AgaP_AGAP001527 cDNA ORF clone, Anopheles gambiae str. PEST

Gene
Clones
gRNAs

Summary

Gene Symbol	AgaP_AGAP001527
Entrez Gene ID	1281652
Full Name	AGAP001527-PA
Synonyms	AgaP_ENSANGG00000007199,ENSANGG00000007199
Gene Type	protein-coding
Organism	Anopheles gambiae str. PEST

mRNA and Protein(s)

mRNA	Protein	Name
XM_321598.5	XP_321598.5	AGAP001527-PA

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Gene Ontology
Bibliography

Related articles in PubMed

The Anopheles gambiae genome: an update.
Mongin E, Louis C, Holt RA, Birney E, Collins FH
Trends in parasitology20(2)49-52(2004 Feb)

The genome sequence of the malaria mosquito Anopheles gambiae.
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
Science (New York, N.Y.)298(5591)129-49(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AgaP_AGAP001527 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP001527 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAh07508	XM_321598.5 Latest version!	Anopheles gambiae str. PEST AGAP001527-RA (AgaP_AGAP001527), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AgaP_AGAP001527 ( XM_321598.5 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_321598.5 Anopheles gambiae str. PEST AGAP001527-RA (AgaP_AGAP001527), partial mRNA.

Price & Availability↑

CloneID	OAh07508
Clone ID Related Accession (Same CDS sequence)	XM_321598.5
Accession Version	XM_321598.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP001527-PA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078266). On Sep 28, 2011 this sequence version replaced XM_321598.4. COMPLETENESS: incomplete on the 5' end.

