RAPGEF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following RAPGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe38648 XM_014116986.1
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe12665 XM_005625210.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12666 XM_005625211.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12667 XM_005625212.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12668 XM_005625213.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12669 XM_005625214.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12670 XM_005625215.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12671 XM_005625216.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12673 XM_005625218.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe12674 XM_005625219.3
Latest version!
Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe12665 XM_005625210.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12667 XM_005625212.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12666 XM_005625211.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12668 XM_005625213.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12669 XM_005625214.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12674 XM_005625219.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe12670 XM_005625215.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe12673 XM_005625218.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe12671 XM_005625216.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe38648
    Clone ID Related Accession (Same CDS sequence) XM_014116986.1
    Accession Version XM_014116986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGGTGGG CTGGTGAAGA AAAGAACAGC AGACTTGCAG ATTCTCAGCG TTCTCACCTC 
    TCTTCCTTCA CCATGAAGCT GAAGGACAAA TTCCACTCAC CCAAAATCAA GAGAACGCCA
    TCAAAGAAGG GAAAACCAGC TGAAGTTTCT GTGAAAAATC CGGAGAAGCC TGTGAGCAAA
    GAGGCAACAG ACAGATTTCT ACCAGAGGGC TACCCTCTCC CCTTGGATCT GGAGCAGCAG
    GCAGTAGAAT TTATGTCCAC CAGTGCTGTG GCTTCCAGGT CTCAGAGGCA GAAGAACCTG
    AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT GTTAGTGCCT TGCGCTACTT TAAGACTATC
    GTGGACAAGA TGGCTATTGA TAAGAAGGTA CTGGAGATGC TGCCAGGGTC AGCCAGCAAG
    GTGCTGGAGG CCATCTTACC CCTGGTGCAG AGCGATCCTC GAATTCAACA CAGCTCAGCC
    CTCTCCTCCT GCTATAGCCG AGTGTACCAA AGTCTTGCCA ACCTCATCCG TTGGTCTGAC
    CAAGTGATGC TGGAAGGTGT GAACTCAGAA GATAAAGAGA CGGTGACAAC GGTGAAGGGG
    GTCATCAAGG CGGTACTGGA TGGAGTGAAG GAGCTGGTCA GACTTACCAT CGAGAAACAG
    GGGCATCCAT CTCCAACCAG CCCAGTGAAA CCCAGTTCCC CAGCCTGCAA ACCGGACGGC
    CAGTCTGAGC TCCCCCTGAC AGATCGCGAG GTGGAGATTC TCAACAAGAC AACTGGCATG
    TCCCAGTCGG CCGAGCTACT CCCAGACTCA ACAGATGAGG AGGTTGCGCC CCCTAAGCCC
    CCCTTACCTG GCATTCGGGT GGTTGATAAC AGTCCTCCCC CAGCATTGCC ACCGAAGAAA
    AGACAGTCAG CTCCATCCCC GACCCGAGTG GCTGTTGTTG CCCCCATGAG TCGAGCCACC
    AGTGGCTCCA GTTTGCCTGT TGGAATTAAT AGGCAGGATT TTGATGTGGA CTGTTACACA
    CAGAGGCGAC TCTCAGGAGG CAGCCACTCA TACGGCGGAG AGTCGCCCCG TCTGTCCCCC
    TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA GATGAGCAGC TGTCCTCTCT GGACAGGGAC
    AGCGGCCAGT GCTCCCGGAA CACAAGCTGT GAAACACTAG ATCATTATGA TCCTGACTAC
    GAATTCCTTC AGCAAGACCT CTCTAATGCA GACCAGATAC CTCAGCAAGT GGCCTGTAAC
    CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG TCTGGGTCTC CATTTCTTGG CCATCCTTTC
    CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC CAGCCAGAGG GGCCCTTGGC CCCAGGACAG
    CAGATAGACA TGCCACCTGC TCTCCCTGAG AAGAAGCGCA GGAGTGCGGC CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GATGTCCTAC GAGCGGCACC CCTCACAATA TGACAATATC
    TCGGAAGATG ACCTGCAGAA CCCAGCCTCG GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT
    GCTATTCTCC CCTTTCAGCA AGGAGGGTCC TCAGCCTCTG TCGAATTTGT GGGTGATTTC
    ACTGTTTCTG AATCAACAAC GGGTGACCCA GAAAAACCAC CTCCTCTACC AGAGAAGAAA
    AACAAACACA TGCTGGCCTA CATGCAGCTG CTGGAGGACT ACTCGGAGCC ACAGCCGTCC
    ATGTTCTACC AGACGCCACA GAACGAGCAC ATCTACCAGC AGAAGAACAA GCTCCTCATG
    GAGGTGTACG GCTTCAACGA CTCATTCAGC ACCGAAGCCC CGCAGGAGCT GGCACCGCCC
    CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC
    TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC
    ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC TCCTTTCTCA GCCGGCATGG CAACTTGCCT
    GTGCCCTCGT ATAAATCTGT GTTTAGGTCC TACTCCCAGG ATTTCGTGCC TCACAACCAC
    GCTTCCGTTC AGCCTTTCCT TCCGCCTACC TCCTCTGCCT CTCCACACTT CCCAACTGTC
    CACCAGTCTC AGAGCTCTGA CTTAACGGTG CCAACCGTAG CCAGTCTGCC TCCCAGCACC
    GTGGACGGGC CTCTCTCGTC TTCTCAGGAG AGCAGCTTTC ACGGGAACAC TGTCTGCCTT
    CCTTCCGAAA CCTCTTTCAC TGACTCGCCC CAGACGCCTT TGAGTGAAAG CACCAGCGAG
    GAGGCTGGTG AGGGTGAATA CGTCAATCTA TATTCCTCTG GCCAGAGCAG CAGGGAGCTG
    GCTCACTGTG GAGGAGAGTC ACCAGCTATG AAAGATGGAC ATCCCAGAGA CCCCTCATCG
    GTCAGCAGCA CACCTGGGAA GGAGAGCAGA GACAGCGACA GGGCCTCAAG GTCACCAGAT
    GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA GTGGATGAGC TGTCCCTCAT TGACCACAAT
    GAAATTATGG CAAGGCTGAC ACTCAAGCAG GAGGGTGATG ATGGGCCAGA CGTCCGTGGA
    GGGTCTGGAG ATATCCTACT GGTGCACGCT ACTGAGACAG ACAGGAAAGA TTTGGTGTTG
    TACTGTGAGG CCTTCTTGAC CACCTACAGG ACATTCATCA CCCCAGAGGA GCTCATCAAG
    AAGCTGCAGT ACAGGTACGA GAAGTTCTCT CCCTTCGCTG ACACCTTCAA GAAGCGCGTC
    AGCAAGAACA CTTTCTTCGT GCTGGTGCGA GTGGTGGATG AGCTCTGCCT AGTGGAGTTG
    ACAGAAGAGA TCTTGAAGCT GCTCATGGAA CTGGTCTTTC GCTTGGTGTG CAACGGGGAG
    CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC ATCCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCAACTCCGA CCAGCCCCTG GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG
    ACTTTGCATG ACTTTCACAG CCATGAAATA GCCGAGCAGC TGACACTGCT GGATGCTGAG
    CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CAAAAGAACA GAATGAGGAG
    AAAAGCCCCA ACTTGACCCA GTTCACGGAG CACTTCAACA ACATGTCGTA CTGGGTCCGG
    TCAATAATCA TGTTACAAGA AAAAGCCCAG GACAGGGAGC GACTGCTACT GAAGTTCATC
    AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTTAACT CCTACCTGGC CATCCTCTCG
    GCACTGGACT CAGCTCCCAT CCGCAGGCTA GAGTGGCAGA AGCAGACCTC GGAGGGCCTG
    GCCGAGTACT GTACACTGAT CGACAGCTCG TCCTCCTTCC GCGCCTACCG GGCCGCCCTG
    TCCGAGGTGG AGCCGCCCTG CATCCCATAC CTGGGGCTTA TCCTGCAGGA CCTCACCTTT
    GTTCACCTGG GAAACCCAGA CTATATTGAC GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG
    CAGTTCAACA TTCTGGACAG CATGCGGTGC TTCCAACAAG CGCACTATGA TATCCGAAGA
    AACGATGACA TCATAAACTT CTTCAATGAC TTCAGTGACC ACCTGGCGGA GGAGGCTCTG
    TGGGAACTCT CTCTGAAAAT CAAACCTAGG AACATAACAA GGAGAAAAAC AGACCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972461.1
    CDS16..3807
    Translation

    Target ORF information:

    RefSeq Version XM_014116986.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTGGTGGG CTGGTGAAGA AAAGAACAGC AGACTTGCAG ATTCTCAGCG TTCTCACCTC 
    TCTTCCTTCA CCATGAAGCT GAAGGACAAA TTCCACTCAC CCAAAATCAA GAGAACGCCA
    TCAAAGAAGG GAAAACCAGC TGAAGTTTCT GTGAAAAATC CGGAGAAGCC TGTGAGCAAA
    GAGGCAACAG ACAGATTTCT ACCAGAGGGC TACCCTCTCC CCTTGGATCT GGAGCAGCAG
    GCAGTAGAAT TTATGTCCAC CAGTGCTGTG GCTTCCAGGT CTCAGAGGCA GAAGAACCTG
    AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT GTTAGTGCCT TGCGCTACTT TAAGACTATC
    GTGGACAAGA TGGCTATTGA TAAGAAGGTA CTGGAGATGC TGCCAGGGTC AGCCAGCAAG
    GTGCTGGAGG CCATCTTACC CCTGGTGCAG AGCGATCCTC GAATTCAACA CAGCTCAGCC
    CTCTCCTCCT GCTATAGCCG AGTGTACCAA AGTCTTGCCA ACCTCATCCG TTGGTCTGAC
    CAAGTGATGC TGGAAGGTGT GAACTCAGAA GATAAAGAGA CGGTGACAAC GGTGAAGGGG
    GTCATCAAGG CGGTACTGGA TGGAGTGAAG GAGCTGGTCA GACTTACCAT CGAGAAACAG
    GGGCATCCAT CTCCAACCAG CCCAGTGAAA CCCAGTTCCC CAGCCTGCAA ACCGGACGGC
    CAGTCTGAGC TCCCCCTGAC AGATCGCGAG GTGGAGATTC TCAACAAGAC AACTGGCATG
    TCCCAGTCGG CCGAGCTACT CCCAGACTCA ACAGATGAGG AGGTTGCGCC CCCTAAGCCC
    CCCTTACCTG GCATTCGGGT GGTTGATAAC AGTCCTCCCC CAGCATTGCC ACCGAAGAAA
    AGACAGTCAG CTCCATCCCC GACCCGAGTG GCTGTTGTTG CCCCCATGAG TCGAGCCACC
    AGTGGCTCCA GTTTGCCTGT TGGAATTAAT AGGCAGGATT TTGATGTGGA CTGTTACACA
    CAGAGGCGAC TCTCAGGAGG CAGCCACTCA TACGGCGGAG AGTCGCCCCG TCTGTCCCCC
    TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA GATGAGCAGC TGTCCTCTCT GGACAGGGAC
    AGCGGCCAGT GCTCCCGGAA CACAAGCTGT GAAACACTAG ATCATTATGA TCCTGACTAC
    GAATTCCTTC AGCAAGACCT CTCTAATGCA GACCAGATAC CTCAGCAAGT GGCCTGTAAC
    CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG TCTGGGTCTC CATTTCTTGG CCATCCTTTC
    CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC CAGCCAGAGG GGCCCTTGGC CCCAGGACAG
    CAGATAGACA TGCCACCTGC TCTCCCTGAG AAGAAGCGCA GGAGTGCGGC CTCCCAGACC
    ACGGACAGCT CTGGCTGCAG GATGTCCTAC GAGCGGCACC CCTCACAATA TGACAATATC
    TCGGAAGATG ACCTGCAGAA CCCAGCCTCG GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT
    GCTATTCTCC CCTTTCAGCA AGGAGGGTCC TCAGCCTCTG TCGAATTTGT GGGTGATTTC
    ACTGTTTCTG AATCAACAAC GGGTGACCCA GAAAAACCAC CTCCTCTACC AGAGAAGAAA
    AACAAACACA TGCTGGCCTA CATGCAGCTG CTGGAGGACT ACTCGGAGCC ACAGCCGTCC
    ATGTTCTACC AGACGCCACA GAACGAGCAC ATCTACCAGC AGAAGAACAA GCTCCTCATG
    GAGGTGTACG GCTTCAACGA CTCATTCAGC ACCGAAGCCC CGCAGGAGCT GGCACCGCCC
    CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC
    TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC
    ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC TCCTTTCTCA GCCGGCATGG CAACTTGCCT
    GTGCCCTCGT ATAAATCTGT GTTTAGGTCC TACTCCCAGG ATTTCGTGCC TCACAACCAC
    GCTTCCGTTC AGCCTTTCCT TCCGCCTACC TCCTCTGCCT CTCCACACTT CCCAACTGTC
    CACCAGTCTC AGAGCTCTGA CTTAACGGTG CCAACCGTAG CCAGTCTGCC TCCCAGCACC
    GTGGACGGGC CTCTCTCGTC TTCTCAGGAG AGCAGCTTTC ACGGGAACAC TGTCTGCCTT
    CCTTCCGAAA CCTCTTTCAC TGACTCGCCC CAGACGCCTT TGAGTGAAAG CACCAGCGAG
    GAGGCTGGTG AGGGTGAATA CGTCAATCTA TATTCCTCTG GCCAGAGCAG CAGGGAGCTG
    GCTCACTGTG GAGGAGAGTC ACCAGCTATG AAAGATGGAC ATCCCAGAGA CCCCTCATCG
    GTCAGCAGCA CACCTGGGAA GGAGAGCAGA GACAGCGACA GGGCCTCAAG GTCACCAGAT
    GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA GTGGATGAGC TGTCCCTCAT TGACCACAAT
    GAAATTATGG CAAGGCTGAC ACTCAAGCAG GAGGGTGATG ATGGGCCAGA CGTCCGTGGA
    GGGTCTGGAG ATATCCTACT GGTGCACGCT ACTGAGACAG ACAGGAAAGA TTTGGTGTTG
    TACTGTGAGG CCTTCTTGAC CACCTACAGG ACATTCATCA CCCCAGAGGA GCTCATCAAG
    AAGCTGCAGT ACAGGTACGA GAAGTTCTCT CCCTTCGCTG ACACCTTCAA GAAGCGCGTC
    AGCAAGAACA CTTTCTTCGT GCTGGTGCGA GTGGTGGATG AGCTCTGCCT AGTGGAGTTG
    ACAGAAGAGA TCTTGAAGCT GCTCATGGAA CTGGTCTTTC GCTTGGTGTG CAACGGGGAG
    CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC ATCCTGGACA AGGTGGACCA GAAGAAGCTG
    CTCAGGTGTG CCAACTCCGA CCAGCCCCTG GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG
    ACTTTGCATG ACTTTCACAG CCATGAAATA GCCGAGCAGC TGACACTGCT GGATGCTGAG
    CTCTTTTATA AAATAGAGAT TCCTGAGGTT TTGCTTTGGG CAAAAGAACA GAATGAGGAG
    AAAAGCCCCA ACTTGACCCA GTTCACGGAG CACTTCAACA ACATGTCGTA CTGGGTCCGG
    TCAATAATCA TGTTACAAGA AAAAGCCCAG GACAGGGAGC GACTGCTACT GAAGTTCATC
    AAGATCATGA AGCACTTACG GAAGCTGAAC AACTTTAACT CCTACCTGGC CATCCTCTCG
    GCACTGGACT CAGCTCCCAT CCGCAGGCTA GAGTGGCAGA AGCAGACCTC GGAGGGCCTG
    GCCGAGTACT GTACACTGAT CGACAGCTCG TCCTCCTTCC GCGCCTACCG GGCCGCCCTG
    TCCGAGGTGG AGCCGCCCTG CATCCCATAC CTGGGGCTTA TCCTGCAGGA CCTCACCTTT
    GTTCACCTGG GAAACCCAGA CTATATTGAC GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG
    CAGTTCAACA TTCTGGACAG CATGCGGTGC TTCCAACAAG CGCACTATGA TATCCGAAGA
    AACGATGACA TCATAAACTT CTTCAATGAC TTCAGTGACC ACCTGGCGGA GGAGGCTCTG
    TGGGAACTCT CTCTGAAAAT CAAACCTAGG AACATAACAA GGAGAAAAAC AGACCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12665
    Clone ID Related Accession (Same CDS sequence) XM_005625210.3 , XM_005625210.2
    Accession Version XM_005625210.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGAGTG AAAGCACCAG CGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTATATTCC
    TCTGGCCAGA GCAGCAGGGA GCTGGCTCAC TGTGGAGGAG AGTCACCAGC TATGAAAGAT
    GGACATCCCA GAGACCCCTC ATCGGTCAGC AGCACACCTG GGAAGGAGAG CAGAGACAGC
    GACAGGGCCT CAAGGTCACC AGATGGTTCA GAGTTGGCTC GGTTGGAGGA GGAAGTGGAT
    GAGCTGTCCC TCATTGACCA CAATGAAATT ATGGCAAGGC TGACACTCAA GCAGGAGGGT
    GATGATGGGC CAGACGTCCG TGGAGGGTCT GGAGATATCC TACTGGTGCA CGCTACTGAG
    ACAGACAGGA AAGATTTGGT GTTGTACTGT GAGGCCTTCT TGACCACCTA CAGGACATTC
    ATCACCCCAG AGGAGCTCAT CAAGAAGCTG CAGTACAGGT ACGAGAAGTT CTCTCCCTTC
    GCTGACACCT TCAAGAAGCG CGTCAGCAAG AACACTTTCT TCGTGCTGGT GCGAGTGGTG
    GATGAGCTCT GCCTAGTGGA GTTGACAGAA GAGATCTTGA AGCTGCTCAT GGAACTGGTC
    TTTCGCTTGG TGTGCAACGG GGAGCTCAGC CTGGCCCGCG TGCTCCGGAA GAACATCCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCAACT CCGACCAGCC CCTGGCAGCC
    AGGGGGGTAG CTGCCAGGCC AGGGACTTTG CATGACTTTC ACAGCCATGA AATAGCCGAG
    CAGCTGACAC TGCTGGATGC TGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT
    TGGGCAAAAG AACAGAATGA GGAGAAAAGC CCCAACTTGA CCCAGTTCAC GGAGCACTTC
    AACAACATGT CGTACTGGGT CCGGTCAATA ATCATGTTAC AAGAAAAAGC CCAGGACAGG
    GAGCGACTGC TACTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTT
    AACTCCTACC TGGCCATCCT CTCGGCACTG GACTCAGCTC CCATCCGCAG GCTAGAGTGG
    CAGAAGCAGA CCTCGGAGGG CCTGGCCGAG TACTGTACAC TGATCGACAG CTCGTCCTCC
    TTCCGCGCCT ACCGGGCCGC CCTGTCCGAG GTGGAGCCGC CCTGCATCCC ATACCTGGGG
    CTTATCCTGC AGGACCTCAC CTTTGTTCAC CTGGGAAACC CAGACTATAT TGACGGGAAG
    GTGAATTTTT CCAAACGGTG GCAGCAGTTC AACATTCTGG ACAGCATGCG GTGCTTCCAA
    CAAGCGCACT ATGATATCCG AAGAAACGAT GACATCATAA ACTTCTTCAA TGACTTCAGT
    GACCACCTGG CGGAGGAGGC TCTGTGGGAA CTCTCTCTGA AAATCAAACC TAGGAACATA
    ACAAGGAGAA AAACAGACCG AGAGGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625267.1
    CDS354..4169
    Translation

    Target ORF information:

    RefSeq Version XM_005625210.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625210.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGAGTG AAAGCACCAG CGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTATATTCC
    TCTGGCCAGA GCAGCAGGGA GCTGGCTCAC TGTGGAGGAG AGTCACCAGC TATGAAAGAT
    GGACATCCCA GAGACCCCTC ATCGGTCAGC AGCACACCTG GGAAGGAGAG CAGAGACAGC
    GACAGGGCCT CAAGGTCACC AGATGGTTCA GAGTTGGCTC GGTTGGAGGA GGAAGTGGAT
    GAGCTGTCCC TCATTGACCA CAATGAAATT ATGGCAAGGC TGACACTCAA GCAGGAGGGT
    GATGATGGGC CAGACGTCCG TGGAGGGTCT GGAGATATCC TACTGGTGCA CGCTACTGAG
    ACAGACAGGA AAGATTTGGT GTTGTACTGT GAGGCCTTCT TGACCACCTA CAGGACATTC
    ATCACCCCAG AGGAGCTCAT CAAGAAGCTG CAGTACAGGT ACGAGAAGTT CTCTCCCTTC
    GCTGACACCT TCAAGAAGCG CGTCAGCAAG AACACTTTCT TCGTGCTGGT GCGAGTGGTG
    GATGAGCTCT GCCTAGTGGA GTTGACAGAA GAGATCTTGA AGCTGCTCAT GGAACTGGTC
    TTTCGCTTGG TGTGCAACGG GGAGCTCAGC CTGGCCCGCG TGCTCCGGAA GAACATCCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCAACT CCGACCAGCC CCTGGCAGCC
    AGGGGGGTAG CTGCCAGGCC AGGGACTTTG CATGACTTTC ACAGCCATGA AATAGCCGAG
    CAGCTGACAC TGCTGGATGC TGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT
    TGGGCAAAAG AACAGAATGA GGAGAAAAGC CCCAACTTGA CCCAGTTCAC GGAGCACTTC
    AACAACATGT CGTACTGGGT CCGGTCAATA ATCATGTTAC AAGAAAAAGC CCAGGACAGG
    GAGCGACTGC TACTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTT
    AACTCCTACC TGGCCATCCT CTCGGCACTG GACTCAGCTC CCATCCGCAG GCTAGAGTGG
    CAGAAGCAGA CCTCGGAGGG CCTGGCCGAG TACTGTACAC TGATCGACAG CTCGTCCTCC
    TTCCGCGCCT ACCGGGCCGC CCTGTCCGAG GTGGAGCCGC CCTGCATCCC ATACCTGGGG
    CTTATCCTGC AGGACCTCAC CTTTGTTCAC CTGGGAAACC CAGACTATAT TGACGGGAAG
    GTGAATTTTT CCAAACGGTG GCAGCAGTTC AACATTCTGG ACAGCATGCG GTGCTTCCAA
    CAAGCGCACT ATGATATCCG AAGAAACGAT GACATCATAA ACTTCTTCAA TGACTTCAGT
    GACCACCTGG CGGAGGAGGC TCTGTGGGAA CTCTCTCTGA AAATCAAACC TAGGAACATA
    ACAAGGAGAA AAACAGACCG AGAGGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12665
    Clone ID Related Accession (Same CDS sequence) XM_005625210.3 , XM_005625210.2
    Accession Version XM_005625210.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGAGTG AAAGCACCAG CGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTATATTCC
    TCTGGCCAGA GCAGCAGGGA GCTGGCTCAC TGTGGAGGAG AGTCACCAGC TATGAAAGAT
    GGACATCCCA GAGACCCCTC ATCGGTCAGC AGCACACCTG GGAAGGAGAG CAGAGACAGC
    GACAGGGCCT CAAGGTCACC AGATGGTTCA GAGTTGGCTC GGTTGGAGGA GGAAGTGGAT
    GAGCTGTCCC TCATTGACCA CAATGAAATT ATGGCAAGGC TGACACTCAA GCAGGAGGGT
    GATGATGGGC CAGACGTCCG TGGAGGGTCT GGAGATATCC TACTGGTGCA CGCTACTGAG
    ACAGACAGGA AAGATTTGGT GTTGTACTGT GAGGCCTTCT TGACCACCTA CAGGACATTC
    ATCACCCCAG AGGAGCTCAT CAAGAAGCTG CAGTACAGGT ACGAGAAGTT CTCTCCCTTC
    GCTGACACCT TCAAGAAGCG CGTCAGCAAG AACACTTTCT TCGTGCTGGT GCGAGTGGTG
    GATGAGCTCT GCCTAGTGGA GTTGACAGAA GAGATCTTGA AGCTGCTCAT GGAACTGGTC
    TTTCGCTTGG TGTGCAACGG GGAGCTCAGC CTGGCCCGCG TGCTCCGGAA GAACATCCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCAACT CCGACCAGCC CCTGGCAGCC
    AGGGGGGTAG CTGCCAGGCC AGGGACTTTG CATGACTTTC ACAGCCATGA AATAGCCGAG
    CAGCTGACAC TGCTGGATGC TGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT
    TGGGCAAAAG AACAGAATGA GGAGAAAAGC CCCAACTTGA CCCAGTTCAC GGAGCACTTC
    AACAACATGT CGTACTGGGT CCGGTCAATA ATCATGTTAC AAGAAAAAGC CCAGGACAGG
    GAGCGACTGC TACTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTT
    AACTCCTACC TGGCCATCCT CTCGGCACTG GACTCAGCTC CCATCCGCAG GCTAGAGTGG
    CAGAAGCAGA CCTCGGAGGG CCTGGCCGAG TACTGTACAC TGATCGACAG CTCGTCCTCC
    TTCCGCGCCT ACCGGGCCGC CCTGTCCGAG GTGGAGCCGC CCTGCATCCC ATACCTGGGG
    CTTATCCTGC AGGACCTCAC CTTTGTTCAC CTGGGAAACC CAGACTATAT TGACGGGAAG
    GTGAATTTTT CCAAACGGTG GCAGCAGTTC AACATTCTGG ACAGCATGCG GTGCTTCCAA
    CAAGCGCACT ATGATATCCG AAGAAACGAT GACATCATAA ACTTCTTCAA TGACTTCAGT
    GACCACCTGG CGGAGGAGGC TCTGTGGGAA CTCTCTCTGA AAATCAAACC TAGGAACATA
    ACAAGGAGAA AAACAGACCG AGAGGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625267.1
    CDS356..4171
    Translation

    Target ORF information:

    RefSeq Version XM_005625210.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625210.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGAGTG AAAGCACCAG CGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTATATTCC
    TCTGGCCAGA GCAGCAGGGA GCTGGCTCAC TGTGGAGGAG AGTCACCAGC TATGAAAGAT
    GGACATCCCA GAGACCCCTC ATCGGTCAGC AGCACACCTG GGAAGGAGAG CAGAGACAGC
    GACAGGGCCT CAAGGTCACC AGATGGTTCA GAGTTGGCTC GGTTGGAGGA GGAAGTGGAT
    GAGCTGTCCC TCATTGACCA CAATGAAATT ATGGCAAGGC TGACACTCAA GCAGGAGGGT
    GATGATGGGC CAGACGTCCG TGGAGGGTCT GGAGATATCC TACTGGTGCA CGCTACTGAG
    ACAGACAGGA AAGATTTGGT GTTGTACTGT GAGGCCTTCT TGACCACCTA CAGGACATTC
    ATCACCCCAG AGGAGCTCAT CAAGAAGCTG CAGTACAGGT ACGAGAAGTT CTCTCCCTTC
    GCTGACACCT TCAAGAAGCG CGTCAGCAAG AACACTTTCT TCGTGCTGGT GCGAGTGGTG
    GATGAGCTCT GCCTAGTGGA GTTGACAGAA GAGATCTTGA AGCTGCTCAT GGAACTGGTC
    TTTCGCTTGG TGTGCAACGG GGAGCTCAGC CTGGCCCGCG TGCTCCGGAA GAACATCCTG
    GACAAGGTGG ACCAGAAGAA GCTGCTCAGG TGTGCCAACT CCGACCAGCC CCTGGCAGCC
    AGGGGGGTAG CTGCCAGGCC AGGGACTTTG CATGACTTTC ACAGCCATGA AATAGCCGAG
    CAGCTGACAC TGCTGGATGC TGAGCTCTTT TATAAAATAG AGATTCCTGA GGTTTTGCTT
    TGGGCAAAAG AACAGAATGA GGAGAAAAGC CCCAACTTGA CCCAGTTCAC GGAGCACTTC
    AACAACATGT CGTACTGGGT CCGGTCAATA ATCATGTTAC AAGAAAAAGC CCAGGACAGG
    GAGCGACTGC TACTGAAGTT CATCAAGATC ATGAAGCACT TACGGAAGCT GAACAACTTT
    AACTCCTACC TGGCCATCCT CTCGGCACTG GACTCAGCTC CCATCCGCAG GCTAGAGTGG
    CAGAAGCAGA CCTCGGAGGG CCTGGCCGAG TACTGTACAC TGATCGACAG CTCGTCCTCC
    TTCCGCGCCT ACCGGGCCGC CCTGTCCGAG GTGGAGCCGC CCTGCATCCC ATACCTGGGG
    CTTATCCTGC AGGACCTCAC CTTTGTTCAC CTGGGAAACC CAGACTATAT TGACGGGAAG
    GTGAATTTTT CCAAACGGTG GCAGCAGTTC AACATTCTGG ACAGCATGCG GTGCTTCCAA
    CAAGCGCACT ATGATATCCG AAGAAACGAT GACATCATAA ACTTCTTCAA TGACTTCAGT
    GACCACCTGG CGGAGGAGGC TCTGTGGGAA CTCTCTCTGA AAATCAAACC TAGGAACATA
    ACAAGGAGAA AAACAGACCG AGAGGAGAAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12666
    Clone ID Related Accession (Same CDS sequence) XM_005625211.2 , XM_005625211.3
    Accession Version XM_005625211.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGAGTGAAA GCACCAGCGA GGAGGCTGGT GAGGGTGAAT ACGTCAATCT ATATTCCTCT
    GGCCAGAGCA GCAGGGAGCT GGCTCACTGT GGAGGAGAGT CACCAGCTAT GAAAGATGGA
    CATCCCAGAG ACCCCTCATC GGTCAGCAGC ACACCTGGGA AGGAGAGCAG AGACAGCGAC
    AGGGCCTCAA GGTCACCAGA TGGTTCAGAG TTGGCTCGGT TGGAGGAGGA AGTGGATGAG
    CTGTCCCTCA TTGACCACAA TGAAATTATG GCAAGGCTGA CACTCAAGCA GGAGGGTGAT
    GATGGGCCAG ACGTCCGTGG AGGGTCTGGA GATATCCTAC TGGTGCACGC TACTGAGACA
    GACAGGAAAG ATTTGGTGTT GTACTGTGAG GCCTTCTTGA CCACCTACAG GACATTCATC
    ACCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGGTACG AGAAGTTCTC TCCCTTCGCT
    GACACCTTCA AGAAGCGCGT CAGCAAGAAC ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT
    GAGCTCTGCC TAGTGGAGTT GACAGAAGAG ATCTTGAAGC TGCTCATGGA ACTGGTCTTT
    CGCTTGGTGT GCAACGGGGA GCTCAGCCTG GCCCGCGTGC TCCGGAAGAA CATCCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCAACTCCG ACCAGCCCCT GGCAGCCAGG
    GGGGTAGCTG CCAGGCCAGG GACTTTGCAT GACTTTCACA GCCATGAAAT AGCCGAGCAG
    CTGACACTGC TGGATGCTGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG
    GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC
    AACATGTCGT ACTGGGTCCG GTCAATAATC ATGTTACAAG AAAAAGCCCA GGACAGGGAG
    CGACTGCTAC TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTTAAC
    TCCTACCTGG CCATCCTCTC GGCACTGGAC TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG
    AAGCAGACCT CGGAGGGCCT GGCCGAGTAC TGTACACTGA TCGACAGCTC GTCCTCCTTC
    CGCGCCTACC GGGCCGCCCT GTCCGAGGTG GAGCCGCCCT GCATCCCATA CCTGGGGCTT
    ATCCTGCAGG ACCTCACCTT TGTTCACCTG GGAAACCCAG ACTATATTGA CGGGAAGGTG
    AATTTTTCCA AACGGTGGCA GCAGTTCAAC ATTCTGGACA GCATGCGGTG CTTCCAACAA
    GCGCACTATG ATATCCGAAG AAACGATGAC ATCATAAACT TCTTCAATGA CTTCAGTGAC
    CACCTGGCGG AGGAGGCTCT GTGGGAACTC TCTCTGAAAA TCAAACCTAG GAACATAACA
    AGGAGAAAAA CAGACCGAGA GGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625268.1
    CDS356..4168
    Translation

    Target ORF information:

    RefSeq Version XM_005625211.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625211.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGAGTGAAA GCACCAGCGA GGAGGCTGGT GAGGGTGAAT ACGTCAATCT ATATTCCTCT
    GGCCAGAGCA GCAGGGAGCT GGCTCACTGT GGAGGAGAGT CACCAGCTAT GAAAGATGGA
    CATCCCAGAG ACCCCTCATC GGTCAGCAGC ACACCTGGGA AGGAGAGCAG AGACAGCGAC
    AGGGCCTCAA GGTCACCAGA TGGTTCAGAG TTGGCTCGGT TGGAGGAGGA AGTGGATGAG
    CTGTCCCTCA TTGACCACAA TGAAATTATG GCAAGGCTGA CACTCAAGCA GGAGGGTGAT
    GATGGGCCAG ACGTCCGTGG AGGGTCTGGA GATATCCTAC TGGTGCACGC TACTGAGACA
    GACAGGAAAG ATTTGGTGTT GTACTGTGAG GCCTTCTTGA CCACCTACAG GACATTCATC
    ACCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGGTACG AGAAGTTCTC TCCCTTCGCT
    GACACCTTCA AGAAGCGCGT CAGCAAGAAC ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT
    GAGCTCTGCC TAGTGGAGTT GACAGAAGAG ATCTTGAAGC TGCTCATGGA ACTGGTCTTT
    CGCTTGGTGT GCAACGGGGA GCTCAGCCTG GCCCGCGTGC TCCGGAAGAA CATCCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCAACTCCG ACCAGCCCCT GGCAGCCAGG
    GGGGTAGCTG CCAGGCCAGG GACTTTGCAT GACTTTCACA GCCATGAAAT AGCCGAGCAG
    CTGACACTGC TGGATGCTGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG
    GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC
    AACATGTCGT ACTGGGTCCG GTCAATAATC ATGTTACAAG AAAAAGCCCA GGACAGGGAG
    CGACTGCTAC TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTTAAC
    TCCTACCTGG CCATCCTCTC GGCACTGGAC TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG
    AAGCAGACCT CGGAGGGCCT GGCCGAGTAC TGTACACTGA TCGACAGCTC GTCCTCCTTC
    CGCGCCTACC GGGCCGCCCT GTCCGAGGTG GAGCCGCCCT GCATCCCATA CCTGGGGCTT
    ATCCTGCAGG ACCTCACCTT TGTTCACCTG GGAAACCCAG ACTATATTGA CGGGAAGGTG
    AATTTTTCCA AACGGTGGCA GCAGTTCAAC ATTCTGGACA GCATGCGGTG CTTCCAACAA
    GCGCACTATG ATATCCGAAG AAACGATGAC ATCATAAACT TCTTCAATGA CTTCAGTGAC
    CACCTGGCGG AGGAGGCTCT GTGGGAACTC TCTCTGAAAA TCAAACCTAG GAACATAACA
    AGGAGAAAAA CAGACCGAGA GGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12666
    Clone ID Related Accession (Same CDS sequence) XM_005625211.2 , XM_005625211.3
    Accession Version XM_005625211.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGAGTGAAA GCACCAGCGA GGAGGCTGGT GAGGGTGAAT ACGTCAATCT ATATTCCTCT
    GGCCAGAGCA GCAGGGAGCT GGCTCACTGT GGAGGAGAGT CACCAGCTAT GAAAGATGGA
    CATCCCAGAG ACCCCTCATC GGTCAGCAGC ACACCTGGGA AGGAGAGCAG AGACAGCGAC
    AGGGCCTCAA GGTCACCAGA TGGTTCAGAG TTGGCTCGGT TGGAGGAGGA AGTGGATGAG
    CTGTCCCTCA TTGACCACAA TGAAATTATG GCAAGGCTGA CACTCAAGCA GGAGGGTGAT
    GATGGGCCAG ACGTCCGTGG AGGGTCTGGA GATATCCTAC TGGTGCACGC TACTGAGACA
    GACAGGAAAG ATTTGGTGTT GTACTGTGAG GCCTTCTTGA CCACCTACAG GACATTCATC
    ACCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGGTACG AGAAGTTCTC TCCCTTCGCT
    GACACCTTCA AGAAGCGCGT CAGCAAGAAC ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT
    GAGCTCTGCC TAGTGGAGTT GACAGAAGAG ATCTTGAAGC TGCTCATGGA ACTGGTCTTT
    CGCTTGGTGT GCAACGGGGA GCTCAGCCTG GCCCGCGTGC TCCGGAAGAA CATCCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCAACTCCG ACCAGCCCCT GGCAGCCAGG
    GGGGTAGCTG CCAGGCCAGG GACTTTGCAT GACTTTCACA GCCATGAAAT AGCCGAGCAG
    CTGACACTGC TGGATGCTGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG
    GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC
    AACATGTCGT ACTGGGTCCG GTCAATAATC ATGTTACAAG AAAAAGCCCA GGACAGGGAG
    CGACTGCTAC TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTTAAC
    TCCTACCTGG CCATCCTCTC GGCACTGGAC TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG
    AAGCAGACCT CGGAGGGCCT GGCCGAGTAC TGTACACTGA TCGACAGCTC GTCCTCCTTC
    CGCGCCTACC GGGCCGCCCT GTCCGAGGTG GAGCCGCCCT GCATCCCATA CCTGGGGCTT
    ATCCTGCAGG ACCTCACCTT TGTTCACCTG GGAAACCCAG ACTATATTGA CGGGAAGGTG
    AATTTTTCCA AACGGTGGCA GCAGTTCAAC ATTCTGGACA GCATGCGGTG CTTCCAACAA
    GCGCACTATG ATATCCGAAG AAACGATGAC ATCATAAACT TCTTCAATGA CTTCAGTGAC
    CACCTGGCGG AGGAGGCTCT GTGGGAACTC TCTCTGAAAA TCAAACCTAG GAACATAACA
    AGGAGAAAAA CAGACCGAGA GGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625268.1
    CDS354..4166
    Translation

    Target ORF information:

    RefSeq Version XM_005625211.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625211.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGAGTGAAA GCACCAGCGA GGAGGCTGGT GAGGGTGAAT ACGTCAATCT ATATTCCTCT
    GGCCAGAGCA GCAGGGAGCT GGCTCACTGT GGAGGAGAGT CACCAGCTAT GAAAGATGGA
    CATCCCAGAG ACCCCTCATC GGTCAGCAGC ACACCTGGGA AGGAGAGCAG AGACAGCGAC
    AGGGCCTCAA GGTCACCAGA TGGTTCAGAG TTGGCTCGGT TGGAGGAGGA AGTGGATGAG
    CTGTCCCTCA TTGACCACAA TGAAATTATG GCAAGGCTGA CACTCAAGCA GGAGGGTGAT
    GATGGGCCAG ACGTCCGTGG AGGGTCTGGA GATATCCTAC TGGTGCACGC TACTGAGACA
    GACAGGAAAG ATTTGGTGTT GTACTGTGAG GCCTTCTTGA CCACCTACAG GACATTCATC
    ACCCCAGAGG AGCTCATCAA GAAGCTGCAG TACAGGTACG AGAAGTTCTC TCCCTTCGCT
    GACACCTTCA AGAAGCGCGT CAGCAAGAAC ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT
    GAGCTCTGCC TAGTGGAGTT GACAGAAGAG ATCTTGAAGC TGCTCATGGA ACTGGTCTTT
    CGCTTGGTGT GCAACGGGGA GCTCAGCCTG GCCCGCGTGC TCCGGAAGAA CATCCTGGAC
    AAGGTGGACC AGAAGAAGCT GCTCAGGTGT GCCAACTCCG ACCAGCCCCT GGCAGCCAGG
    GGGGTAGCTG CCAGGCCAGG GACTTTGCAT GACTTTCACA GCCATGAAAT AGCCGAGCAG
    CTGACACTGC TGGATGCTGA GCTCTTTTAT AAAATAGAGA TTCCTGAGGT TTTGCTTTGG
    GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACTTGACCC AGTTCACGGA GCACTTCAAC
    AACATGTCGT ACTGGGTCCG GTCAATAATC ATGTTACAAG AAAAAGCCCA GGACAGGGAG
    CGACTGCTAC TGAAGTTCAT CAAGATCATG AAGCACTTAC GGAAGCTGAA CAACTTTAAC
    TCCTACCTGG CCATCCTCTC GGCACTGGAC TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG
    AAGCAGACCT CGGAGGGCCT GGCCGAGTAC TGTACACTGA TCGACAGCTC GTCCTCCTTC
    CGCGCCTACC GGGCCGCCCT GTCCGAGGTG GAGCCGCCCT GCATCCCATA CCTGGGGCTT
    ATCCTGCAGG ACCTCACCTT TGTTCACCTG GGAAACCCAG ACTATATTGA CGGGAAGGTG
    AATTTTTCCA AACGGTGGCA GCAGTTCAAC ATTCTGGACA GCATGCGGTG CTTCCAACAA
    GCGCACTATG ATATCCGAAG AAACGATGAC ATCATAAACT TCTTCAATGA CTTCAGTGAC
    CACCTGGCGG AGGAGGCTCT GTGGGAACTC TCTCTGAAAA TCAAACCTAG GAACATAACA
    AGGAGAAAAA CAGACCGAGA GGAGAAGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12667
    Clone ID Related Accession (Same CDS sequence) XM_005625212.3 , XM_005625212.2
    Accession Version XM_005625212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGATT CTCAGCGTTC TCACCTCTCT TCCTTCACCA TGAAGCTGAA GGACAAATTC 
    CACTCACCCA AAATCAAGAG AACGCCATCA AAGAAGGGAA AACCAGCTGA AGTTTCTGTG
    AAAAATCCGG AGAAGCCTGT GAGCAAAGAG GCAACAGACA GATTTCTACC AGAGGGCTAC
    CCTCTCCCCT TGGATCTGGA GCAGCAGGCA GTAGAATTTA TGTCCACCAG TGCTGTGGCT
    TCCAGGTCTC AGAGGCAGAA GAACCTGAGC TGGCTGGAAG AGAAGGAGAA GGAAGTTGTT
    AGTGCCTTGC GCTACTTTAA GACTATCGTG GACAAGATGG CTATTGATAA GAAGGTACTG
    GAGATGCTGC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAGC
    GATCCTCGAA TTCAACACAG CTCAGCCCTC TCCTCCTGCT ATAGCCGAGT GTACCAAAGT
    CTTGCCAACC TCATCCGTTG GTCTGACCAA GTGATGCTGG AAGGTGTGAA CTCAGAAGAT
    AAAGAGACGG TGACAACGGT GAAGGGGGTC ATCAAGGCGG TACTGGATGG AGTGAAGGAG
    CTGGTCAGAC TTACCATCGA GAAACAGGGG CATCCATCTC CAACCAGCCC AGTGAAACCC
    AGTTCCCCAG CCTGCAAACC GGACGGCCAG TCTGAGCTCC CCCTGACAGA TCGCGAGGTG
    GAGATTCTCA ACAAGACAAC TGGCATGTCC CAGTCGGCCG AGCTACTCCC AGACTCAACA
    GATGAGGAGG TTGCGCCCCC TAAGCCCCCC TTACCTGGCA TTCGGGTGGT TGATAACAGT
    CCTCCCCCAG CATTGCCACC GAAGAAAAGA CAGTCAGCTC CATCCCCGAC CCGAGTGGCT
    GTTGTTGCCC CCATGAGTCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATTAATAGG
    CAGGATTTTG ATGTGGACTG TTACACACAG AGGCGACTCT CAGGAGGCAG CCACTCATAC
    GGCGGAGAGT CGCCCCGTCT GTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAT
    GAGCAGCTGT CCTCTCTGGA CAGGGACAGC GGCCAGTGCT CCCGGAACAC AAGCTGTGAA
    ACACTAGATC ATTATGATCC TGACTACGAA TTCCTTCAGC AAGACCTCTC TAATGCAGAC
    CAGATACCTC AGCAAGTGGC CTGTAACCTT AGCCCATTGC CAGAGTCTTT GGGAGAGTCT
    GGGTCTCCAT TTCTTGGCCA TCCTTTCCAG TTGCCTCCAG CGCCTGGCAG CTGTCCCCAG
    CCAGAGGGGC CCTTGGCCCC AGGACAGCAG ATAGACATGC CACCTGCTCT CCCTGAGAAG
    AAGCGCAGGA GTGCGGCCTC CCAGACCACG GACAGCTCTG GCTGCAGGAT GTCCTACGAG
    CGGCACCCCT CACAATATGA CAATATCTCG GAAGATGACC TGCAGAACCC AGCCTCGGTC
    CCGTCCATCC CCTTCACGCC CTTTGCTGCT ATTCTCCCCT TTCAGCAAGG AGGGTCCTCA
    GCCTCTGTCG AATTTGTGGG TGATTTCACT GTTTCTGAAT CAACAACGGG TGACCCAGAA
    AAACCACCTC CTCTACCAGA GAAGAAAAAC AAACACATGC TGGCCTACAT GCAGCTGCTG
    GAGGACTACT CGGAGCCACA GCCGTCCATG TTCTACCAGA CGCCACAGAA CGAGCACATC
    TACCAGCAGA AGAACAAGCT CCTCATGGAG GTGTACGGCT TCAACGACTC ATTCAGCACC
    GAAGCCCCGC AGGAGCTGGC ACCGCCCCCC GCCCTGCCTC CCAAGCAGCG GCAGCTGCAG
    GCCTCCTATG CTGCTTCTTC CTTCTCCTCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG
    GCCTTCACCC CCGAGGACGG CAGTGCCACT CAGGGCATCA GTGTGTCCGT CTCTAACTCC
    TTTCTCAGCC GGCATGGCAA CTTGCCTGTG CCCTCGTATA AATCTGTGTT TAGGTCCTAC
    TCCCAGGATT TCGTGCCTCA CAACCACGCT TCCGTTCAGC CTTTCCTTCC GCCTACCTCC
    TCTGCCTCTC CACACTTCCC AACTGTCCAC CAGTCTCAGA GCTCTGACTT AACGGTGCCA
    ACCGTAGCCA GTCTGCCTCC CAGCACCGTG GACGGGCCTC TCTCGTCTTC TCAGGAGAGC
    AGCTTTCACG GGAACACTGT CTGCCTTCCT TCCGAAACCT CTTTCACTGA CTCGCCCCAG
    ACGCCTTTGA GTGAAAGCAC CAGCGAGGAG GCTGGTGAGG GTGAATACGT CAATCTATAT
    TCCTCTGGCC AGAGCAGCAG GGAGCTGGCT CACTGTGGAG GAGAGTCACC AGCTATGAAA
    GATGGACATC CCAGAGACCC CTCATCGGTC AGCAGCACAC CTGGGAAGGA GAGCAGAGAC
    AGCGACAGGG CCTCAAGGTC ACCAGATGGT TCAGAGTTGG CTCGGTTGGA GGAGGAAGTG
    GATGAGCTGT CCCTCATTGA CCACAATGAA ATTATGGCAA GGCTGACACT CAAGCAGGAG
    GGTGATGATG GGCCAGACGT CCGTGGAGGG TCTGGAGATA TCCTACTGGT GCACGCTACT
    GAGACAGACA GGAAAGATTT GGTGTTGTAC TGTGAGGCCT TCTTGACCAC CTACAGGACA
    TTCATCACCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GGTACGAGAA GTTCTCTCCC
    TTCGCTGACA CCTTCAAGAA GCGCGTCAGC AAGAACACTT TCTTCGTGCT GGTGCGAGTG
    GTGGATGAGC TCTGCCTAGT GGAGTTGACA GAAGAGATCT TGAAGCTGCT CATGGAACTG
    GTCTTTCGCT TGGTGTGCAA CGGGGAGCTC AGCCTGGCCC GCGTGCTCCG GAAGAACATC
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCA ACTCCGACCA GCCCCTGGCA
    GCCAGGGGGG TAGCTGCCAG GCCAGGGACT TTGCATGACT TTCACAGCCA TGAAATAGCC
    GAGCAGCTGA CACTGCTGGA TGCTGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG
    CTTTGGGCAA AAGAACAGAA TGAGGAGAAA AGCCCCAACT TGACCCAGTT CACGGAGCAC
    TTCAACAACA TGTCGTACTG GGTCCGGTCA ATAATCATGT TACAAGAAAA AGCCCAGGAC
    AGGGAGCGAC TGCTACTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC
    TTTAACTCCT ACCTGGCCAT CCTCTCGGCA CTGGACTCAG CTCCCATCCG CAGGCTAGAG
    TGGCAGAAGC AGACCTCGGA GGGCCTGGCC GAGTACTGTA CACTGATCGA CAGCTCGTCC
    TCCTTCCGCG CCTACCGGGC CGCCCTGTCC GAGGTGGAGC CGCCCTGCAT CCCATACCTG
    GGGCTTATCC TGCAGGACCT CACCTTTGTT CACCTGGGAA ACCCAGACTA TATTGACGGG
    AAGGTGAATT TTTCCAAACG GTGGCAGCAG TTCAACATTC TGGACAGCAT GCGGTGCTTC
    CAACAAGCGC ACTATGATAT CCGAAGAAAC GATGACATCA TAAACTTCTT CAATGACTTC
    AGTGACCACC TGGCGGAGGA GGCTCTGTGG GAACTCTCTC TGAAAATCAA ACCTAGGAAC
    ATAACAAGGA GAAAAACAGA CCGAGAGGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625269.1
    CDS18..3776
    Translation

    Target ORF information:

    RefSeq Version XM_005625212.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGATT CTCAGCGTTC TCACCTCTCT TCCTTCACCA TGAAGCTGAA GGACAAATTC 
    CACTCACCCA AAATCAAGAG AACGCCATCA AAGAAGGGAA AACCAGCTGA AGTTTCTGTG
    AAAAATCCGG AGAAGCCTGT GAGCAAAGAG GCAACAGACA GATTTCTACC AGAGGGCTAC
    CCTCTCCCCT TGGATCTGGA GCAGCAGGCA GTAGAATTTA TGTCCACCAG TGCTGTGGCT
    TCCAGGTCTC AGAGGCAGAA GAACCTGAGC TGGCTGGAAG AGAAGGAGAA GGAAGTTGTT
    AGTGCCTTGC GCTACTTTAA GACTATCGTG GACAAGATGG CTATTGATAA GAAGGTACTG
    GAGATGCTGC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAGC
    GATCCTCGAA TTCAACACAG CTCAGCCCTC TCCTCCTGCT ATAGCCGAGT GTACCAAAGT
    CTTGCCAACC TCATCCGTTG GTCTGACCAA GTGATGCTGG AAGGTGTGAA CTCAGAAGAT
    AAAGAGACGG TGACAACGGT GAAGGGGGTC ATCAAGGCGG TACTGGATGG AGTGAAGGAG
    CTGGTCAGAC TTACCATCGA GAAACAGGGG CATCCATCTC CAACCAGCCC AGTGAAACCC
    AGTTCCCCAG CCTGCAAACC GGACGGCCAG TCTGAGCTCC CCCTGACAGA TCGCGAGGTG
    GAGATTCTCA ACAAGACAAC TGGCATGTCC CAGTCGGCCG AGCTACTCCC AGACTCAACA
    GATGAGGAGG TTGCGCCCCC TAAGCCCCCC TTACCTGGCA TTCGGGTGGT TGATAACAGT
    CCTCCCCCAG CATTGCCACC GAAGAAAAGA CAGTCAGCTC CATCCCCGAC CCGAGTGGCT
    GTTGTTGCCC CCATGAGTCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATTAATAGG
    CAGGATTTTG ATGTGGACTG TTACACACAG AGGCGACTCT CAGGAGGCAG CCACTCATAC
    GGCGGAGAGT CGCCCCGTCT GTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAT
    GAGCAGCTGT CCTCTCTGGA CAGGGACAGC GGCCAGTGCT CCCGGAACAC AAGCTGTGAA
    ACACTAGATC ATTATGATCC TGACTACGAA TTCCTTCAGC AAGACCTCTC TAATGCAGAC
    CAGATACCTC AGCAAGTGGC CTGTAACCTT AGCCCATTGC CAGAGTCTTT GGGAGAGTCT
    GGGTCTCCAT TTCTTGGCCA TCCTTTCCAG TTGCCTCCAG CGCCTGGCAG CTGTCCCCAG
    CCAGAGGGGC CCTTGGCCCC AGGACAGCAG ATAGACATGC CACCTGCTCT CCCTGAGAAG
    AAGCGCAGGA GTGCGGCCTC CCAGACCACG GACAGCTCTG GCTGCAGGAT GTCCTACGAG
    CGGCACCCCT CACAATATGA CAATATCTCG GAAGATGACC TGCAGAACCC AGCCTCGGTC
    CCGTCCATCC CCTTCACGCC CTTTGCTGCT ATTCTCCCCT TTCAGCAAGG AGGGTCCTCA
    GCCTCTGTCG AATTTGTGGG TGATTTCACT GTTTCTGAAT CAACAACGGG TGACCCAGAA
    AAACCACCTC CTCTACCAGA GAAGAAAAAC AAACACATGC TGGCCTACAT GCAGCTGCTG
    GAGGACTACT CGGAGCCACA GCCGTCCATG TTCTACCAGA CGCCACAGAA CGAGCACATC
    TACCAGCAGA AGAACAAGCT CCTCATGGAG GTGTACGGCT TCAACGACTC ATTCAGCACC
    GAAGCCCCGC AGGAGCTGGC ACCGCCCCCC GCCCTGCCTC CCAAGCAGCG GCAGCTGCAG
    GCCTCCTATG CTGCTTCTTC CTTCTCCTCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG
    GCCTTCACCC CCGAGGACGG CAGTGCCACT CAGGGCATCA GTGTGTCCGT CTCTAACTCC
    TTTCTCAGCC GGCATGGCAA CTTGCCTGTG CCCTCGTATA AATCTGTGTT TAGGTCCTAC
    TCCCAGGATT TCGTGCCTCA CAACCACGCT TCCGTTCAGC CTTTCCTTCC GCCTACCTCC
    TCTGCCTCTC CACACTTCCC AACTGTCCAC CAGTCTCAGA GCTCTGACTT AACGGTGCCA
    ACCGTAGCCA GTCTGCCTCC CAGCACCGTG GACGGGCCTC TCTCGTCTTC TCAGGAGAGC
    AGCTTTCACG GGAACACTGT CTGCCTTCCT TCCGAAACCT CTTTCACTGA CTCGCCCCAG
    ACGCCTTTGA GTGAAAGCAC CAGCGAGGAG GCTGGTGAGG GTGAATACGT CAATCTATAT
    TCCTCTGGCC AGAGCAGCAG GGAGCTGGCT CACTGTGGAG GAGAGTCACC AGCTATGAAA
    GATGGACATC CCAGAGACCC CTCATCGGTC AGCAGCACAC CTGGGAAGGA GAGCAGAGAC
    AGCGACAGGG CCTCAAGGTC ACCAGATGGT TCAGAGTTGG CTCGGTTGGA GGAGGAAGTG
    GATGAGCTGT CCCTCATTGA CCACAATGAA ATTATGGCAA GGCTGACACT CAAGCAGGAG
    GGTGATGATG GGCCAGACGT CCGTGGAGGG TCTGGAGATA TCCTACTGGT GCACGCTACT
    GAGACAGACA GGAAAGATTT GGTGTTGTAC TGTGAGGCCT TCTTGACCAC CTACAGGACA
    TTCATCACCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GGTACGAGAA GTTCTCTCCC
    TTCGCTGACA CCTTCAAGAA GCGCGTCAGC AAGAACACTT TCTTCGTGCT GGTGCGAGTG
    GTGGATGAGC TCTGCCTAGT GGAGTTGACA GAAGAGATCT TGAAGCTGCT CATGGAACTG
    GTCTTTCGCT TGGTGTGCAA CGGGGAGCTC AGCCTGGCCC GCGTGCTCCG GAAGAACATC
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCA ACTCCGACCA GCCCCTGGCA
    GCCAGGGGGG TAGCTGCCAG GCCAGGGACT TTGCATGACT TTCACAGCCA TGAAATAGCC
    GAGCAGCTGA CACTGCTGGA TGCTGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG
    CTTTGGGCAA AAGAACAGAA TGAGGAGAAA AGCCCCAACT TGACCCAGTT CACGGAGCAC
    TTCAACAACA TGTCGTACTG GGTCCGGTCA ATAATCATGT TACAAGAAAA AGCCCAGGAC
    AGGGAGCGAC TGCTACTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC
    TTTAACTCCT ACCTGGCCAT CCTCTCGGCA CTGGACTCAG CTCCCATCCG CAGGCTAGAG
    TGGCAGAAGC AGACCTCGGA GGGCCTGGCC GAGTACTGTA CACTGATCGA CAGCTCGTCC
    TCCTTCCGCG CCTACCGGGC CGCCCTGTCC GAGGTGGAGC CGCCCTGCAT CCCATACCTG
    GGGCTTATCC TGCAGGACCT CACCTTTGTT CACCTGGGAA ACCCAGACTA TATTGACGGG
    AAGGTGAATT TTTCCAAACG GTGGCAGCAG TTCAACATTC TGGACAGCAT GCGGTGCTTC
    CAACAAGCGC ACTATGATAT CCGAAGAAAC GATGACATCA TAAACTTCTT CAATGACTTC
    AGTGACCACC TGGCGGAGGA GGCTCTGTGG GAACTCTCTC TGAAAATCAA ACCTAGGAAC
    ATAACAAGGA GAAAAACAGA CCGAGAGGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12667
    Clone ID Related Accession (Same CDS sequence) XM_005625212.3 , XM_005625212.2
    Accession Version XM_005625212.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGATT CTCAGCGTTC TCACCTCTCT TCCTTCACCA TGAAGCTGAA GGACAAATTC 
    CACTCACCCA AAATCAAGAG AACGCCATCA AAGAAGGGAA AACCAGCTGA AGTTTCTGTG
    AAAAATCCGG AGAAGCCTGT GAGCAAAGAG GCAACAGACA GATTTCTACC AGAGGGCTAC
    CCTCTCCCCT TGGATCTGGA GCAGCAGGCA GTAGAATTTA TGTCCACCAG TGCTGTGGCT
    TCCAGGTCTC AGAGGCAGAA GAACCTGAGC TGGCTGGAAG AGAAGGAGAA GGAAGTTGTT
    AGTGCCTTGC GCTACTTTAA GACTATCGTG GACAAGATGG CTATTGATAA GAAGGTACTG
    GAGATGCTGC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAGC
    GATCCTCGAA TTCAACACAG CTCAGCCCTC TCCTCCTGCT ATAGCCGAGT GTACCAAAGT
    CTTGCCAACC TCATCCGTTG GTCTGACCAA GTGATGCTGG AAGGTGTGAA CTCAGAAGAT
    AAAGAGACGG TGACAACGGT GAAGGGGGTC ATCAAGGCGG TACTGGATGG AGTGAAGGAG
    CTGGTCAGAC TTACCATCGA GAAACAGGGG CATCCATCTC CAACCAGCCC AGTGAAACCC
    AGTTCCCCAG CCTGCAAACC GGACGGCCAG TCTGAGCTCC CCCTGACAGA TCGCGAGGTG
    GAGATTCTCA ACAAGACAAC TGGCATGTCC CAGTCGGCCG AGCTACTCCC AGACTCAACA
    GATGAGGAGG TTGCGCCCCC TAAGCCCCCC TTACCTGGCA TTCGGGTGGT TGATAACAGT
    CCTCCCCCAG CATTGCCACC GAAGAAAAGA CAGTCAGCTC CATCCCCGAC CCGAGTGGCT
    GTTGTTGCCC CCATGAGTCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATTAATAGG
    CAGGATTTTG ATGTGGACTG TTACACACAG AGGCGACTCT CAGGAGGCAG CCACTCATAC
    GGCGGAGAGT CGCCCCGTCT GTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAT
    GAGCAGCTGT CCTCTCTGGA CAGGGACAGC GGCCAGTGCT CCCGGAACAC AAGCTGTGAA
    ACACTAGATC ATTATGATCC TGACTACGAA TTCCTTCAGC AAGACCTCTC TAATGCAGAC
    CAGATACCTC AGCAAGTGGC CTGTAACCTT AGCCCATTGC CAGAGTCTTT GGGAGAGTCT
    GGGTCTCCAT TTCTTGGCCA TCCTTTCCAG TTGCCTCCAG CGCCTGGCAG CTGTCCCCAG
    CCAGAGGGGC CCTTGGCCCC AGGACAGCAG ATAGACATGC CACCTGCTCT CCCTGAGAAG
    AAGCGCAGGA GTGCGGCCTC CCAGACCACG GACAGCTCTG GCTGCAGGAT GTCCTACGAG
    CGGCACCCCT CACAATATGA CAATATCTCG GAAGATGACC TGCAGAACCC AGCCTCGGTC
    CCGTCCATCC CCTTCACGCC CTTTGCTGCT ATTCTCCCCT TTCAGCAAGG AGGGTCCTCA
    GCCTCTGTCG AATTTGTGGG TGATTTCACT GTTTCTGAAT CAACAACGGG TGACCCAGAA
    AAACCACCTC CTCTACCAGA GAAGAAAAAC AAACACATGC TGGCCTACAT GCAGCTGCTG
    GAGGACTACT CGGAGCCACA GCCGTCCATG TTCTACCAGA CGCCACAGAA CGAGCACATC
    TACCAGCAGA AGAACAAGCT CCTCATGGAG GTGTACGGCT TCAACGACTC ATTCAGCACC
    GAAGCCCCGC AGGAGCTGGC ACCGCCCCCC GCCCTGCCTC CCAAGCAGCG GCAGCTGCAG
    GCCTCCTATG CTGCTTCTTC CTTCTCCTCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG
    GCCTTCACCC CCGAGGACGG CAGTGCCACT CAGGGCATCA GTGTGTCCGT CTCTAACTCC
    TTTCTCAGCC GGCATGGCAA CTTGCCTGTG CCCTCGTATA AATCTGTGTT TAGGTCCTAC
    TCCCAGGATT TCGTGCCTCA CAACCACGCT TCCGTTCAGC CTTTCCTTCC GCCTACCTCC
    TCTGCCTCTC CACACTTCCC AACTGTCCAC CAGTCTCAGA GCTCTGACTT AACGGTGCCA
    ACCGTAGCCA GTCTGCCTCC CAGCACCGTG GACGGGCCTC TCTCGTCTTC TCAGGAGAGC
    AGCTTTCACG GGAACACTGT CTGCCTTCCT TCCGAAACCT CTTTCACTGA CTCGCCCCAG
    ACGCCTTTGA GTGAAAGCAC CAGCGAGGAG GCTGGTGAGG GTGAATACGT CAATCTATAT
    TCCTCTGGCC AGAGCAGCAG GGAGCTGGCT CACTGTGGAG GAGAGTCACC AGCTATGAAA
    GATGGACATC CCAGAGACCC CTCATCGGTC AGCAGCACAC CTGGGAAGGA GAGCAGAGAC
    AGCGACAGGG CCTCAAGGTC ACCAGATGGT TCAGAGTTGG CTCGGTTGGA GGAGGAAGTG
    GATGAGCTGT CCCTCATTGA CCACAATGAA ATTATGGCAA GGCTGACACT CAAGCAGGAG
    GGTGATGATG GGCCAGACGT CCGTGGAGGG TCTGGAGATA TCCTACTGGT GCACGCTACT
    GAGACAGACA GGAAAGATTT GGTGTTGTAC TGTGAGGCCT TCTTGACCAC CTACAGGACA
    TTCATCACCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GGTACGAGAA GTTCTCTCCC
    TTCGCTGACA CCTTCAAGAA GCGCGTCAGC AAGAACACTT TCTTCGTGCT GGTGCGAGTG
    GTGGATGAGC TCTGCCTAGT GGAGTTGACA GAAGAGATCT TGAAGCTGCT CATGGAACTG
    GTCTTTCGCT TGGTGTGCAA CGGGGAGCTC AGCCTGGCCC GCGTGCTCCG GAAGAACATC
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCA ACTCCGACCA GCCCCTGGCA
    GCCAGGGGGG TAGCTGCCAG GCCAGGGACT TTGCATGACT TTCACAGCCA TGAAATAGCC
    GAGCAGCTGA CACTGCTGGA TGCTGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG
    CTTTGGGCAA AAGAACAGAA TGAGGAGAAA AGCCCCAACT TGACCCAGTT CACGGAGCAC
    TTCAACAACA TGTCGTACTG GGTCCGGTCA ATAATCATGT TACAAGAAAA AGCCCAGGAC
    AGGGAGCGAC TGCTACTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC
    TTTAACTCCT ACCTGGCCAT CCTCTCGGCA CTGGACTCAG CTCCCATCCG CAGGCTAGAG
    TGGCAGAAGC AGACCTCGGA GGGCCTGGCC GAGTACTGTA CACTGATCGA CAGCTCGTCC
    TCCTTCCGCG CCTACCGGGC CGCCCTGTCC GAGGTGGAGC CGCCCTGCAT CCCATACCTG
    GGGCTTATCC TGCAGGACCT CACCTTTGTT CACCTGGGAA ACCCAGACTA TATTGACGGG
    AAGGTGAATT TTTCCAAACG GTGGCAGCAG TTCAACATTC TGGACAGCAT GCGGTGCTTC
    CAACAAGCGC ACTATGATAT CCGAAGAAAC GATGACATCA TAAACTTCTT CAATGACTTC
    AGTGACCACC TGGCGGAGGA GGCTCTGTGG GAACTCTCTC TGAAAATCAA ACCTAGGAAC
    ATAACAAGGA GAAAAACAGA CCGAGAGGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625269.1
    CDS158..3916
    Translation

