	XP_011516878.1	rap guanine nucleotide exchange factor 1 isoform X18
XM_011518578.3	XP_011516880.1	rap guanine nucleotide exchange factor 1 isoform X20
XM_011518579.3	XP_011516881.1	rap guanine nucleotide exchange factor 1 isoform X21
XM_024447521.1	XP_024303289.1	rap guanine nucleotide exchange factor 1 isoform X23

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Gene Ontology
Bibliography

Related articles in PubMed

C3G knock-down enhances migration and invasion by increasing Rap1-mediated p38? activation, while it impairs tumor growth through p38?-independent mechanisms.
Priego N, Arechederra M, Sequera C, Bragado P, V?zquez-Carballo A, Guti?rrez-Uzquiza ?, Mart?n-Granado V, Ventura JJ, Kazanietz MG, Guerrero C, Porras A
Oncotarget7(29)45060-45078(2016 Jul)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

The C3G/Rap1 pathway promotes secretion of MMP-2 and MMP-9 and is involved in serous ovarian cancer metastasis.
Che YL, Luo SJ, Li G, Cheng M, Gao YM, Li XM, Dai JM, He H, Wang J, Peng HJ, Zhang Y, Li WY, Wang H, Liu B, Linghu H
Cancer letters359(2)241-9(2015 Apr)

C3G transgenic mouse models with specific expression in platelets reveal a new role for C3G in platelet clotting through its GEF activity.
Guti?rrez-Herrero S, Maia V, Guti?rrez-Berzal J, Calzada N, Sanz M, Gonz?lez-Manch?n C, Pericacho M, Ortiz-Rivero S, Gonz?lez-Porras JR, Arechederra M, Porras A, Guerrero C
Biochimica et biophysica acta1823(8)1366-77(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C3G/RAP1 activity is involved in the metastatic spread of epithelial ovarian cancer.
Title: The C3G/Rap1 pathway promotes secretion of MMP-2 and MMP-9 and is involved in serous ovarian cancer metastasis.

C3G plays an important role in platelet clotting through a mechanism involving its GEF activity and suggest that it might be also involved in neutrophil development.
Title: C3G transgenic mouse models with specific expression in platelets reveal a new role for C3G in platelet clotting through its GEF activity.

Lyn controls spatial activation of Rap1 by recruiting the CrkL-C3G protein complex to the leading edge
Title: The non-receptor tyrosine kinase Lyn controls neutrophil adhesion by recruiting the CrkL-C3G complex and activating Rap1 at the leading edge.

found somatic demethylation of a relaxed-criterion CpG island (CGI-B) located in the first intron of RAPGEF1 in 40% of colon cancers and 8% of gastric cancers relative to their matching normal tissues that were always methylated
Title: Frequent somatic demethylation of RAPGEF1/C3G intronic sequences in gastrointestinal and gynecological cancer.

The possibility of cellular phospho-C3G (pC3G) being a substrate of the intracellular T-cell protein tyrosine phosphatase TC-PTP (PTPN2) using the human neuroblastoma cell line, was studied.
Title: TC-PTP dephosphorylates the guanine nucleotide exchange factor C3G (RapGEF1) and negatively regulates differentiation of human neuroblastoma cells.

The following RAPGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu58067	XM_011518580.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu58068	XM_011518581.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu58069	XM_011518582.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu98619	XM_017014641.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu02636	NM_198679.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu106137	XM_024447521.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu98612	XM_017014633.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98613	XM_017014635.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98613	XM_017014634.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98614	XM_017014636.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98615	XM_017014637.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98616	XM_017014638.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98617	XM_017014639.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98618	XM_017014640.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu04877	NM_005312.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$762.30 $1089.00
OHu43223	NM_001304275.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu43212	XM_006717067.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43215	XM_005272186.4 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43219	XM_006717072.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43220	XM_005272191.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43222	XM_006717074.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58056	XM_011518569.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58057	XM_011518570.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58058	XM_011518571.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58059	XM_011518572.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58060	XM_011518573.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58061	XM_011518574.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58062	XM_011518575.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58063	XM_011518576.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58064	XM_011518577.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58065	XM_011518578.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58066	XM_011518579.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu98618	XM_017014640.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu58062	XM_011518575.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58057	XM_011518570.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu98614	XM_017014636.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu58067	XM_011518580.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu98616	XM_017014638.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu58068	XM_011518581.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu98619	XM_017014641.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu58069	XM_011518582.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu58061	XM_011518574.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu98613	XM_017014635.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98615	XM_017014637.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu98617	XM_017014639.1	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu58064	XM_011518577.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58060	XM_011518573.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58056	XM_011518569.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu58063	XM_011518576.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58065	XM_011518578.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58066	XM_011518579.2	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF1 ( XM_011518580.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516882.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu58067
Clone ID Related Accession (Same CDS sequence)	XM_011518580.3 , XM_011518580.2
Accession Version	XM_011518580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGCGGA ATCACTTCCG TGTACAGATG GAACATCCAG 
AGGCAGCAGC GACTGTCCTA TGGCCACACA GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516882.1
CDS	177..2798
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSESGITSVYRWNIQRQQRLSYGHTASQ

Target ORF information:

RefSeq Version	XM_011518580.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518580.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGCGGA ATCACTTCCG TGTACAGATG GAACATCCAG 
AGGCAGCAGC GACTGTCCTA TGGCCACACA GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518580.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516882.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu58067
Clone ID Related Accession (Same CDS sequence)	XM_011518580.3 , XM_011518580.2
Accession Version	XM_011518580.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGCGGA ATCACTTCCG TGTACAGATG GAACATCCAG 
AGGCAGCAGC GACTGTCCTA TGGCCACACA GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516882.1
CDS	176..2797
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSESGITSVYRWNIQRQQRLSYGHTASQ

Target ORF information:

RefSeq Version	XM_011518580.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518580.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGCGGA ATCACTTCCG TGTACAGATG GAACATCCAG 
AGGCAGCAGC GACTGTCCTA TGGCCACACA GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518581.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516883.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu58068
Clone ID Related Accession (Same CDS sequence)	XM_011518581.2 , XM_011518581.3
Accession Version	XM_011518581.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGCGGA 
ATCACTTCCG TGTACAGATG GAACATCCAG AGGCAGCAGC GACTGTCCTA TGGCCACACA 
GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516883.1
CDS	176..2287
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSESGITSVYRWNIQRQQRLSYGHTASQ

Target ORF information:

RefSeq Version	XM_011518581.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518581.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGCGGA 
ATCACTTCCG TGTACAGATG GAACATCCAG AGGCAGCAGC GACTGTCCTA TGGCCACACA 
GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518581.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516883.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

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CloneID	OHu58068
Clone ID Related Accession (Same CDS sequence)	XM_011518581.2 , XM_011518581.3
Accession Version	XM_011518581.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGCGGA 
ATCACTTCCG TGTACAGATG GAACATCCAG AGGCAGCAGC GACTGTCCTA TGGCCACACA 
GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516883.1
CDS	177..2288
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSESGITSVYRWNIQRQQRLSYGHTASQ

Target ORF information:

RefSeq Version	XM_011518581.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518581.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGCGGA 
ATCACTTCCG TGTACAGATG GAACATCCAG AGGCAGCAGC GACTGTCCTA TGGCCACACA 
GCCAGTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518582.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516884.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OHu58069
Clone ID Related Accession (Same CDS sequence)	XM_011518582.3 , XM_011518582.2
Accession Version	XM_011518582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGACC TCCCAGAAAG GAGGGACTCA ATCAAAGCAC 
AGGACATGGT CTGTGCACAG CCATCTGTGG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516884.1
CDS	177..2192
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLTSQKGGTQSKHRTWSVHSHLWT

Target ORF information:

RefSeq Version	XM_011518582.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518582.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGACC TCCCAGAAAG GAGGGACTCA ATCAAAGCAC 
AGGACATGGT CTGTGCACAG CCATCTGTGG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518582.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516884.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

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CloneID	OHu58069
Clone ID Related Accession (Same CDS sequence)	XM_011518582.3 , XM_011518582.2
Accession Version	XM_011518582.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGACC TCCCAGAAAG GAGGGACTCA ATCAAAGCAC 
AGGACATGGT CTGTGCACAG CCATCTGTGG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516884.1
CDS	176..2191
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLTSQKGGTQSKHRTWSVHSHLWT

Target ORF information:

RefSeq Version	XM_011518582.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518582.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGACC TCCCAGAAAG GAGGGACTCA ATCAAAGCAC 
AGGACATGGT CTGTGCACAG CCATCTGTGG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014641.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870130.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

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CloneID	OHu98619
Clone ID Related Accession (Same CDS sequence)	XM_017014641.2 , XM_017014641.1
Accession Version	XM_017014641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGCGAGGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870130.1
CDS	177..2921
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKARG

Target ORF information:

RefSeq Version	XM_017014641.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014641.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGCGAGGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014641.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870130.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu98619
Clone ID Related Accession (Same CDS sequence)	XM_017014641.2 , XM_017014641.1
Accession Version	XM_017014641.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGCGAGGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870130.1
CDS	176..2920
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKARG

Target ORF information:

RefSeq Version	XM_017014641.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014641.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGCGAGGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( NM_198679.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_941372.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 2, mRNA.

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CloneID	OHu02636
Clone ID Related Accession (Same CDS sequence)	NM_198679.1
Accession Version	NM_198679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG546427.1, BG429001.1, BC041710.1, BQ054243.1, BQ772947.1 and AJ420417.1. Transcript Variant: This variant (2) encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAATG CTATTGAAAA ACAGAAGCCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTCTCT TCCTGCTATA GCCGAGTGTA CCAAAGCCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACTGTGAA GGGGGTCATC AAGGCTGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCATCGAGAA GCAGGGACGT CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT 
TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAG 
ATCCTAAACA AGACGACTGG GATGTCACAG TCAACTGAGC TCCTCCCAGA TGCCACGGAT 
GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCGCCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTC TTGGCCCTCC TTTCCAGCTG CCTCTTGGCG GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGATACG CCACCTGCTC TCCCCGAGAA GAAGCGCAGG 
AGCGCAGCCT CCCAGACGGC GGACGGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTATG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CAGCCCCGAT CCCATCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTGCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAG TCAACCGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGTTGCTGGA GGACTACTCG 
GAGCCGCAGC CCTCTATGTT CTACCAGACG CCACAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGTGGGGT GGACTCCGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_941372.1
CDS	160..3447
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_198679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198679.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAGCCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTCTCT TCCTGCTATA GCCGAGTGTA CCAAAGCCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GCGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACTGTGAA GGGGGTCATC AAGGCTGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCATCGAGAA GCAGGGACGT CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT 
TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAG 
ATCCTAAACA AGACGACTGG GATGTCACAG TCAACTGAGC TCCTCCCAGA TGCCACGGAT 
GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCGCCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTC TTGGCCCTCC TTTCCAGCTG CCTCTTGGCG GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGATACG CCACCTGCTC TCCCCGAGAA GAAGCGCAGG 
AGCGCAGCCT CCCAGACGGC GGACGGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTATG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CAGCCCCGAT CCCATCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTGCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAG TCAACCGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGTTGCTGGA GGACTACTCG 
GAGCCGCAGC CCTCTATGTT CTACCAGACG CCACAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGTGGGGT GGACTCCGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_024447521.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303289.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu106137
Clone ID Related Accession (Same CDS sequence)	XM_024447521.1
Accession Version	XM_024447521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303289.1
CDS	193..3474
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_024447521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447521.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014633.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870122.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

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CloneID	OHu98612
Clone ID Related Accession (Same CDS sequence)	XM_017014633.1
Accession Version	XM_017014633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAGGCAACAG ACAGATTTCT ACCAGAGGGC 
TACCCTCTCC CCTTGGATCT GGAGCAGCAG GCAGTAGAAT TTATGTCCAC CAGTGCTGTG 
GCTTCCAGGT CTCAAAGGCA GAAGAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTCTCTTCCT GCTATAGCCG AGTGTACCAA 
AGCCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC TGTGAAGGGG GTCATCAAGG CTGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCAT CGAGAAGCAG GGACGTCCGT CTCCGACGAG CCCCGTGAAG 
CCCAGTTCCC CTGCCAGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAGATCC TAAACAAGAC GACTGGGATG TCACAGTCAA CTGAGCTCCT CCCAGATGCC 
ACGGATGAAG AGGTCGCGCC CCCCAAGCCT CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CGCCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGATGTTGA CTGTTACGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTCTTGG CCCTCCTTTC CAGCTGCCTC TTGGCGGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GATACGCCAC CTGCTCTCCC CGAGAAGAAG 
CGCAGGAGCG CAGCCTCCCA GACGGCGGAC GGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTATGACAA CATCTCTGGG GAGGACCTGC AGAGCACAGC CCCGATCCCA 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTGCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAGTCAA CCGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGTT GCTGGAGGAC 
TACTCGGAGC CGCAGCCCTC TATGTTCTAC CAGACGCCAC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG TGGGGTGGAC 
TCCGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCCACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACCA CCAAGCTTCC GTTCCGCCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGCCCACCAG TCTCAGAGCT CTGACTTAGC CGTGCCAACC 
ATGGCCGGTC CACCTCCCAG CACCGTGGAC GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC 
TTTCATGGGA ATACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAC 
GCCAGTGAGG AAGCTGGTGA GGGTGAATAT GTCAATCTGT ATTCCTCTGG CCAGAGCAGC 
GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870122.1
CDS	262..4005
Translation	MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014633.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014633.1

ORF Insert Sequence:

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAGGCAACAG ACAGATTTCT ACCAGAGGGC 
TACCCTCTCC CCTTGGATCT GGAGCAGCAG GCAGTAGAAT TTATGTCCAC CAGTGCTGTG 
GCTTCCAGGT CTCAAAGGCA GAAGAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTCTCTTCCT GCTATAGCCG AGTGTACCAA 
AGCCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC TGTGAAGGGG GTCATCAAGG CTGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCAT CGAGAAGCAG GGACGTCCGT CTCCGACGAG CCCCGTGAAG 
CCCAGTTCCC CTGCCAGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAGATCC TAAACAAGAC GACTGGGATG TCACAGTCAA CTGAGCTCCT CCCAGATGCC 
ACGGATGAAG AGGTCGCGCC CCCCAAGCCT CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CGCCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGATGTTGA CTGTTACGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTCTTGG CCCTCCTTTC CAGCTGCCTC TTGGCGGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GATACGCCAC CTGCTCTCCC CGAGAAGAAG 
CGCAGGAGCG CAGCCTCCCA GACGGCGGAC GGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTATGACAA CATCTCTGGG GAGGACCTGC AGAGCACAGC CCCGATCCCA 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTGCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAGTCAA CCGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGTT GCTGGAGGAC 
TACTCGGAGC CGCAGCCCTC TATGTTCTAC CAGACGCCAC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG TGGGGTGGAC 
TCCGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCCACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACCA CCAAGCTTCC GTTCCGCCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGCCCACCAG TCTCAGAGCT CTGACTTAGC CGTGCCAACC 
ATGGCCGGTC CACCTCCCAG CACCGTGGAC GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC 
TTTCATGGGA ATACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAC 
GCCAGTGAGG AAGCTGGTGA GGGTGAATAT GTCAATCTGT ATTCCTCTGG CCAGAGCAGC 
GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014634.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870123.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

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CloneID	OHu98613
Clone ID Related Accession (Same CDS sequence)	XM_017014634.1 , XM_017014635.2 , XM_017014635.1
Accession Version	XM_017014634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870123.1
CDS	131..3856
Translation	MKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014634.1

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014635.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870124.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu98613
Clone ID Related Accession (Same CDS sequence)	XM_017014634.1 , XM_017014635.2 , XM_017014635.1
Accession Version	XM_017014635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870124.1
CDS	189..3914
Translation	MKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014635.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014635.2

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014635.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870124.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

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CloneID	OHu98613
Clone ID Related Accession (Same CDS sequence)	XM_017014634.1 , XM_017014635.2 , XM_017014635.1
Accession Version	XM_017014635.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870124.1
CDS	185..3910
Translation	MKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014635.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014635.1