ATGTCACCGC CCGGGAATGG AATATTCGTC GTGCGGGGTG AAATGTCCAC CCTGACGACG 
GCAATGCGCC GCGGGTCCAG GTGGAGCTTC AACACGTATC AGGACGACGA TAAGGATGTG 
CTGTTGAAGA GTTTCCAGGA GCTGAAAGAG GTGCTGCTGC AGGTGGAGGA TCTTCGGCTG 
GTGGAACCGA ACGTGTTTCT TTCGCCGTTT CTGAACGTGA TCCGGTCGGA GGAAACGACC 
GGCCCCGTCA CCAGCCTCGC CCTGTCGGCC GTGAACAAGT TCCTGTCGTA CGGGCTGATC 
GATCCAACAC ACTCGACCCT GGCGGCCACG GTGGAAAACA TTGCCGATGC GGTGACGCAC 
GCACGGTTCG TCGGCACGGA CCAAACGTCC GACGGGGTGG TGCTGATGAA GATCATACAG 
GTGCTCCGTA CGCTGGTGCT CAGCCCGGAA GGCAGCGCCC TGTCGGACGA AAGCATGTGC 
GACATCATCC TGAGTTGCTT TCGGCTGTGC TTTGAGCCGC GCCTCAACGA GCTGGTGCGT 
CGCACGGCAG AGAATGCGCT GAAAGACATC GTGCTGCTGC TGTTCATGCG GCTGCCCCAG 
TTCGTCGAGG GTGGAAGCTA CAACACGCTC AAGACGCTAA AGATGCGCTC CAGCTCGATG 
GATCAGTCGG GCAAGCGAAG AAAATACAGC AAGACCAGCA TGAAGGAGGA ACAGAAACCA 
GCCCCCACTG CCGTGCCGAG CACGAACGCG GCTGCTCCGG TGGTTCCGAA GATCGTGACG 
GAAGACCCCA CGAACGTGGC CGGATCGGGA GAGGAACCGG TCACGCCAGT AATGCTGACG 
GCACCGAACC GTCTCAAGCC GATGCCACTG TCCACTACAC CGGCCACTCC AGCGGGTGTT 
ATCGTCGATC TGCAGGGAAC GATATCGCAA ACACCTAAAG CTCCTCTCCC ATCGGAATCG 
GCCGAACGGC AGTCGATTGC ATCAGCGGAA GATGGAATTT CCAACGCAGA GACCGAGCTG 
GAAACCGTTA CAGCGCAGGG CACAGAGGAG GGTACCACCG CCGCCGACCC TTCGTTTGTG 
AACTCCGTTG GCGTGCGATT CACGTCCCAG CAGGCGGAAG AAGACGTTCC GCAGGTGGCG 
AATGGGGCCG CCACCGTTAC ACATCTCCCG TACGGATTGC CCTGCATACG GGAGCTGTTT 
CGGTTTCTGA TTTCGCTGTG CAATCCCATG GACAAGCAGA ACGGTGACGT CATGATCCAC 
ATGGGCCTGA CGCTGCTGAC CGTGACGTTC GAGGTGGGCG CCGACAGCAT CGGTCGGTAC 
GATTCGCTGA TCGCGATCGT GAAGGACGAC CTGTGCCGCA ATCTGTTCGC GCTGCTCGCC 
ACGGAGCGCA TTTCGATCTT TGCGGCCGGG TTGCAGCTGT CCTTTCTGCT GTTCGAGTCG 
CTCCGATCGC AGCTCAAGTT CCAGCTCGAG CACTACCTCA CCCGCGTGGC GGACATGATC 
ATGAACGACA GCCCCCGGAT ACTGTACGAG GCGCGCGAAC TGGCGATGGA CAATCTGCTG 
CAGCTGTGGC GCATTCCGGG CTTTGCGGCC GAGCTGTACA TCAACTACGA CTGCGATCTG 
TACTGTTCGA ACCTGTTCGA GGACCTGACG AAGCTGCTGT CGAAAAACAC GCTTTCCGCA 
ACGCAAGCCA TCTACAGCAT CCACACACTG TCGATGGACG CGCTGCTGAC GATCGTCGAA 
TCGATCGAGC GCAACTGCGC GCAGGCGAAG AACGGCCAGA AGCCGAAGTA TATGCGCCAT 
TCGCGGAACA ACTCCTACGC AACGGCAAAG ATCGTGCTGG ACGCCGGCAC GCCACAAGCC 
GCCCGTGAAG CCGGTGGCCA GGTAGCGCTC GAGCTGGAGG AAGGCGACTC GGAAGAAGGG 
GCCGGCAATA CCGAACCGGC CGTGCTGGTG GAGAATATTA GCAAATTTCT ACACTCTAGT 
CAAAGGGATC GCATTAGCCG GGTAGCGGCC GGGATCGGTA CGGATGCATC CGGCGGGGAA 
GATCCCAGCA CCGCCGCCGA GGGTGCCTCG CTCTCCGAGG CCGTGTCGCA GAAGCTAACG 