    Target ORF information:

    RefSeq Version XM_005625212.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGATT CTCAGCGTTC TCACCTCTCT TCCTTCACCA TGAAGCTGAA GGACAAATTC 
    CACTCACCCA AAATCAAGAG AACGCCATCA AAGAAGGGAA AACCAGCTGA AGTTTCTGTG
    AAAAATCCGG AGAAGCCTGT GAGCAAAGAG GCAACAGACA GATTTCTACC AGAGGGCTAC
    CCTCTCCCCT TGGATCTGGA GCAGCAGGCA GTAGAATTTA TGTCCACCAG TGCTGTGGCT
    TCCAGGTCTC AGAGGCAGAA GAACCTGAGC TGGCTGGAAG AGAAGGAGAA GGAAGTTGTT
    AGTGCCTTGC GCTACTTTAA GACTATCGTG GACAAGATGG CTATTGATAA GAAGGTACTG
    GAGATGCTGC CAGGGTCAGC CAGCAAGGTG CTGGAGGCCA TCTTACCCCT GGTGCAGAGC
    GATCCTCGAA TTCAACACAG CTCAGCCCTC TCCTCCTGCT ATAGCCGAGT GTACCAAAGT
    CTTGCCAACC TCATCCGTTG GTCTGACCAA GTGATGCTGG AAGGTGTGAA CTCAGAAGAT
    AAAGAGACGG TGACAACGGT GAAGGGGGTC ATCAAGGCGG TACTGGATGG AGTGAAGGAG
    CTGGTCAGAC TTACCATCGA GAAACAGGGG CATCCATCTC CAACCAGCCC AGTGAAACCC
    AGTTCCCCAG CCTGCAAACC GGACGGCCAG TCTGAGCTCC CCCTGACAGA TCGCGAGGTG
    GAGATTCTCA ACAAGACAAC TGGCATGTCC CAGTCGGCCG AGCTACTCCC AGACTCAACA
    GATGAGGAGG TTGCGCCCCC TAAGCCCCCC TTACCTGGCA TTCGGGTGGT TGATAACAGT
    CCTCCCCCAG CATTGCCACC GAAGAAAAGA CAGTCAGCTC CATCCCCGAC CCGAGTGGCT
    GTTGTTGCCC CCATGAGTCG AGCCACCAGT GGCTCCAGTT TGCCTGTTGG AATTAATAGG
    CAGGATTTTG ATGTGGACTG TTACACACAG AGGCGACTCT CAGGAGGCAG CCACTCATAC
    GGCGGAGAGT CGCCCCGTCT GTCCCCCTGC AGCAGCATAG GCAAGCTCAG CAAGTCAGAT
    GAGCAGCTGT CCTCTCTGGA CAGGGACAGC GGCCAGTGCT CCCGGAACAC AAGCTGTGAA
    ACACTAGATC ATTATGATCC TGACTACGAA TTCCTTCAGC AAGACCTCTC TAATGCAGAC
    CAGATACCTC AGCAAGTGGC CTGTAACCTT AGCCCATTGC CAGAGTCTTT GGGAGAGTCT
    GGGTCTCCAT TTCTTGGCCA TCCTTTCCAG TTGCCTCCAG CGCCTGGCAG CTGTCCCCAG
    CCAGAGGGGC CCTTGGCCCC AGGACAGCAG ATAGACATGC CACCTGCTCT CCCTGAGAAG
    AAGCGCAGGA GTGCGGCCTC CCAGACCACG GACAGCTCTG GCTGCAGGAT GTCCTACGAG
    CGGCACCCCT CACAATATGA CAATATCTCG GAAGATGACC TGCAGAACCC AGCCTCGGTC
    CCGTCCATCC CCTTCACGCC CTTTGCTGCT ATTCTCCCCT TTCAGCAAGG AGGGTCCTCA
    GCCTCTGTCG AATTTGTGGG TGATTTCACT GTTTCTGAAT CAACAACGGG TGACCCAGAA
    AAACCACCTC CTCTACCAGA GAAGAAAAAC AAACACATGC TGGCCTACAT GCAGCTGCTG
    GAGGACTACT CGGAGCCACA GCCGTCCATG TTCTACCAGA CGCCACAGAA CGAGCACATC
    TACCAGCAGA AGAACAAGCT CCTCATGGAG GTGTACGGCT TCAACGACTC ATTCAGCACC
    GAAGCCCCGC AGGAGCTGGC ACCGCCCCCC GCCCTGCCTC CCAAGCAGCG GCAGCTGCAG
    GCCTCCTATG CTGCTTCTTC CTTCTCCTCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG
    GCCTTCACCC CCGAGGACGG CAGTGCCACT CAGGGCATCA GTGTGTCCGT CTCTAACTCC
    TTTCTCAGCC GGCATGGCAA CTTGCCTGTG CCCTCGTATA AATCTGTGTT TAGGTCCTAC
    TCCCAGGATT TCGTGCCTCA CAACCACGCT TCCGTTCAGC CTTTCCTTCC GCCTACCTCC
    TCTGCCTCTC CACACTTCCC AACTGTCCAC CAGTCTCAGA GCTCTGACTT AACGGTGCCA
    ACCGTAGCCA GTCTGCCTCC CAGCACCGTG GACGGGCCTC TCTCGTCTTC TCAGGAGAGC
    AGCTTTCACG GGAACACTGT CTGCCTTCCT TCCGAAACCT CTTTCACTGA CTCGCCCCAG
    ACGCCTTTGA GTGAAAGCAC CAGCGAGGAG GCTGGTGAGG GTGAATACGT CAATCTATAT
    TCCTCTGGCC AGAGCAGCAG GGAGCTGGCT CACTGTGGAG GAGAGTCACC AGCTATGAAA
    GATGGACATC CCAGAGACCC CTCATCGGTC AGCAGCACAC CTGGGAAGGA GAGCAGAGAC
    AGCGACAGGG CCTCAAGGTC ACCAGATGGT TCAGAGTTGG CTCGGTTGGA GGAGGAAGTG
    GATGAGCTGT CCCTCATTGA CCACAATGAA ATTATGGCAA GGCTGACACT CAAGCAGGAG
    GGTGATGATG GGCCAGACGT CCGTGGAGGG TCTGGAGATA TCCTACTGGT GCACGCTACT
    GAGACAGACA GGAAAGATTT GGTGTTGTAC TGTGAGGCCT TCTTGACCAC CTACAGGACA
    TTCATCACCC CAGAGGAGCT CATCAAGAAG CTGCAGTACA GGTACGAGAA GTTCTCTCCC
    TTCGCTGACA CCTTCAAGAA GCGCGTCAGC AAGAACACTT TCTTCGTGCT GGTGCGAGTG
    GTGGATGAGC TCTGCCTAGT GGAGTTGACA GAAGAGATCT TGAAGCTGCT CATGGAACTG
    GTCTTTCGCT TGGTGTGCAA CGGGGAGCTC AGCCTGGCCC GCGTGCTCCG GAAGAACATC
    CTGGACAAGG TGGACCAGAA GAAGCTGCTC AGGTGTGCCA ACTCCGACCA GCCCCTGGCA
    GCCAGGGGGG TAGCTGCCAG GCCAGGGACT TTGCATGACT TTCACAGCCA TGAAATAGCC
    GAGCAGCTGA CACTGCTGGA TGCTGAGCTC TTTTATAAAA TAGAGATTCC TGAGGTTTTG
    CTTTGGGCAA AAGAACAGAA TGAGGAGAAA AGCCCCAACT TGACCCAGTT CACGGAGCAC
    TTCAACAACA TGTCGTACTG GGTCCGGTCA ATAATCATGT TACAAGAAAA AGCCCAGGAC
    AGGGAGCGAC TGCTACTGAA GTTCATCAAG ATCATGAAGC ACTTACGGAA GCTGAACAAC
    TTTAACTCCT ACCTGGCCAT CCTCTCGGCA CTGGACTCAG CTCCCATCCG CAGGCTAGAG
    TGGCAGAAGC AGACCTCGGA GGGCCTGGCC GAGTACTGTA CACTGATCGA CAGCTCGTCC
    TCCTTCCGCG CCTACCGGGC CGCCCTGTCC GAGGTGGAGC CGCCCTGCAT CCCATACCTG
    GGGCTTATCC TGCAGGACCT CACCTTTGTT CACCTGGGAA ACCCAGACTA TATTGACGGG
    AAGGTGAATT TTTCCAAACG GTGGCAGCAG TTCAACATTC TGGACAGCAT GCGGTGCTTC
    CAACAAGCGC ACTATGATAT CCGAAGAAAC GATGACATCA TAAACTTCTT CAATGACTTC
    AGTGACCACC TGGCGGAGGA GGCTCTGTGG GAACTCTCTC TGAAAATCAA ACCTAGGAAC
    ATAACAAGGA GAAAAACAGA CCGAGAGGAG AAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12668
    Clone ID Related Accession (Same CDS sequence) XM_005625213.2 , XM_005625213.3
    Accession Version XM_005625213.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGGAGT CACCAGCTAT GAAAGATGGA CATCCCAGAG ACCCCTCATC GGTCAGCAGC
    ACACCTGGGA AGGAGAGCAG AGACAGCGAC AGGGCCTCAA GGTCACCAGA TGGTTCAGAG
    TTGGCTCGGT TGGAGGAGGA AGTGGATGAG CTGTCCCTCA TTGACCACAA TGAAATTATG
    GCAAGGCTGA CACTCAAGCA GGAGGGTGAT GATGGGCCAG ACGTCCGTGG AGGGTCTGGA
    GATATCCTAC TGGTGCACGC TACTGAGACA GACAGGAAAG ATTTGGTGTT GTACTGTGAG
    GCCTTCTTGA CCACCTACAG GACATTCATC ACCCCAGAGG AGCTCATCAA GAAGCTGCAG
    TACAGGTACG AGAAGTTCTC TCCCTTCGCT GACACCTTCA AGAAGCGCGT CAGCAAGAAC
    ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT GAGCTCTGCC TAGTGGAGTT GACAGAAGAG
    ATCTTGAAGC TGCTCATGGA ACTGGTCTTT CGCTTGGTGT GCAACGGGGA GCTCAGCCTG
    GCCCGCGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT GCTCAGGTGT
    GCCAACTCCG ACCAGCCCCT GGCAGCCAGG GGGGTAGCTG CCAGGCCAGG GACTTTGCAT
    GACTTTCACA GCCATGAAAT AGCCGAGCAG CTGACACTGC TGGATGCTGA GCTCTTTTAT
    AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAAC AGAATGAGGA GAAAAGCCCC
    AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCGT ACTGGGTCCG GTCAATAATC
    ATGTTACAAG AAAAAGCCCA GGACAGGGAG CGACTGCTAC TGAAGTTCAT CAAGATCATG
    AAGCACTTAC GGAAGCTGAA CAACTTTAAC TCCTACCTGG CCATCCTCTC GGCACTGGAC
    TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG AAGCAGACCT CGGAGGGCCT GGCCGAGTAC
    TGTACACTGA TCGACAGCTC GTCCTCCTTC CGCGCCTACC GGGCCGCCCT GTCCGAGGTG
    GAGCCGCCCT GCATCCCATA CCTGGGGCTT ATCCTGCAGG ACCTCACCTT TGTTCACCTG
    GGAAACCCAG ACTATATTGA CGGGAAGGTG AATTTTTCCA AACGGTGGCA GCAGTTCAAC
    ATTCTGGACA GCATGCGGTG CTTCCAACAA GCGCACTATG ATATCCGAAG AAACGATGAC
    ATCATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCGG AGGAGGCTCT GTGGGAACTC
    TCTCTGAAAA TCAAACCTAG GAACATAACA AGGAGAAAAA CAGACCGAGA GGAGAAGACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625270.1
    CDS355..4077
    Translation

    Target ORF information:

    RefSeq Version XM_005625213.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625213.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGGAGT CACCAGCTAT GAAAGATGGA CATCCCAGAG ACCCCTCATC GGTCAGCAGC
    ACACCTGGGA AGGAGAGCAG AGACAGCGAC AGGGCCTCAA GGTCACCAGA TGGTTCAGAG
    TTGGCTCGGT TGGAGGAGGA AGTGGATGAG CTGTCCCTCA TTGACCACAA TGAAATTATG
    GCAAGGCTGA CACTCAAGCA GGAGGGTGAT GATGGGCCAG ACGTCCGTGG AGGGTCTGGA
    GATATCCTAC TGGTGCACGC TACTGAGACA GACAGGAAAG ATTTGGTGTT GTACTGTGAG
    GCCTTCTTGA CCACCTACAG GACATTCATC ACCCCAGAGG AGCTCATCAA GAAGCTGCAG
    TACAGGTACG AGAAGTTCTC TCCCTTCGCT GACACCTTCA AGAAGCGCGT CAGCAAGAAC
    ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT GAGCTCTGCC TAGTGGAGTT GACAGAAGAG
    ATCTTGAAGC TGCTCATGGA ACTGGTCTTT CGCTTGGTGT GCAACGGGGA GCTCAGCCTG
    GCCCGCGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT GCTCAGGTGT
    GCCAACTCCG ACCAGCCCCT GGCAGCCAGG GGGGTAGCTG CCAGGCCAGG GACTTTGCAT
    GACTTTCACA GCCATGAAAT AGCCGAGCAG CTGACACTGC TGGATGCTGA GCTCTTTTAT
    AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAAC AGAATGAGGA GAAAAGCCCC
    AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCGT ACTGGGTCCG GTCAATAATC
    ATGTTACAAG AAAAAGCCCA GGACAGGGAG CGACTGCTAC TGAAGTTCAT CAAGATCATG
    AAGCACTTAC GGAAGCTGAA CAACTTTAAC TCCTACCTGG CCATCCTCTC GGCACTGGAC
    TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG AAGCAGACCT CGGAGGGCCT GGCCGAGTAC
    TGTACACTGA TCGACAGCTC GTCCTCCTTC CGCGCCTACC GGGCCGCCCT GTCCGAGGTG
    GAGCCGCCCT GCATCCCATA CCTGGGGCTT ATCCTGCAGG ACCTCACCTT TGTTCACCTG
    GGAAACCCAG ACTATATTGA CGGGAAGGTG AATTTTTCCA AACGGTGGCA GCAGTTCAAC
    ATTCTGGACA GCATGCGGTG CTTCCAACAA GCGCACTATG ATATCCGAAG AAACGATGAC
    ATCATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCGG AGGAGGCTCT GTGGGAACTC
    TCTCTGAAAA TCAAACCTAG GAACATAACA AGGAGAAAAA CAGACCGAGA GGAGAAGACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12668
    Clone ID Related Accession (Same CDS sequence) XM_005625213.2 , XM_005625213.3
    Accession Version XM_005625213.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGGAGT CACCAGCTAT GAAAGATGGA CATCCCAGAG ACCCCTCATC GGTCAGCAGC
    ACACCTGGGA AGGAGAGCAG AGACAGCGAC AGGGCCTCAA GGTCACCAGA TGGTTCAGAG
    TTGGCTCGGT TGGAGGAGGA AGTGGATGAG CTGTCCCTCA TTGACCACAA TGAAATTATG
    GCAAGGCTGA CACTCAAGCA GGAGGGTGAT GATGGGCCAG ACGTCCGTGG AGGGTCTGGA
    GATATCCTAC TGGTGCACGC TACTGAGACA GACAGGAAAG ATTTGGTGTT GTACTGTGAG
    GCCTTCTTGA CCACCTACAG GACATTCATC ACCCCAGAGG AGCTCATCAA GAAGCTGCAG
    TACAGGTACG AGAAGTTCTC TCCCTTCGCT GACACCTTCA AGAAGCGCGT CAGCAAGAAC
    ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT GAGCTCTGCC TAGTGGAGTT GACAGAAGAG
    ATCTTGAAGC TGCTCATGGA ACTGGTCTTT CGCTTGGTGT GCAACGGGGA GCTCAGCCTG
    GCCCGCGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT GCTCAGGTGT
    GCCAACTCCG ACCAGCCCCT GGCAGCCAGG GGGGTAGCTG CCAGGCCAGG GACTTTGCAT
    GACTTTCACA GCCATGAAAT AGCCGAGCAG CTGACACTGC TGGATGCTGA GCTCTTTTAT
    AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAAC AGAATGAGGA GAAAAGCCCC
    AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCGT ACTGGGTCCG GTCAATAATC
    ATGTTACAAG AAAAAGCCCA GGACAGGGAG CGACTGCTAC TGAAGTTCAT CAAGATCATG
    AAGCACTTAC GGAAGCTGAA CAACTTTAAC TCCTACCTGG CCATCCTCTC GGCACTGGAC
    TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG AAGCAGACCT CGGAGGGCCT GGCCGAGTAC
    TGTACACTGA TCGACAGCTC GTCCTCCTTC CGCGCCTACC GGGCCGCCCT GTCCGAGGTG
    GAGCCGCCCT GCATCCCATA CCTGGGGCTT ATCCTGCAGG ACCTCACCTT TGTTCACCTG
    GGAAACCCAG ACTATATTGA CGGGAAGGTG AATTTTTCCA AACGGTGGCA GCAGTTCAAC
    ATTCTGGACA GCATGCGGTG CTTCCAACAA GCGCACTATG ATATCCGAAG AAACGATGAC
    ATCATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCGG AGGAGGCTCT GTGGGAACTC
    TCTCTGAAAA TCAAACCTAG GAACATAACA AGGAGAAAAA CAGACCGAGA GGAGAAGACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625270.1
    CDS353..4075
    Translation