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA ATCCAGGTTT 
CCTCTCTTCC AGGAGGCAAC AGACAGATTT CTACCAGAGG GCTACCCTCT CCCCTTGGAT 
CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG TGGCTTCCAG GTCTCAAAGG 
CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG TTGTCAGTGC CCTGCGCTAC 
TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG TACTGGAGAT GCTTCCAGGG 
TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC AGAACGATCC TCGAATTCAG 
CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC AAAGCCTCGC CAACCTCATT 
CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG AAGACAAGGA GATGGTGACG 
ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA AGGAGCTGGT CAGGCTCACC 
ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA AGCCCAGTTC CCCTGCCAGC 
AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG AGGTAGAGAT CCTAAACAAG 
ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG CCACGGATGA AGAGGTCGCG 
CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA ATAGTCCTCC ACCAGCATTG 
CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG TGGCTGTGGT GGCCCCCATG 
AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA ATAGGCAGGA TTTTGATGTT 
GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT CATATGGTGG AGAGTCGCCC 
CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT CAGACGAGCA GCTGTCCTCT 
CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT GTGAAACACT AGACCACTAT 
GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG CAGACCAGAT ACCTCAGCAG 
ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG AGTCTGGGTC TCCATTTCTT 
GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC CAGACGGACC TCTGGCCCCA 
GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA AGCGCAGGAG CGCAGCCTCC 
CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC GGCATCCCTC GCAGTATGAC 
AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC CATCCGTCCC CTACGCGCCC 
TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG CCCCTGTCGA ATTTGTGGGT 
GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC CACCTCCTCT ACCAGAGAAG 
AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG ACTACTCGGA GCCGCAGCCC 
TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC AGCAGAAGAA CAAGCTCCTC 
ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG ACTCCGTGCA GGAGCTGGCC 
CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGCAGG CCTCCTGTGC TGCTTCTTCC 
TTCTCCTCTG TCTCCCACTG TGTCCAGCAA ACTAAAGTGG CCTTCACCCC CGAGGACGGC 
AGTGCCGCTC AGGGCCTCAG TGTGTCCGTC TCTAACTCCT TTCTCAGCCG GCACGGCAGC 
TTGCCCGTGC CCTCGTATAA GTCTGTGTTT AGGTCCTACT CCCAGGATTT CGTGCCTCAC 
CACCAAGCTT CCGTTCCGCC TTTCCTTCCG CCTACCTCCT CTTCCTCTCC ACATTTCCCA 
CCTGCCCACC AGTCTCAGAG CTCTGACTTA GCCGTGCCAA CCATGGCCGG TCCACCTCCC 
AGCACCGTGG ACGGGCCTCT CTCGGCTTCT CAGGAGAGCA GCTTTCATGG GAATACTGTC 
TGCCTTCCTT CCGAAACCTC TTTCACTGAC TCGAGTGAAA ACGCCAGTGA GGAAGCTGGT 
GAGGGTGAAT ATGTCAATCT GTATTCCTCT GGCCAGAGCA GCGAGGAGCT GGCTCCCTCT 
CGAGGAGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014636.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870125.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

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CloneID	OHu98614
Clone ID Related Accession (Same CDS sequence)	XM_017014636.1 , XM_017014636.2
Accession Version	XM_017014636.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870125.1
CDS	180..3881
Translation	MKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014636.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014636.1

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014636.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870125.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu98614
Clone ID Related Accession (Same CDS sequence)	XM_017014636.1 , XM_017014636.2
Accession Version	XM_017014636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870125.1
CDS	181..3882
Translation	MKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014636.2

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
AAACCAGCTG AGGTGTCCGT AAAGATTCCA GAGAAGCCTG TGAACAAAGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014637.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870126.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

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CloneID	OHu98615
Clone ID Related Accession (Same CDS sequence)	XM_017014637.2 , XM_017014637.1
Accession Version	XM_017014637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCCT CTCGAGGAGA GCCACCGGCT GGGAAAGACG GACATCCCAG AGATCCCTCA 
GCGGTCAGCG GCGTCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATTGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGACGTC 
CGCGGAGGAT CTGGGGACAT CTTACTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCATT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATATGAGAAA TTCTCTCCCT TTGCCGACAC ATTCAAGAAG 
CGCGTCAGCA AGAACACGTT CTTCGTGCTG GTACGGGTGG TGGATGAGCT CTGCCTGGTG 
GAGTTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAT 
GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTACTCA GGTGTGCCAC CTCCAGCCAG CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATAGCGG AGCAGCTAAC GCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAGAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCGGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACTTCAGAG 
GGCCTGGCCG AGTACTGCAC ACTGATCGAC AGCTCGTCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGACGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGCTGCTTCC AGCAGGCGCA CTATGACATG 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTGTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870126.1
CDS	177..3854
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014637.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014637.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCCT CTCGAGGAGA GCCACCGGCT GGGAAAGACG GACATCCCAG AGATCCCTCA 
GCGGTCAGCG GCGTCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATTGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGACGTC 
CGCGGAGGAT CTGGGGACAT CTTACTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCATT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATATGAGAAA TTCTCTCCCT TTGCCGACAC ATTCAAGAAG 
CGCGTCAGCA AGAACACGTT CTTCGTGCTG GTACGGGTGG TGGATGAGCT CTGCCTGGTG 
GAGTTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAT 
GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTACTCA GGTGTGCCAC CTCCAGCCAG CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATAGCGG AGCAGCTAAC GCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAGAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCGGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACTTCAGAG 
GGCCTGGCCG AGTACTGCAC ACTGATCGAC AGCTCGTCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGACGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGCTGCTTCC AGCAGGCGCA CTATGACATG 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTGTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014637.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870126.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu98615
Clone ID Related Accession (Same CDS sequence)	XM_017014637.2 , XM_017014637.1
Accession Version	XM_017014637.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCCT CTCGAGGAGA GCCACCGGCT GGGAAAGACG GACATCCCAG AGATCCCTCA 
GCGGTCAGCG GCGTCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATTGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGACGTC 
CGCGGAGGAT CTGGGGACAT CTTACTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCATT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATATGAGAAA TTCTCTCCCT TTGCCGACAC ATTCAAGAAG 
CGCGTCAGCA AGAACACGTT CTTCGTGCTG GTACGGGTGG TGGATGAGCT CTGCCTGGTG 
GAGTTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAT 
GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTACTCA GGTGTGCCAC CTCCAGCCAG CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATAGCGG AGCAGCTAAC GCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAGAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCGGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACTTCAGAG 
GGCCTGGCCG AGTACTGCAC ACTGATCGAC AGCTCGTCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGACGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGCTGCTTCC AGCAGGCGCA CTATGACATG 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTGTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870126.1
CDS	176..3853
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014637.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014637.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCCT CTCGAGGAGA GCCACCGGCT GGGAAAGACG GACATCCCAG AGATCCCTCA 
GCGGTCAGCG GCGTCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATTGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGACGTC 
CGCGGAGGAT CTGGGGACAT CTTACTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCATT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATATGAGAAA TTCTCTCCCT TTGCCGACAC ATTCAAGAAG 
CGCGTCAGCA AGAACACGTT CTTCGTGCTG GTACGGGTGG TGGATGAGCT CTGCCTGGTG 
GAGTTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAT 
GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTACTCA GGTGTGCCAC CTCCAGCCAG CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATAGCGG AGCAGCTAAC GCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAGAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTGAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCGGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACTTCAGAG 
GGCCTGGCCG AGTACTGCAC ACTGATCGAC AGCTCGTCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGACGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGCTGCTTCC AGCAGGCGCA CTATGACATG 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTGTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014638.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870127.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu98616
Clone ID Related Accession (Same CDS sequence)	XM_017014638.1 , XM_017014638.2
Accession Version	XM_017014638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870127.1
CDS	176..3760
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014638.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870127.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

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CloneID	OHu98616
Clone ID Related Accession (Same CDS sequence)	XM_017014638.1 , XM_017014638.2
Accession Version	XM_017014638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870127.1
CDS	177..3761
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014638.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AACCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014639.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870128.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

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CloneID	OHu98617
Clone ID Related Accession (Same CDS sequence)	XM_017014639.2 , XM_017014639.1
Accession Version	XM_017014639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870128.1
CDS	177..3755
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014639.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014639.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870128.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

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CloneID	OHu98617
Clone ID Related Accession (Same CDS sequence)	XM_017014639.2 , XM_017014639.1
Accession Version	XM_017014639.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870128.1
CDS	176..3754
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_017014639.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014639.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014640.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870129.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

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CloneID	OHu98618
Clone ID Related Accession (Same CDS sequence)	XM_017014640.1 , XM_017014640.2
Accession Version	XM_017014640.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGGCATGG CCCAGTGCTC ACCAGCGAGT 
CCCACGAAGT TGTCATGGCT ACTGGCAATG ATGCACCCGC AAAGGCCAGT TGACTTGGGC 
CCCTTCTTCC AGAAGCAGGT ATGGGTCTAT GCTGGTGCCA TCTCTGCTTC CTTCCCCAAA 
CTCTTTGAAC AAAAATCCAA GCTTGACAGT GAACTGGGTG GGTGTGGGGG CCGAGGAATG 
AGCCTGAGCA CAGTGCGGAT GGGCCCTGCT GTGATTCCCA GCCCGGAGCC TCCTTCACCT 
GCCAGCCCCA CCCCACGCAG CCCCCAGTGC AAGAGCAGGC GTGGGTTTTC CTCTCGTTTG 
ATGCAAGAGG GGATGGGGCA AGGGTGGGGG CAGGAGCCAA GCCAGCATGA CTCAGCTGGA 
GATGTGGGTT CCTCGAGATT CCTCTACCCA GCAGCTCGGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870129.1
CDS	176..3340
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKGMAQCSPASPTKLSWLLAMMHPQRPVDLGPFFQKQVWVYAGAISASFPKLFEQKSKLDSELGGCGGRGMSLSTVRMGPAVIPSPEPPSPASPTPRSPQCKSRRGFSSRLMQEGMGQGWGQEPSQHDSAGDVGSSRFLYPAARE

Target ORF information:

RefSeq Version	XM_017014640.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014640.1

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGGCATGG CCCAGTGCTC ACCAGCGAGT 
CCCACGAAGT TGTCATGGCT ACTGGCAATG ATGCACCCGC AAAGGCCAGT TGACTTGGGC 
CCCTTCTTCC AGAAGCAGGT ATGGGTCTAT GCTGGTGCCA TCTCTGCTTC CTTCCCCAAA 
CTCTTTGAAC AAAAATCCAA GCTTGACAGT GAACTGGGTG GGTGTGGGGG CCGAGGAATG 
AGCCTGAGCA CAGTGCGGAT GGGCCCTGCT GTGATTCCCA GCCCGGAGCC TCCTTCACCT 
GCCAGCCCCA CCCCACGCAG CCCCCAGTGC AAGAGCAGGC GTGGGTTTTC CTCTCGTTTG 
ATGCAAGAGG GGATGGGGCA AGGGTGGGGG CAGGAGCCAA GCCAGCATGA CTCAGCTGGA 
GATGTGGGTT CCTCGAGATT CCTCTACCCA GCAGCTCGGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_017014640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870129.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu98618
Clone ID Related Accession (Same CDS sequence)	XM_017014640.1 , XM_017014640.2
Accession Version	XM_017014640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGGCATGG CCCAGTGCTC ACCAGCGAGT 
CCCACGAAGT TGTCATGGCT ACTGGCAATG ATGCACCCGC AAAGGCCAGT TGACTTGGGC 
CCCTTCTTCC AGAAGCAGGT ATGGGTCTAT GCTGGTGCCA TCTCTGCTTC CTTCCCCAAA 
CTCTTTGAAC AAAAATCCAA GCTTGACAGT GAACTGGGTG GGTGTGGGGG CCGAGGAATG 
AGCCTGAGCA CAGTGCGGAT GGGCCCTGCT GTGATTCCCA GCCCGGAGCC TCCTTCACCT 
GCCAGCCCCA CCCCACGCAG CCCCCAGTGC AAGAGCAGGC GTGGGTTTTC CTCTCGTTTG 
ATGCAAGAGG GGATGGGGCA AGGGTGGGGG CAGGAGCCAA GCCAGCATGA CTCAGCTGGA 
GATGTGGGTT CCTCGAGATT CCTCTACCCA GCAGCTCGGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870129.1
CDS	177..3341
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKGMAQCSPASPTKLSWLLAMMHPQRPVDLGPFFQKQVWVYAGAISASFPKLFEQKSKLDSELGGCGGRGMSLSTVRMGPAVIPSPEPPSPASPTPRSPQCKSRRGFSSRLMQEGMGQGWGQEPSQHDSAGDVGSSRFLYPAARE

Target ORF information:

RefSeq Version	XM_017014640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014640.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGGCATGG CCCAGTGCTC ACCAGCGAGT 
CCCACGAAGT TGTCATGGCT ACTGGCAATG ATGCACCCGC AAAGGCCAGT TGACTTGGGC 
CCCTTCTTCC AGAAGCAGGT ATGGGTCTAT GCTGGTGCCA TCTCTGCTTC CTTCCCCAAA 
CTCTTTGAAC AAAAATCCAA GCTTGACAGT GAACTGGGTG GGTGTGGGGG CCGAGGAATG 
AGCCTGAGCA CAGTGCGGAT GGGCCCTGCT GTGATTCCCA GCCCGGAGCC TCCTTCACCT 
GCCAGCCCCA CCCCACGCAG CCCCCAGTGC AAGAGCAGGC GTGGGTTTTC CTCTCGTTTG 
ATGCAAGAGG GGATGGGGCA AGGGTGGGGG CAGGAGCCAA GCCAGCATGA CTCAGCTGGA 
GATGTGGGTT CCTCGAGATT CCTCTACCCA GCAGCTCGGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( NM_005312.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005303.2 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 1, mRNA.

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CloneID	OHu04877
Clone ID Related Accession (Same CDS sequence)	NM_005312.3
Accession Version	NM_005312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D21239.1, BC041710.1, BQ054243.1, BQ772947.1 and AJ420417.1. On Jan 14, 2015 this sequence version replaced NM_005312.2. Transcript Variant: This variant (1) uses an alternate 5'-terminal exon which results in a distinct 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (a) has a shorter and distinct N-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D21239.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAGGCAACAG ACAGATTTCT ACCAGAGGGC 
TACCCTCTCC CCTTGGATCT GGAGCAGCAG GCAGTAGAAT TTATGTCCAC CAGTGCTGTG 
GCTTCCAGGT CTCAAAGGCA GAAGAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTCTCTTCCT GCTATAGCCG AGTGTACCAA 
AGCCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC TGTGAAGGGG GTCATCAAGG CTGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCAT CGAGAAGCAG GGACGTCCGT CTCCGACGAG CCCCGTGAAG 
CCCAGTTCCC CTGCCAGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAGATCC TAAACAAGAC GACTGGGATG TCACAGTCAA CTGAGCTCCT CCCAGATGCC 
ACGGATGAAG AGGTCGCGCC CCCCAAGCCT CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CGCCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGATGTTGA CTGTTACGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTCTTGG CCCTCCTTTC CAGCTGCCTC TTGGCGGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GATACGCCAC CTGCTCTCCC CGAGAAGAAG 
CGCAGGAGCG CAGCCTCCCA GACGGCGGAC GGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTATGACAA CATCTCTGGG GAGGACCTGC AGAGCACAGC CCCGATCCCA 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTGCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAGTCAA CCGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGTT GCTGGAGGAC 
TACTCGGAGC CGCAGCCCTC TATGTTCTAC CAGACGCCAC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG TGGGGTGGAC 
TCCGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005303.2
CDS	125..3358
Translation	MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_005312.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005312.3

ORF Insert Sequence:

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAGGCAACAG ACAGATTTCT ACCAGAGGGC 
TACCCTCTCC CCTTGGATCT GGAGCAGCAG GCAGTAGAAT TTATGTCCAC CAGTGCTGTG 
GCTTCCAGGT CTCAAAGGCA GAAGAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTCTCTTCCT GCTATAGCCG AGTGTACCAA 
AGCCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGCGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC TGTGAAGGGG GTCATCAAGG CTGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCAT CGAGAAGCAG GGACGTCCGT CTCCGACGAG CCCCGTGAAG 
CCCAGTTCCC CTGCCAGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAGATCC TAAACAAGAC GACTGGGATG TCACAGTCAA CTGAGCTCCT CCCAGATGCC 
ACGGATGAAG AGGTCGCGCC CCCCAAGCCT CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CGCCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGATGTTGA CTGTTACGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTCTTGG CCCTCCTTTC CAGCTGCCTC TTGGCGGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GATACGCCAC CTGCTCTCCC CGAGAAGAAG 
CGCAGGAGCG CAGCCTCCCA GACGGCGGAC GGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTATGACAA CATCTCTGGG GAGGACCTGC AGAGCACAGC CCCGATCCCA 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTGCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAGTCAA CCGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGTT GCTGGAGGAC 
TACTCGGAGC CGCAGCCCTC TATGTTCTAC CAGACGCCAC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG TGGGGTGGAC 
TCCGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( NM_001304275.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291204.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 3, mRNA.