CACGAAGAGC TGGCGGCAAT AAAGCGCAAG AAGCGCTTGC TAACGCAGGG AACGGATCTG 
TTTAACCAGC GGCCGGAAAA GGGCATACAG TTTCTGCAGG AGAACGGGCT GCTGAATCCG 
GTGCTCGACC CGCAGGAGGT GGCACAGTTT CTGCGCGAAA ACTCGGGCCT CGACAAGAAG 
ATGATTGGCG AGTACATTAG CAAGAAGAAG AACGTGGAAA GCCGCATCCT GGAGGTGTAC 
GTGAAGTCGT TCGATTTTGC CGGCCTGACC ATCGACCAGG CGCTGCGGTT GTATCTGGAA 
ACGTTCCGTC TGCCCGGCGA AGCGCCGTTG ATATCGCTTG TGATGGAACA TTTTGCCGAC 
CACTGGCATG AATGCAACAA CGAACCGTTC GCCAATACGG ACGCTGCCTT CCGGCTGGCG 
TACGCAGTGA TCATGCTCAA CATGGACCAG CATAATCACA ATGCGAAGCG GCTGAACGTG 
CCGATGACGG TGGAGGATTT CCTGCGCAAT CTGCGCGGAT TGAACGGCAA CTCGGACTTT 
GATCAGGAAA TGCTAACGAA GATTTATCAC GCAATAAGAA ACGAAGAAAT CGTAATGCCC 
GCGGAACAGA CGGGCCAGGT GCGTGAGAAC TATCTGTGGA AGGTGCTGCT GCGCCGCGGT 
GCCACGAAGG ACGGCATTTT CCATCACGTG TTTGGGCCCC AGCATGATCG CGAGCTGTAT 
CGCGTCATCC AGGGCTCGAC GTTGGCCGCC CTTAGCTTTG TGTTTGACAA ATCGCTCGAC 
AACGCATCGC TCTACCAGAA AGCGATCGGC GGATTCATGA AGTCGGCCGC CATTGCGGCC 
CACTTTCAGC TGCACGGCGA TTTCGATGCG CTCGTGCTGA CGCTGTGCAA GTTCACGACG 
CTGCTGACGC CGCCCCCGAA CGATGCGCAC GAAATTACGG CGAGCGTAAT GTTCGGCCAG 
AACGTGAAGG CGCAGCTGGC GATGCGCACC GTGTTCGGGC TGATCCACGA GCATGGCGAT 
TGTATGCGCG AGGGCTGGCG GCACACGATG GATGTGCTGC TGCAGCTGTT CAAGCTGAAG 
CTGCTGCCGA AGGCGCTGAT GGAGGCGGAA GACTTCTGCG AGGCAAGCGG CAAGGTGACC 
CTGCTGCGCG AGCCCAACCC ACTGCCGAAG ACGGAGGCGG GTCTGTTCAG CTCGCTGTAC 
TCGTACCTGG CGAACGACGG GCAGCGGCAG CCATCGTACG AGGAGCAGGA GGTGATCAAG 
CTGTCGCGCA AGTGTGTCCG TGACTGCCAG ATCGAGCAGA TCGTGAACGA GTCCAAGTTT 
CTACAGCTGG AATCGCTCGA AGAGCTGATC GGGTGCCTGC TGGCGATGAT CGTACCGCCG 
GAGGCGCACA AATCGACCGC CCCGGCGTAC GGCGAGTGTA CGGTCGTGTT TCTGCTCGAG 
CTGCTGGTGA AGGTGCTGAT ACAGAATCGC GATCGCTTGC TGCCAGTTTG GGGCCGGGTG 
CAGGACAAGC TGTACACCCT GCTGGTGGGC GCCTCGGCGC ACGAGTACAC CTACCTGCTG 
CAGCGTACGA CGGTGGCACT GCTCAAGCTG GCCATCTACC TGATGCGCAA CGAGGAAATC 
TGCTCCACCA TCCTGCAGAG CTTGCGGATG CTGCTGGCCC TGCGGCCGGC CGTCATACTG 
GCCATCTCGA AACCGATCTC GATCGGCATG TACGAGCTGC TGAAAACGAG CGCCCAAAAC 
ATCCACACCG AGGCGGACTG GGTGATCGTG TTCACGGTGC TGGAGTGCGT CGGGGCCGGT 
GCCGTGCCGC CGGAGTGCCA CGCGGTCGAG CAGGTGGCAG CAGTGAGCGG TGCCAAGTCG 
GACGGTGCCT TGAGCAGCGA GGAAGACTCC GGCCTGCCGG ACCGGGGCTA CATTTCTGAC 
TCGGAGCTCA GCTCGACGGC CACGATGCCG CTGGTGAAGA CGGCGGCCCC AACTCCACCG 
CCGGCACCGC TTCCCAGCAG CCCGTCCGCG GGCGATGCGT GGATAATGGT GAACAAGGAC 
GCGATCGATC TAGTGTCCGG CGGGTCCGGG GGGCACTCGC CGGGAACGCC CGTTCGGCAC 
