GBF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GBF1
Entrez Gene ID	8729
Full Name	golgi brefeldin A resistant guanine nucleotide exchange factor 1
Synonyms	ARF1GEF
General protein information	Preferred Names golgi brefeldin A resistant guanine nucleotide exchange factor 1 Names Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 BFA-resistant GEF 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q24.32
Summary	This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	603698

mRNA and Protein(s)

mRNA	Protein	Name
XM_005270261.1	XP_005270318.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
XM_006718048.1	XP_006718111.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_006718050.1	XP_006718113.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
XM_017016862.1	XP_016872351.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
XM_011540312.1	XP_011538614.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
XM_006718047.2	XP_006718110.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
XM_017016863.2	XP_016872352.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
XM_011540314.2	XP_011538616.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X11
NM_004193.3	NP_004184.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
XM_017016864.1	XP_016872353.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
NM_001199378.1	NP_001186307.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
NM_001199378.2	NP_001186307.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
NM_001377139.1	NP_001364068.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 6
XM_006718049.1	XP_006718112.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
XM_017016861.1	XP_016872350.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
XM_011540313.1	XP_011538615.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
NM_001199379.1	NP_001186308.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
NM_004193.2	NP_004184.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
XM_006718048.2	XP_006718111.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_006718050.2	XP_006718113.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
XM_017016862.2	XP_016872351.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
XM_011540312.2	XP_011538614.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
XM_011540313.2	XP_011538615.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
NM_001199379.2	NP_001186308.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
NM_001377140.1	NP_001364069.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 7
NM_001377137.1	NP_001364066.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 4
XM_005270261.2	XP_005270318.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
XM_017016864.2	XP_016872353.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
XM_017016863.1	XP_016872352.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
XM_006718047.1	XP_006718110.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
XM_006718049.2	XP_006718112.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
XM_017016861.2	XP_016872350.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
NM_001377138.1	NP_001364067.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 5
NM_001377141.1	NP_001364070.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication.
Farhat R, Ankavay M, Lebsir N, Gouttenoire J, Jackson CL, Wychowski C, Moradpour D, Dubuisson J, Rouill? Y, Cocquerel L
Cellular microbiology20(1)(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GBF1 could regulate different metabolic pathways through the activation of different pairs of Arf proteins.
Title: Identification of class II ADP-ribosylation factors as cellular factors required for hepatitis C virus replication.

Oligomerization appears to stabilize GBF1 in cells.
Title: Oligomerization of the Sec7 domain Arf guanine nucleotide exchange factor GBF1 is dispensable for Golgi localization and function but regulates degradation.

The role of GBF1 in poliovirus replication is independent of its Arf activating function.
Title: Cell-specific establishment of poliovirus resistance to an inhibitor targeting a cellular protein.

Although human rhinovirus 3A protein was previously shown to interact with ACBD3, these data suggest that PI4KIIIbeta recruitment occurred independently of both GBF1 and ACBD3.
Title: GBF1- and ACBD3-independent recruitment of PI4KIII? to replication sites by rhinovirus 3A proteins.

GBF1 plays a critical role early in dengue infection that is independent of its role in the maintenance of Golgi structure.
Title: Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection.

The following GBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu91777	XM_017016864.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu108495	NM_001199379.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu108497	NM_001199378.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu108697	NM_004193.3 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu108697	NM_001377141.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu109403	NM_001377138.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu109463	NM_001377139.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu109500	NM_001377140.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu44657	NM_001377137.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91774	XM_017016861.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91775	XM_017016862.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91776	XM_017016863.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu06003	NM_001199378.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu11757	NM_001199379.1 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu55708	NM_004193.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44653	XM_006718047.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44654	XM_006718048.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44655	XM_006718049.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44656	XM_006718050.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44657	XM_005270261.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu62151	XM_011540312.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu62152	XM_011540313.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu62153	XM_011540314.2 Latest version!	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu44654	XM_006718048.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44656	XM_006718050.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu91775	XM_017016862.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu62151	XM_011540312.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu62152	XM_011540313.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu44657	XM_005270261.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu91777	XM_017016864.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu91776	XM_017016863.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu44653	XM_006718047.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44655	XM_006718049.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu91774	XM_017016861.1	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GBF1 ( XM_017016864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872353.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

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CloneID	OHu91777
Clone ID Related Accession (Same CDS sequence)	XM_017016864.1 , XM_017016864.2
Accession Version	XM_017016864.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC 
CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA 
GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG 
GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG 
GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC 
CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC 
GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT 
ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA 
GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG 
GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA 
GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA 
GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT 
AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA 
AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA 
CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA 
GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG 
GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT 
TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC 
CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG 
CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG 
ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG 
TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA 
CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC 
CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG 
ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG 
GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT 
CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG 
TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT 
GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG 
TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG 
AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG 
GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT 
TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT 
ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA 
GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC 
CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA 
CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG 
GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG 
ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG 
TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG 
GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG 
AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA 
GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC 
CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA 
GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT 
GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC 
GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC 
AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG 
CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC 
CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT 
CCCATACCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872353.1
CDS	2090..5176
Translation	MLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016864.1

ORF Insert Sequence:

ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC 
CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA 
GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG 
GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG 
GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC 
CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC 
GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT 
ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA 
GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG 
GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA 
GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA 
GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT 
AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA 
AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA 
CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA 
GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG 
GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT 
TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC 
CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG 
CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG 
ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG 
TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA 
CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC 
CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG 
ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG 
GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT 
CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG 
TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT 
GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG 
TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG 
AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG 
GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT 
TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT 
ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA 
GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC 
CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA 
CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG 
GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG 
ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG 
TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG 
GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG 
AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA 
GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC 
CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA 
GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT 
GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC 
GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC 
AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG 
CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC 
CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT 
CCCATACCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016864.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872353.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

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CloneID	OHu91777
Clone ID Related Accession (Same CDS sequence)	XM_017016864.1 , XM_017016864.2
Accession Version	XM_017016864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC 
CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA 
GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG 
GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG 
GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC 
CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC 
GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT 
ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA 
GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG 
GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA 
GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA 
GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT 
AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA 
AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA 
CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA 
GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG 
GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT 
TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC 
CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG 
CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG 
ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG 
TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA 
CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC 
CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG 
ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG 
GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT 
CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG 
TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT 
GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG 
TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG 
AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG 
GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT 
TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT 
ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA 
GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC 
CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA 
CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG 
GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG 
ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG 
TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG 
GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG 
AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA 
GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC 
CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA 
GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT 
GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC 
GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC 
AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG 
CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC 
CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT 
CCCATACCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872353.1
CDS	1990..5076
Translation	MLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016864.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016864.2

