rapgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rapgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa63734 XM_012968551.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa63735 XM_012968552.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa63736 XM_031890514.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63737 XM_012968553.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa22617 NM_001045665.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa28737 XM_012968551.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa28738 XM_012968552.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa28739 XM_012968553.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa63734
    Clone ID Related Accession (Same CDS sequence) XM_012968551.3
    Accession Version XM_012968551.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC
    CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA
    CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC
    GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG
    GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG
    CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC
    TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC
    CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA
    TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC
    TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG
    AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC
    ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT
    TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT
    TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA
    ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT
    TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG
    CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG
    CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC
    GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC
    ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA
    CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG
    AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT
    TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC
    ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC
    CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824005.1
    CDS278..3637
    Translation

    Target ORF information:

    RefSeq Version XM_012968551.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968551.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC
    CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA
    CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC
    GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG
    GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG
    CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC
    TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC
    CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA
    TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC
    TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG
    AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC
    ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT
    TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT
    TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA
    ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT
    TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG
    CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG
    CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC
    GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC
    ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA
    CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG
    AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT
    TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC
    ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC
    CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63735
    Clone ID Related Accession (Same CDS sequence) XM_012968552.3
    Accession Version XM_012968552.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG
    CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT
    TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT
    GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG
    GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA
    GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG
    GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC
    AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC
    TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG
    TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG
    AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA
    GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT
    CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA
    GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG
    TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG
    CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC
    CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG
    ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC
    TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG
    CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC
    TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT
    TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG
    GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC
    AAAACAGAAA GGGACGAGAA AACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824006.1
    CDS278..3544
    Translation

    Target ORF information:

    RefSeq Version XM_012968552.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968552.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG
    CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT
    TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT
    GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG
    GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA
    GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG
    GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC
    AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC
    TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG
    TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG
    AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA
    GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT
    CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA
    GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG
    TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG
    CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC
    CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG
    ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC
    TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG
    CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC
    TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT
    TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG
    GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC
    AAAACAGAAA GGGACGAGAA AACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63736
    Clone ID Related Accession (Same CDS sequence) XM_031890514.1
    Accession Version XM_031890514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGTACAA ATCTGTTTTC CGCTCCTACT CCCAGGATTT TGCCCCTCAT
    AACCAAGCCC CACCACTTCT GGCCCCTGCC ATCTTCTACT CCTCCCGCTT CTCCCCCGTG
    CTCTCCGACT CCGCCTCTTC TCCCCCCGCC ATGCCCCAAG TACCCCTCCG CCCCCCCTTA
    CCTCCCTCTC AGGGTGGCAG CTCCTGCGGG ACCCCACCTA CCTGCCTTGC CTATGAGCCT
    TCTCTCTCCG GCACCTTGCA AACATCAGCG GATTCTCCTT TACCCAATGA AGGGCCTCAG
    CAGAACTGCA CCCTGCCCTG TCTGCCAGGC AAAGACAACC ACGAGGCTTC AGAGAGGCCT
    CCAAAGTCCC CCGATTGCCA CAGCGATGAG AATGGCGAGG GGGAAGAGGA AGAAGAGCTC
    GATGAACTCT CCCTTGTTGA CCATAACGAG ATTATGGCTA AGCTGACCCT GAAGCAAGAG
    GGTGATGATG GACCGGACGT GCGGGGCGGC TCGGGTGACA TCTTGCTGGT CCATGCAACG
    GAAACCGATC GCAAAGATCT CGTCCTGTAC TTCGAAGCCT TCCTGACAAC ATACCGCACC
    TTTATTACCC CCGAGGAGCT GATTCAGAAG CTCCAGTACA GATATGAGAG GTTCTGTCAC
    TTTCAAGACA CTTTCAAGCA GCGCGTGAGC AAGAACACCT TCTTTGTGCT GGTGAGAGTG
    GTGGACGAGT TATGCTTGGT CGAGATGACA GATGAGATCC TGAAGCTGCT GATGGAACTC
    GTCTTCCGTC TGGTGTGTAA AGGGGAACTT AGCCTGGCTC GCATCTTGCG CAAGAACATT
    CTGGAAAAGG TGGAGAATAA GAGAATGCTG CACCACGCCA ATTCTGCACT TAAGCCCCTG
    GCTGCCAGAG GAGTAGCAGC AAGGCCAGGG ACGCTCCACG ATTTCCACAG TCTTGAAATT
    GCAGAGCAGC TGACTCTGCT GGATGCAGAA CTCTTCTATA AAATAGAGAT TCCTGAGGTT
    CTGCTTTGGG CAAAAGAGCA AAACGAGGAG AAGAGCCCCA ATTTAACACA GTTCACAGAG
    CACTTTAACA ACATGTCATA CTGGGTCCGA TCTATTATCA TGCTTCAGGA GAAAGCCCAG
    GACCGAGAGC GATTGCTGCT TAAATTCATT AAAATCATGA AGCACCTTCG GAAACTGAAC
    AACTTTAACT CCTACCTTGC CATCCTGTCC GCGCTGGACT CCGCCCCCAT TCGCAGGCTA
    GAATGGCAGA AGCAGACGTC AGAGGGCCTG GCGGAATACT GCACATTGAT TGACAGCTCG
    TCCTCCTTCA GAGCCTATAG GGCAGCCCTG GCTGAAGTTG AACCCCCCTG TATCCCCTAC
    TTAGGCCTGA TCCTGCAGGA TCTTACCTTT GTACATCTGG GGAACCCCGA CCACATTGAT
    GGGAAAGTCA ACTTCTCCAA GCGTTGGCAG CAGTTCAACA TTTTGGACAG TATGAGGCGA
    TTCCAGCAAG TACATTACGA GATCCGCCGC AACGACGAAA TCATCTCCTT CTTCAACGAC
    TTCAGCGACC ACCTGGCCGA GGAGGCTCTG TGGGAACTGT CCCTAAAGAT AAAACCTCGC
    AACATCACCC GGCGCAAAAC AGAAAGGGAC GAGAAAACTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746374.1
    CDS288..3809
    Translation

