garz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	garz
Entrez Gene ID	36337
Full Name	gartenzwerg
Synonyms	ARF1GEF,ArfGEF,CG 8487,CG8487,Dmel\CG8487,GBF1,GEF,Garz,ang
General protein information	Preferred Names gartenzwerg Names CG8487 gene product from transcript CG8487-RB CG8487-PA CG8487-PB CG8487-PC angina angle wing garz-PA garz-PB garz-PC sec7-family GEF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48F8-48F10\|2-10.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_165881.2	NP_725133.1	gartenzwerg, isoform A
NM_001259349.2	NP_001246278.1	gartenzwerg, isoform C
NM_136917.4	NP_610761.2	gartenzwerg, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

garz interacts genetically with the ARF1 homolog ARF79F and with the ARF1-GAP homolog Gap69C, thus placing garz in a regulatory circuit that controls coatomer protein complex I trafficking in Drosophila.
Title: The Drosophila Sec7 domain guanine nucleotide exchange factor protein Gartenzwerg localizes at the cis-Golgi and is essential for epithelial tube expansion.

In the absence of functional Garz protein, the integrity of the Golgi complex is impaired and as a result, both vesicle transport of cargo proteins and directed apical membrane delivery are severely disrupted.
Title: GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis.

The following garz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the garz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09721	NM_136917.4 Latest version!	Drosophila melanogaster gartenzwerg (garz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa09722	NM_165881.2 Latest version!	Drosophila melanogaster gartenzwerg (garz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12937	NM_001259349.2 Latest version!	Drosophila melanogaster gartenzwerg (garz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