TCGATCCAGT ACCACTGCAA GCTGCTCGAG CACTCGCCGT TCGCGCTGGT CAAGTGTTGG 
GAAAGCTTGG CGTTCATCGT GCGCAACGTA GCGCACATCA CGCCGTACAA CTTTGAGAGC 
TGTGTCCGCT GCATCCGCAC CTTTGTCGAG GCTTCGATGG GCCAGACGCA TCAACATCAC 
CAGCAGCAGC GTGACTATCA GGCAGCCAAA AAGAAGCCCG GATCGGGCAA GGATCGTCAG 
CGGAAGCTGC GGGATGGAGC CTCCCACCAG GGGTCCGGGT TGTCCTCTTC GTCGTCCTCC 
TCGCTGGCCA TGGACAGCAG TGACAGTGAG TCGGAAGAGC TGCCCGAGCG TTACCAGTCG 
ATATCGATTC AGCTGCTCGA CCTGATGCAC ACACTGCACA CGCGCACGGC ACAAATCTTC 
CGCTGGTGGG CCGAGGAAGG TGGTGCCGTG CCGCAGTGCT CTTCGCTCTG GTCGCAGGGC 
TGGTGTCCGC TGCTGCAGGG CATCGCACGA CTCGCCACCG ACCAGCGGCG CCAGGTGCGT 
ACCAGTGCGA TCACGTGCCT GCAGCGCGCA CTGCTCGTCC AGGACCTTCA GACGCTGACT 
GGGCTGGAGT GGGCCGGATG TTTCAAGCAG GTGCTGTTCC CACTGCTCCA AGAGCTGTTG 
CAGGAAAAGG CAACCAGTCC GGTGGAGGTG TCGCTGCTTG AAGAATCCCG CATCAGGACG 
GCCACCATCA TGTCGAAGGT GTTTCTGCAC CACTTGACAC CGTTGATCGC TTTGCCCAAC 
TTCCAGGAGC TGTGGATCGA GATACTGGAC TATTTCGAGC GGTTCATGAC GGCTGGCTCC 
GATATGTTGT ACGAGGCAGT GCTGGAGAGC CTGAAAAACA TGCTCCTTGT TATGCATTCG 
GTATGCGTGT TTCACAATAC CGACGGTGTT ACCCATTCTA TGCTGTGGGA CGTTACCTGG 
CAGCGCATAT CCGGATTTCT GCCCAACCTC AAGGATGAGC TGTTCAAACA CGAAGCCGTC 
CGATCGTCGG TTAGTATTAG TGGCGGAACA GGAGGAAATG CGGGTACCGG AGCGGCAAGC 
CCTACCGTAC CACCCTCCCA TACGACGGTT CCAACGGCTC CACCGTCACT TGCAGGAGAG 
CAGCAGCACA TCCTGGAAGG CGGTCAGATA AACAACAGCT CCGCCGTTGG AGAAAGTATT 
AACCCGCGCG TCGTGCTACA TCCGATCCCC ATATCGACCG CAGCGACGGG GTCTGTGCTT 
TCGGCCTCTC CTCCAAACTC TTATATTCAG CAGCTGCAAC AGCCACAACC AATGCCGATC 
CTCCCTGTGA GTCACTCGAT ACCGGCAGTT GAGGTTCATC GTACTCCAGT GCACCGGGCA 
GCACCTTCAA ACAATCTGCT AATGAGCAGT ACCGCCACGA GTGATTTTAA TACCCTTCCG 
ATGGTGTTGC CACGTGTCTC AACGCTACCA TCACCGACTG GAGTAGCAGA ATCATCGATC 
ACGGGGCTTG TGGAGCCTGC CAATCTACTC CTTTCGTCAC CTGCACGAGA TGCTGTAACG 
CAGCCGCAGC CAGCGGTGTC CGCGCCTCCA TCAGTTGCGC CAGCTGCATC GAACATAATT 
GAACTTCCCG GAATCGTAGG CACATCTTGT CCACCACCAC CAGCACCACC GCAACAGTTG 
CCAACGGAGC GACGGGAAGA ACAGGGGCAG AGTGAAACAG GAAGCGAACG CGCTCCAGTC 
GTCACCGATG TTGAGACGGG CAACGTTCCG ATGAGCCATC TTCTGGCCGG TTCGCAGTAC 
CCTTCGCTAC AGCACGTACC GATCGGCATT GCGCAAAGCT TTGCGCCGAT CTTCGTACAA 
CCGAACCCGG CACCAGCGGA AGCGTCTGAC ATCTACTCGG ACTACATTAA TGATCCCTAC 
AATCTGACAC TGCAAATTGA CAACGGAGCG CCGGGTAGCG CCCCCAGTAG TAGCGTTACT 
GCCCCAGAAC CAGCACCACC GCAATCGGCA ACCGCCCAAC CGGACACGTT GTCAAGGACG 
CTAGAGGACG CGAAAACGGG TGGCCCGATG CTGACGGGCT CGACGACAGT GACAGCGACG 