    Target ORF information:

    RefSeq Version XM_031890514.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGTACAA ATCTGTTTTC CGCTCCTACT CCCAGGATTT TGCCCCTCAT
    AACCAAGCCC CACCACTTCT GGCCCCTGCC ATCTTCTACT CCTCCCGCTT CTCCCCCGTG
    CTCTCCGACT CCGCCTCTTC TCCCCCCGCC ATGCCCCAAG TACCCCTCCG CCCCCCCTTA
    CCTCCCTCTC AGGGTGGCAG CTCCTGCGGG ACCCCACCTA CCTGCCTTGC CTATGAGCCT
    TCTCTCTCCG GCACCTTGCA AACATCAGCG GATTCTCCTT TACCCAATGA AGGGCCTCAG
    CAGAACTGCA CCCTGCCCTG TCTGCCAGGC AAAGACAACC ACGAGGCTTC AGAGAGGCCT
    CCAAAGTCCC CCGATTGCCA CAGCGATGAG AATGGCGAGG GGGAAGAGGA AGAAGAGCTC
    GATGAACTCT CCCTTGTTGA CCATAACGAG ATTATGGCTA AGCTGACCCT GAAGCAAGAG
    GGTGATGATG GACCGGACGT GCGGGGCGGC TCGGGTGACA TCTTGCTGGT CCATGCAACG
    GAAACCGATC GCAAAGATCT CGTCCTGTAC TTCGAAGCCT TCCTGACAAC ATACCGCACC
    TTTATTACCC CCGAGGAGCT GATTCAGAAG CTCCAGTACA GATATGAGAG GTTCTGTCAC
    TTTCAAGACA CTTTCAAGCA GCGCGTGAGC AAGAACACCT TCTTTGTGCT GGTGAGAGTG
    GTGGACGAGT TATGCTTGGT CGAGATGACA GATGAGATCC TGAAGCTGCT GATGGAACTC
    GTCTTCCGTC TGGTGTGTAA AGGGGAACTT AGCCTGGCTC GCATCTTGCG CAAGAACATT
    CTGGAAAAGG TGGAGAATAA GAGAATGCTG CACCACGCCA ATTCTGCACT TAAGCCCCTG
    GCTGCCAGAG GAGTAGCAGC AAGGCCAGGG ACGCTCCACG ATTTCCACAG TCTTGAAATT
    GCAGAGCAGC TGACTCTGCT GGATGCAGAA CTCTTCTATA AAATAGAGAT TCCTGAGGTT
    CTGCTTTGGG CAAAAGAGCA AAACGAGGAG AAGAGCCCCA ATTTAACACA GTTCACAGAG
    CACTTTAACA ACATGTCATA CTGGGTCCGA TCTATTATCA TGCTTCAGGA GAAAGCCCAG
    GACCGAGAGC GATTGCTGCT TAAATTCATT AAAATCATGA AGCACCTTCG GAAACTGAAC
    AACTTTAACT CCTACCTTGC CATCCTGTCC GCGCTGGACT CCGCCCCCAT TCGCAGGCTA
    GAATGGCAGA AGCAGACGTC AGAGGGCCTG GCGGAATACT GCACATTGAT TGACAGCTCG
    TCCTCCTTCA GAGCCTATAG GGCAGCCCTG GCTGAAGTTG AACCCCCCTG TATCCCCTAC
    TTAGGCCTGA TCCTGCAGGA TCTTACCTTT GTACATCTGG GGAACCCCGA CCACATTGAT
    GGGAAAGTCA ACTTCTCCAA GCGTTGGCAG CAGTTCAACA TTTTGGACAG TATGAGGCGA
    TTCCAGCAAG TACATTACGA GATCCGCCGC AACGACGAAA TCATCTCCTT CTTCAACGAC
    TTCAGCGACC ACCTGGCCGA GGAGGCTCTG TGGGAACTGT CCCTAAAGAT AAAACCTCGC
    AACATCACCC GGCGCAAAAC AGAAAGGGAC GAGAAAACTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63737
    Clone ID Related Accession (Same CDS sequence) XM_012968553.3
    Accession Version XM_012968553.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG
    GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC
    TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT
    CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC
    AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC
    CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG
    CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC
    GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG
    ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG
    CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC
    GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA
    GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG
    AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA
    AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG
    GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA
    AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC
    TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT
    AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC
    ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA
    GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG
    GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT
    CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG
    CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG
    ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG
    GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA
    GAAAGGGACG AGAAAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824007.1
    CDS278..3478
    Translation