garz ( NM_136917.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610761.2 Drosophila melanogaster gartenzwerg (garz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09721
Clone ID Related Accession (Same CDS sequence)	NM_136917.4
Accession Version	NM_136917.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gartenzwerg, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136917.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGGCAA GCGAGCTCAG ACCTTAACGA ACCCAGCTCC ACAGGCAGCT 
GTGGCTGCCG CTCCACAGCA ACAGTTACCA GCGGTGACCA TTTTGCCCAG GCAAACCCAG 
GTTTCCAACG AGTTAGTGGT GAGCGCGCCT ACTCCGCCGG CAGCCACACC TTTGCTGGGC 
TCTCCCGTCG AGTCGCCGAG GCGGAGCATA ATACTGCAGC CACCCATGGC CGATGTACTG 
CAACAGCCGC CCAGCTTTGT ATTTGCTCAG CCCATTATTG TGCCACCCCA GCCGCCTGCA 
GTTACGGACC CAATACCACC AAGTACATTA TTGCCGGATT TGGTGAATGA GGCAACTGCT 
GCTGCCGTGC AAGCCACGAC CACGTCCCCG ACGCACAGCC CGCAGGAGGC GGAGCAGCCG 
GCTTCAATAG TGCAGCAGAC CAACATCGTA ACCACCAACA ATACGTACAA TAGCTACGCC 
ATTGAGGTGC CCATGGCGCC GGAGACAACT GCGGAACAGT TTGGGCAGCA GCAGCAGCAA 
CTGCTTTACC AACAGTACTA TCAACAGTAT CAGGCCCAGC AGCAACAGTT GCCGGCTCCA 
GCCAGCGACC CTGCCATCAA TGTGCCAATT AGTCATCTGC TGGCCGGAAA TGCGTACCCC 
TCGCTCCCCA AAATGCCGCA GGCATCCATT GTGCACAGCT TTGCGCCCGT TTACGAAAGC 
CAGGCGGCGA CGAGTGGAGC TGGGACAGCA GCAGCGGACA TCTATCAGGA GTATGTGCAA 
AATCCGTACA ACCTTACGTT GCAACAGCAT CCCCAACAGC AGCTCCATCA GCAGCAGCAG 
CAGCAACAAC AGCAGGCTAC AGGAATGGCC AACGCATTTC CCGCCGTTGC CACGCCAGCC 
AACTACTTTA ATGTGAATGT GGACCCCAGT AGCATACCGC CCGGATCGGA ACTGCTCTAC 
GGCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610761.2
CDS	315..6266
Translation	MALPGNGIYVVRGEMATLMTAMRRGTRWNATAYVDDENDSLLKLFIDLKHELNRIEDLRQIEPQVFLAPFLEVIRTADATGPLTSLALASVNKLLSYGLIDPTSPNLADIVERIADAVTHARFMGTDQSSDGVTFMRVIEVLHTLIRSPEGAAVSNVSMCEVMLSCFKISFEPRLSELLRRSAEKSLKDMVLLFFMRLPQFAEERSDTMLQKRFTIGDAASGATQEKLKRKTVAQAQTAPRKSSAVEEPPQTPQSANLTVPGHLKAPILATTPASPAGNILDMQGKITQTPTTTASTGEDETTVPETPVIQVESTESEPLLDGETGEATSTLAEANSSEYINSVGVRFTQQSTDHDVTSLSPYGLPFIQELFRFLIILCNPLDKQNSDSMMHTGLSLLTVAFEVAADNIGKYEGLLELVKDDLCRNLISLLSSERLSIFAADLQLCFLLFESLRGHLKFQLEAYLRKLSEIIASDNPKTPYEMRELALDNLLQLWRIPGFVTELYINYDCDLYCTDMFESLTNLLSKYTLSATNAVYSTHIISMDTLLSVIDSIERNCAASKNSSNNRESLPEAAPATGGSRHSRHNSGLEGIVIDSGNSVAAEEKVENIASFINASSHRLRLQSGGEGVGITSEQLAKVKQKKRLLSQGTERFNQRPEKGIQYLQEHGILNAELDPMQVALFLRENPGLDKKMIGEYISKKKNVDSKILINFVDSFDFTGLRVDQALRLYLETFRLPGEAPLIFLVLEHFSDHWHKQNQDPFANVDAAFRLAYAIIMLNMDQHNSNAKRLNVPMTLEDFTKNLRGLNGGEDFDQEMLAQVFNAIKNEEIVMPAEQTGLVRENYQWKVLLRRGDTHDGHFHYVHDASYDVEIFNIVWGASLSALSFMFDKSTETGYQRTLAGFSKSAAISAHYNLHSDFDALVLTLCKFTTLLSSVEQHEPAPANNETQQAVNFGLNGKAQAAMRTVFLLVHDYGDCLRESWKHILDLYLQLFRLKLLPKSLIEVEDFCEANGKAMLILEKPREKQESGLFSSLYSFISSEGQREPTYEEQDFIKLGRKCIKECQLDQMLQESKFVQLESLQELLKCVLALLKAPQGHKSIGLPYAEDQTVFWMEFLVKIVVHNRDRMIPLWPAVRDQMYLLLMGSASCGYDYLLNRCIVAVLKLAIYLMRNEELCPIVLQSLKMLLMLKPALLLRISKQISIGIYELLKTSAQNIHSEQDWQIIFNLLECVGAGAVPPNYDDAQLPLPPNGSAKSDGAISGEEDATAVPERGYTSDSEITKASAAPAVSSPSAENWILVNNKDSELTTASRPQSPPSLSAPPVNTLVYNCQLLDHAPFALFKCWDSLAFIVRSVAHITPYNFEACVRCIRIFVEACRDGGIRQRRKLESAAKQKSSKKRSERKPGMASSASSSNLTLLTGDPSDNQINGNAAEQEDLAQRYEQLSIQLLDLMYTLYTRTAQIFRWWAEEGCTVPQSAALWSPGWCPLLQGIARLAMDRRREVRTHAISCLQQRALLVHDLQTLSGTEWCSCFHQVLFPLLNELLPESNAAGQLDAALLEESRIRTATIMSKVFLQHLTTLIELGNAFNELWLDILDYIERFMKVGSDTLSEQMQEILKNMLLVMHSVRVFHNQDGSLQQALWELTWRRIGEFLPNLKEELFHDEGKRAQTLTNPAPQAAVAAAPQQQLPAVTILPRQTQVSNELVVSAPTPPAATPLLGSPVESPRRSIILQPPMADVLQQPPSFVFAQPIIVPPQPPAVTDPIPPSTLLPDLVNEATAAAVQATTTSPTHSPQEAEQPASIVQQTNIVTTNNTYNSYAIEVPMAPETTAEQFGQQQQQLLYQQYYQQYQAQQQQLPAPASDPAINVPISHLLAGNAYPSLPKMPQASIVHSFAPVYESQAATSGAGTAAADIYQEYVQNPYNLTLQQHPQQQLHQQQQQQQQQATGMANAFPAVATPANYFNVNVDPSSIPPGSELLYGQQ