ORF Insert Sequence:

ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC 
CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA 
GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG 
GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG 
GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC 
CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC 
GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT 
ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA 
GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG 
GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA 
GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA 
GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT 
AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA 
AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA 
CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA 
GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG 
GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT 
TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC 
CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG 
CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG 
ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG 
TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA 
CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC 
CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG 
ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG 
GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT 
CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG 
TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT 
GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG 
TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG 
AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG 
GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT 
TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT 
ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA 
GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC 
CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA 
CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG 
GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG 
ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG 
TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG 
GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG 
AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA 
GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC 
CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA 
GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT 
GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC 
GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC 
AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG 
CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC 
CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT 
CCCATACCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001199379.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186308.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108495
Clone ID Related Accession (Same CDS sequence)	NM_001199379.2
Accession Version	NM_001199379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_001199379.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31632.1, SRR1803614.50084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC 
CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT 
TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA 
CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC 
AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT 
AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG 
CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA 
GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC 
CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC 
ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC 
CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT 
GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG 
GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA 
CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT 
CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG 
ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG 
GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186308.1
CDS	261..5828
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001199379.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199379.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC 
CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT 
TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA 
CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC 
AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT 
AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG 
CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA 
GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC 
CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC 
ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC 
CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT 
GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG 
GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA 
CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT 
CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG 
ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG 
GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001199378.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186307.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

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CloneID	OHu108497
Clone ID Related Accession (Same CDS sequence)	NM_001199378.2
Accession Version	NM_001199378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_001199378.1. Transcript Variant: This variant (2) uses multiple alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK025330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186307.1
CDS	261..5831
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001199378.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199378.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_004193.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004184.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

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CloneID	OHu108697
Clone ID Related Accession (Same CDS sequence)	NM_004193.3 , NM_001377141.1
Accession Version	NM_004193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_004193.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.201996.1, AF068755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004184.1
CDS	261..5840
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_004193.3
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004193.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001377141.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364070.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 8, mRNA.

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CloneID	OHu108697
Clone ID Related Accession (Same CDS sequence)	NM_004193.3 , NM_001377141.1
Accession Version	NM_001377141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Transcript Variant: This variant (8), as well as variant 1, encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.183024.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364070.1
CDS	360..5939
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001377141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377141.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001377138.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364067.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 5, mRNA.

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CloneID	OHu109403
Clone ID Related Accession (Same CDS sequence)	NM_001377138.1
Accession Version	NM_001377138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGTTGCTAG ACCTGATGCA CACCCTGCAC ACGCGGGCAG CCTCTATCTA CAGCTCATGG 
GCGGAGGAGC AACGCCACCT GGAGACAGGT GGCCAGAAGA TTGAAGCTGA TTCTCGCACC 
CTCTGGGCCC ACTGCTGGTG CCCTTTACTG CAGGGTATTG CCTGCCTGTG CTGCGATGCC 
CGGCGCCAGG TACGGATGCA GGCACTGACC TATCTGCAGC GAGCACTACT TGTACATGAT 
CTGCAAAAGC TAGATGCCCT GGAATGGGAG TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA 
CTTACCAAGC TCTTGGAGAA CATCAGCCCT GCAGATGTGG GTGGGATGGA GGAGACCCGG 
ATGAGGGCTT CCACATTGCT CTCTAAGGTC TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA 
CTCTCTACCT TTGCGGCCCT CTGGCTCACC ATCTTGGACT TCATGGACAA GTACATGCAC 
GCAGGCTCCA GCGACTTACT GTCAGAGGCG ATCCCTGAGT CTCTGAAGAA CATGCTTCTG 
GTGATGGACA CAGCGGAGAT TTTCCACAGT GCAGATGCAC GGGGAGGCGG CCCCTCGGCC 
CTCTGGGAGA TCACCTGGGA ACGCATTGAC TGTTTTCTCC CTCACCTACG AGATGAACTC 
TTCAAGCAGA CCGTCATCCA GGACCCCATG CCCATGGAGC CTCAAGGCCA AAAGCCTCTC 
GCCTCAGCCC ACCTGACTTC CGCTGCTGGC GACACTAGGA CACCTGGCCA TCCACCGCCC 
CCAGAGATTC CATCTGAGCT GGGGGCCTGT GACTTTGAGA AGCCCGAGAG CCCCCGAGCC 
GCCAGCAGCA GCTCCCCAGG ATCACCAGTG GCCTCAAGCC CCAGCAGGCT GAGCCCCACC 
CCCGACGGGC CTCCACCCTT GGCTCAGCCC CCACTGATCC TGCAGCCCTT GGCCTCCCCA 
CTGCAGGTGG GCGTGCCACC TATGACTCTG CCCATCATCC TCAACCCTGC GCTCATCGAG 
GCCACCTCAC CAGTGCCCCT CCTGGCCACA CCCCGCCCCA CAGATCCCAT ACCCACCTCT 
GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364067.1
CDS	261..5792
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001377138.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377138.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGTTGCTAG ACCTGATGCA CACCCTGCAC ACGCGGGCAG CCTCTATCTA CAGCTCATGG 
GCGGAGGAGC AACGCCACCT GGAGACAGGT GGCCAGAAGA TTGAAGCTGA TTCTCGCACC 
CTCTGGGCCC ACTGCTGGTG CCCTTTACTG CAGGGTATTG CCTGCCTGTG CTGCGATGCC 
CGGCGCCAGG TACGGATGCA GGCACTGACC TATCTGCAGC GAGCACTACT TGTACATGAT 
CTGCAAAAGC TAGATGCCCT GGAATGGGAG TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA 
CTTACCAAGC TCTTGGAGAA CATCAGCCCT GCAGATGTGG GTGGGATGGA GGAGACCCGG 
ATGAGGGCTT CCACATTGCT CTCTAAGGTC TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA 
CTCTCTACCT TTGCGGCCCT CTGGCTCACC ATCTTGGACT TCATGGACAA GTACATGCAC 
GCAGGCTCCA GCGACTTACT GTCAGAGGCG ATCCCTGAGT CTCTGAAGAA CATGCTTCTG 
GTGATGGACA CAGCGGAGAT TTTCCACAGT GCAGATGCAC GGGGAGGCGG CCCCTCGGCC 
CTCTGGGAGA TCACCTGGGA ACGCATTGAC TGTTTTCTCC CTCACCTACG AGATGAACTC 
TTCAAGCAGA CCGTCATCCA GGACCCCATG CCCATGGAGC CTCAAGGCCA AAAGCCTCTC 
GCCTCAGCCC ACCTGACTTC CGCTGCTGGC GACACTAGGA CACCTGGCCA TCCACCGCCC 
CCAGAGATTC CATCTGAGCT GGGGGCCTGT GACTTTGAGA AGCCCGAGAG CCCCCGAGCC 
GCCAGCAGCA GCTCCCCAGG ATCACCAGTG GCCTCAAGCC CCAGCAGGCT GAGCCCCACC 
CCCGACGGGC CTCCACCCTT GGCTCAGCCC CCACTGATCC TGCAGCCCTT GGCCTCCCCA 
CTGCAGGTGG GCGTGCCACC TATGACTCTG CCCATCATCC TCAACCCTGC GCTCATCGAG 
GCCACCTCAC CAGTGCCCCT CCTGGCCACA CCCCGCCCCA CAGATCCCAT ACCCACCTCT 
GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001377139.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364068.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 6, mRNA.

