Gbf1 cDNA ORF clone, Mus musculus(house mouse)

The following Gbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu55180 XM_006526583.3
Latest version!
Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu07918 NM_178930.3
Latest version!
Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $951.30
$1359.00
OMu55177 XM_006526580.3
Latest version!
Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu55178 XM_006526581.3
Latest version!
Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu55179 XM_006526582.4
Latest version!
Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OMu55179 XM_006526582.3 Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu55177 XM_006526580.2 Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu55180 XM_006526583.2 Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu55178 XM_006526581.2 Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu55180
    Clone ID Related Accession (Same CDS sequence) XM_006526583.2 , XM_006526583.3
    Accession Version XM_006526583.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCAGGA TGTACTTGCC ACTCCAGTGC CAGAGCCATC AGCCTGCTCT GTTCCACACC 
    TTCAGCTTTC AGGGTCTGCG GCTTGATGAA GCTCTCCGAC TCTACCTGGA AGCCTTCCGT
    TTGCCTGGGG AAGCACCCGT TATTCACAGG TTGCTGGAGG TATTCACTGA GCACTGGAGG
    AGTTGTAATG GCTCCCCATT TGCTAATAGT GATGCCTGCT TTGCCCTGGC CTATGCTGTC
    ATCATGCTTA ACACTGACCA ACATAACCAC AATGTTCGAA AGCAGAATGC ACCCATGACC
    CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT GTGAATGGAG GCAAGGACTT TGAGCAAGAC
    ATCCTGGAAG ACATGTACCA TGCCATCAAG AATGAGGAGA TTGTGATGCC TGAGGAGCAG
    ACAGGTCTGG TCCGTGAGAA CTATGTGTGG AGCGTGCTGC TTCACCGAGG TGCTAGCCCT
    GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC AGCTACGATC TTGACCTCTT CACTATGACC
    TGGGGCCCCA CGATCGCTGC TCTCTCTTAT GTCTTCGATA AAAGCCTTGA GGAGACCATC
    ATCCAGAAAG CCATCTCAGG CTTCAGGAAG TGTGCCATGA TCTCCGCCCA CTATGGCCTC
    AGCGATGTGT TTGACAATCT CATCATCTCT CTGTGCAAAT TCACAGCTCT AAGTAGTGAG
    TCTATTGAGA ACCTTCCCAG TGTATTTGGA AGCAACCCTA AAGCCCACAT AGCAGCCAAG
    ACAGTATTCC ATTTGGCCCA TCGTCATGGT GACATCCTTC GGGAGGGCTG GAAGAATATC
    ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC CAACTTTTAC CCAAGGCTAT GGTGGAGGTA
    GAAGATTTTG TGGATCCCAA TGGCAAGATC TCTCTACAGC GGGAGGAGAC ACCATCCAAC
    CGAGGAGAGT CAACAGTTCT TAGCTTTGTA AGCTGGCTGA CATTGAGTGG TCCTGAGCAG
    TCCAGTGTAC GGGGTCCATC TACAGAGAAC CAAGAGGCCA AGAGAGTGGC CTTGGACTGT
    ATCAAGCAAT GTGACCCAGA AAAAATGATC ACAGAAAGCA AGTTCCTCCA GCTGGAATCA
    CTGCAGGAGC TCATGAAGGC TCTGGTCTCA GTGACACCAG ATGAAGAGAC ATATGACGAA
    GAAGATGCTG CTTTCTGTCT AGAGATGCTG CTAAGGATCG TGTTAGAGAA CAGGGACCGT
    GTGGGCTGTG TATGGCAGAC TGTTCGAGAT CATCTATACC ACCTGTGTGT TCAGGCACAA
    GATTTCTGCT TCCTTGTGGA GCGAGCAGTG GTAGGGCTGC TACGCCTAGC CATCCGGCTA
    CTCCGGAGAG AAGAGATTAG TGGCCAGGTC CTGCTCTCTC TGCGCATCTT GTTACTGATG
    AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC CAGGTTGCAT ATGGGCTGCA TGAACTCCTC
    AAGACCAATG CTGCCAACAT CCACTCGGGT GATGACTGGG CCACCCTCTT CACATTGCTG
    GAGTGCATTG GCTCAGGCGT GAAGCCTCCA GATGCTCTTC AAGCCACAGC CAGGGCTGAT
    GCTCCTGATG CGGGAGCACA GTCGGACAGT GAGCTCCCAT CCTACCATCA AAATGATGTC
    AGCCTGGACC GAGGGTACAC TTCCGACTCA GAAGTCTACA CAGACCATGG CAGGCCTGGC
    AAGATACACC GATCGGCCAC AGATGCTGAC ATGGTCAACA GTGGTTGGTT AGTGGTGGGG
    AAGGATGACA TTGATAACTC CAAACCAGGA GCAGGGCTCA GCAGGCCAGG CCCTTCACCC
    CTGGTCAACC AGTACAGCCT TACAGTGGGC CTGGATCTGG GCCCACACGA CACTAAGTCC
    CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC ATTGTCCGAG ATGCTGCTCA CATCACCCCT
    GACAACTTTG AACTCTGCGT CAAGACTCTC CGCATCTTTG TAGAAGCCAG TCTGAATGGT
    GGGTGCAAGT CCCAGGATAA ACGCAGCAAG AGTCATAAGT ATGACAGCAA AGGGAACCGC
    TTCAAGAAAA AACCCAAGGA GGGCTCGATG CTTCGGCGGC CCCGGGGATC CAACCAGCAT
    GCTACTCGGG GCGGGCACAG TGATGAAGAG GAGGATGAAG GTGTGCCTGC CAGCTACCAT
    ACGGTGTCTT TACAGGTCAG TCAGGACTTG CTAGACCTGA TGCATACCCT GCACACACGG
    GCAGCCTCTA TCTACAGCTC ATGGGCAGAG GAGCAACGCC ACCTGGAGGG AGGTGGCCAG
    AAGATTGAAG CTGACTCACG CACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGA
    ATCGCCTGCT TATGCTGTGA CGCCCGGCGC CAGGTACGGA TGCAGGCCCT GACATATCTG
    CAGCGAGCCC TTCTGGTGCA TGACCTACAG AAGCTAGATG CCCTGGAGTG GGAGTCCTGT
    TTTAACAAGG TGCTGTTTCC TCTCCTGACC AAGCTTTTGG AAAATATCAG CCCTGCAGAT
    GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG
    CAGCATCTGT CTCCACTGCT GTCACTGTCC ACCTTTGCTG CCCTGTGGCT CACTATCCTA
    GACTTCATGG ACAAGTACAT GCATGCAGGT TCCAGTGATT TGCTGTCAGA AGCTATCCCC
    GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCGG AGATTTTCCA CAGTGCAGAT
    GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG GAGATTACCT GGGAGCGCAT CGACTGCTTC
    TTGCCACACT TGCGTGACGA GCTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCTGCG
    GAACCGCACA GTCAAAAACC TCTGGCCTCT ACTCACCTGA CTTCTGCTGC TGGTGACCCC
    AGGATGCCTG GCCATCCACC TCTCCCAGAG ATACCCTCAG AAATGGGGGT TTGTGACTCA
    GAGAAGCCCG AGAGTACCCG AGCTCCCAGC AGCAGCTCCC CCGGATCGCC CATGGCCTCT
    AGCCCCAGCA AACTGAGTCC TGCCCAAGAG GGACCTCCCC CACTGACCCA GCCCCCACTG
    ATCCTGCAGC CCTTGACCTC CCCACTGCAG GTGGGGGTCC CGCCCATGAC TCTGCCCATC
    ATCCTCAACC CTGCACTCAT TGAGGCCACC TCCCCAGTGC CTCTCCTAGC CACACCCCGC
    CCTACAGACC CCATCCCCAC TTCTGAAGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526646.1
    CDS24..3359
    Translation

    Target ORF information:

    RefSeq Version XM_006526583.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCAGGA TGTACTTGCC ACTCCAGTGC CAGAGCCATC AGCCTGCTCT GTTCCACACC 
    TTCAGCTTTC AGGGTCTGCG GCTTGATGAA GCTCTCCGAC TCTACCTGGA AGCCTTCCGT
    TTGCCTGGGG AAGCACCCGT TATTCACAGG TTGCTGGAGG TATTCACTGA GCACTGGAGG
    AGTTGTAATG GCTCCCCATT TGCTAATAGT GATGCCTGCT TTGCCCTGGC CTATGCTGTC
    ATCATGCTTA ACACTGACCA ACATAACCAC AATGTTCGAA AGCAGAATGC ACCCATGACC
    CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT GTGAATGGAG GCAAGGACTT TGAGCAAGAC
    ATCCTGGAAG ACATGTACCA TGCCATCAAG AATGAGGAGA TTGTGATGCC TGAGGAGCAG
    ACAGGTCTGG TCCGTGAGAA CTATGTGTGG AGCGTGCTGC TTCACCGAGG TGCTAGCCCT
    GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC AGCTACGATC TTGACCTCTT CACTATGACC
    TGGGGCCCCA CGATCGCTGC TCTCTCTTAT GTCTTCGATA AAAGCCTTGA GGAGACCATC
    ATCCAGAAAG CCATCTCAGG CTTCAGGAAG TGTGCCATGA TCTCCGCCCA CTATGGCCTC
    AGCGATGTGT TTGACAATCT CATCATCTCT CTGTGCAAAT TCACAGCTCT AAGTAGTGAG
    TCTATTGAGA ACCTTCCCAG TGTATTTGGA AGCAACCCTA AAGCCCACAT AGCAGCCAAG
    ACAGTATTCC ATTTGGCCCA TCGTCATGGT GACATCCTTC GGGAGGGCTG GAAGAATATC
    ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC CAACTTTTAC CCAAGGCTAT GGTGGAGGTA
    GAAGATTTTG TGGATCCCAA TGGCAAGATC TCTCTACAGC GGGAGGAGAC ACCATCCAAC
    CGAGGAGAGT CAACAGTTCT TAGCTTTGTA AGCTGGCTGA CATTGAGTGG TCCTGAGCAG
    TCCAGTGTAC GGGGTCCATC TACAGAGAAC CAAGAGGCCA AGAGAGTGGC CTTGGACTGT
    ATCAAGCAAT GTGACCCAGA AAAAATGATC ACAGAAAGCA AGTTCCTCCA GCTGGAATCA
    CTGCAGGAGC TCATGAAGGC TCTGGTCTCA GTGACACCAG ATGAAGAGAC ATATGACGAA
    GAAGATGCTG CTTTCTGTCT AGAGATGCTG CTAAGGATCG TGTTAGAGAA CAGGGACCGT
    GTGGGCTGTG TATGGCAGAC TGTTCGAGAT CATCTATACC ACCTGTGTGT TCAGGCACAA
    GATTTCTGCT TCCTTGTGGA GCGAGCAGTG GTAGGGCTGC TACGCCTAGC CATCCGGCTA
    CTCCGGAGAG AAGAGATTAG TGGCCAGGTC CTGCTCTCTC TGCGCATCTT GTTACTGATG
    AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC CAGGTTGCAT ATGGGCTGCA TGAACTCCTC
    AAGACCAATG CTGCCAACAT CCACTCGGGT GATGACTGGG CCACCCTCTT CACATTGCTG
    GAGTGCATTG GCTCAGGCGT GAAGCCTCCA GATGCTCTTC AAGCCACAGC CAGGGCTGAT
    GCTCCTGATG CGGGAGCACA GTCGGACAGT GAGCTCCCAT CCTACCATCA AAATGATGTC
    AGCCTGGACC GAGGGTACAC TTCCGACTCA GAAGTCTACA CAGACCATGG CAGGCCTGGC
    AAGATACACC GATCGGCCAC AGATGCTGAC ATGGTCAACA GTGGTTGGTT AGTGGTGGGG
    AAGGATGACA TTGATAACTC CAAACCAGGA GCAGGGCTCA GCAGGCCAGG CCCTTCACCC
    CTGGTCAACC AGTACAGCCT TACAGTGGGC CTGGATCTGG GCCCACACGA CACTAAGTCC
    CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC ATTGTCCGAG ATGCTGCTCA CATCACCCCT
    GACAACTTTG AACTCTGCGT CAAGACTCTC CGCATCTTTG TAGAAGCCAG TCTGAATGGT
    GGGTGCAAGT CCCAGGATAA ACGCAGCAAG AGTCATAAGT ATGACAGCAA AGGGAACCGC
    TTCAAGAAAA AACCCAAGGA GGGCTCGATG CTTCGGCGGC CCCGGGGATC CAACCAGCAT
    GCTACTCGGG GCGGGCACAG TGATGAAGAG GAGGATGAAG GTGTGCCTGC CAGCTACCAT
    ACGGTGTCTT TACAGGTCAG TCAGGACTTG CTAGACCTGA TGCATACCCT GCACACACGG
    GCAGCCTCTA TCTACAGCTC ATGGGCAGAG GAGCAACGCC ACCTGGAGGG AGGTGGCCAG
    AAGATTGAAG CTGACTCACG CACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGA
    ATCGCCTGCT TATGCTGTGA CGCCCGGCGC CAGGTACGGA TGCAGGCCCT GACATATCTG
    CAGCGAGCCC TTCTGGTGCA TGACCTACAG AAGCTAGATG CCCTGGAGTG GGAGTCCTGT
    TTTAACAAGG TGCTGTTTCC TCTCCTGACC AAGCTTTTGG AAAATATCAG CCCTGCAGAT
    GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG
    CAGCATCTGT CTCCACTGCT GTCACTGTCC ACCTTTGCTG CCCTGTGGCT CACTATCCTA
    GACTTCATGG ACAAGTACAT GCATGCAGGT TCCAGTGATT TGCTGTCAGA AGCTATCCCC
    GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCGG AGATTTTCCA CAGTGCAGAT
    GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG GAGATTACCT GGGAGCGCAT CGACTGCTTC
    TTGCCACACT TGCGTGACGA GCTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCTGCG
    GAACCGCACA GTCAAAAACC TCTGGCCTCT ACTCACCTGA CTTCTGCTGC TGGTGACCCC
    AGGATGCCTG GCCATCCACC TCTCCCAGAG ATACCCTCAG AAATGGGGGT TTGTGACTCA
    GAGAAGCCCG AGAGTACCCG AGCTCCCAGC AGCAGCTCCC CCGGATCGCC CATGGCCTCT
    AGCCCCAGCA AACTGAGTCC TGCCCAAGAG GGACCTCCCC CACTGACCCA GCCCCCACTG
    ATCCTGCAGC CCTTGACCTC CCCACTGCAG GTGGGGGTCC CGCCCATGAC TCTGCCCATC
    ATCCTCAACC CTGCACTCAT TGAGGCCACC TCCCCAGTGC CTCTCCTAGC CACACCCCGC
    CCTACAGACC CCATCCCCAC TTCTGAAGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55180
    Clone ID Related Accession (Same CDS sequence) XM_006526583.2 , XM_006526583.3
