RAPGEF1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015279742.1	XP_015135228.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_015279744.1	XP_015135230.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_015279745.1	XP_015135231.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_015279749.1	XP_015135235.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_015279745.2	XP_015135231.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_015279747.1	XP_015135233.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015279748.1	XP_015135234.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_015279750.1	XP_015135236.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_015279751.1	XP_015135237.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_015279749.2	XP_015135235.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_015279750.2	XP_015135236.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_015279751.2	XP_015135237.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_015279752.2	XP_015135238.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_015279746.1	XP_015135232.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_015279752.1	XP_015135238.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_015279741.2	XP_015135227.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_015279742.2	XP_015135228.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_015279748.2	XP_015135234.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_015279743.1	XP_015135229.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_015279743.2	XP_015135229.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_015279744.2	XP_015135230.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_015279747.2	XP_015135233.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_015279741.1	XP_015135227.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_015279746.2	XP_015135232.1	rap guanine nucleotide exchange factor 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa16235	XM_015279746.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa16239	XM_015279747.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa16240	XM_015279750.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa16241	XM_015279751.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa16242	XM_015279752.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa43663	XM_015279741.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43664	XM_015279742.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43665	XM_015279743.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43666	XM_015279744.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43667	XM_015279745.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43668	XM_015279748.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43669	XM_015279749.2 Latest version!	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OGa43667	XM_015279745.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43669	XM_015279749.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa16240	XM_015279750.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa16241	XM_015279751.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa16242	XM_015279752.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa43664	XM_015279742.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43668	XM_015279748.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43665	XM_015279743.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43666	XM_015279744.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa16239	XM_015279747.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa43663	XM_015279741.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa16235	XM_015279746.1	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

RAPGEF1 ( XM_015279746.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135232.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa16235
Clone ID Related Accession (Same CDS sequence)	XM_015279746.1 , XM_015279746.2
Accession Version	XM_015279746.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGAG TGAAAACGCC 
AGTGAGGAGG CTGGTGAGGG TGAATACGTC AATCTGTATT CCTCTGGCCA GAGCAACGGG 
GAACTCCCTC ATTCTGGAGG AGAATCTCCC TCTGTCAAAG ACGGCCATTC CAAAGACTCC 
ACTTCAAACA GCAGTGCCAT GGGGAAGGAA GGCAAAGATG GTGCTGAGAG GCAGCAGCAG 
TCGCCAGATG CTTTGGAATC GTCACAGTTG GAGGAAGAAG TGGATGAGCT CTCCCTTATT 
GACCACAGTG AAATTATGGC TAGGTTAACA CTGAAGCAAG AGGGAGATGA TGGGCCAGAT 
GTCCGCGGTG GATCTGGAGA TATTTTGTTG GTGCATGCAA CAGAGACCGA CAGGAAAGAT 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACT ACATACAGGA CATTTATTAC CCCTGAAGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAGTTTTGCC ACTTCCCAGA CACGTTTAAG 
AAGCGAGTCA GTAAGAACAC CTTCTTCGTG CTGGTGAGAG TGGTGGATGA GCTGTGCTTA 
GTGGAGCTGA CGGAGGAGAT TCTCAAGCTG CTGATGGACC TGGTGTTCCG GCTGGTCTGC 
AATGGGGAGC TGAGCCTCGC CAGAGTGCTG CGCAAAAACA TCCTGGACAA AGTGGATCAG 
AAGAAGATGT TGAGATACGC CAACTCCATC AAACCTCTCG CAGCCCGCGG GGTGGCAGCC 
AGGCCGGGCA CCTTGCATGA TTTCCACAGT CATGAAATAG CCGAGCAGCT GACACTCTTA 
GATGCTGAAC TCTTCTATAA AATCGAGATA CCAGAAGTTT TGCTTTGGGC GAAAGAGCAA 
AATGAAGAGA AGAGCCCCAA CCTGACGCAG TTCACAGAGC ACTTTAATAA CATGTCCTAC 
TGGGTCCGTT CCATAATAAT GCTTCAAGAG AAAGCCCAAG ACCGAGAGAG GCTGCTGCTT 
AAGTTCATAA AGATTATGAA GCACTTACGA AAGCTGAATA ATTTTAATTC CTATCTGGCC 
ATTCTTTCTG CACTGGATTC TGCACCCATC CGAAGGCTGG AGTGGCAGAA ACAAACCTCA 
GAGGGCCTGG CTGAGTACTG CACACTGATC GACAGCTCCT CGTCCTTCCG CGCCTACCGT 
GCAGCCCTGG CTGATGTGGA GCCTCCCTGC ATCCCATACC TAGGCCTAAT TCTGCAGGAC 
CTGACCTTTG TTCATCTGGG AAACCCAGAC TACATCGACA GCAAGGTGAA CTTCTCCAAG 
CGCTGGCAGC AGTTTAACAT CCTGGACAGC ATGAGGTGCT TCCAACAGGT ACATTACGAC 
ATCAAGAGAA ACGACGACAT CGTGTCGTTC TTCAACGACT TCAGCGACCA TCTGGCGGAG 
GAGGCCCTGT GGGAACTGTC CCTCAAAATC AAACCTCGGA ACATTACAAG GCGGAAAACA 
GACCGGGAAG AGAAAACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135232.1
CDS	228..3908
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279746.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279746.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGAG TGAAAACGCC 
AGTGAGGAGG CTGGTGAGGG TGAATACGTC AATCTGTATT CCTCTGGCCA GAGCAACGGG 
GAACTCCCTC ATTCTGGAGG AGAATCTCCC TCTGTCAAAG ACGGCCATTC CAAAGACTCC 
ACTTCAAACA GCAGTGCCAT GGGGAAGGAA GGCAAAGATG GTGCTGAGAG GCAGCAGCAG 
TCGCCAGATG CTTTGGAATC GTCACAGTTG GAGGAAGAAG TGGATGAGCT CTCCCTTATT 
GACCACAGTG AAATTATGGC TAGGTTAACA CTGAAGCAAG AGGGAGATGA TGGGCCAGAT 
GTCCGCGGTG GATCTGGAGA TATTTTGTTG GTGCATGCAA CAGAGACCGA CAGGAAAGAT 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACT ACATACAGGA CATTTATTAC CCCTGAAGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAGTTTTGCC ACTTCCCAGA CACGTTTAAG 
AAGCGAGTCA GTAAGAACAC CTTCTTCGTG CTGGTGAGAG TGGTGGATGA GCTGTGCTTA 
GTGGAGCTGA CGGAGGAGAT TCTCAAGCTG CTGATGGACC TGGTGTTCCG GCTGGTCTGC 
AATGGGGAGC TGAGCCTCGC CAGAGTGCTG CGCAAAAACA TCCTGGACAA AGTGGATCAG 
AAGAAGATGT TGAGATACGC CAACTCCATC AAACCTCTCG CAGCCCGCGG GGTGGCAGCC 
AGGCCGGGCA CCTTGCATGA TTTCCACAGT CATGAAATAG CCGAGCAGCT GACACTCTTA 
GATGCTGAAC TCTTCTATAA AATCGAGATA CCAGAAGTTT TGCTTTGGGC GAAAGAGCAA 
AATGAAGAGA AGAGCCCCAA CCTGACGCAG TTCACAGAGC ACTTTAATAA CATGTCCTAC 
TGGGTCCGTT CCATAATAAT GCTTCAAGAG AAAGCCCAAG ACCGAGAGAG GCTGCTGCTT 
AAGTTCATAA AGATTATGAA GCACTTACGA AAGCTGAATA ATTTTAATTC CTATCTGGCC 
ATTCTTTCTG CACTGGATTC TGCACCCATC CGAAGGCTGG AGTGGCAGAA ACAAACCTCA 
GAGGGCCTGG CTGAGTACTG CACACTGATC GACAGCTCCT CGTCCTTCCG CGCCTACCGT 
GCAGCCCTGG CTGATGTGGA GCCTCCCTGC ATCCCATACC TAGGCCTAAT TCTGCAGGAC 
CTGACCTTTG TTCATCTGGG AAACCCAGAC TACATCGACA GCAAGGTGAA CTTCTCCAAG 
CGCTGGCAGC AGTTTAACAT CCTGGACAGC ATGAGGTGCT TCCAACAGGT ACATTACGAC 
ATCAAGAGAA ACGACGACAT CGTGTCGTTC TTCAACGACT TCAGCGACCA TCTGGCGGAG 
GAGGCCCTGT GGGAACTGTC CCTCAAAATC AAACCTCGGA ACATTACAAG GCGGAAAACA 
GACCGGGAAG AGAAAACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279746.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135232.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa16235
Clone ID Related Accession (Same CDS sequence)	XM_015279746.1 , XM_015279746.2
Accession Version	XM_015279746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGAG TGAAAACGCC 
AGTGAGGAGG CTGGTGAGGG TGAATACGTC AATCTGTATT CCTCTGGCCA GAGCAACGGG 
GAACTCCCTC ATTCTGGAGG AGAATCTCCC TCTGTCAAAG ACGGCCATTC CAAAGACTCC 
ACTTCAAACA GCAGTGCCAT GGGGAAGGAA GGCAAAGATG GTGCTGAGAG GCAGCAGCAG 
TCGCCAGATG CTTTGGAATC GTCACAGTTG GAGGAAGAAG TGGATGAGCT CTCCCTTATT 
GACCACAGTG AAATTATGGC TAGGTTAACA CTGAAGCAAG AGGGAGATGA TGGGCCAGAT 
GTCCGCGGTG GATCTGGAGA TATTTTGTTG GTGCATGCAA CAGAGACCGA CAGGAAAGAT 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACT ACATACAGGA CATTTATTAC CCCTGAAGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAGTTTTGCC ACTTCCCAGA CACGTTTAAG 
AAGCGAGTCA GTAAGAACAC CTTCTTCGTG CTGGTGAGAG TGGTGGATGA GCTGTGCTTA 
GTGGAGCTGA CGGAGGAGAT TCTCAAGCTG CTGATGGACC TGGTGTTCCG GCTGGTCTGC 
AATGGGGAGC TGAGCCTCGC CAGAGTGCTG CGCAAAAACA TCCTGGACAA AGTGGATCAG 
AAGAAGATGT TGAGATACGC CAACTCCATC AAACCTCTCG CAGCCCGCGG GGTGGCAGCC 
AGGCCGGGCA CCTTGCATGA TTTCCACAGT CATGAAATAG CCGAGCAGCT GACACTCTTA 
GATGCTGAAC TCTTCTATAA AATCGAGATA CCAGAAGTTT TGCTTTGGGC GAAAGAGCAA 
AATGAAGAGA AGAGCCCCAA CCTGACGCAG TTCACAGAGC ACTTTAATAA CATGTCCTAC 
TGGGTCCGTT CCATAATAAT GCTTCAAGAG AAAGCCCAAG ACCGAGAGAG GCTGCTGCTT 
AAGTTCATAA AGATTATGAA GCACTTACGA AAGCTGAATA ATTTTAATTC CTATCTGGCC 
ATTCTTTCTG CACTGGATTC TGCACCCATC CGAAGGCTGG AGTGGCAGAA ACAAACCTCA 
GAGGGCCTGG CTGAGTACTG CACACTGATC GACAGCTCCT CGTCCTTCCG CGCCTACCGT 
GCAGCCCTGG CTGATGTGGA GCCTCCCTGC ATCCCATACC TAGGCCTAAT TCTGCAGGAC 
CTGACCTTTG TTCATCTGGG AAACCCAGAC TACATCGACA GCAAGGTGAA CTTCTCCAAG 
CGCTGGCAGC AGTTTAACAT CCTGGACAGC ATGAGGTGCT TCCAACAGGT ACATTACGAC 
ATCAAGAGAA ACGACGACAT CGTGTCGTTC TTCAACGACT TCAGCGACCA TCTGGCGGAG 
GAGGCCCTGT GGGAACTGTC CCTCAAAATC AAACCTCGGA ACATTACAAG GCGGAAAACA 
GACCGGGAAG AGAAAACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135232.1
CDS	243..3923
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279746.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279746.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGAG TGAAAACGCC 
AGTGAGGAGG CTGGTGAGGG TGAATACGTC AATCTGTATT CCTCTGGCCA GAGCAACGGG 
GAACTCCCTC ATTCTGGAGG AGAATCTCCC TCTGTCAAAG ACGGCCATTC CAAAGACTCC 
ACTTCAAACA GCAGTGCCAT GGGGAAGGAA GGCAAAGATG GTGCTGAGAG GCAGCAGCAG 
TCGCCAGATG CTTTGGAATC GTCACAGTTG GAGGAAGAAG TGGATGAGCT CTCCCTTATT 
GACCACAGTG AAATTATGGC TAGGTTAACA CTGAAGCAAG AGGGAGATGA TGGGCCAGAT 
GTCCGCGGTG GATCTGGAGA TATTTTGTTG GTGCATGCAA CAGAGACCGA CAGGAAAGAT 
CTGGTGCTGT ACTGTGAAGC CTTCCTGACT ACATACAGGA CATTTATTAC CCCTGAAGAG 
CTCATCAAGA AGCTGCAGTA CAGATATGAG AAGTTTTGCC ACTTCCCAGA CACGTTTAAG 
AAGCGAGTCA GTAAGAACAC CTTCTTCGTG CTGGTGAGAG TGGTGGATGA GCTGTGCTTA 
GTGGAGCTGA CGGAGGAGAT TCTCAAGCTG CTGATGGACC TGGTGTTCCG GCTGGTCTGC 
AATGGGGAGC TGAGCCTCGC CAGAGTGCTG CGCAAAAACA TCCTGGACAA AGTGGATCAG 
AAGAAGATGT TGAGATACGC CAACTCCATC AAACCTCTCG CAGCCCGCGG GGTGGCAGCC 
AGGCCGGGCA CCTTGCATGA TTTCCACAGT CATGAAATAG CCGAGCAGCT GACACTCTTA 
GATGCTGAAC TCTTCTATAA AATCGAGATA CCAGAAGTTT TGCTTTGGGC GAAAGAGCAA 
AATGAAGAGA AGAGCCCCAA CCTGACGCAG TTCACAGAGC ACTTTAATAA CATGTCCTAC 
TGGGTCCGTT CCATAATAAT GCTTCAAGAG AAAGCCCAAG ACCGAGAGAG GCTGCTGCTT 
AAGTTCATAA AGATTATGAA GCACTTACGA AAGCTGAATA ATTTTAATTC CTATCTGGCC 
ATTCTTTCTG CACTGGATTC TGCACCCATC CGAAGGCTGG AGTGGCAGAA ACAAACCTCA 
GAGGGCCTGG CTGAGTACTG CACACTGATC GACAGCTCCT CGTCCTTCCG CGCCTACCGT 
GCAGCCCTGG CTGATGTGGA GCCTCCCTGC ATCCCATACC TAGGCCTAAT TCTGCAGGAC 
CTGACCTTTG TTCATCTGGG AAACCCAGAC TACATCGACA GCAAGGTGAA CTTCTCCAAG 
CGCTGGCAGC AGTTTAACAT CCTGGACAGC ATGAGGTGCT TCCAACAGGT ACATTACGAC 
ATCAAGAGAA ACGACGACAT CGTGTCGTTC TTCAACGACT TCAGCGACCA TCTGGCGGAG 
GAGGCCCTGT GGGAACTGTC CCTCAAAATC AAACCTCGGA ACATTACAAG GCGGAAAACA 
GACCGGGAAG AGAAAACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279747.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135233.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.

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CloneID	OGa16239
Clone ID Related Accession (Same CDS sequence)	XM_015279747.1 , XM_015279747.2
Accession Version	XM_015279747.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGGA ATCTCCCTCT 
GTCAAAGACG GCCATTCCAA AGACTCCACT TCAAACAGCA GTGCCATGGG GAAGGAAGGC 
AAAGATGGTG CTGAGAGGCA GCAGCAGTCG CCAGATGCTT TGGAATCGTC ACAGTTGGAG 
GAAGAAGTGG ATGAGCTCTC CCTTATTGAC CACAGTGAAA TTATGGCTAG GTTAACACTG 
AAGCAAGAGG GAGATGATGG GCCAGATGTC CGCGGTGGAT CTGGAGATAT TTTGTTGGTG 
CATGCAACAG AGACCGACAG GAAAGATCTG GTGCTGTACT GTGAAGCCTT CCTGACTACA 
TACAGGACAT TTATTACCCC TGAAGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAG 
TTTTGCCACT TCCCAGACAC GTTTAAGAAG CGAGTCAGTA AGAACACCTT CTTCGTGCTG 
GTGAGAGTGG TGGATGAGCT GTGCTTAGTG GAGCTGACGG AGGAGATTCT CAAGCTGCTG 
ATGGACCTGG TGTTCCGGCT GGTCTGCAAT GGGGAGCTGA GCCTCGCCAG AGTGCTGCGC 
AAAAACATCC TGGACAAAGT GGATCAGAAG AAGATGTTGA GATACGCCAA CTCCATCAAA 
CCTCTCGCAG CCCGCGGGGT GGCAGCCAGG CCGGGCACCT TGCATGATTT CCACAGTCAT 
GAAATAGCCG AGCAGCTGAC ACTCTTAGAT GCTGAACTCT TCTATAAAAT CGAGATACCA 
GAAGTTTTGC TTTGGGCGAA AGAGCAAAAT GAAGAGAAGA GCCCCAACCT GACGCAGTTC 
ACAGAGCACT TTAATAACAT GTCCTACTGG GTCCGTTCCA TAATAATGCT TCAAGAGAAA 
GCCCAAGACC GAGAGAGGCT GCTGCTTAAG TTCATAAAGA TTATGAAGCA CTTACGAAAG 
CTGAATAATT TTAATTCCTA TCTGGCCATT CTTTCTGCAC TGGATTCTGC ACCCATCCGA 
AGGCTGGAGT GGCAGAAACA AACCTCAGAG GGCCTGGCTG AGTACTGCAC ACTGATCGAC 
AGCTCCTCGT CCTTCCGCGC CTACCGTGCA GCCCTGGCTG ATGTGGAGCC TCCCTGCATC 
CCATACCTAG GCCTAATTCT GCAGGACCTG ACCTTTGTTC ATCTGGGAAA CCCAGACTAC 
ATCGACAGCA AGGTGAACTT CTCCAAGCGC TGGCAGCAGT TTAACATCCT GGACAGCATG 
AGGTGCTTCC AACAGGTACA TTACGACATC AAGAGAAACG ACGACATCGT GTCGTTCTTC 
AACGACTTCA GCGACCATCT GGCGGAGGAG GCCCTGTGGG AACTGTCCCT CAAAATCAAA 
CCTCGGAACA TTACAAGGCG GAAAACAGAC CGGGAAGAGA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135233.1
CDS	228..3815
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279747.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279747.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGGA ATCTCCCTCT 
GTCAAAGACG GCCATTCCAA AGACTCCACT TCAAACAGCA GTGCCATGGG GAAGGAAGGC 
AAAGATGGTG CTGAGAGGCA GCAGCAGTCG CCAGATGCTT TGGAATCGTC ACAGTTGGAG 
GAAGAAGTGG ATGAGCTCTC CCTTATTGAC CACAGTGAAA TTATGGCTAG GTTAACACTG 
AAGCAAGAGG GAGATGATGG GCCAGATGTC CGCGGTGGAT CTGGAGATAT TTTGTTGGTG 
CATGCAACAG AGACCGACAG GAAAGATCTG GTGCTGTACT GTGAAGCCTT CCTGACTACA 
TACAGGACAT TTATTACCCC TGAAGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAG 
TTTTGCCACT TCCCAGACAC GTTTAAGAAG CGAGTCAGTA AGAACACCTT CTTCGTGCTG 
GTGAGAGTGG TGGATGAGCT GTGCTTAGTG GAGCTGACGG AGGAGATTCT CAAGCTGCTG 
ATGGACCTGG TGTTCCGGCT GGTCTGCAAT GGGGAGCTGA GCCTCGCCAG AGTGCTGCGC 
AAAAACATCC TGGACAAAGT GGATCAGAAG AAGATGTTGA GATACGCCAA CTCCATCAAA 
CCTCTCGCAG CCCGCGGGGT GGCAGCCAGG CCGGGCACCT TGCATGATTT CCACAGTCAT 
GAAATAGCCG AGCAGCTGAC ACTCTTAGAT GCTGAACTCT TCTATAAAAT CGAGATACCA 
GAAGTTTTGC TTTGGGCGAA AGAGCAAAAT GAAGAGAAGA GCCCCAACCT GACGCAGTTC 
ACAGAGCACT TTAATAACAT GTCCTACTGG GTCCGTTCCA TAATAATGCT TCAAGAGAAA 
GCCCAAGACC GAGAGAGGCT GCTGCTTAAG TTCATAAAGA TTATGAAGCA CTTACGAAAG 
CTGAATAATT TTAATTCCTA TCTGGCCATT CTTTCTGCAC TGGATTCTGC ACCCATCCGA 
AGGCTGGAGT GGCAGAAACA AACCTCAGAG GGCCTGGCTG AGTACTGCAC ACTGATCGAC 
AGCTCCTCGT CCTTCCGCGC CTACCGTGCA GCCCTGGCTG ATGTGGAGCC TCCCTGCATC 
CCATACCTAG GCCTAATTCT GCAGGACCTG ACCTTTGTTC ATCTGGGAAA CCCAGACTAC 
ATCGACAGCA AGGTGAACTT CTCCAAGCGC TGGCAGCAGT TTAACATCCT GGACAGCATG 
AGGTGCTTCC AACAGGTACA TTACGACATC AAGAGAAACG ACGACATCGT GTCGTTCTTC 
AACGACTTCA GCGACCATCT GGCGGAGGAG GCCCTGTGGG AACTGTCCCT CAAAATCAAA 
CCTCGGAACA TTACAAGGCG GAAAACAGAC CGGGAAGAGA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279747.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135233.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