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CloneID	OHu109463
Clone ID Related Accession (Same CDS sequence)	NM_001377139.1
Accession Version	NM_001377139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT 
GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC 
AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC 
ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364068.1
CDS	261..5546
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001377139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377139.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT 
GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC 
AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC 
ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001377140.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364069.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 7, mRNA.

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CloneID	OHu109500
Clone ID Related Accession (Same CDS sequence)	NM_001377140.1
Accession Version	NM_001377140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.198144.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT 
GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC 
AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC 
ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364069.1
CDS	261..5534
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001377140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377140.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT 
GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC 
AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC 
ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001377137.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364066.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 4, mRNA.

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CloneID	OHu44657
Clone ID Related Accession (Same CDS sequence)	XM_005270261.1 , NM_001377137.1 , XM_005270261.2
Accession Version	NM_001377137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Dec 17, 2019 this sequence version replaced XM_005270261.2. Transcript Variant: This variant (4) encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364066.1
CDS	261..5843
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001377137.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377137.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016861.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872350.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

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CloneID	OHu91774
Clone ID Related Accession (Same CDS sequence)	XM_017016861.1 , XM_017016861.2
Accession Version	XM_017016861.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA 
ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG 
CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC 
ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC 
TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA 
TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA 
GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC 
CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT 
GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC 
ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA 
GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC 
ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG 
ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG 
GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT 
GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA 
ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT 
TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT 
GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA 
CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC 
CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT 
AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG 
ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT 
GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA 
GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA 
AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT 
GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA 
AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA 
GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT 
GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG 
GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA 
GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC 
TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT 
ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT 
CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC 
ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT 
CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC 
CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT 
ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC 
ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT 
GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC 
CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT 
TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG 
CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG 
GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA 
ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG 
GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT 
GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC 
ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT 
TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG 
TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC 
CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA 
GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG 
CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA 
GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC 
TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC 
GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA 
GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA 
TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT 
GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC 
CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA 
TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC 
GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG 
AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA 
GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG 
AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC 
AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC 
TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT 
CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC 
GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA 
CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT 
CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG 
ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG 
CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC 
ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG 
CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC 
TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT 
GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG 
CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA 
CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC 
CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC 
CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC 
TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC 
ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC 
ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872350.1
CDS	295..5952
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016861.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016861.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA 
ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG 
CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC 
ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC 
TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA 
TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA 
GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC 
CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT 
GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC 
ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA 
GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC 
ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG 
ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG 
GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT 
GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA 
ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT 
TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT 
GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA 
CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC 
CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT 
AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG 
ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT 
GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA 
GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA 
AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT 
GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA 
AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA 
GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT 
GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG 
GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA 
GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC 
TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT 
ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT 
CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC 
ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT 
CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC 
CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT 
ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC 
ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT 
GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC 
CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT 
TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG 
CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG 
GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA 
ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG 
GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT 
GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC 
ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT 
TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG 
TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC 
CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA 
GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG 
CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA 
GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC 
TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC 
GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA 
GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA 
TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT 
GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC 
CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA 
TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC 
GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG 
AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA 
GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG 
AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC 
AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC 
TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT 
CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC 
GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA 
CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT 
CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG 
ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG 
CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC 
ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG 
CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC 
TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT 
GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG 
CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA 
CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC 
CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC 
CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC 
TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC 
ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC 
ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016861.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872350.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

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CloneID	OHu91774
Clone ID Related Accession (Same CDS sequence)	XM_017016861.1 , XM_017016861.2
Accession Version	XM_017016861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA 
ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG 
CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC 
ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC 
TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA 
TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA 
GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC 
CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT 
GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC 
ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA 
GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC 
ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG 
ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG 
GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT 
GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA 
ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT 
TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT 
GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA 
CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC 
CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT 
AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG 
ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT 
GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA 
GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA 
AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT 
GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA 
AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA 
GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT 
GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG 
GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA 
GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC 
TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT 
ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT 
CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC 
ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT 
CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC 
CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT 
ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC 
ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT 
GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC 
CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT 
TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG 
CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG 
GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA 
ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG 
GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT 
GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC 
ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT 
TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG 
TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC 
CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA 
GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG 
CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA 
GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC 
TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC 
GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA 
GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA 
TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT 
GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC 
CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA 
TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC 
GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG 
AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA 
GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG 
AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC 
AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC 
TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT 
CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC 
GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA 
CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT 
CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG 
ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG 
CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC 
ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG 
CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC 
TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT 
GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG 
CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA 
CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC 
CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC 
CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC 
TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC 
ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC 
ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872350.1
CDS	262..5919
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016861.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016861.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA 
ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG 
CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC 
ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC 
TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA 
TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA 
GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC 
CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT 
GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC 
ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA 
GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC 
ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG 
ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG 
GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT 
GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA 
ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT 
TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT 
GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA 
CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC 
CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT 
AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG 
ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT 
GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA 
GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA 
AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT 
GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA 
AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA 
GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT 
GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG 
GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA 
GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC 
TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT 
ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT 
CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC 
ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT 
CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC 
CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT 
ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC 
ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT 
GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC 
CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT 
TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG 
CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG 
GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA 
ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG 
GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT 
GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC 
ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT 
TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG 
TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC 
CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA 
GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG 
CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA 
GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC 
TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC 
GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA 
GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA 
TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT 
GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC 
CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA 
TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC 
GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG 
AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA 
GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG 
AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC 
AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC 
TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT 
CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC 
GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA 
CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT 
CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG 
ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG 
CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC 
ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG 
CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC 
TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT 
GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG 
CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA 
CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC 
CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC 
CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC 
TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC 
ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC 
ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872351.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