    Accession Version XM_006526583.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCAGGA TGTACTTGCC ACTCCAGTGC CAGAGCCATC AGCCTGCTCT GTTCCACACC 
    TTCAGCTTTC AGGGTCTGCG GCTTGATGAA GCTCTCCGAC TCTACCTGGA AGCCTTCCGT
    TTGCCTGGGG AAGCACCCGT TATTCACAGG TTGCTGGAGG TATTCACTGA GCACTGGAGG
    AGTTGTAATG GCTCCCCATT TGCTAATAGT GATGCCTGCT TTGCCCTGGC CTATGCTGTC
    ATCATGCTTA ACACTGACCA ACATAACCAC AATGTTCGAA AGCAGAATGC ACCCATGACC
    CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT GTGAATGGAG GCAAGGACTT TGAGCAAGAC
    ATCCTGGAAG ACATGTACCA TGCCATCAAG AATGAGGAGA TTGTGATGCC TGAGGAGCAG
    ACAGGTCTGG TCCGTGAGAA CTATGTGTGG AGCGTGCTGC TTCACCGAGG TGCTAGCCCT
    GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC AGCTACGATC TTGACCTCTT CACTATGACC
    TGGGGCCCCA CGATCGCTGC TCTCTCTTAT GTCTTCGATA AAAGCCTTGA GGAGACCATC
    ATCCAGAAAG CCATCTCAGG CTTCAGGAAG TGTGCCATGA TCTCCGCCCA CTATGGCCTC
    AGCGATGTGT TTGACAATCT CATCATCTCT CTGTGCAAAT TCACAGCTCT AAGTAGTGAG
    TCTATTGAGA ACCTTCCCAG TGTATTTGGA AGCAACCCTA AAGCCCACAT AGCAGCCAAG
    ACAGTATTCC ATTTGGCCCA TCGTCATGGT GACATCCTTC GGGAGGGCTG GAAGAATATC
    ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC CAACTTTTAC CCAAGGCTAT GGTGGAGGTA
    GAAGATTTTG TGGATCCCAA TGGCAAGATC TCTCTACAGC GGGAGGAGAC ACCATCCAAC
    CGAGGAGAGT CAACAGTTCT TAGCTTTGTA AGCTGGCTGA CATTGAGTGG TCCTGAGCAG
    TCCAGTGTAC GGGGTCCATC TACAGAGAAC CAAGAGGCCA AGAGAGTGGC CTTGGACTGT
    ATCAAGCAAT GTGACCCAGA AAAAATGATC ACAGAAAGCA AGTTCCTCCA GCTGGAATCA
    CTGCAGGAGC TCATGAAGGC TCTGGTCTCA GTGACACCAG ATGAAGAGAC ATATGACGAA
    GAAGATGCTG CTTTCTGTCT AGAGATGCTG CTAAGGATCG TGTTAGAGAA CAGGGACCGT
    GTGGGCTGTG TATGGCAGAC TGTTCGAGAT CATCTATACC ACCTGTGTGT TCAGGCACAA
    GATTTCTGCT TCCTTGTGGA GCGAGCAGTG GTAGGGCTGC TACGCCTAGC CATCCGGCTA
    CTCCGGAGAG AAGAGATTAG TGGCCAGGTC CTGCTCTCTC TGCGCATCTT GTTACTGATG
    AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC CAGGTTGCAT ATGGGCTGCA TGAACTCCTC
    AAGACCAATG CTGCCAACAT CCACTCGGGT GATGACTGGG CCACCCTCTT CACATTGCTG
    GAGTGCATTG GCTCAGGCGT GAAGCCTCCA GATGCTCTTC AAGCCACAGC CAGGGCTGAT
    GCTCCTGATG CGGGAGCACA GTCGGACAGT GAGCTCCCAT CCTACCATCA AAATGATGTC
    AGCCTGGACC GAGGGTACAC TTCCGACTCA GAAGTCTACA CAGACCATGG CAGGCCTGGC
    AAGATACACC GATCGGCCAC AGATGCTGAC ATGGTCAACA GTGGTTGGTT AGTGGTGGGG
    AAGGATGACA TTGATAACTC CAAACCAGGA GCAGGGCTCA GCAGGCCAGG CCCTTCACCC
    CTGGTCAACC AGTACAGCCT TACAGTGGGC CTGGATCTGG GCCCACACGA CACTAAGTCC
    CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC ATTGTCCGAG ATGCTGCTCA CATCACCCCT
    GACAACTTTG AACTCTGCGT CAAGACTCTC CGCATCTTTG TAGAAGCCAG TCTGAATGGT
    GGGTGCAAGT CCCAGGATAA ACGCAGCAAG AGTCATAAGT ATGACAGCAA AGGGAACCGC
    TTCAAGAAAA AACCCAAGGA GGGCTCGATG CTTCGGCGGC CCCGGGGATC CAACCAGCAT
    GCTACTCGGG GCGGGCACAG TGATGAAGAG GAGGATGAAG GTGTGCCTGC CAGCTACCAT
    ACGGTGTCTT TACAGGTCAG TCAGGACTTG CTAGACCTGA TGCATACCCT GCACACACGG
    GCAGCCTCTA TCTACAGCTC ATGGGCAGAG GAGCAACGCC ACCTGGAGGG AGGTGGCCAG
    AAGATTGAAG CTGACTCACG CACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGA
    ATCGCCTGCT TATGCTGTGA CGCCCGGCGC CAGGTACGGA TGCAGGCCCT GACATATCTG
    CAGCGAGCCC TTCTGGTGCA TGACCTACAG AAGCTAGATG CCCTGGAGTG GGAGTCCTGT
    TTTAACAAGG TGCTGTTTCC TCTCCTGACC AAGCTTTTGG AAAATATCAG CCCTGCAGAT
    GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG
    CAGCATCTGT CTCCACTGCT GTCACTGTCC ACCTTTGCTG CCCTGTGGCT CACTATCCTA
    GACTTCATGG ACAAGTACAT GCATGCAGGT TCCAGTGATT TGCTGTCAGA AGCTATCCCC
    GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCGG AGATTTTCCA CAGTGCAGAT
    GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG GAGATTACCT GGGAGCGCAT CGACTGCTTC
    TTGCCACACT TGCGTGACGA GCTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCTGCG
    GAACCGCACA GTCAAAAACC TCTGGCCTCT ACTCACCTGA CTTCTGCTGC TGGTGACCCC
    AGGATGCCTG GCCATCCACC TCTCCCAGAG ATACCCTCAG AAATGGGGGT TTGTGACTCA
    GAGAAGCCCG AGAGTACCCG AGCTCCCAGC AGCAGCTCCC CCGGATCGCC CATGGCCTCT
    AGCCCCAGCA AACTGAGTCC TGCCCAAGAG GGACCTCCCC CACTGACCCA GCCCCCACTG
    ATCCTGCAGC CCTTGACCTC CCCACTGCAG GTGGGGGTCC CGCCCATGAC TCTGCCCATC
    ATCCTCAACC CTGCACTCAT TGAGGCCACC TCCCCAGTGC CTCTCCTAGC CACACCCCGC
    CCTACAGACC CCATCCCCAC TTCTGAAGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526646.1
    CDS16..3351
    Translation