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CloneID	OHu43223
Clone ID Related Accession (Same CDS sequence)	NM_001304275.1
Accession Version	NM_001304275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF058595.1, CR749354.1, BC041710.1, CN360609.1, BQ772947.1 and AA761516.1. This sequence is a reference standard in the RefSeqGene project. On Jan 14, 2015 this sequence version replaced XM_005272195.1. Transcript Variant: This variant (3) uses an alternate 5'-terminal exon which results in a distinct 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (c) has a shorter and distinct N-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCTTCC TGCTATAGCC GAGTGTACCA AAGCCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CTGTGAAGGG GGTCATCAAG GCTGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCA TCGAGAAGCA GGGACGTCCG TCTCCGACGA GCCCCGTGAA GCCCAGTTCC 
CCTGCCAGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAGATC 
CTAAACAAGA CGACTGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGATGC CACGGATGAA 
GAGGTCGCGC CCCCCAAGCC TCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCGCCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGATGTTG ACTGTTACGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTCTTG GCCCTCCTTT CCAGCTGCCT CTTGGCGGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGATACGCCA CCTGCTCTCC CCGAGAAGAA GCGCAGGAGC 
GCAGCCTCCC AGACGGCGGA CGGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTATGACA ACATCTCTGG GGAGGACCTG CAGAGCACAG CCCCGATCCC ATCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTGCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAGTCA ACCGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGT TGCTGGAGGA CTACTCGGAG 
CCGCAGCCCT CTATGTTCTA CCAGACGCCA CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GTGGGGTGGA CTCCGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291204.1
CDS	115..3399
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	NM_001304275.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304275.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCTTCC TGCTATAGCC GAGTGTACCA AAGCCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CTGTGAAGGG GGTCATCAAG GCTGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCA TCGAGAAGCA GGGACGTCCG TCTCCGACGA GCCCCGTGAA GCCCAGTTCC 
CCTGCCAGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAGATC 
CTAAACAAGA CGACTGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGATGC CACGGATGAA 
GAGGTCGCGC CCCCCAAGCC TCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCGCCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGATGTTG ACTGTTACGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTCTTG GCCCTCCTTT CCAGCTGCCT CTTGGCGGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGATACGCCA CCTGCTCTCC CCGAGAAGAA GCGCAGGAGC 
GCAGCCTCCC AGACGGCGGA CGGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTATGACA ACATCTCTGG GGAGGACCTG CAGAGCACAG CCCCGATCCC ATCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTGCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAGTCA ACCGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGT TGCTGGAGGA CTACTCGGAG 
CCGCAGCCCT CTATGTTCTA CCAGACGCCA CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GTGGGGTGGA CTCCGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGGAGCC ACCGGCTGGG 
AAAGACGGAC ATCCCAGAGA TCCCTCAGCG GTCAGCGGCG TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATTGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGACGTCCGC GGAGGATCTG GGGACATCTT ACTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCATTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAATTC 
TCTCCCTTTG CCGACACATT CAAGAAGCGC GTCAGCAAGA ACACGTTCTT CGTGCTGGTA 
CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAATGGG GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTACTCAGGT GTGCCACCTC CAGCCAGCCC 
CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATAGCGGAGC AGCTAACGCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAGAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTGAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCGGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC CTGGCCGAGT ACTGCACACT GATCGACAGC 
TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GACGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGC 
TGCTTCCAGC AGGCGCACTA TGACATGCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TGTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_006717067.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717130.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

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CloneID	OHu43212
Clone ID Related Accession (Same CDS sequence)	XM_006717067.3
Accession Version	XM_006717067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTCTCCC CTTGGATCTG GAGCAGCAGG CAGTAGAATT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCCT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCAATTGAT 
AAGAAGGTAC TGGAGATGCT TCCAGGGTCA GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA ACGATCCTCG AATTCAGCAC AGCTCAGCCC TCTCTTCCTG CTATAGCCGA 
GTGTACCAAA GCCTCGCCAA CCTCATTCGC TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ACAAGGAGAT GGTGACGACT GTGAAGGGGG TCATCAAGGC TGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG GCTCACCATC GAGAAGCAGG GACGTCCGTC TCCGACGAGC 
CCCGTGAAGC CCAGTTCCCC TGCCAGCAAG CCTGATGGCC CAGCAGAGCT CCCCCTGACA 
GACCGCGAGG TAGAGATCCT AAACAAGACG ACTGGGATGT CACAGTCAAC TGAGCTCCTC 
CCAGATGCCA CGGATGAAGA GGTCGCGCCC CCCAAGCCTC CTCTGCCTGG CATTCGGGTG 
GTTGATAATA GTCCTCCACC AGCATTGCCA CCCAAGAAAA GACAGTCGGC GCCGTCCCCT 
ACCCGAGTGG CTGTGGTGGC CCCCATGAGC CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATCAATA GGCAGGATTT TGATGTTGAC TGTTACGCAC AGAGGCGACT GTCAGGAGGC 
AGCCACTCAT ATGGTGGAGA GTCGCCCCGC CTCTCCCCCT GCAGCAGCAT AGGCAAGCTC 
AGCAAGTCAG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GTGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA CCACTATGAT CCCGACTATG AATTCCTCCA GCAAGACCTC 
TCTAACGCAG ACCAGATACC TCAGCAGACG GCCTGGAACC TTAGCCCGTT GCCAGAGTCT 
TTGGGGGAGT CTGGGTCTCC ATTTCTTGGC CCTCCTTTCC AGCTGCCTCT TGGCGGCCAT 
CCCCAGCCAG ACGGACCTCT GGCCCCAGGG CAGCAGACAG ATACGCCACC TGCTCTCCCC 
GAGAAGAAGC GCAGGAGCGC AGCCTCCCAG ACGGCGGACG GCTCTGGCTG CAGGGTGTCC 
TACGAGCGGC ATCCCTCGCA GTATGACAAC ATCTCTGGGG AGGACCTGCA GAGCACAGCC 
CCGATCCCAT CCGTCCCCTA CGCGCCCTTT GCTGCTATTC TGCCCTTTCA GCATGGAGGT 
TCCTCAGCCC CTGTCGAATT TGTGGGTGAT TTTACTGCTC CTGAGTCAAC CGGTGACCCA 
GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGTTG 
CTGGAGGACT ACTCGGAGCC GCAGCCCTCT ATGTTCTACC AGACGCCACA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTATACG GCTTCAGCGA CTCCTTCAGT 
GGGGTGGACT CCGTGCAGGA GCTGGCCCCG CCGCCCGCCC TACCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCCACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCCTCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTG CCCGTGCCCT CGTATAAGTC TGTGTTTAGG 
TCCTACTCCC AGGATTTCGT GCCTCACCAC CAAGCTTCCG TTCCGCCTTT CCTTCCGCCT 
ACCTCCTCTT CCTCTCCACA TTTCCCACCT GCCCACCAGT CTCAGAGCTC TGACTTAGCC 
GTGCCAACCA TGGCCGGTCC ACCTCCCAGC ACCGTGGACG GGCCTCTCTC GGCTTCTCAG 
GAGAGCAGCT TTCATGGGAA TACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
AGTGAAAACG CCAGTGAGGA AGCTGGTGAG GGTGAATATG TCAATCTGTA TTCCTCTGGC 
CAGAGCAGCG AGGAGCTGGC TCCCTCTCGA GGAGAGCCAC CGGCTGGGAA AGACGGACAT 
CCCAGAGATC CCTCAGCGGT CAGCGGCGTC CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG 
AGGGCCCCAA AGTCACCAGA TGCTCTGGAG TCGGCTCAGT CGGAGGAGGA AGTGGACGAG 
CTGTCCCTCA TTGACCACAA CGAAATTATG TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT 
GACGGGCCGG ACGTCCGCGG AGGATCTGGG GACATCTTAC TGGTCCATGC TACTGAGACT 
GACAGGAAAG ATTTGGTGTT GTACTGCGAG GCATTCCTGA CCACCTACAG GACCTTCATC 
TCCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGATATG AGAAATTCTC TCCCTTTGCC 
GACACATTCA AGAAGCGCGT CAGCAAGAAC ACGTTCTTCG TGCTGGTACG GGTGGTGGAT 
GAGCTCTGCC TGGTGGAGTT GACAGAAGAG ATCCTGAAGC TGCTGATGGA ACTGGTCTTC 
CGCCTGGTGT GCAATGGGGA GCTGAGCCTG GCCCGTGTGC TCCGGAAGAA CATCCTGGAC 
AAGGTGGACC AGAAGAAGCT ACTCAGGTGT GCCACCTCCA GCCAGCCCCT GGCAGCCCGG 
GGGGTAGCAG CCAGGCCGGG GACCTTGCAC GACTTTCACA GCCATGAGAT AGCGGAGCAG 
CTAACGCTGC TGGATGCTGA GCTCTTCTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCAAAAGAGC AGAATGAGGA GAAGAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCCATAATC ATGTTACAGG AAAAGGCCCA GGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTGC GGAAGCTGAA TAACTTCAAC 
TCCTACTTGG CCATCCTCTC TGCCCTGGAC TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG 
AAGCAGACTT CAGAGGGCCT GGCCGAGTAC TGCACACTGA TCGACAGCTC GTCCTCCTTC 
CGAGCCTACC GGGCCGCCCT CTCGGAGGTG GAACCGCCGT GCATCCCGTA CCTGGGGCTG 
ATCCTGCAGG ACCTGACCTT CGTTCACCTG GGAAACCCAG ACTACATCGA CGGGAAAGTG 
AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTCGACA GCATGCGCTG CTTCCAGCAG 
GCGCACTATG ACATGCGGAG GAACGACGAC ATTATAAACT TCTTCAATGA CTTCAGTGAC 
CACCTGGCTG AGGAGGCCCT ATGGGAACTG TCTCTGAAAA TTAAACCCAG GAACATAACA 
AGGAGAAAAA CAGACCGGGA AGAGAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717130.1
CDS	183..3995
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006717067.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717067.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTCTCCC CTTGGATCTG GAGCAGCAGG CAGTAGAATT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCCT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCAATTGAT 
AAGAAGGTAC TGGAGATGCT TCCAGGGTCA GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA ACGATCCTCG AATTCAGCAC AGCTCAGCCC TCTCTTCCTG CTATAGCCGA 
GTGTACCAAA GCCTCGCCAA CCTCATTCGC TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ACAAGGAGAT GGTGACGACT GTGAAGGGGG TCATCAAGGC TGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG GCTCACCATC GAGAAGCAGG GACGTCCGTC TCCGACGAGC 
CCCGTGAAGC CCAGTTCCCC TGCCAGCAAG CCTGATGGCC CAGCAGAGCT CCCCCTGACA 
GACCGCGAGG TAGAGATCCT AAACAAGACG ACTGGGATGT CACAGTCAAC TGAGCTCCTC 
CCAGATGCCA CGGATGAAGA GGTCGCGCCC CCCAAGCCTC CTCTGCCTGG CATTCGGGTG 
GTTGATAATA GTCCTCCACC AGCATTGCCA CCCAAGAAAA GACAGTCGGC GCCGTCCCCT 
ACCCGAGTGG CTGTGGTGGC CCCCATGAGC CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATCAATA GGCAGGATTT TGATGTTGAC TGTTACGCAC AGAGGCGACT GTCAGGAGGC 
AGCCACTCAT ATGGTGGAGA GTCGCCCCGC CTCTCCCCCT GCAGCAGCAT AGGCAAGCTC 
AGCAAGTCAG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GTGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA CCACTATGAT CCCGACTATG AATTCCTCCA GCAAGACCTC 
TCTAACGCAG ACCAGATACC TCAGCAGACG GCCTGGAACC TTAGCCCGTT GCCAGAGTCT 
TTGGGGGAGT CTGGGTCTCC ATTTCTTGGC CCTCCTTTCC AGCTGCCTCT TGGCGGCCAT 
CCCCAGCCAG ACGGACCTCT GGCCCCAGGG CAGCAGACAG ATACGCCACC TGCTCTCCCC 
GAGAAGAAGC GCAGGAGCGC AGCCTCCCAG ACGGCGGACG GCTCTGGCTG CAGGGTGTCC 
TACGAGCGGC ATCCCTCGCA GTATGACAAC ATCTCTGGGG AGGACCTGCA GAGCACAGCC 
CCGATCCCAT CCGTCCCCTA CGCGCCCTTT GCTGCTATTC TGCCCTTTCA GCATGGAGGT 
TCCTCAGCCC CTGTCGAATT TGTGGGTGAT TTTACTGCTC CTGAGTCAAC CGGTGACCCA 
GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGTTG 
CTGGAGGACT ACTCGGAGCC GCAGCCCTCT ATGTTCTACC AGACGCCACA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTATACG GCTTCAGCGA CTCCTTCAGT 
GGGGTGGACT CCGTGCAGGA GCTGGCCCCG CCGCCCGCCC TACCCCCCAA GCAGCGGCAG 
CTGCAGGCCT CCTGTGCTGC TTCTTCCTTC TCCTCTGTCT CCCACTGTGT CCAGCAAACT 
AAAGTGGCCT TCACCCCCGA GGACGGCAGT GCCGCTCAGG GCCTCAGTGT GTCCGTCTCT 
AACTCCTTTC TCAGCCGGCA CGGCAGCTTG CCCGTGCCCT CGTATAAGTC TGTGTTTAGG 
TCCTACTCCC AGGATTTCGT GCCTCACCAC CAAGCTTCCG TTCCGCCTTT CCTTCCGCCT 
ACCTCCTCTT CCTCTCCACA TTTCCCACCT GCCCACCAGT CTCAGAGCTC TGACTTAGCC 
GTGCCAACCA TGGCCGGTCC ACCTCCCAGC ACCGTGGACG GGCCTCTCTC GGCTTCTCAG 
GAGAGCAGCT TTCATGGGAA TACTGTCTGC CTTCCTTCCG AAACCTCTTT CACTGACTCG 
AGTGAAAACG CCAGTGAGGA AGCTGGTGAG GGTGAATATG TCAATCTGTA TTCCTCTGGC 
CAGAGCAGCG AGGAGCTGGC TCCCTCTCGA GGAGAGCCAC CGGCTGGGAA AGACGGACAT 
CCCAGAGATC CCTCAGCGGT CAGCGGCGTC CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG 
AGGGCCCCAA AGTCACCAGA TGCTCTGGAG TCGGCTCAGT CGGAGGAGGA AGTGGACGAG 
CTGTCCCTCA TTGACCACAA CGAAATTATG TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT 
GACGGGCCGG ACGTCCGCGG AGGATCTGGG GACATCTTAC TGGTCCATGC TACTGAGACT 
GACAGGAAAG ATTTGGTGTT GTACTGCGAG GCATTCCTGA CCACCTACAG GACCTTCATC 
TCCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGATATG AGAAATTCTC TCCCTTTGCC 
GACACATTCA AGAAGCGCGT CAGCAAGAAC ACGTTCTTCG TGCTGGTACG GGTGGTGGAT 
GAGCTCTGCC TGGTGGAGTT GACAGAAGAG ATCCTGAAGC TGCTGATGGA ACTGGTCTTC 
CGCCTGGTGT GCAATGGGGA GCTGAGCCTG GCCCGTGTGC TCCGGAAGAA CATCCTGGAC 
AAGGTGGACC AGAAGAAGCT ACTCAGGTGT GCCACCTCCA GCCAGCCCCT GGCAGCCCGG 
GGGGTAGCAG CCAGGCCGGG GACCTTGCAC GACTTTCACA GCCATGAGAT AGCGGAGCAG 
CTAACGCTGC TGGATGCTGA GCTCTTCTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG 
GCAAAAGAGC AGAATGAGGA GAAGAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC 
AACATGTCCT ACTGGGTCCG GTCCATAATC ATGTTACAGG AAAAGGCCCA GGACAGGGAA 
CGGCTGCTCT TGAAGTTCAT CAAGATCATG AAGCACTTGC GGAAGCTGAA TAACTTCAAC 
TCCTACTTGG CCATCCTCTC TGCCCTGGAC TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG 
AAGCAGACTT CAGAGGGCCT GGCCGAGTAC TGCACACTGA TCGACAGCTC GTCCTCCTTC 
CGAGCCTACC GGGCCGCCCT CTCGGAGGTG GAACCGCCGT GCATCCCGTA CCTGGGGCTG 
ATCCTGCAGG ACCTGACCTT CGTTCACCTG GGAAACCCAG ACTACATCGA CGGGAAAGTG 
AACTTCTCCA AGCGGTGGCA GCAGTTCAAC ATCCTCGACA GCATGCGCTG CTTCCAGCAG 
GCGCACTATG ACATGCGGAG GAACGACGAC ATTATAAACT TCTTCAATGA CTTCAGTGAC 
CACCTGGCTG AGGAGGCCCT ATGGGAACTG TCTCTGAAAA TTAAACCCAG GAACATAACA 
AGGAGAAAAA CAGACCGGGA AGAGAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_005272186.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272243.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

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CloneID	OHu43215
Clone ID Related Accession (Same CDS sequence)	XM_005272186.4
Accession Version	XM_005272186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272186.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCTTCC TGCTATAGCC GAGTGTACCA AAGCCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CTGTGAAGGG GGTCATCAAG GCTGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCA TCGAGAAGCA GGGACGTCCG TCTCCGACGA GCCCCGTGAA GCCCAGTTCC 
CCTGCCAGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAGATC 
CTAAACAAGA CGACTGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGATGC CACGGATGAA 
GAGGTCGCGC CCCCCAAGCC TCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCGCCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGATGTTG ACTGTTACGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTCTTG GCCCTCCTTT CCAGCTGCCT CTTGGCGGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGATACGCCA CCTGCTCTCC CCGAGAAGAA GCGCAGGAGC 
GCAGCCTCCC AGACGGCGGA CGGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTATGACA ACATCTCTGG GGAGGACCTG CAGAGCACAG CCCCGATCCC ATCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTGCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAGTCA ACCGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGT TGCTGGAGGA CTACTCGGAG 
CCGCAGCCCT CTATGTTCTA CCAGACGCCA CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GTGGGGTGGA CTCCGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCCACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TGCCCGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAAC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCCTCTC GAGGAGAGCC ACCGGCTGGG AAAGACGGAC ATCCCAGAGA TCCCTCAGCG 
GTCAGCGGCG TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATTGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGACGTCCGC 
GGAGGATCTG GGGACATCTT ACTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCATTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAATTC TCTCCCTTTG CCGACACATT CAAGAAGCGC 
GTCAGCAAGA ACACGTTCTT CGTGCTGGTA CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAATGGG 
GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTACTCAGGT GTGCCACCTC CAGCCAGCCC CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATAGCGGAGC AGCTAACGCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAGAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCGGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC 
CTGGCCGAGT ACTGCACACT GATCGACAGC TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GACGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGC TGCTTCCAGC AGGCGCACTA TGACATGCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TGTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272243.1
CDS	239..4033
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_005272186.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272186.4