Target ORF information:

RefSeq Version	NM_136917.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gartenzwerg (garz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136917.4

ORF Insert Sequence:

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGGCAA GCGAGCTCAG ACCTTAACGA ACCCAGCTCC ACAGGCAGCT 
GTGGCTGCCG CTCCACAGCA ACAGTTACCA GCGGTGACCA TTTTGCCCAG GCAAACCCAG 
GTTTCCAACG AGTTAGTGGT GAGCGCGCCT ACTCCGCCGG CAGCCACACC TTTGCTGGGC 
TCTCCCGTCG AGTCGCCGAG GCGGAGCATA ATACTGCAGC CACCCATGGC CGATGTACTG 
CAACAGCCGC CCAGCTTTGT ATTTGCTCAG CCCATTATTG TGCCACCCCA GCCGCCTGCA 
GTTACGGACC CAATACCACC AAGTACATTA TTGCCGGATT TGGTGAATGA GGCAACTGCT 
GCTGCCGTGC AAGCCACGAC CACGTCCCCG ACGCACAGCC CGCAGGAGGC GGAGCAGCCG 
GCTTCAATAG TGCAGCAGAC CAACATCGTA ACCACCAACA ATACGTACAA TAGCTACGCC 
ATTGAGGTGC CCATGGCGCC GGAGACAACT GCGGAACAGT TTGGGCAGCA GCAGCAGCAA 
CTGCTTTACC AACAGTACTA TCAACAGTAT CAGGCCCAGC AGCAACAGTT GCCGGCTCCA 
GCCAGCGACC CTGCCATCAA TGTGCCAATT AGTCATCTGC TGGCCGGAAA TGCGTACCCC 
TCGCTCCCCA AAATGCCGCA GGCATCCATT GTGCACAGCT TTGCGCCCGT TTACGAAAGC 
CAGGCGGCGA CGAGTGGAGC TGGGACAGCA GCAGCGGACA TCTATCAGGA GTATGTGCAA 
AATCCGTACA ACCTTACGTT GCAACAGCAT CCCCAACAGC AGCTCCATCA GCAGCAGCAG 
CAGCAACAAC AGCAGGCTAC AGGAATGGCC AACGCATTTC CCGCCGTTGC CACGCCAGCC 
AACTACTTTA ATGTGAATGT GGACCCCAGT AGCATACCGC CCGGATCGGA ACTGCTCTAC 
GGCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

garz ( NM_165881.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725133.1 Drosophila melanogaster gartenzwerg (garz), transcript variant A, mRNA.