    Target ORF information:

    RefSeq Version XM_005625213.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGTATAAAT CTGTGTTTAG GTCCTACTCC
    CAGGATTTCG TGCCTCACAA CCACGCTTCC GTTCAGCCTT TCCTTCCGCC TACCTCCTCT
    GCCTCTCCAC ACTTCCCAAC TGTCCACCAG TCTCAGAGCT CTGACTTAAC GGTGCCAACC
    GTAGCCAGTC TGCCTCCCAG CACCGTGGAC GGGCCTCTCT CGTCTTCTCA GGAGAGCAGC
    TTTCACGGGA ACACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GCCCCAGACG
    CCTTTGGAGT CACCAGCTAT GAAAGATGGA CATCCCAGAG ACCCCTCATC GGTCAGCAGC
    ACACCTGGGA AGGAGAGCAG AGACAGCGAC AGGGCCTCAA GGTCACCAGA TGGTTCAGAG
    TTGGCTCGGT TGGAGGAGGA AGTGGATGAG CTGTCCCTCA TTGACCACAA TGAAATTATG
    GCAAGGCTGA CACTCAAGCA GGAGGGTGAT GATGGGCCAG ACGTCCGTGG AGGGTCTGGA
    GATATCCTAC TGGTGCACGC TACTGAGACA GACAGGAAAG ATTTGGTGTT GTACTGTGAG
    GCCTTCTTGA CCACCTACAG GACATTCATC ACCCCAGAGG AGCTCATCAA GAAGCTGCAG
    TACAGGTACG AGAAGTTCTC TCCCTTCGCT GACACCTTCA AGAAGCGCGT CAGCAAGAAC
    ACTTTCTTCG TGCTGGTGCG AGTGGTGGAT GAGCTCTGCC TAGTGGAGTT GACAGAAGAG
    ATCTTGAAGC TGCTCATGGA ACTGGTCTTT CGCTTGGTGT GCAACGGGGA GCTCAGCCTG
    GCCCGCGTGC TCCGGAAGAA CATCCTGGAC AAGGTGGACC AGAAGAAGCT GCTCAGGTGT
    GCCAACTCCG ACCAGCCCCT GGCAGCCAGG GGGGTAGCTG CCAGGCCAGG GACTTTGCAT
    GACTTTCACA GCCATGAAAT AGCCGAGCAG CTGACACTGC TGGATGCTGA GCTCTTTTAT
    AAAATAGAGA TTCCTGAGGT TTTGCTTTGG GCAAAAGAAC AGAATGAGGA GAAAAGCCCC
    AACTTGACCC AGTTCACGGA GCACTTCAAC AACATGTCGT ACTGGGTCCG GTCAATAATC
    ATGTTACAAG AAAAAGCCCA GGACAGGGAG CGACTGCTAC TGAAGTTCAT CAAGATCATG
    AAGCACTTAC GGAAGCTGAA CAACTTTAAC TCCTACCTGG CCATCCTCTC GGCACTGGAC
    TCAGCTCCCA TCCGCAGGCT AGAGTGGCAG AAGCAGACCT CGGAGGGCCT GGCCGAGTAC
    TGTACACTGA TCGACAGCTC GTCCTCCTTC CGCGCCTACC GGGCCGCCCT GTCCGAGGTG
    GAGCCGCCCT GCATCCCATA CCTGGGGCTT ATCCTGCAGG ACCTCACCTT TGTTCACCTG
    GGAAACCCAG ACTATATTGA CGGGAAGGTG AATTTTTCCA AACGGTGGCA GCAGTTCAAC
    ATTCTGGACA GCATGCGGTG CTTCCAACAA GCGCACTATG ATATCCGAAG AAACGATGAC
    ATCATAAACT TCTTCAATGA CTTCAGTGAC CACCTGGCGG AGGAGGCTCT GTGGGAACTC
    TCTCTGAAAA TCAAACCTAG GAACATAACA AGGAGAAAAA CAGACCGAGA GGAGAAGACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12669
    Clone ID Related Accession (Same CDS sequence) XM_005625214.2 , XM_005625214.3
    Accession Version XM_005625214.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
    AAACCAGCTG AAGTTTCTGT GAAAAATCCG GAGAAGCCTG TGAGCAAAGA GGCAACAGAC
    AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT
    ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAGAGGCAGA AGAACCTGAG CTGGCTGGAA
    GAGAAGGAGA AGGAAGTTGT TAGTGCCTTG CGCTACTTTA AGACTATCGT GGACAAGATG
    GCTATTGATA AGAAGGTACT GGAGATGCTG CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC
    ATCTTACCCC TGGTGCAGAG CGATCCTCGA ATTCAACACA GCTCAGCCCT CTCCTCCTGC
    TATAGCCGAG TGTACCAAAG TCTTGCCAAC CTCATCCGTT GGTCTGACCA AGTGATGCTG
    GAAGGTGTGA ACTCAGAAGA TAAAGAGACG GTGACAACGG TGAAGGGGGT CATCAAGGCG
    GTACTGGATG GAGTGAAGGA GCTGGTCAGA CTTACCATCG AGAAACAGGG GCATCCATCT
    CCAACCAGCC CAGTGAAACC CAGTTCCCCA GCCTGCAAAC CGGACGGCCA GTCTGAGCTC
    CCCCTGACAG ATCGCGAGGT GGAGATTCTC AACAAGACAA CTGGCATGTC CCAGTCGGCC
    GAGCTACTCC CAGACTCAAC AGATGAGGAG GTTGCGCCCC CTAAGCCCCC CTTACCTGGC
    ATTCGGGTGG TTGATAACAG TCCTCCCCCA GCATTGCCAC CGAAGAAAAG ACAGTCAGCT
    CCATCCCCGA CCCGAGTGGC TGTTGTTGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT
    TTGCCTGTTG GAATTAATAG GCAGGATTTT GATGTGGACT GTTACACACA GAGGCGACTC
    TCAGGAGGCA GCCACTCATA CGGCGGAGAG TCGCCCCGTC TGTCCCCCTG CAGCAGCATA
    GGCAAGCTCA GCAAGTCAGA TGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGCCAGTGC
    TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTATGATC CTGACTACGA ATTCCTTCAG
    CAAGACCTCT CTAATGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT TAGCCCATTG
    CCAGAGTCTT TGGGAGAGTC TGGGTCTCCA TTTCTTGGCC ATCCTTTCCA GTTGCCTCCA
    GCGCCTGGCA GCTGTCCCCA GCCAGAGGGG CCCTTGGCCC CAGGACAGCA GATAGACATG
    CCACCTGCTC TCCCTGAGAA GAAGCGCAGG AGTGCGGCCT CCCAGACCAC GGACAGCTCT
    GGCTGCAGGA TGTCCTACGA GCGGCACCCC TCACAATATG ACAATATCTC GGAAGATGAC
    CTGCAGAACC CAGCCTCGGT CCCGTCCATC CCCTTCACGC CCTTTGCTGC TATTCTCCCC
    TTTCAGCAAG GAGGGTCCTC AGCCTCTGTC GAATTTGTGG GTGATTTCAC TGTTTCTGAA
    TCAACAACGG GTGACCCAGA AAAACCACCT CCTCTACCAG AGAAGAAAAA CAAACACATG
    CTGGCCTACA TGCAGCTGCT GGAGGACTAC TCGGAGCCAC AGCCGTCCAT GTTCTACCAG
    ACGCCACAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTGTACGGC
    TTCAACGACT CATTCAGCAC CGAAGCCCCG CAGGAGCTGG CACCGCCCCC CGCCCTGCCT
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCAC TCAGGGCATC
    AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA ACTTGCCTGT GCCCTCGTAT
    AAATCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCACGC TTCCGTTCAG
    CCTTTCCTTC CGCCTACCTC CTCTGCCTCT CCACACTTCC CAACTGTCCA CCAGTCTCAG
    AGCTCTGACT TAACGGTGCC AACCGTAGCC AGTCTGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGAGAG CAGCTTTCAC GGGAACACTG TCTGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGCCCCA GACGCCTTTG AGTGAAAGCA CCAGCGAGGA GGCTGGTGAG
    GGTGAATACG TCAATCTATA TTCCTCTGGC CAGAGCAGCA GGGAGCTGGC TCACTGTGGA
    GGAGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625271.1
    CDS125..3844
    Translation

    Target ORF information:

    RefSeq Version XM_005625214.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625214.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
    AAACCAGCTG AAGTTTCTGT GAAAAATCCG GAGAAGCCTG TGAGCAAAGA GGCAACAGAC
    AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT
    ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAGAGGCAGA AGAACCTGAG CTGGCTGGAA
    GAGAAGGAGA AGGAAGTTGT TAGTGCCTTG CGCTACTTTA AGACTATCGT GGACAAGATG
    GCTATTGATA AGAAGGTACT GGAGATGCTG CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC
    ATCTTACCCC TGGTGCAGAG CGATCCTCGA ATTCAACACA GCTCAGCCCT CTCCTCCTGC
    TATAGCCGAG TGTACCAAAG TCTTGCCAAC CTCATCCGTT GGTCTGACCA AGTGATGCTG
    GAAGGTGTGA ACTCAGAAGA TAAAGAGACG GTGACAACGG TGAAGGGGGT CATCAAGGCG
    GTACTGGATG GAGTGAAGGA GCTGGTCAGA CTTACCATCG AGAAACAGGG GCATCCATCT
    CCAACCAGCC CAGTGAAACC CAGTTCCCCA GCCTGCAAAC CGGACGGCCA GTCTGAGCTC
    CCCCTGACAG ATCGCGAGGT GGAGATTCTC AACAAGACAA CTGGCATGTC CCAGTCGGCC
    GAGCTACTCC CAGACTCAAC AGATGAGGAG GTTGCGCCCC CTAAGCCCCC CTTACCTGGC
    ATTCGGGTGG TTGATAACAG TCCTCCCCCA GCATTGCCAC CGAAGAAAAG ACAGTCAGCT
    CCATCCCCGA CCCGAGTGGC TGTTGTTGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT
    TTGCCTGTTG GAATTAATAG GCAGGATTTT GATGTGGACT GTTACACACA GAGGCGACTC
    TCAGGAGGCA GCCACTCATA CGGCGGAGAG TCGCCCCGTC TGTCCCCCTG CAGCAGCATA
    GGCAAGCTCA GCAAGTCAGA TGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGCCAGTGC
    TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTATGATC CTGACTACGA ATTCCTTCAG
    CAAGACCTCT CTAATGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT TAGCCCATTG
    CCAGAGTCTT TGGGAGAGTC TGGGTCTCCA TTTCTTGGCC ATCCTTTCCA GTTGCCTCCA
    GCGCCTGGCA GCTGTCCCCA GCCAGAGGGG CCCTTGGCCC CAGGACAGCA GATAGACATG
    CCACCTGCTC TCCCTGAGAA GAAGCGCAGG AGTGCGGCCT CCCAGACCAC GGACAGCTCT
    GGCTGCAGGA TGTCCTACGA GCGGCACCCC TCACAATATG ACAATATCTC GGAAGATGAC
    CTGCAGAACC CAGCCTCGGT CCCGTCCATC CCCTTCACGC CCTTTGCTGC TATTCTCCCC
    TTTCAGCAAG GAGGGTCCTC AGCCTCTGTC GAATTTGTGG GTGATTTCAC TGTTTCTGAA
    TCAACAACGG GTGACCCAGA AAAACCACCT CCTCTACCAG AGAAGAAAAA CAAACACATG
    CTGGCCTACA TGCAGCTGCT GGAGGACTAC TCGGAGCCAC AGCCGTCCAT GTTCTACCAG
    ACGCCACAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTGTACGGC
    TTCAACGACT CATTCAGCAC CGAAGCCCCG CAGGAGCTGG CACCGCCCCC CGCCCTGCCT
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCAC TCAGGGCATC
    AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA ACTTGCCTGT GCCCTCGTAT
    AAATCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCACGC TTCCGTTCAG
    CCTTTCCTTC CGCCTACCTC CTCTGCCTCT CCACACTTCC CAACTGTCCA CCAGTCTCAG
    AGCTCTGACT TAACGGTGCC AACCGTAGCC AGTCTGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGAGAG CAGCTTTCAC GGGAACACTG TCTGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGCCCCA GACGCCTTTG AGTGAAAGCA CCAGCGAGGA GGCTGGTGAG
    GGTGAATACG TCAATCTATA TTCCTCTGGC CAGAGCAGCA GGGAGCTGGC TCACTGTGGA
    GGAGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12669
    Clone ID Related Accession (Same CDS sequence) XM_005625214.2 , XM_005625214.3
    Accession Version XM_005625214.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
    AAACCAGCTG AAGTTTCTGT GAAAAATCCG GAGAAGCCTG TGAGCAAAGA GGCAACAGAC
    AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT
    ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAGAGGCAGA AGAACCTGAG CTGGCTGGAA
    GAGAAGGAGA AGGAAGTTGT TAGTGCCTTG CGCTACTTTA AGACTATCGT GGACAAGATG
    GCTATTGATA AGAAGGTACT GGAGATGCTG CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC
    ATCTTACCCC TGGTGCAGAG CGATCCTCGA ATTCAACACA GCTCAGCCCT CTCCTCCTGC
    TATAGCCGAG TGTACCAAAG TCTTGCCAAC CTCATCCGTT GGTCTGACCA AGTGATGCTG
    GAAGGTGTGA ACTCAGAAGA TAAAGAGACG GTGACAACGG TGAAGGGGGT CATCAAGGCG
    GTACTGGATG GAGTGAAGGA GCTGGTCAGA CTTACCATCG AGAAACAGGG GCATCCATCT
    CCAACCAGCC CAGTGAAACC CAGTTCCCCA GCCTGCAAAC CGGACGGCCA GTCTGAGCTC
    CCCCTGACAG ATCGCGAGGT GGAGATTCTC AACAAGACAA CTGGCATGTC CCAGTCGGCC
    GAGCTACTCC CAGACTCAAC AGATGAGGAG GTTGCGCCCC CTAAGCCCCC CTTACCTGGC
    ATTCGGGTGG TTGATAACAG TCCTCCCCCA GCATTGCCAC CGAAGAAAAG ACAGTCAGCT
    CCATCCCCGA CCCGAGTGGC TGTTGTTGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT
    TTGCCTGTTG GAATTAATAG GCAGGATTTT GATGTGGACT GTTACACACA GAGGCGACTC
    TCAGGAGGCA GCCACTCATA CGGCGGAGAG TCGCCCCGTC TGTCCCCCTG CAGCAGCATA
    GGCAAGCTCA GCAAGTCAGA TGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGCCAGTGC
    TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTATGATC CTGACTACGA ATTCCTTCAG
    CAAGACCTCT CTAATGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT TAGCCCATTG
    CCAGAGTCTT TGGGAGAGTC TGGGTCTCCA TTTCTTGGCC ATCCTTTCCA GTTGCCTCCA
    GCGCCTGGCA GCTGTCCCCA GCCAGAGGGG CCCTTGGCCC CAGGACAGCA GATAGACATG
    CCACCTGCTC TCCCTGAGAA GAAGCGCAGG AGTGCGGCCT CCCAGACCAC GGACAGCTCT
    GGCTGCAGGA TGTCCTACGA GCGGCACCCC TCACAATATG ACAATATCTC GGAAGATGAC
    CTGCAGAACC CAGCCTCGGT CCCGTCCATC CCCTTCACGC CCTTTGCTGC TATTCTCCCC
    TTTCAGCAAG GAGGGTCCTC AGCCTCTGTC GAATTTGTGG GTGATTTCAC TGTTTCTGAA
    TCAACAACGG GTGACCCAGA AAAACCACCT CCTCTACCAG AGAAGAAAAA CAAACACATG
    CTGGCCTACA TGCAGCTGCT GGAGGACTAC TCGGAGCCAC AGCCGTCCAT GTTCTACCAG
    ACGCCACAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTGTACGGC
    TTCAACGACT CATTCAGCAC CGAAGCCCCG CAGGAGCTGG CACCGCCCCC CGCCCTGCCT
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCAC TCAGGGCATC
    AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA ACTTGCCTGT GCCCTCGTAT
    AAATCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCACGC TTCCGTTCAG
    CCTTTCCTTC CGCCTACCTC CTCTGCCTCT CCACACTTCC CAACTGTCCA CCAGTCTCAG
    AGCTCTGACT TAACGGTGCC AACCGTAGCC AGTCTGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGAGAG CAGCTTTCAC GGGAACACTG TCTGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGCCCCA GACGCCTTTG AGTGAAAGCA CCAGCGAGGA GGCTGGTGAG
    GGTGAATACG TCAATCTATA TTCCTCTGGC CAGAGCAGCA GGGAGCTGGC TCACTGTGGA
    GGAGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625271.1
    CDS135..3854
    Translation

    Target ORF information:

    RefSeq Version XM_005625214.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625214.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AGGACAAATT CCACTCACCC AAAATCAAGA GAACGCCATC AAAGAAGGGA 
    AAACCAGCTG AAGTTTCTGT GAAAAATCCG GAGAAGCCTG TGAGCAAAGA GGCAACAGAC
    AGATTTCTAC CAGAGGGCTA CCCTCTCCCC TTGGATCTGG AGCAGCAGGC AGTAGAATTT
    ATGTCCACCA GTGCTGTGGC TTCCAGGTCT CAGAGGCAGA AGAACCTGAG CTGGCTGGAA
    GAGAAGGAGA AGGAAGTTGT TAGTGCCTTG CGCTACTTTA AGACTATCGT GGACAAGATG
    GCTATTGATA AGAAGGTACT GGAGATGCTG CCAGGGTCAG CCAGCAAGGT GCTGGAGGCC
    ATCTTACCCC TGGTGCAGAG CGATCCTCGA ATTCAACACA GCTCAGCCCT CTCCTCCTGC
    TATAGCCGAG TGTACCAAAG TCTTGCCAAC CTCATCCGTT GGTCTGACCA AGTGATGCTG
    GAAGGTGTGA ACTCAGAAGA TAAAGAGACG GTGACAACGG TGAAGGGGGT CATCAAGGCG
    GTACTGGATG GAGTGAAGGA GCTGGTCAGA CTTACCATCG AGAAACAGGG GCATCCATCT
    CCAACCAGCC CAGTGAAACC CAGTTCCCCA GCCTGCAAAC CGGACGGCCA GTCTGAGCTC
    CCCCTGACAG ATCGCGAGGT GGAGATTCTC AACAAGACAA CTGGCATGTC CCAGTCGGCC
    GAGCTACTCC CAGACTCAAC AGATGAGGAG GTTGCGCCCC CTAAGCCCCC CTTACCTGGC
    ATTCGGGTGG TTGATAACAG TCCTCCCCCA GCATTGCCAC CGAAGAAAAG ACAGTCAGCT
    CCATCCCCGA CCCGAGTGGC TGTTGTTGCC CCCATGAGTC GAGCCACCAG TGGCTCCAGT
    TTGCCTGTTG GAATTAATAG GCAGGATTTT GATGTGGACT GTTACACACA GAGGCGACTC
    TCAGGAGGCA GCCACTCATA CGGCGGAGAG TCGCCCCGTC TGTCCCCCTG CAGCAGCATA
    GGCAAGCTCA GCAAGTCAGA TGAGCAGCTG TCCTCTCTGG ACAGGGACAG CGGCCAGTGC
    TCCCGGAACA CAAGCTGTGA AACACTAGAT CATTATGATC CTGACTACGA ATTCCTTCAG
    CAAGACCTCT CTAATGCAGA CCAGATACCT CAGCAAGTGG CCTGTAACCT TAGCCCATTG
    CCAGAGTCTT TGGGAGAGTC TGGGTCTCCA TTTCTTGGCC ATCCTTTCCA GTTGCCTCCA
    GCGCCTGGCA GCTGTCCCCA GCCAGAGGGG CCCTTGGCCC CAGGACAGCA GATAGACATG
    CCACCTGCTC TCCCTGAGAA GAAGCGCAGG AGTGCGGCCT CCCAGACCAC GGACAGCTCT
    GGCTGCAGGA TGTCCTACGA GCGGCACCCC TCACAATATG ACAATATCTC GGAAGATGAC
    CTGCAGAACC CAGCCTCGGT CCCGTCCATC CCCTTCACGC CCTTTGCTGC TATTCTCCCC
    TTTCAGCAAG GAGGGTCCTC AGCCTCTGTC GAATTTGTGG GTGATTTCAC TGTTTCTGAA
    TCAACAACGG GTGACCCAGA AAAACCACCT CCTCTACCAG AGAAGAAAAA CAAACACATG
    CTGGCCTACA TGCAGCTGCT GGAGGACTAC TCGGAGCCAC AGCCGTCCAT GTTCTACCAG
    ACGCCACAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTGTACGGC
    TTCAACGACT CATTCAGCAC CGAAGCCCCG CAGGAGCTGG CACCGCCCCC CGCCCTGCCT
    CCCAAGCAGC GGCAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC
    TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCAC TCAGGGCATC
    AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA ACTTGCCTGT GCCCTCGTAT
    AAATCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCACGC TTCCGTTCAG
    CCTTTCCTTC CGCCTACCTC CTCTGCCTCT CCACACTTCC CAACTGTCCA CCAGTCTCAG
    AGCTCTGACT TAACGGTGCC AACCGTAGCC AGTCTGCCTC CCAGCACCGT GGACGGGCCT
    CTCTCGTCTT CTCAGGAGAG CAGCTTTCAC GGGAACACTG TCTGCCTTCC TTCCGAAACC
    TCTTTCACTG ACTCGCCCCA GACGCCTTTG AGTGAAAGCA CCAGCGAGGA GGCTGGTGAG
    GGTGAATACG TCAATCTATA TTCCTCTGGC CAGAGCAGCA GGGAGCTGGC TCACTGTGGA
    GGAGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12670
    Clone ID Related Accession (Same CDS sequence) XM_005625215.2 , XM_005625215.3
    Accession Version XM_005625215.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625272.1
    CDS355..4074
    Translation