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CloneID	OGa16239
Clone ID Related Accession (Same CDS sequence)	XM_015279747.1 , XM_015279747.2
Accession Version	XM_015279747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGGA ATCTCCCTCT 
GTCAAAGACG GCCATTCCAA AGACTCCACT TCAAACAGCA GTGCCATGGG GAAGGAAGGC 
AAAGATGGTG CTGAGAGGCA GCAGCAGTCG CCAGATGCTT TGGAATCGTC ACAGTTGGAG 
GAAGAAGTGG ATGAGCTCTC CCTTATTGAC CACAGTGAAA TTATGGCTAG GTTAACACTG 
AAGCAAGAGG GAGATGATGG GCCAGATGTC CGCGGTGGAT CTGGAGATAT TTTGTTGGTG 
CATGCAACAG AGACCGACAG GAAAGATCTG GTGCTGTACT GTGAAGCCTT CCTGACTACA 
TACAGGACAT TTATTACCCC TGAAGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAG 
TTTTGCCACT TCCCAGACAC GTTTAAGAAG CGAGTCAGTA AGAACACCTT CTTCGTGCTG 
GTGAGAGTGG TGGATGAGCT GTGCTTAGTG GAGCTGACGG AGGAGATTCT CAAGCTGCTG 
ATGGACCTGG TGTTCCGGCT GGTCTGCAAT GGGGAGCTGA GCCTCGCCAG AGTGCTGCGC 
AAAAACATCC TGGACAAAGT GGATCAGAAG AAGATGTTGA GATACGCCAA CTCCATCAAA 
CCTCTCGCAG CCCGCGGGGT GGCAGCCAGG CCGGGCACCT TGCATGATTT CCACAGTCAT 
GAAATAGCCG AGCAGCTGAC ACTCTTAGAT GCTGAACTCT TCTATAAAAT CGAGATACCA 
GAAGTTTTGC TTTGGGCGAA AGAGCAAAAT GAAGAGAAGA GCCCCAACCT GACGCAGTTC 
ACAGAGCACT TTAATAACAT GTCCTACTGG GTCCGTTCCA TAATAATGCT TCAAGAGAAA 
GCCCAAGACC GAGAGAGGCT GCTGCTTAAG TTCATAAAGA TTATGAAGCA CTTACGAAAG 
CTGAATAATT TTAATTCCTA TCTGGCCATT CTTTCTGCAC TGGATTCTGC ACCCATCCGA 
AGGCTGGAGT GGCAGAAACA AACCTCAGAG GGCCTGGCTG AGTACTGCAC ACTGATCGAC 
AGCTCCTCGT CCTTCCGCGC CTACCGTGCA GCCCTGGCTG ATGTGGAGCC TCCCTGCATC 
CCATACCTAG GCCTAATTCT GCAGGACCTG ACCTTTGTTC ATCTGGGAAA CCCAGACTAC 
ATCGACAGCA AGGTGAACTT CTCCAAGCGC TGGCAGCAGT TTAACATCCT GGACAGCATG 
AGGTGCTTCC AACAGGTACA TTACGACATC AAGAGAAACG ACGACATCGT GTCGTTCTTC 
AACGACTTCA GCGACCATCT GGCGGAGGAG GCCCTGTGGG AACTGTCCCT CAAAATCAAA 
CCTCGGAACA TTACAAGGCG GAAAACAGAC CGGGAAGAGA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135233.1
CDS	243..3830
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279747.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279747.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGTACA AGTCTGTGTT TAGGTCCTAC TCGCAGGACT TTGTGCCTCA CAACCAGGTC 
TCCGTACCTC CTTTCCTGTC TTCTACCTCC TCCTCCTGCT CCACCGCGCC CTTTCCGCCC 
GTCCACCCGT CTCCGAGCTC TGACCTCGCA GTGCCAGCCA CGGCCAGCCC GTCTCCCAGC 
ACTGGGGATG TGCCAAACTC CTCTTCGCAG GAGAGCAGCT TTAACGGGAG TGCTCCTGTC 
TGCCTTCCTT CTGAAACCTC TCTCACTGAT TCTCTGCAGA CGCCTTCGGA ATCTCCCTCT 
GTCAAAGACG GCCATTCCAA AGACTCCACT TCAAACAGCA GTGCCATGGG GAAGGAAGGC 
AAAGATGGTG CTGAGAGGCA GCAGCAGTCG CCAGATGCTT TGGAATCGTC ACAGTTGGAG 
GAAGAAGTGG ATGAGCTCTC CCTTATTGAC CACAGTGAAA TTATGGCTAG GTTAACACTG 
AAGCAAGAGG GAGATGATGG GCCAGATGTC CGCGGTGGAT CTGGAGATAT TTTGTTGGTG 
CATGCAACAG AGACCGACAG GAAAGATCTG GTGCTGTACT GTGAAGCCTT CCTGACTACA 
TACAGGACAT TTATTACCCC TGAAGAGCTC ATCAAGAAGC TGCAGTACAG ATATGAGAAG 
TTTTGCCACT TCCCAGACAC GTTTAAGAAG CGAGTCAGTA AGAACACCTT CTTCGTGCTG 
GTGAGAGTGG TGGATGAGCT GTGCTTAGTG GAGCTGACGG AGGAGATTCT CAAGCTGCTG 
ATGGACCTGG TGTTCCGGCT GGTCTGCAAT GGGGAGCTGA GCCTCGCCAG AGTGCTGCGC 
AAAAACATCC TGGACAAAGT GGATCAGAAG AAGATGTTGA GATACGCCAA CTCCATCAAA 
CCTCTCGCAG CCCGCGGGGT GGCAGCCAGG CCGGGCACCT TGCATGATTT CCACAGTCAT 
GAAATAGCCG AGCAGCTGAC ACTCTTAGAT GCTGAACTCT TCTATAAAAT CGAGATACCA 
GAAGTTTTGC TTTGGGCGAA AGAGCAAAAT GAAGAGAAGA GCCCCAACCT GACGCAGTTC 
ACAGAGCACT TTAATAACAT GTCCTACTGG GTCCGTTCCA TAATAATGCT TCAAGAGAAA 
GCCCAAGACC GAGAGAGGCT GCTGCTTAAG TTCATAAAGA TTATGAAGCA CTTACGAAAG 
CTGAATAATT TTAATTCCTA TCTGGCCATT CTTTCTGCAC TGGATTCTGC ACCCATCCGA 
AGGCTGGAGT GGCAGAAACA AACCTCAGAG GGCCTGGCTG AGTACTGCAC ACTGATCGAC 
AGCTCCTCGT CCTTCCGCGC CTACCGTGCA GCCCTGGCTG ATGTGGAGCC TCCCTGCATC 
CCATACCTAG GCCTAATTCT GCAGGACCTG ACCTTTGTTC ATCTGGGAAA CCCAGACTAC 
ATCGACAGCA AGGTGAACTT CTCCAAGCGC TGGCAGCAGT TTAACATCCT GGACAGCATG 
AGGTGCTTCC AACAGGTACA TTACGACATC AAGAGAAACG ACGACATCGT GTCGTTCTTC 
AACGACTTCA GCGACCATCT GGCGGAGGAG GCCCTGTGGG AACTGTCCCT CAAAATCAAA 
CCTCGGAACA TTACAAGGCG GAAAACAGAC CGGGAAGAGA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279750.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135236.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa16240
Clone ID Related Accession (Same CDS sequence)	XM_015279750.1 , XM_015279750.2
Accession Version	XM_015279750.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGAGTG AAAACGCCAG TGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTGTATTCC 
TCTGGCCAGA GCAACGGGGA ACTCCCTCAT TCTGGAGGAG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135236.1
CDS	227..3625
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279750.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279750.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGAGTG AAAACGCCAG TGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTGTATTCC 
TCTGGCCAGA GCAACGGGGA ACTCCCTCAT TCTGGAGGAG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279750.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135236.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

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CloneID	OGa16240
Clone ID Related Accession (Same CDS sequence)	XM_015279750.1 , XM_015279750.2
Accession Version	XM_015279750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGAGTG AAAACGCCAG TGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTGTATTCC 
TCTGGCCAGA GCAACGGGGA ACTCCCTCAT TCTGGAGGAG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135236.1
CDS	241..3639
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279750.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279750.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGAGTG AAAACGCCAG TGAGGAGGCT GGTGAGGGTG AATACGTCAA TCTGTATTCC 
TCTGGCCAGA GCAACGGGGA ACTCCCTCAT TCTGGAGGAG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279751.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135237.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa16241
Clone ID Related Accession (Same CDS sequence)	XM_015279751.1 , XM_015279751.2
Accession Version	XM_015279751.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGAGT GAAAACGCCA GTGAGGAGGC TGGTGAGGGT 
GAATACGTCA ATCTGTATTC CTCTGGCCAG AGCAACGGGG AACTCCCTCA TTCTGGAGGA 
GAATCTCCCT CTGTCAAAGA CGGCCATTCC AAAGACTCCA CTTCAAACAG CAGTGCCATG 
GGGAAGGAAG GCAAAGATGG TGCTGAGAGG CAGCAGCAGT CGCCAGATGC TTTGGAATCG 
TCACAGTTGG AGGAAGAAGT GGATGAGCTC TCCCTTATTG ACCACAGTGA AATTATGGCT 
AGGTTAACAC TGAAGCAAGA GGGAGATGAT GGGCCAGATG TCCGCGGTGG ATCTGGAGAT 
ATTTTGTTGG TGCATGCAAC AGAGACCGAC AGGAAAGATC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACTA CATACAGGAC ATTTATTACC CCTGAAGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AGTTTTGCCA CTTCCCAGAC ACGTTTAAGA AGCGAGTCAG TAAGAACACC 
TTCTTCGTGC TGGTGAGAGT GGTGGATGAG CTGTGCTTAG TGGAGCTGAC GGAGGAGATT 
CTCAAGCTGC TGATGGACCT GGTGTTCCGG CTGGTCTGCA ATGGGGAGCT GAGCCTCGCC 
AGAGTGCTGC GCAAAAACAT CCTGGACAAA GTGGATCAGA AGAAGATGTT GAGATACGCC 
AACTCCATCA AACCTCTCGC AGCCCGCGGG GTGGCAGCCA GGCCGGGCAC CTTGCATGAT 
TTCCACAGTC ATGAAATAGC CGAGCAGCTG ACACTCTTAG ATGCTGAACT CTTCTATAAA 
ATCGAGATAC CAGAAGTTTT GCTTTGGGCG AAAGAGCAAA ATGAAGAGAA GAGCCCCAAC 
CTGACGCAGT TCACAGAGCA CTTTAATAAC ATGTCCTACT GGGTCCGTTC CATAATAATG 
CTTCAAGAGA AAGCCCAAGA CCGAGAGAGG CTGCTGCTTA AGTTCATAAA GATTATGAAG 
CACTTACGAA AGCTGAATAA TTTTAATTCC TATCTGGCCA TTCTTTCTGC ACTGGATTCT 
GCACCCATCC GAAGGCTGGA GTGGCAGAAA CAAACCTCAG AGGGCCTGGC TGAGTACTGC 
ACACTGATCG ACAGCTCCTC GTCCTTCCGC GCCTACCGTG CAGCCCTGGC TGATGTGGAG 
CCTCCCTGCA TCCCATACCT AGGCCTAATT CTGCAGGACC TGACCTTTGT TCATCTGGGA 
AACCCAGACT ACATCGACAG CAAGGTGAAC TTCTCCAAGC GCTGGCAGCA GTTTAACATC 
CTGGACAGCA TGAGGTGCTT CCAACAGGTA CATTACGACA TCAAGAGAAA CGACGACATC 
GTGTCGTTCT TCAACGACTT CAGCGACCAT CTGGCGGAGG AGGCCCTGTG GGAACTGTCC 
CTCAAAATCA AACCTCGGAA CATTACAAGG CGGAAAACAG ACCGGGAAGA GAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135237.1
CDS	225..3464
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279751.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279751.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGAGT GAAAACGCCA GTGAGGAGGC TGGTGAGGGT 
GAATACGTCA ATCTGTATTC CTCTGGCCAG AGCAACGGGG AACTCCCTCA TTCTGGAGGA 
GAATCTCCCT CTGTCAAAGA CGGCCATTCC AAAGACTCCA CTTCAAACAG CAGTGCCATG 
GGGAAGGAAG GCAAAGATGG TGCTGAGAGG CAGCAGCAGT CGCCAGATGC TTTGGAATCG 
TCACAGTTGG AGGAAGAAGT GGATGAGCTC TCCCTTATTG ACCACAGTGA AATTATGGCT 
AGGTTAACAC TGAAGCAAGA GGGAGATGAT GGGCCAGATG TCCGCGGTGG ATCTGGAGAT 
ATTTTGTTGG TGCATGCAAC AGAGACCGAC AGGAAAGATC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACTA CATACAGGAC ATTTATTACC CCTGAAGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AGTTTTGCCA CTTCCCAGAC ACGTTTAAGA AGCGAGTCAG TAAGAACACC 
TTCTTCGTGC TGGTGAGAGT GGTGGATGAG CTGTGCTTAG TGGAGCTGAC GGAGGAGATT 
CTCAAGCTGC TGATGGACCT GGTGTTCCGG CTGGTCTGCA ATGGGGAGCT GAGCCTCGCC 
AGAGTGCTGC GCAAAAACAT CCTGGACAAA GTGGATCAGA AGAAGATGTT GAGATACGCC 
AACTCCATCA AACCTCTCGC AGCCCGCGGG GTGGCAGCCA GGCCGGGCAC CTTGCATGAT 
TTCCACAGTC ATGAAATAGC CGAGCAGCTG ACACTCTTAG ATGCTGAACT CTTCTATAAA 
ATCGAGATAC CAGAAGTTTT GCTTTGGGCG AAAGAGCAAA ATGAAGAGAA GAGCCCCAAC 
CTGACGCAGT TCACAGAGCA CTTTAATAAC ATGTCCTACT GGGTCCGTTC CATAATAATG 
CTTCAAGAGA AAGCCCAAGA CCGAGAGAGG CTGCTGCTTA AGTTCATAAA GATTATGAAG 
CACTTACGAA AGCTGAATAA TTTTAATTCC TATCTGGCCA TTCTTTCTGC ACTGGATTCT 
GCACCCATCC GAAGGCTGGA GTGGCAGAAA CAAACCTCAG AGGGCCTGGC TGAGTACTGC 
ACACTGATCG ACAGCTCCTC GTCCTTCCGC GCCTACCGTG CAGCCCTGGC TGATGTGGAG 
CCTCCCTGCA TCCCATACCT AGGCCTAATT CTGCAGGACC TGACCTTTGT TCATCTGGGA 
AACCCAGACT ACATCGACAG CAAGGTGAAC TTCTCCAAGC GCTGGCAGCA GTTTAACATC 
CTGGACAGCA TGAGGTGCTT CCAACAGGTA CATTACGACA TCAAGAGAAA CGACGACATC 
GTGTCGTTCT TCAACGACTT CAGCGACCAT CTGGCGGAGG AGGCCCTGTG GGAACTGTCC 
CTCAAAATCA AACCTCGGAA CATTACAAGG CGGAAAACAG ACCGGGAAGA GAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279751.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135237.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa16241
Clone ID Related Accession (Same CDS sequence)	XM_015279751.1 , XM_015279751.2
Accession Version	XM_015279751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGAGT GAAAACGCCA GTGAGGAGGC TGGTGAGGGT 
GAATACGTCA ATCTGTATTC CTCTGGCCAG AGCAACGGGG AACTCCCTCA TTCTGGAGGA 
GAATCTCCCT CTGTCAAAGA CGGCCATTCC AAAGACTCCA CTTCAAACAG CAGTGCCATG 
GGGAAGGAAG GCAAAGATGG TGCTGAGAGG CAGCAGCAGT CGCCAGATGC TTTGGAATCG 
TCACAGTTGG AGGAAGAAGT GGATGAGCTC TCCCTTATTG ACCACAGTGA AATTATGGCT 
AGGTTAACAC TGAAGCAAGA GGGAGATGAT GGGCCAGATG TCCGCGGTGG ATCTGGAGAT 
ATTTTGTTGG TGCATGCAAC AGAGACCGAC AGGAAAGATC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACTA CATACAGGAC ATTTATTACC CCTGAAGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AGTTTTGCCA CTTCCCAGAC ACGTTTAAGA AGCGAGTCAG TAAGAACACC 
TTCTTCGTGC TGGTGAGAGT GGTGGATGAG CTGTGCTTAG TGGAGCTGAC GGAGGAGATT 
CTCAAGCTGC TGATGGACCT GGTGTTCCGG CTGGTCTGCA ATGGGGAGCT GAGCCTCGCC 
AGAGTGCTGC GCAAAAACAT CCTGGACAAA GTGGATCAGA AGAAGATGTT GAGATACGCC 
AACTCCATCA AACCTCTCGC AGCCCGCGGG GTGGCAGCCA GGCCGGGCAC CTTGCATGAT 
TTCCACAGTC ATGAAATAGC CGAGCAGCTG ACACTCTTAG ATGCTGAACT CTTCTATAAA 
ATCGAGATAC CAGAAGTTTT GCTTTGGGCG AAAGAGCAAA ATGAAGAGAA GAGCCCCAAC 
CTGACGCAGT TCACAGAGCA CTTTAATAAC ATGTCCTACT GGGTCCGTTC CATAATAATG 
CTTCAAGAGA AAGCCCAAGA CCGAGAGAGG CTGCTGCTTA AGTTCATAAA GATTATGAAG 
CACTTACGAA AGCTGAATAA TTTTAATTCC TATCTGGCCA TTCTTTCTGC ACTGGATTCT 
GCACCCATCC GAAGGCTGGA GTGGCAGAAA CAAACCTCAG AGGGCCTGGC TGAGTACTGC 
ACACTGATCG ACAGCTCCTC GTCCTTCCGC GCCTACCGTG CAGCCCTGGC TGATGTGGAG 
CCTCCCTGCA TCCCATACCT AGGCCTAATT CTGCAGGACC TGACCTTTGT TCATCTGGGA 
AACCCAGACT ACATCGACAG CAAGGTGAAC TTCTCCAAGC GCTGGCAGCA GTTTAACATC 
CTGGACAGCA TGAGGTGCTT CCAACAGGTA CATTACGACA TCAAGAGAAA CGACGACATC 
GTGTCGTTCT TCAACGACTT CAGCGACCAT CTGGCGGAGG AGGCCCTGTG GGAACTGTCC 
CTCAAAATCA AACCTCGGAA CATTACAAGG CGGAAAACAG ACCGGGAAGA GAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135237.1
CDS	239..3478
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279751.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279751.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGAGT GAAAACGCCA GTGAGGAGGC TGGTGAGGGT 
GAATACGTCA ATCTGTATTC CTCTGGCCAG AGCAACGGGG AACTCCCTCA TTCTGGAGGA 
GAATCTCCCT CTGTCAAAGA CGGCCATTCC AAAGACTCCA CTTCAAACAG CAGTGCCATG 
GGGAAGGAAG GCAAAGATGG TGCTGAGAGG CAGCAGCAGT CGCCAGATGC TTTGGAATCG 
TCACAGTTGG AGGAAGAAGT GGATGAGCTC TCCCTTATTG ACCACAGTGA AATTATGGCT 
AGGTTAACAC TGAAGCAAGA GGGAGATGAT GGGCCAGATG TCCGCGGTGG ATCTGGAGAT 
ATTTTGTTGG TGCATGCAAC AGAGACCGAC AGGAAAGATC TGGTGCTGTA CTGTGAAGCC 
TTCCTGACTA CATACAGGAC ATTTATTACC CCTGAAGAGC TCATCAAGAA GCTGCAGTAC 
AGATATGAGA AGTTTTGCCA CTTCCCAGAC ACGTTTAAGA AGCGAGTCAG TAAGAACACC 
TTCTTCGTGC TGGTGAGAGT GGTGGATGAG CTGTGCTTAG TGGAGCTGAC GGAGGAGATT 
CTCAAGCTGC TGATGGACCT GGTGTTCCGG CTGGTCTGCA ATGGGGAGCT GAGCCTCGCC 
AGAGTGCTGC GCAAAAACAT CCTGGACAAA GTGGATCAGA AGAAGATGTT GAGATACGCC 
AACTCCATCA AACCTCTCGC AGCCCGCGGG GTGGCAGCCA GGCCGGGCAC CTTGCATGAT 
TTCCACAGTC ATGAAATAGC CGAGCAGCTG ACACTCTTAG ATGCTGAACT CTTCTATAAA 
ATCGAGATAC CAGAAGTTTT GCTTTGGGCG AAAGAGCAAA ATGAAGAGAA GAGCCCCAAC 
CTGACGCAGT TCACAGAGCA CTTTAATAAC ATGTCCTACT GGGTCCGTTC CATAATAATG 
CTTCAAGAGA AAGCCCAAGA CCGAGAGAGG CTGCTGCTTA AGTTCATAAA GATTATGAAG 
CACTTACGAA AGCTGAATAA TTTTAATTCC TATCTGGCCA TTCTTTCTGC ACTGGATTCT 
GCACCCATCC GAAGGCTGGA GTGGCAGAAA CAAACCTCAG AGGGCCTGGC TGAGTACTGC 
ACACTGATCG ACAGCTCCTC GTCCTTCCGC GCCTACCGTG CAGCCCTGGC TGATGTGGAG 
CCTCCCTGCA TCCCATACCT AGGCCTAATT CTGCAGGACC TGACCTTTGT TCATCTGGGA 
AACCCAGACT ACATCGACAG CAAGGTGAAC TTCTCCAAGC GCTGGCAGCA GTTTAACATC 
CTGGACAGCA TGAGGTGCTT CCAACAGGTA CATTACGACA TCAAGAGAAA CGACGACATC 
GTGTCGTTCT TCAACGACTT CAGCGACCAT CTGGCGGAGG AGGCCCTGTG GGAACTGTCC 
CTCAAAATCA AACCTCGGAA CATTACAAGG CGGAAAACAG ACCGGGAAGA GAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279752.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135238.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa16242
Clone ID Related Accession (Same CDS sequence)	XM_015279752.2 , XM_015279752.1
Accession Version	XM_015279752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGAA TCTCCCTCTG TCAAAGACGG CCATTCCAAA 
GACTCCACTT CAAACAGCAG TGCCATGGGG AAGGAAGGCA AAGATGGTGC TGAGAGGCAG 
CAGCAGTCGC CAGATGCTTT GGAATCGTCA CAGTTGGAGG AAGAAGTGGA TGAGCTCTCC 
CTTATTGACC ACAGTGAAAT TATGGCTAGG TTAACACTGA AGCAAGAGGG AGATGATGGG 
CCAGATGTCC GCGGTGGATC TGGAGATATT TTGTTGGTGC ATGCAACAGA GACCGACAGG 
AAAGATCTGG TGCTGTACTG TGAAGCCTTC CTGACTACAT ACAGGACATT TATTACCCCT 
GAAGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAGT TTTGCCACTT CCCAGACACG 
TTTAAGAAGC GAGTCAGTAA GAACACCTTC TTCGTGCTGG TGAGAGTGGT GGATGAGCTG 
TGCTTAGTGG AGCTGACGGA GGAGATTCTC AAGCTGCTGA TGGACCTGGT GTTCCGGCTG 
GTCTGCAATG GGGAGCTGAG CCTCGCCAGA GTGCTGCGCA AAAACATCCT GGACAAAGTG 
GATCAGAAGA AGATGTTGAG ATACGCCAAC TCCATCAAAC CTCTCGCAGC CCGCGGGGTG 
GCAGCCAGGC CGGGCACCTT GCATGATTTC CACAGTCATG AAATAGCCGA GCAGCTGACA 
CTCTTAGATG CTGAACTCTT CTATAAAATC GAGATACCAG AAGTTTTGCT TTGGGCGAAA 
GAGCAAAATG AAGAGAAGAG CCCCAACCTG ACGCAGTTCA CAGAGCACTT TAATAACATG 
TCCTACTGGG TCCGTTCCAT AATAATGCTT CAAGAGAAAG CCCAAGACCG AGAGAGGCTG 
CTGCTTAAGT TCATAAAGAT TATGAAGCAC TTACGAAAGC TGAATAATTT TAATTCCTAT 
CTGGCCATTC TTTCTGCACT GGATTCTGCA CCCATCCGAA GGCTGGAGTG GCAGAAACAA 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA CTGATCGACA GCTCCTCGTC CTTCCGCGCC 
TACCGTGCAG CCCTGGCTGA TGTGGAGCCT CCCTGCATCC CATACCTAGG CCTAATTCTG 
CAGGACCTGA CCTTTGTTCA TCTGGGAAAC CCAGACTACA TCGACAGCAA GGTGAACTTC 
TCCAAGCGCT GGCAGCAGTT TAACATCCTG GACAGCATGA GGTGCTTCCA ACAGGTACAT 
TACGACATCA AGAGAAACGA CGACATCGTG TCGTTCTTCA ACGACTTCAG CGACCATCTG 
GCGGAGGAGG CCCTGTGGGA ACTGTCCCTC AAAATCAAAC CTCGGAACAT TACAAGGCGG 
AAAACAGACC GGGAAGAGAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135238.1
CDS	239..3385
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279752.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279752.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGAA TCTCCCTCTG TCAAAGACGG CCATTCCAAA 
GACTCCACTT CAAACAGCAG TGCCATGGGG AAGGAAGGCA AAGATGGTGC TGAGAGGCAG 
CAGCAGTCGC CAGATGCTTT GGAATCGTCA CAGTTGGAGG AAGAAGTGGA TGAGCTCTCC 
CTTATTGACC ACAGTGAAAT TATGGCTAGG TTAACACTGA AGCAAGAGGG AGATGATGGG 
CCAGATGTCC GCGGTGGATC TGGAGATATT TTGTTGGTGC ATGCAACAGA GACCGACAGG 
AAAGATCTGG TGCTGTACTG TGAAGCCTTC CTGACTACAT ACAGGACATT TATTACCCCT 
GAAGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAGT TTTGCCACTT CCCAGACACG 
TTTAAGAAGC GAGTCAGTAA GAACACCTTC TTCGTGCTGG TGAGAGTGGT GGATGAGCTG 
TGCTTAGTGG AGCTGACGGA GGAGATTCTC AAGCTGCTGA TGGACCTGGT GTTCCGGCTG 
GTCTGCAATG GGGAGCTGAG CCTCGCCAGA GTGCTGCGCA AAAACATCCT GGACAAAGTG 
GATCAGAAGA AGATGTTGAG ATACGCCAAC TCCATCAAAC CTCTCGCAGC CCGCGGGGTG 
GCAGCCAGGC CGGGCACCTT GCATGATTTC CACAGTCATG AAATAGCCGA GCAGCTGACA 
CTCTTAGATG CTGAACTCTT CTATAAAATC GAGATACCAG AAGTTTTGCT TTGGGCGAAA 
GAGCAAAATG AAGAGAAGAG CCCCAACCTG ACGCAGTTCA CAGAGCACTT TAATAACATG 
TCCTACTGGG TCCGTTCCAT AATAATGCTT CAAGAGAAAG CCCAAGACCG AGAGAGGCTG 
CTGCTTAAGT TCATAAAGAT TATGAAGCAC TTACGAAAGC TGAATAATTT TAATTCCTAT 
CTGGCCATTC TTTCTGCACT GGATTCTGCA CCCATCCGAA GGCTGGAGTG GCAGAAACAA 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA CTGATCGACA GCTCCTCGTC CTTCCGCGCC 
TACCGTGCAG CCCTGGCTGA TGTGGAGCCT CCCTGCATCC CATACCTAGG CCTAATTCTG 
CAGGACCTGA CCTTTGTTCA TCTGGGAAAC CCAGACTACA TCGACAGCAA GGTGAACTTC 
TCCAAGCGCT GGCAGCAGTT TAACATCCTG GACAGCATGA GGTGCTTCCA ACAGGTACAT 
TACGACATCA AGAGAAACGA CGACATCGTG TCGTTCTTCA ACGACTTCAG CGACCATCTG 
GCGGAGGAGG CCCTGTGGGA ACTGTCCCTC AAAATCAAAC CTCGGAACAT TACAAGGCGG 
AAAACAGACC GGGAAGAGAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279752.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135238.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa16242
Clone ID Related Accession (Same CDS sequence)	XM_015279752.2 , XM_015279752.1
Accession Version	XM_015279752.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGAA TCTCCCTCTG TCAAAGACGG CCATTCCAAA 
GACTCCACTT CAAACAGCAG TGCCATGGGG AAGGAAGGCA AAGATGGTGC TGAGAGGCAG 
CAGCAGTCGC CAGATGCTTT GGAATCGTCA CAGTTGGAGG AAGAAGTGGA TGAGCTCTCC 
CTTATTGACC ACAGTGAAAT TATGGCTAGG TTAACACTGA AGCAAGAGGG AGATGATGGG 
CCAGATGTCC GCGGTGGATC TGGAGATATT TTGTTGGTGC ATGCAACAGA GACCGACAGG 
AAAGATCTGG TGCTGTACTG TGAAGCCTTC CTGACTACAT ACAGGACATT TATTACCCCT 
GAAGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAGT TTTGCCACTT CCCAGACACG 
TTTAAGAAGC GAGTCAGTAA GAACACCTTC TTCGTGCTGG TGAGAGTGGT GGATGAGCTG 
TGCTTAGTGG AGCTGACGGA GGAGATTCTC AAGCTGCTGA TGGACCTGGT GTTCCGGCTG 
GTCTGCAATG GGGAGCTGAG CCTCGCCAGA GTGCTGCGCA AAAACATCCT GGACAAAGTG 
GATCAGAAGA AGATGTTGAG ATACGCCAAC TCCATCAAAC CTCTCGCAGC CCGCGGGGTG 
GCAGCCAGGC CGGGCACCTT GCATGATTTC CACAGTCATG AAATAGCCGA GCAGCTGACA 
CTCTTAGATG CTGAACTCTT CTATAAAATC GAGATACCAG AAGTTTTGCT TTGGGCGAAA 
GAGCAAAATG AAGAGAAGAG CCCCAACCTG ACGCAGTTCA CAGAGCACTT TAATAACATG 
TCCTACTGGG TCCGTTCCAT AATAATGCTT CAAGAGAAAG CCCAAGACCG AGAGAGGCTG 
CTGCTTAAGT TCATAAAGAT TATGAAGCAC TTACGAAAGC TGAATAATTT TAATTCCTAT 
CTGGCCATTC TTTCTGCACT GGATTCTGCA CCCATCCGAA GGCTGGAGTG GCAGAAACAA 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA CTGATCGACA GCTCCTCGTC CTTCCGCGCC 
TACCGTGCAG CCCTGGCTGA TGTGGAGCCT CCCTGCATCC CATACCTAGG CCTAATTCTG 
CAGGACCTGA CCTTTGTTCA TCTGGGAAAC CCAGACTACA TCGACAGCAA GGTGAACTTC 
TCCAAGCGCT GGCAGCAGTT TAACATCCTG GACAGCATGA GGTGCTTCCA ACAGGTACAT 
TACGACATCA AGAGAAACGA CGACATCGTG TCGTTCTTCA ACGACTTCAG CGACCATCTG 
GCGGAGGAGG CCCTGTGGGA ACTGTCCCTC AAAATCAAAC CTCGGAACAT TACAAGGCGG 
AAAACAGACC GGGAAGAGAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135238.1
CDS	225..3371
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279752.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279752.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGAA TCTCCCTCTG TCAAAGACGG CCATTCCAAA 
GACTCCACTT CAAACAGCAG TGCCATGGGG AAGGAAGGCA AAGATGGTGC TGAGAGGCAG 
CAGCAGTCGC CAGATGCTTT GGAATCGTCA CAGTTGGAGG AAGAAGTGGA TGAGCTCTCC 
CTTATTGACC ACAGTGAAAT TATGGCTAGG TTAACACTGA AGCAAGAGGG AGATGATGGG 
CCAGATGTCC GCGGTGGATC TGGAGATATT TTGTTGGTGC ATGCAACAGA GACCGACAGG 
AAAGATCTGG TGCTGTACTG TGAAGCCTTC CTGACTACAT ACAGGACATT TATTACCCCT 
GAAGAGCTCA TCAAGAAGCT GCAGTACAGA TATGAGAAGT TTTGCCACTT CCCAGACACG 
TTTAAGAAGC GAGTCAGTAA GAACACCTTC TTCGTGCTGG TGAGAGTGGT GGATGAGCTG 
TGCTTAGTGG AGCTGACGGA GGAGATTCTC AAGCTGCTGA TGGACCTGGT GTTCCGGCTG 
GTCTGCAATG GGGAGCTGAG CCTCGCCAGA GTGCTGCGCA AAAACATCCT GGACAAAGTG 
GATCAGAAGA AGATGTTGAG ATACGCCAAC TCCATCAAAC CTCTCGCAGC CCGCGGGGTG 
GCAGCCAGGC CGGGCACCTT GCATGATTTC CACAGTCATG AAATAGCCGA GCAGCTGACA 
CTCTTAGATG CTGAACTCTT CTATAAAATC GAGATACCAG AAGTTTTGCT TTGGGCGAAA 
GAGCAAAATG AAGAGAAGAG CCCCAACCTG ACGCAGTTCA CAGAGCACTT TAATAACATG 
TCCTACTGGG TCCGTTCCAT AATAATGCTT CAAGAGAAAG CCCAAGACCG AGAGAGGCTG 
CTGCTTAAGT TCATAAAGAT TATGAAGCAC TTACGAAAGC TGAATAATTT TAATTCCTAT 
CTGGCCATTC TTTCTGCACT GGATTCTGCA CCCATCCGAA GGCTGGAGTG GCAGAAACAA 
ACCTCAGAGG GCCTGGCTGA GTACTGCACA CTGATCGACA GCTCCTCGTC CTTCCGCGCC 
TACCGTGCAG CCCTGGCTGA TGTGGAGCCT CCCTGCATCC CATACCTAGG CCTAATTCTG 
CAGGACCTGA CCTTTGTTCA TCTGGGAAAC CCAGACTACA TCGACAGCAA GGTGAACTTC 
TCCAAGCGCT GGCAGCAGTT TAACATCCTG GACAGCATGA GGTGCTTCCA ACAGGTACAT 
TACGACATCA AGAGAAACGA CGACATCGTG TCGTTCTTCA ACGACTTCAG CGACCATCTG 
GCGGAGGAGG CCCTGTGGGA ACTGTCCCTC AAAATCAAAC CTCGGAACAT TACAAGGCGG 
AAAACAGACC GGGAAGAGAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279741.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135227.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa43663
Clone ID Related Accession (Same CDS sequence)	XM_015279741.2 , XM_015279741.1
Accession Version	XM_015279741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGA GTGAAAACGC CAGTGAGGAG 
GCTGGTGAGG GTGAATACGT CAATCTGTAT TCCTCTGGCC AGAGCAACGG GGAACTCCCT 
CATTCTGGAG GAGAATCTCC CTCTGTCAAA GACGGCCATT CCAAAGACTC CACTTCAAAC 
AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT 
GCTTTGGAAT CGTCACAGTT GGAGGAAGAA GTGGATGAGC TCTCCCTTAT TGACCACAGT 
GAAATTATGG CTAGGTTAAC ACTGAAGCAA GAGGGAGATG ATGGGCCAGA TGTCCGCGGT 
GGATCTGGAG ATATTTTGTT GGTGCATGCA ACAGAGACCG ACAGGAAAGA TCTGGTGCTG 
TACTGTGAAG CCTTCCTGAC TACATACAGG ACATTTATTA CCCCTGAAGA GCTCATCAAG 
AAGCTGCAGT ACAGATATGA GAAGTTTTGC CACTTCCCAG ACACGTTTAA GAAGCGAGTC 
AGTAAGAACA CCTTCTTCGT GCTGGTGAGA GTGGTGGATG AGCTGTGCTT AGTGGAGCTG 
ACGGAGGAGA TTCTCAAGCT GCTGATGGAC CTGGTGTTCC GGCTGGTCTG CAATGGGGAG 
CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC ATCCTGGACA AAGTGGATCA GAAGAAGATG 
TTGAGATACG CCAACTCCAT CAAACCTCTC GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC 
ACCTTGCATG ATTTCCACAG TCATGAAATA GCCGAGCAGC TGACACTCTT AGATGCTGAA 
CTCTTCTATA AAATCGAGAT ACCAGAAGTT TTGCTTTGGG CGAAAGAGCA AAATGAAGAG 
AAGAGCCCCA ACCTGACGCA GTTCACAGAG CACTTTAATA ACATGTCCTA CTGGGTCCGT 
TCCATAATAA TGCTTCAAGA GAAAGCCCAA GACCGAGAGA GGCTGCTGCT TAAGTTCATA 
AAGATTATGA AGCACTTACG AAAGCTGAAT AATTTTAATT CCTATCTGGC CATTCTTTCT 
GCACTGGATT CTGCACCCAT CCGAAGGCTG GAGTGGCAGA AACAAACCTC AGAGGGCCTG 
GCTGAGTACT GCACACTGAT CGACAGCTCC TCGTCCTTCC GCGCCTACCG TGCAGCCCTG 
GCTGATGTGG AGCCTCCCTG CATCCCATAC CTAGGCCTAA TTCTGCAGGA CCTGACCTTT 
GTTCATCTGG GAAACCCAGA CTACATCGAC AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG 
CAGTTTAACA TCCTGGACAG CATGAGGTGC TTCCAACAGG TACATTACGA CATCAAGAGA 
AACGACGACA TCGTGTCGTT CTTCAACGAC TTCAGCGACC ATCTGGCGGA GGAGGCCCTG 
TGGGAACTGT CCCTCAAAAT CAAACCTCGG AACATTACAA GGCGGAAAAC AGACCGGGAA 
GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135227.1
CDS	245..4216
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279741.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279741.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGA GTGAAAACGC CAGTGAGGAG 
GCTGGTGAGG GTGAATACGT CAATCTGTAT TCCTCTGGCC AGAGCAACGG GGAACTCCCT 
CATTCTGGAG GAGAATCTCC CTCTGTCAAA GACGGCCATT CCAAAGACTC CACTTCAAAC 
AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT 
GCTTTGGAAT CGTCACAGTT GGAGGAAGAA GTGGATGAGC TCTCCCTTAT TGACCACAGT 
GAAATTATGG CTAGGTTAAC ACTGAAGCAA GAGGGAGATG ATGGGCCAGA TGTCCGCGGT 
GGATCTGGAG ATATTTTGTT GGTGCATGCA ACAGAGACCG ACAGGAAAGA TCTGGTGCTG 
TACTGTGAAG CCTTCCTGAC TACATACAGG ACATTTATTA CCCCTGAAGA GCTCATCAAG 
AAGCTGCAGT ACAGATATGA GAAGTTTTGC CACTTCCCAG ACACGTTTAA GAAGCGAGTC 
AGTAAGAACA CCTTCTTCGT GCTGGTGAGA GTGGTGGATG AGCTGTGCTT AGTGGAGCTG 
ACGGAGGAGA TTCTCAAGCT GCTGATGGAC CTGGTGTTCC GGCTGGTCTG CAATGGGGAG 
CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC ATCCTGGACA AAGTGGATCA GAAGAAGATG 
TTGAGATACG CCAACTCCAT CAAACCTCTC GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC 
ACCTTGCATG ATTTCCACAG TCATGAAATA GCCGAGCAGC TGACACTCTT AGATGCTGAA 
CTCTTCTATA AAATCGAGAT ACCAGAAGTT TTGCTTTGGG CGAAAGAGCA AAATGAAGAG 
AAGAGCCCCA ACCTGACGCA GTTCACAGAG CACTTTAATA ACATGTCCTA CTGGGTCCGT 
TCCATAATAA TGCTTCAAGA GAAAGCCCAA GACCGAGAGA GGCTGCTGCT TAAGTTCATA 
AAGATTATGA AGCACTTACG AAAGCTGAAT AATTTTAATT CCTATCTGGC CATTCTTTCT 
GCACTGGATT CTGCACCCAT CCGAAGGCTG GAGTGGCAGA AACAAACCTC AGAGGGCCTG 
GCTGAGTACT GCACACTGAT CGACAGCTCC TCGTCCTTCC GCGCCTACCG TGCAGCCCTG 
GCTGATGTGG AGCCTCCCTG CATCCCATAC CTAGGCCTAA TTCTGCAGGA CCTGACCTTT 
GTTCATCTGG GAAACCCAGA CTACATCGAC AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG 
CAGTTTAACA TCCTGGACAG CATGAGGTGC TTCCAACAGG TACATTACGA CATCAAGAGA 
AACGACGACA TCGTGTCGTT CTTCAACGAC TTCAGCGACC ATCTGGCGGA GGAGGCCCTG 
TGGGAACTGT CCCTCAAAAT CAAACCTCGG AACATTACAA GGCGGAAAAC AGACCGGGAA 
GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279741.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135227.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa43663
Clone ID Related Accession (Same CDS sequence)	XM_015279741.2 , XM_015279741.1
Accession Version	XM_015279741.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGA GTGAAAACGC CAGTGAGGAG 
GCTGGTGAGG GTGAATACGT CAATCTGTAT TCCTCTGGCC AGAGCAACGG GGAACTCCCT 
CATTCTGGAG GAGAATCTCC CTCTGTCAAA GACGGCCATT CCAAAGACTC CACTTCAAAC 
AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT 
GCTTTGGAAT CGTCACAGTT GGAGGAAGAA GTGGATGAGC TCTCCCTTAT TGACCACAGT 
GAAATTATGG CTAGGTTAAC ACTGAAGCAA GAGGGAGATG ATGGGCCAGA TGTCCGCGGT 
GGATCTGGAG ATATTTTGTT GGTGCATGCA ACAGAGACCG ACAGGAAAGA TCTGGTGCTG 
TACTGTGAAG CCTTCCTGAC TACATACAGG ACATTTATTA CCCCTGAAGA GCTCATCAAG 
AAGCTGCAGT ACAGATATGA GAAGTTTTGC CACTTCCCAG ACACGTTTAA GAAGCGAGTC 
AGTAAGAACA CCTTCTTCGT GCTGGTGAGA GTGGTGGATG AGCTGTGCTT AGTGGAGCTG 
ACGGAGGAGA TTCTCAAGCT GCTGATGGAC CTGGTGTTCC GGCTGGTCTG CAATGGGGAG 
CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC ATCCTGGACA AAGTGGATCA GAAGAAGATG 
TTGAGATACG CCAACTCCAT CAAACCTCTC GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC 
ACCTTGCATG ATTTCCACAG TCATGAAATA GCCGAGCAGC TGACACTCTT AGATGCTGAA 
CTCTTCTATA AAATCGAGAT ACCAGAAGTT TTGCTTTGGG CGAAAGAGCA AAATGAAGAG 
AAGAGCCCCA ACCTGACGCA GTTCACAGAG CACTTTAATA ACATGTCCTA CTGGGTCCGT 
TCCATAATAA TGCTTCAAGA GAAAGCCCAA GACCGAGAGA GGCTGCTGCT TAAGTTCATA 
AAGATTATGA AGCACTTACG AAAGCTGAAT AATTTTAATT CCTATCTGGC CATTCTTTCT 
GCACTGGATT CTGCACCCAT CCGAAGGCTG GAGTGGCAGA AACAAACCTC AGAGGGCCTG 
GCTGAGTACT GCACACTGAT CGACAGCTCC TCGTCCTTCC GCGCCTACCG TGCAGCCCTG 
GCTGATGTGG AGCCTCCCTG CATCCCATAC CTAGGCCTAA TTCTGCAGGA CCTGACCTTT 
GTTCATCTGG GAAACCCAGA CTACATCGAC AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG 
CAGTTTAACA TCCTGGACAG CATGAGGTGC TTCCAACAGG TACATTACGA CATCAAGAGA 
AACGACGACA TCGTGTCGTT CTTCAACGAC TTCAGCGACC ATCTGGCGGA GGAGGCCCTG 
TGGGAACTGT CCCTCAAAAT CAAACCTCGG AACATTACAA GGCGGAAAAC AGACCGGGAA 
GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135227.1
CDS	229..4200
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279741.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279741.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGA GTGAAAACGC CAGTGAGGAG 
GCTGGTGAGG GTGAATACGT CAATCTGTAT TCCTCTGGCC AGAGCAACGG GGAACTCCCT 
CATTCTGGAG GAGAATCTCC CTCTGTCAAA GACGGCCATT CCAAAGACTC CACTTCAAAC 
AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT 
GCTTTGGAAT CGTCACAGTT GGAGGAAGAA GTGGATGAGC TCTCCCTTAT TGACCACAGT 
GAAATTATGG CTAGGTTAAC ACTGAAGCAA GAGGGAGATG ATGGGCCAGA TGTCCGCGGT 
GGATCTGGAG ATATTTTGTT GGTGCATGCA ACAGAGACCG ACAGGAAAGA TCTGGTGCTG 
TACTGTGAAG CCTTCCTGAC TACATACAGG ACATTTATTA CCCCTGAAGA GCTCATCAAG 
AAGCTGCAGT ACAGATATGA GAAGTTTTGC CACTTCCCAG ACACGTTTAA GAAGCGAGTC 
AGTAAGAACA CCTTCTTCGT GCTGGTGAGA GTGGTGGATG AGCTGTGCTT AGTGGAGCTG 
ACGGAGGAGA TTCTCAAGCT GCTGATGGAC CTGGTGTTCC GGCTGGTCTG CAATGGGGAG 
CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC ATCCTGGACA AAGTGGATCA GAAGAAGATG 
TTGAGATACG CCAACTCCAT CAAACCTCTC GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC 
ACCTTGCATG ATTTCCACAG TCATGAAATA GCCGAGCAGC TGACACTCTT AGATGCTGAA 
CTCTTCTATA AAATCGAGAT ACCAGAAGTT TTGCTTTGGG CGAAAGAGCA AAATGAAGAG 
AAGAGCCCCA ACCTGACGCA GTTCACAGAG CACTTTAATA ACATGTCCTA CTGGGTCCGT 
TCCATAATAA TGCTTCAAGA GAAAGCCCAA GACCGAGAGA GGCTGCTGCT TAAGTTCATA 
AAGATTATGA AGCACTTACG AAAGCTGAAT AATTTTAATT CCTATCTGGC CATTCTTTCT 
GCACTGGATT CTGCACCCAT CCGAAGGCTG GAGTGGCAGA AACAAACCTC AGAGGGCCTG 
GCTGAGTACT GCACACTGAT CGACAGCTCC TCGTCCTTCC GCGCCTACCG TGCAGCCCTG 
GCTGATGTGG AGCCTCCCTG CATCCCATAC CTAGGCCTAA TTCTGCAGGA CCTGACCTTT 
GTTCATCTGG GAAACCCAGA CTACATCGAC AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG 
CAGTTTAACA TCCTGGACAG CATGAGGTGC TTCCAACAGG TACATTACGA CATCAAGAGA 
AACGACGACA TCGTGTCGTT CTTCAACGAC TTCAGCGACC ATCTGGCGGA GGAGGCCCTG 
TGGGAACTGT CCCTCAAAAT CAAACCTCGG AACATTACAA GGCGGAAAAC AGACCGGGAA 
GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279742.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135228.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