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CloneID	OFa09722
Clone ID Related Accession (Same CDS sequence)	NM_165881.2
Accession Version	NM_165881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gartenzwerg, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165881.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGATTC AATTACTCCA GCGGTTTCGC TTGACTATAC GCAAATTAGA 
TTCGCCCATT CTACGGGCAC AACCGATTAT TCCTTGTCCC TGCCGTTATA TGGCTATAGT 
CCAAAGATTT CCTCAATATC CGCAAGCAAA TGTGATACCC TTCCGGCTAC GCAGCGCAAG 
AAACCATGGG CCAAGCTAAG ATCCAAGCTA AAGTTGCTCA AGAAAGTTGG CAAAAAGAAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725133.1
CDS	315..5537
Translation	MALPGNGIYVVRGEMATLMTAMRRGTRWNATAYVDDENDSLLKLFIDLKHELNRIEDLRQIEPQVFLAPFLEVIRTADATGPLTSLALASVNKLLSYGLIDPTSPNLADIVERIADAVTHARFMGTDQSSDGVTFMRVIEVLHTLIRSPEGAAVSNVSMCEVMLSCFKISFEPRLSELLRRSAEKSLKDMVLLFFMRLPQFAEERSDTMLQKRFTIGDAASGATQEKLKRKTVAQAQTAPRKSSAVEEPPQTPQSANLTVPGHLKAPILATTPASPAGNILDMQGKITQTPTTTASTGEDETTVPETPVIQVESTESEPLLDGETGEATSTLAEANSSEYINSVGVRFTQQSTDHDVTSLSPYGLPFIQELFRFLIILCNPLDKQNSDSMMHTGLSLLTVAFEVAADNIGKYEGLLELVKDDLCRNLISLLSSERLSIFAADLQLCFLLFESLRGHLKFQLEAYLRKLSEIIASDNPKTPYEMRELALDNLLQLWRIPGFVTELYINYDCDLYCTDMFESLTNLLSKYTLSATNAVYSTHIISMDTLLSVIDSIERNCAASKNSSNNRESLPEAAPATGGSRHSRHNSGLEGIVIDSGNSVAAEEKVENIASFINASSHRLRLQSGGEGVGITSEQLAKVKQKKRLLSQGTERFNQRPEKGIQYLQEHGILNAELDPMQVALFLRENPGLDKKMIGEYISKKKNVDSKILINFVDSFDFTGLRVDQALRLYLETFRLPGEAPLIFLVLEHFSDHWHKQNQDPFANVDAAFRLAYAIIMLNMDQHNSNAKRLNVPMTLEDFTKNLRGLNGGEDFDQEMLAQVFNAIKNEEIVMPAEQTGLVRENYQWKVLLRRGDTHDGHFHYVHDASYDVEIFNIVWGASLSALSFMFDKSTETGYQRTLAGFSKSAAISAHYNLHSDFDALVLTLCKFTTLLSSVEQHEPAPANNETQQAVNFGLNGKAQAAMRTVFLLVHDYGDCLRESWKHILDLYLQLFRLKLLPKSLIEVEDFCEANGKAMLILEKPREKQESGLFSSLYSFISSEGQREPTYEEQDFIKLGRKCIKECQLDQMLQESKFVQLESLQELLKCVLALLKAPQGHKSIGLPYAEDQTVFWMEFLVKIVVHNRDRMIPLWPAVRDQMYLLLMGSASCGYDYLLNRCIVAVLKLAIYLMRNEELCPIVLQSLKMLLMLKPALLLRISKQISIGIYELLKTSAQNIHSEQDWQIIFNLLECVGAGAVPPNYDDAQLPLPPNGSAKSDGAISGEEDATAVPERGYTSDSEITKASAAPAVSSPSAENWILVNNKDSELTTASRPQSPPSLSAPPVNTLVYNCQLLDHAPFALFKCWDSLAFIVRSVAHITPYNFEACVRCIRIFVEACRDGGIRQRRKLESAAKQKSSKKRSERKPGMASSASSSNLTLLTGDPSDNQINGNAAEQEDLAQRYEQLSIQLLDLMYTLYTRTAQIFRWWAEEGCTVPQSAALWSPGWCPLLQGIARLAMDRRREVRTHAISCLQQRALLVHDLQTLSGTEWCSCFHQVLFPLLNELLPESNAAGQLDAALLEESRIRTATIMSKVFLQHLTTLIELGNAFNELWLDILDYIERFMKVGSDTLSEQMQEILKNMLLVMHSVRVFHNQDGSLQQALWELTWRRIGEFLPNLKEELFHDEDSITPAVSLDYTQIRFAHSTGTTDYSLSLPLYGYSPKISSISASKCDTLPATQRKKPWAKLRSKLKLLKKVGKKN

Target ORF information:

RefSeq Version	NM_165881.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gartenzwerg (garz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165881.2

ORF Insert Sequence:

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGATTC AATTACTCCA GCGGTTTCGC TTGACTATAC GCAAATTAGA 
TTCGCCCATT CTACGGGCAC AACCGATTAT TCCTTGTCCC TGCCGTTATA TGGCTATAGT 
CCAAAGATTT CCTCAATATC CGCAAGCAAA TGTGATACCC TTCCGGCTAC GCAGCGCAAG 
AAACCATGGG CCAAGCTAAG ATCCAAGCTA AAGTTGCTCA AGAAAGTTGG CAAAAAGAAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