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTCTCTTCC TGCTATAGCC GAGTGTACCA AAGCCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGCG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CTGTGAAGGG GGTCATCAAG GCTGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCA TCGAGAAGCA GGGACGTCCG TCTCCGACGA GCCCCGTGAA GCCCAGTTCC 
CCTGCCAGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAGATC 
CTAAACAAGA CGACTGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGATGC CACGGATGAA 
GAGGTCGCGC CCCCCAAGCC TCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCGCCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGATGTTG ACTGTTACGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTCTTG GCCCTCCTTT CCAGCTGCCT CTTGGCGGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGATACGCCA CCTGCTCTCC CCGAGAAGAA GCGCAGGAGC 
GCAGCCTCCC AGACGGCGGA CGGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTATGACA ACATCTCTGG GGAGGACCTG CAGAGCACAG CCCCGATCCC ATCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTGCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAGTCA ACCGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGT TGCTGGAGGA CTACTCGGAG 
CCGCAGCCCT CTATGTTCTA CCAGACGCCA CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GTGGGGTGGA CTCCGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCCACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CACGGCAGCT TGCCCGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAAC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCCTCTC GAGGAGAGCC ACCGGCTGGG AAAGACGGAC ATCCCAGAGA TCCCTCAGCG 
GTCAGCGGCG TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATTGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGACGTCCGC 
GGAGGATCTG GGGACATCTT ACTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCATTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAATTC TCTCCCTTTG CCGACACATT CAAGAAGCGC 
GTCAGCAAGA ACACGTTCTT CGTGCTGGTA CGGGTGGTGG ATGAGCTCTG CCTGGTGGAG 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAATGGG 
GAGCTGAGCC TGGCCCGTGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTACTCAGGT GTGCCACCTC CAGCCAGCCC CTGGCAGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATAGCGGAGC AGCTAACGCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAGAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTGAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCGGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC TTCAGAGGGC 
CTGGCCGAGT ACTGCACACT GATCGACAGC TCGTCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GACGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGC TGCTTCCAGC AGGCGCACTA TGACATGCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TGTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_006717072.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717135.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu43219
Clone ID Related Accession (Same CDS sequence)	XM_006717072.3
Accession Version	XM_006717072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCCA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCCT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTGCCCG TGCCCTCGTA TAAGTCTGTG 
TTTAGGTCCT ACTCCCAGGA TTTCGTGCCT CACCACCAAG CTTCCGTTCC GCCTTTCCTT 
CCGCCTACCT CCTCTTCCTC TCCACATTTC CCACCTGCCC ACCAGTCTCA GAGCTCTGAC 
TTAGCCGTGC CAACCATGGC CGGTCCACCT CCCAGCACCG TGGACGGGCC TCTCTCGGCT 
TCTCAGGAGA GCAGCTTTCA TGGGAATACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717135.1
CDS	193..3891
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006717072.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717072.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCCA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCCT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTGCCCG TGCCCTCGTA TAAGTCTGTG 
TTTAGGTCCT ACTCCCAGGA TTTCGTGCCT CACCACCAAG CTTCCGTTCC GCCTTTCCTT 
CCGCCTACCT CCTCTTCCTC TCCACATTTC CCACCTGCCC ACCAGTCTCA GAGCTCTGAC 
TTAGCCGTGC CAACCATGGC CGGTCCACCT CCCAGCACCG TGGACGGGCC TCTCTCGGCT 
TCTCAGGAGA GCAGCTTTCA TGGGAATACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_005272191.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272248.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

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CloneID	OHu43220
Clone ID Related Accession (Same CDS sequence)	XM_005272191.3
Accession Version	XM_005272191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AAACCTGAGC TGGCTGGAGG AGAAAGAGAA GGAAGTTGTC 
AGTGCCCTGC GCTACTTTAA GACCATTGTG GACAAAATGG CAATTGATAA GAAGGTACTG 
GAGATGCTTC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAAC 
GATCCTCGAA TTCAGCACAG CTCAGCCCTC TCTTCCTGCT ATAGCCGAGT GTACCAAAGC 
CTCGCCAACC TCATTCGCTG GTCTGACCAA GTGATGCTGG AAGGCGTGAA CTCAGAAGAC 
AAGGAGATGG TGACGACTGT GAAGGGGGTC ATCAAGGCTG TGCTGGATGG AGTGAAGGAG 
CTGGTCAGGC TCACCATCGA GAAGCAGGGA CGTCCGTCTC CGACGAGCCC CGTGAAGCCC 
AGTTCCCCTG CCAGCAAGCC TGATGGCCCA GCAGAGCTCC CCCTGACAGA CCGCGAGGTA 
GAGATCCTAA ACAAGACGAC TGGGATGTCA CAGTCAACTG AGCTCCTCCC AGATGCCACG 
GATGAAGAGG TCGCGCCCCC CAAGCCTCCT CTGCCTGGCA TTCGGGTGGT TGATAATAGT 
CCTCCACCAG CATTGCCACC CAAGAAAAGA CAGTCGGCGC CGTCCCCTAC CCGAGTGGCT 
GTGGTGGCCC CCATGAGCCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATCAATAGG 
CAGGATTTTG ATGTTGACTG TTACGCACAG AGGCGACTGT CAGGAGGCAG CCACTCATAT 
GGTGGAGAGT CGCCCCGCCT CTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAC 
GAGCAGCTGT CCTCTCTGGA CAGGGACAGT GGGCAGTGCT CCCGGAACAC AAGCTGTGAA 
ACACTAGACC ACTATGATCC CGACTATGAA TTCCTCCAGC AAGACCTCTC TAACGCAGAC 
CAGATACCTC AGCAGACGGC CTGGAACCTT AGCCCGTTGC CAGAGTCTTT GGGGGAGTCT 
GGGTCTCCAT TTCTTGGCCC TCCTTTCCAG CTGCCTCTTG GCGGCCATCC CCAGCCAGAC 
GGACCTCTGG CCCCAGGGCA GCAGACAGAT ACGCCACCTG CTCTCCCCGA GAAGAAGCGC 
AGGAGCGCAG CCTCCCAGAC GGCGGACGGC TCTGGCTGCA GGGTGTCCTA CGAGCGGCAT 
CCCTCGCAGT ATGACAACAT CTCTGGGGAG GACCTGCAGA GCACAGCCCC GATCCCATCC 
GTCCCCTACG CGCCCTTTGC TGCTATTCTG CCCTTTCAGC ATGGAGGTTC CTCAGCCCCT 
GTCGAATTTG TGGGTGATTT TACTGCTCCT GAGTCAACCG GTGACCCAGA AAAACCACCT 
CCTCTACCAG AGAAGAAAAA CAAACACATG CTGGCCTACA TGCAGTTGCT GGAGGACTAC 
TCGGAGCCGC AGCCCTCTAT GTTCTACCAG ACGCCACAGA ACGAGCACAT CTACCAGCAG 
AAGAACAAGC TCCTCATGGA GGTATACGGC TTCAGCGACT CCTTCAGTGG GGTGGACTCC 
GTGCAGGAGC TGGCCCCGCC GCCCGCCCTA CCCCCCAAGC AGCGGCAGCT GCAGGCCTCC 
TGTGCTGCTT CTTCCTTCTC CTCTGTCTCC CACTGTGTCC AGCAAACTAA AGTGGCCTTC 
ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC CTCAGTGTGT CCGTCTCTAA CTCCTTTCTC 
AGCCGGCACG GCAGCTTGCC CGTGCCCTCG TATAAGTCTG TGTTTAGGTC CTACTCCCAG 
GATTTCGTGC CTCACCACCA AGCTTCCGTT CCGCCTTTCC TTCCGCCTAC CTCCTCTTCC 
TCTCCACATT TCCCACCTGC CCACCAGTCT CAGAGCTCTG ACTTAGCCGT GCCAACCATG 
GCCGGTCCAC CTCCCAGCAC CGTGGACGGG CCTCTCTCGG CTTCTCAGGA GAGCAGCTTT 
CATGGGAATA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGAG TGAAAACGCC 
AGTGAGGAAG CTGGTGAGGG TGAATATGTC AATCTGTATT CCTCTGGCCA GAGCAGCGAG 
GAGCTGGCTC CCTCTCGAGG AGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272248.1
CDS	196..3876
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_005272191.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272191.3

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AAACCTGAGC TGGCTGGAGG AGAAAGAGAA GGAAGTTGTC 
AGTGCCCTGC GCTACTTTAA GACCATTGTG GACAAAATGG CAATTGATAA GAAGGTACTG 
GAGATGCTTC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAAC 
GATCCTCGAA TTCAGCACAG CTCAGCCCTC TCTTCCTGCT ATAGCCGAGT GTACCAAAGC 
CTCGCCAACC TCATTCGCTG GTCTGACCAA GTGATGCTGG AAGGCGTGAA CTCAGAAGAC 
AAGGAGATGG TGACGACTGT GAAGGGGGTC ATCAAGGCTG TGCTGGATGG AGTGAAGGAG 
CTGGTCAGGC TCACCATCGA GAAGCAGGGA CGTCCGTCTC CGACGAGCCC CGTGAAGCCC 
AGTTCCCCTG CCAGCAAGCC TGATGGCCCA GCAGAGCTCC CCCTGACAGA CCGCGAGGTA 
GAGATCCTAA ACAAGACGAC TGGGATGTCA CAGTCAACTG AGCTCCTCCC AGATGCCACG 
GATGAAGAGG TCGCGCCCCC CAAGCCTCCT CTGCCTGGCA TTCGGGTGGT TGATAATAGT 
CCTCCACCAG CATTGCCACC CAAGAAAAGA CAGTCGGCGC CGTCCCCTAC CCGAGTGGCT 
GTGGTGGCCC CCATGAGCCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATCAATAGG 
CAGGATTTTG ATGTTGACTG TTACGCACAG AGGCGACTGT CAGGAGGCAG CCACTCATAT 
GGTGGAGAGT CGCCCCGCCT CTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAC 
GAGCAGCTGT CCTCTCTGGA CAGGGACAGT GGGCAGTGCT CCCGGAACAC AAGCTGTGAA 
ACACTAGACC ACTATGATCC CGACTATGAA TTCCTCCAGC AAGACCTCTC TAACGCAGAC 
CAGATACCTC AGCAGACGGC CTGGAACCTT AGCCCGTTGC CAGAGTCTTT GGGGGAGTCT 
GGGTCTCCAT TTCTTGGCCC TCCTTTCCAG CTGCCTCTTG GCGGCCATCC CCAGCCAGAC 
GGACCTCTGG CCCCAGGGCA GCAGACAGAT ACGCCACCTG CTCTCCCCGA GAAGAAGCGC 
AGGAGCGCAG CCTCCCAGAC GGCGGACGGC TCTGGCTGCA GGGTGTCCTA CGAGCGGCAT 
CCCTCGCAGT ATGACAACAT CTCTGGGGAG GACCTGCAGA GCACAGCCCC GATCCCATCC 
GTCCCCTACG CGCCCTTTGC TGCTATTCTG CCCTTTCAGC ATGGAGGTTC CTCAGCCCCT 
GTCGAATTTG TGGGTGATTT TACTGCTCCT GAGTCAACCG GTGACCCAGA AAAACCACCT 
CCTCTACCAG AGAAGAAAAA CAAACACATG CTGGCCTACA TGCAGTTGCT GGAGGACTAC 
TCGGAGCCGC AGCCCTCTAT GTTCTACCAG ACGCCACAGA ACGAGCACAT CTACCAGCAG 
AAGAACAAGC TCCTCATGGA GGTATACGGC TTCAGCGACT CCTTCAGTGG GGTGGACTCC 
GTGCAGGAGC TGGCCCCGCC GCCCGCCCTA CCCCCCAAGC AGCGGCAGCT GCAGGCCTCC 
TGTGCTGCTT CTTCCTTCTC CTCTGTCTCC CACTGTGTCC AGCAAACTAA AGTGGCCTTC 
ACCCCCGAGG ACGGCAGTGC CGCTCAGGGC CTCAGTGTGT CCGTCTCTAA CTCCTTTCTC 
AGCCGGCACG GCAGCTTGCC CGTGCCCTCG TATAAGTCTG TGTTTAGGTC CTACTCCCAG 
GATTTCGTGC CTCACCACCA AGCTTCCGTT CCGCCTTTCC TTCCGCCTAC CTCCTCTTCC 
TCTCCACATT TCCCACCTGC CCACCAGTCT CAGAGCTCTG ACTTAGCCGT GCCAACCATG 
GCCGGTCCAC CTCCCAGCAC CGTGGACGGG CCTCTCTCGG CTTCTCAGGA GAGCAGCTTT 
CATGGGAATA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGAG TGAAAACGCC 
AGTGAGGAAG CTGGTGAGGG TGAATATGTC AATCTGTATT CCTCTGGCCA GAGCAGCGAG 
GAGCTGGCTC CCTCTCGAGG AGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_006717074.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717137.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.