CCGTCTGCCC AGCTGGCGAA CGTGTTTCAG TCGGCGCAAT ACTTTAGCTT CCCGGCCAGT 
GGCCACATAC CGCCCGGCTC GGAGATGCTT TTCGGGGAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_321598.5
CDS	1..6405
Translation	MSPPGNGIFVVRGEMSTLTTAMRRGSRWSFNTYQDDDKDVLLKSFQELKEVLLQVEDLRLVEPNVFLSPFLNVIRSEETTGPVTSLALSAVNKFLSYGLIDPTHSTLAATVENIADAVTHARFVGTDQTSDGVVLMKIIQVLRTLVLSPEGSALSDESMCDIILSCFRLCFEPRLNELVRRTAENALKDIVLLLFMRLPQFVEGGSYNTLKTLKMRSSSMDQSGKRRKYSKTSMKEEQKPAPTAVPSTNAAAPVVPKIVTEDPTNVAGSGEEPVTPVMLTAPNRLKPMPLSTTPATPAGVIVDLQGTISQTPKAPLPSESAERQSIASAEDGISNAETELETVTAQGTEEGTTAADPSFVNSVGVRFTSQQAEEDVPQVANGAATVTHLPYGLPCIRELFRFLISLCNPMDKQNGDVMIHMGLTLLTVTFEVGADSIGRYDSLIAIVKDDLCRNLFALLATERISIFAAGLQLSFLLFESLRSQLKFQLEHYLTRVADMIMNDSPRILYEARELAMDNLLQLWRIPGFAAELYINYDCDLYCSNLFEDLTKLLSKNTLSATQAIYSIHTLSMDALLTIVESIERNCAQAKNGQKPKYMRHSRNNSYATAKIVLDAGTPQAAREAGGQVALELEEGDSEEGAGNTEPAVLVENISKFLHSSQRDRISRVAAGIGTDASGGEDPSTAAEGASLSEAVSQKLTHEELAAIKRKKRLLTQGTDLFNQRPEKGIQFLQENGLLNPVLDPQEVAQFLRENSGLDKKMIGEYISKKKNVESRILEVYVKSFDFAGLTIDQALRLYLETFRLPGEAPLISLVMEHFADHWHECNNEPFANTDAAFRLAYAVIMLNMDQHNHNAKRLNVPMTVEDFLRNLRGLNGNSDFDQEMLTKIYHAIRNEEIVMPAEQTGQVRENYLWKVLLRRGATKDGIFHHVFGPQHDRELYRVIQGSTLAALSFVFDKSLDNASLYQKAIGGFMKSAAIAAHFQLHGDFDALVLTLCKFTTLLTPPPNDAHEITASVMFGQNVKAQLAMRTVFGLIHEHGDCMREGWRHTMDVLLQLFKLKLLPKALMEAEDFCEASGKVTLLREPNPLPKTEAGLFSSLYSYLANDGQRQPSYEEQEVIKLSRKCVRDCQIEQIVNESKFLQLESLEELIGCLLAMIVPPEAHKSTAPAYGECTVVFLLELLVKVLIQNRDRLLPVWGRVQDKLYTLLVGASAHEYTYLLQRTTVALLKLAIYLMRNEEICSTILQSLRMLLALRPAVILAISKPISIGMYELLKTSAQNIHTEADWVIVFTVLECVGAGAVPPECHAVEQVAAVSGAKSDGALSSEEDSGLPDRGYISDSELSSTATMPLVKTAAPTPPPAPLPSSPSAGDAWIMVNKDAIDLVSGGSGGHSPGTPVRHSIQYHCKLLEHSPFALVKCWESLAFIVRNVAHITPYNFESCVRCIRTFVEASMGQTHQHHQQQRDYQAAKKKPGSGKDRQRKLRDGASHQGSGLSSSSSSSLAMDSSDSESEELPERYQSISIQLLDLMHTLHTRTAQIFRWWAEEGGAVPQCSSLWSQGWCPLLQGIARLATDQRRQVRTSAITCLQRALLVQDLQTLTGLEWAGCFKQVLFPLLQELLQEKATSPVEVSLLEESRIRTATIMSKVFLHHLTPLIALPNFQELWIEILDYFERFMTAGSDMLYEAVLESLKNMLLVMHSVCVFHNTDGVTHSMLWDVTWQRISGFLPNLKDELFKHEAVRSSVSISGGTGGNAGTGAASPTVPPSHTTVPTAPPSLAGEQQHILEGGQINNSSAVGESINPRVVLHPIPISTAATGSVLSASPPNSYIQQLQQPQPMPILPVSHSIPAVEVHRTPVHRAAPSNNLLMSSTATSDFNTLPMVLPRVSTLPSPTGVAESSITGLVEPANLLLSSPARDAVTQPQPAVSAPPSVAPAASNIIELPGIVGTSCPPPPAPPQQLPTERREEQGQSETGSERAPVVTDVETGNVPMSHLLAGSQYPSLQHVPIGIAQSFAPIFVQPNPAPAEASDIYSDYINDPYNLTLQIDNGAPGSAPSSSVTAPEPAPPQSATAQPDTLSRTLEDAKTGGPMLTGSTTVTATPSAQLANVFQSAQYFSFPASGHIPPGSEMLFGEP