garz ( NM_001259349.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246278.1 Drosophila melanogaster gartenzwerg (garz), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12937
Clone ID Related Accession (Same CDS sequence)	NM_001259349.2
Accession Version	NM_001259349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gartenzwerg, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259349.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGCAGA TTCAATTACT CCAGCGGTTT CGCTTGACTA TACGCAAATT 
AGATTCGCCC ATTCTACGGG CACAACCGAT TATTCCTTGT CCCTGCCGTT ATATGGCTAT 
AGTCCAAAGA TTTCCTCAAT ATCCGCAAGC AAATGTGATA CCCTTCCGGC TACGCAGCGC 
AAGAAACCAT GGGCCAAGCT AAGATCCAAG CTAAAGTTGC TCAAGAAAGT TGGCAAAAAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246278.1
CDS	315..5540
Translation	MALPGNGIYVVRGEMATLMTAMRRGTRWNATAYVDDENDSLLKLFIDLKHELNRIEDLRQIEPQVFLAPFLEVIRTADATGPLTSLALASVNKLLSYGLIDPTSPNLADIVERIADAVTHARFMGTDQSSDGVTFMRVIEVLHTLIRSPEGAAVSNVSMCEVMLSCFKISFEPRLSELLRRSAEKSLKDMVLLFFMRLPQFAEERSDTMLQKRFTIGDAASGATQEKLKRKTVAQAQTAPRKSSAVEEPPQTPQSANLTVPGHLKAPILATTPASPAGNILDMQGKITQTPTTTASTGEDETTVPETPVIQVESTESEPLLDGETGEATSTLAEANSSEYINSVGVRFTQQSTDHDVTSLSPYGLPFIQELFRFLIILCNPLDKQNSDSMMHTGLSLLTVAFEVAADNIGKYEGLLELVKDDLCRNLISLLSSERLSIFAADLQLCFLLFESLRGHLKFQLEAYLRKLSEIIASDNPKTPYEMRELALDNLLQLWRIPGFVTELYINYDCDLYCTDMFESLTNLLSKYTLSATNAVYSTHIISMDTLLSVIDSIERNCAASKNSSNNRESLPEAAPATGGSRHSRHNSGLEGIVIDSGNSVAAEEKVENIASFINASSHRLRLQSGGEGVGITSEQLAKVKQKKRLLSQGTERFNQRPEKGIQYLQEHGILNAELDPMQVALFLRENPGLDKKMIGEYISKKKNVDSKILINFVDSFDFTGLRVDQALRLYLETFRLPGEAPLIFLVLEHFSDHWHKQNQDPFANVDAAFRLAYAIIMLNMDQHNSNAKRLNVPMTLEDFTKNLRGLNGGEDFDQEMLAQVFNAIKNEEIVMPAEQTGLVRENYQWKVLLRRGDTHDGHFHYVHDASYDVEIFNIVWGASLSALSFMFDKSTETGYQRTLAGFSKSAAISAHYNLHSDFDALVLTLCKFTTLLSSVEQHEPAPANNETQQAVNFGLNGKAQAAMRTVFLLVHDYGDCLRESWKHILDLYLQLFRLKLLPKSLIEVEDFCEANGKAMLILEKPREKQESGLFSSLYSFISSEGQREPTYEEQDFIKLGRKCIKECQLDQMLQESKFVQLESLQELLKCVLALLKAPQGHKSIGLPYAEDQTVFWMEFLVKIVVHNRDRMIPLWPAVRDQMYLLLMGSASCGYDYLLNRCIVAVLKLAIYLMRNEELCPIVLQSLKMLLMLKPALLLRISKQISIGIYELLKTSAQNIHSEQDWQIIFNLLECVGAGAVPPNYDDAQLPLPPNGSAKSDGAISGEEDATAVPERGYTSDSEITKASAAPAVSSPSAENWILVNNKDSELTTASRPQSPPSLSAPPVNTLVYNCQLLDHAPFALFKCWDSLAFIVRSVAHITPYNFEACVRCIRIFVEACRDGGIRQRRKLESAAKQKSSKKRSERKPGMASSASSSNLTLLTGDPSDNQINGNAAEQEDLAQRYEQLSIQLLDLMYTLYTRTAQIFRWWAEEGCTVPQSAALWSPGWCPLLQGIARLAMDRRREVRTHAISCLQQRALLVHDLQTLSGTEWCSCFHQVLFPLLNELLPESNAAGQLDAALLEESRIRTATIMSKVFLQHLTTLIELGNAFNELWLDILDYIERFMKVGSDTLSEQMQEILKNMLLVMHSVRVFHNQDGSLQQALWELTWRRIGEFLPNLKEELFHDEADSITPAVSLDYTQIRFAHSTGTTDYSLSLPLYGYSPKISSISASKCDTLPATQRKKPWAKLRSKLKLLKKVGKKN