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CloneID	OHu91775
Clone ID Related Accession (Same CDS sequence)	XM_017016862.1 , XM_017016862.2
Accession Version	XM_017016862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872351.1
CDS	295..5928
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016862.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016862.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872351.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

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CloneID	OHu91775
Clone ID Related Accession (Same CDS sequence)	XM_017016862.1 , XM_017016862.2
Accession Version	XM_017016862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872351.1
CDS	262..5895
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016862.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG 
CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG 
CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA 
CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG 
ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG 
GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC 
CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT 
TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG 
CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA 
GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC 
TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA 
CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016863.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872352.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

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CloneID	OHu91776
Clone ID Related Accession (Same CDS sequence)	XM_017016863.2 , XM_017016863.1
Accession Version	XM_017016863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC 
TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT 
GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG 
TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA 
CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG 
CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG 
GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT 
CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC 
AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG 
AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC 
GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA 
CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC 
CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC 
CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG 
AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG 
CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC 
TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT 
GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC 
ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872352.1
CDS	262..5805
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016863.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016863.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC 
TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT 
GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG 
TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA 
CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG 
CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG 
GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT 
CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC 
AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG 
AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC 
GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA 
CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC 
CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC 
CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG 
AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG 
CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC 
TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT 
GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC 
ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_017016863.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872352.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

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CloneID	OHu91776
Clone ID Related Accession (Same CDS sequence)	XM_017016863.2 , XM_017016863.1
Accession Version	XM_017016863.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC 
TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT 
GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG 
TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA 
CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG 
CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG 
GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT 
CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC 
AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG 
AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC 
GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA 
CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC 
CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC 
CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG 
AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG 
CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC 
TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT 
GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC 
ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872352.1
CDS	295..5838
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_017016863.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016863.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG 
AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG 
CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT 
GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA 
CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG 
AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC 
AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA 
GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT 
TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT 
GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT 
AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG 
CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG 
GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG 
GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC 
TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC 
ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC 
CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG 
AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT 
GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT 
GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA 
TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG 
GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT 
TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA 
GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT 
GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG 
CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG 
GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT 
GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC 
TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC 
TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG 
AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC 
AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC 
AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC 
ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT 
GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG 
GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA 
CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT 
GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA 
TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT 
GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG 
CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC 
CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA 
TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC 
GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA 
CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC 
TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT 
GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG 
TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA 
CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG 
CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG 
GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT 
CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC 
AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG 
AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC 
GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA 
CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC 
CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC 
CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG 
AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG 
CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC 
TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT 
GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC 
ATACCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001199378.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186307.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

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CloneID	OHu06003
Clone ID Related Accession (Same CDS sequence)	NM_001199378.1
Accession Version	NM_001199378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, AK025330.1, AF068755.1, BC007941.2 and AI859700.1. Transcript Variant: This variant (2) uses multiple alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK025330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATC 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186307.1
CDS	295..5865
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001199378.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199378.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATC 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_001199379.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186308.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

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CloneID	OHu11757
Clone ID Related Accession (Same CDS sequence)	NM_001199379.1
Accession Version	NM_001199379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, BC117681.1, AL121928.13, BC007941.2 and AI859700.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.181248.1, SRR1660809.139641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC 
CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT 
TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA 
CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC 
AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT 
AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG 
CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA 
GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC 
CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC 
ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC 
CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT 
GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG 
GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA 
CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT 
CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG 
ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG 
GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186308.1
CDS	295..5862
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001199379.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199379.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC 
CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT 
TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA 
CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC 
AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT 
AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG 
CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA 
GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC 
CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC 
ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC 
CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT 
GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG 
GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA 
CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT 
CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG 
ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG 
GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( NM_004193.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004184.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

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CloneID	OHu55708
Clone ID Related Accession (Same CDS sequence)	NM_004193.2
Accession Version	NM_004193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, AF068755.1 and AI859700.1. On Dec 8, 2010 this sequence version replaced NM_004193.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.173865.1, SRR1660803.8648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004184.1
CDS	295..5874
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_004193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004193.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC 
CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC 
ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT 
CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC 
CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA 
GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC 
TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC 
TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA 
AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC 
AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC 
CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC 
ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA 
GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC 
ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA 
TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA 
CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT 
CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA 
GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC 
AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC 
AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA 
AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC 
TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG 
AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC 
CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG 
CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC 
CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG 
CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC 
CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT 
GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA 
GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT 
GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA 
GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT 
GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG 
CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT 
TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG 
CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC 
TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC 
AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC 
CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC 
GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC 
CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA 
CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC 
TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA 
CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA 
GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC 
TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC 
ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT 
GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC 
GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA 
GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC 
ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC 
GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC 
CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA 
TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC 
TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT 
CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC 
GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA 
GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718047.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718110.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

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CloneID	OHu44653
Clone ID Related Accession (Same CDS sequence)	XM_006718047.2 , XM_006718047.1
Accession Version	XM_006718047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC 
ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG 
ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT 
GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC 
TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG 
CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC 
GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT 
GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG 
GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG 
CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718110.1
CDS	262..5943
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718047.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC 
ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG 
ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT 
GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC 
TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG 
CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC 
GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT 
GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG 
GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG 
CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718047.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718110.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