    Target ORF information:

    RefSeq Version XM_005625215.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625215.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12670
    Clone ID Related Accession (Same CDS sequence) XM_005625215.2 , XM_005625215.3
    Accession Version XM_005625215.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625215.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625272.1
    CDS353..4072
    Translation

    Target ORF information:

    RefSeq Version XM_005625215.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625215.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGCCTCC
    TATGCTGCTT CTTCCTTCTC CTCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGGCCTTC
    ACCCCCGAGG ACGGCAGTGC CACTCAGGGC ATCAGTGTGT CCGTCTCTAA CTCCTTTCTC
    AGCCGGCATG GCAACTTGCC TGTGCCCTCG TATAAATCTG TGTTTAGGTC CTACTCCCAG
    GATTTCGTGC CTCACAACCA CGCTTCCGTT CAGCCTTTCC TTCCGCCTAC CTCCTCTGCC
    TCTCCACACT TCCCAACTGT CCACCAGTCT CAGAGCTCTG ACTTAACGGT GCCAACCGTA
    GCCAGTCTGC CTCCCAGCAC CGTGGACGGG CCTCTCTCGT CTTCTCAGGA GAGCAGCTTT
    CACGGGAACA CTGTCTGCCT TCCTTCCGAA ACCTCTTTCA CTGACTCGCC CCAGACGCCT
    TTGGAGTCAC CAGCTATGAA AGATGGACAT CCCAGAGACC CCTCATCGGT CAGCAGCACA
    CCTGGGAAGG AGAGCAGAGA CAGCGACAGG GCCTCAAGGT CACCAGATGG TTCAGAGTTG
    GCTCGGTTGG AGGAGGAAGT GGATGAGCTG TCCCTCATTG ACCACAATGA AATTATGGCA
    AGGCTGACAC TCAAGCAGGA GGGTGATGAT GGGCCAGACG TCCGTGGAGG GTCTGGAGAT
    ATCCTACTGG TGCACGCTAC TGAGACAGAC AGGAAAGATT TGGTGTTGTA CTGTGAGGCC
    TTCTTGACCA CCTACAGGAC ATTCATCACC CCAGAGGAGC TCATCAAGAA GCTGCAGTAC
    AGGTACGAGA AGTTCTCTCC CTTCGCTGAC ACCTTCAAGA AGCGCGTCAG CAAGAACACT
    TTCTTCGTGC TGGTGCGAGT GGTGGATGAG CTCTGCCTAG TGGAGTTGAC AGAAGAGATC
    TTGAAGCTGC TCATGGAACT GGTCTTTCGC TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC
    CGCGTGCTCC GGAAGAACAT CCTGGACAAG GTGGACCAGA AGAAGCTGCT CAGGTGTGCC
    AACTCCGACC AGCCCCTGGC AGCCAGGGGG GTAGCTGCCA GGCCAGGGAC TTTGCATGAC
    TTTCACAGCC ATGAAATAGC CGAGCAGCTG ACACTGCTGG ATGCTGAGCT CTTTTATAAA
    ATAGAGATTC CTGAGGTTTT GCTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC
    TTGACCCAGT TCACGGAGCA CTTCAACAAC ATGTCGTACT GGGTCCGGTC AATAATCATG
    TTACAAGAAA AAGCCCAGGA CAGGGAGCGA CTGCTACTGA AGTTCATCAA GATCATGAAG
    CACTTACGGA AGCTGAACAA CTTTAACTCC TACCTGGCCA TCCTCTCGGC ACTGGACTCA
    GCTCCCATCC GCAGGCTAGA GTGGCAGAAG CAGACCTCGG AGGGCCTGGC CGAGTACTGT
    ACACTGATCG ACAGCTCGTC CTCCTTCCGC GCCTACCGGG CCGCCCTGTC CGAGGTGGAG
    CCGCCCTGCA TCCCATACCT GGGGCTTATC CTGCAGGACC TCACCTTTGT TCACCTGGGA
    AACCCAGACT ATATTGACGG GAAGGTGAAT TTTTCCAAAC GGTGGCAGCA GTTCAACATT
    CTGGACAGCA TGCGGTGCTT CCAACAAGCG CACTATGATA TCCGAAGAAA CGATGACATC
    ATAAACTTCT TCAATGACTT CAGTGACCAC CTGGCGGAGG AGGCTCTGTG GGAACTCTCT
    CTGAAAATCA AACCTAGGAA CATAACAAGG AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12671
    Clone ID Related Accession (Same CDS sequence) XM_005625216.3 , XM_005625216.2
    Accession Version XM_005625216.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAAACCTG AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT
    GTTAGTGCCT TGCGCTACTT TAAGACTATC GTGGACAAGA TGGCTATTGA TAAGAAGGTA
    CTGGAGATGC TGCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG
    AGCGATCCTC GAATTCAACA CAGCTCAGCC CTCTCCTCCT GCTATAGCCG AGTGTACCAA
    AGTCTTGCCA ACCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA
    GATAAAGAGA CGGTGACAAC GGTGAAGGGG GTCATCAAGG CGGTACTGGA TGGAGTGAAG
    GAGCTGGTCA GACTTACCAT CGAGAAACAG GGGCATCCAT CTCCAACCAG CCCAGTGAAA
    CCCAGTTCCC CAGCCTGCAA ACCGGACGGC CAGTCTGAGC TCCCCCTGAC AGATCGCGAG
    GTGGAGATTC TCAACAAGAC AACTGGCATG TCCCAGTCGG CCGAGCTACT CCCAGACTCA
    ACAGATGAGG AGGTTGCGCC CCCTAAGCCC CCCTTACCTG GCATTCGGGT GGTTGATAAC
    AGTCCTCCCC CAGCATTGCC ACCGAAGAAA AGACAGTCAG CTCCATCCCC GACCCGAGTG
    GCTGTTGTTG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT
    AGGCAGGATT TTGATGTGGA CTGTTACACA CAGAGGCGAC TCTCAGGAGG CAGCCACTCA
    TACGGCGGAG AGTCGCCCCG TCTGTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA
    GATGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGCCAGT GCTCCCGGAA CACAAGCTGT
    GAAACACTAG ATCATTATGA TCCTGACTAC GAATTCCTTC AGCAAGACCT CTCTAATGCA
    GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG
    TCTGGGTCTC CATTTCTTGG CCATCCTTTC CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC
    CAGCCAGAGG GGCCCTTGGC CCCAGGACAG CAGATAGACA TGCCACCTGC TCTCCCTGAG
    AAGAAGCGCA GGAGTGCGGC CTCCCAGACC ACGGACAGCT CTGGCTGCAG GATGTCCTAC
    GAGCGGCACC CCTCACAATA TGACAATATC TCGGAAGATG ACCTGCAGAA CCCAGCCTCG
    GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT GCTATTCTCC CCTTTCAGCA AGGAGGGTCC
    TCAGCCTCTG TCGAATTTGT GGGTGATTTC ACTGTTTCTG AATCAACAAC GGGTGACCCA
    GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG
    CTGGAGGACT ACTCGGAGCC ACAGCCGTCC ATGTTCTACC AGACGCCACA GAACGAGCAC
    ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTGTACG GCTTCAACGA CTCATTCAGC
    ACCGAAGCCC CGCAGGAGCT GGCACCGCCC CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG
    CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA
    GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC
    TCCTTTCTCA GCCGGCATGG CAACTTGCCT GTGCCCTCGT ATAAATCTGT GTTTAGGTCC
    TACTCCCAGG ATTTCGTGCC TCACAACCAC GCTTCCGTTC AGCCTTTCCT TCCGCCTACC
    TCCTCTGCCT CTCCACACTT CCCAACTGTC CACCAGTCTC AGAGCTCTGA CTTAACGGTG
    CCAACCGTAG CCAGTCTGCC TCCCAGCACC GTGGACGGGC CTCTCTCGTC TTCTCAGGAG
    AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC
    CAGACGCCTT TGAGTGAAAG CACCAGCGAG GAGGCTGGTG AGGGTGAATA CGTCAATCTA
    TATTCCTCTG GCCAGAGCAG CAGGGAGCTG GCTCACTGTG GAGGAGAGTC ACCAGCTATG
    AAAGATGGAC ATCCCAGAGA CCCCTCATCG GTCAGCAGCA CACCTGGGAA GGAGAGCAGA
    GACAGCGACA GGGCCTCAAG GTCACCAGAT GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA
    GTGGATGAGC TGTCCCTCAT TGACCACAAT GAAATTATGG CAAGGCTGAC ACTCAAGCAG
    GAGGGTGATG ATGGGCCAGA CGTCCGTGGA GGGTCTGGAG ATATCCTACT GGTGCACGCT
    ACTGAGACAG ACAGGAAAGA TTTGGTGTTG TACTGTGAGG CCTTCTTGAC CACCTACAGG
    ACATTCATCA CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGGTACGA GAAGTTCTCT
    CCCTTCGCTG ACACCTTCAA GAAGCGCGTC AGCAAGAACA CTTTCTTCGT GCTGGTGCGA
    GTGGTGGATG AGCTCTGCCT AGTGGAGTTG ACAGAAGAGA TCTTGAAGCT GCTCATGGAA
    CTGGTCTTTC GCTTGGTGTG CAACGGGGAG CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC
    ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCAACTCCGA CCAGCCCCTG
    GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG ACTTTGCATG ACTTTCACAG CCATGAAATA
    GCCGAGCAGC TGACACTGCT GGATGCTGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT
    TTGCTTTGGG CAAAAGAACA GAATGAGGAG AAAAGCCCCA ACTTGACCCA GTTCACGGAG
    CACTTCAACA ACATGTCGTA CTGGGTCCGG TCAATAATCA TGTTACAAGA AAAAGCCCAG
    GACAGGGAGC GACTGCTACT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC
    AACTTTAACT CCTACCTGGC CATCCTCTCG GCACTGGACT CAGCTCCCAT CCGCAGGCTA
    GAGTGGCAGA AGCAGACCTC GGAGGGCCTG GCCGAGTACT GTACACTGAT CGACAGCTCG
    TCCTCCTTCC GCGCCTACCG GGCCGCCCTG TCCGAGGTGG AGCCGCCCTG CATCCCATAC
    CTGGGGCTTA TCCTGCAGGA CCTCACCTTT GTTCACCTGG GAAACCCAGA CTATATTGAC
    GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG CAGTTCAACA TTCTGGACAG CATGCGGTGC
    TTCCAACAAG CGCACTATGA TATCCGAAGA AACGATGACA TCATAAACTT CTTCAATGAC
    TTCAGTGACC ACCTGGCGGA GGAGGCTCTG TGGGAACTCT CTCTGAAAAT CAAACCTAGG
    AACATAACAA GGAGAAAAAC AGACCGAGAG GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625273.1
    CDS354..4055
    Translation

    Target ORF information:

    RefSeq Version XM_005625216.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625216.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAAACCTG AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT
    GTTAGTGCCT TGCGCTACTT TAAGACTATC GTGGACAAGA TGGCTATTGA TAAGAAGGTA
    CTGGAGATGC TGCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG
    AGCGATCCTC GAATTCAACA CAGCTCAGCC CTCTCCTCCT GCTATAGCCG AGTGTACCAA
    AGTCTTGCCA ACCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA
    GATAAAGAGA CGGTGACAAC GGTGAAGGGG GTCATCAAGG CGGTACTGGA TGGAGTGAAG
    GAGCTGGTCA GACTTACCAT CGAGAAACAG GGGCATCCAT CTCCAACCAG CCCAGTGAAA
    CCCAGTTCCC CAGCCTGCAA ACCGGACGGC CAGTCTGAGC TCCCCCTGAC AGATCGCGAG
    GTGGAGATTC TCAACAAGAC AACTGGCATG TCCCAGTCGG CCGAGCTACT CCCAGACTCA
    ACAGATGAGG AGGTTGCGCC CCCTAAGCCC CCCTTACCTG GCATTCGGGT GGTTGATAAC
    AGTCCTCCCC CAGCATTGCC ACCGAAGAAA AGACAGTCAG CTCCATCCCC GACCCGAGTG
    GCTGTTGTTG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT
    AGGCAGGATT TTGATGTGGA CTGTTACACA CAGAGGCGAC TCTCAGGAGG CAGCCACTCA
    TACGGCGGAG AGTCGCCCCG TCTGTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA
    GATGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGCCAGT GCTCCCGGAA CACAAGCTGT
    GAAACACTAG ATCATTATGA TCCTGACTAC GAATTCCTTC AGCAAGACCT CTCTAATGCA
    GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG
    TCTGGGTCTC CATTTCTTGG CCATCCTTTC CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC
    CAGCCAGAGG GGCCCTTGGC CCCAGGACAG CAGATAGACA TGCCACCTGC TCTCCCTGAG
    AAGAAGCGCA GGAGTGCGGC CTCCCAGACC ACGGACAGCT CTGGCTGCAG GATGTCCTAC
    GAGCGGCACC CCTCACAATA TGACAATATC TCGGAAGATG ACCTGCAGAA CCCAGCCTCG
    GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT GCTATTCTCC CCTTTCAGCA AGGAGGGTCC
    TCAGCCTCTG TCGAATTTGT GGGTGATTTC ACTGTTTCTG AATCAACAAC GGGTGACCCA
    GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG
    CTGGAGGACT ACTCGGAGCC ACAGCCGTCC ATGTTCTACC AGACGCCACA GAACGAGCAC
    ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTGTACG GCTTCAACGA CTCATTCAGC
    ACCGAAGCCC CGCAGGAGCT GGCACCGCCC CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG
    CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA
    GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC
    TCCTTTCTCA GCCGGCATGG CAACTTGCCT GTGCCCTCGT ATAAATCTGT GTTTAGGTCC
    TACTCCCAGG ATTTCGTGCC TCACAACCAC GCTTCCGTTC AGCCTTTCCT TCCGCCTACC
    TCCTCTGCCT CTCCACACTT CCCAACTGTC CACCAGTCTC AGAGCTCTGA CTTAACGGTG
    CCAACCGTAG CCAGTCTGCC TCCCAGCACC GTGGACGGGC CTCTCTCGTC TTCTCAGGAG
    AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC
    CAGACGCCTT TGAGTGAAAG CACCAGCGAG GAGGCTGGTG AGGGTGAATA CGTCAATCTA
    TATTCCTCTG GCCAGAGCAG CAGGGAGCTG GCTCACTGTG GAGGAGAGTC ACCAGCTATG
    AAAGATGGAC ATCCCAGAGA CCCCTCATCG GTCAGCAGCA CACCTGGGAA GGAGAGCAGA
    GACAGCGACA GGGCCTCAAG GTCACCAGAT GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA
    GTGGATGAGC TGTCCCTCAT TGACCACAAT GAAATTATGG CAAGGCTGAC ACTCAAGCAG
    GAGGGTGATG ATGGGCCAGA CGTCCGTGGA GGGTCTGGAG ATATCCTACT GGTGCACGCT
    ACTGAGACAG ACAGGAAAGA TTTGGTGTTG TACTGTGAGG CCTTCTTGAC CACCTACAGG
    ACATTCATCA CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGGTACGA GAAGTTCTCT
    CCCTTCGCTG ACACCTTCAA GAAGCGCGTC AGCAAGAACA CTTTCTTCGT GCTGGTGCGA
    GTGGTGGATG AGCTCTGCCT AGTGGAGTTG ACAGAAGAGA TCTTGAAGCT GCTCATGGAA
    CTGGTCTTTC GCTTGGTGTG CAACGGGGAG CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC
    ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCAACTCCGA CCAGCCCCTG
    GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG ACTTTGCATG ACTTTCACAG CCATGAAATA
    GCCGAGCAGC TGACACTGCT GGATGCTGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT
    TTGCTTTGGG CAAAAGAACA GAATGAGGAG AAAAGCCCCA ACTTGACCCA GTTCACGGAG
    CACTTCAACA ACATGTCGTA CTGGGTCCGG TCAATAATCA TGTTACAAGA AAAAGCCCAG
    GACAGGGAGC GACTGCTACT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC
    AACTTTAACT CCTACCTGGC CATCCTCTCG GCACTGGACT CAGCTCCCAT CCGCAGGCTA
    GAGTGGCAGA AGCAGACCTC GGAGGGCCTG GCCGAGTACT GTACACTGAT CGACAGCTCG
    TCCTCCTTCC GCGCCTACCG GGCCGCCCTG TCCGAGGTGG AGCCGCCCTG CATCCCATAC
    CTGGGGCTTA TCCTGCAGGA CCTCACCTTT GTTCACCTGG GAAACCCAGA CTATATTGAC
    GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG CAGTTCAACA TTCTGGACAG CATGCGGTGC
    TTCCAACAAG CGCACTATGA TATCCGAAGA AACGATGACA TCATAAACTT CTTCAATGAC
    TTCAGTGACC ACCTGGCGGA GGAGGCTCTG TGGGAACTCT CTCTGAAAAT CAAACCTAGG
    AACATAACAA GGAGAAAAAC AGACCGAGAG GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12671
    Clone ID Related Accession (Same CDS sequence) XM_005625216.3 , XM_005625216.2
    Accession Version XM_005625216.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAAACCTG AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT
    GTTAGTGCCT TGCGCTACTT TAAGACTATC GTGGACAAGA TGGCTATTGA TAAGAAGGTA
    CTGGAGATGC TGCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG
    AGCGATCCTC GAATTCAACA CAGCTCAGCC CTCTCCTCCT GCTATAGCCG AGTGTACCAA
    AGTCTTGCCA ACCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA
    GATAAAGAGA CGGTGACAAC GGTGAAGGGG GTCATCAAGG CGGTACTGGA TGGAGTGAAG
    GAGCTGGTCA GACTTACCAT CGAGAAACAG GGGCATCCAT CTCCAACCAG CCCAGTGAAA
    CCCAGTTCCC CAGCCTGCAA ACCGGACGGC CAGTCTGAGC TCCCCCTGAC AGATCGCGAG
    GTGGAGATTC TCAACAAGAC AACTGGCATG TCCCAGTCGG CCGAGCTACT CCCAGACTCA
    ACAGATGAGG AGGTTGCGCC CCCTAAGCCC CCCTTACCTG GCATTCGGGT GGTTGATAAC
    AGTCCTCCCC CAGCATTGCC ACCGAAGAAA AGACAGTCAG CTCCATCCCC GACCCGAGTG
    GCTGTTGTTG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT
    AGGCAGGATT TTGATGTGGA CTGTTACACA CAGAGGCGAC TCTCAGGAGG CAGCCACTCA
    TACGGCGGAG AGTCGCCCCG TCTGTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA
    GATGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGCCAGT GCTCCCGGAA CACAAGCTGT
    GAAACACTAG ATCATTATGA TCCTGACTAC GAATTCCTTC AGCAAGACCT CTCTAATGCA
    GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG
    TCTGGGTCTC CATTTCTTGG CCATCCTTTC CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC
    CAGCCAGAGG GGCCCTTGGC CCCAGGACAG CAGATAGACA TGCCACCTGC TCTCCCTGAG
    AAGAAGCGCA GGAGTGCGGC CTCCCAGACC ACGGACAGCT CTGGCTGCAG GATGTCCTAC
    GAGCGGCACC CCTCACAATA TGACAATATC TCGGAAGATG ACCTGCAGAA CCCAGCCTCG
    GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT GCTATTCTCC CCTTTCAGCA AGGAGGGTCC
    TCAGCCTCTG TCGAATTTGT GGGTGATTTC ACTGTTTCTG AATCAACAAC GGGTGACCCA
    GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG
    CTGGAGGACT ACTCGGAGCC ACAGCCGTCC ATGTTCTACC AGACGCCACA GAACGAGCAC
    ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTGTACG GCTTCAACGA CTCATTCAGC
    ACCGAAGCCC CGCAGGAGCT GGCACCGCCC CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG
    CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA
    GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC
    TCCTTTCTCA GCCGGCATGG CAACTTGCCT GTGCCCTCGT ATAAATCTGT GTTTAGGTCC
    TACTCCCAGG ATTTCGTGCC TCACAACCAC GCTTCCGTTC AGCCTTTCCT TCCGCCTACC
    TCCTCTGCCT CTCCACACTT CCCAACTGTC CACCAGTCTC AGAGCTCTGA CTTAACGGTG
    CCAACCGTAG CCAGTCTGCC TCCCAGCACC GTGGACGGGC CTCTCTCGTC TTCTCAGGAG
    AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC
    CAGACGCCTT TGAGTGAAAG CACCAGCGAG GAGGCTGGTG AGGGTGAATA CGTCAATCTA
    TATTCCTCTG GCCAGAGCAG CAGGGAGCTG GCTCACTGTG GAGGAGAGTC ACCAGCTATG
    AAAGATGGAC ATCCCAGAGA CCCCTCATCG GTCAGCAGCA CACCTGGGAA GGAGAGCAGA
    GACAGCGACA GGGCCTCAAG GTCACCAGAT GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA
    GTGGATGAGC TGTCCCTCAT TGACCACAAT GAAATTATGG CAAGGCTGAC ACTCAAGCAG
    GAGGGTGATG ATGGGCCAGA CGTCCGTGGA GGGTCTGGAG ATATCCTACT GGTGCACGCT
    ACTGAGACAG ACAGGAAAGA TTTGGTGTTG TACTGTGAGG CCTTCTTGAC CACCTACAGG
    ACATTCATCA CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGGTACGA GAAGTTCTCT
    CCCTTCGCTG ACACCTTCAA GAAGCGCGTC AGCAAGAACA CTTTCTTCGT GCTGGTGCGA
    GTGGTGGATG AGCTCTGCCT AGTGGAGTTG ACAGAAGAGA TCTTGAAGCT GCTCATGGAA
    CTGGTCTTTC GCTTGGTGTG CAACGGGGAG CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC
    ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCAACTCCGA CCAGCCCCTG
    GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG ACTTTGCATG ACTTTCACAG CCATGAAATA
    GCCGAGCAGC TGACACTGCT GGATGCTGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT
    TTGCTTTGGG CAAAAGAACA GAATGAGGAG AAAAGCCCCA ACTTGACCCA GTTCACGGAG
    CACTTCAACA ACATGTCGTA CTGGGTCCGG TCAATAATCA TGTTACAAGA AAAAGCCCAG
    GACAGGGAGC GACTGCTACT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC
    AACTTTAACT CCTACCTGGC CATCCTCTCG GCACTGGACT CAGCTCCCAT CCGCAGGCTA
    GAGTGGCAGA AGCAGACCTC GGAGGGCCTG GCCGAGTACT GTACACTGAT CGACAGCTCG
    TCCTCCTTCC GCGCCTACCG GGCCGCCCTG TCCGAGGTGG AGCCGCCCTG CATCCCATAC
    CTGGGGCTTA TCCTGCAGGA CCTCACCTTT GTTCACCTGG GAAACCCAGA CTATATTGAC
    GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG CAGTTCAACA TTCTGGACAG CATGCGGTGC
    TTCCAACAAG CGCACTATGA TATCCGAAGA AACGATGACA TCATAAACTT CTTCAATGAC
    TTCAGTGACC ACCTGGCGGA GGAGGCTCTG TGGGAACTCT CTCTGAAAAT CAAACCTAGG
    AACATAACAA GGAGAAAAAC AGACCGAGAG GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625273.1
    CDS355..4056
    Translation