Target ORF information:

RefSeq Version	NM_001259349.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gartenzwerg (garz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259349.2

ORF Insert Sequence:

ATGGCGCTTC CAGGCAACGG CATCTACGTG GTGCGGGGCG AAATGGCCAC CCTGATGACG 
GCGATGCGAC GTGGAACGCG TTGGAATGCC ACCGCCTACG TGGACGACGA GAATGACTCG 
CTGCTGAAGC TGTTCATTGA CCTCAAGCAT GAGCTAAATC GGATCGAGGA CCTGCGTCAG 
ATCGAGCCTC AGGTCTTCCT GGCTCCGTTT CTTGAGGTGA TTCGCACGGC GGACGCCACG 
GGTCCGTTGA CTAGTCTAGC CTTGGCCTCG GTTAACAAAT TATTGTCTTA CGGGCTAATA 
GATCCCACGT CTCCAAATCT GGCTGACATT GTGGAGCGCA TTGCCGATGC TGTGACACAT 
GCCCGCTTTA TGGGCACCGA TCAGTCCTCG GATGGTGTCA CCTTTATGCG AGTGATTGAG 
GTGCTGCACA CGCTTATCCG CAGTCCCGAA GGAGCCGCCG TCAGCAATGT GTCCATGTGC 
GAGGTGATGC TCAGCTGCTT CAAGATCTCC TTCGAGCCGA GGCTGAGTGA ACTGCTTCGT 
CGCTCGGCGG AAAAATCGCT CAAGGACATG GTGCTGCTCT TCTTCATGCG CCTTCCCCAG 
TTCGCCGAAG AGCGAAGTGA CACCATGCTC CAGAAGCGAT TTACTATCGG CGATGCTGCC 
AGCGGAGCTA CCCAAGAAAA ACTAAAGCGT AAGACGGTTG CCCAAGCCCA GACAGCACCC 
AGGAAATCGT CAGCAGTGGA GGAACCGCCT CAAACACCGC AGTCTGCCAA CCTGACGGTG 
CCAGGGCACT TGAAGGCACC CATATTGGCC ACCACACCCG CCAGTCCAGC GGGAAACATA 
TTGGACATGC AGGGCAAGAT TACGCAGACA CCAACAACAA CGGCGAGCAC GGGGGAAGAT 
GAAACCACTG TCCCGGAGAC TCCTGTTATT CAAGTGGAGT CGACTGAATC GGAGCCCTTG 
CTGGACGGCG AAACGGGTGA AGCAACCAGC ACCCTGGCCG AGGCAAATAG TAGCGAGTAC 
ATCAACTCGG TGGGCGTCCG TTTTACACAG CAGTCCACCG ATCACGATGT AACATCACTA 
TCCCCTTATG GCCTGCCCTT CATCCAGGAG TTGTTCCGAT TCCTCATAAT CCTCTGCAAT 
CCTCTGGATA AGCAGAACTC GGATAGCATG ATGCACACGG GTCTTAGTCT ACTTACTGTA 
GCTTTTGAAG TCGCAGCCGA TAATATTGGA AAGTATGAGG GCTTGCTGGA GCTGGTTAAG 
GACGACTTAT GCAGAAACTT AATATCGCTT CTCAGCTCAG AGCGGCTTAG CATCTTTGCC 
GCCGATTTGC AGCTCTGTTT TTTGCTTTTC GAGTCTCTCC GCGGACATCT CAAGTTTCAG 
CTGGAAGCCT ACCTTAGAAA ATTGAGCGAG ATTATTGCTA GCGATAATCC CAAGACGCCC 
TACGAAATGC GAGAACTCGC TCTGGACAAT CTACTGCAGT TGTGGCGCAT TCCCGGCTTC 
GTCACGGAAT TGTATATCAA CTACGATTGT GACTTGTACT GCACGGATAT GTTTGAAAGT 
TTGACAAACC TACTGAGCAA GTATACGCTG TCAGCAACGA ATGCAGTTTA TAGCACCCAC 
ATTATCTCAA TGGACACCCT GTTAAGTGTG ATAGACAGTA TCGAGCGAAA TTGTGCCGCG 