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CloneID	OHu43222
Clone ID Related Accession (Same CDS sequence)	XM_006717074.3
Accession Version	XM_006717074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTCTCCC CTTGGATCTG GAGCAGCAGG CAGTAGAATT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCCT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCAATTGAT 
AAGAAGGTAC TGGAGATGCT TCCAGGGTCA GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA ACGATCCTCG AATTCAGCAC AGCTCAGCCC TCTCTTCCTG CTATAGCCGA 
GTGTACCAAA GCCTCGCCAA CCTCATTCGC TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ACAAGGAGAT GGTGACGACT GTGAAGGGGG TCATCAAGGC TGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG GCTCACCATC GAGAAGCAGG GACGTCCGTC TCCGACGAGC 
CCCGTGAAGC CCAGTTCCCC TGCCAGCAAG CCTGATGGCC CAGCAGAGCT CCCCCTGACA 
GACCGCGAGG TAGAGATCCT AAACAAGACG ACTGGGATGT CACAGTCAAC TGAGCTCCTC 
CCAGATGCCA CGGATGAAGA GGTCGCGCCC CCCAAGCCTC CTCTGCCTGG CATTCGGGTG 
GTTGATAATA GTCCTCCACC AGCATTGCCA CCCAAGAAAA GACAGTCGGC GCCGTCCCCT 
ACCCGAGTGG CTGTGGTGGC CCCCATGAGC CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATCAATA GGCAGGATTT TGATGTTGAC TGTTACGCAC AGAGGCGACT GTCAGGAGGC 
AGCCACTCAT ATGGTGGAGA GTCGCCCCGC CTCTCCCCCT GCAGCAGCAT AGGCAAGCTC 
AGCAAGTCAG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GTGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA CCACTATGAT CCCGACTATG AATTCCTCCA GCAAGACCTC 
TCTAACGCAG ACCAGATACC TCAGCAGACG GCCTGGAACC TTAGCCCGTT GCCAGAGTCT 
TTGGGGGAGT CTGGGTCTCC ATTTCTTGGC CCTCCTTTCC AGCTGCCTCT TGGCGGCCAT 
CCCCAGCCAG ACGGACCTCT GGCCCCAGGG CAGCAGACAG ATACGCCACC TGCTCTCCCC 
GAGAAGAAGC GCAGGAGCGC AGCCTCCCAG ACGGCGGACG GCTCTGGCTG CAGGGTGTCC 
TACGAGCGGC ATCCCTCGCA GTATGACAAC ATCTCTGGGG AGGACCTGCA GAGCACAGCC 
CCGATCCCAT CCGTCCCCTA CGCGCCCTTT GCTGCTATTC TGCCCTTTCA GCATGGAGGT 
TCCTCAGCCC CTGTCGAATT TGTGGGTGAT TTTACTGCTC CTGAGTCAAC CGGTGACCCA 
GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGTTG 
CTGGAGGACT ACTCGGAGCC GCAGCCCTCT ATGTTCTACC AGACGCCACA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTATACG GCTTCAGCGA CTCCTTCAGT 
GGGGTGGACT CCGTGCAGGA GCTGGCCCCG CCGCCCGCCC TACCCCCCAA GCAGCGGCAG 
CTGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717137.1
CDS	183..3485
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_006717074.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717074.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AGGCAACAGA CAGATTTCTA 
CCAGAGGGCT ACCCTCTCCC CTTGGATCTG GAGCAGCAGG CAGTAGAATT TATGTCCACC 
AGTGCTGTGG CTTCCAGGTC TCAAAGGCAG AAGAACCTGA GCTGGCTGGA GGAGAAAGAG 
AAGGAAGTTG TCAGTGCCCT GCGCTACTTT AAGACCATTG TGGACAAAAT GGCAATTGAT 
AAGAAGGTAC TGGAGATGCT TCCAGGGTCA GCCAGCAAGG TGCTGGAGGC CATCTTACCC 
CTGGTGCAGA ACGATCCTCG AATTCAGCAC AGCTCAGCCC TCTCTTCCTG CTATAGCCGA 
GTGTACCAAA GCCTCGCCAA CCTCATTCGC TGGTCTGACC AAGTGATGCT GGAAGGCGTG 
AACTCAGAAG ACAAGGAGAT GGTGACGACT GTGAAGGGGG TCATCAAGGC TGTGCTGGAT 
GGAGTGAAGG AGCTGGTCAG GCTCACCATC GAGAAGCAGG GACGTCCGTC TCCGACGAGC 
CCCGTGAAGC CCAGTTCCCC TGCCAGCAAG CCTGATGGCC CAGCAGAGCT CCCCCTGACA 
GACCGCGAGG TAGAGATCCT AAACAAGACG ACTGGGATGT CACAGTCAAC TGAGCTCCTC 
CCAGATGCCA CGGATGAAGA GGTCGCGCCC CCCAAGCCTC CTCTGCCTGG CATTCGGGTG 
GTTGATAATA GTCCTCCACC AGCATTGCCA CCCAAGAAAA GACAGTCGGC GCCGTCCCCT 
ACCCGAGTGG CTGTGGTGGC CCCCATGAGC CGAGCCACCA GTGGCTCCAG TTTGCCTGTT 
GGAATCAATA GGCAGGATTT TGATGTTGAC TGTTACGCAC AGAGGCGACT GTCAGGAGGC 
AGCCACTCAT ATGGTGGAGA GTCGCCCCGC CTCTCCCCCT GCAGCAGCAT AGGCAAGCTC 
AGCAAGTCAG ACGAGCAGCT GTCCTCTCTG GACAGGGACA GTGGGCAGTG CTCCCGGAAC 
ACAAGCTGTG AAACACTAGA CCACTATGAT CCCGACTATG AATTCCTCCA GCAAGACCTC 
TCTAACGCAG ACCAGATACC TCAGCAGACG GCCTGGAACC TTAGCCCGTT GCCAGAGTCT 
TTGGGGGAGT CTGGGTCTCC ATTTCTTGGC CCTCCTTTCC AGCTGCCTCT TGGCGGCCAT 
CCCCAGCCAG ACGGACCTCT GGCCCCAGGG CAGCAGACAG ATACGCCACC TGCTCTCCCC 
GAGAAGAAGC GCAGGAGCGC AGCCTCCCAG ACGGCGGACG GCTCTGGCTG CAGGGTGTCC 
TACGAGCGGC ATCCCTCGCA GTATGACAAC ATCTCTGGGG AGGACCTGCA GAGCACAGCC 
CCGATCCCAT CCGTCCCCTA CGCGCCCTTT GCTGCTATTC TGCCCTTTCA GCATGGAGGT 
TCCTCAGCCC CTGTCGAATT TGTGGGTGAT TTTACTGCTC CTGAGTCAAC CGGTGACCCA 
GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGTTG 
CTGGAGGACT ACTCGGAGCC GCAGCCCTCT ATGTTCTACC AGACGCCACA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTATACG GCTTCAGCGA CTCCTTCAGT 
GGGGTGGACT CCGTGCAGGA GCTGGCCCCG CCGCCCGCCC TACCCCCCAA GCAGCGGCAG 
CTGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518569.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516871.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58056
Clone ID Related Accession (Same CDS sequence)	XM_011518569.2 , XM_011518569.3
Accession Version	XM_011518569.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGATTTGG TGTTGTACTG CGAGGCATTC 
CTGACCACCT ACAGGACCTT CATCTCCCCA GAGGAGCTCA TCAAGAAGCT GCAGTACAGA 
TATGAGAAAT TCTCTCCCTT TGCCGACACA TTCAAGAAGC GCGTCAGCAA GAACACGTTC 
TTCGTGCTGG TACGGGTGGT GGATGAGCTC TGCCTGGTGG AGTTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAACTGGT CTTCCGCCTG GTGTGCAATG GGGAGCTGAG CCTGGCCCGT 
GTGCTCCGGA AGAACATCCT GGACAAGGTG GACCAGAAGA AGCTACTCAG GTGTGCCACC 
TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA GCAGCCAGGC CGGGGACCTT GCACGACTTT 
CACAGCCATG AGATAGCGGA GCAGCTAACG CTGCTGGATG CTGAGCTCTT CTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCAAAA GAGCAGAATG AGGAGAAGAG CCCCAACTTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCCAT AATCATGTTA 
CAGGAAAAGG CCCAGGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTGCGGAAGC TGAATAACTT CAACTCCTAC TTGGCCATCC TCTCTGCCCT GGACTCGGCG 
CCCATCCGCA GGCTGGAGTG GCAGAAGCAG ACTTCAGAGG GCCTGGCCGA GTACTGCACA 
CTGATCGACA GCTCGTCCTC CTTCCGAGCC TACCGGGCCG CCCTCTCGGA GGTGGAACCG 
CCGTGCATCC CGTACCTGGG GCTGATCCTG CAGGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGACTACA TCGACGGGAA AGTGAACTTC TCCAAGCGGT GGCAGCAGTT CAACATCCTC 
GACAGCATGC GCTGCTTCCA GCAGGCGCAC TATGACATGC GGAGGAACGA CGACATTATA 
AACTTCTTCA ATGACTTCAG TGACCACCTG GCTGAGGAGG CCCTATGGGA ACTGTCTCTG 
AAAATTAAAC CCAGGAACAT AACAAGGAGA AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516871.1
CDS	284..4120
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518569.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518569.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGATTTGG TGTTGTACTG CGAGGCATTC 
CTGACCACCT ACAGGACCTT CATCTCCCCA GAGGAGCTCA TCAAGAAGCT GCAGTACAGA 
TATGAGAAAT TCTCTCCCTT TGCCGACACA TTCAAGAAGC GCGTCAGCAA GAACACGTTC 
TTCGTGCTGG TACGGGTGGT GGATGAGCTC TGCCTGGTGG AGTTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAACTGGT CTTCCGCCTG GTGTGCAATG GGGAGCTGAG CCTGGCCCGT 
GTGCTCCGGA AGAACATCCT GGACAAGGTG GACCAGAAGA AGCTACTCAG GTGTGCCACC 
TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA GCAGCCAGGC CGGGGACCTT GCACGACTTT 
CACAGCCATG AGATAGCGGA GCAGCTAACG CTGCTGGATG CTGAGCTCTT CTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCAAAA GAGCAGAATG AGGAGAAGAG CCCCAACTTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCCAT AATCATGTTA 
CAGGAAAAGG CCCAGGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTGCGGAAGC TGAATAACTT CAACTCCTAC TTGGCCATCC TCTCTGCCCT GGACTCGGCG 
CCCATCCGCA GGCTGGAGTG GCAGAAGCAG ACTTCAGAGG GCCTGGCCGA GTACTGCACA 
CTGATCGACA GCTCGTCCTC CTTCCGAGCC TACCGGGCCG CCCTCTCGGA GGTGGAACCG 
CCGTGCATCC CGTACCTGGG GCTGATCCTG CAGGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGACTACA TCGACGGGAA AGTGAACTTC TCCAAGCGGT GGCAGCAGTT CAACATCCTC 
GACAGCATGC GCTGCTTCCA GCAGGCGCAC TATGACATGC GGAGGAACGA CGACATTATA 
AACTTCTTCA ATGACTTCAG TGACCACCTG GCTGAGGAGG CCCTATGGGA ACTGTCTCTG 
AAAATTAAAC CCAGGAACAT AACAAGGAGA AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518569.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516871.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

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CloneID	OHu58056
Clone ID Related Accession (Same CDS sequence)	XM_011518569.2 , XM_011518569.3
Accession Version	XM_011518569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGATTTGG TGTTGTACTG CGAGGCATTC 
CTGACCACCT ACAGGACCTT CATCTCCCCA GAGGAGCTCA TCAAGAAGCT GCAGTACAGA 
TATGAGAAAT TCTCTCCCTT TGCCGACACA TTCAAGAAGC GCGTCAGCAA GAACACGTTC 
TTCGTGCTGG TACGGGTGGT GGATGAGCTC TGCCTGGTGG AGTTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAACTGGT CTTCCGCCTG GTGTGCAATG GGGAGCTGAG CCTGGCCCGT 
GTGCTCCGGA AGAACATCCT GGACAAGGTG GACCAGAAGA AGCTACTCAG GTGTGCCACC 
TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA GCAGCCAGGC CGGGGACCTT GCACGACTTT 
CACAGCCATG AGATAGCGGA GCAGCTAACG CTGCTGGATG CTGAGCTCTT CTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCAAAA GAGCAGAATG AGGAGAAGAG CCCCAACTTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCCAT AATCATGTTA 
CAGGAAAAGG CCCAGGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTGCGGAAGC TGAATAACTT CAACTCCTAC TTGGCCATCC TCTCTGCCCT GGACTCGGCG 
CCCATCCGCA GGCTGGAGTG GCAGAAGCAG ACTTCAGAGG GCCTGGCCGA GTACTGCACA 
CTGATCGACA GCTCGTCCTC CTTCCGAGCC TACCGGGCCG CCCTCTCGGA GGTGGAACCG 
CCGTGCATCC CGTACCTGGG GCTGATCCTG CAGGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGACTACA TCGACGGGAA AGTGAACTTC TCCAAGCGGT GGCAGCAGTT CAACATCCTC 
GACAGCATGC GCTGCTTCCA GCAGGCGCAC TATGACATGC GGAGGAACGA CGACATTATA 
AACTTCTTCA ATGACTTCAG TGACCACCTG GCTGAGGAGG CCCTATGGGA ACTGTCTCTG 
AAAATTAAAC CCAGGAACAT AACAAGGAGA AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516871.1
CDS	285..4121
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518569.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518569.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGAGTGAA AACGCCAGTG AGGAAGCTGG TGAGGGTGAA 
TATGTCAATC TGTATTCCTC TGGCCAGAGC AGCGAGGAGC TGGCTCCCTC TCGAGGAGAG 
CCACCGGCTG GGAAAGACGG ACATCCCAGA GATCCCTCAG CGGTCAGCGG CGTCCCTGGG 
AAGGACAGCA GAGACGGCAG TGAGAGGGCC CCAAAGTCAC CAGATGCTCT GGAGTCGGCT 
CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC CTCATTGACC ACAACGAAAT TATGTCCAGG 
CTGACGCTCA AGCAGGAGGG TGATGACGGG CCGGACGTCC GCGGAGGATC TGGGGACATC 
TTACTGGTCC ATGCTACTGA GACTGACAGG AAAGATTTGG TGTTGTACTG CGAGGCATTC 
CTGACCACCT ACAGGACCTT CATCTCCCCA GAGGAGCTCA TCAAGAAGCT GCAGTACAGA 
TATGAGAAAT TCTCTCCCTT TGCCGACACA TTCAAGAAGC GCGTCAGCAA GAACACGTTC 
TTCGTGCTGG TACGGGTGGT GGATGAGCTC TGCCTGGTGG AGTTGACAGA AGAGATCCTG 
AAGCTGCTGA TGGAACTGGT CTTCCGCCTG GTGTGCAATG GGGAGCTGAG CCTGGCCCGT 
GTGCTCCGGA AGAACATCCT GGACAAGGTG GACCAGAAGA AGCTACTCAG GTGTGCCACC 
TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA GCAGCCAGGC CGGGGACCTT GCACGACTTT 
CACAGCCATG AGATAGCGGA GCAGCTAACG CTGCTGGATG CTGAGCTCTT CTATAAAATA 
GAGATTCCTG AGGTTTTGCT TTGGGCAAAA GAGCAGAATG AGGAGAAGAG CCCCAACTTG 
ACCCAGTTCA CGGAGCACTT CAACAACATG TCCTACTGGG TCCGGTCCAT AATCATGTTA 
CAGGAAAAGG CCCAGGACAG GGAACGGCTG CTCTTGAAGT TCATCAAGAT CATGAAGCAC 
TTGCGGAAGC TGAATAACTT CAACTCCTAC TTGGCCATCC TCTCTGCCCT GGACTCGGCG 
CCCATCCGCA GGCTGGAGTG GCAGAAGCAG ACTTCAGAGG GCCTGGCCGA GTACTGCACA 
CTGATCGACA GCTCGTCCTC CTTCCGAGCC TACCGGGCCG CCCTCTCGGA GGTGGAACCG 
CCGTGCATCC CGTACCTGGG GCTGATCCTG CAGGACCTGA CCTTCGTTCA CCTGGGAAAC 
CCAGACTACA TCGACGGGAA AGTGAACTTC TCCAAGCGGT GGCAGCAGTT CAACATCCTC 
GACAGCATGC GCTGCTTCCA GCAGGCGCAC TATGACATGC GGAGGAACGA CGACATTATA 
AACTTCTTCA ATGACTTCAG TGACCACCTG GCTGAGGAGG CCCTATGGGA ACTGTCTCTG 
AAAATTAAAC CCAGGAACAT AACAAGGAGA AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516872.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu58057
Clone ID Related Accession (Same CDS sequence)	XM_011518570.2 , XM_011518570.3
Accession Version	XM_011518570.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAC GCCAGTGAGG AAGCTGGTGA GGGTGAATAT 
GTCAATCTGT ATTCCTCTGG CCAGAGCAGC GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516872.1
CDS	176..4009
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518570.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAC GCCAGTGAGG AAGCTGGTGA GGGTGAATAT 
GTCAATCTGT ATTCCTCTGG CCAGAGCAGC GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518570.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516872.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu58057
Clone ID Related Accession (Same CDS sequence)	XM_011518570.2 , XM_011518570.3
Accession Version	XM_011518570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAC GCCAGTGAGG AAGCTGGTGA GGGTGAATAT 
GTCAATCTGT ATTCCTCTGG CCAGAGCAGC GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516872.1
CDS	177..4010
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518570.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518570.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GAGTGAAAAC GCCAGTGAGG AAGCTGGTGA GGGTGAATAT 
GTCAATCTGT ATTCCTCTGG CCAGAGCAGC GAGGAGCTGG CTCCCTCTCG AGGAGAGCCA 
CCGGCTGGGA AAGACGGACA TCCCAGAGAT CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG 
GACAGCAGAG ACGGCAGTGA GAGGGCCCCA AAGTCACCAG ATGCTCTGGA GTCGGCTCAG 
TCGGAGGAGG AAGTGGACGA GCTGTCCCTC ATTGACCACA ACGAAATTAT GTCCAGGCTG 
ACGCTCAAGC AGGAGGGTGA TGACGGGCCG GACGTCCGCG GAGGATCTGG GGACATCTTA 
CTGGTCCATG CTACTGAGAC TGACAGGAAA GATTTGGTGT TGTACTGCGA GGCATTCCTG 
ACCACCTACA GGACCTTCAT CTCCCCAGAG GAGCTCATCA AGAAGCTGCA GTACAGATAT 
GAGAAATTCT CTCCCTTTGC CGACACATTC AAGAAGCGCG TCAGCAAGAA CACGTTCTTC 
GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC CTGGTGGAGT TGACAGAAGA GATCCTGAAG 
CTGCTGATGG AACTGGTCTT CCGCCTGGTG TGCAATGGGG AGCTGAGCCT GGCCCGTGTG 
CTCCGGAAGA ACATCCTGGA CAAGGTGGAC CAGAAGAAGC TACTCAGGTG TGCCACCTCC 
AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA GCCAGGCCGG GGACCTTGCA CGACTTTCAC 
AGCCATGAGA TAGCGGAGCA GCTAACGCTG CTGGATGCTG AGCTCTTCTA TAAAATAGAG 
ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG CAGAATGAGG AGAAGAGCCC CAACTTGACC 
CAGTTCACGG AGCACTTCAA CAACATGTCC TACTGGGTCC GGTCCATAAT CATGTTACAG 
GAAAAGGCCC AGGACAGGGA ACGGCTGCTC TTGAAGTTCA TCAAGATCAT GAAGCACTTG 
CGGAAGCTGA ATAACTTCAA CTCCTACTTG GCCATCCTCT CTGCCCTGGA CTCGGCGCCC 
ATCCGCAGGC TGGAGTGGCA GAAGCAGACT TCAGAGGGCC TGGCCGAGTA CTGCACACTG 
ATCGACAGCT CGTCCTCCTT CCGAGCCTAC CGGGCCGCCC TCTCGGAGGT GGAACCGCCG 
TGCATCCCGT ACCTGGGGCT GATCCTGCAG GACCTGACCT TCGTTCACCT GGGAAACCCA 
GACTACATCG ACGGGAAAGT GAACTTCTCC AAGCGGTGGC AGCAGTTCAA CATCCTCGAC 
AGCATGCGCT GCTTCCAGCA GGCGCACTAT GACATGCGGA GGAACGACGA CATTATAAAC 
TTCTTCAATG ACTTCAGTGA CCACCTGGCT GAGGAGGCCC TATGGGAACT GTCTCTGAAA 
ATTAAACCCA GGAACATAAC AAGGAGAAAA ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518571.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516873.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OHu58058
Clone ID Related Accession (Same CDS sequence)	XM_011518571.2
Accession Version	XM_011518571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAGCCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAATCC AGGTTTCCTC TCTTCCAGGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516873.1
CDS	859..4680
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518571.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518571.2