    Target ORF information:

    RefSeq Version XM_012968553.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968553.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG
    GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC
    TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT
    CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC
    AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC
    CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG
    CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC
    GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG
    ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG
    CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC
    GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA
    GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG
    AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA
    AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG
    GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA
    AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC
    TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT
    AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC
    ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA
    GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG
    GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT
    CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG
    CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG
    ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG
    GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA
    GAAAGGGACG AGAAAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22617
    Clone ID Related Accession (Same CDS sequence) NM_001045665.1
    Accession Version NM_001045665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR942489.2. ##Evidence-Data-START## Transcript exon combination :: CR942489.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCGGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACC CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC TAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCCGAC GGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGGGG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATATACC AGCAGAAGAA CAAGCATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCGTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGTT
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGGATT CTCCTTTACC CAATGAAGGG
    CCTCAGCAGA ACTGCACCCT GCCCTGTCTG CCAGGCAAAG ACAACCACGA GGCATCAGAG
    AGGCCTCCAA AGTCCCCCGA TTGCCACAGC GATGAGAATG GCGAGGGGGA AGAGGAAGAA
    GAGCTCGATG AACTCTCCCT TGTTGACCAT AACGAGATTA TGGCTAAGCT GACCCTGAAG
    CAAGAGGGTG ATGATGGACC GGACGTGCGG GGCGGCTCCG GTGACATCTT GCTGGTCCAT
    GCAACGGAAA CCGATCGCAA AGATCTCGTC CTGTACTTCG AAGCCTTCCT GACAACATAC
    CGCACCTTTA TTACCCCCGA GGAGCTGATT CAGAAGCTCC AGTACAGATA TGAGAGGTTC
    TGTCACTTTC AAGACACTTT CAAGCAGCGC GTGAGCAAGA ACACCTTCTT CGTGCTGGTG
    AGAGTGGTGG ACGAGTTATG CTTGGTCGAG ATGACAGATG AGATCCTGAA GCTGCTGATG
    GAACTCGTCT TCCGTCTGGT GTGTAAAGGG GAACTTAGCC TGGCTCGCAT CTTGCGCAAG
    AACATTCTGG AAAAGGTGGA GAATAAGAGA ATGCTGCACC ACGCCAATTC TGCACTTAAG
    CCCCTGGCTG CCAGAGGAGT AGCAGCAAGG CCAGGGACGC TCCACGATTT CCACAGTCTT
    GAAATTGCAG AGCAGCTGAC TCTGCTGGAT GCAGAACTCT TCTATAAAAT AGAGATTCCT
    GAGGTTCTGC TTTGGGCAAA AGAGCAAAAC GAGGAGAAGA GCCCCAATTT AACACAGTTC
    ACAGAGCACT TTAACAACAT GTCATACTGG GTCCGATCCA TTATCATGCT TCAGGAGAAA
    GCCCAGGACC GAGAGCGATT GCTGCTTAAA TTCATTAAAA TCATGAAGCA CCTTCGGAAA
    CTGAACAACT TTAACTCCTA CCTTGCCATC CTGTCTGCGC TGGACTCCGC CCCCATTCGC
    AGGCTGGAAT GGCAGAAGCA GACGTCAGAG GGCCTGGCGG AATACTGCAC ATTGATTGAC
    AGCTCGTCCT CCTTCAGAGC CTATAGGGCA GCCCTGGCTG AGGTTGAACC CCCCTGTATC
    CCCTACTTAG GCCTGATCCT GCAGGATCTT ACCTTTGTAC ATCTGGGGAA CCCCGACCAC
    ATTGATGGGA AAGTCAACTT CTCCAAGCGT TGGCAGCAGT TCAACATTTT GGACAGTATG
    AGGCGATTCC AGCAAGTACA TTACGAGATC CGCCGCAACG ATGAAATCAT CTCCTTCTTC
    AACGACTTCA GCGACCACCT GGCCGAGGAG GCTCTGTGGG AACTGTCCCT AAAGATAAAA
    CCTCGCAACG TCACCCGGCG CAAAACAGAA AGGGACGAGA AAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039130.1
    CDS26..3133
    Translation