    Target ORF information:

    RefSeq Version XM_006526583.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526583.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCAGGA TGTACTTGCC ACTCCAGTGC CAGAGCCATC AGCCTGCTCT GTTCCACACC 
    TTCAGCTTTC AGGGTCTGCG GCTTGATGAA GCTCTCCGAC TCTACCTGGA AGCCTTCCGT
    TTGCCTGGGG AAGCACCCGT TATTCACAGG TTGCTGGAGG TATTCACTGA GCACTGGAGG
    AGTTGTAATG GCTCCCCATT TGCTAATAGT GATGCCTGCT TTGCCCTGGC CTATGCTGTC
    ATCATGCTTA ACACTGACCA ACATAACCAC AATGTTCGAA AGCAGAATGC ACCCATGACC
    CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT GTGAATGGAG GCAAGGACTT TGAGCAAGAC
    ATCCTGGAAG ACATGTACCA TGCCATCAAG AATGAGGAGA TTGTGATGCC TGAGGAGCAG
    ACAGGTCTGG TCCGTGAGAA CTATGTGTGG AGCGTGCTGC TTCACCGAGG TGCTAGCCCT
    GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC AGCTACGATC TTGACCTCTT CACTATGACC
    TGGGGCCCCA CGATCGCTGC TCTCTCTTAT GTCTTCGATA AAAGCCTTGA GGAGACCATC
    ATCCAGAAAG CCATCTCAGG CTTCAGGAAG TGTGCCATGA TCTCCGCCCA CTATGGCCTC
    AGCGATGTGT TTGACAATCT CATCATCTCT CTGTGCAAAT TCACAGCTCT AAGTAGTGAG
    TCTATTGAGA ACCTTCCCAG TGTATTTGGA AGCAACCCTA AAGCCCACAT AGCAGCCAAG
    ACAGTATTCC ATTTGGCCCA TCGTCATGGT GACATCCTTC GGGAGGGCTG GAAGAATATC
    ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC CAACTTTTAC CCAAGGCTAT GGTGGAGGTA
    GAAGATTTTG TGGATCCCAA TGGCAAGATC TCTCTACAGC GGGAGGAGAC ACCATCCAAC
    CGAGGAGAGT CAACAGTTCT TAGCTTTGTA AGCTGGCTGA CATTGAGTGG TCCTGAGCAG
    TCCAGTGTAC GGGGTCCATC TACAGAGAAC CAAGAGGCCA AGAGAGTGGC CTTGGACTGT
    ATCAAGCAAT GTGACCCAGA AAAAATGATC ACAGAAAGCA AGTTCCTCCA GCTGGAATCA
    CTGCAGGAGC TCATGAAGGC TCTGGTCTCA GTGACACCAG ATGAAGAGAC ATATGACGAA
    GAAGATGCTG CTTTCTGTCT AGAGATGCTG CTAAGGATCG TGTTAGAGAA CAGGGACCGT
    GTGGGCTGTG TATGGCAGAC TGTTCGAGAT CATCTATACC ACCTGTGTGT TCAGGCACAA
    GATTTCTGCT TCCTTGTGGA GCGAGCAGTG GTAGGGCTGC TACGCCTAGC CATCCGGCTA
    CTCCGGAGAG AAGAGATTAG TGGCCAGGTC CTGCTCTCTC TGCGCATCTT GTTACTGATG
    AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC CAGGTTGCAT ATGGGCTGCA TGAACTCCTC
    AAGACCAATG CTGCCAACAT CCACTCGGGT GATGACTGGG CCACCCTCTT CACATTGCTG
    GAGTGCATTG GCTCAGGCGT GAAGCCTCCA GATGCTCTTC AAGCCACAGC CAGGGCTGAT
    GCTCCTGATG CGGGAGCACA GTCGGACAGT GAGCTCCCAT CCTACCATCA AAATGATGTC
    AGCCTGGACC GAGGGTACAC TTCCGACTCA GAAGTCTACA CAGACCATGG CAGGCCTGGC
    AAGATACACC GATCGGCCAC AGATGCTGAC ATGGTCAACA GTGGTTGGTT AGTGGTGGGG
    AAGGATGACA TTGATAACTC CAAACCAGGA GCAGGGCTCA GCAGGCCAGG CCCTTCACCC
    CTGGTCAACC AGTACAGCCT TACAGTGGGC CTGGATCTGG GCCCACACGA CACTAAGTCC
    CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC ATTGTCCGAG ATGCTGCTCA CATCACCCCT
    GACAACTTTG AACTCTGCGT CAAGACTCTC CGCATCTTTG TAGAAGCCAG TCTGAATGGT
    GGGTGCAAGT CCCAGGATAA ACGCAGCAAG AGTCATAAGT ATGACAGCAA AGGGAACCGC
    TTCAAGAAAA AACCCAAGGA GGGCTCGATG CTTCGGCGGC CCCGGGGATC CAACCAGCAT
    GCTACTCGGG GCGGGCACAG TGATGAAGAG GAGGATGAAG GTGTGCCTGC CAGCTACCAT
    ACGGTGTCTT TACAGGTCAG TCAGGACTTG CTAGACCTGA TGCATACCCT GCACACACGG
    GCAGCCTCTA TCTACAGCTC ATGGGCAGAG GAGCAACGCC ACCTGGAGGG AGGTGGCCAG
    AAGATTGAAG CTGACTCACG CACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGA
    ATCGCCTGCT TATGCTGTGA CGCCCGGCGC CAGGTACGGA TGCAGGCCCT GACATATCTG
    CAGCGAGCCC TTCTGGTGCA TGACCTACAG AAGCTAGATG CCCTGGAGTG GGAGTCCTGT
    TTTAACAAGG TGCTGTTTCC TCTCCTGACC AAGCTTTTGG AAAATATCAG CCCTGCAGAT
    GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG
    CAGCATCTGT CTCCACTGCT GTCACTGTCC ACCTTTGCTG CCCTGTGGCT CACTATCCTA
    GACTTCATGG ACAAGTACAT GCATGCAGGT TCCAGTGATT TGCTGTCAGA AGCTATCCCC
    GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCGG AGATTTTCCA CAGTGCAGAT
    GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG GAGATTACCT GGGAGCGCAT CGACTGCTTC
    TTGCCACACT TGCGTGACGA GCTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCTGCG
    GAACCGCACA GTCAAAAACC TCTGGCCTCT ACTCACCTGA CTTCTGCTGC TGGTGACCCC
    AGGATGCCTG GCCATCCACC TCTCCCAGAG ATACCCTCAG AAATGGGGGT TTGTGACTCA
    GAGAAGCCCG AGAGTACCCG AGCTCCCAGC AGCAGCTCCC CCGGATCGCC CATGGCCTCT
    AGCCCCAGCA AACTGAGTCC TGCCCAAGAG GGACCTCCCC CACTGACCCA GCCCCCACTG
    ATCCTGCAGC CCTTGACCTC CCCACTGCAG GTGGGGGTCC CGCCCATGAC TCTGCCCATC
    ATCCTCAACC CTGCACTCAT TGAGGCCACC TCCCCAGTGC CTCTCCTAGC CACACCCCGC
    CCTACAGACC CCATCCCCAC TTCTGAAGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07918
    Clone ID Related Accession (Same CDS sequence) NM_178930.3
    Accession Version NM_178930.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-27
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076569.1. On or before Sep 17, 2004 this sequence version replaced XM_129295.6, NM_178930.2. ##Evidence-Data-START## Transcript exon combination :: BC076569.1, BC082336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCATACCCT GCACACACGG GCAGCCTCTA TCTACAGCTC ATGGGCAGAG
    GAGCAACGCC ACCTGGAGGG AGGTGGCCAG AAGATTGAAG CTGACTCACG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ATTACAAGGA ATCGCCTGCT TATGCTGTGA CGCCCGGCGC
    CAGGTACGGA TGCAGGCCCT GACATATCTG CAGCGAGCCC TTCTGGTGCA TGACCTACAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTCCTGACC
    AAGCTTTTGG AAAATATCAG CCCTGCAGAT GTGGGCGGGA TGGAGGAGAC CCGGATGCGG
    GCTTCCACAC TGCTCTCAAA GGTCTTCCTG CAGCATCTGT CTCCACTGCT GTCACTGTCC
    ACCTTTGCTG CCCTGTGGCT CACTATCCTA GACTTCATGG ACAAGTACAT GCATGCAGGT
    TCCAGTGATT TGCTGTCAGA AGCTATCCCC GAGTCTCTGA AGAACATGCT GCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG
    GAGATTACCT GGGAGCGCAT CGACTGCTTC TTGCCACACT TGCGTGACGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCTGCG GAACCGCACA GTCAAAAACC TCTGGCCTCT
    ACTCACCTGA CTTCTGCTGC TGGTGACCCC AGGATGCCTG GCCATCCACC TCTCCCAGAG
    ATACCCTCAG AAATGGGGGT TTGTGACTCA GAGAAGCCCG AGAGTACCCG AGCTCCCAGC
    AGCAGCTCCC CCGGATCGCC CATGGCCTCT AGCCCCAGCA AACTGAGTCC TGCCCAAGAG
    GGACCTCCCC CACTGACCCA GCCCCCACTG ATCCTGCAGC CCTTGACCTC CCCACTGCAG
    GTGGGGGTCC CGCCCATGAC TCTGCCCATC ATCCTCAACC CTGCACTCAT TGAGGCCACC
    TCCCCAGTGC CTCTCCTAGC CACACCCCGC CCTACAGACC CCATCCCCAC TTCTGAAGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_849261.2
    CDS246..5831
    Translation

    Target ORF information:

    RefSeq Version NM_178930.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_178930.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCATACCCT GCACACACGG GCAGCCTCTA TCTACAGCTC ATGGGCAGAG
    GAGCAACGCC ACCTGGAGGG AGGTGGCCAG AAGATTGAAG CTGACTCACG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ATTACAAGGA ATCGCCTGCT TATGCTGTGA CGCCCGGCGC
    CAGGTACGGA TGCAGGCCCT GACATATCTG CAGCGAGCCC TTCTGGTGCA TGACCTACAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTCCTGACC
    AAGCTTTTGG AAAATATCAG CCCTGCAGAT GTGGGCGGGA TGGAGGAGAC CCGGATGCGG
    GCTTCCACAC TGCTCTCAAA GGTCTTCCTG CAGCATCTGT CTCCACTGCT GTCACTGTCC
    ACCTTTGCTG CCCTGTGGCT CACTATCCTA GACTTCATGG ACAAGTACAT GCATGCAGGT
    TCCAGTGATT TGCTGTCAGA AGCTATCCCC GAGTCTCTGA AGAACATGCT GCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCAAGAGGAG GAGGCCCCTC TGCTCTCTGG
    GAGATTACCT GGGAGCGCAT CGACTGCTTC TTGCCACACT TGCGTGACGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCTGCG GAACCGCACA GTCAAAAACC TCTGGCCTCT
    ACTCACCTGA CTTCTGCTGC TGGTGACCCC AGGATGCCTG GCCATCCACC TCTCCCAGAG
    ATACCCTCAG AAATGGGGGT TTGTGACTCA GAGAAGCCCG AGAGTACCCG AGCTCCCAGC
    AGCAGCTCCC CCGGATCGCC CATGGCCTCT AGCCCCAGCA AACTGAGTCC TGCCCAAGAG
    GGACCTCCCC CACTGACCCA GCCCCCACTG ATCCTGCAGC CCTTGACCTC CCCACTGCAG
    GTGGGGGTCC CGCCCATGAC TCTGCCCATC ATCCTCAACC CTGCACTCAT TGAGGCCACC
    TCCCCAGTGC CTCTCCTAGC CACACCCCGC CCTACAGACC CCATCCCCAC TTCTGAAGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55177
    Clone ID Related Accession (Same CDS sequence) XM_006526580.2 , XM_006526580.3
    Accession Version XM_006526580.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGCTAGACC TGATGCATAC CCTGCACACA CGGGCAGCCT CTATCTACAG CTCATGGGCA
    GAGGAGCAAC GCCACCTGGA GGGAGGTGGC CAGAAGATTG AAGCTGACTC ACGCACCCTC
    TGGGCCCACT GCTGGTGCCC TTTATTACAA GGAATCGCCT GCTTATGCTG TGACGCCCGG
    CGCCAGGTAC GGATGCAGGC CCTGACATAT CTGCAGCGAG CCCTTCTGGT GCATGACCTA
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTCCTG
    ACCAAGCTTT TGGAAAATAT CAGCCCTGCA GATGTGGGCG GGATGGAGGA GACCCGGATG
    CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCATC TGTCTCCACT GCTGTCACTG
    TCCACCTTTG CTGCCCTGTG GCTCACTATC CTAGACTTCA TGGACAAGTA CATGCATGCA
    GGTTCCAGTG ATTTGCTGTC AGAAGCTATC CCCGAGTCTC TGAAGAACAT GCTGCTGGTG
    ATGGACACAG CGGAGATTTT CCACAGTGCA GATGCAAGAG GAGGAGGCCC CTCTGCTCTC
    TGGGAGATTA CCTGGGAGCG CATCGACTGC TTCTTGCCAC ACTTGCGTGA CGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCT GCGGAACCGC ACAGTCAAAA ACCTCTGGCC
    TCTACTCACC TGACTTCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCTCCCA
    GAGATACCCT CAGAAATGGG GGTTTGTGAC TCAGAGAAGC CCGAGAGTAC CCGAGCTCCC
    AGCAGCAGCT CCCCCGGATC GCCCATGGCC TCTAGCCCCA GCAAACTGAG TCCTGCCCAA
    GAGGGACCTC CCCCACTGAC CCAGCCCCCA CTGATCCTGC AGCCCTTGAC CTCCCCACTG
    CAGGTGGGGG TCCCGCCCAT GACTCTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC
    ACCTCCCCAG TGCCTCTCCT AGCCACACCC CGCCCTACAG ACCCCATCCC CACTTCTGAA
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526643.1
    CDS294..5882
    Translation