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAGCCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAATCC AGGTTTCCTC TCTTCCAGGA GGCAACAGAC 
AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT 
ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAAAGGCAGA AGAACCTGAG CTGGCTGGAG 
GAGAAAGAGA AGGAAGTTGT CAGTGCCCTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGATA AGAAGGTACT GGAGATGCTT CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC 
ATCTTACCCC TGGTGCAGAA CGATCCTCGA ATTCAGCACA GCTCAGCCCT CTCTTCCTGC 
TATAGCCGAG TGTACCAAAG CCTCGCCAAC CTCATTCGCT GGTCTGACCA AGTGATGCTG 
GAAGGCGTGA ACTCAGAAGA CAAGGAGATG GTGACGACTG TGAAGGGGGT CATCAAGGCT 
GTGCTGGATG GAGTGAAGGA GCTGGTCAGG CTCACCATCG AGAAGCAGGG ACGTCCGTCT 
CCGACGAGCC CCGTGAAGCC CAGTTCCCCT GCCAGCAAGC CTGATGGCCC AGCAGAGCTC 
CCCCTGACAG ACCGCGAGGT AGAGATCCTA AACAAGACGA CTGGGATGTC ACAGTCAACT 
GAGCTCCTCC CAGATGCCAC GGATGAAGAG GTCGCGCCCC CCAAGCCTCC TCTGCCTGGC 
ATTCGGGTGG TTGATAATAG TCCTCCACCA GCATTGCCAC CCAAGAAAAG ACAGTCGGCG 
CCGTCCCCTA CCCGAGTGGC TGTGGTGGCC CCCATGAGCC GAGCCACCAG TGGCTCCAGT 
TTGCCTGTTG GAATCAATAG GCAGGATTTT GATGTTGACT GTTACGCACA GAGGCGACTG 
TCAGGAGGCA GCCACTCATA TGGTGGAGAG TCGCCCCGCC TCTCCCCCTG CAGCAGCATA 
GGCAAGCTCA GCAAGTCAGA CGAGCAGCTG TCCTCTCTGG ACAGGGACAG TGGGCAGTGC 
TCCCGGAACA CAAGCTGTGA AACACTAGAC CACTATGATC CCGACTATGA ATTCCTCCAG 
CAAGACCTCT CTAACGCAGA CCAGATACCT CAGCAGACGG CCTGGAACCT TAGCCCGTTG 
CCAGAGTCTT TGGGGGAGTC TGGGTCTCCA TTTCTTGGCC CTCCTTTCCA GCTGCCTCTT 
GGCGGCCATC CCCAGCCAGA CGGACCTCTG GCCCCAGGGC AGCAGACAGA TACGCCACCT 
GCTCTCCCCG AGAAGAAGCG CAGGAGCGCA GCCTCCCAGA CGGCGGACGG CTCTGGCTGC 
AGGGTGTCCT ACGAGCGGCA TCCCTCGCAG TATGACAACA TCTCTGGGGA GGACCTGCAG 
AGCACAGCCC CGATCCCATC CGTCCCCTAC GCGCCCTTTG CTGCTATTCT GCCCTTTCAG 
CATGGAGGTT CCTCAGCCCC TGTCGAATTT GTGGGTGATT TTACTGCTCC TGAGTCAACC 
GGTGACCCAG AAAAACCACC TCCTCTACCA GAGAAGAAAA ACAAACACAT GCTGGCCTAC 
ATGCAGTTGC TGGAGGACTA CTCGGAGCCG CAGCCCTCTA TGTTCTACCA GACGCCACAG 
AACGAGCACA TCTACCAGCA GAAGAACAAG CTCCTCATGG AGGTATACGG CTTCAGCGAC 
TCCTTCAGTG GGGTGGACTC CGTGCAGGAG CTGGCCCCGC CGCCCGCCCT ACCCCCCAAG 
CAGCGGCAGC TGCAGGCCTC CTGTGCTGCT TCTTCCTTCT CCTCTGTCTC CCACTGTGTC 
CAGCAAACTA AAGTGGCCTT CACCCCCGAG GACGGCAGTG CCGCTCAGGG CCTCAGTGTG 
TCCGTCTCTA ACTCCTTTCT CAGCCGGCAC GGCAGCTTGC CCGTGCCCTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACCACC AAGCTTCCGT TCCGCCTTTC 
CTTCCGCCTA CCTCCTCTTC CTCTCCACAT TTCCCACCTG CCCACCAGTC TCAGAGCTCT 
GACTTAGCCG TGCCAACCAT GGCCGGTCCA CCTCCCAGCA CCGTGGACGG GCCTCTCTCG 
GCTTCTCAGG AGAGCAGCTT TCATGGGAAT ACTGTCTGCC TTCCTTCCGA AACCTCTTTC 
ACTGACTCGA GTGAAAACGC CAGTGAGGAA GCTGGTGAGG GTGAATATGT CAATCTGTAT 
TCCTCTGGCC AGAGCAGCGA GGAGCTGGCT CCCTCTCGAG GAGAGCCACC GGCTGGGAAA 
GACGGACATC CCAGAGATCC CTCAGCGGTC AGCGGCGTCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT TGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA CGTCCGCGGA GGATCTGGGG ACATCTTACT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CATTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAATTCTCT 
CCCTTTGCCG ACACATTCAA GAAGCGCGTC AGCAAGAACA CGTTCTTCGT GCTGGTACGG 
GTGGTGGATG AGCTCTGCCT GGTGGAGTTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAATGGGGAG CTGAGCCTGG CCCGTGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTA CTCAGGTGTG CCACCTCCAG CCAGCCCCTG 
GCAGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATA 
GCGGAGCAGC TAACGCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAG AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT GAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CGGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACTTC AGAGGGCCTG GCCGAGTACT GCACACTGAT CGACAGCTCG 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAC 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGCTGC 
TTCCAGCAGG CGCACTATGA CATGCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTGT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518572.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516874.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu58059
Clone ID Related Accession (Same CDS sequence)	XM_011518572.2
Accession Version	XM_011518572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAATCCAGG TTTCCTCTCT TCCAGGAGGC AACAGACAGA 
TTTCTACCAG AGGGCTACCC TCTCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG 
TCCACCAGTG CTGTGGCTTC CAGGTCTCAA AGGCAGAAGA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCCA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCCT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTGCCCG TGCCCTCGTA TAAGTCTGTG 
TTTAGGTCCT ACTCCCAGGA TTTCGTGCCT CACCACCAAG CTTCCGTTCC GCCTTTCCTT 
CCGCCTACCT CCTCTTCCTC TCCACATTTC CCACCTGCCC ACCAGTCTCA GAGCTCTGAC 
TTAGCCGTGC CAACCATGGC CGGTCCACCT CCCAGCACCG TGGACGGGCC TCTCTCGGCT 
TCTCAGGAGA GCAGCTTTCA TGGGAATACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516874.1
CDS	41..3859
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518572.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518572.2

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAATCCAGG TTTCCTCTCT TCCAGGAGGC AACAGACAGA 
TTTCTACCAG AGGGCTACCC TCTCCCCTTG GATCTGGAGC AGCAGGCAGT AGAATTTATG 
TCCACCAGTG CTGTGGCTTC CAGGTCTCAA AGGCAGAAGA ACCTGAGCTG GCTGGAGGAG 
AAAGAGAAGG AAGTTGTCAG TGCCCTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGATAAGA AGGTACTGGA GATGCTTCCA GGGTCAGCCA GCAAGGTGCT GGAGGCCATC 
TTACCCCTGG TGCAGAACGA TCCTCGAATT CAGCACAGCT CAGCCCTCTC TTCCTGCTAT 
AGCCGAGTGT ACCAAAGCCT CGCCAACCTC ATTCGCTGGT CTGACCAAGT GATGCTGGAA 
GGCGTGAACT CAGAAGACAA GGAGATGGTG ACGACTGTGA AGGGGGTCAT CAAGGCTGTG 
CTGGATGGAG TGAAGGAGCT GGTCAGGCTC ACCATCGAGA AGCAGGGACG TCCGTCTCCG 
ACGAGCCCCG TGAAGCCCAG TTCCCCTGCC AGCAAGCCTG ATGGCCCAGC AGAGCTCCCC 
CTGACAGACC GCGAGGTAGA GATCCTAAAC AAGACGACTG GGATGTCACA GTCAACTGAG 
CTCCTCCCAG ATGCCACGGA TGAAGAGGTC GCGCCCCCCA AGCCTCCTCT GCCTGGCATT 
CGGGTGGTTG ATAATAGTCC TCCACCAGCA TTGCCACCCA AGAAAAGACA GTCGGCGCCG 
TCCCCTACCC GAGTGGCTGT GGTGGCCCCC ATGAGCCGAG CCACCAGTGG CTCCAGTTTG 
CCTGTTGGAA TCAATAGGCA GGATTTTGAT GTTGACTGTT ACGCACAGAG GCGACTGTCA 
GGAGGCAGCC ACTCATATGG TGGAGAGTCG CCCCGCCTCT CCCCCTGCAG CAGCATAGGC 
AAGCTCAGCA AGTCAGACGA GCAGCTGTCC TCTCTGGACA GGGACAGTGG GCAGTGCTCC 
CGGAACACAA GCTGTGAAAC ACTAGACCAC TATGATCCCG ACTATGAATT CCTCCAGCAA 
GACCTCTCTA ACGCAGACCA GATACCTCAG CAGACGGCCT GGAACCTTAG CCCGTTGCCA 
GAGTCTTTGG GGGAGTCTGG GTCTCCATTT CTTGGCCCTC CTTTCCAGCT GCCTCTTGGC 
GGCCATCCCC AGCCAGACGG ACCTCTGGCC CCAGGGCAGC AGACAGATAC GCCACCTGCT 
CTCCCCGAGA AGAAGCGCAG GAGCGCAGCC TCCCAGACGG CGGACGGCTC TGGCTGCAGG 
GTGTCCTACG AGCGGCATCC CTCGCAGTAT GACAACATCT CTGGGGAGGA CCTGCAGAGC 
ACAGCCCCGA TCCCATCCGT CCCCTACGCG CCCTTTGCTG CTATTCTGCC CTTTCAGCAT 
GGAGGTTCCT CAGCCCCTGT CGAATTTGTG GGTGATTTTA CTGCTCCTGA GTCAACCGGT 
GACCCAGAAA AACCACCTCC TCTACCAGAG AAGAAAAACA AACACATGCT GGCCTACATG 
CAGTTGCTGG AGGACTACTC GGAGCCGCAG CCCTCTATGT TCTACCAGAC GCCACAGAAC 
GAGCACATCT ACCAGCAGAA GAACAAGCTC CTCATGGAGG TATACGGCTT CAGCGACTCC 
TTCAGTGGGG TGGACTCCGT GCAGGAGCTG GCCCCGCCGC CCGCCCTACC CCCCAAGCAG 
CGGCAGCTGC AGGCCTCCTG TGCTGCTTCT TCCTTCTCCT CTGTCTCCCA CTGTGTCCAG 
CAAACTAAAG TGGCCTTCAC CCCCGAGGAC GGCAGTGCCG CTCAGGGCCT CAGTGTGTCC 
GTCTCTAACT CCTTTCTCAG CCGGCACGGC AGCTTGCCCG TGCCCTCGTA TAAGTCTGTG 
TTTAGGTCCT ACTCCCAGGA TTTCGTGCCT CACCACCAAG CTTCCGTTCC GCCTTTCCTT 
CCGCCTACCT CCTCTTCCTC TCCACATTTC CCACCTGCCC ACCAGTCTCA GAGCTCTGAC 
TTAGCCGTGC CAACCATGGC CGGTCCACCT CCCAGCACCG TGGACGGGCC TCTCTCGGCT 
TCTCAGGAGA GCAGCTTTCA TGGGAATACT GTCTGCCTTC CTTCCGAAAC CTCTTTCACT 
GACTCGAGTG AAAACGCCAG TGAGGAAGCT GGTGAGGGTG AATATGTCAA TCTGTATTCC 
TCTGGCCAGA GCAGCGAGGA GCTGGCTCCC TCTCGAGGAG AGCCACCGGC TGGGAAAGAC 
GGACATCCCA GAGATCCCTC AGCGGTCAGC GGCGTCCCTG GGAAGGACAG CAGAGACGGC 
AGTGAGAGGG CCCCAAAGTC ACCAGATGCT CTGGAGTCGG CTCAGTCGGA GGAGGAAGTG 
GACGAGCTGT CCCTCATTGA CCACAACGAA ATTATGTCCA GGCTGACGCT CAAGCAGGAG 
GGTGATGACG GGCCGGACGT CCGCGGAGGA TCTGGGGACA TCTTACTGGT CCATGCTACT 
GAGACTGACA GGAAAGATTT GGTGTTGTAC TGCGAGGCAT TCCTGACCAC CTACAGGACC 
TTCATCTCCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA ATTCTCTCCC 
TTTGCCGACA CATTCAAGAA GCGCGTCAGC AAGAACACGT TCTTCGTGCT GGTACGGGTG 
GTGGATGAGC TCTGCCTGGT GGAGTTGACA GAAGAGATCC TGAAGCTGCT GATGGAACTG 
GTCTTCCGCC TGGTGTGCAA TGGGGAGCTG AGCCTGGCCC GTGTGCTCCG GAAGAACATC 
CTGGACAAGG TGGACCAGAA GAAGCTACTC AGGTGTGCCA CCTCCAGCCA GCCCCTGGCA 
GCCCGGGGGG TAGCAGCCAG GCCGGGGACC TTGCACGACT TTCACAGCCA TGAGATAGCG 
GAGCAGCTAA CGCTGCTGGA TGCTGAGCTC TTCTATAAAA TAGAGATTCC TGAGGTTTTG 
CTTTGGGCAA AAGAGCAGAA TGAGGAGAAG AGCCCCAACT TGACCCAGTT CACGGAGCAC 
TTCAACAACA TGTCCTACTG GGTCCGGTCC ATAATCATGT TACAGGAAAA GGCCCAGGAC 
AGGGAACGGC TGCTCTTGAA GTTCATCAAG ATCATGAAGC ACTTGCGGAA GCTGAATAAC 
TTCAACTCCT ACTTGGCCAT CCTCTCTGCC CTGGACTCGG CGCCCATCCG CAGGCTGGAG 
TGGCAGAAGC AGACTTCAGA GGGCCTGGCC GAGTACTGCA CACTGATCGA CAGCTCGTCC 
TCCTTCCGAG CCTACCGGGC CGCCCTCTCG GAGGTGGAAC CGCCGTGCAT CCCGTACCTG 
GGGCTGATCC TGCAGGACCT GACCTTCGTT CACCTGGGAA ACCCAGACTA CATCGACGGG 
AAAGTGAACT TCTCCAAGCG GTGGCAGCAG TTCAACATCC TCGACAGCAT GCGCTGCTTC 
CAGCAGGCGC ACTATGACAT GCGGAGGAAC GACGACATTA TAAACTTCTT CAATGACTTC 
AGTGACCACC TGGCTGAGGA GGCCCTATGG GAACTGTCTC TGAAAATTAA ACCCAGGAAC 
ATAACAAGGA GAAAAACAGA CCGGGAAGAG AAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518573.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516875.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu58060
Clone ID Related Accession (Same CDS sequence)	XM_011518573.3 , XM_011518573.2
Accession Version	XM_011518573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAATCCAGGT TTCCTCTCTT CCAGGAGGCA 
ACAGACAGAT TTCTACCAGA GGGCTACCCT CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA 
GAATTTATGT CCACCAGTGC TGTGGCTTCC AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG 
CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT GCCCTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGATAAGAA GGTACTGGAG ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG 
GAGGCCATCT TACCCCTGGT GCAGAACGAT CCTCGAATTC AGCACAGCTC AGCCCTCTCT 
TCCTGCTATA GCCGAGTGTA CCAAAGCCTC GCCAACCTCA TTCGCTGGTC TGACCAAGTG 
ATGCTGGAAG GCGTGAACTC AGAAGACAAG GAGATGGTGA CGACTGTGAA GGGGGTCATC 
AAGGCTGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCATCGAGAA GCAGGGACGT 
CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAG ATCCTAAACA AGACGACTGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA TGCCACGGAT GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCGCCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGATG TTGACTGTTA CGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTC TTGGCCCTCC TTTCCAGCTG 
CCTCTTGGCG GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGATACG 
CCACCTGCTC TCCCCGAGAA GAAGCGCAGG AGCGCAGCCT CCCAGACGGC GGACGGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTATG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CAGCCCCGAT CCCATCCGTC CCCTACGCGC CCTTTGCTGC TATTCTGCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAG 
TCAACCGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGTTGCTGGA GGACTACTCG GAGCCGCAGC CCTCTATGTT CTACCAGACG 
CCACAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGTGGGGT GGACTCCGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCCAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCACGGCA GCTTGCCCGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AACCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCCT CTCGAGGAGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516875.1
CDS	223..3990
Translation	MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518573.3

ORF Insert Sequence:

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAATCCAGGT TTCCTCTCTT CCAGGAGGCA 
ACAGACAGAT TTCTACCAGA GGGCTACCCT CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA 
GAATTTATGT CCACCAGTGC TGTGGCTTCC AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG 
CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT GCCCTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGATAAGAA GGTACTGGAG ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG 
GAGGCCATCT TACCCCTGGT GCAGAACGAT CCTCGAATTC AGCACAGCTC AGCCCTCTCT 
TCCTGCTATA GCCGAGTGTA CCAAAGCCTC GCCAACCTCA TTCGCTGGTC TGACCAAGTG 
ATGCTGGAAG GCGTGAACTC AGAAGACAAG GAGATGGTGA CGACTGTGAA GGGGGTCATC 
AAGGCTGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCATCGAGAA GCAGGGACGT 
CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAG ATCCTAAACA AGACGACTGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA TGCCACGGAT GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCGCCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGATG TTGACTGTTA CGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTC TTGGCCCTCC TTTCCAGCTG 
CCTCTTGGCG GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGATACG 
CCACCTGCTC TCCCCGAGAA GAAGCGCAGG AGCGCAGCCT CCCAGACGGC GGACGGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTATG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CAGCCCCGAT CCCATCCGTC CCCTACGCGC CCTTTGCTGC TATTCTGCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAG 
TCAACCGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGTTGCTGGA GGACTACTCG GAGCCGCAGC CCTCTATGTT CTACCAGACG 
CCACAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGTGGGGT GGACTCCGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCCAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCACGGCA GCTTGCCCGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AACCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCCT CTCGAGGAGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518573.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516875.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