    Target ORF information:

    RefSeq Version NM_001045665.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCGGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTGGATACC CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC TAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCCGAC GGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGGGG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATATACC AGCAGAAGAA CAAGCATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCGTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGTT
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGGATT CTCCTTTACC CAATGAAGGG
    CCTCAGCAGA ACTGCACCCT GCCCTGTCTG CCAGGCAAAG ACAACCACGA GGCATCAGAG
    AGGCCTCCAA AGTCCCCCGA TTGCCACAGC GATGAGAATG GCGAGGGGGA AGAGGAAGAA
    GAGCTCGATG AACTCTCCCT TGTTGACCAT AACGAGATTA TGGCTAAGCT GACCCTGAAG
    CAAGAGGGTG ATGATGGACC GGACGTGCGG GGCGGCTCCG GTGACATCTT GCTGGTCCAT
    GCAACGGAAA CCGATCGCAA AGATCTCGTC CTGTACTTCG AAGCCTTCCT GACAACATAC
    CGCACCTTTA TTACCCCCGA GGAGCTGATT CAGAAGCTCC AGTACAGATA TGAGAGGTTC
    TGTCACTTTC AAGACACTTT CAAGCAGCGC GTGAGCAAGA ACACCTTCTT CGTGCTGGTG
    AGAGTGGTGG ACGAGTTATG CTTGGTCGAG ATGACAGATG AGATCCTGAA GCTGCTGATG
    GAACTCGTCT TCCGTCTGGT GTGTAAAGGG GAACTTAGCC TGGCTCGCAT CTTGCGCAAG
    AACATTCTGG AAAAGGTGGA GAATAAGAGA ATGCTGCACC ACGCCAATTC TGCACTTAAG
    CCCCTGGCTG CCAGAGGAGT AGCAGCAAGG CCAGGGACGC TCCACGATTT CCACAGTCTT
    GAAATTGCAG AGCAGCTGAC TCTGCTGGAT GCAGAACTCT TCTATAAAAT AGAGATTCCT
    GAGGTTCTGC TTTGGGCAAA AGAGCAAAAC GAGGAGAAGA GCCCCAATTT AACACAGTTC
    ACAGAGCACT TTAACAACAT GTCATACTGG GTCCGATCCA TTATCATGCT TCAGGAGAAA
    GCCCAGGACC GAGAGCGATT GCTGCTTAAA TTCATTAAAA TCATGAAGCA CCTTCGGAAA
    CTGAACAACT TTAACTCCTA CCTTGCCATC CTGTCTGCGC TGGACTCCGC CCCCATTCGC
    AGGCTGGAAT GGCAGAAGCA GACGTCAGAG GGCCTGGCGG AATACTGCAC ATTGATTGAC
    AGCTCGTCCT CCTTCAGAGC CTATAGGGCA GCCCTGGCTG AGGTTGAACC CCCCTGTATC
    CCCTACTTAG GCCTGATCCT GCAGGATCTT ACCTTTGTAC ATCTGGGGAA CCCCGACCAC
    ATTGATGGGA AAGTCAACTT CTCCAAGCGT TGGCAGCAGT TCAACATTTT GGACAGTATG
    AGGCGATTCC AGCAAGTACA TTACGAGATC CGCCGCAACG ATGAAATCAT CTCCTTCTTC
    AACGACTTCA GCGACCACCT GGCCGAGGAG GCTCTGTGGG AACTGTCCCT AAAGATAAAA
    CCTCGCAACG TCACCCGGCG CAAAACAGAA AGGGACGAGA AAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28737
    Clone ID Related Accession (Same CDS sequence) XM_012968551.2
    Accession Version XM_012968551.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC
    CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA
    CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC
    GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG
    GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG
    CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC
    TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC
    CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA
    TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC
    TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG
    AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC
    ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT
    TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT
    TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA
    ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT
    TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG
    CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG
    CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC
    GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC
    ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA
    CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG
    AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT
    TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC
    ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC
    CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824005.1
    CDS306..3665
    Translation