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CloneID	OHu44653
Clone ID Related Accession (Same CDS sequence)	XM_006718047.2 , XM_006718047.1
Accession Version	XM_006718047.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC 
ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG 
ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT 
GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC 
TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG 
CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC 
GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT 
GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG 
GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG 
CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718110.1
CDS	295..5976
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718047.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC 
ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG 
ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT 
GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC 
TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG 
CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC 
GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT 
GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG 
GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG 
CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718048.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718111.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

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CloneID	OHu44654
Clone ID Related Accession (Same CDS sequence)	XM_006718048.1 , XM_006718048.2
Accession Version	XM_006718048.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA 
GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT 
CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG 
ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA 
GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG 
CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT 
GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC 
TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC 
ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG 
ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA 
ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA 
GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT 
GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA 
GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC 
TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA 
CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT 
GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT 
GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC 
ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG 
GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC 
AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG 
ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT 
CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC 
TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA 
TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC 
AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC 
ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG 
CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT 
GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC 
ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC 
AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG 
AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC 
AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA 
GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA 
CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG 
TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA 
CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT 
GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA 
CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC 
CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG 
CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC 
CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG 
GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT 
CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA 
GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC 
CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC 
TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC 
CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA 
GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT 
TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC 
ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC 
ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC 
TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC 
TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA 
CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC 
ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC 
CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718111.1
CDS	295..5973
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718048.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718048.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA 
GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT 
CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG 
ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA 
GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG 
CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT 
GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC 
TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC 
ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG 
ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA 
ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA 
GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT 
GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA 
GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC 
TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA 
CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT 
GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT 
GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC 
ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG 
GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC 
AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG 
ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT 
CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC 
TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA 
TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC 
AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC 
ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG 
CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT 
GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC 
ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC 
AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG 
AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC 
AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA 
GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA 
CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG 
TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA 
CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT 
GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA 
CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC 
CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG 
CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC 
CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG 
GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT 
CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA 
GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC 
CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC 
TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC 
CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA 
GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT 
TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC 
ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC 
ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC 
TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC 
TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA 
CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC 
ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC 
CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718048.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718111.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

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CloneID	OHu44654
Clone ID Related Accession (Same CDS sequence)	XM_006718048.1 , XM_006718048.2
Accession Version	XM_006718048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA 
GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT 
CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG 
ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA 
GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG 
CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT 
GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC 
TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC 
ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG 
ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA 
ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA 
GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT 
GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA 
GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC 
TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA 
CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT 
GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT 
GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC 
ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG 
GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC 
AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG 
ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT 
CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC 
TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA 
TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC 
AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC 
ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG 
CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT 
GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC 
ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC 
AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG 
AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC 
AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA 
GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA 
CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG 
TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA 
CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT 
GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA 
CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC 
CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG 
CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC 
CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG 
GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT 
CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA 
GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC 
CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC 
TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC 
CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA 
GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT 
TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC 
ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC 
ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC 
TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC 
TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA 
CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC 
ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC 
CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718111.1
CDS	262..5940
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718048.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718048.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA 
GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT 
CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG 
ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA 
GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG 
CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT 
GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC 
TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC 
ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG 
ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA 
ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA 
GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT 
GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA 
GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC 
TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA 
CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT 
GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT 
GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC 
ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG 
GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC 
AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG 
ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT 
CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC 
TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA 
TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC 
AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC 
ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG 
CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT 
GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC 
ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC 
AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG 
AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC 
AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA 
GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA 
CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG 
TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA 
CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT 
GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA 
CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC 
CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG 
CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC 
CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG 
GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT 
CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA 
GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC 
CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC 
TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC 
CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA 
GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT 
TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC 
ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC 
ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC 
TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC 
TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA 
CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC 
ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC 
CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718049.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718112.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

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CloneID	OHu44655
Clone ID Related Accession (Same CDS sequence)	XM_006718049.1 , XM_006718049.2
Accession Version	XM_006718049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC 
TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT 
GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC 
TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA 
GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC 
AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT 
GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC 
CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC 
ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT 
CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG 
GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT 
CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT 
CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA 
CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG 
CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC 
AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG 
CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC 
AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA 
GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718112.1
CDS	295..5964
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718049.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC 
TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT 
GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC 
TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA 
GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC 
AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT 
GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC 
CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC 
ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT 
CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG 
GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT 
CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT 
CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA 
CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG 
CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC 
AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG 
CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC 
AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA 
GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718049.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718112.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

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CloneID	OHu44655
Clone ID Related Accession (Same CDS sequence)	XM_006718049.1 , XM_006718049.2
Accession Version	XM_006718049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC 
TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT 
GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC 
TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA 
GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC 
AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT 
GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC 
CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC 
ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT 
CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG 
GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT 
CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT 
CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA 
CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG 
CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC 
AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG 
CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC 
AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA 
GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718112.1
CDS	262..5931
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718049.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC 
TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT 
GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC 
TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA 
GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC 
AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT 
GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC 
CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC 
ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT 
CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG 
GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT 
CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT 
CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA 
CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG 
CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC 
AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG 
CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC 
AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA 
GATCCCATAC CCACCTCTGA GGTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718050.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718113.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

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CloneID	OHu44656
Clone ID Related Accession (Same CDS sequence)	XM_006718050.1 , XM_006718050.2
Accession Version	XM_006718050.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718113.1
CDS	295..5940
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718050.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718050.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_006718050.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718113.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

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CloneID	OHu44656
Clone ID Related Accession (Same CDS sequence)	XM_006718050.1 , XM_006718050.2
Accession Version	XM_006718050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718113.1
CDS	262..5907
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSSERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006718050.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718050.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC 
TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA 
TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG 
AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC 
AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT 
CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG 
TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC 
AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG 
GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC 
CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC 
AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC 
AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG 
AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC 
ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT 
GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT 
ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC 
TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC 
AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC 
ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA 
TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT 
CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC 
CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC 
AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC 
TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT 
GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA 
ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG 
GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG 
ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT 
CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG 
GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT 
CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC 
AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC 
TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG 
CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA 
GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC 
GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT 
GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG 
CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA 
CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC 
CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG 
CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC 
CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC 
AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG 
GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC 
TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG 
GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG 
GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG 
CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA 
GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG 
ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC 
AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC 
GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG 
GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005270261.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270318.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

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CloneID	OHu44657
Clone ID Related Accession (Same CDS sequence)	XM_005270261.1 , NM_001377137.1 , XM_005270261.2
Accession Version	XM_005270261.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270318.1
CDS	295..5877
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_005270261.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270261.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005270261.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270318.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