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CloneID	OHu58060
Clone ID Related Accession (Same CDS sequence)	XM_011518573.3 , XM_011518573.2
Accession Version	XM_011518573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAATCCAGGT TTCCTCTCTT CCAGGAGGCA 
ACAGACAGAT TTCTACCAGA GGGCTACCCT CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA 
GAATTTATGT CCACCAGTGC TGTGGCTTCC AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG 
CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT GCCCTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGATAAGAA GGTACTGGAG ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG 
GAGGCCATCT TACCCCTGGT GCAGAACGAT CCTCGAATTC AGCACAGCTC AGCCCTCTCT 
TCCTGCTATA GCCGAGTGTA CCAAAGCCTC GCCAACCTCA TTCGCTGGTC TGACCAAGTG 
ATGCTGGAAG GCGTGAACTC AGAAGACAAG GAGATGGTGA CGACTGTGAA GGGGGTCATC 
AAGGCTGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCATCGAGAA GCAGGGACGT 
CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAG ATCCTAAACA AGACGACTGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA TGCCACGGAT GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCGCCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGATG TTGACTGTTA CGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTC TTGGCCCTCC TTTCCAGCTG 
CCTCTTGGCG GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGATACG 
CCACCTGCTC TCCCCGAGAA GAAGCGCAGG AGCGCAGCCT CCCAGACGGC GGACGGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTATG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CAGCCCCGAT CCCATCCGTC CCCTACGCGC CCTTTGCTGC TATTCTGCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAG 
TCAACCGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGTTGCTGGA GGACTACTCG GAGCCGCAGC CCTCTATGTT CTACCAGACG 
CCACAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGTGGGGT GGACTCCGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCCAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCACGGCA GCTTGCCCGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AACCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCCT CTCGAGGAGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516875.1
CDS	225..3992
Translation	MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518573.2

ORF Insert Sequence:

ATGGACACAG ACTCTCAGCG TTCTCATCTC TCTTCCTTCA CCATGAAGCT GATGGACAAA 
TTCCACTCAC CCAAAATCAA GAGAACGCCA TCAAAGAAGG GAAAACCAGC TGAGGTGTCC 
GTAAAGATTC CAGAGAAGCC TGTGAACAAA GAATCCAGGT TTCCTCTCTT CCAGGAGGCA 
ACAGACAGAT TTCTACCAGA GGGCTACCCT CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA 
GAATTTATGT CCACCAGTGC TGTGGCTTCC AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG 
CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT GCCCTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGATAAGAA GGTACTGGAG ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG 
GAGGCCATCT TACCCCTGGT GCAGAACGAT CCTCGAATTC AGCACAGCTC AGCCCTCTCT 
TCCTGCTATA GCCGAGTGTA CCAAAGCCTC GCCAACCTCA TTCGCTGGTC TGACCAAGTG 
ATGCTGGAAG GCGTGAACTC AGAAGACAAG GAGATGGTGA CGACTGTGAA GGGGGTCATC 
AAGGCTGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCATCGAGAA GCAGGGACGT 
CCGTCTCCGA CGAGCCCCGT GAAGCCCAGT TCCCCTGCCA GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAG ATCCTAAACA AGACGACTGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA TGCCACGGAT GAAGAGGTCG CGCCCCCCAA GCCTCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCGCCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGATG TTGACTGTTA CGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTC TTGGCCCTCC TTTCCAGCTG 
CCTCTTGGCG GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGATACG 
CCACCTGCTC TCCCCGAGAA GAAGCGCAGG AGCGCAGCCT CCCAGACGGC GGACGGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTATG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CAGCCCCGAT CCCATCCGTC CCCTACGCGC CCTTTGCTGC TATTCTGCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAG 
TCAACCGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGTTGCTGGA GGACTACTCG GAGCCGCAGC CCTCTATGTT CTACCAGACG 
CCACAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGTGGGGT GGACTCCGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCCAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCACGGCA GCTTGCCCGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AACCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCCT CTCGAGGAGA GCCACCGGCT 
GGGAAAGACG GACATCCCAG AGATCCCTCA GCGGTCAGCG GCGTCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATTGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGACGTC CGCGGAGGAT CTGGGGACAT CTTACTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCATT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAA 
TTCTCTCCCT TTGCCGACAC ATTCAAGAAG CGCGTCAGCA AGAACACGTT CTTCGTGCTG 
GTACGGGTGG TGGATGAGCT CTGCCTGGTG GAGTTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAT GGGGAGCTGA GCCTGGCCCG TGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTACTCA GGTGTGCCAC CTCCAGCCAG 
CCCCTGGCAG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATAGCGG AGCAGCTAAC GCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAGAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTGAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCGGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACTTCAGAG GGCCTGGCCG AGTACTGCAC ACTGATCGAC 
AGCTCGTCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGACGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGCTGCTTCC AGCAGGCGCA CTATGACATG CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTGTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518574.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516876.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu58061
Clone ID Related Accession (Same CDS sequence)	XM_011518574.3 , XM_011518574.2
Accession Version	XM_011518574.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516876.1
CDS	177..3920
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518574.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518574.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518574.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516876.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu58061
Clone ID Related Accession (Same CDS sequence)	XM_011518574.3 , XM_011518574.2
Accession Version	XM_011518574.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516876.1
CDS	176..3919
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518574.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518574.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGTATA AGTCTGTGTT TAGGTCCTAC TCCCAGGATT TCGTGCCTCA CCACCAAGCT 
TCCGTTCCGC CTTTCCTTCC GCCTACCTCC TCTTCCTCTC CACATTTCCC ACCTGCCCAC 
CAGTCTCAGA GCTCTGACTT AGCCGTGCCA ACCATGGCCG GTCCACCTCC CAGCACCGTG 
GACGGGCCTC TCTCGGCTTC TCAGGAGAGC AGCTTTCATG GGAATACTGT CTGCCTTCCT 
TCCGAAACCT CTTTCACTGA CTCGGAGCCA CCGGCTGGGA AAGACGGACA TCCCAGAGAT 
CCCTCAGCGG TCAGCGGCGT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATTGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GACGTCCGCG GAGGATCTGG GGACATCTTA CTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCATTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAT GAGAAATTCT CTCCCTTTGC CGACACATTC 
AAGAAGCGCG TCAGCAAGAA CACGTTCTTC GTGCTGGTAC GGGTGGTGGA TGAGCTCTGC 
CTGGTGGAGT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAATGGGG AGCTGAGCCT GGCCCGTGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TACTCAGGTG TGCCACCTCC AGCCAGCCCC TGGCAGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TAGCGGAGCA GCTAACGCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AGAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTGAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCGGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACT 
TCAGAGGGCC TGGCCGAGTA CTGCACACTG ATCGACAGCT CGTCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ACGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGCT GCTTCCAGCA GGCGCACTAT 
GACATGCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT GTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518575.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516877.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

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CloneID	OHu58062
Clone ID Related Accession (Same CDS sequence)	XM_011518575.2 , XM_011518575.3
Accession Version	XM_011518575.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516877.1
CDS	176..3916
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518575.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518575.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518575.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516877.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu58062
Clone ID Related Accession (Same CDS sequence)	XM_011518575.2 , XM_011518575.3
Accession Version	XM_011518575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516877.1
CDS	177..3917
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518575.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518575.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGTATAAGT CTGTGTTTAG GTCCTACTCC CAGGATTTCG TGCCTCACCA CCAAGCTTCC 
GTTCCGCCTT TCCTTCCGCC TACCTCCTCT TCCTCTCCAC ATTTCCCACC TGCCCACCAG 
TCTCAGAGCT CTGACTTAGC CGTGCCAACC ATGGCCGGTC CACCTCCCAG CACCGTGGAC 
GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC TTTCATGGGA ATACTGTCTG CCTTCCTTCC 
GAAACCTCTT TCACTGACTC GGAGCCACCG GCTGGGAAAG ACGGACATCC CAGAGATCCC 
TCAGCGGTCA GCGGCGTCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATT 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAC 
GTCCGCGGAG GATCTGGGGA CATCTTACTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC ATTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAATTCTCTC CCTTTGCCGA CACATTCAAG 
AAGCGCGTCA GCAAGAACAC GTTCTTCGTG CTGGTACGGG TGGTGGATGA GCTCTGCCTG 
GTGGAGTTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AATGGGGAGC TGAGCCTGGC CCGTGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTAC TCAGGTGTGC CACCTCCAGC CAGCCCCTGG CAGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATAG CGGAGCAGCT AACGCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAGA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTG 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC GGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACTTCA 
GAGGGCCTGG CCGAGTACTG CACACTGATC GACAGCTCGT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGACG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGCTGCT TCCAGCAGGC GCACTATGAC 
ATGCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTGTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518576.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516878.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

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CloneID	OHu58063
Clone ID Related Accession (Same CDS sequence)	XM_011518576.2 , XM_011518576.3
Accession Version	XM_011518576.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516878.1
CDS	176..3661
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518576.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518576.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518576.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516878.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

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CloneID	OHu58063
Clone ID Related Accession (Same CDS sequence)	XM_011518576.2 , XM_011518576.3
Accession Version	XM_011518576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516878.1
CDS	177..3662
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518576.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518576.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGCAG GCCTCCTGTG CTGCTTCTTC CTTCTCCTCT 
GTCTCCCACT GTGTCCAGCA AACTAAAGTG GCCTTCACCC CCGAGGACGG CAGTGCCGCT 
CAGGGCCTCA GTGTGTCCGT CTCTAACTCC TTTCTCAGCC GGCACGGCAG CTTGCCCGTG 
CCCTCGGAGC CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC 
GTCCCTGGGA AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG 
GAGTCGGCTC AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT 
ATGTCCAGGC TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT 
GGGGACATCT TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC 
GAGGCATTCC TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG 
AACACGTTCT TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA 
GAGATCCTGA AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC 
CTGGCCCGTG TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG 
TGTGCCACCT CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG 
CACGACTTTC ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC 
TATAAAATAG AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC 
CCCAACTTGA CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA 
ATCATGTTAC AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC 
ATGAAGCACT TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG 
GACTCGGCGC CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG 
TACTGCACAC TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG 
GTGGAACCGC CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC 
CTGGGAAACC CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC 
AACATCCTCG ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC 
GACATTATAA ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA 
CTGTCTCTGA AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518577.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516879.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu58064
Clone ID Related Accession (Same CDS sequence)	XM_011518577.3 , XM_011518577.2
Accession Version	XM_011518577.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516879.1
CDS	177..3659
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518577.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518577.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518577.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516879.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

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CloneID	OHu58064
Clone ID Related Accession (Same CDS sequence)	XM_011518577.3 , XM_011518577.2
Accession Version	XM_011518577.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516879.1
CDS	176..3658
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518577.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518577.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGCC TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC 
TCCCACTGTG TCCAGCAAAC TAAAGTGGCC TTCACCCCCG AGGACGGCAG TGCCGCTCAG 
GGCCTCAGTG TGTCCGTCTC TAACTCCTTT CTCAGCCGGC ACGGCAGCTT GCCCGTGCCC 
TCGGAGCCAC CGGCTGGGAA AGACGGACAT CCCAGAGATC CCTCAGCGGT CAGCGGCGTC 
CCTGGGAAGG ACAGCAGAGA CGGCAGTGAG AGGGCCCCAA AGTCACCAGA TGCTCTGGAG 
TCGGCTCAGT CGGAGGAGGA AGTGGACGAG CTGTCCCTCA TTGACCACAA CGAAATTATG 
TCCAGGCTGA CGCTCAAGCA GGAGGGTGAT GACGGGCCGG ACGTCCGCGG AGGATCTGGG 
GACATCTTAC TGGTCCATGC TACTGAGACT GACAGGAAAG ATTTGGTGTT GTACTGCGAG 
GCATTCCTGA CCACCTACAG GACCTTCATC TCCCCAGAGG AGCTCATCAA GAAGCTGCAG 
TACAGATATG AGAAATTCTC TCCCTTTGCC GACACATTCA AGAAGCGCGT CAGCAAGAAC 
ACGTTCTTCG TGCTGGTACG GGTGGTGGAT GAGCTCTGCC TGGTGGAGTT GACAGAAGAG 
ATCCTGAAGC TGCTGATGGA ACTGGTCTTC CGCCTGGTGT GCAATGGGGA GCTGAGCCTG 
GCCCGTGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT ACTCAGGTGT 
GCCACCTCCA GCCAGCCCCT GGCAGCCCGG GGGGTAGCAG CCAGGCCGGG GACCTTGCAC 
GACTTTCACA GCCATGAGAT AGCGGAGCAG CTAACGCTGC TGGATGCTGA GCTCTTCTAT 
AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAGC AGAATGAGGA GAAGAGCCCC 
AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCCT ACTGGGTCCG GTCCATAATC 
ATGTTACAGG AAAAGGCCCA GGACAGGGAA CGGCTGCTCT TGAAGTTCAT CAAGATCATG 
AAGCACTTGC GGAAGCTGAA TAACTTCAAC TCCTACTTGG CCATCCTCTC TGCCCTGGAC 
TCGGCGCCCA TCCGCAGGCT GGAGTGGCAG AAGCAGACTT CAGAGGGCCT GGCCGAGTAC 
TGCACACTGA TCGACAGCTC GTCCTCCTTC CGAGCCTACC GGGCCGCCCT CTCGGAGGTG 
GAACCGCCGT GCATCCCGTA CCTGGGGCTG ATCCTGCAGG ACCTGACCTT CGTTCACCTG 
GGAAACCCAG ACTACATCGA CGGGAAAGTG AACTTCTCCA AGCGGTGGCA GCAGTTCAAC 
ATCCTCGACA GCATGCGCTG CTTCCAGCAG GCGCACTATG ACATGCGGAG GAACGACGAC 
ATTATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCTG AGGAGGCCCT ATGGGAACTG 
TCTCTGAAAA TTAAACCCAG GAACATAACA AGGAGAAAAA CAGACCGGGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518578.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516880.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