    Target ORF information:

    RefSeq Version XM_006526580.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526580.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGCTAGACC TGATGCATAC CCTGCACACA CGGGCAGCCT CTATCTACAG CTCATGGGCA
    GAGGAGCAAC GCCACCTGGA GGGAGGTGGC CAGAAGATTG AAGCTGACTC ACGCACCCTC
    TGGGCCCACT GCTGGTGCCC TTTATTACAA GGAATCGCCT GCTTATGCTG TGACGCCCGG
    CGCCAGGTAC GGATGCAGGC CCTGACATAT CTGCAGCGAG CCCTTCTGGT GCATGACCTA
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTCCTG
    ACCAAGCTTT TGGAAAATAT CAGCCCTGCA GATGTGGGCG GGATGGAGGA GACCCGGATG
    CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCATC TGTCTCCACT GCTGTCACTG
    TCCACCTTTG CTGCCCTGTG GCTCACTATC CTAGACTTCA TGGACAAGTA CATGCATGCA
    GGTTCCAGTG ATTTGCTGTC AGAAGCTATC CCCGAGTCTC TGAAGAACAT GCTGCTGGTG
    ATGGACACAG CGGAGATTTT CCACAGTGCA GATGCAAGAG GAGGAGGCCC CTCTGCTCTC
    TGGGAGATTA CCTGGGAGCG CATCGACTGC TTCTTGCCAC ACTTGCGTGA CGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCT GCGGAACCGC ACAGTCAAAA ACCTCTGGCC
    TCTACTCACC TGACTTCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCTCCCA
    GAGATACCCT CAGAAATGGG GGTTTGTGAC TCAGAGAAGC CCGAGAGTAC CCGAGCTCCC
    AGCAGCAGCT CCCCCGGATC GCCCATGGCC TCTAGCCCCA GCAAACTGAG TCCTGCCCAA
    GAGGGACCTC CCCCACTGAC CCAGCCCCCA CTGATCCTGC AGCCCTTGAC CTCCCCACTG
    CAGGTGGGGG TCCCGCCCAT GACTCTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC
    ACCTCCCCAG TGCCTCTCCT AGCCACACCC CGCCCTACAG ACCCCATCCC CACTTCTGAA
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55178
    Clone ID Related Accession (Same CDS sequence) XM_006526581.2 , XM_006526581.3
    Accession Version XM_006526581.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG
    ATGCATACCC TGCACACACG GGCAGCCTCT ATCTACAGCT CATGGGCAGA GGAGCAACGC
    CACCTGGAGG GAGGTGGCCA GAAGATTGAA GCTGACTCAC GCACCCTCTG GGCCCACTGC
    TGGTGCCCTT TATTACAAGG AATCGCCTGC TTATGCTGTG ACGCCCGGCG CCAGGTACGG
    ATGCAGGCCC TGACATATCT GCAGCGAGCC CTTCTGGTGC ATGACCTACA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTCCTGAC CAAGCTTTTG
    GAAAATATCA GCCCTGCAGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA
    CTGCTCTCAA AGGTCTTCCT GCAGCATCTG TCTCCACTGC TGTCACTGTC CACCTTTGCT
    GCCCTGTGGC TCACTATCCT AGACTTCATG GACAAGTACA TGCATGCAGG TTCCAGTGAT
    TTGCTGTCAG AAGCTATCCC CGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCG
    GAGATTTTCC ACAGTGCAGA TGCAAGAGGA GGAGGCCCCT CTGCTCTCTG GGAGATTACC
    TGGGAGCGCA TCGACTGCTT CTTGCCACAC TTGCGTGACG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCTGC GGAACCGCAC AGTCAAAAAC CTCTGGCCTC TACTCACCTG
    ACTTCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCTCCCAGA GATACCCTCA
    GAAATGGGGG TTTGTGACTC AGAGAAGCCC GAGAGTACCC GAGCTCCCAG CAGCAGCTCC
    CCCGGATCGC CCATGGCCTC TAGCCCCAGC AAACTGAGTC CTGCCCAAGA GGGACCTCCC
    CCACTGACCC AGCCCCCACT GATCCTGCAG CCCTTGACCT CCCCACTGCA GGTGGGGGTC
    CCGCCCATGA CTCTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCCCCAGTG
    CCTCTCCTAG CCACACCCCG CCCTACAGAC CCCATCCCCA CTTCTGAAGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526644.1
    CDS294..5870
    Translation

    Target ORF information:

    RefSeq Version XM_006526581.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526581.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG
    ATGCATACCC TGCACACACG GGCAGCCTCT ATCTACAGCT CATGGGCAGA GGAGCAACGC
    CACCTGGAGG GAGGTGGCCA GAAGATTGAA GCTGACTCAC GCACCCTCTG GGCCCACTGC
    TGGTGCCCTT TATTACAAGG AATCGCCTGC TTATGCTGTG ACGCCCGGCG CCAGGTACGG
    ATGCAGGCCC TGACATATCT GCAGCGAGCC CTTCTGGTGC ATGACCTACA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTCCTGAC CAAGCTTTTG
    GAAAATATCA GCCCTGCAGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA
    CTGCTCTCAA AGGTCTTCCT GCAGCATCTG TCTCCACTGC TGTCACTGTC CACCTTTGCT
    GCCCTGTGGC TCACTATCCT AGACTTCATG GACAAGTACA TGCATGCAGG TTCCAGTGAT
    TTGCTGTCAG AAGCTATCCC CGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCG
    GAGATTTTCC ACAGTGCAGA TGCAAGAGGA GGAGGCCCCT CTGCTCTCTG GGAGATTACC
    TGGGAGCGCA TCGACTGCTT CTTGCCACAC TTGCGTGACG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCTGC GGAACCGCAC AGTCAAAAAC CTCTGGCCTC TACTCACCTG
    ACTTCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCTCCCAGA GATACCCTCA
    GAAATGGGGG TTTGTGACTC AGAGAAGCCC GAGAGTACCC GAGCTCCCAG CAGCAGCTCC
    CCCGGATCGC CCATGGCCTC TAGCCCCAGC AAACTGAGTC CTGCCCAAGA GGGACCTCCC
    CCACTGACCC AGCCCCCACT GATCCTGCAG CCCTTGACCT CCCCACTGCA GGTGGGGGTC
    CCGCCCATGA CTCTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCCCCAGTG
    CCTCTCCTAG CCACACCCCG CCCTACAGAC CCCATCCCCA CTTCTGAAGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55179
    Clone ID Related Accession (Same CDS sequence) XM_006526582.4 , XM_006526582.3
    Accession Version XM_006526582.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CATACCCTGC ACACACGGGC AGCCTCTATC TACAGCTCAT GGGCAGAGGA GCAACGCCAC
    CTGGAGGGAG GTGGCCAGAA GATTGAAGCT GACTCACGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAT TACAAGGAAT CGCCTGCTTA TGCTGTGACG CCCGGCGCCA GGTACGGATG
    CAGGCCCTGA CATATCTGCA GCGAGCCCTT CTGGTGCATG ACCTACAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TCCTGACCAA GCTTTTGGAA
    AATATCAGCC CTGCAGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG
    CTCTCAAAGG TCTTCCTGCA GCATCTGTCT CCACTGCTGT CACTGTCCAC CTTTGCTGCC
    CTGTGGCTCA CTATCCTAGA CTTCATGGAC AAGTACATGC ATGCAGGTTC CAGTGATTTG
    CTGTCAGAAG CTATCCCCGA GTCTCTGAAG AACATGCTGC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC AAGAGGAGGA GGCCCCTCTG CTCTCTGGGA GATTACCTGG
    GAGCGCATCG ACTGCTTCTT GCCACACTTG CGTGACGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCTGCGGA ACCGCACAGT CAAAAACCTC TGGCCTCTAC TCACCTGACT
    TCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC TCCCAGAGAT ACCCTCAGAA
    ATGGGGGTTT GTGACTCAGA GAAGCCCGAG AGTACCCGAG CTCCCAGCAG CAGCTCCCCC
    GGATCGCCCA TGGCCTCTAG CCCCAGCAAA CTGAGTCCTG CCCAAGAGGG ACCTCCCCCA
    CTGACCCAGC CCCCACTGAT CCTGCAGCCC TTGACCTCCC CACTGCAGGT GGGGGTCCCG
    CCCATGACTC TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC CCCAGTGCCT
    CTCCTAGCCA CACCCCGCCC TACAGACCCC ATCCCCACTT CTGAAGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526645.1
    CDS295..5868
    Translation