AGCAAGAATA GCAGCAACAA CAGAGAGTCC TTGCCAGAAG CTGCCCCAGC AACAGGTGGC 
AGCCGCCATT CTCGCCACAA CAGCGGATTG GAGGGAATCG TAATTGATTC TGGCAATAGT 
GTAGCTGCAG AAGAGAAAGT GGAGAACATC GCAAGCTTTA TAAATGCGAG CTCACATCGA 
CTACGACTAC AATCTGGCGG AGAGGGAGTG GGAATAACCA GTGAACAGCT GGCCAAGGTC 
AAACAGAAGA AGCGTCTGCT ATCCCAAGGC ACAGAGCGAT TTAATCAGCG TCCAGAGAAA 
GGAATCCAGT ATCTGCAAGA ACACGGCATC CTAAATGCCG AGCTTGATCC CATGCAGGTG 
GCCCTGTTCC TTCGAGAAAA TCCCGGGCTC GATAAAAAAA TGATTGGCGA ATATATCTCG 
AAAAAGAAAA ACGTCGACTC TAAGATTCTA ATTAATTTTG TGGACTCGTT TGATTTCACT 
GGTCTTCGAG TGGATCAAGC ATTGCGTCTT TATCTGGAGA CCTTCAGATT GCCCGGAGAG 
GCTCCATTGA TCTTTTTGGT GCTGGAACAC TTTTCTGATC ATTGGCATAA ACAAAACCAA 
GATCCGTTTG CCAACGTAGA CGCTGCTTTT CGCTTGGCCT ATGCCATCAT CATGCTGAAC 
ATGGATCAGC ACAACTCGAA CGCGAAGCGT TTAAATGTTC CAATGACGCT CGAGGACTTC 
ACTAAGAATT TGCGTGGTCT AAACGGTGGC GAAGATTTCG ATCAAGAAAT GCTGGCTCAA 
GTCTTTAATG CAATCAAGAA CGAAGAGATC GTTATGCCAG CAGAGCAAAC GGGTCTGGTG 
CGTGAAAATT ATCAATGGAA AGTACTGCTT CGACGAGGAG ACACGCACGA TGGACATTTT 
CACTATGTGC ATGACGCATC ATACGACGTG GAGATCTTCA ATATTGTGTG GGGTGCTTCT 
CTGAGCGCCC TAAGCTTTAT GTTTGATAAA AGCACTGAAA CGGGCTACCA AAGAACTCTA 
GCAGGTTTCA GCAAATCCGC TGCCATATCG GCGCACTATA ATCTGCATTC GGACTTCGAT 
GCCCTCGTTT TAACTCTCTG CAAATTCACA ACGCTGCTGA GCAGCGTAGA ACAGCATGAG 
CCCGCTCCGG CGAACAATGA AACCCAGCAA GCTGTGAACT TTGGATTGAA CGGAAAGGCT 
CAGGCTGCCA TGCGAACGGT GTTTCTATTG GTTCACGACT ACGGCGATTG CTTAAGAGAG 
AGCTGGAAAC ACATTTTGGA CCTATATCTG CAGCTTTTCC GTCTAAAGTT GCTGCCAAAA 
TCATTGATCG AAGTGGAAGA CTTTTGTGAG GCGAACGGAA AGGCCATGTT AATCCTGGAA 
AAGCCCCGCG AGAAGCAGGA ATCGGGACTA TTTTCCAGCC TGTACTCATT TATCAGCTCG 
GAGGGTCAGC GAGAACCAAC GTACGAGGAG CAGGACTTCA TCAAACTGGG ACGGAAGTGC 
ATTAAGGAGT GCCAGCTGGA TCAAATGCTG CAGGAATCAA AGTTTGTGCA ACTAGAGTCG 
CTGCAGGAGT TGCTTAAATG CGTTCTAGCG CTACTGAAGG CTCCTCAGGG GCACAAATCC 
ATTGGCCTGC CGTACGCCGA AGATCAAACT GTTTTCTGGA TGGAATTTTT GGTCAAGATA 
GTTGTTCATA ACCGGGATCG CATGATACCG CTGTGGCCAG CAGTTCGAGA CCAAATGTAC 
CTACTGCTTA TGGGCAGTGC CTCCTGTGGA TACGACTACC TACTCAACCG ATGCATTGTA 
GCGGTCCTAA AACTAGCTAT CTATCTGATG CGAAACGAAG AACTGTGTCC GATCGTATTG 