Target ORF information:

RefSeq Version	XM_321598.5
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP001527-RA (AgaP_AGAP001527), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_321598.5

ORF Insert Sequence:

ATGTCACCGC CCGGGAATGG AATATTCGTC GTGCGGGGTG AAATGTCCAC CCTGACGACG 
GCAATGCGCC GCGGGTCCAG GTGGAGCTTC AACACGTATC AGGACGACGA TAAGGATGTG 
CTGTTGAAGA GTTTCCAGGA GCTGAAAGAG GTGCTGCTGC AGGTGGAGGA TCTTCGGCTG 
GTGGAACCGA ACGTGTTTCT TTCGCCGTTT CTGAACGTGA TCCGGTCGGA GGAAACGACC 
GGCCCCGTCA CCAGCCTCGC CCTGTCGGCC GTGAACAAGT TCCTGTCGTA CGGGCTGATC 
GATCCAACAC ACTCGACCCT GGCGGCCACG GTGGAAAACA TTGCCGATGC GGTGACGCAC 
GCACGGTTCG TCGGCACGGA CCAAACGTCC GACGGGGTGG TGCTGATGAA GATCATACAG 
GTGCTCCGTA CGCTGGTGCT CAGCCCGGAA GGCAGCGCCC TGTCGGACGA AAGCATGTGC 
GACATCATCC TGAGTTGCTT TCGGCTGTGC TTTGAGCCGC GCCTCAACGA GCTGGTGCGT 
CGCACGGCAG AGAATGCGCT GAAAGACATC GTGCTGCTGC TGTTCATGCG GCTGCCCCAG 
TTCGTCGAGG GTGGAAGCTA CAACACGCTC AAGACGCTAA AGATGCGCTC CAGCTCGATG 
GATCAGTCGG GCAAGCGAAG AAAATACAGC AAGACCAGCA TGAAGGAGGA ACAGAAACCA 
GCCCCCACTG CCGTGCCGAG CACGAACGCG GCTGCTCCGG TGGTTCCGAA GATCGTGACG 
GAAGACCCCA CGAACGTGGC CGGATCGGGA GAGGAACCGG TCACGCCAGT AATGCTGACG 
GCACCGAACC GTCTCAAGCC GATGCCACTG TCCACTACAC CGGCCACTCC AGCGGGTGTT 
ATCGTCGATC TGCAGGGAAC GATATCGCAA ACACCTAAAG CTCCTCTCCC ATCGGAATCG 
GCCGAACGGC AGTCGATTGC ATCAGCGGAA GATGGAATTT CCAACGCAGA GACCGAGCTG 
GAAACCGTTA CAGCGCAGGG CACAGAGGAG GGTACCACCG CCGCCGACCC TTCGTTTGTG 
AACTCCGTTG GCGTGCGATT CACGTCCCAG CAGGCGGAAG AAGACGTTCC GCAGGTGGCG 
AATGGGGCCG CCACCGTTAC ACATCTCCCG TACGGATTGC CCTGCATACG GGAGCTGTTT 
CGGTTTCTGA TTTCGCTGTG CAATCCCATG GACAAGCAGA ACGGTGACGT CATGATCCAC 
ATGGGCCTGA CGCTGCTGAC CGTGACGTTC GAGGTGGGCG CCGACAGCAT CGGTCGGTAC 
GATTCGCTGA TCGCGATCGT GAAGGACGAC CTGTGCCGCA ATCTGTTCGC GCTGCTCGCC 
ACGGAGCGCA TTTCGATCTT TGCGGCCGGG TTGCAGCTGT CCTTTCTGCT GTTCGAGTCG 
CTCCGATCGC AGCTCAAGTT CCAGCTCGAG CACTACCTCA CCCGCGTGGC GGACATGATC 
ATGAACGACA GCCCCCGGAT ACTGTACGAG GCGCGCGAAC TGGCGATGGA CAATCTGCTG 
CAGCTGTGGC GCATTCCGGG CTTTGCGGCC GAGCTGTACA TCAACTACGA CTGCGATCTG 
TACTGTTCGA ACCTGTTCGA GGACCTGACG AAGCTGCTGT CGAAAAACAC GCTTTCCGCA 
ACGCAAGCCA TCTACAGCAT CCACACACTG TCGATGGACG CGCTGCTGAC GATCGTCGAA 
TCGATCGAGC GCAACTGCGC GCAGGCGAAG AACGGCCAGA AGCCGAAGTA TATGCGCCAT 
TCGCGGAACA ACTCCTACGC AACGGCAAAG ATCGTGCTGG ACGCCGGCAC GCCACAAGCC 
GCCCGTGAAG CCGGTGGCCA GGTAGCGCTC GAGCTGGAGG AAGGCGACTC GGAAGAAGGG 
GCCGGCAATA CCGAACCGGC CGTGCTGGTG GAGAATATTA GCAAATTTCT ACACTCTAGT 
CAAAGGGATC GCATTAGCCG GGTAGCGGCC GGGATCGGTA CGGATGCATC CGGCGGGGAA 
GATCCCAGCA CCGCCGCCGA GGGTGCCTCG CTCTCCGAGG CCGTGTCGCA GAAGCTAACG 
CACGAAGAGC TGGCGGCAAT AAAGCGCAAG AAGCGCTTGC TAACGCAGGG AACGGATCTG 