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CloneID	OHu44657
Clone ID Related Accession (Same CDS sequence)	XM_005270261.1 , NM_001377137.1 , XM_005270261.2
Accession Version	XM_005270261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005270261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270318.1
CDS	262..5844
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_005270261.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270261.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC 
ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG 
ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC 
CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC 
ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT 
ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG 
ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG 
TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT 
GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC 
CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG 
TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT 
CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA 
TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC 
AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC 
ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT 
GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC 
CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC 
CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA 
GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA 
GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG 
CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG 
TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC 
GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC 
CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT 
GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT 
GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT 
GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA 
AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG 
CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA 
GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG 
GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC 
CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA 
GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT 
CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT 
AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC 
TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC 
GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA 
GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG 
GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT 
GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG 
ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT 
CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG 
GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA 
GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG 
CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC 
CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG 
GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG 
CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT 
TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC 
TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC 
AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC 
ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG 
ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG 
ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC 
CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT 
CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC 
AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG 
CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG 
GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA 
CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_011540312.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538614.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

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CloneID	OHu62151
Clone ID Related Accession (Same CDS sequence)	XM_011540312.1 , XM_011540312.2
Accession Version	XM_011540312.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC 
AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA 
ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA 
CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC 
ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG 
TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT 
CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG 
GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT 
GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT 
AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG 
TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG 
GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG 
GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA 
TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC 
ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA 
GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG 
TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG 
AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC 
CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC 
ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC 
GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG 
TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT 
CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA 
TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG 
CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT 
GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT 
GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC 
TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA 
GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT 
GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT 
GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC 
GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT 
GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT 
CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC 
CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC 
GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC 
AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG 
GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC 
TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC 
AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG 
CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC 
AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT 
TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC 
TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT 
GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG 
TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC 
ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC 
CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA 
GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA 
AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT 
CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC 
CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG 
AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG 
CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA 
CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538614.1
CDS	295..5835
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTDKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_011540312.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540312.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC 
AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA 
ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA 
CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC 
ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG 
TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT 
CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG 
GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT 
GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT 
AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG 
TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG 
GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG 
GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA 
TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC 
ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA 
GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG 
TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG 
AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC 
CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC 
ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC 
GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG 
TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT 
CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA 
TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG 
CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT 
GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT 
GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC 
TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA 
GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT 
GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT 
GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC 
GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT 
GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT 
CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC 
CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC 
GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC 
AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG 
GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC 
TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC 
AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG 
CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC 
AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT 
TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC 
TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT 
GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG 
TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC 
ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC 
CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA 
GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA 
AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT 
CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC 
CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG 
AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG 
CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA 
CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_011540312.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538614.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

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CloneID	OHu62151
Clone ID Related Accession (Same CDS sequence)	XM_011540312.1 , XM_011540312.2
Accession Version	XM_011540312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC 
AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA 
ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA 
CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC 
ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG 
TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT 
CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG 
GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT 
GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT 
AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG 
TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG 
GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG 
GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA 
TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC 
ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA 
GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG 
TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG 
AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC 
CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC 
ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC 
GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG 
TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT 
CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA 
TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG 
CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT 
GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT 
GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC 
TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA 
GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT 
GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT 
GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC 
GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT 
GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT 
CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC 
CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC 
GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC 
AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG 
GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC 
TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC 
AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG 
CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC 
AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT 
TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC 
TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT 
GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG 
TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC 
ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC 
CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA 
GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA 
AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT 
CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC 
CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG 
AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG 
CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA 
CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538614.1
CDS	262..5802
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTDKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_011540312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540312.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC 
AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA 
ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA 
CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC 
ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG 
TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT 
CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG 
GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT 
GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT 
AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG 
TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG 
GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG 
GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA 
TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC 
ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA 
GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG 
TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG 
AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC 
CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC 
ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC 
GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG 
TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT 
CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA 
TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG 
CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT 
GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT 
GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC 
TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA 
GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT 
GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT 
GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC 
GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT 
GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT 
CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC 
CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC 
GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC 
AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG 
GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC 
TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC 
AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG 
CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC 
AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT 
TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC 
TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT 
GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG 
TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC 
ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC 
CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA 
GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA 
AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT 
CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC 
CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG 
AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG 
CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA 
CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_011540313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538615.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

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CloneID	OHu62152
Clone ID Related Accession (Same CDS sequence)	XM_011540313.1 , XM_011540313.2
Accession Version	XM_011540313.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538615.1
CDS	295..5211
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVGLPTGLKIKFKS

Target ORF information:

RefSeq Version	XM_011540313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540313.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_011540313.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538615.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

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CloneID	OHu62152
Clone ID Related Accession (Same CDS sequence)	XM_011540313.1 , XM_011540313.2
Accession Version	XM_011540313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538615.1
CDS	262..5178
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKISPCLLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVGLPTGLKIKFKS

Target ORF information:

RefSeq Version	XM_011540313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540313.2

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG 
CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT 
TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA 
CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC 
AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG 
AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA 
GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC 
ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT 
CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT 
GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG 
CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG 
TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG 
CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG 
ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC 
CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC 
ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC 
ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC 
ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC 
TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG 
TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC 
AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG 
GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG 
AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG 
CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA 
GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC 
ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC 
CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG 
GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC 
TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG 
GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA 
GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG 
GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT 
GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG 
CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA 
GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC 
TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT 
GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT 
CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT 
ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT 
GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG 
GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG 
ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG 
AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG 
GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT 
TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC 
CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC 
TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA 
GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT 
CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT 
GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG 
TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT 
TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT 
AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC 
ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG 
AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC 
CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC 
ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT 
GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG 
CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC 
TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_011540314.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538616.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X12, mRNA.