    Target ORF information:

    RefSeq Version XM_006526582.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526582.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CATACCCTGC ACACACGGGC AGCCTCTATC TACAGCTCAT GGGCAGAGGA GCAACGCCAC
    CTGGAGGGAG GTGGCCAGAA GATTGAAGCT GACTCACGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAT TACAAGGAAT CGCCTGCTTA TGCTGTGACG CCCGGCGCCA GGTACGGATG
    CAGGCCCTGA CATATCTGCA GCGAGCCCTT CTGGTGCATG ACCTACAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TCCTGACCAA GCTTTTGGAA
    AATATCAGCC CTGCAGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG
    CTCTCAAAGG TCTTCCTGCA GCATCTGTCT CCACTGCTGT CACTGTCCAC CTTTGCTGCC
    CTGTGGCTCA CTATCCTAGA CTTCATGGAC AAGTACATGC ATGCAGGTTC CAGTGATTTG
    CTGTCAGAAG CTATCCCCGA GTCTCTGAAG AACATGCTGC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC AAGAGGAGGA GGCCCCTCTG CTCTCTGGGA GATTACCTGG
    GAGCGCATCG ACTGCTTCTT GCCACACTTG CGTGACGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCTGCGGA ACCGCACAGT CAAAAACCTC TGGCCTCTAC TCACCTGACT
    TCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC TCCCAGAGAT ACCCTCAGAA
    ATGGGGGTTT GTGACTCAGA GAAGCCCGAG AGTACCCGAG CTCCCAGCAG CAGCTCCCCC
    GGATCGCCCA TGGCCTCTAG CCCCAGCAAA CTGAGTCCTG CCCAAGAGGG ACCTCCCCCA
    CTGACCCAGC CCCCACTGAT CCTGCAGCCC TTGACCTCCC CACTGCAGGT GGGGGTCCCG
    CCCATGACTC TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC CCCAGTGCCT
    CTCCTAGCCA CACCCCGCCC TACAGACCCC ATCCCCACTT CTGAAGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55177
    Clone ID Related Accession (Same CDS sequence) XM_006526580.2 , XM_006526580.3
    Accession Version XM_006526580.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGCTAGACC TGATGCATAC CCTGCACACA CGGGCAGCCT CTATCTACAG CTCATGGGCA
    GAGGAGCAAC GCCACCTGGA GGGAGGTGGC CAGAAGATTG AAGCTGACTC ACGCACCCTC
    TGGGCCCACT GCTGGTGCCC TTTATTACAA GGAATCGCCT GCTTATGCTG TGACGCCCGG
    CGCCAGGTAC GGATGCAGGC CCTGACATAT CTGCAGCGAG CCCTTCTGGT GCATGACCTA
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTCCTG
    ACCAAGCTTT TGGAAAATAT CAGCCCTGCA GATGTGGGCG GGATGGAGGA GACCCGGATG
    CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCATC TGTCTCCACT GCTGTCACTG
    TCCACCTTTG CTGCCCTGTG GCTCACTATC CTAGACTTCA TGGACAAGTA CATGCATGCA
    GGTTCCAGTG ATTTGCTGTC AGAAGCTATC CCCGAGTCTC TGAAGAACAT GCTGCTGGTG
    ATGGACACAG CGGAGATTTT CCACAGTGCA GATGCAAGAG GAGGAGGCCC CTCTGCTCTC
    TGGGAGATTA CCTGGGAGCG CATCGACTGC TTCTTGCCAC ACTTGCGTGA CGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCT GCGGAACCGC ACAGTCAAAA ACCTCTGGCC
    TCTACTCACC TGACTTCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCTCCCA
    GAGATACCCT CAGAAATGGG GGTTTGTGAC TCAGAGAAGC CCGAGAGTAC CCGAGCTCCC
    AGCAGCAGCT CCCCCGGATC GCCCATGGCC TCTAGCCCCA GCAAACTGAG TCCTGCCCAA
    GAGGGACCTC CCCCACTGAC CCAGCCCCCA CTGATCCTGC AGCCCTTGAC CTCCCCACTG
    CAGGTGGGGG TCCCGCCCAT GACTCTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC
    ACCTCCCCAG TGCCTCTCCT AGCCACACCC CGCCCTACAG ACCCCATCCC CACTTCTGAA
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526643.1
    CDS295..5883
    Translation

    Target ORF information:

    RefSeq Version XM_006526580.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGCTAGACC TGATGCATAC CCTGCACACA CGGGCAGCCT CTATCTACAG CTCATGGGCA
    GAGGAGCAAC GCCACCTGGA GGGAGGTGGC CAGAAGATTG AAGCTGACTC ACGCACCCTC
    TGGGCCCACT GCTGGTGCCC TTTATTACAA GGAATCGCCT GCTTATGCTG TGACGCCCGG
    CGCCAGGTAC GGATGCAGGC CCTGACATAT CTGCAGCGAG CCCTTCTGGT GCATGACCTA
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTCCTG
    ACCAAGCTTT TGGAAAATAT CAGCCCTGCA GATGTGGGCG GGATGGAGGA GACCCGGATG
    CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCATC TGTCTCCACT GCTGTCACTG
    TCCACCTTTG CTGCCCTGTG GCTCACTATC CTAGACTTCA TGGACAAGTA CATGCATGCA
    GGTTCCAGTG ATTTGCTGTC AGAAGCTATC CCCGAGTCTC TGAAGAACAT GCTGCTGGTG
    ATGGACACAG CGGAGATTTT CCACAGTGCA GATGCAAGAG GAGGAGGCCC CTCTGCTCTC
    TGGGAGATTA CCTGGGAGCG CATCGACTGC TTCTTGCCAC ACTTGCGTGA CGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCT GCGGAACCGC ACAGTCAAAA ACCTCTGGCC
    TCTACTCACC TGACTTCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCTCCCA
    GAGATACCCT CAGAAATGGG GGTTTGTGAC TCAGAGAAGC CCGAGAGTAC CCGAGCTCCC
    AGCAGCAGCT CCCCCGGATC GCCCATGGCC TCTAGCCCCA GCAAACTGAG TCCTGCCCAA
    GAGGGACCTC CCCCACTGAC CCAGCCCCCA CTGATCCTGC AGCCCTTGAC CTCCCCACTG
    CAGGTGGGGG TCCCGCCCAT GACTCTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC
    ACCTCCCCAG TGCCTCTCCT AGCCACACCC CGCCCTACAG ACCCCATCCC CACTTCTGAA
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55178
    Clone ID Related Accession (Same CDS sequence) XM_006526581.2 , XM_006526581.3
    Accession Version XM_006526581.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG
    ATGCATACCC TGCACACACG GGCAGCCTCT ATCTACAGCT CATGGGCAGA GGAGCAACGC
    CACCTGGAGG GAGGTGGCCA GAAGATTGAA GCTGACTCAC GCACCCTCTG GGCCCACTGC
    TGGTGCCCTT TATTACAAGG AATCGCCTGC TTATGCTGTG ACGCCCGGCG CCAGGTACGG
    ATGCAGGCCC TGACATATCT GCAGCGAGCC CTTCTGGTGC ATGACCTACA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTCCTGAC CAAGCTTTTG
    GAAAATATCA GCCCTGCAGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA
    CTGCTCTCAA AGGTCTTCCT GCAGCATCTG TCTCCACTGC TGTCACTGTC CACCTTTGCT
    GCCCTGTGGC TCACTATCCT AGACTTCATG GACAAGTACA TGCATGCAGG TTCCAGTGAT
    TTGCTGTCAG AAGCTATCCC CGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCG
    GAGATTTTCC ACAGTGCAGA TGCAAGAGGA GGAGGCCCCT CTGCTCTCTG GGAGATTACC
    TGGGAGCGCA TCGACTGCTT CTTGCCACAC TTGCGTGACG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCTGC GGAACCGCAC AGTCAAAAAC CTCTGGCCTC TACTCACCTG
    ACTTCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCTCCCAGA GATACCCTCA
    GAAATGGGGG TTTGTGACTC AGAGAAGCCC GAGAGTACCC GAGCTCCCAG CAGCAGCTCC
    CCCGGATCGC CCATGGCCTC TAGCCCCAGC AAACTGAGTC CTGCCCAAGA GGGACCTCCC
    CCACTGACCC AGCCCCCACT GATCCTGCAG CCCTTGACCT CCCCACTGCA GGTGGGGGTC
    CCGCCCATGA CTCTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCCCCAGTG
    CCTCTCCTAG CCACACCCCG CCCTACAGAC CCCATCCCCA CTTCTGAAGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526644.1
    CDS295..5871
    Translation