    Target ORF information:

    RefSeq Version XM_005625216.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625216.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAAACCTG AGCTGGCTGG AAGAGAAGGA GAAGGAAGTT
    GTTAGTGCCT TGCGCTACTT TAAGACTATC GTGGACAAGA TGGCTATTGA TAAGAAGGTA
    CTGGAGATGC TGCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG
    AGCGATCCTC GAATTCAACA CAGCTCAGCC CTCTCCTCCT GCTATAGCCG AGTGTACCAA
    AGTCTTGCCA ACCTCATCCG TTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA
    GATAAAGAGA CGGTGACAAC GGTGAAGGGG GTCATCAAGG CGGTACTGGA TGGAGTGAAG
    GAGCTGGTCA GACTTACCAT CGAGAAACAG GGGCATCCAT CTCCAACCAG CCCAGTGAAA
    CCCAGTTCCC CAGCCTGCAA ACCGGACGGC CAGTCTGAGC TCCCCCTGAC AGATCGCGAG
    GTGGAGATTC TCAACAAGAC AACTGGCATG TCCCAGTCGG CCGAGCTACT CCCAGACTCA
    ACAGATGAGG AGGTTGCGCC CCCTAAGCCC CCCTTACCTG GCATTCGGGT GGTTGATAAC
    AGTCCTCCCC CAGCATTGCC ACCGAAGAAA AGACAGTCAG CTCCATCCCC GACCCGAGTG
    GCTGTTGTTG CCCCCATGAG TCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATTAAT
    AGGCAGGATT TTGATGTGGA CTGTTACACA CAGAGGCGAC TCTCAGGAGG CAGCCACTCA
    TACGGCGGAG AGTCGCCCCG TCTGTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA
    GATGAGCAGC TGTCCTCTCT GGACAGGGAC AGCGGCCAGT GCTCCCGGAA CACAAGCTGT
    GAAACACTAG ATCATTATGA TCCTGACTAC GAATTCCTTC AGCAAGACCT CTCTAATGCA
    GACCAGATAC CTCAGCAAGT GGCCTGTAAC CTTAGCCCAT TGCCAGAGTC TTTGGGAGAG
    TCTGGGTCTC CATTTCTTGG CCATCCTTTC CAGTTGCCTC CAGCGCCTGG CAGCTGTCCC
    CAGCCAGAGG GGCCCTTGGC CCCAGGACAG CAGATAGACA TGCCACCTGC TCTCCCTGAG
    AAGAAGCGCA GGAGTGCGGC CTCCCAGACC ACGGACAGCT CTGGCTGCAG GATGTCCTAC
    GAGCGGCACC CCTCACAATA TGACAATATC TCGGAAGATG ACCTGCAGAA CCCAGCCTCG
    GTCCCGTCCA TCCCCTTCAC GCCCTTTGCT GCTATTCTCC CCTTTCAGCA AGGAGGGTCC
    TCAGCCTCTG TCGAATTTGT GGGTGATTTC ACTGTTTCTG AATCAACAAC GGGTGACCCA
    GAAAAACCAC CTCCTCTACC AGAGAAGAAA AACAAACACA TGCTGGCCTA CATGCAGCTG
    CTGGAGGACT ACTCGGAGCC ACAGCCGTCC ATGTTCTACC AGACGCCACA GAACGAGCAC
    ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTGTACG GCTTCAACGA CTCATTCAGC
    ACCGAAGCCC CGCAGGAGCT GGCACCGCCC CCCGCCCTGC CTCCCAAGCA GCGGCAGCTG
    CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC TCTGTCTCCT ACTGTGTCCA GCAAACTAAA
    GTGGCCTTCA CCCCCGAGGA CGGCAGTGCC ACTCAGGGCA TCAGTGTGTC CGTCTCTAAC
    TCCTTTCTCA GCCGGCATGG CAACTTGCCT GTGCCCTCGT ATAAATCTGT GTTTAGGTCC
    TACTCCCAGG ATTTCGTGCC TCACAACCAC GCTTCCGTTC AGCCTTTCCT TCCGCCTACC
    TCCTCTGCCT CTCCACACTT CCCAACTGTC CACCAGTCTC AGAGCTCTGA CTTAACGGTG
    CCAACCGTAG CCAGTCTGCC TCCCAGCACC GTGGACGGGC CTCTCTCGTC TTCTCAGGAG
    AGCAGCTTTC ACGGGAACAC TGTCTGCCTT CCTTCCGAAA CCTCTTTCAC TGACTCGCCC
    CAGACGCCTT TGAGTGAAAG CACCAGCGAG GAGGCTGGTG AGGGTGAATA CGTCAATCTA
    TATTCCTCTG GCCAGAGCAG CAGGGAGCTG GCTCACTGTG GAGGAGAGTC ACCAGCTATG
    AAAGATGGAC ATCCCAGAGA CCCCTCATCG GTCAGCAGCA CACCTGGGAA GGAGAGCAGA
    GACAGCGACA GGGCCTCAAG GTCACCAGAT GGTTCAGAGT TGGCTCGGTT GGAGGAGGAA
    GTGGATGAGC TGTCCCTCAT TGACCACAAT GAAATTATGG CAAGGCTGAC ACTCAAGCAG
    GAGGGTGATG ATGGGCCAGA CGTCCGTGGA GGGTCTGGAG ATATCCTACT GGTGCACGCT
    ACTGAGACAG ACAGGAAAGA TTTGGTGTTG TACTGTGAGG CCTTCTTGAC CACCTACAGG
    ACATTCATCA CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGGTACGA GAAGTTCTCT
    CCCTTCGCTG ACACCTTCAA GAAGCGCGTC AGCAAGAACA CTTTCTTCGT GCTGGTGCGA
    GTGGTGGATG AGCTCTGCCT AGTGGAGTTG ACAGAAGAGA TCTTGAAGCT GCTCATGGAA
    CTGGTCTTTC GCTTGGTGTG CAACGGGGAG CTCAGCCTGG CCCGCGTGCT CCGGAAGAAC
    ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCAACTCCGA CCAGCCCCTG
    GCAGCCAGGG GGGTAGCTGC CAGGCCAGGG ACTTTGCATG ACTTTCACAG CCATGAAATA
    GCCGAGCAGC TGACACTGCT GGATGCTGAG CTCTTTTATA AAATAGAGAT TCCTGAGGTT
    TTGCTTTGGG CAAAAGAACA GAATGAGGAG AAAAGCCCCA ACTTGACCCA GTTCACGGAG
    CACTTCAACA ACATGTCGTA CTGGGTCCGG TCAATAATCA TGTTACAAGA AAAAGCCCAG
    GACAGGGAGC GACTGCTACT GAAGTTCATC AAGATCATGA AGCACTTACG GAAGCTGAAC
    AACTTTAACT CCTACCTGGC CATCCTCTCG GCACTGGACT CAGCTCCCAT CCGCAGGCTA
    GAGTGGCAGA AGCAGACCTC GGAGGGCCTG GCCGAGTACT GTACACTGAT CGACAGCTCG
    TCCTCCTTCC GCGCCTACCG GGCCGCCCTG TCCGAGGTGG AGCCGCCCTG CATCCCATAC
    CTGGGGCTTA TCCTGCAGGA CCTCACCTTT GTTCACCTGG GAAACCCAGA CTATATTGAC
    GGGAAGGTGA ATTTTTCCAA ACGGTGGCAG CAGTTCAACA TTCTGGACAG CATGCGGTGC
    TTCCAACAAG CGCACTATGA TATCCGAAGA AACGATGACA TCATAAACTT CTTCAATGAC
    TTCAGTGACC ACCTGGCGGA GGAGGCTCTG TGGGAACTCT CTCTGAAAAT CAAACCTAGG
    AACATAACAA GGAGAAAAAC AGACCGAGAG GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12673
    Clone ID Related Accession (Same CDS sequence) XM_005625218.2 , XM_005625218.3
    Accession Version XM_005625218.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625218.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGGAGTCAC CAGCTATGAA AGATGGACAT
    CCCAGAGACC CCTCATCGGT CAGCAGCACA CCTGGGAAGG AGAGCAGAGA CAGCGACAGG
    GCCTCAAGGT CACCAGATGG TTCAGAGTTG GCTCGGTTGG AGGAGGAAGT GGATGAGCTG
    TCCCTCATTG ACCACAATGA AATTATGGCA AGGCTGACAC TCAAGCAGGA GGGTGATGAT
    GGGCCAGACG TCCGTGGAGG GTCTGGAGAT ATCCTACTGG TGCACGCTAC TGAGACAGAC
    AGGAAAGATT TGGTGTTGTA CTGTGAGGCC TTCTTGACCA CCTACAGGAC ATTCATCACC
    CCAGAGGAGC TCATCAAGAA GCTGCAGTAC AGGTACGAGA AGTTCTCTCC CTTCGCTGAC
    ACCTTCAAGA AGCGCGTCAG CAAGAACACT TTCTTCGTGC TGGTGCGAGT GGTGGATGAG
    CTCTGCCTAG TGGAGTTGAC AGAAGAGATC TTGAAGCTGC TCATGGAACT GGTCTTTCGC
    TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC CGCGTGCTCC GGAAGAACAT CCTGGACAAG
    GTGGACCAGA AGAAGCTGCT CAGGTGTGCC AACTCCGACC AGCCCCTGGC AGCCAGGGGG
    GTAGCTGCCA GGCCAGGGAC TTTGCATGAC TTTCACAGCC ATGAAATAGC CGAGCAGCTG
    ACACTGCTGG ATGCTGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCA
    AAAGAACAGA ATGAGGAGAA AAGCCCCAAC TTGACCCAGT TCACGGAGCA CTTCAACAAC
    ATGTCGTACT GGGTCCGGTC AATAATCATG TTACAAGAAA AAGCCCAGGA CAGGGAGCGA
    CTGCTACTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTTAACTCC
    TACCTGGCCA TCCTCTCGGC ACTGGACTCA GCTCCCATCC GCAGGCTAGA GTGGCAGAAG
    CAGACCTCGG AGGGCCTGGC CGAGTACTGT ACACTGATCG ACAGCTCGTC CTCCTTCCGC
    GCCTACCGGG CCGCCCTGTC CGAGGTGGAG CCGCCCTGCA TCCCATACCT GGGGCTTATC
    CTGCAGGACC TCACCTTTGT TCACCTGGGA AACCCAGACT ATATTGACGG GAAGGTGAAT
    TTTTCCAAAC GGTGGCAGCA GTTCAACATT CTGGACAGCA TGCGGTGCTT CCAACAAGCG
    CACTATGATA TCCGAAGAAA CGATGACATC ATAAACTTCT TCAATGACTT CAGTGACCAC
    CTGGCGGAGG AGGCTCTGTG GGAACTCTCT CTGAAAATCA AACCTAGGAA CATAACAAGG
    AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625275.1
    CDS355..3804
    Translation

    Target ORF information:

    RefSeq Version XM_005625218.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625218.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGGAGTCAC CAGCTATGAA AGATGGACAT
    CCCAGAGACC CCTCATCGGT CAGCAGCACA CCTGGGAAGG AGAGCAGAGA CAGCGACAGG
    GCCTCAAGGT CACCAGATGG TTCAGAGTTG GCTCGGTTGG AGGAGGAAGT GGATGAGCTG
    TCCCTCATTG ACCACAATGA AATTATGGCA AGGCTGACAC TCAAGCAGGA GGGTGATGAT
    GGGCCAGACG TCCGTGGAGG GTCTGGAGAT ATCCTACTGG TGCACGCTAC TGAGACAGAC
    AGGAAAGATT TGGTGTTGTA CTGTGAGGCC TTCTTGACCA CCTACAGGAC ATTCATCACC
    CCAGAGGAGC TCATCAAGAA GCTGCAGTAC AGGTACGAGA AGTTCTCTCC CTTCGCTGAC
    ACCTTCAAGA AGCGCGTCAG CAAGAACACT TTCTTCGTGC TGGTGCGAGT GGTGGATGAG
    CTCTGCCTAG TGGAGTTGAC AGAAGAGATC TTGAAGCTGC TCATGGAACT GGTCTTTCGC
    TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC CGCGTGCTCC GGAAGAACAT CCTGGACAAG
    GTGGACCAGA AGAAGCTGCT CAGGTGTGCC AACTCCGACC AGCCCCTGGC AGCCAGGGGG
    GTAGCTGCCA GGCCAGGGAC TTTGCATGAC TTTCACAGCC ATGAAATAGC CGAGCAGCTG
    ACACTGCTGG ATGCTGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCA
    AAAGAACAGA ATGAGGAGAA AAGCCCCAAC TTGACCCAGT TCACGGAGCA CTTCAACAAC
    ATGTCGTACT GGGTCCGGTC AATAATCATG TTACAAGAAA AAGCCCAGGA CAGGGAGCGA
    CTGCTACTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTTAACTCC
    TACCTGGCCA TCCTCTCGGC ACTGGACTCA GCTCCCATCC GCAGGCTAGA GTGGCAGAAG
    CAGACCTCGG AGGGCCTGGC CGAGTACTGT ACACTGATCG ACAGCTCGTC CTCCTTCCGC
    GCCTACCGGG CCGCCCTGTC CGAGGTGGAG CCGCCCTGCA TCCCATACCT GGGGCTTATC
    CTGCAGGACC TCACCTTTGT TCACCTGGGA AACCCAGACT ATATTGACGG GAAGGTGAAT
    TTTTCCAAAC GGTGGCAGCA GTTCAACATT CTGGACAGCA TGCGGTGCTT CCAACAAGCG
    CACTATGATA TCCGAAGAAA CGATGACATC ATAAACTTCT TCAATGACTT CAGTGACCAC
    CTGGCGGAGG AGGCTCTGTG GGAACTCTCT CTGAAAATCA AACCTAGGAA CATAACAAGG
    AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12673
    Clone ID Related Accession (Same CDS sequence) XM_005625218.2 , XM_005625218.3
    Accession Version XM_005625218.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGGAGTCAC CAGCTATGAA AGATGGACAT
    CCCAGAGACC CCTCATCGGT CAGCAGCACA CCTGGGAAGG AGAGCAGAGA CAGCGACAGG
    GCCTCAAGGT CACCAGATGG TTCAGAGTTG GCTCGGTTGG AGGAGGAAGT GGATGAGCTG
    TCCCTCATTG ACCACAATGA AATTATGGCA AGGCTGACAC TCAAGCAGGA GGGTGATGAT
    GGGCCAGACG TCCGTGGAGG GTCTGGAGAT ATCCTACTGG TGCACGCTAC TGAGACAGAC
    AGGAAAGATT TGGTGTTGTA CTGTGAGGCC TTCTTGACCA CCTACAGGAC ATTCATCACC
    CCAGAGGAGC TCATCAAGAA GCTGCAGTAC AGGTACGAGA AGTTCTCTCC CTTCGCTGAC
    ACCTTCAAGA AGCGCGTCAG CAAGAACACT TTCTTCGTGC TGGTGCGAGT GGTGGATGAG
    CTCTGCCTAG TGGAGTTGAC AGAAGAGATC TTGAAGCTGC TCATGGAACT GGTCTTTCGC
    TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC CGCGTGCTCC GGAAGAACAT CCTGGACAAG
    GTGGACCAGA AGAAGCTGCT CAGGTGTGCC AACTCCGACC AGCCCCTGGC AGCCAGGGGG
    GTAGCTGCCA GGCCAGGGAC TTTGCATGAC TTTCACAGCC ATGAAATAGC CGAGCAGCTG
    ACACTGCTGG ATGCTGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCA
    AAAGAACAGA ATGAGGAGAA AAGCCCCAAC TTGACCCAGT TCACGGAGCA CTTCAACAAC
    ATGTCGTACT GGGTCCGGTC AATAATCATG TTACAAGAAA AAGCCCAGGA CAGGGAGCGA
    CTGCTACTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTTAACTCC
    TACCTGGCCA TCCTCTCGGC ACTGGACTCA GCTCCCATCC GCAGGCTAGA GTGGCAGAAG
    CAGACCTCGG AGGGCCTGGC CGAGTACTGT ACACTGATCG ACAGCTCGTC CTCCTTCCGC
    GCCTACCGGG CCGCCCTGTC CGAGGTGGAG CCGCCCTGCA TCCCATACCT GGGGCTTATC
    CTGCAGGACC TCACCTTTGT TCACCTGGGA AACCCAGACT ATATTGACGG GAAGGTGAAT
    TTTTCCAAAC GGTGGCAGCA GTTCAACATT CTGGACAGCA TGCGGTGCTT CCAACAAGCG
    CACTATGATA TCCGAAGAAA CGATGACATC ATAAACTTCT TCAATGACTT CAGTGACCAC
    CTGGCGGAGG AGGCTCTGTG GGAACTCTCT CTGAAAATCA AACCTAGGAA CATAACAAGG
    AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625275.1
    CDS352..3801
    Translation