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CloneID	OGa43664
Clone ID Related Accession (Same CDS sequence)	XM_015279742.1 , XM_015279742.2
Accession Version	XM_015279742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGGGCTGGA GCAAACGGCA TCTTAAATTG GGATCCAGTG GAAAATCCCA GCAAAGCAGT 
AAGCCCTCAG AGTGCTTTTC CCTGCCGAGA TGCTTCCAGC ACGCTCAGAG CCGTGGTTTC 
TGTATGCCTT CGCATCCCTT CAGCCAGCCT GGTGCTGTCC AGGTGTGCTC TGTTGCTCAC 
AGCTGCAGCA CCAGGCGTCC CTCCGTGCTG ACCGCCCCGC TGCCACCATC CTGCCCTGAG 
CGAGGGCTGC TGTTTGTGGA AGGCCTCACC TCACCACCAA ACCTGCCCGC CCAGTACAAG 
TCTGTGTTTA GGTCCTACTC GCAGGACTTT GTGCCTCACA ACCAGGTCTC CGTACCTCCT 
TTCCTGTCTT CTACCTCCTC CTCCTGCTCC ACCGCGCCCT TTCCGCCCGT CCACCCGTCT 
CCGAGCTCTG ACCTCGCAGT GCCAGCCACG GCCAGCCCGT CTCCCAGCAC TGGGGATGTG 
CCAAACTCCT CTTCGCAGGA GAGCAGCTTT AACGGGAGTG CTCCTGTCTG CCTTCCTTCT 
GAAACCTCTC TCACTGATTC TCTGCAGACG CCTTCGAGTG AAAACGCCAG TGAGGAGGCT 
GGTGAGGGTG AATACGTCAA TCTGTATTCC TCTGGCCAGA GCAACGGGGA ACTCCCTCAT 
TCTGGAGGAG AATCTCCCTC TGTCAAAGAC GGCCATTCCA AAGACTCCAC TTCAAACAGC 
AGTGCCATGG GGAAGGAAGG CAAAGATGGT GCTGAGAGGC AGCAGCAGTC GCCAGATGCT 
TTGGAATCGT CACAGTTGGA GGAAGAAGTG GATGAGCTCT CCCTTATTGA CCACAGTGAA 
ATTATGGCTA GGTTAACACT GAAGCAAGAG GGAGATGATG GGCCAGATGT CCGCGGTGGA 
TCTGGAGATA TTTTGTTGGT GCATGCAACA GAGACCGACA GGAAAGATCT GGTGCTGTAC 
TGTGAAGCCT TCCTGACTAC ATACAGGACA TTTATTACCC CTGAAGAGCT CATCAAGAAG 
CTGCAGTACA GATATGAGAA GTTTTGCCAC TTCCCAGACA CGTTTAAGAA GCGAGTCAGT 
AAGAACACCT TCTTCGTGCT GGTGAGAGTG GTGGATGAGC TGTGCTTAGT GGAGCTGACG 
GAGGAGATTC TCAAGCTGCT GATGGACCTG GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG 
AGCCTCGCCA GAGTGCTGCG CAAAAACATC CTGGACAAAG TGGATCAGAA GAAGATGTTG 
AGATACGCCA ACTCCATCAA ACCTCTCGCA GCCCGCGGGG TGGCAGCCAG GCCGGGCACC 
TTGCATGATT TCCACAGTCA TGAAATAGCC GAGCAGCTGA CACTCTTAGA TGCTGAACTC 
TTCTATAAAA TCGAGATACC AGAAGTTTTG CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG 
AGCCCCAACC TGACGCAGTT CACAGAGCAC TTTAATAACA TGTCCTACTG GGTCCGTTCC 
ATAATAATGC TTCAAGAGAA AGCCCAAGAC CGAGAGAGGC TGCTGCTTAA GTTCATAAAG 
ATTATGAAGC ACTTACGAAA GCTGAATAAT TTTAATTCCT ATCTGGCCAT TCTTTCTGCA 
CTGGATTCTG CACCCATCCG AAGGCTGGAG TGGCAGAAAC AAACCTCAGA GGGCCTGGCT 
GAGTACTGCA CACTGATCGA CAGCTCCTCG TCCTTCCGCG CCTACCGTGC AGCCCTGGCT 
GATGTGGAGC CTCCCTGCAT CCCATACCTA GGCCTAATTC TGCAGGACCT GACCTTTGTT 
CATCTGGGAA ACCCAGACTA CATCGACAGC AAGGTGAACT TCTCCAAGCG CTGGCAGCAG 
TTTAACATCC TGGACAGCAT GAGGTGCTTC CAACAGGTAC ATTACGACAT CAAGAGAAAC 
GACGACATCG TGTCGTTCTT CAACGACTTC AGCGACCATC TGGCGGAGGA GGCCCTGTGG 
GAACTGTCCC TCAAAATCAA ACCTCGGAAC ATTACAAGGC GGAAAACAGA CCGGGAAGAG 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135228.1
CDS	229..4197
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279742.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279742.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGGGCTGGA GCAAACGGCA TCTTAAATTG GGATCCAGTG GAAAATCCCA GCAAAGCAGT 
AAGCCCTCAG AGTGCTTTTC CCTGCCGAGA TGCTTCCAGC ACGCTCAGAG CCGTGGTTTC 
TGTATGCCTT CGCATCCCTT CAGCCAGCCT GGTGCTGTCC AGGTGTGCTC TGTTGCTCAC 
AGCTGCAGCA CCAGGCGTCC CTCCGTGCTG ACCGCCCCGC TGCCACCATC CTGCCCTGAG 
CGAGGGCTGC TGTTTGTGGA AGGCCTCACC TCACCACCAA ACCTGCCCGC CCAGTACAAG 
TCTGTGTTTA GGTCCTACTC GCAGGACTTT GTGCCTCACA ACCAGGTCTC CGTACCTCCT 
TTCCTGTCTT CTACCTCCTC CTCCTGCTCC ACCGCGCCCT TTCCGCCCGT CCACCCGTCT 
CCGAGCTCTG ACCTCGCAGT GCCAGCCACG GCCAGCCCGT CTCCCAGCAC TGGGGATGTG 
CCAAACTCCT CTTCGCAGGA GAGCAGCTTT AACGGGAGTG CTCCTGTCTG CCTTCCTTCT 
GAAACCTCTC TCACTGATTC TCTGCAGACG CCTTCGAGTG AAAACGCCAG TGAGGAGGCT 
GGTGAGGGTG AATACGTCAA TCTGTATTCC TCTGGCCAGA GCAACGGGGA ACTCCCTCAT 
TCTGGAGGAG AATCTCCCTC TGTCAAAGAC GGCCATTCCA AAGACTCCAC TTCAAACAGC 
AGTGCCATGG GGAAGGAAGG CAAAGATGGT GCTGAGAGGC AGCAGCAGTC GCCAGATGCT 
TTGGAATCGT CACAGTTGGA GGAAGAAGTG GATGAGCTCT CCCTTATTGA CCACAGTGAA 
ATTATGGCTA GGTTAACACT GAAGCAAGAG GGAGATGATG GGCCAGATGT CCGCGGTGGA 
TCTGGAGATA TTTTGTTGGT GCATGCAACA GAGACCGACA GGAAAGATCT GGTGCTGTAC 
TGTGAAGCCT TCCTGACTAC ATACAGGACA TTTATTACCC CTGAAGAGCT CATCAAGAAG 
CTGCAGTACA GATATGAGAA GTTTTGCCAC TTCCCAGACA CGTTTAAGAA GCGAGTCAGT 
AAGAACACCT TCTTCGTGCT GGTGAGAGTG GTGGATGAGC TGTGCTTAGT GGAGCTGACG 
GAGGAGATTC TCAAGCTGCT GATGGACCTG GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG 
AGCCTCGCCA GAGTGCTGCG CAAAAACATC CTGGACAAAG TGGATCAGAA GAAGATGTTG 
AGATACGCCA ACTCCATCAA ACCTCTCGCA GCCCGCGGGG TGGCAGCCAG GCCGGGCACC 
TTGCATGATT TCCACAGTCA TGAAATAGCC GAGCAGCTGA CACTCTTAGA TGCTGAACTC 
TTCTATAAAA TCGAGATACC AGAAGTTTTG CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG 
AGCCCCAACC TGACGCAGTT CACAGAGCAC TTTAATAACA TGTCCTACTG GGTCCGTTCC 
ATAATAATGC TTCAAGAGAA AGCCCAAGAC CGAGAGAGGC TGCTGCTTAA GTTCATAAAG 
ATTATGAAGC ACTTACGAAA GCTGAATAAT TTTAATTCCT ATCTGGCCAT TCTTTCTGCA 
CTGGATTCTG CACCCATCCG AAGGCTGGAG TGGCAGAAAC AAACCTCAGA GGGCCTGGCT 
GAGTACTGCA CACTGATCGA CAGCTCCTCG TCCTTCCGCG CCTACCGTGC AGCCCTGGCT 
GATGTGGAGC CTCCCTGCAT CCCATACCTA GGCCTAATTC TGCAGGACCT GACCTTTGTT 
CATCTGGGAA ACCCAGACTA CATCGACAGC AAGGTGAACT TCTCCAAGCG CTGGCAGCAG 
TTTAACATCC TGGACAGCAT GAGGTGCTTC CAACAGGTAC ATTACGACAT CAAGAGAAAC 
GACGACATCG TGTCGTTCTT CAACGACTTC AGCGACCATC TGGCGGAGGA GGCCCTGTGG 
GAACTGTCCC TCAAAATCAA ACCTCGGAAC ATTACAAGGC GGAAAACAGA CCGGGAAGAG 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279742.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135228.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