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CloneID	OHu62153
Clone ID Related Accession (Same CDS sequence)	XM_011540314.2
Accession Version	XM_011540314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATGGA TTACGTCAAT CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG 
CTACTCAGCA TAGAGCGACT AAACCTTTAT GCTGCTTCCC TGCGAGTATG CTTCCTACTG 
TTTGAGAGCA TGCGAGAGCA CCTCAAGTTC CAAATGGAGA TGTACATCAA AAAGCTTATG 
GAGATCATCA CTGTGGAGAA CCCCAAGATG CCTTATGAGA TGAAGGAGAT GGCACTGGAG 
GCCATTGTGC AGCTCTGGCG CATCCCCAGC TTTGTCACAG AGCTCTACAT CAACTATGAT 
TGTGACTACT ACTGTTCCAA CCTCTTTGAG GAACTCACAA AGCTGCTGTC CAAGAATGCC 
TTCCCTGTGT CTGGTCAACT CTATACAACA CACCTACTAT CTCTTGATGC CCTATTGACA 
GTGATTGACA GCACCGAGGC CCACTGCCAG GCTAAAGTCC TCAACAGCCT CACCCAGCAA 
GAGAAGAAGG AGACAGCCAG ACCAAGCTGT GAGATAGTAG ATGGCACCCG AGAAGCTAGC 
AATACTGAGA GAACTGCCAG CGATGGGAAA GCTGTAGGCA TGGCCTCAGA CATCCCAGGC 
CTGCATCTGC CAGGTGGAGG GCGGCTGCCA CCAGAACATG GGAAATCAGG ATGCAGTGAT 
CTGGAGGAAG CTGTTGACTC TGGGGCTGAC AAAAAGTTTG CCCGGAAGCC ACCCCGATTT 
TCCTGTCTCC TGCCAGATCC ACGGGAACTA ATTGAAATTA AAAACAAAAA GAAGCTGCTA 
ATCACTGGCA CAGAGCAGTT CAATCAGAAA CCAAAGAAGG GGATTCAGTT TCTGCAAGAG 
AAAGGCCTCC TCACCATCCC AATGGACAAC ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC 
CCTCGGCTGG ACAAGAAGAT GATTGGAGAG TTTGTGAGTG ACCGCAAAAA CATTGACCTG 
TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT CAGGGTCTGC GACTGGACGA AGCCCTCCGC 
CTCTACCTGG AAGCCTTCCG TTTGCCTGGG GAAGCACCAG TCATCCAGAG GTTGCTGGAG 
GCATTCACAG AGCGTTGGAT GAATTGTAAT GGCTCCCCAT TTGCCAATAG CGATGCCTGC 
TTTTCCCTGG CCTATGCTGT CATCATGCTT AATACTGACC AGCACAACCA CAATGTTCGT 
AAACAGAATG CACCCATGAC CCTGGAGGAG TTTCGCAAAA ATCTGAAAGG TGTGAATGGA 
GGCAAGGACT TTGAGCAAGA CATCCTGGAG GACATGTACC ATGCCATCAA GAATGAGGAA 
ATTGTAATGC CTGAGGAGCA GACAGGCTTG GTTCGGGAGA ACTATGTGTG GAATGTGCTG 
CTTCATCGAG GTGCCACCCC TGAGGGCATA TTCCTGCGTG TGCCTACTGC CAGCTATGAT 
CTTGACCTCT TCACCATGAC CTGGGGCCCC ACTATTGCTG CTCTCTCTTA TGTCTTTGAC 
AAAAGCCTTG AGGAGACAAT CATCCAGAAA GCCATCTCAG GCTTCAGGAA GTGCGCCATG 
ATCTCCGCCC ACTATGGCCT CAGCGATGTG TTTGACAATC TCATCATCTC TCTATGCAAA 
TTCACAGCTC TCAGCAGTGA GTCTATTGAG AACCTGCCCA GTGTATTTGG AAGCAACCCT 
AAAGCCCATA TTGCAGCCAA GACAGTATTC CATTTGGCCC ATCGTCATGG TGACATCCTG 
CGGGAGGGCT GGAAGAATAT CATGGAGGCC ATGCTGCAGC TCTTCCGAGC CCAACTACTG 
CCCAAGGCTA TGATAGAGGT AGAAGATTTC GTGGATCCCA ATGGCAAGAT CTCTCTACAG 
CGGGAAGAGA CACCATCAAA CCGAGGAGAG TCAACAGTGC TGAGCTTTGT GAGCTGGCTA 
ACACTGAGTG GTCCTGAGCA GTCTAGTGTT CGGGGCCCAT CCACTGAAAA CCAAGAGGCC 
AAGAGAGTGG CCTTAGAGTG TATAAAGCAA TGTGACCCAG AAAAAATGAT CACAGAAAGC 
AAGTTCCTCC AGCTGGAGTC ACTACAGGAG CTCATGAAGG CTCTGGTCTC AGTGACACCA 
GATGAAGAGA CATATGATGA GGAAGATGCT GCTTTCTGCC TAGAGATGCT GCTAAGGATT 
GTGTTGGAGA ACAGGGATCG TGTGGGCTGT GTGTGGCAGA CTGTTCGAGA CCATCTATAC 
CACCTCTGTG TTCAGGCACA AGATTTCTGC TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG 
CTACGCCTGG CCATTCGGCT TCTCCGGAGA GAAGAGATCA GTGCTCAGGT GCTGCTCTCC 
CTGCGCATTT TGCTACTGAT GAAGCCCAGT GTGCTATCCC GAGTCAGCCA CCAGGTTGCG 
TATGGGCTCC ATGAACTCCT GAAGACCAAT GCAGCCAACA TCCACTCAGG TGATGACTGG 
GCCACACTCT TCACACTGCT GGAGTGCATC GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG 
CAGGCCACAG CCAGGGCAGA TGCACCTGAT GCCGGGGCCC AGTCAGATAG TGAGCTCCCA 
TCCTACCATC AGAATGACGT GAGCCTGGAT CGAGGGTACA CTTCCGACTC AGAGGTCTAC 
ACTGACCATG GCAGGCCGGG CAAGATACAC CGATCAGCCA CAGATGCCGA TGTGGTCAAC 
AGTGGTTGGT TAGTGGTTGG GAAGGATGAC GTTGATAACT CCAAGCCAGG GCCCAGCCGC 
CCAGGCCCTT CACCCCTGAT CAATCAATAC AGCCTAACAG TGGGACTGGA TTTGGGGCCA 
CACGACACTA AGTCTCTGCT TAAGTGTGTG GAATCGCTGT CCTTCATTGT GCGTGATGCT 
GCCCACATCA CACCTGACAA CTTTGAGCTC TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG 
GCCAGTCTGA ATGGCGGATG CAAGTCCCAG GAGAAACGTG GCAAGAGTCA CAAATATGAC 
AGCAAAGGGA ACCGCTTCAA GAAGAAATCC AAAGAGGGAT CAATGCTTCG CCGGCCTCGA 
ACCTCCAGCC AACATGCCTC TCGGGGCGGG CAGAGTGATG ATGATGAGGA CGAAGGCGTG 
CCTGCCAGCT ACCATACGGT GTCTTTACAG GTCAGTCAGG ACTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538616.1
CDS	104..4354
Translation	MTWITSIPGACALHSPPRKKLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_011540314.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540314.2