    Target ORF information:

    RefSeq Version XM_012968551.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC
    CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA
    CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC
    GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG
    GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG
    CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC
    TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC
    CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA
    TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC
    TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG
    AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC
    ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT
    TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT
    TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA
    ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT
    TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG
    CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG
    CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC
    GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC
    ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA
    CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG
    AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT
    TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC
    ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC
    CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28738
    Clone ID Related Accession (Same CDS sequence) XM_012968552.2
    Accession Version XM_012968552.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG
    CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT
    TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT
    GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG
    GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA
    GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG
    GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC
    AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC
    TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG
    TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG
    AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA
    GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT
    CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA
    GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG
    TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG
    CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC
    CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG
    ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC
    TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG
    CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC
    TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT
    TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG
    GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC
    AAAACAGAAA GGGACGAGAA AACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824006.1
    CDS304..3570
    Translation

    Target ORF information:

    RefSeq Version XM_012968552.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968552.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC
    TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC
    AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC
    TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG
    CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT
    TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT
    GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG
    GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA
    GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG
    GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC
    AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC
    TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG
    TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG
    AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA
    GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT
    CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA
    GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG
    TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG
    CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC
    CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG
    ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC
    TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG
    CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC
    TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT
    TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG
    GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC
    AAAACAGAAA GGGACGAGAA AACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28739
    Clone ID Related Accession (Same CDS sequence) XM_012968553.2
    Accession Version XM_012968553.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG
    GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC
    TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT
    CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC
    AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC
    CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG
    CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC
    GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG
    ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG
    CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC
    GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA
    GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG
    AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA
    AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG
    GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA
    AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC
    TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT
    AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC
    ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA
    GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG
    GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT
    CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG
    CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG
    ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG
    GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA
    GAAAGGGACG AGAAAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824007.1
    CDS303..3503
    Translation

    Target ORF information:

    RefSeq Version XM_012968553.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968553.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
    AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG
    CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG
    GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG
    ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA
    GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC
    TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG
    CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG
    GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA
    TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA
    TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG
    GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA
    GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT
    GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC
    AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG
    TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT
    ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG
    TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG
    CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC
    CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC
    GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA
    AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC
    TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT
    CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG
    GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG
    AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG
    TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC
    CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC
    CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG
    GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC
    TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT
    CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC
    AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC
    CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG
    CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC
    GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG
    ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG
    CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC
    GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA
    GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG
    AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA
    AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG
    GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA
    AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC
    TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT
    AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC
    ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA
    GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG
    GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT
    CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG
    CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG
    ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG
    GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA
    GAAAGGGACG AGAAAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.