CAATCGCTCA AGATGCTTTT AATGCTTAAG CCAGCCTTGT TGCTGCGCAT TTCTAAACAG 
ATTTCCATTG GTATCTATGA GCTGCTCAAG ACGTCGGCCC AAAATATTCA TTCCGAGCAG 
GACTGGCAGA TTATTTTCAA TCTACTTGAA TGCGTGGGAG CCGGTGCTGT GCCGCCCAAT 
TATGATGATG CCCAGCTGCC ATTGCCGCCC AACGGAAGTG CAAAGTCTGA TGGCGCTATA 
AGTGGCGAAG AGGACGCAAC TGCCGTGCCA GAGCGTGGTT ACACTTCGGA TTCGGAGATC 
ACGAAAGCAT CTGCAGCACC TGCAGTCTCC AGTCCAAGTG CTGAGAACTG GATTCTGGTC 
AATAACAAGG ACAGTGAATT GACTACGGCC TCTAGACCAC AATCTCCGCC TAGCCTGAGT 
GCTCCTCCAG TAAATACGCT TGTGTACAAT TGCCAGCTAC TAGACCACGC TCCGTTTGCT 
CTTTTCAAGT GCTGGGATTC GCTGGCGTTT ATCGTGCGCA GTGTGGCACA CATCACGCCT 
TACAATTTTG AAGCCTGCGT TCGCTGCATC CGCATCTTTG TGGAGGCTTG TCGGGATGGA 
GGTATACGCC AGCGGCGAAA GCTGGAATCG GCGGCTAAGC AGAAAAGTTC CAAGAAGCGC 
AGCGAACGCA AACCGGGCAT GGCTTCCTCC GCCTCGAGTA GTAATCTTAC TCTTCTGACG 
GGCGACCCGT CCGACAACCA GATAAACGGA AATGCGGCAG AGCAGGAGGA CCTGGCCCAG 
CGCTACGAAC AGTTGTCCAT TCAACTGCTG GACCTGATGT ATACGTTGTA CACGCGAACT 
GCCCAAATCT TCCGATGGTG GGCGGAAGAA GGATGCACAG TGCCGCAGTC GGCAGCTTTG 
TGGTCACCGG GCTGGTGTCC ATTGCTTCAG GGAATCGCCA GGCTGGCAAT GGATCGACGG 
CGAGAGGTGC GCACCCATGC CATATCGTGC CTGCAGCAGC GGGCATTGCT AGTCCATGAC 
CTGCAAACGT TGTCGGGAAC GGAGTGGTGC TCTTGCTTCC ACCAGGTGCT GTTCCCCCTC 
CTAAACGAAC TGCTGCCCGA GAGTAATGCA GCCGGCCAAC TGGATGCCGC TCTCCTCGAA 
GAGTCGCGTA TACGAACGGC CACCATTATG TCTAAGGTGT TCCTGCAACA CCTGACCACG 
CTCATCGAGC TGGGAAATGC TTTTAACGAG CTGTGGCTGG ATATATTGGA CTACATTGAG 
CGCTTTATGA AGGTGGGATC GGACACATTG TCCGAGCAGA TGCAGGAGAT ACTGAAGAAC 
ATGCTGCTGG TGATGCATTC AGTGCGAGTG TTCCACAATC AGGATGGTAG TTTACAGCAG 
GCTCTTTGGG AGCTAACCTG GCGACGCATC GGCGAATTTT TGCCCAACCT GAAGGAGGAG 
CTTTTCCACG ACGAAGCAGA TTCAATTACT CCAGCGGTTT CGCTTGACTA TACGCAAATT 
AGATTCGCCC ATTCTACGGG CACAACCGAT TATTCCTTGT CCCTGCCGTT ATATGGCTAT 
AGTCCAAAGA TTTCCTCAAT ATCCGCAAGC AAATGTGATA CCCTTCCGGC TACGCAGCGC 
AAGAAACCAT GGGCCAAGCT AAGATCCAAG CTAAAGTTGC TCAAGAAAGT TGGCAAAAAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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garz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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