    Target ORF information:

    RefSeq Version XM_006526581.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GCAGGAAGGG
    GCCCATGTAG AGAAGGCCCA GTCAGCATCG GTGGAGTCCA TCCCTGAAGT GTTAGAGGAA
    TGCACATCCC CTGCTGACCA CTCTGACTCG GCCTCTGTCC ATGATATGGA CTACGTCAAT
    CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT
    GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACACGAC
    CGCCATAACT CTGAGGTGAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAG
    TCAGCGCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TTAAGGATGA GATGTGCCGA
    CACTTATTCC AGCTACTCAG CGTAGAGCGC CTGAACCTTT ATGCTGCTTC CCTACGAGTG
    TGCTTCTTAC TCTTCGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTAGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCGCTGG AGGCCGTTGT TCAGCTCTGG CGTATCCCCA GCTTCGTCAC TGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTATTGCTCC AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTTCCTGT GTCTGGTCAG CTTTATACAA CACACCTGCT GTCTCTTGAT
    GCCCTTTTGA CAGTTATTGA CAGCACTGAG TCTCATTGTC AGGCCAAAGT CCTCAACACC
    CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAAC
    CCAGAAACTA ACAAGTCTGA GAGAGCAACC AGTGATGGGA AATCCACAGG TGTGGCCTTA
    GATGCCCGAG GCCTTCATTT TCCAAGTGGA GGATGGTTGT CAACAGAACA TGGAAAGCCA
    GGGTGCAGAG ATCTGGAGGA AGCTGGTGAC TCTGGGGCTG ACAAAAAGTT CACCAGGAAG
    CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAA
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACGGAGGT AGCCCAATGG
    CTCCGAGAAA ACCCTCGGTT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTTGAT
    GAAGCTCTCC GACTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC CGTTATTCAC
    AGGTTGCTGG AGGTATTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT
    AGTGATGCCT GCTTTGCCCT GGCCTATGCT GTCATCATGC TTAACACTGA CCAACATAAC
    CACAATGTTC GAAAGCAGAA TGCACCCATG ACCCTGGAGG AATTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC
    AAGAATGAGG AGATTGTGAT GCCTGAGGAG CAGACAGGTC TGGTCCGTGA GAACTATGTG
    TGGAGCGTGC TGCTTCACCG AGGTGCTAGC CCTGAGGGCG TGTTCCTTCG AGTGCCTCCT
    GGCAGCTACG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACGATCGC TGCTCTCTCT
    TATGTCTTCG ATAAAAGCCT TGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTTCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA CATAGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGTTGCA GCTTTTCCGA
    GCCCAACTTT TACCCAAGGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAGGA GACACCATCC AACCGAGGAG AGTCAACAGT TCTTAGCTTT
    GTAAGCTGGC TGACATTGAG TGGTCCTGAG CAGTCCAGTG TACGGGGTCC ATCTACAGAG
    AACCAAGAGG CCAAGAGAGT GGCCTTGGAC TGTATCAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAC GAAGAAGATG CTGCTTTCTG TCTAGAGATG
    CTGCTAAGGA TCGTGTTAGA GAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA
    GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA
    GTGGTAGGGC TGCTACGCCT AGCCATCCGG CTACTCCGGA GAGAAGAGAT TAGTGGCCAG
    GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC
    CACCAGGTTG CATATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG
    GGTGATGACT GGGCCACCCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT
    CCAGATGCTC TTCAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCGGGAGC ACAGTCGGAC
    AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTGG ACCGAGGGTA CACTTCCGAC
    TCAGAAGTCT ACACAGACCA TGGCAGGCCT GGCAAGATAC ACCGATCGGC CACAGATGCT
    GACATGGTCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA
    GGAGCAGGGC TCAGCAGGCC AGGCCCTTCA CCCCTGGTCA ACCAGTACAG CCTTACAGTG
    GGCCTGGATC TGGGCCCACA CGACACTAAG TCCCTGCTTA AGTGTGTGGA GTCACTGTCC
    TTCATTGTCC GAGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACT
    CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGCAGC
    AAGAGTCATA AGTATGACAG CAAAGGGAAC CGCTTCAAGA AAAAACCCAA GGAGGGCTCG
    ATGCTTCGGC GGCCCCGGGG ATCCAACCAG CATGCTACTC GGGGCGGGCA CAGTGATGAA
    GAGGAGGATG AAGGTGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG
    ATGCATACCC TGCACACACG GGCAGCCTCT ATCTACAGCT CATGGGCAGA GGAGCAACGC
    CACCTGGAGG GAGGTGGCCA GAAGATTGAA GCTGACTCAC GCACCCTCTG GGCCCACTGC
    TGGTGCCCTT TATTACAAGG AATCGCCTGC TTATGCTGTG ACGCCCGGCG CCAGGTACGG
    ATGCAGGCCC TGACATATCT GCAGCGAGCC CTTCTGGTGC ATGACCTACA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTCCTGAC CAAGCTTTTG
    GAAAATATCA GCCCTGCAGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA
    CTGCTCTCAA AGGTCTTCCT GCAGCATCTG TCTCCACTGC TGTCACTGTC CACCTTTGCT
    GCCCTGTGGC TCACTATCCT AGACTTCATG GACAAGTACA TGCATGCAGG TTCCAGTGAT
    TTGCTGTCAG AAGCTATCCC CGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCG
    GAGATTTTCC ACAGTGCAGA TGCAAGAGGA GGAGGCCCCT CTGCTCTCTG GGAGATTACC
    TGGGAGCGCA TCGACTGCTT CTTGCCACAC TTGCGTGACG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCTGC GGAACCGCAC AGTCAAAAAC CTCTGGCCTC TACTCACCTG
    ACTTCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCTCCCAGA GATACCCTCA
    GAAATGGGGG TTTGTGACTC AGAGAAGCCC GAGAGTACCC GAGCTCCCAG CAGCAGCTCC
    CCCGGATCGC CCATGGCCTC TAGCCCCAGC AAACTGAGTC CTGCCCAAGA GGGACCTCCC
    CCACTGACCC AGCCCCCACT GATCCTGCAG CCCTTGACCT CCCCACTGCA GGTGGGGGTC
    CCGCCCATGA CTCTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCCCCAGTG
    CCTCTCCTAG CCACACCCCG CCCTACAGAC CCCATCCCCA CTTCTGAAGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55179
    Clone ID Related Accession (Same CDS sequence) XM_006526582.4 , XM_006526582.3
    Accession Version XM_006526582.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CATACCCTGC ACACACGGGC AGCCTCTATC TACAGCTCAT GGGCAGAGGA GCAACGCCAC
    CTGGAGGGAG GTGGCCAGAA GATTGAAGCT GACTCACGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAT TACAAGGAAT CGCCTGCTTA TGCTGTGACG CCCGGCGCCA GGTACGGATG
    CAGGCCCTGA CATATCTGCA GCGAGCCCTT CTGGTGCATG ACCTACAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TCCTGACCAA GCTTTTGGAA
    AATATCAGCC CTGCAGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG
    CTCTCAAAGG TCTTCCTGCA GCATCTGTCT CCACTGCTGT CACTGTCCAC CTTTGCTGCC
    CTGTGGCTCA CTATCCTAGA CTTCATGGAC AAGTACATGC ATGCAGGTTC CAGTGATTTG
    CTGTCAGAAG CTATCCCCGA GTCTCTGAAG AACATGCTGC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC AAGAGGAGGA GGCCCCTCTG CTCTCTGGGA GATTACCTGG
    GAGCGCATCG ACTGCTTCTT GCCACACTTG CGTGACGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCTGCGGA ACCGCACAGT CAAAAACCTC TGGCCTCTAC TCACCTGACT
    TCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC TCCCAGAGAT ACCCTCAGAA
    ATGGGGGTTT GTGACTCAGA GAAGCCCGAG AGTACCCGAG CTCCCAGCAG CAGCTCCCCC
    GGATCGCCCA TGGCCTCTAG CCCCAGCAAA CTGAGTCCTG CCCAAGAGGG ACCTCCCCCA
    CTGACCCAGC CCCCACTGAT CCTGCAGCCC TTGACCTCCC CACTGCAGGT GGGGGTCCCG
    CCCATGACTC TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC CCCAGTGCCT
    CTCCTAGCCA CACCCCGCCC TACAGACCCC ATCCCCACTT CTGAAGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526645.1
    CDS274..5847
    Translation