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CloneID	OGa43664
Clone ID Related Accession (Same CDS sequence)	XM_015279742.1 , XM_015279742.2
Accession Version	XM_015279742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGGGCTGGA GCAAACGGCA TCTTAAATTG GGATCCAGTG GAAAATCCCA GCAAAGCAGT 
AAGCCCTCAG AGTGCTTTTC CCTGCCGAGA TGCTTCCAGC ACGCTCAGAG CCGTGGTTTC 
TGTATGCCTT CGCATCCCTT CAGCCAGCCT GGTGCTGTCC AGGTGTGCTC TGTTGCTCAC 
AGCTGCAGCA CCAGGCGTCC CTCCGTGCTG ACCGCCCCGC TGCCACCATC CTGCCCTGAG 
CGAGGGCTGC TGTTTGTGGA AGGCCTCACC TCACCACCAA ACCTGCCCGC CCAGTACAAG 
TCTGTGTTTA GGTCCTACTC GCAGGACTTT GTGCCTCACA ACCAGGTCTC CGTACCTCCT 
TTCCTGTCTT CTACCTCCTC CTCCTGCTCC ACCGCGCCCT TTCCGCCCGT CCACCCGTCT 
CCGAGCTCTG ACCTCGCAGT GCCAGCCACG GCCAGCCCGT CTCCCAGCAC TGGGGATGTG 
CCAAACTCCT CTTCGCAGGA GAGCAGCTTT AACGGGAGTG CTCCTGTCTG CCTTCCTTCT 
GAAACCTCTC TCACTGATTC TCTGCAGACG CCTTCGAGTG AAAACGCCAG TGAGGAGGCT 
GGTGAGGGTG AATACGTCAA TCTGTATTCC TCTGGCCAGA GCAACGGGGA ACTCCCTCAT 
TCTGGAGGAG AATCTCCCTC TGTCAAAGAC GGCCATTCCA AAGACTCCAC TTCAAACAGC 
AGTGCCATGG GGAAGGAAGG CAAAGATGGT GCTGAGAGGC AGCAGCAGTC GCCAGATGCT 
TTGGAATCGT CACAGTTGGA GGAAGAAGTG GATGAGCTCT CCCTTATTGA CCACAGTGAA 
ATTATGGCTA GGTTAACACT GAAGCAAGAG GGAGATGATG GGCCAGATGT CCGCGGTGGA 
TCTGGAGATA TTTTGTTGGT GCATGCAACA GAGACCGACA GGAAAGATCT GGTGCTGTAC 
TGTGAAGCCT TCCTGACTAC ATACAGGACA TTTATTACCC CTGAAGAGCT CATCAAGAAG 
CTGCAGTACA GATATGAGAA GTTTTGCCAC TTCCCAGACA CGTTTAAGAA GCGAGTCAGT 
AAGAACACCT TCTTCGTGCT GGTGAGAGTG GTGGATGAGC TGTGCTTAGT GGAGCTGACG 
GAGGAGATTC TCAAGCTGCT GATGGACCTG GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG 
AGCCTCGCCA GAGTGCTGCG CAAAAACATC CTGGACAAAG TGGATCAGAA GAAGATGTTG 
AGATACGCCA ACTCCATCAA ACCTCTCGCA GCCCGCGGGG TGGCAGCCAG GCCGGGCACC 
TTGCATGATT TCCACAGTCA TGAAATAGCC GAGCAGCTGA CACTCTTAGA TGCTGAACTC 
TTCTATAAAA TCGAGATACC AGAAGTTTTG CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG 
AGCCCCAACC TGACGCAGTT CACAGAGCAC TTTAATAACA TGTCCTACTG GGTCCGTTCC 
ATAATAATGC TTCAAGAGAA AGCCCAAGAC CGAGAGAGGC TGCTGCTTAA GTTCATAAAG 
ATTATGAAGC ACTTACGAAA GCTGAATAAT TTTAATTCCT ATCTGGCCAT TCTTTCTGCA 
CTGGATTCTG CACCCATCCG AAGGCTGGAG TGGCAGAAAC AAACCTCAGA GGGCCTGGCT 
GAGTACTGCA CACTGATCGA CAGCTCCTCG TCCTTCCGCG CCTACCGTGC AGCCCTGGCT 
GATGTGGAGC CTCCCTGCAT CCCATACCTA GGCCTAATTC TGCAGGACCT GACCTTTGTT 
CATCTGGGAA ACCCAGACTA CATCGACAGC AAGGTGAACT TCTCCAAGCG CTGGCAGCAG 
TTTAACATCC TGGACAGCAT GAGGTGCTTC CAACAGGTAC ATTACGACAT CAAGAGAAAC 
GACGACATCG TGTCGTTCTT CAACGACTTC AGCGACCATC TGGCGGAGGA GGCCCTGTGG 
GAACTGTCCC TCAAAATCAA ACCTCGGAAC ATTACAAGGC GGAAAACAGA CCGGGAAGAG 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135228.1
CDS	245..4213
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279742.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279742.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGGGCTGGA GCAAACGGCA TCTTAAATTG GGATCCAGTG GAAAATCCCA GCAAAGCAGT 
AAGCCCTCAG AGTGCTTTTC CCTGCCGAGA TGCTTCCAGC ACGCTCAGAG CCGTGGTTTC 
TGTATGCCTT CGCATCCCTT CAGCCAGCCT GGTGCTGTCC AGGTGTGCTC TGTTGCTCAC 
AGCTGCAGCA CCAGGCGTCC CTCCGTGCTG ACCGCCCCGC TGCCACCATC CTGCCCTGAG 
CGAGGGCTGC TGTTTGTGGA AGGCCTCACC TCACCACCAA ACCTGCCCGC CCAGTACAAG 
TCTGTGTTTA GGTCCTACTC GCAGGACTTT GTGCCTCACA ACCAGGTCTC CGTACCTCCT 
TTCCTGTCTT CTACCTCCTC CTCCTGCTCC ACCGCGCCCT TTCCGCCCGT CCACCCGTCT 
CCGAGCTCTG ACCTCGCAGT GCCAGCCACG GCCAGCCCGT CTCCCAGCAC TGGGGATGTG 
CCAAACTCCT CTTCGCAGGA GAGCAGCTTT AACGGGAGTG CTCCTGTCTG CCTTCCTTCT 
GAAACCTCTC TCACTGATTC TCTGCAGACG CCTTCGAGTG AAAACGCCAG TGAGGAGGCT 
GGTGAGGGTG AATACGTCAA TCTGTATTCC TCTGGCCAGA GCAACGGGGA ACTCCCTCAT 
TCTGGAGGAG AATCTCCCTC TGTCAAAGAC GGCCATTCCA AAGACTCCAC TTCAAACAGC 
AGTGCCATGG GGAAGGAAGG CAAAGATGGT GCTGAGAGGC AGCAGCAGTC GCCAGATGCT 
TTGGAATCGT CACAGTTGGA GGAAGAAGTG GATGAGCTCT CCCTTATTGA CCACAGTGAA 
ATTATGGCTA GGTTAACACT GAAGCAAGAG GGAGATGATG GGCCAGATGT CCGCGGTGGA 
TCTGGAGATA TTTTGTTGGT GCATGCAACA GAGACCGACA GGAAAGATCT GGTGCTGTAC 
TGTGAAGCCT TCCTGACTAC ATACAGGACA TTTATTACCC CTGAAGAGCT CATCAAGAAG 
CTGCAGTACA GATATGAGAA GTTTTGCCAC TTCCCAGACA CGTTTAAGAA GCGAGTCAGT 
AAGAACACCT TCTTCGTGCT GGTGAGAGTG GTGGATGAGC TGTGCTTAGT GGAGCTGACG 
GAGGAGATTC TCAAGCTGCT GATGGACCTG GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG 
AGCCTCGCCA GAGTGCTGCG CAAAAACATC CTGGACAAAG TGGATCAGAA GAAGATGTTG 
AGATACGCCA ACTCCATCAA ACCTCTCGCA GCCCGCGGGG TGGCAGCCAG GCCGGGCACC 
TTGCATGATT TCCACAGTCA TGAAATAGCC GAGCAGCTGA CACTCTTAGA TGCTGAACTC 
TTCTATAAAA TCGAGATACC AGAAGTTTTG CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG 
AGCCCCAACC TGACGCAGTT CACAGAGCAC TTTAATAACA TGTCCTACTG GGTCCGTTCC 
ATAATAATGC TTCAAGAGAA AGCCCAAGAC CGAGAGAGGC TGCTGCTTAA GTTCATAAAG 
ATTATGAAGC ACTTACGAAA GCTGAATAAT TTTAATTCCT ATCTGGCCAT TCTTTCTGCA 
CTGGATTCTG CACCCATCCG AAGGCTGGAG TGGCAGAAAC AAACCTCAGA GGGCCTGGCT 
GAGTACTGCA CACTGATCGA CAGCTCCTCG TCCTTCCGCG CCTACCGTGC AGCCCTGGCT 
GATGTGGAGC CTCCCTGCAT CCCATACCTA GGCCTAATTC TGCAGGACCT GACCTTTGTT 
CATCTGGGAA ACCCAGACTA CATCGACAGC AAGGTGAACT TCTCCAAGCG CTGGCAGCAG 
TTTAACATCC TGGACAGCAT GAGGTGCTTC CAACAGGTAC ATTACGACAT CAAGAGAAAC 
GACGACATCG TGTCGTTCTT CAACGACTTC AGCGACCATC TGGCGGAGGA GGCCCTGTGG 
GAACTGTCCC TCAAAATCAA ACCTCGGAAC ATTACAAGGC GGAAAACAGA CCGGGAAGAG 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279743.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135229.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OGa43665
Clone ID Related Accession (Same CDS sequence)	XM_015279743.1 , XM_015279743.2
Accession Version	XM_015279743.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC CGTGCCCAGA TTCCGAGCGC TCCCATCTCT CCTCGTTCAC TATGAAGCTG 
AAGGGCAAGT TTCATTCACC AAAAATAAAG AGAACTCCTT CCAAGAAGGG AAAGCAAGCT 
GACCTGACAG TGAAAACACC AGAGAAGTCA GCGAACAAAA ACTTATCATG GCTAGAGGAG 
AAGGAGAAGG AGGTCGTCAG TGCTCTGCGC TACTTCAAGA CGATTGTGGA CAAAATGGCA 
ATTGATAAGA AAGTATTAGA GATGCTGCCA GGCTCAGCAA GTAAAGTGCT GGAAGCCATC 
CTGCCCCTGG TGCAAAGTGA CCCACGCATC CAACAAAGTT CTGCCATCTC GTCCTGCTAC 
AGCCGTGTGT ACCAGAGCCT GGCCAATCTC ATTCGCTGGT CTGATCAAGT GATGCTGGAA 
GGTGTGAATT CTGAAGACAA AGAGATGGTG ACAACTGTTA AAGACGTCAT AAAAGCTGTT 
CTGGATGGAG TTAAGGAGCT GGTCAAACTC ACGATTGAAA AGCAGGAGCA TCCCTCTCCC 
ACAAGCCCTG TTAAACCCAG TTTACCAGCA TGTAAATCTG ATAGCCCATC AGAACTTCCC 
CTTACAGACC GAGAAATGGA GATCCTAAAC AAAACAACTA ATATGACTCA ATCAAACGAG 
CTACTGACAG ACTCCATGGA CGAAGAGGTT GCACCTCCTA AACCCCCCCT GCCTTGCATT 
CGTGTGGCAG AAAACAGCCC TCCTCCAGCC TTGCCCCCTA AAAAACGGCA GTCGGCGCCT 
TCCCCAACCA GAGTGGCAGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG GTCTAGTTTG 
CCTGTTGGAA TCAACAGACA GGATTTTGAT GTTGACTGCT ATGCCCAGAG AAGGCTGTCA 
GGTGGCAGCC ACTCCTACGG GGGAGAATCT CCTCGCCTCT CACCGTGCAG CAGCATTGGC 
AAACTCAGCA AGTCCGATGA GCAGCTCTCT TCTCTGGACC GTGACAGTGG TCAGTGCTCC 
CGCAACACAA GCTGTGAAAC ATTAGATCAA TCAGATCACT ATGACCCGGA CTATGAATTC 
CTGCAGCAGG ACCTCTCAAA TGCTGATCAG ATACCTCAAC AGGTGCCAAC TACCCTCAGC 
CCCTTGCCAG AGTCCCTGGG TGAGTCTGGC TCCCCTTTCC ACGGACATGC TTTCCAGCTC 
CCACAGGGCA GCTCTTCTCC ACTGGAATTC TGCAGCCTCT CGGGAGCTGT GCAGCCAACA 
GAGACCCCTC CAGCTTTACC AGAAAAGAAG AGGAGAAGCG CAGCCTCTCA AACTTCAGAC 
TCAAGCTGCA GGGTCTCATA CGAGAGACAC CCTTCACAAT ATGATAACAT CTCAGAGGAC 
GATATGCAGA ACGCAGGGTC TCTCTCATCA GTACCCTGCA CGCCATTTGC TCCTATTTTG 
CCCTTCCAAC AAGGAAACTC TTCAGCACAA GTTGAATTCA TTGCTGATTT TGCTGCTCCA 
GATTCTAACA GTGACCCAGA GAAGCCACCA CCTCTGCCAG AGAAGAAAAA CAAACACATG 
ATGGCGTACA TGCAGTTTGT AGAGGACTAC TCGGAGCCAC AGCCATCTAT GTTCTACCAG 
ACCCCTCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC ACCTCATGGA GGTCTACGGC 
TTCAACGACT CCTTTATCAG CTTAGACCCT TCTCAAGATC TGGCCCCCCC TCCTGCTCTC 
CCACCGAAGC AGCGACAGCT GCAGGCCTCC TATGCTGCCT CTTCTTTCCC TTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACCCCCGAGG ACACCGGTGC CGCTCAGGGC 
CTCACTATGT CAGTCTCTAA CTCCTTTCTC AACCGGCACA GCTCCTTTCC TGTGCATTCG 
GGCTGGAGCA AACGGCATCT TAAATTGGGA TCCAGTGGAA AATCCCAGCA AAGCAGTAAG 
CCCTCAGAGT GCTTTTCCCT GCCGAGATGC TTCCAGCACG CTCAGAGCCG TGGTTTCTGT 
ATGCCTTCGC ATCCCTTCAG CCAGCCTGGT GCTGTCCAGG TGTGCTCTGT TGCTCACAGC 
TGCAGCACCA GGCGTCCCTC CGTGCTGACC GCCCCGCTGC CACCATCCTG CCCTGAGCGA 
GGGCTGCTGT TTGTGGAAGG CCTCACCTCA CCACCAAACC TGCCCGCCCA GTACAAGTCT 
GTGTTTAGGT CCTACTCGCA GGACTTTGTG CCTCACAACC AGGTCTCCGT ACCTCCTTTC 
CTGTCTTCTA CCTCCTCCTC CTGCTCCACC GCGCCCTTTC CGCCCGTCCA CCCGTCTCCG 
AGCTCTGACC TCGCAGTGCC AGCCACGGCC AGCCCGTCTC CCAGCACTGG GGATGTGCCA 
AACTCCTCTT CGCAGGAGAG CAGCTTTAAC GGGAGTGCTC CTGTCTGCCT TCCTTCTGAA 
ACCTCTCTCA CTGATTCTCT GCAGACGCCT TCGAGTGAAA ACGCCAGTGA GGAGGCTGGT 
GAGGGTGAAT ACGTCAATCT GTATTCCTCT GGCCAGAGCA ACGGGGAACT CCCTCATTCT 
GGAGGAGAAT CTCCCTCTGT CAAAGACGGC CATTCCAAAG ACTCCACTTC AAACAGCAGT 
GCCATGGGGA AGGAAGGCAA AGATGGTGCT GAGAGGCAGC AGCAGTCGCC AGATGCTTTG 
GAATCGTCAC AGTTGGAGGA AGAAGTGGAT GAGCTCTCCC TTATTGACCA CAGTGAAATT 
ATGGCTAGGT TAACACTGAA GCAAGAGGGA GATGATGGGC CAGATGTCCG CGGTGGATCT 
GGAGATATTT TGTTGGTGCA TGCAACAGAG ACCGACAGGA AAGATCTGGT GCTGTACTGT 
GAAGCCTTCC TGACTACATA CAGGACATTT ATTACCCCTG AAGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAGTT TTGCCACTTC CCAGACACGT TTAAGAAGCG AGTCAGTAAG 
AACACCTTCT TCGTGCTGGT GAGAGTGGTG GATGAGCTGT GCTTAGTGGA GCTGACGGAG 
GAGATTCTCA AGCTGCTGAT GGACCTGGTG TTCCGGCTGG TCTGCAATGG GGAGCTGAGC 
CTCGCCAGAG TGCTGCGCAA AAACATCCTG GACAAAGTGG ATCAGAAGAA GATGTTGAGA 
TACGCCAACT CCATCAAACC TCTCGCAGCC CGCGGGGTGG CAGCCAGGCC GGGCACCTTG 
CATGATTTCC ACAGTCATGA AATAGCCGAG CAGCTGACAC TCTTAGATGC TGAACTCTTC 
TATAAAATCG AGATACCAGA AGTTTTGCTT TGGGCGAAAG AGCAAAATGA AGAGAAGAGC 
CCCAACCTGA CGCAGTTCAC AGAGCACTTT AATAACATGT CCTACTGGGT CCGTTCCATA 
ATAATGCTTC AAGAGAAAGC CCAAGACCGA GAGAGGCTGC TGCTTAAGTT CATAAAGATT 
ATGAAGCACT TACGAAAGCT GAATAATTTT AATTCCTATC TGGCCATTCT TTCTGCACTG 
GATTCTGCAC CCATCCGAAG GCTGGAGTGG CAGAAACAAA CCTCAGAGGG CCTGGCTGAG 
TACTGCACAC TGATCGACAG CTCCTCGTCC TTCCGCGCCT ACCGTGCAGC CCTGGCTGAT 
GTGGAGCCTC CCTGCATCCC ATACCTAGGC CTAATTCTGC AGGACCTGAC CTTTGTTCAT 
CTGGGAAACC CAGACTACAT CGACAGCAAG GTGAACTTCT CCAAGCGCTG GCAGCAGTTT 
AACATCCTGG ACAGCATGAG GTGCTTCCAA CAGGTACATT ACGACATCAA GAGAAACGAC 
GACATCGTGT CGTTCTTCAA CGACTTCAGC GACCATCTGG CGGAGGAGGC CCTGTGGGAA 
CTGTCCCTCA AAATCAAACC TCGGAACATT ACAAGGCGGA AAACAGACCG GGAAGAGAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135229.1
CDS	475..4440
Translation	MQDPCPDSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279743.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279743.1

ORF Insert Sequence:

ATGCAGGATC CGTGCCCAGA TTCCGAGCGC TCCCATCTCT CCTCGTTCAC TATGAAGCTG 
AAGGGCAAGT TTCATTCACC AAAAATAAAG AGAACTCCTT CCAAGAAGGG AAAGCAAGCT 
GACCTGACAG TGAAAACACC AGAGAAGTCA GCGAACAAAA ACTTATCATG GCTAGAGGAG 
AAGGAGAAGG AGGTCGTCAG TGCTCTGCGC TACTTCAAGA CGATTGTGGA CAAAATGGCA 
ATTGATAAGA AAGTATTAGA GATGCTGCCA GGCTCAGCAA GTAAAGTGCT GGAAGCCATC 
CTGCCCCTGG TGCAAAGTGA CCCACGCATC CAACAAAGTT CTGCCATCTC GTCCTGCTAC 
AGCCGTGTGT ACCAGAGCCT GGCCAATCTC ATTCGCTGGT CTGATCAAGT GATGCTGGAA 
GGTGTGAATT CTGAAGACAA AGAGATGGTG ACAACTGTTA AAGACGTCAT AAAAGCTGTT 
CTGGATGGAG TTAAGGAGCT GGTCAAACTC ACGATTGAAA AGCAGGAGCA TCCCTCTCCC 
ACAAGCCCTG TTAAACCCAG TTTACCAGCA TGTAAATCTG ATAGCCCATC AGAACTTCCC 
CTTACAGACC GAGAAATGGA GATCCTAAAC AAAACAACTA ATATGACTCA ATCAAACGAG 
CTACTGACAG ACTCCATGGA CGAAGAGGTT GCACCTCCTA AACCCCCCCT GCCTTGCATT 
CGTGTGGCAG AAAACAGCCC TCCTCCAGCC TTGCCCCCTA AAAAACGGCA GTCGGCGCCT 
TCCCCAACCA GAGTGGCAGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG GTCTAGTTTG 
CCTGTTGGAA TCAACAGACA GGATTTTGAT GTTGACTGCT ATGCCCAGAG AAGGCTGTCA 
GGTGGCAGCC ACTCCTACGG GGGAGAATCT CCTCGCCTCT CACCGTGCAG CAGCATTGGC 
AAACTCAGCA AGTCCGATGA GCAGCTCTCT TCTCTGGACC GTGACAGTGG TCAGTGCTCC 
CGCAACACAA GCTGTGAAAC ATTAGATCAA TCAGATCACT ATGACCCGGA CTATGAATTC 
CTGCAGCAGG ACCTCTCAAA TGCTGATCAG ATACCTCAAC AGGTGCCAAC TACCCTCAGC 
CCCTTGCCAG AGTCCCTGGG TGAGTCTGGC TCCCCTTTCC ACGGACATGC TTTCCAGCTC 
CCACAGGGCA GCTCTTCTCC ACTGGAATTC TGCAGCCTCT CGGGAGCTGT GCAGCCAACA 
GAGACCCCTC CAGCTTTACC AGAAAAGAAG AGGAGAAGCG CAGCCTCTCA AACTTCAGAC 
TCAAGCTGCA GGGTCTCATA CGAGAGACAC CCTTCACAAT ATGATAACAT CTCAGAGGAC 
GATATGCAGA ACGCAGGGTC TCTCTCATCA GTACCCTGCA CGCCATTTGC TCCTATTTTG 
CCCTTCCAAC AAGGAAACTC TTCAGCACAA GTTGAATTCA TTGCTGATTT TGCTGCTCCA 
GATTCTAACA GTGACCCAGA GAAGCCACCA CCTCTGCCAG AGAAGAAAAA CAAACACATG 
ATGGCGTACA TGCAGTTTGT AGAGGACTAC TCGGAGCCAC AGCCATCTAT GTTCTACCAG 
ACCCCTCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC ACCTCATGGA GGTCTACGGC 
TTCAACGACT CCTTTATCAG CTTAGACCCT TCTCAAGATC TGGCCCCCCC TCCTGCTCTC 
CCACCGAAGC AGCGACAGCT GCAGGCCTCC TATGCTGCCT CTTCTTTCCC TTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACCCCCGAGG ACACCGGTGC CGCTCAGGGC 
CTCACTATGT CAGTCTCTAA CTCCTTTCTC AACCGGCACA GCTCCTTTCC TGTGCATTCG 
GGCTGGAGCA AACGGCATCT TAAATTGGGA TCCAGTGGAA AATCCCAGCA AAGCAGTAAG 
CCCTCAGAGT GCTTTTCCCT GCCGAGATGC TTCCAGCACG CTCAGAGCCG TGGTTTCTGT 
ATGCCTTCGC ATCCCTTCAG CCAGCCTGGT GCTGTCCAGG TGTGCTCTGT TGCTCACAGC 
TGCAGCACCA GGCGTCCCTC CGTGCTGACC GCCCCGCTGC CACCATCCTG CCCTGAGCGA 
GGGCTGCTGT TTGTGGAAGG CCTCACCTCA CCACCAAACC TGCCCGCCCA GTACAAGTCT 
GTGTTTAGGT CCTACTCGCA GGACTTTGTG CCTCACAACC AGGTCTCCGT ACCTCCTTTC 
CTGTCTTCTA CCTCCTCCTC CTGCTCCACC GCGCCCTTTC CGCCCGTCCA CCCGTCTCCG 
AGCTCTGACC TCGCAGTGCC AGCCACGGCC AGCCCGTCTC CCAGCACTGG GGATGTGCCA 
AACTCCTCTT CGCAGGAGAG CAGCTTTAAC GGGAGTGCTC CTGTCTGCCT TCCTTCTGAA 
ACCTCTCTCA CTGATTCTCT GCAGACGCCT TCGAGTGAAA ACGCCAGTGA GGAGGCTGGT 
GAGGGTGAAT ACGTCAATCT GTATTCCTCT GGCCAGAGCA ACGGGGAACT CCCTCATTCT 
GGAGGAGAAT CTCCCTCTGT CAAAGACGGC CATTCCAAAG ACTCCACTTC AAACAGCAGT 
GCCATGGGGA AGGAAGGCAA AGATGGTGCT GAGAGGCAGC AGCAGTCGCC AGATGCTTTG 
GAATCGTCAC AGTTGGAGGA AGAAGTGGAT GAGCTCTCCC TTATTGACCA CAGTGAAATT 
ATGGCTAGGT TAACACTGAA GCAAGAGGGA GATGATGGGC CAGATGTCCG CGGTGGATCT 
GGAGATATTT TGTTGGTGCA TGCAACAGAG ACCGACAGGA AAGATCTGGT GCTGTACTGT 
GAAGCCTTCC TGACTACATA CAGGACATTT ATTACCCCTG AAGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAGTT TTGCCACTTC CCAGACACGT TTAAGAAGCG AGTCAGTAAG 
AACACCTTCT TCGTGCTGGT GAGAGTGGTG GATGAGCTGT GCTTAGTGGA GCTGACGGAG 
GAGATTCTCA AGCTGCTGAT GGACCTGGTG TTCCGGCTGG TCTGCAATGG GGAGCTGAGC 
CTCGCCAGAG TGCTGCGCAA AAACATCCTG GACAAAGTGG ATCAGAAGAA GATGTTGAGA 
TACGCCAACT CCATCAAACC TCTCGCAGCC CGCGGGGTGG CAGCCAGGCC GGGCACCTTG 
CATGATTTCC ACAGTCATGA AATAGCCGAG CAGCTGACAC TCTTAGATGC TGAACTCTTC 
TATAAAATCG AGATACCAGA AGTTTTGCTT TGGGCGAAAG AGCAAAATGA AGAGAAGAGC 
CCCAACCTGA CGCAGTTCAC AGAGCACTTT AATAACATGT CCTACTGGGT CCGTTCCATA 
ATAATGCTTC AAGAGAAAGC CCAAGACCGA GAGAGGCTGC TGCTTAAGTT CATAAAGATT 
ATGAAGCACT TACGAAAGCT GAATAATTTT AATTCCTATC TGGCCATTCT TTCTGCACTG 
GATTCTGCAC CCATCCGAAG GCTGGAGTGG CAGAAACAAA CCTCAGAGGG CCTGGCTGAG 
TACTGCACAC TGATCGACAG CTCCTCGTCC TTCCGCGCCT ACCGTGCAGC CCTGGCTGAT 
GTGGAGCCTC CCTGCATCCC ATACCTAGGC CTAATTCTGC AGGACCTGAC CTTTGTTCAT 
CTGGGAAACC CAGACTACAT CGACAGCAAG GTGAACTTCT CCAAGCGCTG GCAGCAGTTT 
AACATCCTGG ACAGCATGAG GTGCTTCCAA CAGGTACATT ACGACATCAA GAGAAACGAC 
GACATCGTGT CGTTCTTCAA CGACTTCAGC GACCATCTGG CGGAGGAGGC CCTGTGGGAA 
CTGTCCCTCA AAATCAAACC TCGGAACATT ACAAGGCGGA AAACAGACCG GGAAGAGAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279743.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135229.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa43665
Clone ID Related Accession (Same CDS sequence)	XM_015279743.1 , XM_015279743.2
Accession Version	XM_015279743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC CGTGCCCAGA TTCCGAGCGC TCCCATCTCT CCTCGTTCAC TATGAAGCTG 
AAGGGCAAGT TTCATTCACC AAAAATAAAG AGAACTCCTT CCAAGAAGGG AAAGCAAGCT 
GACCTGACAG TGAAAACACC AGAGAAGTCA GCGAACAAAA ACTTATCATG GCTAGAGGAG 
AAGGAGAAGG AGGTCGTCAG TGCTCTGCGC TACTTCAAGA CGATTGTGGA CAAAATGGCA 
ATTGATAAGA AAGTATTAGA GATGCTGCCA GGCTCAGCAA GTAAAGTGCT GGAAGCCATC 
CTGCCCCTGG TGCAAAGTGA CCCACGCATC CAACAAAGTT CTGCCATCTC GTCCTGCTAC 
AGCCGTGTGT ACCAGAGCCT GGCCAATCTC ATTCGCTGGT CTGATCAAGT GATGCTGGAA 
GGTGTGAATT CTGAAGACAA AGAGATGGTG ACAACTGTTA AAGACGTCAT AAAAGCTGTT 
CTGGATGGAG TTAAGGAGCT GGTCAAACTC ACGATTGAAA AGCAGGAGCA TCCCTCTCCC 
ACAAGCCCTG TTAAACCCAG TTTACCAGCA TGTAAATCTG ATAGCCCATC AGAACTTCCC 
CTTACAGACC GAGAAATGGA GATCCTAAAC AAAACAACTA ATATGACTCA ATCAAACGAG 
CTACTGACAG ACTCCATGGA CGAAGAGGTT GCACCTCCTA AACCCCCCCT GCCTTGCATT 
CGTGTGGCAG AAAACAGCCC TCCTCCAGCC TTGCCCCCTA AAAAACGGCA GTCGGCGCCT 
TCCCCAACCA GAGTGGCAGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG GTCTAGTTTG 
CCTGTTGGAA TCAACAGACA GGATTTTGAT GTTGACTGCT ATGCCCAGAG AAGGCTGTCA 
GGTGGCAGCC ACTCCTACGG GGGAGAATCT CCTCGCCTCT CACCGTGCAG CAGCATTGGC 
AAACTCAGCA AGTCCGATGA GCAGCTCTCT TCTCTGGACC GTGACAGTGG TCAGTGCTCC 
CGCAACACAA GCTGTGAAAC ATTAGATCAA TCAGATCACT ATGACCCGGA CTATGAATTC 
CTGCAGCAGG ACCTCTCAAA TGCTGATCAG ATACCTCAAC AGGTGCCAAC TACCCTCAGC 
CCCTTGCCAG AGTCCCTGGG TGAGTCTGGC TCCCCTTTCC ACGGACATGC TTTCCAGCTC 
CCACAGGGCA GCTCTTCTCC ACTGGAATTC TGCAGCCTCT CGGGAGCTGT GCAGCCAACA 
GAGACCCCTC CAGCTTTACC AGAAAAGAAG AGGAGAAGCG CAGCCTCTCA AACTTCAGAC 
TCAAGCTGCA GGGTCTCATA CGAGAGACAC CCTTCACAAT ATGATAACAT CTCAGAGGAC 
GATATGCAGA ACGCAGGGTC TCTCTCATCA GTACCCTGCA CGCCATTTGC TCCTATTTTG 
CCCTTCCAAC AAGGAAACTC TTCAGCACAA GTTGAATTCA TTGCTGATTT TGCTGCTCCA 
GATTCTAACA GTGACCCAGA GAAGCCACCA CCTCTGCCAG AGAAGAAAAA CAAACACATG 
ATGGCGTACA TGCAGTTTGT AGAGGACTAC TCGGAGCCAC AGCCATCTAT GTTCTACCAG 
ACCCCTCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC ACCTCATGGA GGTCTACGGC 
TTCAACGACT CCTTTATCAG CTTAGACCCT TCTCAAGATC TGGCCCCCCC TCCTGCTCTC 
CCACCGAAGC AGCGACAGCT GCAGGCCTCC TATGCTGCCT CTTCTTTCCC TTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACCCCCGAGG ACACCGGTGC CGCTCAGGGC 
CTCACTATGT CAGTCTCTAA CTCCTTTCTC AACCGGCACA GCTCCTTTCC TGTGCATTCG 
GGCTGGAGCA AACGGCATCT TAAATTGGGA TCCAGTGGAA AATCCCAGCA AAGCAGTAAG 
CCCTCAGAGT GCTTTTCCCT GCCGAGATGC TTCCAGCACG CTCAGAGCCG TGGTTTCTGT 
ATGCCTTCGC ATCCCTTCAG CCAGCCTGGT GCTGTCCAGG TGTGCTCTGT TGCTCACAGC 
TGCAGCACCA GGCGTCCCTC CGTGCTGACC GCCCCGCTGC CACCATCCTG CCCTGAGCGA 
GGGCTGCTGT TTGTGGAAGG CCTCACCTCA CCACCAAACC TGCCCGCCCA GTACAAGTCT 
GTGTTTAGGT CCTACTCGCA GGACTTTGTG CCTCACAACC AGGTCTCCGT ACCTCCTTTC 
CTGTCTTCTA CCTCCTCCTC CTGCTCCACC GCGCCCTTTC CGCCCGTCCA CCCGTCTCCG 
AGCTCTGACC TCGCAGTGCC AGCCACGGCC AGCCCGTCTC CCAGCACTGG GGATGTGCCA 
AACTCCTCTT CGCAGGAGAG CAGCTTTAAC GGGAGTGCTC CTGTCTGCCT TCCTTCTGAA 
ACCTCTCTCA CTGATTCTCT GCAGACGCCT TCGAGTGAAA ACGCCAGTGA GGAGGCTGGT 
GAGGGTGAAT ACGTCAATCT GTATTCCTCT GGCCAGAGCA ACGGGGAACT CCCTCATTCT 
GGAGGAGAAT CTCCCTCTGT CAAAGACGGC CATTCCAAAG ACTCCACTTC AAACAGCAGT 
GCCATGGGGA AGGAAGGCAA AGATGGTGCT GAGAGGCAGC AGCAGTCGCC AGATGCTTTG 
GAATCGTCAC AGTTGGAGGA AGAAGTGGAT GAGCTCTCCC TTATTGACCA CAGTGAAATT 
ATGGCTAGGT TAACACTGAA GCAAGAGGGA GATGATGGGC CAGATGTCCG CGGTGGATCT 
GGAGATATTT TGTTGGTGCA TGCAACAGAG ACCGACAGGA AAGATCTGGT GCTGTACTGT 
GAAGCCTTCC TGACTACATA CAGGACATTT ATTACCCCTG AAGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAGTT TTGCCACTTC CCAGACACGT TTAAGAAGCG AGTCAGTAAG 
AACACCTTCT TCGTGCTGGT GAGAGTGGTG GATGAGCTGT GCTTAGTGGA GCTGACGGAG 
GAGATTCTCA AGCTGCTGAT GGACCTGGTG TTCCGGCTGG TCTGCAATGG GGAGCTGAGC 
CTCGCCAGAG TGCTGCGCAA AAACATCCTG GACAAAGTGG ATCAGAAGAA GATGTTGAGA 
TACGCCAACT CCATCAAACC TCTCGCAGCC CGCGGGGTGG CAGCCAGGCC GGGCACCTTG 
CATGATTTCC ACAGTCATGA AATAGCCGAG CAGCTGACAC TCTTAGATGC TGAACTCTTC 
TATAAAATCG AGATACCAGA AGTTTTGCTT TGGGCGAAAG AGCAAAATGA AGAGAAGAGC 
CCCAACCTGA CGCAGTTCAC AGAGCACTTT AATAACATGT CCTACTGGGT CCGTTCCATA 
ATAATGCTTC AAGAGAAAGC CCAAGACCGA GAGAGGCTGC TGCTTAAGTT CATAAAGATT 
ATGAAGCACT TACGAAAGCT GAATAATTTT AATTCCTATC TGGCCATTCT TTCTGCACTG 
GATTCTGCAC CCATCCGAAG GCTGGAGTGG CAGAAACAAA CCTCAGAGGG CCTGGCTGAG 
TACTGCACAC TGATCGACAG CTCCTCGTCC TTCCGCGCCT ACCGTGCAGC CCTGGCTGAT 
GTGGAGCCTC CCTGCATCCC ATACCTAGGC CTAATTCTGC AGGACCTGAC CTTTGTTCAT 
CTGGGAAACC CAGACTACAT CGACAGCAAG GTGAACTTCT CCAAGCGCTG GCAGCAGTTT 
AACATCCTGG ACAGCATGAG GTGCTTCCAA CAGGTACATT ACGACATCAA GAGAAACGAC 
GACATCGTGT CGTTCTTCAA CGACTTCAGC GACCATCTGG CGGAGGAGGC CCTGTGGGAA 
CTGTCCCTCA AAATCAAACC TCGGAACATT ACAAGGCGGA AAACAGACCG GGAAGAGAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135229.1
CDS	491..4456
Translation	MQDPCPDSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279743.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279743.2

ORF Insert Sequence:

ATGCAGGATC CGTGCCCAGA TTCCGAGCGC TCCCATCTCT CCTCGTTCAC TATGAAGCTG 
AAGGGCAAGT TTCATTCACC AAAAATAAAG AGAACTCCTT CCAAGAAGGG AAAGCAAGCT 
GACCTGACAG TGAAAACACC AGAGAAGTCA GCGAACAAAA ACTTATCATG GCTAGAGGAG 
AAGGAGAAGG AGGTCGTCAG TGCTCTGCGC TACTTCAAGA CGATTGTGGA CAAAATGGCA 
ATTGATAAGA AAGTATTAGA GATGCTGCCA GGCTCAGCAA GTAAAGTGCT GGAAGCCATC 
CTGCCCCTGG TGCAAAGTGA CCCACGCATC CAACAAAGTT CTGCCATCTC GTCCTGCTAC 
AGCCGTGTGT ACCAGAGCCT GGCCAATCTC ATTCGCTGGT CTGATCAAGT GATGCTGGAA 
GGTGTGAATT CTGAAGACAA AGAGATGGTG ACAACTGTTA AAGACGTCAT AAAAGCTGTT 
CTGGATGGAG TTAAGGAGCT GGTCAAACTC ACGATTGAAA AGCAGGAGCA TCCCTCTCCC 
ACAAGCCCTG TTAAACCCAG TTTACCAGCA TGTAAATCTG ATAGCCCATC AGAACTTCCC 
CTTACAGACC GAGAAATGGA GATCCTAAAC AAAACAACTA ATATGACTCA ATCAAACGAG 
CTACTGACAG ACTCCATGGA CGAAGAGGTT GCACCTCCTA AACCCCCCCT GCCTTGCATT 
CGTGTGGCAG AAAACAGCCC TCCTCCAGCC TTGCCCCCTA AAAAACGGCA GTCGGCGCCT 
TCCCCAACCA GAGTGGCAGT TGTGGCCCCC ATGAGTCGAG CCACCAGTGG GTCTAGTTTG 
CCTGTTGGAA TCAACAGACA GGATTTTGAT GTTGACTGCT ATGCCCAGAG AAGGCTGTCA 
GGTGGCAGCC ACTCCTACGG GGGAGAATCT CCTCGCCTCT CACCGTGCAG CAGCATTGGC 
AAACTCAGCA AGTCCGATGA GCAGCTCTCT TCTCTGGACC GTGACAGTGG TCAGTGCTCC 
CGCAACACAA GCTGTGAAAC ATTAGATCAA TCAGATCACT ATGACCCGGA CTATGAATTC 
CTGCAGCAGG ACCTCTCAAA TGCTGATCAG ATACCTCAAC AGGTGCCAAC TACCCTCAGC 
CCCTTGCCAG AGTCCCTGGG TGAGTCTGGC TCCCCTTTCC ACGGACATGC TTTCCAGCTC 
CCACAGGGCA GCTCTTCTCC ACTGGAATTC TGCAGCCTCT CGGGAGCTGT GCAGCCAACA 
GAGACCCCTC CAGCTTTACC AGAAAAGAAG AGGAGAAGCG CAGCCTCTCA AACTTCAGAC 
TCAAGCTGCA GGGTCTCATA CGAGAGACAC CCTTCACAAT ATGATAACAT CTCAGAGGAC 
GATATGCAGA ACGCAGGGTC TCTCTCATCA GTACCCTGCA CGCCATTTGC TCCTATTTTG 
CCCTTCCAAC AAGGAAACTC TTCAGCACAA GTTGAATTCA TTGCTGATTT TGCTGCTCCA 
GATTCTAACA GTGACCCAGA GAAGCCACCA CCTCTGCCAG AGAAGAAAAA CAAACACATG 
ATGGCGTACA TGCAGTTTGT AGAGGACTAC TCGGAGCCAC AGCCATCTAT GTTCTACCAG 
ACCCCTCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC ACCTCATGGA GGTCTACGGC 
TTCAACGACT CCTTTATCAG CTTAGACCCT TCTCAAGATC TGGCCCCCCC TCCTGCTCTC 
CCACCGAAGC AGCGACAGCT GCAGGCCTCC TATGCTGCCT CTTCTTTCCC TTCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACCCCCGAGG ACACCGGTGC CGCTCAGGGC 
CTCACTATGT CAGTCTCTAA CTCCTTTCTC AACCGGCACA GCTCCTTTCC TGTGCATTCG 
GGCTGGAGCA AACGGCATCT TAAATTGGGA TCCAGTGGAA AATCCCAGCA AAGCAGTAAG 
CCCTCAGAGT GCTTTTCCCT GCCGAGATGC TTCCAGCACG CTCAGAGCCG TGGTTTCTGT 
ATGCCTTCGC ATCCCTTCAG CCAGCCTGGT GCTGTCCAGG TGTGCTCTGT TGCTCACAGC 
TGCAGCACCA GGCGTCCCTC CGTGCTGACC GCCCCGCTGC CACCATCCTG CCCTGAGCGA 
GGGCTGCTGT TTGTGGAAGG CCTCACCTCA CCACCAAACC TGCCCGCCCA GTACAAGTCT 
GTGTTTAGGT CCTACTCGCA GGACTTTGTG CCTCACAACC AGGTCTCCGT ACCTCCTTTC 
CTGTCTTCTA CCTCCTCCTC CTGCTCCACC GCGCCCTTTC CGCCCGTCCA CCCGTCTCCG 
AGCTCTGACC TCGCAGTGCC AGCCACGGCC AGCCCGTCTC CCAGCACTGG GGATGTGCCA 
AACTCCTCTT CGCAGGAGAG CAGCTTTAAC GGGAGTGCTC CTGTCTGCCT TCCTTCTGAA 
ACCTCTCTCA CTGATTCTCT GCAGACGCCT TCGAGTGAAA ACGCCAGTGA GGAGGCTGGT 
GAGGGTGAAT ACGTCAATCT GTATTCCTCT GGCCAGAGCA ACGGGGAACT CCCTCATTCT 
GGAGGAGAAT CTCCCTCTGT CAAAGACGGC CATTCCAAAG ACTCCACTTC AAACAGCAGT 
GCCATGGGGA AGGAAGGCAA AGATGGTGCT GAGAGGCAGC AGCAGTCGCC AGATGCTTTG 
GAATCGTCAC AGTTGGAGGA AGAAGTGGAT GAGCTCTCCC TTATTGACCA CAGTGAAATT 
ATGGCTAGGT TAACACTGAA GCAAGAGGGA GATGATGGGC CAGATGTCCG CGGTGGATCT 
GGAGATATTT TGTTGGTGCA TGCAACAGAG ACCGACAGGA AAGATCTGGT GCTGTACTGT 
GAAGCCTTCC TGACTACATA CAGGACATTT ATTACCCCTG AAGAGCTCAT CAAGAAGCTG 
CAGTACAGAT ATGAGAAGTT TTGCCACTTC CCAGACACGT TTAAGAAGCG AGTCAGTAAG 
AACACCTTCT TCGTGCTGGT GAGAGTGGTG GATGAGCTGT GCTTAGTGGA GCTGACGGAG 
GAGATTCTCA AGCTGCTGAT GGACCTGGTG TTCCGGCTGG TCTGCAATGG GGAGCTGAGC 
CTCGCCAGAG TGCTGCGCAA AAACATCCTG GACAAAGTGG ATCAGAAGAA GATGTTGAGA 
TACGCCAACT CCATCAAACC TCTCGCAGCC CGCGGGGTGG CAGCCAGGCC GGGCACCTTG 
CATGATTTCC ACAGTCATGA AATAGCCGAG CAGCTGACAC TCTTAGATGC TGAACTCTTC 
TATAAAATCG AGATACCAGA AGTTTTGCTT TGGGCGAAAG AGCAAAATGA AGAGAAGAGC 
CCCAACCTGA CGCAGTTCAC AGAGCACTTT AATAACATGT CCTACTGGGT CCGTTCCATA 
ATAATGCTTC AAGAGAAAGC CCAAGACCGA GAGAGGCTGC TGCTTAAGTT CATAAAGATT 
ATGAAGCACT TACGAAAGCT GAATAATTTT AATTCCTATC TGGCCATTCT TTCTGCACTG 
GATTCTGCAC CCATCCGAAG GCTGGAGTGG CAGAAACAAA CCTCAGAGGG CCTGGCTGAG 
TACTGCACAC TGATCGACAG CTCCTCGTCC TTCCGCGCCT ACCGTGCAGC CCTGGCTGAT 
GTGGAGCCTC CCTGCATCCC ATACCTAGGC CTAATTCTGC AGGACCTGAC CTTTGTTCAT 
CTGGGAAACC CAGACTACAT CGACAGCAAG GTGAACTTCT CCAAGCGCTG GCAGCAGTTT 
AACATCCTGG ACAGCATGAG GTGCTTCCAA CAGGTACATT ACGACATCAA GAGAAACGAC 
GACATCGTGT CGTTCTTCAA CGACTTCAGC GACCATCTGG CGGAGGAGGC CCTGTGGGAA 
CTGTCCCTCA AAATCAAACC TCGGAACATT ACAAGGCGGA AAACAGACCG GGAAGAGAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279744.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135230.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.