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CloneID	OHu58065
Clone ID Related Accession (Same CDS sequence)	XM_011518578.2 , XM_011518578.3
Accession Version	XM_011518578.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGAGT GAAAACGCCA GTGAGGAAGC TGGTGAGGGT 
GAATATGTCA ATCTGTATTC CTCTGGCCAG AGCAGCGAGG AGCTGGCTCC CTCTCGAGGA 
GAGCCACCGG CTGGGAAAGA CGGACATCCC AGAGATCCCT CAGCGGTCAG CGGCGTCCCT 
GGGAAGGACA GCAGAGACGG CAGTGAGAGG GCCCCAAAGT CACCAGATGC TCTGGAGTCG 
GCTCAGTCGG AGGAGGAAGT GGACGAGCTG TCCCTCATTG ACCACAACGA AATTATGTCC 
AGGCTGACGC TCAAGCAGGA GGGTGATGAC GGGCCGGACG TCCGCGGAGG ATCTGGGGAC 
ATCTTACTGG TCCATGCTAC TGAGACTGAC AGGAAAGATT TGGTGTTGTA CTGCGAGGCA 
TTCCTGACCA CCTACAGGAC CTTCATCTCC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AATTCTCTCC CTTTGCCGAC ACATTCAAGA AGCGCGTCAG CAAGAACACG 
TTCTTCGTGC TGGTACGGGT GGTGGATGAG CTCTGCCTGG TGGAGTTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAACT GGTCTTCCGC CTGGTGTGCA ATGGGGAGCT GAGCCTGGCC 
CGTGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTACT CAGGTGTGCC 
ACCTCCAGCC AGCCCCTGGC AGCCCGGGGG GTAGCAGCCA GGCCGGGGAC CTTGCACGAC 
TTTCACAGCC ATGAGATAGC GGAGCAGCTA ACGCTGCTGG ATGCTGAGCT CTTCTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAGCAGA ATGAGGAGAA GAGCCCCAAC 
TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC CATAATCATG 
TTACAGGAAA AGGCCCAGGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTGCGGA AGCTGAATAA CTTCAACTCC TACTTGGCCA TCCTCTCTGC CCTGGACTCG 
GCGCCCATCC GCAGGCTGGA GTGGCAGAAG CAGACTTCAG AGGGCCTGGC CGAGTACTGC 
ACACTGATCG ACAGCTCGTC CTCCTTCCGA GCCTACCGGG CCGCCCTCTC GGAGGTGGAA 
CCGCCGTGCA TCCCGTACCT GGGGCTGATC CTGCAGGACC TGACCTTCGT TCACCTGGGA 
AACCCAGACT ACATCGACGG GAAAGTGAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC 
CTCGACAGCA TGCGCTGCTT CCAGCAGGCG CACTATGACA TGCGGAGGAA CGACGACATT 
ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCTGAGG AGGCCCTATG GGAACTGTCT 
CTGAAAATTA AACCCAGGAA CATAACAAGG AGAAAAACAG ACCGGGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516880.1
CDS	176..3595
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518578.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518578.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGAGT GAAAACGCCA GTGAGGAAGC TGGTGAGGGT 
GAATATGTCA ATCTGTATTC CTCTGGCCAG AGCAGCGAGG AGCTGGCTCC CTCTCGAGGA 
GAGCCACCGG CTGGGAAAGA CGGACATCCC AGAGATCCCT CAGCGGTCAG CGGCGTCCCT 
GGGAAGGACA GCAGAGACGG CAGTGAGAGG GCCCCAAAGT CACCAGATGC TCTGGAGTCG 
GCTCAGTCGG AGGAGGAAGT GGACGAGCTG TCCCTCATTG ACCACAACGA AATTATGTCC 
AGGCTGACGC TCAAGCAGGA GGGTGATGAC GGGCCGGACG TCCGCGGAGG ATCTGGGGAC 
ATCTTACTGG TCCATGCTAC TGAGACTGAC AGGAAAGATT TGGTGTTGTA CTGCGAGGCA 
TTCCTGACCA CCTACAGGAC CTTCATCTCC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AATTCTCTCC CTTTGCCGAC ACATTCAAGA AGCGCGTCAG CAAGAACACG 
TTCTTCGTGC TGGTACGGGT GGTGGATGAG CTCTGCCTGG TGGAGTTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAACT GGTCTTCCGC CTGGTGTGCA ATGGGGAGCT GAGCCTGGCC 
CGTGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTACT CAGGTGTGCC 
ACCTCCAGCC AGCCCCTGGC AGCCCGGGGG GTAGCAGCCA GGCCGGGGAC CTTGCACGAC 
TTTCACAGCC ATGAGATAGC GGAGCAGCTA ACGCTGCTGG ATGCTGAGCT CTTCTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAGCAGA ATGAGGAGAA GAGCCCCAAC 
TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC CATAATCATG 
TTACAGGAAA AGGCCCAGGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTGCGGA AGCTGAATAA CTTCAACTCC TACTTGGCCA TCCTCTCTGC CCTGGACTCG 
GCGCCCATCC GCAGGCTGGA GTGGCAGAAG CAGACTTCAG AGGGCCTGGC CGAGTACTGC 
ACACTGATCG ACAGCTCGTC CTCCTTCCGA GCCTACCGGG CCGCCCTCTC GGAGGTGGAA 
CCGCCGTGCA TCCCGTACCT GGGGCTGATC CTGCAGGACC TGACCTTCGT TCACCTGGGA 
AACCCAGACT ACATCGACGG GAAAGTGAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC 
CTCGACAGCA TGCGCTGCTT CCAGCAGGCG CACTATGACA TGCGGAGGAA CGACGACATT 
ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCTGAGG AGGCCCTATG GGAACTGTCT 
CTGAAAATTA AACCCAGGAA CATAACAAGG AGAAAAACAG ACCGGGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518578.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516880.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu58065
Clone ID Related Accession (Same CDS sequence)	XM_011518578.2 , XM_011518578.3
Accession Version	XM_011518578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGAGT GAAAACGCCA GTGAGGAAGC TGGTGAGGGT 
GAATATGTCA ATCTGTATTC CTCTGGCCAG AGCAGCGAGG AGCTGGCTCC CTCTCGAGGA 
GAGCCACCGG CTGGGAAAGA CGGACATCCC AGAGATCCCT CAGCGGTCAG CGGCGTCCCT 
GGGAAGGACA GCAGAGACGG CAGTGAGAGG GCCCCAAAGT CACCAGATGC TCTGGAGTCG 
GCTCAGTCGG AGGAGGAAGT GGACGAGCTG TCCCTCATTG ACCACAACGA AATTATGTCC 
AGGCTGACGC TCAAGCAGGA GGGTGATGAC GGGCCGGACG TCCGCGGAGG ATCTGGGGAC 
ATCTTACTGG TCCATGCTAC TGAGACTGAC AGGAAAGATT TGGTGTTGTA CTGCGAGGCA 
TTCCTGACCA CCTACAGGAC CTTCATCTCC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AATTCTCTCC CTTTGCCGAC ACATTCAAGA AGCGCGTCAG CAAGAACACG 
TTCTTCGTGC TGGTACGGGT GGTGGATGAG CTCTGCCTGG TGGAGTTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAACT GGTCTTCCGC CTGGTGTGCA ATGGGGAGCT GAGCCTGGCC 
CGTGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTACT CAGGTGTGCC 
ACCTCCAGCC AGCCCCTGGC AGCCCGGGGG GTAGCAGCCA GGCCGGGGAC CTTGCACGAC 
TTTCACAGCC ATGAGATAGC GGAGCAGCTA ACGCTGCTGG ATGCTGAGCT CTTCTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAGCAGA ATGAGGAGAA GAGCCCCAAC 
TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC CATAATCATG 
TTACAGGAAA AGGCCCAGGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTGCGGA AGCTGAATAA CTTCAACTCC TACTTGGCCA TCCTCTCTGC CCTGGACTCG 
GCGCCCATCC GCAGGCTGGA GTGGCAGAAG CAGACTTCAG AGGGCCTGGC CGAGTACTGC 
ACACTGATCG ACAGCTCGTC CTCCTTCCGA GCCTACCGGG CCGCCCTCTC GGAGGTGGAA 
CCGCCGTGCA TCCCGTACCT GGGGCTGATC CTGCAGGACC TGACCTTCGT TCACCTGGGA 
AACCCAGACT ACATCGACGG GAAAGTGAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC 
CTCGACAGCA TGCGCTGCTT CCAGCAGGCG CACTATGACA TGCGGAGGAA CGACGACATT 
ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCTGAGG AGGCCCTATG GGAACTGTCT 
CTGAAAATTA AACCCAGGAA CATAACAAGG AGAAAAACAG ACCGGGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516880.1
CDS	177..3596
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518578.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518578.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGAGT GAAAACGCCA GTGAGGAAGC TGGTGAGGGT 
GAATATGTCA ATCTGTATTC CTCTGGCCAG AGCAGCGAGG AGCTGGCTCC CTCTCGAGGA 
GAGCCACCGG CTGGGAAAGA CGGACATCCC AGAGATCCCT CAGCGGTCAG CGGCGTCCCT 
GGGAAGGACA GCAGAGACGG CAGTGAGAGG GCCCCAAAGT CACCAGATGC TCTGGAGTCG 
GCTCAGTCGG AGGAGGAAGT GGACGAGCTG TCCCTCATTG ACCACAACGA AATTATGTCC 
AGGCTGACGC TCAAGCAGGA GGGTGATGAC GGGCCGGACG TCCGCGGAGG ATCTGGGGAC 
ATCTTACTGG TCCATGCTAC TGAGACTGAC AGGAAAGATT TGGTGTTGTA CTGCGAGGCA 
TTCCTGACCA CCTACAGGAC CTTCATCTCC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AATTCTCTCC CTTTGCCGAC ACATTCAAGA AGCGCGTCAG CAAGAACACG 
TTCTTCGTGC TGGTACGGGT GGTGGATGAG CTCTGCCTGG TGGAGTTGAC AGAAGAGATC 
CTGAAGCTGC TGATGGAACT GGTCTTCCGC CTGGTGTGCA ATGGGGAGCT GAGCCTGGCC 
CGTGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTACT CAGGTGTGCC 
ACCTCCAGCC AGCCCCTGGC AGCCCGGGGG GTAGCAGCCA GGCCGGGGAC CTTGCACGAC 
TTTCACAGCC ATGAGATAGC GGAGCAGCTA ACGCTGCTGG ATGCTGAGCT CTTCTATAAA 
ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAGCAGA ATGAGGAGAA GAGCCCCAAC 
TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCCTACT GGGTCCGGTC CATAATCATG 
TTACAGGAAA AGGCCCAGGA CAGGGAACGG CTGCTCTTGA AGTTCATCAA GATCATGAAG 
CACTTGCGGA AGCTGAATAA CTTCAACTCC TACTTGGCCA TCCTCTCTGC CCTGGACTCG 
GCGCCCATCC GCAGGCTGGA GTGGCAGAAG CAGACTTCAG AGGGCCTGGC CGAGTACTGC 
ACACTGATCG ACAGCTCGTC CTCCTTCCGA GCCTACCGGG CCGCCCTCTC GGAGGTGGAA 
CCGCCGTGCA TCCCGTACCT GGGGCTGATC CTGCAGGACC TGACCTTCGT TCACCTGGGA 
AACCCAGACT ACATCGACGG GAAAGTGAAC TTCTCCAAGC GGTGGCAGCA GTTCAACATC 
CTCGACAGCA TGCGCTGCTT CCAGCAGGCG CACTATGACA TGCGGAGGAA CGACGACATT 
ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCTGAGG AGGCCCTATG GGAACTGTCT 
CTGAAAATTA AACCCAGGAA CATAACAAGG AGAAAAACAG ACCGGGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518579.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516881.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu58066
Clone ID Related Accession (Same CDS sequence)	XM_011518579.2 , XM_011518579.3
Accession Version	XM_011518579.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATTGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGACGTCC GCGGAGGATC TGGGGACATC TTACTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCATTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAAT TCTCTCCCTT TGCCGACACA 
TTCAAGAAGC GCGTCAGCAA GAACACGTTC TTCGTGCTGG TACGGGTGGT GGATGAGCTC 
TGCCTGGTGG AGTTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAATG GGGAGCTGAG CCTGGCCCGT GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTACTCAG GTGTGCCACC TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATAGCGGA GCAGCTAACG 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAGAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCGGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACTTCAGAGG GCCTGGCCGA GTACTGCACA CTGATCGACA GCTCGTCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGACGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GCTGCTTCCA GCAGGCGCAC 
TATGACATGC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTGTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516881.1
CDS	176..3502
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518579.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518579.2

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATTGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGACGTCC GCGGAGGATC TGGGGACATC TTACTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCATTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAAT TCTCTCCCTT TGCCGACACA 
TTCAAGAAGC GCGTCAGCAA GAACACGTTC TTCGTGCTGG TACGGGTGGT GGATGAGCTC 
TGCCTGGTGG AGTTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAATG GGGAGCTGAG CCTGGCCCGT GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTACTCAG GTGTGCCACC TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATAGCGGA GCAGCTAACG 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAGAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCGGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACTTCAGAGG GCCTGGCCGA GTACTGCACA CTGATCGACA GCTCGTCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGACGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GCTGCTTCCA GCAGGCGCAC 
TATGACATGC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTGTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_011518579.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516881.1 Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu58066
Clone ID Related Accession (Same CDS sequence)	XM_011518579.2 , XM_011518579.3
Accession Version	XM_011518579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATTGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGACGTCC GCGGAGGATC TGGGGACATC TTACTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCATTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAAT TCTCTCCCTT TGCCGACACA 
TTCAAGAAGC GCGTCAGCAA GAACACGTTC TTCGTGCTGG TACGGGTGGT GGATGAGCTC 
TGCCTGGTGG AGTTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAATG GGGAGCTGAG CCTGGCCCGT GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTACTCAG GTGTGCCACC TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATAGCGGA GCAGCTAACG 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAGAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCGGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACTTCAGAGG GCCTGGCCGA GTACTGCACA CTGATCGACA GCTCGTCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGACGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GCTGCTTCCA GCAGGCGCAC 
TATGACATGC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTGTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516881.1
CDS	177..3503
Translation	MPISFLGFRPSLTTLEQNKRTGGVVPDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKESRFPLFQEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_011518579.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518579.3

ORF Insert Sequence:

ATGCCCATTT CTTTCTTGGG ATTTCGTCCC AGCCTGACAA CCTTGGAACA GAACAAGAGG 
ACAGGAGGTG TCGTTCCAGA CTCTCAGCGT TCTCATCTCT CTTCCTTCAC CATGAAGCTG 
ATGGACAAAT TCCACTCACC CAAAATCAAG AGAACGCCAT CAAAGAAGGG AAAACCAGCT 
GAGGTGTCCG TAAAGATTCC AGAGAAGCCT GTGAACAAAG AATCCAGGTT TCCTCTCTTC 
CAGGAGGCAA CAGACAGATT TCTACCAGAG GGCTACCCTC TCCCCTTGGA TCTGGAGCAG 
CAGGCAGTAG AATTTATGTC CACCAGTGCT GTGGCTTCCA GGTCTCAAAG GCAGAAGAAC 
CTGAGCTGGC TGGAGGAGAA AGAGAAGGAA GTTGTCAGTG CCCTGCGCTA CTTTAAGACC 
ATTGTGGACA AAATGGCAAT TGATAAGAAG GTACTGGAGA TGCTTCCAGG GTCAGCCAGC 
AAGGTGCTGG AGGCCATCTT ACCCCTGGTG CAGAACGATC CTCGAATTCA GCACAGCTCA 
GCCCTCTCTT CCTGCTATAG CCGAGTGTAC CAAAGCCTCG CCAACCTCAT TCGCTGGTCT 
GACCAAGTGA TGCTGGAAGG CGTGAACTCA GAAGACAAGG AGATGGTGAC GACTGTGAAG 
GGGGTCATCA AGGCTGTGCT GGATGGAGTG AAGGAGCTGG TCAGGCTCAC CATCGAGAAG 
CAGGGACGTC CGTCTCCGAC GAGCCCCGTG AAGCCCAGTT CCCCTGCCAG CAAGCCTGAT 
GGCCCAGCAG AGCTCCCCCT GACAGACCGC GAGGTAGAGA TCCTAAACAA GACGACTGGG 
ATGTCACAGT CAACTGAGCT CCTCCCAGAT GCCACGGATG AAGAGGTCGC GCCCCCCAAG 
CCTCCTCTGC CTGGCATTCG GGTGGTTGAT AATAGTCCTC CACCAGCATT GCCACCCAAG 
AAAAGACAGT CGGCGCCGTC CCCTACCCGA GTGGCTGTGG TGGCCCCCAT GAGCCGAGCC 
ACCAGTGGCT CCAGTTTGCC TGTTGGAATC AATAGGCAGG ATTTTGATGT TGACTGTTAC 
GCACAGAGGC GACTGTCAGG AGGCAGCCAC TCATATGGTG GAGAGTCGCC CCGCCTCTCC 
CCCTGCAGCA GCATAGGCAA GCTCAGCAAG TCAGACGAGC AGCTGTCCTC TCTGGACAGG 
GACAGTGGGC AGTGCTCCCG GAACACAAGC TGTGAAACAC TAGACCACTA TGATCCCGAC 
TATGAATTCC TCCAGCAAGA CCTCTCTAAC GCAGACCAGA TACCTCAGCA GACGGCCTGG 
AACCTTAGCC CGTTGCCAGA GTCTTTGGGG GAGTCTGGGT CTCCATTTCT TGGCCCTCCT 
TTCCAGCTGC CTCTTGGCGG CCATCCCCAG CCAGACGGAC CTCTGGCCCC AGGGCAGCAG 
ACAGATACGC CACCTGCTCT CCCCGAGAAG AAGCGCAGGA GCGCAGCCTC CCAGACGGCG 
GACGGCTCTG GCTGCAGGGT GTCCTACGAG CGGCATCCCT CGCAGTATGA CAACATCTCT 
GGGGAGGACC TGCAGAGCAC AGCCCCGATC CCATCCGTCC CCTACGCGCC CTTTGCTGCT 
ATTCTGCCCT TTCAGCATGG AGGTTCCTCA GCCCCTGTCG AATTTGTGGG TGATTTTACT 
GCTCCTGAGT CAACCGGTGA CCCAGAAAAA CCACCTCCTC TACCAGAGAA GAAAAACAAA 
CACATGCTGG CCTACATGCA GTTGCTGGAG GACTACTCGG AGCCGCAGCC CTCTATGTTC 
TACCAGACGC CACAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTA 
TACGGCTTCA GCGACTCCTT CAGTGGGGTG GACTCCGTGC AGGAGCTGGC CCCGCCGCCC 
GCCCTACCCC CCAAGCAGCG GCAGCTGGAG CCACCGGCTG GGAAAGACGG ACATCCCAGA 
GATCCCTCAG CGGTCAGCGG CGTCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATTGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGACGTCC GCGGAGGATC TGGGGACATC TTACTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCATTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAAT TCTCTCCCTT TGCCGACACA 
TTCAAGAAGC GCGTCAGCAA GAACACGTTC TTCGTGCTGG TACGGGTGGT GGATGAGCTC 
TGCCTGGTGG AGTTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAATG GGGAGCTGAG CCTGGCCCGT GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTACTCAG GTGTGCCACC TCCAGCCAGC CCCTGGCAGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATAGCGGA GCAGCTAACG 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAGAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTGAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCGGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACTTCAGAGG GCCTGGCCGA GTACTGCACA CTGATCGACA GCTCGTCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGACGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GCTGCTTCCA GCAGGCGCAC 
TATGACATGC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTGTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C3G knock-down enhances migration and invasion by increasing Rap1-mediated p38? activation, while it impairs tumor growth through p38?-independent mechanisms.
Oncotarget7(29)45060-45078(2016 Jul)
Priego N,Arechederra M,Sequera C,Bragado P,V?zquez-Carballo A,Guti?rrez-Uzquiza ?,Mart?n-Granado V,Ventura JJ,Kazanietz MG,Guerrero C,Porras A

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

The C3G/Rap1 pathway promotes secretion of MMP-2 and MMP-9 and is involved in serous ovarian cancer metastasis.
Cancer letters359(2)241-9(2015 Apr)
Che YL,Luo SJ,Li G,Cheng M,Gao YM,Li XM,Dai JM,He H,Wang J,Peng HJ,Zhang Y,Li WY,Wang H,Liu B,Linghu H

C3G transgenic mouse models with specific expression in platelets reveal a new role for C3G in platelet clotting through its GEF activity.
Biochimica et biophysica acta1823(8)1366-77(2012 Aug)
Guti?rrez-Herrero S,Maia V,Guti?rrez-Berzal J,Calzada N,Sanz M,Gonz?lez-Manch?n C,Pericacho M,Ortiz-Rivero S,Gonz?lez-Porras JR,Arechederra M,Porras A,Guerrero C

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RAPGEF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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