    Target ORF information:

    RefSeq Version XM_006526582.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus golgi-specific brefeldin A-resistance factor 1 (Gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526582.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAACGGGA TCCCCTTCTG
    CACAGTTTCA GTAACCTGAA AGAGGTCTTA AACAGTGTGA CAGAACTCTC AGAGATTGAG
    CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT
    ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTACGCACT CATAGATCCA
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC
    CGAACTCTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT
    ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGACTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTGGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAGCTATGT GGGAACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGACT CGTCCAAGTG GAAGAAACAG AAAAGATCTC CTCGACCTCC ACGTCATACA
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCACATT ACCCTCTAAC
    CTTACTGGTG GTGTGCCCTT CATTGATGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCGA CAGTAGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGACT
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC
    AGTGAGCCTG GGAGCAGTGA GATGGGCGCT TCTGATCAGC TTGACCCTCA GGAAGGGGCC
    CATGTAGAGA AGGCCCAGTC AGCATCGGTG GAGTCCATCC CTGAAGTGTT AGAGGAATGC
    ACATCCCCTG CTGACCACTC TGACTCGGCC TCTGTCCATG ATATGGACTA CGTCAATCCT
    CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT
    CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACACGACCGC
    CATAACTCTG AGGTGATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAGTCA
    GCGCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATTA AGGATGAGAT GTGCCGACAC
    TTATTCCAGC TACTCAGCGT AGAGCGCCTG AACCTTTATG CTGCTTCCCT ACGAGTGTGC
    TTCTTACTCT TCGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTAGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCGCTGGAGG CCGTTGTTCA GCTCTGGCGT ATCCCCAGCT TCGTCACTGA GCTCTACATC
    AACTATGATT GTGACTACTA TTGCTCCAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TTCCTGTGTC TGGTCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC
    CTTTTGACAG TTATTGACAG CACTGAGTCT CATTGTCAGG CCAAAGTCCT CAACACCCTC
    AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAACCCA
    GAAACTAACA AGTCTGAGAG AGCAACCAGT GATGGGAAAT CCACAGGTGT GGCCTTAGAT
    GCCCGAGGCC TTCATTTTCC AAGTGGAGGA TGGTTGTCAA CAGAACATGG AAAGCCAGGG
    TGCAGAGATC TGGAGGAAGC TGGTGACTCT GGGGCTGACA AAAAGTTCAC CAGGAAGCCA
    CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA
    AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAATTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CGGAGGTAGC CCAATGGCTC
    CGAGAAAACC CTCGGTTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTTGATGAA
    GCTCTCCGAC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCCGT TATTCACAGG
    TTGCTGGAGG TATTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGT
    GATGCCTGCT TTGCCCTGGC CTATGCTGTC ATCATGCTTA ACACTGACCA ACATAACCAC
    AATGTTCGAA AGCAGAATGC ACCCATGACC CTGGAGGAAT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG
    AATGAGGAGA TTGTGATGCC TGAGGAGCAG ACAGGTCTGG TCCGTGAGAA CTATGTGTGG
    AGCGTGCTGC TTCACCGAGG TGCTAGCCCT GAGGGCGTGT TCCTTCGAGT GCCTCCTGGC
    AGCTACGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CGATCGCTGC TCTCTCTTAT
    GTCTTCGATA AAAGCCTTGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTTCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCACAT AGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGTTGCAGCT TTTCCGAGCC
    CAACTTTTAC CCAAGGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAGGAGAC ACCATCCAAC CGAGGAGAGT CAACAGTTCT TAGCTTTGTA
    AGCTGGCTGA CATTGAGTGG TCCTGAGCAG TCCAGTGTAC GGGGTCCATC TACAGAGAAC
    CAAGAGGCCA AGAGAGTGGC CTTGGACTGT ATCAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAATCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGACGAA GAAGATGCTG CTTTCTGTCT AGAGATGCTG
    CTAAGGATCG TGTTAGAGAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT
    CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG
    GTAGGGCTGC TACGCCTAGC CATCCGGCTA CTCCGGAGAG AAGAGATTAG TGGCCAGGTC
    CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC
    CAGGTTGCAT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT
    GATGACTGGG CCACCCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA
    GATGCTCTTC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CGGGAGCACA GTCGGACAGT
    GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTGGACC GAGGGTACAC TTCCGACTCA
    GAAGTCTACA CAGACCATGG CAGGCCTGGC AAGATACACC GATCGGCCAC AGATGCTGAC
    ATGGTCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGGA
    GCAGGGCTCA GCAGGCCAGG CCCTTCACCC CTGGTCAACC AGTACAGCCT TACAGTGGGC
    CTGGATCTGG GCCCACACGA CACTAAGTCC CTGCTTAAGT GTGTGGAGTC ACTGTCCTTC
    ATTGTCCGAG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACTCTC
    CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGCAGCAAG
    AGTCATAAGT ATGACAGCAA AGGGAACCGC TTCAAGAAAA AACCCAAGGA GGGCTCGATG
    CTTCGGCGGC CCCGGGGATC CAACCAGCAT GCTACTCGGG GCGGGCACAG TGATGAAGAG
    GAGGATGAAG GTGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CATACCCTGC ACACACGGGC AGCCTCTATC TACAGCTCAT GGGCAGAGGA GCAACGCCAC
    CTGGAGGGAG GTGGCCAGAA GATTGAAGCT GACTCACGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAT TACAAGGAAT CGCCTGCTTA TGCTGTGACG CCCGGCGCCA GGTACGGATG
    CAGGCCCTGA CATATCTGCA GCGAGCCCTT CTGGTGCATG ACCTACAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TCCTGACCAA GCTTTTGGAA
    AATATCAGCC CTGCAGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG
    CTCTCAAAGG TCTTCCTGCA GCATCTGTCT CCACTGCTGT CACTGTCCAC CTTTGCTGCC
    CTGTGGCTCA CTATCCTAGA CTTCATGGAC AAGTACATGC ATGCAGGTTC CAGTGATTTG
    CTGTCAGAAG CTATCCCCGA GTCTCTGAAG AACATGCTGC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC AAGAGGAGGA GGCCCCTCTG CTCTCTGGGA GATTACCTGG
    GAGCGCATCG ACTGCTTCTT GCCACACTTG CGTGACGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCTGCGGA ACCGCACAGT CAAAAACCTC TGGCCTCTAC TCACCTGACT
    TCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC TCCCAGAGAT ACCCTCAGAA
    ATGGGGGTTT GTGACTCAGA GAAGCCCGAG AGTACCCGAG CTCCCAGCAG CAGCTCCCCC
    GGATCGCCCA TGGCCTCTAG CCCCAGCAAA CTGAGTCCTG CCCAAGAGGG ACCTCCCCCA
    CTGACCCAGC CCCCACTGAT CCTGCAGCCC TTGACCTCCC CACTGCAGGT GGGGGTCCCG
    CCCATGACTC TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC CCCAGTGCCT
    CTCCTAGCCA CACCCCGCCC TACAGACCCC ATCCCCACTT CTGAAGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.