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CloneID	OGa43666
Clone ID Related Accession (Same CDS sequence)	XM_015279744.1 , XM_015279744.2
Accession Version	XM_015279744.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGGCAAGTT TCATTCACCA AAAATAAAGA GAACTCCTTC CAAGAAGGGA 
AAGCAAGCTG ACCTGACAGT GAAAACACCA GAGAAGTCAG CGAACAAAAA CTTATCATGG 
CTAGAGGAGA AGGAGAAGGA GGTCGTCAGT GCTCTGCGCT ACTTCAAGAC GATTGTGGAC 
AAAATGGCAA TTGATAAGAA AGTATTAGAG ATGCTGCCAG GCTCAGCAAG TAAAGTGCTG 
GAAGCCATCC TGCCCCTGGT GCAAAGTGAC CCACGCATCC AACAAAGTTC TGCCATCTCG 
TCCTGCTACA GCCGTGTGTA CCAGAGCCTG GCCAATCTCA TTCGCTGGTC TGATCAAGTG 
ATGCTGGAAG GTGTGAATTC TGAAGACAAA GAGATGGTGA CAACTGTTAA AGACGTCATA 
AAAGCTGTTC TGGATGGAGT TAAGGAGCTG GTCAAACTCA CGATTGAAAA GCAGGAGCAT 
CCCTCTCCCA CAAGCCCTGT TAAACCCAGT TTACCAGCAT GTAAATCTGA TAGCCCATCA 
GAACTTCCCC TTACAGACCG AGAAATGGAG ATCCTAAACA AAACAACTAA TATGACTCAA 
TCAAACGAGC TACTGACAGA CTCCATGGAC GAAGAGGTTG CACCTCCTAA ACCCCCCCTG 
CCTTGCATTC GTGTGGCAGA AAACAGCCCT CCTCCAGCCT TGCCCCCTAA AAAACGGCAG 
TCGGCGCCTT CCCCAACCAG AGTGGCAGTT GTGGCCCCCA TGAGTCGAGC CACCAGTGGG 
TCTAGTTTGC CTGTTGGAAT CAACAGACAG GATTTTGATG TTGACTGCTA TGCCCAGAGA 
AGGCTGTCAG GTGGCAGCCA CTCCTACGGG GGAGAATCTC CTCGCCTCTC ACCGTGCAGC 
AGCATTGGCA AACTCAGCAA GTCCGATGAG CAGCTCTCTT CTCTGGACCG TGACAGTGGT 
CAGTGCTCCC GCAACACAAG CTGTGAAACA TTAGATCAAT CAGATCACTA TGACCCGGAC 
TATGAATTCC TGCAGCAGGA CCTCTCAAAT GCTGATCAGA TACCTCAACA GGTGCCAACT 
ACCCTCAGCC CCTTGCCAGA GTCCCTGGGT GAGTCTGGCT CCCCTTTCCA CGGACATGCT 
TTCCAGCTCC CACAGGGCAG CTCTTCTCCA CTGGAATTCT GCAGCCTCTC GGGAGCTGTG 
CAGCCAACAG AGACCCCTCC AGCTTTACCA GAAAAGAAGA GGAGAAGCGC AGCCTCTCAA 
ACTTCAGACT CAAGCTGCAG GGTCTCATAC GAGAGACACC CTTCACAATA TGATAACATC 
TCAGAGGACG ATATGCAGAA CGCAGGGTCT CTCTCATCAG TACCCTGCAC GCCATTTGCT 
CCTATTTTGC CCTTCCAACA AGGAAACTCT TCAGCACAAG TTGAATTCAT TGCTGATTTT 
GCTGCTCCAG ATTCTAACAG TGACCCAGAG AAGCCACCAC CTCTGCCAGA GAAGAAAAAC 
AAACACATGA TGGCGTACAT GCAGTTTGTA GAGGACTACT CGGAGCCACA GCCATCTATG 
TTCTACCAGA CCCCTCAGAA CGAGCACATC TACCAGCAGA AGAACAAGCA CCTCATGGAG 
GTCTACGGCT TCAACGACTC CTTTATCAGC TTAGACCCTT CTCAAGATCT GGCCCCCCCT 
CCTGCTCTCC CACCGAAGCA GCGACAGCTG CAGGCCTCCT ATGCTGCCTC TTCTTTCCCT 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGCCCTTCA CCCCCGAGGA CACCGGTGCC 
GCTCAGGGCC TCACTATGTC AGTCTCTAAC TCCTTTCTCA ACCGGCACAG CTCCTTTCCT 
GTGCATTCGG GCTGGAGCAA ACGGCATCTT AAATTGGGAT CCAGTGGAAA ATCCCAGCAA 
AGCAGTAAGC CCTCAGAGTG CTTTTCCCTG CCGAGATGCT TCCAGCACGC TCAGAGCCGT 
GGTTTCTGTA TGCCTTCGCA TCCCTTCAGC CAGCCTGGTG CTGTCCAGGT GTGCTCTGTT 
GCTCACAGCT GCAGCACCAG GCGTCCCTCC GTGCTGACCG CCCCGCTGCC ACCATCCTGC 
CCTGAGCGAG GGCTGCTGTT TGTGGAAGGC CTCACCTCAC CACCAAACCT GCCCGCCCAG 
TACAAGTCTG TGTTTAGGTC CTACTCGCAG GACTTTGTGC CTCACAACCA GGTCTCCGTA 
CCTCCTTTCC TGTCTTCTAC CTCCTCCTCC TGCTCCACCG CGCCCTTTCC GCCCGTCCAC 
CCGTCTCCGA GCTCTGACCT CGCAGTGCCA GCCACGGCCA GCCCGTCTCC CAGCACTGGG 
GATGTGCCAA ACTCCTCTTC GCAGGAGAGC AGCTTTAACG GGAGTGCTCC TGTCTGCCTT 
CCTTCTGAAA CCTCTCTCAC TGATTCTCTG CAGACGCCTT CGAGTGAAAA CGCCAGTGAG 
GAGGCTGGTG AGGGTGAATA CGTCAATCTG TATTCCTCTG GCCAGAGCAA CGGGGAACTC 
CCTCATTCTG GAGGAGAATC TCCCTCTGTC AAAGACGGCC ATTCCAAAGA CTCCACTTCA 
AACAGCAGTG CCATGGGGAA GGAAGGCAAA GATGGTGCTG AGAGGCAGCA GCAGTCGCCA 
GATGCTTTGG AATCGTCACA GTTGGAGGAA GAAGTGGATG AGCTCTCCCT TATTGACCAC 
AGTGAAATTA TGGCTAGGTT AACACTGAAG CAAGAGGGAG ATGATGGGCC AGATGTCCGC 
GGTGGATCTG GAGATATTTT GTTGGTGCAT GCAACAGAGA CCGACAGGAA AGATCTGGTG 
CTGTACTGTG AAGCCTTCCT GACTACATAC AGGACATTTA TTACCCCTGA AGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAGTTT TGCCACTTCC CAGACACGTT TAAGAAGCGA 
GTCAGTAAGA ACACCTTCTT CGTGCTGGTG AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG 
CTGACGGAGG AGATTCTCAA GCTGCTGATG GACCTGGTGT TCCGGCTGGT CTGCAATGGG 
GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA AACATCCTGG ACAAAGTGGA TCAGAAGAAG 
ATGTTGAGAT ACGCCAACTC CATCAAACCT CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG 
GGCACCTTGC ATGATTTCCA CAGTCATGAA ATAGCCGAGC AGCTGACACT CTTAGATGCT 
GAACTCTTCT ATAAAATCGA GATACCAGAA GTTTTGCTTT GGGCGAAAGA GCAAAATGAA 
GAGAAGAGCC CCAACCTGAC GCAGTTCACA GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGTTCCATAA TAATGCTTCA AGAGAAAGCC CAAGACCGAG AGAGGCTGCT GCTTAAGTTC 
ATAAAGATTA TGAAGCACTT ACGAAAGCTG AATAATTTTA ATTCCTATCT GGCCATTCTT 
TCTGCACTGG ATTCTGCACC CATCCGAAGG CTGGAGTGGC AGAAACAAAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACACT GATCGACAGC TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC 
CTGGCTGATG TGGAGCCTCC CTGCATCCCA TACCTAGGCC TAATTCTGCA GGACCTGACC 
TTTGTTCATC TGGGAAACCC AGACTACATC GACAGCAAGG TGAACTTCTC CAAGCGCTGG 
CAGCAGTTTA ACATCCTGGA CAGCATGAGG TGCTTCCAAC AGGTACATTA CGACATCAAG 
AGAAACGACG ACATCGTGTC GTTCTTCAAC GACTTCAGCG ACCATCTGGC GGAGGAGGCC 
CTGTGGGAAC TGTCCCTCAA AATCAAACCT CGGAACATTA CAAGGCGGAA AACAGACCGG 
GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135230.1
CDS	57..3971
Translation	MKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279744.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279744.1

ORF Insert Sequence:

ATGAAGCTGA AGGGCAAGTT TCATTCACCA AAAATAAAGA GAACTCCTTC CAAGAAGGGA 
AAGCAAGCTG ACCTGACAGT GAAAACACCA GAGAAGTCAG CGAACAAAAA CTTATCATGG 
CTAGAGGAGA AGGAGAAGGA GGTCGTCAGT GCTCTGCGCT ACTTCAAGAC GATTGTGGAC 
AAAATGGCAA TTGATAAGAA AGTATTAGAG ATGCTGCCAG GCTCAGCAAG TAAAGTGCTG 
GAAGCCATCC TGCCCCTGGT GCAAAGTGAC CCACGCATCC AACAAAGTTC TGCCATCTCG 
TCCTGCTACA GCCGTGTGTA CCAGAGCCTG GCCAATCTCA TTCGCTGGTC TGATCAAGTG 
ATGCTGGAAG GTGTGAATTC TGAAGACAAA GAGATGGTGA CAACTGTTAA AGACGTCATA 
AAAGCTGTTC TGGATGGAGT TAAGGAGCTG GTCAAACTCA CGATTGAAAA GCAGGAGCAT 
CCCTCTCCCA CAAGCCCTGT TAAACCCAGT TTACCAGCAT GTAAATCTGA TAGCCCATCA 
GAACTTCCCC TTACAGACCG AGAAATGGAG ATCCTAAACA AAACAACTAA TATGACTCAA 
TCAAACGAGC TACTGACAGA CTCCATGGAC GAAGAGGTTG CACCTCCTAA ACCCCCCCTG 
CCTTGCATTC GTGTGGCAGA AAACAGCCCT CCTCCAGCCT TGCCCCCTAA AAAACGGCAG 
TCGGCGCCTT CCCCAACCAG AGTGGCAGTT GTGGCCCCCA TGAGTCGAGC CACCAGTGGG 
TCTAGTTTGC CTGTTGGAAT CAACAGACAG GATTTTGATG TTGACTGCTA TGCCCAGAGA 
AGGCTGTCAG GTGGCAGCCA CTCCTACGGG GGAGAATCTC CTCGCCTCTC ACCGTGCAGC 
AGCATTGGCA AACTCAGCAA GTCCGATGAG CAGCTCTCTT CTCTGGACCG TGACAGTGGT 
CAGTGCTCCC GCAACACAAG CTGTGAAACA TTAGATCAAT CAGATCACTA TGACCCGGAC 
TATGAATTCC TGCAGCAGGA CCTCTCAAAT GCTGATCAGA TACCTCAACA GGTGCCAACT 
ACCCTCAGCC CCTTGCCAGA GTCCCTGGGT GAGTCTGGCT CCCCTTTCCA CGGACATGCT 
TTCCAGCTCC CACAGGGCAG CTCTTCTCCA CTGGAATTCT GCAGCCTCTC GGGAGCTGTG 
CAGCCAACAG AGACCCCTCC AGCTTTACCA GAAAAGAAGA GGAGAAGCGC AGCCTCTCAA 
ACTTCAGACT CAAGCTGCAG GGTCTCATAC GAGAGACACC CTTCACAATA TGATAACATC 
TCAGAGGACG ATATGCAGAA CGCAGGGTCT CTCTCATCAG TACCCTGCAC GCCATTTGCT 
CCTATTTTGC CCTTCCAACA AGGAAACTCT TCAGCACAAG TTGAATTCAT TGCTGATTTT 
GCTGCTCCAG ATTCTAACAG TGACCCAGAG AAGCCACCAC CTCTGCCAGA GAAGAAAAAC 
AAACACATGA TGGCGTACAT GCAGTTTGTA GAGGACTACT CGGAGCCACA GCCATCTATG 
TTCTACCAGA CCCCTCAGAA CGAGCACATC TACCAGCAGA AGAACAAGCA CCTCATGGAG 
GTCTACGGCT TCAACGACTC CTTTATCAGC TTAGACCCTT CTCAAGATCT GGCCCCCCCT 
CCTGCTCTCC CACCGAAGCA GCGACAGCTG CAGGCCTCCT ATGCTGCCTC TTCTTTCCCT 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGCCCTTCA CCCCCGAGGA CACCGGTGCC 
GCTCAGGGCC TCACTATGTC AGTCTCTAAC TCCTTTCTCA ACCGGCACAG CTCCTTTCCT 
GTGCATTCGG GCTGGAGCAA ACGGCATCTT AAATTGGGAT CCAGTGGAAA ATCCCAGCAA 
AGCAGTAAGC CCTCAGAGTG CTTTTCCCTG CCGAGATGCT TCCAGCACGC TCAGAGCCGT 
GGTTTCTGTA TGCCTTCGCA TCCCTTCAGC CAGCCTGGTG CTGTCCAGGT GTGCTCTGTT 
GCTCACAGCT GCAGCACCAG GCGTCCCTCC GTGCTGACCG CCCCGCTGCC ACCATCCTGC 
CCTGAGCGAG GGCTGCTGTT TGTGGAAGGC CTCACCTCAC CACCAAACCT GCCCGCCCAG 
TACAAGTCTG TGTTTAGGTC CTACTCGCAG GACTTTGTGC CTCACAACCA GGTCTCCGTA 
CCTCCTTTCC TGTCTTCTAC CTCCTCCTCC TGCTCCACCG CGCCCTTTCC GCCCGTCCAC 
CCGTCTCCGA GCTCTGACCT CGCAGTGCCA GCCACGGCCA GCCCGTCTCC CAGCACTGGG 
GATGTGCCAA ACTCCTCTTC GCAGGAGAGC AGCTTTAACG GGAGTGCTCC TGTCTGCCTT 
CCTTCTGAAA CCTCTCTCAC TGATTCTCTG CAGACGCCTT CGAGTGAAAA CGCCAGTGAG 
GAGGCTGGTG AGGGTGAATA CGTCAATCTG TATTCCTCTG GCCAGAGCAA CGGGGAACTC 
CCTCATTCTG GAGGAGAATC TCCCTCTGTC AAAGACGGCC ATTCCAAAGA CTCCACTTCA 
AACAGCAGTG CCATGGGGAA GGAAGGCAAA GATGGTGCTG AGAGGCAGCA GCAGTCGCCA 
GATGCTTTGG AATCGTCACA GTTGGAGGAA GAAGTGGATG AGCTCTCCCT TATTGACCAC 
AGTGAAATTA TGGCTAGGTT AACACTGAAG CAAGAGGGAG ATGATGGGCC AGATGTCCGC 
GGTGGATCTG GAGATATTTT GTTGGTGCAT GCAACAGAGA CCGACAGGAA AGATCTGGTG 
CTGTACTGTG AAGCCTTCCT GACTACATAC AGGACATTTA TTACCCCTGA AGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAGTTT TGCCACTTCC CAGACACGTT TAAGAAGCGA 
GTCAGTAAGA ACACCTTCTT CGTGCTGGTG AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG 
CTGACGGAGG AGATTCTCAA GCTGCTGATG GACCTGGTGT TCCGGCTGGT CTGCAATGGG 
GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA AACATCCTGG ACAAAGTGGA TCAGAAGAAG 
ATGTTGAGAT ACGCCAACTC CATCAAACCT CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG 
GGCACCTTGC ATGATTTCCA CAGTCATGAA ATAGCCGAGC AGCTGACACT CTTAGATGCT 
GAACTCTTCT ATAAAATCGA GATACCAGAA GTTTTGCTTT GGGCGAAAGA GCAAAATGAA 
GAGAAGAGCC CCAACCTGAC GCAGTTCACA GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGTTCCATAA TAATGCTTCA AGAGAAAGCC CAAGACCGAG AGAGGCTGCT GCTTAAGTTC 
ATAAAGATTA TGAAGCACTT ACGAAAGCTG AATAATTTTA ATTCCTATCT GGCCATTCTT 
TCTGCACTGG ATTCTGCACC CATCCGAAGG CTGGAGTGGC AGAAACAAAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACACT GATCGACAGC TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC 
CTGGCTGATG TGGAGCCTCC CTGCATCCCA TACCTAGGCC TAATTCTGCA GGACCTGACC 
TTTGTTCATC TGGGAAACCC AGACTACATC GACAGCAAGG TGAACTTCTC CAAGCGCTGG 
CAGCAGTTTA ACATCCTGGA CAGCATGAGG TGCTTCCAAC AGGTACATTA CGACATCAAG 
AGAAACGACG ACATCGTGTC GTTCTTCAAC GACTTCAGCG ACCATCTGGC GGAGGAGGCC 
CTGTGGGAAC TGTCCCTCAA AATCAAACCT CGGAACATTA CAAGGCGGAA AACAGACCGG 
GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279744.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135230.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

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CloneID	OGa43666
Clone ID Related Accession (Same CDS sequence)	XM_015279744.1 , XM_015279744.2
Accession Version	XM_015279744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGGGCAAGTT TCATTCACCA AAAATAAAGA GAACTCCTTC CAAGAAGGGA 
AAGCAAGCTG ACCTGACAGT GAAAACACCA GAGAAGTCAG CGAACAAAAA CTTATCATGG 
CTAGAGGAGA AGGAGAAGGA GGTCGTCAGT GCTCTGCGCT ACTTCAAGAC GATTGTGGAC 
AAAATGGCAA TTGATAAGAA AGTATTAGAG ATGCTGCCAG GCTCAGCAAG TAAAGTGCTG 
GAAGCCATCC TGCCCCTGGT GCAAAGTGAC CCACGCATCC AACAAAGTTC TGCCATCTCG 
TCCTGCTACA GCCGTGTGTA CCAGAGCCTG GCCAATCTCA TTCGCTGGTC TGATCAAGTG 
ATGCTGGAAG GTGTGAATTC TGAAGACAAA GAGATGGTGA CAACTGTTAA AGACGTCATA 
AAAGCTGTTC TGGATGGAGT TAAGGAGCTG GTCAAACTCA CGATTGAAAA GCAGGAGCAT 
CCCTCTCCCA CAAGCCCTGT TAAACCCAGT TTACCAGCAT GTAAATCTGA TAGCCCATCA 
GAACTTCCCC TTACAGACCG AGAAATGGAG ATCCTAAACA AAACAACTAA TATGACTCAA 
TCAAACGAGC TACTGACAGA CTCCATGGAC GAAGAGGTTG CACCTCCTAA ACCCCCCCTG 
CCTTGCATTC GTGTGGCAGA AAACAGCCCT CCTCCAGCCT TGCCCCCTAA AAAACGGCAG 
TCGGCGCCTT CCCCAACCAG AGTGGCAGTT GTGGCCCCCA TGAGTCGAGC CACCAGTGGG 
TCTAGTTTGC CTGTTGGAAT CAACAGACAG GATTTTGATG TTGACTGCTA TGCCCAGAGA 
AGGCTGTCAG GTGGCAGCCA CTCCTACGGG GGAGAATCTC CTCGCCTCTC ACCGTGCAGC 
AGCATTGGCA AACTCAGCAA GTCCGATGAG CAGCTCTCTT CTCTGGACCG TGACAGTGGT 
CAGTGCTCCC GCAACACAAG CTGTGAAACA TTAGATCAAT CAGATCACTA TGACCCGGAC 
TATGAATTCC TGCAGCAGGA CCTCTCAAAT GCTGATCAGA TACCTCAACA GGTGCCAACT 
ACCCTCAGCC CCTTGCCAGA GTCCCTGGGT GAGTCTGGCT CCCCTTTCCA CGGACATGCT 
TTCCAGCTCC CACAGGGCAG CTCTTCTCCA CTGGAATTCT GCAGCCTCTC GGGAGCTGTG 
CAGCCAACAG AGACCCCTCC AGCTTTACCA GAAAAGAAGA GGAGAAGCGC AGCCTCTCAA 
ACTTCAGACT CAAGCTGCAG GGTCTCATAC GAGAGACACC CTTCACAATA TGATAACATC 
TCAGAGGACG ATATGCAGAA CGCAGGGTCT CTCTCATCAG TACCCTGCAC GCCATTTGCT 
CCTATTTTGC CCTTCCAACA AGGAAACTCT TCAGCACAAG TTGAATTCAT TGCTGATTTT 
GCTGCTCCAG ATTCTAACAG TGACCCAGAG AAGCCACCAC CTCTGCCAGA GAAGAAAAAC 
AAACACATGA TGGCGTACAT GCAGTTTGTA GAGGACTACT CGGAGCCACA GCCATCTATG 
TTCTACCAGA CCCCTCAGAA CGAGCACATC TACCAGCAGA AGAACAAGCA CCTCATGGAG 
GTCTACGGCT TCAACGACTC CTTTATCAGC TTAGACCCTT CTCAAGATCT GGCCCCCCCT 
CCTGCTCTCC CACCGAAGCA GCGACAGCTG CAGGCCTCCT ATGCTGCCTC TTCTTTCCCT 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGCCCTTCA CCCCCGAGGA CACCGGTGCC 
GCTCAGGGCC TCACTATGTC AGTCTCTAAC TCCTTTCTCA ACCGGCACAG CTCCTTTCCT 
GTGCATTCGG GCTGGAGCAA ACGGCATCTT AAATTGGGAT CCAGTGGAAA ATCCCAGCAA 
AGCAGTAAGC CCTCAGAGTG CTTTTCCCTG CCGAGATGCT TCCAGCACGC TCAGAGCCGT 
GGTTTCTGTA TGCCTTCGCA TCCCTTCAGC CAGCCTGGTG CTGTCCAGGT GTGCTCTGTT 
GCTCACAGCT GCAGCACCAG GCGTCCCTCC GTGCTGACCG CCCCGCTGCC ACCATCCTGC 
CCTGAGCGAG GGCTGCTGTT TGTGGAAGGC CTCACCTCAC CACCAAACCT GCCCGCCCAG 
TACAAGTCTG TGTTTAGGTC CTACTCGCAG GACTTTGTGC CTCACAACCA GGTCTCCGTA 
CCTCCTTTCC TGTCTTCTAC CTCCTCCTCC TGCTCCACCG CGCCCTTTCC GCCCGTCCAC 
CCGTCTCCGA GCTCTGACCT CGCAGTGCCA GCCACGGCCA GCCCGTCTCC CAGCACTGGG 
GATGTGCCAA ACTCCTCTTC GCAGGAGAGC AGCTTTAACG GGAGTGCTCC TGTCTGCCTT 
CCTTCTGAAA CCTCTCTCAC TGATTCTCTG CAGACGCCTT CGAGTGAAAA CGCCAGTGAG 
GAGGCTGGTG AGGGTGAATA CGTCAATCTG TATTCCTCTG GCCAGAGCAA CGGGGAACTC 
CCTCATTCTG GAGGAGAATC TCCCTCTGTC AAAGACGGCC ATTCCAAAGA CTCCACTTCA 
AACAGCAGTG CCATGGGGAA GGAAGGCAAA GATGGTGCTG AGAGGCAGCA GCAGTCGCCA 
GATGCTTTGG AATCGTCACA GTTGGAGGAA GAAGTGGATG AGCTCTCCCT TATTGACCAC 
AGTGAAATTA TGGCTAGGTT AACACTGAAG CAAGAGGGAG ATGATGGGCC AGATGTCCGC 
GGTGGATCTG GAGATATTTT GTTGGTGCAT GCAACAGAGA CCGACAGGAA AGATCTGGTG 
CTGTACTGTG AAGCCTTCCT GACTACATAC AGGACATTTA TTACCCCTGA AGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAGTTT TGCCACTTCC CAGACACGTT TAAGAAGCGA 
GTCAGTAAGA ACACCTTCTT CGTGCTGGTG AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG 
CTGACGGAGG AGATTCTCAA GCTGCTGATG GACCTGGTGT TCCGGCTGGT CTGCAATGGG 
GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA AACATCCTGG ACAAAGTGGA TCAGAAGAAG 
ATGTTGAGAT ACGCCAACTC CATCAAACCT CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG 
GGCACCTTGC ATGATTTCCA CAGTCATGAA ATAGCCGAGC AGCTGACACT CTTAGATGCT 
GAACTCTTCT ATAAAATCGA GATACCAGAA GTTTTGCTTT GGGCGAAAGA GCAAAATGAA 
GAGAAGAGCC CCAACCTGAC GCAGTTCACA GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGTTCCATAA TAATGCTTCA AGAGAAAGCC CAAGACCGAG AGAGGCTGCT GCTTAAGTTC 
ATAAAGATTA TGAAGCACTT ACGAAAGCTG AATAATTTTA ATTCCTATCT GGCCATTCTT 
TCTGCACTGG ATTCTGCACC CATCCGAAGG CTGGAGTGGC AGAAACAAAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACACT GATCGACAGC TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC 
CTGGCTGATG TGGAGCCTCC CTGCATCCCA TACCTAGGCC TAATTCTGCA GGACCTGACC 
TTTGTTCATC TGGGAAACCC AGACTACATC GACAGCAAGG TGAACTTCTC CAAGCGCTGG 
CAGCAGTTTA ACATCCTGGA CAGCATGAGG TGCTTCCAAC AGGTACATTA CGACATCAAG 
AGAAACGACG ACATCGTGTC GTTCTTCAAC GACTTCAGCG ACCATCTGGC GGAGGAGGCC 
CTGTGGGAAC TGTCCCTCAA AATCAAACCT CGGAACATTA CAAGGCGGAA AACAGACCGG 
GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135230.1
CDS	129..4043
Translation	MKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279744.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279744.2

ORF Insert Sequence:

ATGAAGCTGA AGGGCAAGTT TCATTCACCA AAAATAAAGA GAACTCCTTC CAAGAAGGGA 
AAGCAAGCTG ACCTGACAGT GAAAACACCA GAGAAGTCAG CGAACAAAAA CTTATCATGG 
CTAGAGGAGA AGGAGAAGGA GGTCGTCAGT GCTCTGCGCT ACTTCAAGAC GATTGTGGAC 
AAAATGGCAA TTGATAAGAA AGTATTAGAG ATGCTGCCAG GCTCAGCAAG TAAAGTGCTG 
GAAGCCATCC TGCCCCTGGT GCAAAGTGAC CCACGCATCC AACAAAGTTC TGCCATCTCG 
TCCTGCTACA GCCGTGTGTA CCAGAGCCTG GCCAATCTCA TTCGCTGGTC TGATCAAGTG 
ATGCTGGAAG GTGTGAATTC TGAAGACAAA GAGATGGTGA CAACTGTTAA AGACGTCATA 
AAAGCTGTTC TGGATGGAGT TAAGGAGCTG GTCAAACTCA CGATTGAAAA GCAGGAGCAT 
CCCTCTCCCA CAAGCCCTGT TAAACCCAGT TTACCAGCAT GTAAATCTGA TAGCCCATCA 
GAACTTCCCC TTACAGACCG AGAAATGGAG ATCCTAAACA AAACAACTAA TATGACTCAA 
TCAAACGAGC TACTGACAGA CTCCATGGAC GAAGAGGTTG CACCTCCTAA ACCCCCCCTG 
CCTTGCATTC GTGTGGCAGA AAACAGCCCT CCTCCAGCCT TGCCCCCTAA AAAACGGCAG 
TCGGCGCCTT CCCCAACCAG AGTGGCAGTT GTGGCCCCCA TGAGTCGAGC CACCAGTGGG 
TCTAGTTTGC CTGTTGGAAT CAACAGACAG GATTTTGATG TTGACTGCTA TGCCCAGAGA 
AGGCTGTCAG GTGGCAGCCA CTCCTACGGG GGAGAATCTC CTCGCCTCTC ACCGTGCAGC 
AGCATTGGCA AACTCAGCAA GTCCGATGAG CAGCTCTCTT CTCTGGACCG TGACAGTGGT 
CAGTGCTCCC GCAACACAAG CTGTGAAACA TTAGATCAAT CAGATCACTA TGACCCGGAC 
TATGAATTCC TGCAGCAGGA CCTCTCAAAT GCTGATCAGA TACCTCAACA GGTGCCAACT 
ACCCTCAGCC CCTTGCCAGA GTCCCTGGGT GAGTCTGGCT CCCCTTTCCA CGGACATGCT 
TTCCAGCTCC CACAGGGCAG CTCTTCTCCA CTGGAATTCT GCAGCCTCTC GGGAGCTGTG 
CAGCCAACAG AGACCCCTCC AGCTTTACCA GAAAAGAAGA GGAGAAGCGC AGCCTCTCAA 
ACTTCAGACT CAAGCTGCAG GGTCTCATAC GAGAGACACC CTTCACAATA TGATAACATC 
TCAGAGGACG ATATGCAGAA CGCAGGGTCT CTCTCATCAG TACCCTGCAC GCCATTTGCT 
CCTATTTTGC CCTTCCAACA AGGAAACTCT TCAGCACAAG TTGAATTCAT TGCTGATTTT 
GCTGCTCCAG ATTCTAACAG TGACCCAGAG AAGCCACCAC CTCTGCCAGA GAAGAAAAAC 
AAACACATGA TGGCGTACAT GCAGTTTGTA GAGGACTACT CGGAGCCACA GCCATCTATG 
TTCTACCAGA CCCCTCAGAA CGAGCACATC TACCAGCAGA AGAACAAGCA CCTCATGGAG 
GTCTACGGCT TCAACGACTC CTTTATCAGC TTAGACCCTT CTCAAGATCT GGCCCCCCCT 
CCTGCTCTCC CACCGAAGCA GCGACAGCTG CAGGCCTCCT ATGCTGCCTC TTCTTTCCCT 
TCTGTCTCCT ACTGTGTCCA GCAAACTAAA GTGCCCTTCA CCCCCGAGGA CACCGGTGCC 
GCTCAGGGCC TCACTATGTC AGTCTCTAAC TCCTTTCTCA ACCGGCACAG CTCCTTTCCT 
GTGCATTCGG GCTGGAGCAA ACGGCATCTT AAATTGGGAT CCAGTGGAAA ATCCCAGCAA 
AGCAGTAAGC CCTCAGAGTG CTTTTCCCTG CCGAGATGCT TCCAGCACGC TCAGAGCCGT 
GGTTTCTGTA TGCCTTCGCA TCCCTTCAGC CAGCCTGGTG CTGTCCAGGT GTGCTCTGTT 
GCTCACAGCT GCAGCACCAG GCGTCCCTCC GTGCTGACCG CCCCGCTGCC ACCATCCTGC 
CCTGAGCGAG GGCTGCTGTT TGTGGAAGGC CTCACCTCAC CACCAAACCT GCCCGCCCAG 
TACAAGTCTG TGTTTAGGTC CTACTCGCAG GACTTTGTGC CTCACAACCA GGTCTCCGTA 
CCTCCTTTCC TGTCTTCTAC CTCCTCCTCC TGCTCCACCG CGCCCTTTCC GCCCGTCCAC 
CCGTCTCCGA GCTCTGACCT CGCAGTGCCA GCCACGGCCA GCCCGTCTCC CAGCACTGGG 
GATGTGCCAA ACTCCTCTTC GCAGGAGAGC AGCTTTAACG GGAGTGCTCC TGTCTGCCTT 
CCTTCTGAAA CCTCTCTCAC TGATTCTCTG CAGACGCCTT CGAGTGAAAA CGCCAGTGAG 
GAGGCTGGTG AGGGTGAATA CGTCAATCTG TATTCCTCTG GCCAGAGCAA CGGGGAACTC 
CCTCATTCTG GAGGAGAATC TCCCTCTGTC AAAGACGGCC ATTCCAAAGA CTCCACTTCA 
AACAGCAGTG CCATGGGGAA GGAAGGCAAA GATGGTGCTG AGAGGCAGCA GCAGTCGCCA 
GATGCTTTGG AATCGTCACA GTTGGAGGAA GAAGTGGATG AGCTCTCCCT TATTGACCAC 
AGTGAAATTA TGGCTAGGTT AACACTGAAG CAAGAGGGAG ATGATGGGCC AGATGTCCGC 
GGTGGATCTG GAGATATTTT GTTGGTGCAT GCAACAGAGA CCGACAGGAA AGATCTGGTG 
CTGTACTGTG AAGCCTTCCT GACTACATAC AGGACATTTA TTACCCCTGA AGAGCTCATC 
AAGAAGCTGC AGTACAGATA TGAGAAGTTT TGCCACTTCC CAGACACGTT TAAGAAGCGA 
GTCAGTAAGA ACACCTTCTT CGTGCTGGTG AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG 
CTGACGGAGG AGATTCTCAA GCTGCTGATG GACCTGGTGT TCCGGCTGGT CTGCAATGGG 
GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA AACATCCTGG ACAAAGTGGA TCAGAAGAAG 
ATGTTGAGAT ACGCCAACTC CATCAAACCT CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG 
GGCACCTTGC ATGATTTCCA CAGTCATGAA ATAGCCGAGC AGCTGACACT CTTAGATGCT 
GAACTCTTCT ATAAAATCGA GATACCAGAA GTTTTGCTTT GGGCGAAAGA GCAAAATGAA 
GAGAAGAGCC CCAACCTGAC GCAGTTCACA GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGTTCCATAA TAATGCTTCA AGAGAAAGCC CAAGACCGAG AGAGGCTGCT GCTTAAGTTC 
ATAAAGATTA TGAAGCACTT ACGAAAGCTG AATAATTTTA ATTCCTATCT GGCCATTCTT 
TCTGCACTGG ATTCTGCACC CATCCGAAGG CTGGAGTGGC AGAAACAAAC CTCAGAGGGC 
CTGGCTGAGT ACTGCACACT GATCGACAGC TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC 
CTGGCTGATG TGGAGCCTCC CTGCATCCCA TACCTAGGCC TAATTCTGCA GGACCTGACC 
TTTGTTCATC TGGGAAACCC AGACTACATC GACAGCAAGG TGAACTTCTC CAAGCGCTGG 
CAGCAGTTTA ACATCCTGGA CAGCATGAGG TGCTTCCAAC AGGTACATTA CGACATCAAG 
AGAAACGACG ACATCGTGTC GTTCTTCAAC GACTTCAGCG ACCATCTGGC GGAGGAGGCC 
CTGTGGGAAC TGTCCCTCAA AATCAAACCT CGGAACATTA CAAGGCGGAA AACAGACCGG 
GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279745.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135231.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa43667
Clone ID Related Accession (Same CDS sequence)	XM_015279745.1 , XM_015279745.2
Accession Version	XM_015279745.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135231.1
CDS	229..4107
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279745.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279745.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279745.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135231.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

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CloneID	OGa43667
Clone ID Related Accession (Same CDS sequence)	XM_015279745.1 , XM_015279745.2
Accession Version	XM_015279745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135231.1
CDS	245..4123
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLQASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSGWSKRHLKLGSSGKSQQSSKPSECFSLPRCFQHAQSRGFCMPSHPFSQPGAVQVCSVAHSCSTRRPSVLTAPLPPSCPERGLLFVEGLTSPPNLPAQYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279745.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279745.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGCAG GCCTCCTATG CTGCCTCTTC TTTCCCTTCT 
GTCTCCTACT GTGTCCAGCA AACTAAAGTG CCCTTCACCC CCGAGGACAC CGGTGCCGCT 
CAGGGCCTCA CTATGTCAGT CTCTAACTCC TTTCTCAACC GGCACAGCTC CTTTCCTGTG 
CATTCGGGCT GGAGCAAACG GCATCTTAAA TTGGGATCCA GTGGAAAATC CCAGCAAAGC 
AGTAAGCCCT CAGAGTGCTT TTCCCTGCCG AGATGCTTCC AGCACGCTCA GAGCCGTGGT 
TTCTGTATGC CTTCGCATCC CTTCAGCCAG CCTGGTGCTG TCCAGGTGTG CTCTGTTGCT 
CACAGCTGCA GCACCAGGCG TCCCTCCGTG CTGACCGCCC CGCTGCCACC ATCCTGCCCT 
GAGCGAGGGC TGCTGTTTGT GGAAGGCCTC ACCTCACCAC CAAACCTGCC CGCCCAGTAC 
AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC TTTGTGCCTC ACAACCAGGT CTCCGTACCT 
CCTTTCCTGT CTTCTACCTC CTCCTCCTGC TCCACCGCGC CCTTTCCGCC CGTCCACCCG 
TCTCCGAGCT CTGACCTCGC AGTGCCAGCC ACGGCCAGCC CGTCTCCCAG CACTGGGGAT 
GTGCCAAACT CCTCTTCGCA GGAGAGCAGC TTTAACGGGA GTGCTCCTGT CTGCCTTCCT 
TCTGAAACCT CTCTCACTGA TTCTCTGCAG ACGCCTTCGG AATCTCCCTC TGTCAAAGAC 
GGCCATTCCA AAGACTCCAC TTCAAACAGC AGTGCCATGG GGAAGGAAGG CAAAGATGGT 
GCTGAGAGGC AGCAGCAGTC GCCAGATGCT TTGGAATCGT CACAGTTGGA GGAAGAAGTG 
GATGAGCTCT CCCTTATTGA CCACAGTGAA ATTATGGCTA GGTTAACACT GAAGCAAGAG 
GGAGATGATG GGCCAGATGT CCGCGGTGGA TCTGGAGATA TTTTGTTGGT GCATGCAACA 
GAGACCGACA GGAAAGATCT GGTGCTGTAC TGTGAAGCCT TCCTGACTAC ATACAGGACA 
TTTATTACCC CTGAAGAGCT CATCAAGAAG CTGCAGTACA GATATGAGAA GTTTTGCCAC 
TTCCCAGACA CGTTTAAGAA GCGAGTCAGT AAGAACACCT TCTTCGTGCT GGTGAGAGTG 
GTGGATGAGC TGTGCTTAGT GGAGCTGACG GAGGAGATTC TCAAGCTGCT GATGGACCTG 
GTGTTCCGGC TGGTCTGCAA TGGGGAGCTG AGCCTCGCCA GAGTGCTGCG CAAAAACATC 
CTGGACAAAG TGGATCAGAA GAAGATGTTG AGATACGCCA ACTCCATCAA ACCTCTCGCA 
GCCCGCGGGG TGGCAGCCAG GCCGGGCACC TTGCATGATT TCCACAGTCA TGAAATAGCC 
GAGCAGCTGA CACTCTTAGA TGCTGAACTC TTCTATAAAA TCGAGATACC AGAAGTTTTG 
CTTTGGGCGA AAGAGCAAAA TGAAGAGAAG AGCCCCAACC TGACGCAGTT CACAGAGCAC 
TTTAATAACA TGTCCTACTG GGTCCGTTCC ATAATAATGC TTCAAGAGAA AGCCCAAGAC 
CGAGAGAGGC TGCTGCTTAA GTTCATAAAG ATTATGAAGC ACTTACGAAA GCTGAATAAT 
TTTAATTCCT ATCTGGCCAT TCTTTCTGCA CTGGATTCTG CACCCATCCG AAGGCTGGAG 
TGGCAGAAAC AAACCTCAGA GGGCCTGGCT GAGTACTGCA CACTGATCGA CAGCTCCTCG 
TCCTTCCGCG CCTACCGTGC AGCCCTGGCT GATGTGGAGC CTCCCTGCAT CCCATACCTA 
GGCCTAATTC TGCAGGACCT GACCTTTGTT CATCTGGGAA ACCCAGACTA CATCGACAGC 
AAGGTGAACT TCTCCAAGCG CTGGCAGCAG TTTAACATCC TGGACAGCAT GAGGTGCTTC 
CAACAGGTAC ATTACGACAT CAAGAGAAAC GACGACATCG TGTCGTTCTT CAACGACTTC 
AGCGACCATC TGGCGGAGGA GGCCCTGTGG GAACTGTCCC TCAAAATCAA ACCTCGGAAC 
ATTACAAGGC GGAAAACAGA CCGGGAAGAG AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279748.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135234.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.

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CloneID	OGa43668
Clone ID Related Accession (Same CDS sequence)	XM_015279748.1 , XM_015279748.2
Accession Version	XM_015279748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGTACAAGT CTGTGTTTAG GTCCTACTCG CAGGACTTTG TGCCTCACAA CCAGGTCTCC 
GTACCTCCTT TCCTGTCTTC TACCTCCTCC TCCTGCTCCA CCGCGCCCTT TCCGCCCGTC 
CACCCGTCTC CGAGCTCTGA CCTCGCAGTG CCAGCCACGG CCAGCCCGTC TCCCAGCACT 
GGGGATGTGC CAAACTCCTC TTCGCAGGAG AGCAGCTTTA ACGGGAGTGC TCCTGTCTGC 
CTTCCTTCTG AAACCTCTCT CACTGATTCT CTGCAGACGC CTTCGGAATC TCCCTCTGTC 
AAAGACGGCC ATTCCAAAGA CTCCACTTCA AACAGCAGTG CCATGGGGAA GGAAGGCAAA 
GATGGTGCTG AGAGGCAGCA GCAGTCGCCA GATGCTTTGG AATCGTCACA GTTGGAGGAA 
GAAGTGGATG AGCTCTCCCT TATTGACCAC AGTGAAATTA TGGCTAGGTT AACACTGAAG 
CAAGAGGGAG ATGATGGGCC AGATGTCCGC GGTGGATCTG GAGATATTTT GTTGGTGCAT 
GCAACAGAGA CCGACAGGAA AGATCTGGTG CTGTACTGTG AAGCCTTCCT GACTACATAC 
AGGACATTTA TTACCCCTGA AGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAGTTT 
TGCCACTTCC CAGACACGTT TAAGAAGCGA GTCAGTAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG CTGACGGAGG AGATTCTCAA GCTGCTGATG 
GACCTGGTGT TCCGGCTGGT CTGCAATGGG GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA 
AACATCCTGG ACAAAGTGGA TCAGAAGAAG ATGTTGAGAT ACGCCAACTC CATCAAACCT 
CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG GGCACCTTGC ATGATTTCCA CAGTCATGAA 
ATAGCCGAGC AGCTGACACT CTTAGATGCT GAACTCTTCT ATAAAATCGA GATACCAGAA 
GTTTTGCTTT GGGCGAAAGA GCAAAATGAA GAGAAGAGCC CCAACCTGAC GCAGTTCACA 
GAGCACTTTA ATAACATGTC CTACTGGGTC CGTTCCATAA TAATGCTTCA AGAGAAAGCC 
CAAGACCGAG AGAGGCTGCT GCTTAAGTTC ATAAAGATTA TGAAGCACTT ACGAAAGCTG 
AATAATTTTA ATTCCTATCT GGCCATTCTT TCTGCACTGG ATTCTGCACC CATCCGAAGG 
CTGGAGTGGC AGAAACAAAC CTCAGAGGGC CTGGCTGAGT ACTGCACACT GATCGACAGC 
TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC CTGGCTGATG TGGAGCCTCC CTGCATCCCA 
TACCTAGGCC TAATTCTGCA GGACCTGACC TTTGTTCATC TGGGAAACCC AGACTACATC 
GACAGCAAGG TGAACTTCTC CAAGCGCTGG CAGCAGTTTA ACATCCTGGA CAGCATGAGG 
TGCTTCCAAC AGGTACATTA CGACATCAAG AGAAACGACG ACATCGTGTC GTTCTTCAAC 
GACTTCAGCG ACCATCTGGC GGAGGAGGCC CTGTGGGAAC TGTCCCTCAA AATCAAACCT 
CGGAACATTA CAAGGCGGAA AACAGACCGG GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135234.1
CDS	228..3812
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279748.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279748.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGTACAAGT CTGTGTTTAG GTCCTACTCG CAGGACTTTG TGCCTCACAA CCAGGTCTCC 
GTACCTCCTT TCCTGTCTTC TACCTCCTCC TCCTGCTCCA CCGCGCCCTT TCCGCCCGTC 
CACCCGTCTC CGAGCTCTGA CCTCGCAGTG CCAGCCACGG CCAGCCCGTC TCCCAGCACT 
GGGGATGTGC CAAACTCCTC TTCGCAGGAG AGCAGCTTTA ACGGGAGTGC TCCTGTCTGC 
CTTCCTTCTG AAACCTCTCT CACTGATTCT CTGCAGACGC CTTCGGAATC TCCCTCTGTC 
AAAGACGGCC ATTCCAAAGA CTCCACTTCA AACAGCAGTG CCATGGGGAA GGAAGGCAAA 
GATGGTGCTG AGAGGCAGCA GCAGTCGCCA GATGCTTTGG AATCGTCACA GTTGGAGGAA 
GAAGTGGATG AGCTCTCCCT TATTGACCAC AGTGAAATTA TGGCTAGGTT AACACTGAAG 
CAAGAGGGAG ATGATGGGCC AGATGTCCGC GGTGGATCTG GAGATATTTT GTTGGTGCAT 
GCAACAGAGA CCGACAGGAA AGATCTGGTG CTGTACTGTG AAGCCTTCCT GACTACATAC 
AGGACATTTA TTACCCCTGA AGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAGTTT 
TGCCACTTCC CAGACACGTT TAAGAAGCGA GTCAGTAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG CTGACGGAGG AGATTCTCAA GCTGCTGATG 
GACCTGGTGT TCCGGCTGGT CTGCAATGGG GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA 
AACATCCTGG ACAAAGTGGA TCAGAAGAAG ATGTTGAGAT ACGCCAACTC CATCAAACCT 
CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG GGCACCTTGC ATGATTTCCA CAGTCATGAA 
ATAGCCGAGC AGCTGACACT CTTAGATGCT GAACTCTTCT ATAAAATCGA GATACCAGAA 
GTTTTGCTTT GGGCGAAAGA GCAAAATGAA GAGAAGAGCC CCAACCTGAC GCAGTTCACA 
GAGCACTTTA ATAACATGTC CTACTGGGTC CGTTCCATAA TAATGCTTCA AGAGAAAGCC 
CAAGACCGAG AGAGGCTGCT GCTTAAGTTC ATAAAGATTA TGAAGCACTT ACGAAAGCTG 
AATAATTTTA ATTCCTATCT GGCCATTCTT TCTGCACTGG ATTCTGCACC CATCCGAAGG 
CTGGAGTGGC AGAAACAAAC CTCAGAGGGC CTGGCTGAGT ACTGCACACT GATCGACAGC 
TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC CTGGCTGATG TGGAGCCTCC CTGCATCCCA 
TACCTAGGCC TAATTCTGCA GGACCTGACC TTTGTTCATC TGGGAAACCC AGACTACATC 
GACAGCAAGG TGAACTTCTC CAAGCGCTGG CAGCAGTTTA ACATCCTGGA CAGCATGAGG 
TGCTTCCAAC AGGTACATTA CGACATCAAG AGAAACGACG ACATCGTGTC GTTCTTCAAC 
GACTTCAGCG ACCATCTGGC GGAGGAGGCC CTGTGGGAAC TGTCCCTCAA AATCAAACCT 
CGGAACATTA CAAGGCGGAA AACAGACCGG GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279748.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135234.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