TTTAACCAGC GGCCGGAAAA GGGCATACAG TTTCTGCAGG AGAACGGGCT GCTGAATCCG 
GTGCTCGACC CGCAGGAGGT GGCACAGTTT CTGCGCGAAA ACTCGGGCCT CGACAAGAAG 
ATGATTGGCG AGTACATTAG CAAGAAGAAG AACGTGGAAA GCCGCATCCT GGAGGTGTAC 
GTGAAGTCGT TCGATTTTGC CGGCCTGACC ATCGACCAGG CGCTGCGGTT GTATCTGGAA 
ACGTTCCGTC TGCCCGGCGA AGCGCCGTTG ATATCGCTTG TGATGGAACA TTTTGCCGAC 
CACTGGCATG AATGCAACAA CGAACCGTTC GCCAATACGG ACGCTGCCTT CCGGCTGGCG 
TACGCAGTGA TCATGCTCAA CATGGACCAG CATAATCACA ATGCGAAGCG GCTGAACGTG 
CCGATGACGG TGGAGGATTT CCTGCGCAAT CTGCGCGGAT TGAACGGCAA CTCGGACTTT 
GATCAGGAAA TGCTAACGAA GATTTATCAC GCAATAAGAA ACGAAGAAAT CGTAATGCCC 
GCGGAACAGA CGGGCCAGGT GCGTGAGAAC TATCTGTGGA AGGTGCTGCT GCGCCGCGGT 
GCCACGAAGG ACGGCATTTT CCATCACGTG TTTGGGCCCC AGCATGATCG CGAGCTGTAT 
CGCGTCATCC AGGGCTCGAC GTTGGCCGCC CTTAGCTTTG TGTTTGACAA ATCGCTCGAC 
AACGCATCGC TCTACCAGAA AGCGATCGGC GGATTCATGA AGTCGGCCGC CATTGCGGCC 
CACTTTCAGC TGCACGGCGA TTTCGATGCG CTCGTGCTGA CGCTGTGCAA GTTCACGACG 
CTGCTGACGC CGCCCCCGAA CGATGCGCAC GAAATTACGG CGAGCGTAAT GTTCGGCCAG 
AACGTGAAGG CGCAGCTGGC GATGCGCACC GTGTTCGGGC TGATCCACGA GCATGGCGAT 
TGTATGCGCG AGGGCTGGCG GCACACGATG GATGTGCTGC TGCAGCTGTT CAAGCTGAAG 
CTGCTGCCGA AGGCGCTGAT GGAGGCGGAA GACTTCTGCG AGGCAAGCGG CAAGGTGACC 
CTGCTGCGCG AGCCCAACCC ACTGCCGAAG ACGGAGGCGG GTCTGTTCAG CTCGCTGTAC 
TCGTACCTGG CGAACGACGG GCAGCGGCAG CCATCGTACG AGGAGCAGGA GGTGATCAAG 
CTGTCGCGCA AGTGTGTCCG TGACTGCCAG ATCGAGCAGA TCGTGAACGA GTCCAAGTTT 
CTACAGCTGG AATCGCTCGA AGAGCTGATC GGGTGCCTGC TGGCGATGAT CGTACCGCCG 
GAGGCGCACA AATCGACCGC CCCGGCGTAC GGCGAGTGTA CGGTCGTGTT TCTGCTCGAG 
CTGCTGGTGA AGGTGCTGAT ACAGAATCGC GATCGCTTGC TGCCAGTTTG GGGCCGGGTG 
CAGGACAAGC TGTACACCCT GCTGGTGGGC GCCTCGGCGC ACGAGTACAC CTACCTGCTG 
CAGCGTACGA CGGTGGCACT GCTCAAGCTG GCCATCTACC TGATGCGCAA CGAGGAAATC 
TGCTCCACCA TCCTGCAGAG CTTGCGGATG CTGCTGGCCC TGCGGCCGGC CGTCATACTG 
GCCATCTCGA AACCGATCTC GATCGGCATG TACGAGCTGC TGAAAACGAG CGCCCAAAAC 
ATCCACACCG AGGCGGACTG GGTGATCGTG TTCACGGTGC TGGAGTGCGT CGGGGCCGGT 
GCCGTGCCGC CGGAGTGCCA CGCGGTCGAG CAGGTGGCAG CAGTGAGCGG TGCCAAGTCG 
GACGGTGCCT TGAGCAGCGA GGAAGACTCC GGCCTGCCGG ACCGGGGCTA CATTTCTGAC 
TCGGAGCTCA GCTCGACGGC CACGATGCCG CTGGTGAAGA CGGCGGCCCC AACTCCACCG 
CCGGCACCGC TTCCCAGCAG CCCGTCCGCG GGCGATGCGT GGATAATGGT GAACAAGGAC 
GCGATCGATC TAGTGTCCGG CGGGTCCGGG GGGCACTCGC CGGGAACGCC CGTTCGGCAC 
TCGATCCAGT ACCACTGCAA GCTGCTCGAG CACTCGCCGT TCGCGCTGGT CAAGTGTTGG 
GAAAGCTTGG CGTTCATCGT GCGCAACGTA GCGCACATCA CGCCGTACAA CTTTGAGAGC 
TGTGTCCGCT GCATCCGCAC CTTTGTCGAG GCTTCGATGG GCCAGACGCA TCAACATCAC 
CAGCAGCAGC GTGACTATCA GGCAGCCAAA AAGAAGCCCG GATCGGGCAA GGATCGTCAG 
CGGAAGCTGC GGGATGGAGC CTCCCACCAG GGGTCCGGGT TGTCCTCTTC GTCGTCCTCC 
TCGCTGGCCA TGGACAGCAG TGACAGTGAG TCGGAAGAGC TGCCCGAGCG TTACCAGTCG 