    Target ORF information:

    RefSeq Version XM_005625218.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625218.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGCAGGCC
    TCCTATGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC
    TTCACCCCCG AGGACGGCAG TGCCACTCAG GGCATCAGTG TGTCCGTCTC TAACTCCTTT
    CTCAGCCGGC ATGGCAACTT GCCTGTGCCC TCGGAGTCAC CAGCTATGAA AGATGGACAT
    CCCAGAGACC CCTCATCGGT CAGCAGCACA CCTGGGAAGG AGAGCAGAGA CAGCGACAGG
    GCCTCAAGGT CACCAGATGG TTCAGAGTTG GCTCGGTTGG AGGAGGAAGT GGATGAGCTG
    TCCCTCATTG ACCACAATGA AATTATGGCA AGGCTGACAC TCAAGCAGGA GGGTGATGAT
    GGGCCAGACG TCCGTGGAGG GTCTGGAGAT ATCCTACTGG TGCACGCTAC TGAGACAGAC
    AGGAAAGATT TGGTGTTGTA CTGTGAGGCC TTCTTGACCA CCTACAGGAC ATTCATCACC
    CCAGAGGAGC TCATCAAGAA GCTGCAGTAC AGGTACGAGA AGTTCTCTCC CTTCGCTGAC
    ACCTTCAAGA AGCGCGTCAG CAAGAACACT TTCTTCGTGC TGGTGCGAGT GGTGGATGAG
    CTCTGCCTAG TGGAGTTGAC AGAAGAGATC TTGAAGCTGC TCATGGAACT GGTCTTTCGC
    TTGGTGTGCA ACGGGGAGCT CAGCCTGGCC CGCGTGCTCC GGAAGAACAT CCTGGACAAG
    GTGGACCAGA AGAAGCTGCT CAGGTGTGCC AACTCCGACC AGCCCCTGGC AGCCAGGGGG
    GTAGCTGCCA GGCCAGGGAC TTTGCATGAC TTTCACAGCC ATGAAATAGC CGAGCAGCTG
    ACACTGCTGG ATGCTGAGCT CTTTTATAAA ATAGAGATTC CTGAGGTTTT GCTTTGGGCA
    AAAGAACAGA ATGAGGAGAA AAGCCCCAAC TTGACCCAGT TCACGGAGCA CTTCAACAAC
    ATGTCGTACT GGGTCCGGTC AATAATCATG TTACAAGAAA AAGCCCAGGA CAGGGAGCGA
    CTGCTACTGA AGTTCATCAA GATCATGAAG CACTTACGGA AGCTGAACAA CTTTAACTCC
    TACCTGGCCA TCCTCTCGGC ACTGGACTCA GCTCCCATCC GCAGGCTAGA GTGGCAGAAG
    CAGACCTCGG AGGGCCTGGC CGAGTACTGT ACACTGATCG ACAGCTCGTC CTCCTTCCGC
    GCCTACCGGG CCGCCCTGTC CGAGGTGGAG CCGCCCTGCA TCCCATACCT GGGGCTTATC
    CTGCAGGACC TCACCTTTGT TCACCTGGGA AACCCAGACT ATATTGACGG GAAGGTGAAT
    TTTTCCAAAC GGTGGCAGCA GTTCAACATT CTGGACAGCA TGCGGTGCTT CCAACAAGCG
    CACTATGATA TCCGAAGAAA CGATGACATC ATAAACTTCT TCAATGACTT CAGTGACCAC
    CTGGCGGAGG AGGCTCTGTG GGAACTCTCT CTGAAAATCA AACCTAGGAA CATAACAAGG
    AGAAAAACAG ACCGAGAGGA GAAGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12674
    Clone ID Related Accession (Same CDS sequence) XM_005625219.3 , XM_005625219.2
    Accession Version XM_005625219.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rap guanine nucleotide exchange factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGAGTCA
    CCAGCTATGA AAGATGGACA TCCCAGAGAC CCCTCATCGG TCAGCAGCAC ACCTGGGAAG
    GAGAGCAGAG ACAGCGACAG GGCCTCAAGG TCACCAGATG GTTCAGAGTT GGCTCGGTTG
    GAGGAGGAAG TGGATGAGCT GTCCCTCATT GACCACAATG AAATTATGGC AAGGCTGACA
    CTCAAGCAGG AGGGTGATGA TGGGCCAGAC GTCCGTGGAG GGTCTGGAGA TATCCTACTG
    GTGCACGCTA CTGAGACAGA CAGGAAAGAT TTGGTGTTGT ACTGTGAGGC CTTCTTGACC
    ACCTACAGGA CATTCATCAC CCCAGAGGAG CTCATCAAGA AGCTGCAGTA CAGGTACGAG
    AAGTTCTCTC CCTTCGCTGA CACCTTCAAG AAGCGCGTCA GCAAGAACAC TTTCTTCGTG
    CTGGTGCGAG TGGTGGATGA GCTCTGCCTA GTGGAGTTGA CAGAAGAGAT CTTGAAGCTG
    CTCATGGAAC TGGTCTTTCG CTTGGTGTGC AACGGGGAGC TCAGCCTGGC CCGCGTGCTC
    CGGAAGAACA TCCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CAACTCCGAC
    CAGCCCCTGG CAGCCAGGGG GGTAGCTGCC AGGCCAGGGA CTTTGCATGA CTTTCACAGC
    CATGAAATAG CCGAGCAGCT GACACTGCTG GATGCTGAGC TCTTTTATAA AATAGAGATT
    CCTGAGGTTT TGCTTTGGGC AAAAGAACAG AATGAGGAGA AAAGCCCCAA CTTGACCCAG
    TTCACGGAGC ACTTCAACAA CATGTCGTAC TGGGTCCGGT CAATAATCAT GTTACAAGAA
    AAAGCCCAGG ACAGGGAGCG ACTGCTACTG AAGTTCATCA AGATCATGAA GCACTTACGG
    AAGCTGAACA ACTTTAACTC CTACCTGGCC ATCCTCTCGG CACTGGACTC AGCTCCCATC
    CGCAGGCTAG AGTGGCAGAA GCAGACCTCG GAGGGCCTGG CCGAGTACTG TACACTGATC
    GACAGCTCGT CCTCCTTCCG CGCCTACCGG GCCGCCCTGT CCGAGGTGGA GCCGCCCTGC
    ATCCCATACC TGGGGCTTAT CCTGCAGGAC CTCACCTTTG TTCACCTGGG AAACCCAGAC
    TATATTGACG GGAAGGTGAA TTTTTCCAAA CGGTGGCAGC AGTTCAACAT TCTGGACAGC
    ATGCGGTGCT TCCAACAAGC GCACTATGAT ATCCGAAGAA ACGATGACAT CATAAACTTC
    TTCAATGACT TCAGTGACCA CCTGGCGGAG GAGGCTCTGT GGGAACTCTC TCTGAAAATC
    AAACCTAGGA ACATAACAAG GAGAAAAACA GACCGAGAGG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625276.1
    CDS351..3641
    Translation

    Target ORF information:

    RefSeq Version XM_005625219.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625219.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGAGTCA
    CCAGCTATGA AAGATGGACA TCCCAGAGAC CCCTCATCGG TCAGCAGCAC ACCTGGGAAG
    GAGAGCAGAG ACAGCGACAG GGCCTCAAGG TCACCAGATG GTTCAGAGTT GGCTCGGTTG
    GAGGAGGAAG TGGATGAGCT GTCCCTCATT GACCACAATG AAATTATGGC AAGGCTGACA
    CTCAAGCAGG AGGGTGATGA TGGGCCAGAC GTCCGTGGAG GGTCTGGAGA TATCCTACTG
    GTGCACGCTA CTGAGACAGA CAGGAAAGAT TTGGTGTTGT ACTGTGAGGC CTTCTTGACC
    ACCTACAGGA CATTCATCAC CCCAGAGGAG CTCATCAAGA AGCTGCAGTA CAGGTACGAG
    AAGTTCTCTC CCTTCGCTGA CACCTTCAAG AAGCGCGTCA GCAAGAACAC TTTCTTCGTG
    CTGGTGCGAG TGGTGGATGA GCTCTGCCTA GTGGAGTTGA CAGAAGAGAT CTTGAAGCTG
    CTCATGGAAC TGGTCTTTCG CTTGGTGTGC AACGGGGAGC TCAGCCTGGC CCGCGTGCTC
    CGGAAGAACA TCCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CAACTCCGAC
    CAGCCCCTGG CAGCCAGGGG GGTAGCTGCC AGGCCAGGGA CTTTGCATGA CTTTCACAGC
    CATGAAATAG CCGAGCAGCT GACACTGCTG GATGCTGAGC TCTTTTATAA AATAGAGATT
    CCTGAGGTTT TGCTTTGGGC AAAAGAACAG AATGAGGAGA AAAGCCCCAA CTTGACCCAG
    TTCACGGAGC ACTTCAACAA CATGTCGTAC TGGGTCCGGT CAATAATCAT GTTACAAGAA
    AAAGCCCAGG ACAGGGAGCG ACTGCTACTG AAGTTCATCA AGATCATGAA GCACTTACGG
    AAGCTGAACA ACTTTAACTC CTACCTGGCC ATCCTCTCGG CACTGGACTC AGCTCCCATC
    CGCAGGCTAG AGTGGCAGAA GCAGACCTCG GAGGGCCTGG CCGAGTACTG TACACTGATC
    GACAGCTCGT CCTCCTTCCG CGCCTACCGG GCCGCCCTGT CCGAGGTGGA GCCGCCCTGC
    ATCCCATACC TGGGGCTTAT CCTGCAGGAC CTCACCTTTG TTCACCTGGG AAACCCAGAC
    TATATTGACG GGAAGGTGAA TTTTTCCAAA CGGTGGCAGC AGTTCAACAT TCTGGACAGC
    ATGCGGTGCT TCCAACAAGC GCACTATGAT ATCCGAAGAA ACGATGACAT CATAAACTTC
    TTCAATGACT TCAGTGACCA CCTGGCGGAG GAGGCTCTGT GGGAACTCTC TCTGAAAATC
    AAACCTAGGA ACATAACAAG GAGAAAAACA GACCGAGAGG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12674
    Clone ID Related Accession (Same CDS sequence) XM_005625219.3 , XM_005625219.2
    Accession Version XM_005625219.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rap guanine nucleotide exchange factor 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGAGTCA
    CCAGCTATGA AAGATGGACA TCCCAGAGAC CCCTCATCGG TCAGCAGCAC ACCTGGGAAG
    GAGAGCAGAG ACAGCGACAG GGCCTCAAGG TCACCAGATG GTTCAGAGTT GGCTCGGTTG
    GAGGAGGAAG TGGATGAGCT GTCCCTCATT GACCACAATG AAATTATGGC AAGGCTGACA
    CTCAAGCAGG AGGGTGATGA TGGGCCAGAC GTCCGTGGAG GGTCTGGAGA TATCCTACTG
    GTGCACGCTA CTGAGACAGA CAGGAAAGAT TTGGTGTTGT ACTGTGAGGC CTTCTTGACC
    ACCTACAGGA CATTCATCAC CCCAGAGGAG CTCATCAAGA AGCTGCAGTA CAGGTACGAG
    AAGTTCTCTC CCTTCGCTGA CACCTTCAAG AAGCGCGTCA GCAAGAACAC TTTCTTCGTG
    CTGGTGCGAG TGGTGGATGA GCTCTGCCTA GTGGAGTTGA CAGAAGAGAT CTTGAAGCTG
    CTCATGGAAC TGGTCTTTCG CTTGGTGTGC AACGGGGAGC TCAGCCTGGC CCGCGTGCTC
    CGGAAGAACA TCCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CAACTCCGAC
    CAGCCCCTGG CAGCCAGGGG GGTAGCTGCC AGGCCAGGGA CTTTGCATGA CTTTCACAGC
    CATGAAATAG CCGAGCAGCT GACACTGCTG GATGCTGAGC TCTTTTATAA AATAGAGATT
    CCTGAGGTTT TGCTTTGGGC AAAAGAACAG AATGAGGAGA AAAGCCCCAA CTTGACCCAG
    TTCACGGAGC ACTTCAACAA CATGTCGTAC TGGGTCCGGT CAATAATCAT GTTACAAGAA
    AAAGCCCAGG ACAGGGAGCG ACTGCTACTG AAGTTCATCA AGATCATGAA GCACTTACGG
    AAGCTGAACA ACTTTAACTC CTACCTGGCC ATCCTCTCGG CACTGGACTC AGCTCCCATC
    CGCAGGCTAG AGTGGCAGAA GCAGACCTCG GAGGGCCTGG CCGAGTACTG TACACTGATC
    GACAGCTCGT CCTCCTTCCG CGCCTACCGG GCCGCCCTGT CCGAGGTGGA GCCGCCCTGC
    ATCCCATACC TGGGGCTTAT CCTGCAGGAC CTCACCTTTG TTCACCTGGG AAACCCAGAC
    TATATTGACG GGAAGGTGAA TTTTTCCAAA CGGTGGCAGC AGTTCAACAT TCTGGACAGC
    ATGCGGTGCT TCCAACAAGC GCACTATGAT ATCCGAAGAA ACGATGACAT CATAAACTTC
    TTCAATGACT TCAGTGACCA CCTGGCGGAG GAGGCTCTGT GGGAACTCTC TCTGAAAATC
    AAACCTAGGA ACATAACAAG GAGAAAAACA GACCGAGAGG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625276.1
    CDS354..3644
    Translation

    Target ORF information:

    RefSeq Version XM_005625219.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625219.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAATG CTGCTGAGAA ACAGAAGCCC CGGAAGCGAA GCCGTCTGTG CCCCTGGAAG 
    CAAGATTCTC AGCGTTCTCA CCTCTCTTCC TTCACCATGA AGCTGAAGGA CAAATTCCAC
    TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAAGT TTCTGTGAAA
    AATCCGGAGA AGCCTGTGAG CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT
    CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC
    AGGTCTCAGA GGCAGAAGAA CCTGAGCTGG CTGGAAGAGA AGGAGAAGGA AGTTGTTAGT
    GCCTTGCGCT ACTTTAAGAC TATCGTGGAC AAGATGGCTA TTGATAAGAA GGTACTGGAG
    ATGCTGCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAGCGAT
    CCTCGAATTC AACACAGCTC AGCCCTCTCC TCCTGCTATA GCCGAGTGTA CCAAAGTCTT
    GCCAACCTCA TCCGTTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGATAAA
    GAGACGGTGA CAACGGTGAA GGGGGTCATC AAGGCGGTAC TGGATGGAGT GAAGGAGCTG
    GTCAGACTTA CCATCGAGAA ACAGGGGCAT CCATCTCCAA CCAGCCCAGT GAAACCCAGT
    TCCCCAGCCT GCAAACCGGA CGGCCAGTCT GAGCTCCCCC TGACAGATCG CGAGGTGGAG
    ATTCTCAACA AGACAACTGG CATGTCCCAG TCGGCCGAGC TACTCCCAGA CTCAACAGAT
    GAGGAGGTTG CGCCCCCTAA GCCCCCCTTA CCTGGCATTC GGGTGGTTGA TAACAGTCCT
    CCCCCAGCAT TGCCACCGAA GAAAAGACAG TCAGCTCCAT CCCCGACCCG AGTGGCTGTT
    GTTGCCCCCA TGAGTCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT TAATAGGCAG
    GATTTTGATG TGGACTGTTA CACACAGAGG CGACTCTCAG GAGGCAGCCA CTCATACGGC
    GGAGAGTCGC CCCGTCTGTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGATGAG
    CAGCTGTCCT CTCTGGACAG GGACAGCGGC CAGTGCTCCC GGAACACAAG CTGTGAAACA
    CTAGATCATT ATGATCCTGA CTACGAATTC CTTCAGCAAG ACCTCTCTAA TGCAGACCAG
    ATACCTCAGC AAGTGGCCTG TAACCTTAGC CCATTGCCAG AGTCTTTGGG AGAGTCTGGG
    TCTCCATTTC TTGGCCATCC TTTCCAGTTG CCTCCAGCGC CTGGCAGCTG TCCCCAGCCA
    GAGGGGCCCT TGGCCCCAGG ACAGCAGATA GACATGCCAC CTGCTCTCCC TGAGAAGAAG
    CGCAGGAGTG CGGCCTCCCA GACCACGGAC AGCTCTGGCT GCAGGATGTC CTACGAGCGG
    CACCCCTCAC AATATGACAA TATCTCGGAA GATGACCTGC AGAACCCAGC CTCGGTCCCG
    TCCATCCCCT TCACGCCCTT TGCTGCTATT CTCCCCTTTC AGCAAGGAGG GTCCTCAGCC
    TCTGTCGAAT TTGTGGGTGA TTTCACTGTT TCTGAATCAA CAACGGGTGA CCCAGAAAAA
    CCACCTCCTC TACCAGAGAA GAAAAACAAA CACATGCTGG CCTACATGCA GCTGCTGGAG
    GACTACTCGG AGCCACAGCC GTCCATGTTC TACCAGACGC CACAGAACGA GCACATCTAC
    CAGCAGAAGA ACAAGCTCCT CATGGAGGTG TACGGCTTCA ACGACTCATT CAGCACCGAA
    GCCCCGCAGG AGCTGGCACC GCCCCCCGCC CTGCCTCCCA AGCAGCGGCA GCTGGAGTCA
    CCAGCTATGA AAGATGGACA TCCCAGAGAC CCCTCATCGG TCAGCAGCAC ACCTGGGAAG
    GAGAGCAGAG ACAGCGACAG GGCCTCAAGG TCACCAGATG GTTCAGAGTT GGCTCGGTTG
    GAGGAGGAAG TGGATGAGCT GTCCCTCATT GACCACAATG AAATTATGGC AAGGCTGACA
    CTCAAGCAGG AGGGTGATGA TGGGCCAGAC GTCCGTGGAG GGTCTGGAGA TATCCTACTG
    GTGCACGCTA CTGAGACAGA CAGGAAAGAT TTGGTGTTGT ACTGTGAGGC CTTCTTGACC
    ACCTACAGGA CATTCATCAC CCCAGAGGAG CTCATCAAGA AGCTGCAGTA CAGGTACGAG
    AAGTTCTCTC CCTTCGCTGA CACCTTCAAG AAGCGCGTCA GCAAGAACAC TTTCTTCGTG
    CTGGTGCGAG TGGTGGATGA GCTCTGCCTA GTGGAGTTGA CAGAAGAGAT CTTGAAGCTG
    CTCATGGAAC TGGTCTTTCG CTTGGTGTGC AACGGGGAGC TCAGCCTGGC CCGCGTGCTC
    CGGAAGAACA TCCTGGACAA GGTGGACCAG AAGAAGCTGC TCAGGTGTGC CAACTCCGAC
    CAGCCCCTGG CAGCCAGGGG GGTAGCTGCC AGGCCAGGGA CTTTGCATGA CTTTCACAGC
    CATGAAATAG CCGAGCAGCT GACACTGCTG GATGCTGAGC TCTTTTATAA AATAGAGATT
    CCTGAGGTTT TGCTTTGGGC AAAAGAACAG AATGAGGAGA AAAGCCCCAA CTTGACCCAG
    TTCACGGAGC ACTTCAACAA CATGTCGTAC TGGGTCCGGT CAATAATCAT GTTACAAGAA
    AAAGCCCAGG ACAGGGAGCG ACTGCTACTG AAGTTCATCA AGATCATGAA GCACTTACGG
    AAGCTGAACA ACTTTAACTC CTACCTGGCC ATCCTCTCGG CACTGGACTC AGCTCCCATC
    CGCAGGCTAG AGTGGCAGAA GCAGACCTCG GAGGGCCTGG CCGAGTACTG TACACTGATC
    GACAGCTCGT CCTCCTTCCG CGCCTACCGG GCCGCCCTGT CCGAGGTGGA GCCGCCCTGC
    ATCCCATACC TGGGGCTTAT CCTGCAGGAC CTCACCTTTG TTCACCTGGG AAACCCAGAC
    TATATTGACG GGAAGGTGAA TTTTTCCAAA CGGTGGCAGC AGTTCAACAT TCTGGACAGC
    ATGCGGTGCT TCCAACAAGC GCACTATGAT ATCCGAAGAA ACGATGACAT CATAAACTTC
    TTCAATGACT TCAGTGACCA CCTGGCGGAG GAGGCTCTGT GGGAACTCTC TCTGAAAATC
    AAACCTAGGA ACATAACAAG GAGAAAAACA GACCGAGAGG AGAAGACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.