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CloneID	OGa43668
Clone ID Related Accession (Same CDS sequence)	XM_015279748.1 , XM_015279748.2
Accession Version	XM_015279748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGTACAAGT CTGTGTTTAG GTCCTACTCG CAGGACTTTG TGCCTCACAA CCAGGTCTCC 
GTACCTCCTT TCCTGTCTTC TACCTCCTCC TCCTGCTCCA CCGCGCCCTT TCCGCCCGTC 
CACCCGTCTC CGAGCTCTGA CCTCGCAGTG CCAGCCACGG CCAGCCCGTC TCCCAGCACT 
GGGGATGTGC CAAACTCCTC TTCGCAGGAG AGCAGCTTTA ACGGGAGTGC TCCTGTCTGC 
CTTCCTTCTG AAACCTCTCT CACTGATTCT CTGCAGACGC CTTCGGAATC TCCCTCTGTC 
AAAGACGGCC ATTCCAAAGA CTCCACTTCA AACAGCAGTG CCATGGGGAA GGAAGGCAAA 
GATGGTGCTG AGAGGCAGCA GCAGTCGCCA GATGCTTTGG AATCGTCACA GTTGGAGGAA 
GAAGTGGATG AGCTCTCCCT TATTGACCAC AGTGAAATTA TGGCTAGGTT AACACTGAAG 
CAAGAGGGAG ATGATGGGCC AGATGTCCGC GGTGGATCTG GAGATATTTT GTTGGTGCAT 
GCAACAGAGA CCGACAGGAA AGATCTGGTG CTGTACTGTG AAGCCTTCCT GACTACATAC 
AGGACATTTA TTACCCCTGA AGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAGTTT 
TGCCACTTCC CAGACACGTT TAAGAAGCGA GTCAGTAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG CTGACGGAGG AGATTCTCAA GCTGCTGATG 
GACCTGGTGT TCCGGCTGGT CTGCAATGGG GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA 
AACATCCTGG ACAAAGTGGA TCAGAAGAAG ATGTTGAGAT ACGCCAACTC CATCAAACCT 
CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG GGCACCTTGC ATGATTTCCA CAGTCATGAA 
ATAGCCGAGC AGCTGACACT CTTAGATGCT GAACTCTTCT ATAAAATCGA GATACCAGAA 
GTTTTGCTTT GGGCGAAAGA GCAAAATGAA GAGAAGAGCC CCAACCTGAC GCAGTTCACA 
GAGCACTTTA ATAACATGTC CTACTGGGTC CGTTCCATAA TAATGCTTCA AGAGAAAGCC 
CAAGACCGAG AGAGGCTGCT GCTTAAGTTC ATAAAGATTA TGAAGCACTT ACGAAAGCTG 
AATAATTTTA ATTCCTATCT GGCCATTCTT TCTGCACTGG ATTCTGCACC CATCCGAAGG 
CTGGAGTGGC AGAAACAAAC CTCAGAGGGC CTGGCTGAGT ACTGCACACT GATCGACAGC 
TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC CTGGCTGATG TGGAGCCTCC CTGCATCCCA 
TACCTAGGCC TAATTCTGCA GGACCTGACC TTTGTTCATC TGGGAAACCC AGACTACATC 
GACAGCAAGG TGAACTTCTC CAAGCGCTGG CAGCAGTTTA ACATCCTGGA CAGCATGAGG 
TGCTTCCAAC AGGTACATTA CGACATCAAG AGAAACGACG ACATCGTGTC GTTCTTCAAC 
GACTTCAGCG ACCATCTGGC GGAGGAGGCC CTGTGGGAAC TGTCCCTCAA AATCAAACCT 
CGGAACATTA CAAGGCGGAA AACAGACCGG GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135234.1
CDS	242..3826
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLASYAASSFPSVSYCVQQTKVPFTPEDTGAAQGLTMSVSNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279748.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279748.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGGCC TCCTATGCTG CCTCTTCTTT CCCTTCTGTC 
TCCTACTGTG TCCAGCAAAC TAAAGTGCCC TTCACCCCCG AGGACACCGG TGCCGCTCAG 
GGCCTCACTA TGTCAGTCTC TAACTCCTTT CTCAACCGGC ACAGCTCCTT TCCTGTGCAT 
TCGTACAAGT CTGTGTTTAG GTCCTACTCG CAGGACTTTG TGCCTCACAA CCAGGTCTCC 
GTACCTCCTT TCCTGTCTTC TACCTCCTCC TCCTGCTCCA CCGCGCCCTT TCCGCCCGTC 
CACCCGTCTC CGAGCTCTGA CCTCGCAGTG CCAGCCACGG CCAGCCCGTC TCCCAGCACT 
GGGGATGTGC CAAACTCCTC TTCGCAGGAG AGCAGCTTTA ACGGGAGTGC TCCTGTCTGC 
CTTCCTTCTG AAACCTCTCT CACTGATTCT CTGCAGACGC CTTCGGAATC TCCCTCTGTC 
AAAGACGGCC ATTCCAAAGA CTCCACTTCA AACAGCAGTG CCATGGGGAA GGAAGGCAAA 
GATGGTGCTG AGAGGCAGCA GCAGTCGCCA GATGCTTTGG AATCGTCACA GTTGGAGGAA 
GAAGTGGATG AGCTCTCCCT TATTGACCAC AGTGAAATTA TGGCTAGGTT AACACTGAAG 
CAAGAGGGAG ATGATGGGCC AGATGTCCGC GGTGGATCTG GAGATATTTT GTTGGTGCAT 
GCAACAGAGA CCGACAGGAA AGATCTGGTG CTGTACTGTG AAGCCTTCCT GACTACATAC 
AGGACATTTA TTACCCCTGA AGAGCTCATC AAGAAGCTGC AGTACAGATA TGAGAAGTTT 
TGCCACTTCC CAGACACGTT TAAGAAGCGA GTCAGTAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ATGAGCTGTG CTTAGTGGAG CTGACGGAGG AGATTCTCAA GCTGCTGATG 
GACCTGGTGT TCCGGCTGGT CTGCAATGGG GAGCTGAGCC TCGCCAGAGT GCTGCGCAAA 
AACATCCTGG ACAAAGTGGA TCAGAAGAAG ATGTTGAGAT ACGCCAACTC CATCAAACCT 
CTCGCAGCCC GCGGGGTGGC AGCCAGGCCG GGCACCTTGC ATGATTTCCA CAGTCATGAA 
ATAGCCGAGC AGCTGACACT CTTAGATGCT GAACTCTTCT ATAAAATCGA GATACCAGAA 
GTTTTGCTTT GGGCGAAAGA GCAAAATGAA GAGAAGAGCC CCAACCTGAC GCAGTTCACA 
GAGCACTTTA ATAACATGTC CTACTGGGTC CGTTCCATAA TAATGCTTCA AGAGAAAGCC 
CAAGACCGAG AGAGGCTGCT GCTTAAGTTC ATAAAGATTA TGAAGCACTT ACGAAAGCTG 
AATAATTTTA ATTCCTATCT GGCCATTCTT TCTGCACTGG ATTCTGCACC CATCCGAAGG 
CTGGAGTGGC AGAAACAAAC CTCAGAGGGC CTGGCTGAGT ACTGCACACT GATCGACAGC 
TCCTCGTCCT TCCGCGCCTA CCGTGCAGCC CTGGCTGATG TGGAGCCTCC CTGCATCCCA 
TACCTAGGCC TAATTCTGCA GGACCTGACC TTTGTTCATC TGGGAAACCC AGACTACATC 
GACAGCAAGG TGAACTTCTC CAAGCGCTGG CAGCAGTTTA ACATCCTGGA CAGCATGAGG 
TGCTTCCAAC AGGTACATTA CGACATCAAG AGAAACGACG ACATCGTGTC GTTCTTCAAC 
GACTTCAGCG ACCATCTGGC GGAGGAGGCC CTGTGGGAAC TGTCCCTCAA AATCAAACCT 
CGGAACATTA CAAGGCGGAA AACAGACCGG GAAGAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279749.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135235.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.

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CloneID	OGa43669
Clone ID Related Accession (Same CDS sequence)	XM_015279749.1 , XM_015279749.2
Accession Version	XM_015279749.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGTAC AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC 
TTTGTGCCTC ACAACCAGGT CTCCGTACCT CCTTTCCTGT CTTCTACCTC CTCCTCCTGC 
TCCACCGCGC CCTTTCCGCC CGTCCACCCG TCTCCGAGCT CTGACCTCGC AGTGCCAGCC 
ACGGCCAGCC CGTCTCCCAG CACTGGGGAT GTGCCAAACT CCTCTTCGCA GGAGAGCAGC 
TTTAACGGGA GTGCTCCTGT CTGCCTTCCT TCTGAAACCT CTCTCACTGA TTCTCTGCAG 
ACGCCTTCGA GTGAAAACGC CAGTGAGGAG GCTGGTGAGG GTGAATACGT CAATCTGTAT 
TCCTCTGGCC AGAGCAACGG GGAACTCCCT CATTCTGGAG GAGAATCTCC CTCTGTCAAA 
GACGGCCATT CCAAAGACTC CACTTCAAAC AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT 
GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT GCTTTGGAAT CGTCACAGTT GGAGGAAGAA 
GTGGATGAGC TCTCCCTTAT TGACCACAGT GAAATTATGG CTAGGTTAAC ACTGAAGCAA 
GAGGGAGATG ATGGGCCAGA TGTCCGCGGT GGATCTGGAG ATATTTTGTT GGTGCATGCA 
ACAGAGACCG ACAGGAAAGA TCTGGTGCTG TACTGTGAAG CCTTCCTGAC TACATACAGG 
ACATTTATTA CCCCTGAAGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAGTTTTGC 
CACTTCCCAG ACACGTTTAA GAAGCGAGTC AGTAAGAACA CCTTCTTCGT GCTGGTGAGA 
GTGGTGGATG AGCTGTGCTT AGTGGAGCTG ACGGAGGAGA TTCTCAAGCT GCTGATGGAC 
CTGGTGTTCC GGCTGGTCTG CAATGGGGAG CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC 
ATCCTGGACA AAGTGGATCA GAAGAAGATG TTGAGATACG CCAACTCCAT CAAACCTCTC 
GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC ACCTTGCATG ATTTCCACAG TCATGAAATA 
GCCGAGCAGC TGACACTCTT AGATGCTGAA CTCTTCTATA AAATCGAGAT ACCAGAAGTT 
TTGCTTTGGG CGAAAGAGCA AAATGAAGAG AAGAGCCCCA ACCTGACGCA GTTCACAGAG 
CACTTTAATA ACATGTCCTA CTGGGTCCGT TCCATAATAA TGCTTCAAGA GAAAGCCCAA 
GACCGAGAGA GGCTGCTGCT TAAGTTCATA AAGATTATGA AGCACTTACG AAAGCTGAAT 
AATTTTAATT CCTATCTGGC CATTCTTTCT GCACTGGATT CTGCACCCAT CCGAAGGCTG 
GAGTGGCAGA AACAAACCTC AGAGGGCCTG GCTGAGTACT GCACACTGAT CGACAGCTCC 
TCGTCCTTCC GCGCCTACCG TGCAGCCCTG GCTGATGTGG AGCCTCCCTG CATCCCATAC 
CTAGGCCTAA TTCTGCAGGA CCTGACCTTT GTTCATCTGG GAAACCCAGA CTACATCGAC 
AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG CAGTTTAACA TCCTGGACAG CATGAGGTGC 
TTCCAACAGG TACATTACGA CATCAAGAGA AACGACGACA TCGTGTCGTT CTTCAACGAC 
TTCAGCGACC ATCTGGCGGA GGAGGCCCTG TGGGAACTGT CCCTCAAAAT CAAACCTCGG 
AACATTACAA GGCGGAAAAC AGACCGGGAA GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135235.1
CDS	227..3748
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279749.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279749.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGTAC AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC 
TTTGTGCCTC ACAACCAGGT CTCCGTACCT CCTTTCCTGT CTTCTACCTC CTCCTCCTGC 
TCCACCGCGC CCTTTCCGCC CGTCCACCCG TCTCCGAGCT CTGACCTCGC AGTGCCAGCC 
ACGGCCAGCC CGTCTCCCAG CACTGGGGAT GTGCCAAACT CCTCTTCGCA GGAGAGCAGC 
TTTAACGGGA GTGCTCCTGT CTGCCTTCCT TCTGAAACCT CTCTCACTGA TTCTCTGCAG 
ACGCCTTCGA GTGAAAACGC CAGTGAGGAG GCTGGTGAGG GTGAATACGT CAATCTGTAT 
TCCTCTGGCC AGAGCAACGG GGAACTCCCT CATTCTGGAG GAGAATCTCC CTCTGTCAAA 
GACGGCCATT CCAAAGACTC CACTTCAAAC AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT 
GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT GCTTTGGAAT CGTCACAGTT GGAGGAAGAA 
GTGGATGAGC TCTCCCTTAT TGACCACAGT GAAATTATGG CTAGGTTAAC ACTGAAGCAA 
GAGGGAGATG ATGGGCCAGA TGTCCGCGGT GGATCTGGAG ATATTTTGTT GGTGCATGCA 
ACAGAGACCG ACAGGAAAGA TCTGGTGCTG TACTGTGAAG CCTTCCTGAC TACATACAGG 
ACATTTATTA CCCCTGAAGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAGTTTTGC 
CACTTCCCAG ACACGTTTAA GAAGCGAGTC AGTAAGAACA CCTTCTTCGT GCTGGTGAGA 
GTGGTGGATG AGCTGTGCTT AGTGGAGCTG ACGGAGGAGA TTCTCAAGCT GCTGATGGAC 
CTGGTGTTCC GGCTGGTCTG CAATGGGGAG CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC 
ATCCTGGACA AAGTGGATCA GAAGAAGATG TTGAGATACG CCAACTCCAT CAAACCTCTC 
GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC ACCTTGCATG ATTTCCACAG TCATGAAATA 
GCCGAGCAGC TGACACTCTT AGATGCTGAA CTCTTCTATA AAATCGAGAT ACCAGAAGTT 
TTGCTTTGGG CGAAAGAGCA AAATGAAGAG AAGAGCCCCA ACCTGACGCA GTTCACAGAG 
CACTTTAATA ACATGTCCTA CTGGGTCCGT TCCATAATAA TGCTTCAAGA GAAAGCCCAA 
GACCGAGAGA GGCTGCTGCT TAAGTTCATA AAGATTATGA AGCACTTACG AAAGCTGAAT 
AATTTTAATT CCTATCTGGC CATTCTTTCT GCACTGGATT CTGCACCCAT CCGAAGGCTG 
GAGTGGCAGA AACAAACCTC AGAGGGCCTG GCTGAGTACT GCACACTGAT CGACAGCTCC 
TCGTCCTTCC GCGCCTACCG TGCAGCCCTG GCTGATGTGG AGCCTCCCTG CATCCCATAC 
CTAGGCCTAA TTCTGCAGGA CCTGACCTTT GTTCATCTGG GAAACCCAGA CTACATCGAC 
AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG CAGTTTAACA TCCTGGACAG CATGAGGTGC 
TTCCAACAGG TACATTACGA CATCAAGAGA AACGACGACA TCGTGTCGTT CTTCAACGAC 
TTCAGCGACC ATCTGGCGGA GGAGGCCCTG TGGGAACTGT CCCTCAAAAT CAAACCTCGG 
AACATTACAA GGCGGAAAAC AGACCGGGAA GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_015279749.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135235.1 Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa43669
Clone ID Related Accession (Same CDS sequence)	XM_015279749.1 , XM_015279749.2
Accession Version	XM_015279749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGTAC AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC 
TTTGTGCCTC ACAACCAGGT CTCCGTACCT CCTTTCCTGT CTTCTACCTC CTCCTCCTGC 
TCCACCGCGC CCTTTCCGCC CGTCCACCCG TCTCCGAGCT CTGACCTCGC AGTGCCAGCC 
ACGGCCAGCC CGTCTCCCAG CACTGGGGAT GTGCCAAACT CCTCTTCGCA GGAGAGCAGC 
TTTAACGGGA GTGCTCCTGT CTGCCTTCCT TCTGAAACCT CTCTCACTGA TTCTCTGCAG 
ACGCCTTCGA GTGAAAACGC CAGTGAGGAG GCTGGTGAGG GTGAATACGT CAATCTGTAT 
TCCTCTGGCC AGAGCAACGG GGAACTCCCT CATTCTGGAG GAGAATCTCC CTCTGTCAAA 
GACGGCCATT CCAAAGACTC CACTTCAAAC AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT 
GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT GCTTTGGAAT CGTCACAGTT GGAGGAAGAA 
GTGGATGAGC TCTCCCTTAT TGACCACAGT GAAATTATGG CTAGGTTAAC ACTGAAGCAA 
GAGGGAGATG ATGGGCCAGA TGTCCGCGGT GGATCTGGAG ATATTTTGTT GGTGCATGCA 
ACAGAGACCG ACAGGAAAGA TCTGGTGCTG TACTGTGAAG CCTTCCTGAC TACATACAGG 
ACATTTATTA CCCCTGAAGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAGTTTTGC 
CACTTCCCAG ACACGTTTAA GAAGCGAGTC AGTAAGAACA CCTTCTTCGT GCTGGTGAGA 
GTGGTGGATG AGCTGTGCTT AGTGGAGCTG ACGGAGGAGA TTCTCAAGCT GCTGATGGAC 
CTGGTGTTCC GGCTGGTCTG CAATGGGGAG CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC 
ATCCTGGACA AAGTGGATCA GAAGAAGATG TTGAGATACG CCAACTCCAT CAAACCTCTC 
GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC ACCTTGCATG ATTTCCACAG TCATGAAATA 
GCCGAGCAGC TGACACTCTT AGATGCTGAA CTCTTCTATA AAATCGAGAT ACCAGAAGTT 
TTGCTTTGGG CGAAAGAGCA AAATGAAGAG AAGAGCCCCA ACCTGACGCA GTTCACAGAG 
CACTTTAATA ACATGTCCTA CTGGGTCCGT TCCATAATAA TGCTTCAAGA GAAAGCCCAA 
GACCGAGAGA GGCTGCTGCT TAAGTTCATA AAGATTATGA AGCACTTACG AAAGCTGAAT 
AATTTTAATT CCTATCTGGC CATTCTTTCT GCACTGGATT CTGCACCCAT CCGAAGGCTG 
GAGTGGCAGA AACAAACCTC AGAGGGCCTG GCTGAGTACT GCACACTGAT CGACAGCTCC 
TCGTCCTTCC GCGCCTACCG TGCAGCCCTG GCTGATGTGG AGCCTCCCTG CATCCCATAC 
CTAGGCCTAA TTCTGCAGGA CCTGACCTTT GTTCATCTGG GAAACCCAGA CTACATCGAC 
AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG CAGTTTAACA TCCTGGACAG CATGAGGTGC 
TTCCAACAGG TACATTACGA CATCAAGAGA AACGACGACA TCGTGTCGTT CTTCAACGAC 
TTCAGCGACC ATCTGGCGGA GGAGGCCCTG TGGGAACTGT CCCTCAAAAT CAAACCTCGG 
AACATTACAA GGCGGAAAAC AGACCGGGAA GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135235.1
CDS	242..3763
Translation	MSGKIEKADSERSHLSSFTMKLKGKFHSPKIKRTPSKKGKQADLTVKTPEKSANKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPSLPACKSDSPSELPLTDREMEILNKTTNMTQSNELLTDSMDEEVAPPKPPLPCIRVAENSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDQSDHYDPDYEFLQQDLSNADQIPQQVPTTLSPLPESLGESGSPFHGHAFQLPQGSSSPLEFCSLSGAVQPTETPPALPEKKRRSAASQTSDSSCRVSYERHPSQYDNISEDDMQNAGSLSSVPCTPFAPILPFQQGNSSAQVEFIADFAAPDSNSDPEKPPPLPEKKNKHMMAYMQFVEDYSEPQPSMFYQTPQNEHIYQQKNKHLMEVYGFNDSFISLDPSQDLAPPPALPPKQRQLYKSVFRSYSQDFVPHNQVSVPPFLSSTSSSCSTAPFPPVHPSPSSDLAVPATASPSPSTGDVPNSSSQESSFNGSAPVCLPSETSLTDSLQTPSSENASEEAGEGEYVNLYSSGQSNGELPHSGGESPSVKDGHSKDSTSNSSAMGKEGKDGAERQQQSPDALESSQLEEEVDELSLIDHSEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCHFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMDLVFRLVCNGELSLARVLRKNILDKVDQKKMLRYANSIKPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDYIDSKVNFSKRWQQFNILDSMRCFQQVHYDIKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_015279749.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279749.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC GAGCGCTCCC ATCTCTCCTC GTTCACTATG 
AAGCTGAAGG GCAAGTTTCA TTCACCAAAA ATAAAGAGAA CTCCTTCCAA GAAGGGAAAG 
CAAGCTGACC TGACAGTGAA AACACCAGAG AAGTCAGCGA ACAAAAACTT ATCATGGCTA 
GAGGAGAAGG AGAAGGAGGT CGTCAGTGCT CTGCGCTACT TCAAGACGAT TGTGGACAAA 
ATGGCAATTG ATAAGAAAGT ATTAGAGATG CTGCCAGGCT CAGCAAGTAA AGTGCTGGAA 
GCCATCCTGC CCCTGGTGCA AAGTGACCCA CGCATCCAAC AAAGTTCTGC CATCTCGTCC 
TGCTACAGCC GTGTGTACCA GAGCCTGGCC AATCTCATTC GCTGGTCTGA TCAAGTGATG 
CTGGAAGGTG TGAATTCTGA AGACAAAGAG ATGGTGACAA CTGTTAAAGA CGTCATAAAA 
GCTGTTCTGG ATGGAGTTAA GGAGCTGGTC AAACTCACGA TTGAAAAGCA GGAGCATCCC 
TCTCCCACAA GCCCTGTTAA ACCCAGTTTA CCAGCATGTA AATCTGATAG CCCATCAGAA 
CTTCCCCTTA CAGACCGAGA AATGGAGATC CTAAACAAAA CAACTAATAT GACTCAATCA 
AACGAGCTAC TGACAGACTC CATGGACGAA GAGGTTGCAC CTCCTAAACC CCCCCTGCCT 
TGCATTCGTG TGGCAGAAAA CAGCCCTCCT CCAGCCTTGC CCCCTAAAAA ACGGCAGTCG 
GCGCCTTCCC CAACCAGAGT GGCAGTTGTG GCCCCCATGA GTCGAGCCAC CAGTGGGTCT 
AGTTTGCCTG TTGGAATCAA CAGACAGGAT TTTGATGTTG ACTGCTATGC CCAGAGAAGG 
CTGTCAGGTG GCAGCCACTC CTACGGGGGA GAATCTCCTC GCCTCTCACC GTGCAGCAGC 
ATTGGCAAAC TCAGCAAGTC CGATGAGCAG CTCTCTTCTC TGGACCGTGA CAGTGGTCAG 
TGCTCCCGCA ACACAAGCTG TGAAACATTA GATCAATCAG ATCACTATGA CCCGGACTAT 
GAATTCCTGC AGCAGGACCT CTCAAATGCT GATCAGATAC CTCAACAGGT GCCAACTACC 
CTCAGCCCCT TGCCAGAGTC CCTGGGTGAG TCTGGCTCCC CTTTCCACGG ACATGCTTTC 
CAGCTCCCAC AGGGCAGCTC TTCTCCACTG GAATTCTGCA GCCTCTCGGG AGCTGTGCAG 
CCAACAGAGA CCCCTCCAGC TTTACCAGAA AAGAAGAGGA GAAGCGCAGC CTCTCAAACT 
TCAGACTCAA GCTGCAGGGT CTCATACGAG AGACACCCTT CACAATATGA TAACATCTCA 
GAGGACGATA TGCAGAACGC AGGGTCTCTC TCATCAGTAC CCTGCACGCC ATTTGCTCCT 
ATTTTGCCCT TCCAACAAGG AAACTCTTCA GCACAAGTTG AATTCATTGC TGATTTTGCT 
GCTCCAGATT CTAACAGTGA CCCAGAGAAG CCACCACCTC TGCCAGAGAA GAAAAACAAA 
CACATGATGG CGTACATGCA GTTTGTAGAG GACTACTCGG AGCCACAGCC ATCTATGTTC 
TACCAGACCC CTCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCACCT CATGGAGGTC 
TACGGCTTCA ACGACTCCTT TATCAGCTTA GACCCTTCTC AAGATCTGGC CCCCCCTCCT 
GCTCTCCCAC CGAAGCAGCG ACAGCTGTAC AAGTCTGTGT TTAGGTCCTA CTCGCAGGAC 
TTTGTGCCTC ACAACCAGGT CTCCGTACCT CCTTTCCTGT CTTCTACCTC CTCCTCCTGC 
TCCACCGCGC CCTTTCCGCC CGTCCACCCG TCTCCGAGCT CTGACCTCGC AGTGCCAGCC 
ACGGCCAGCC CGTCTCCCAG CACTGGGGAT GTGCCAAACT CCTCTTCGCA GGAGAGCAGC 
TTTAACGGGA GTGCTCCTGT CTGCCTTCCT TCTGAAACCT CTCTCACTGA TTCTCTGCAG 
ACGCCTTCGA GTGAAAACGC CAGTGAGGAG GCTGGTGAGG GTGAATACGT CAATCTGTAT 
TCCTCTGGCC AGAGCAACGG GGAACTCCCT CATTCTGGAG GAGAATCTCC CTCTGTCAAA 
GACGGCCATT CCAAAGACTC CACTTCAAAC AGCAGTGCCA TGGGGAAGGA AGGCAAAGAT 
GGTGCTGAGA GGCAGCAGCA GTCGCCAGAT GCTTTGGAAT CGTCACAGTT GGAGGAAGAA 
GTGGATGAGC TCTCCCTTAT TGACCACAGT GAAATTATGG CTAGGTTAAC ACTGAAGCAA 
GAGGGAGATG ATGGGCCAGA TGTCCGCGGT GGATCTGGAG ATATTTTGTT GGTGCATGCA 
ACAGAGACCG ACAGGAAAGA TCTGGTGCTG TACTGTGAAG CCTTCCTGAC TACATACAGG 
ACATTTATTA CCCCTGAAGA GCTCATCAAG AAGCTGCAGT ACAGATATGA GAAGTTTTGC 
CACTTCCCAG ACACGTTTAA GAAGCGAGTC AGTAAGAACA CCTTCTTCGT GCTGGTGAGA 
GTGGTGGATG AGCTGTGCTT AGTGGAGCTG ACGGAGGAGA TTCTCAAGCT GCTGATGGAC 
CTGGTGTTCC GGCTGGTCTG CAATGGGGAG CTGAGCCTCG CCAGAGTGCT GCGCAAAAAC 
ATCCTGGACA AAGTGGATCA GAAGAAGATG TTGAGATACG CCAACTCCAT CAAACCTCTC 
GCAGCCCGCG GGGTGGCAGC CAGGCCGGGC ACCTTGCATG ATTTCCACAG TCATGAAATA 
GCCGAGCAGC TGACACTCTT AGATGCTGAA CTCTTCTATA AAATCGAGAT ACCAGAAGTT 
TTGCTTTGGG CGAAAGAGCA AAATGAAGAG AAGAGCCCCA ACCTGACGCA GTTCACAGAG 
CACTTTAATA ACATGTCCTA CTGGGTCCGT TCCATAATAA TGCTTCAAGA GAAAGCCCAA 
GACCGAGAGA GGCTGCTGCT TAAGTTCATA AAGATTATGA AGCACTTACG AAAGCTGAAT 
AATTTTAATT CCTATCTGGC CATTCTTTCT GCACTGGATT CTGCACCCAT CCGAAGGCTG 
GAGTGGCAGA AACAAACCTC AGAGGGCCTG GCTGAGTACT GCACACTGAT CGACAGCTCC 
TCGTCCTTCC GCGCCTACCG TGCAGCCCTG GCTGATGTGG AGCCTCCCTG CATCCCATAC 
CTAGGCCTAA TTCTGCAGGA CCTGACCTTT GTTCATCTGG GAAACCCAGA CTACATCGAC 
AGCAAGGTGA ACTTCTCCAA GCGCTGGCAG CAGTTTAACA TCCTGGACAG CATGAGGTGC 
TTCCAACAGG TACATTACGA CATCAAGAGA AACGACGACA TCGTGTCGTT CTTCAACGAC 
TTCAGCGACC ATCTGGCGGA GGAGGCCCTG TGGGAACTGT CCCTCAAAAT CAAACCTCGG 
AACATTACAA GGCGGAAAAC AGACCGGGAA GAGAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF1
Entrez Gene ID	417173
Full Name	Rap guanine nucleotide exchange factor 1
General protein information	Preferred Names Rap guanine nucleotide exchange factor 1 Names rap guanine nucleotide exchange factor 1 Rap guanine nucleotide exchange factor (GEF) 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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