ATATCGATTC AGCTGCTCGA CCTGATGCAC ACACTGCACA CGCGCACGGC ACAAATCTTC 
CGCTGGTGGG CCGAGGAAGG TGGTGCCGTG CCGCAGTGCT CTTCGCTCTG GTCGCAGGGC 
TGGTGTCCGC TGCTGCAGGG CATCGCACGA CTCGCCACCG ACCAGCGGCG CCAGGTGCGT 
ACCAGTGCGA TCACGTGCCT GCAGCGCGCA CTGCTCGTCC AGGACCTTCA GACGCTGACT 
GGGCTGGAGT GGGCCGGATG TTTCAAGCAG GTGCTGTTCC CACTGCTCCA AGAGCTGTTG 
CAGGAAAAGG CAACCAGTCC GGTGGAGGTG TCGCTGCTTG AAGAATCCCG CATCAGGACG 
GCCACCATCA TGTCGAAGGT GTTTCTGCAC CACTTGACAC CGTTGATCGC TTTGCCCAAC 
TTCCAGGAGC TGTGGATCGA GATACTGGAC TATTTCGAGC GGTTCATGAC GGCTGGCTCC 
GATATGTTGT ACGAGGCAGT GCTGGAGAGC CTGAAAAACA TGCTCCTTGT TATGCATTCG 
GTATGCGTGT TTCACAATAC CGACGGTGTT ACCCATTCTA TGCTGTGGGA CGTTACCTGG 
CAGCGCATAT CCGGATTTCT GCCCAACCTC AAGGATGAGC TGTTCAAACA CGAAGCCGTC 
CGATCGTCGG TTAGTATTAG TGGCGGAACA GGAGGAAATG CGGGTACCGG AGCGGCAAGC 
CCTACCGTAC CACCCTCCCA TACGACGGTT CCAACGGCTC CACCGTCACT TGCAGGAGAG 
CAGCAGCACA TCCTGGAAGG CGGTCAGATA AACAACAGCT CCGCCGTTGG AGAAAGTATT 
AACCCGCGCG TCGTGCTACA TCCGATCCCC ATATCGACCG CAGCGACGGG GTCTGTGCTT 
TCGGCCTCTC CTCCAAACTC TTATATTCAG CAGCTGCAAC AGCCACAACC AATGCCGATC 
CTCCCTGTGA GTCACTCGAT ACCGGCAGTT GAGGTTCATC GTACTCCAGT GCACCGGGCA 
GCACCTTCAA ACAATCTGCT AATGAGCAGT ACCGCCACGA GTGATTTTAA TACCCTTCCG 
ATGGTGTTGC CACGTGTCTC AACGCTACCA TCACCGACTG GAGTAGCAGA ATCATCGATC 
ACGGGGCTTG TGGAGCCTGC CAATCTACTC CTTTCGTCAC CTGCACGAGA TGCTGTAACG 
CAGCCGCAGC CAGCGGTGTC CGCGCCTCCA TCAGTTGCGC CAGCTGCATC GAACATAATT 
GAACTTCCCG GAATCGTAGG CACATCTTGT CCACCACCAC CAGCACCACC GCAACAGTTG 
CCAACGGAGC GACGGGAAGA ACAGGGGCAG AGTGAAACAG GAAGCGAACG CGCTCCAGTC 
GTCACCGATG TTGAGACGGG CAACGTTCCG ATGAGCCATC TTCTGGCCGG TTCGCAGTAC 
CCTTCGCTAC AGCACGTACC GATCGGCATT GCGCAAAGCT TTGCGCCGAT CTTCGTACAA 
CCGAACCCGG CACCAGCGGA AGCGTCTGAC ATCTACTCGG ACTACATTAA TGATCCCTAC 
AATCTGACAC TGCAAATTGA CAACGGAGCG CCGGGTAGCG CCCCCAGTAG TAGCGTTACT 
GCCCCAGAAC CAGCACCACC GCAATCGGCA ACCGCCCAAC CGGACACGTT GTCAAGGACG 
CTAGAGGACG CGAAAACGGG TGGCCCGATG CTGACGGGCT CGACGACAGT GACAGCGACG 
CCGTCTGCCC AGCTGGCGAA CGTGTTTCAG TCGGCGCAAT ACTTTAGCTT CCCGGCCAGT 
GGCCACATAC CGCCCGGCTC GGAGATGCTT TTCGGGGAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Anopheles gambiae genome: an update.
Trends in parasitology20(2)49-52(2004 Feb)
Mongin E,Louis C,Holt RA,Birney E,Collins FH

The genome sequence of the malaria mosquito Anopheles gambiae.
Science (New York, N.Y.)298(5591)129-49(2002 Oct)
Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL

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AgaP_AGAP001527 cDNA ORF clone, Anopheles gambiae str. PEST

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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