ORF Insert Sequence:

ATGACATGGA TTACGTCAAT CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG 
CTACTCAGCA TAGAGCGACT AAACCTTTAT GCTGCTTCCC TGCGAGTATG CTTCCTACTG 
TTTGAGAGCA TGCGAGAGCA CCTCAAGTTC CAAATGGAGA TGTACATCAA AAAGCTTATG 
GAGATCATCA CTGTGGAGAA CCCCAAGATG CCTTATGAGA TGAAGGAGAT GGCACTGGAG 
GCCATTGTGC AGCTCTGGCG CATCCCCAGC TTTGTCACAG AGCTCTACAT CAACTATGAT 
TGTGACTACT ACTGTTCCAA CCTCTTTGAG GAACTCACAA AGCTGCTGTC CAAGAATGCC 
TTCCCTGTGT CTGGTCAACT CTATACAACA CACCTACTAT CTCTTGATGC CCTATTGACA 
GTGATTGACA GCACCGAGGC CCACTGCCAG GCTAAAGTCC TCAACAGCCT CACCCAGCAA 
GAGAAGAAGG AGACAGCCAG ACCAAGCTGT GAGATAGTAG ATGGCACCCG AGAAGCTAGC 
AATACTGAGA GAACTGCCAG CGATGGGAAA GCTGTAGGCA TGGCCTCAGA CATCCCAGGC 
CTGCATCTGC CAGGTGGAGG GCGGCTGCCA CCAGAACATG GGAAATCAGG ATGCAGTGAT 
CTGGAGGAAG CTGTTGACTC TGGGGCTGAC AAAAAGTTTG CCCGGAAGCC ACCCCGATTT 
TCCTGTCTCC TGCCAGATCC ACGGGAACTA ATTGAAATTA AAAACAAAAA GAAGCTGCTA 
ATCACTGGCA CAGAGCAGTT CAATCAGAAA CCAAAGAAGG GGATTCAGTT TCTGCAAGAG 
AAAGGCCTCC TCACCATCCC AATGGACAAC ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC 
CCTCGGCTGG ACAAGAAGAT GATTGGAGAG TTTGTGAGTG ACCGCAAAAA CATTGACCTG 
TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT CAGGGTCTGC GACTGGACGA AGCCCTCCGC 
CTCTACCTGG AAGCCTTCCG TTTGCCTGGG GAAGCACCAG TCATCCAGAG GTTGCTGGAG 
GCATTCACAG AGCGTTGGAT GAATTGTAAT GGCTCCCCAT TTGCCAATAG CGATGCCTGC 
TTTTCCCTGG CCTATGCTGT CATCATGCTT AATACTGACC AGCACAACCA CAATGTTCGT 
AAACAGAATG CACCCATGAC CCTGGAGGAG TTTCGCAAAA ATCTGAAAGG TGTGAATGGA 
GGCAAGGACT TTGAGCAAGA CATCCTGGAG GACATGTACC ATGCCATCAA GAATGAGGAA 
ATTGTAATGC CTGAGGAGCA GACAGGCTTG GTTCGGGAGA ACTATGTGTG GAATGTGCTG 
CTTCATCGAG GTGCCACCCC TGAGGGCATA TTCCTGCGTG TGCCTACTGC CAGCTATGAT 
CTTGACCTCT TCACCATGAC CTGGGGCCCC ACTATTGCTG CTCTCTCTTA TGTCTTTGAC 
AAAAGCCTTG AGGAGACAAT CATCCAGAAA GCCATCTCAG GCTTCAGGAA GTGCGCCATG 
ATCTCCGCCC ACTATGGCCT CAGCGATGTG TTTGACAATC TCATCATCTC TCTATGCAAA 
TTCACAGCTC TCAGCAGTGA GTCTATTGAG AACCTGCCCA GTGTATTTGG AAGCAACCCT 
AAAGCCCATA TTGCAGCCAA GACAGTATTC CATTTGGCCC ATCGTCATGG TGACATCCTG 
CGGGAGGGCT GGAAGAATAT CATGGAGGCC ATGCTGCAGC TCTTCCGAGC CCAACTACTG 
CCCAAGGCTA TGATAGAGGT AGAAGATTTC GTGGATCCCA ATGGCAAGAT CTCTCTACAG 
CGGGAAGAGA CACCATCAAA CCGAGGAGAG TCAACAGTGC TGAGCTTTGT GAGCTGGCTA 
ACACTGAGTG GTCCTGAGCA GTCTAGTGTT CGGGGCCCAT CCACTGAAAA CCAAGAGGCC 
AAGAGAGTGG CCTTAGAGTG TATAAAGCAA TGTGACCCAG AAAAAATGAT CACAGAAAGC 
AAGTTCCTCC AGCTGGAGTC ACTACAGGAG CTCATGAAGG CTCTGGTCTC AGTGACACCA 
GATGAAGAGA CATATGATGA GGAAGATGCT GCTTTCTGCC TAGAGATGCT GCTAAGGATT 
GTGTTGGAGA ACAGGGATCG TGTGGGCTGT GTGTGGCAGA CTGTTCGAGA CCATCTATAC 
CACCTCTGTG TTCAGGCACA AGATTTCTGC TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG 
CTACGCCTGG CCATTCGGCT TCTCCGGAGA GAAGAGATCA GTGCTCAGGT GCTGCTCTCC 
CTGCGCATTT TGCTACTGAT GAAGCCCAGT GTGCTATCCC GAGTCAGCCA CCAGGTTGCG 
TATGGGCTCC ATGAACTCCT GAAGACCAAT GCAGCCAACA TCCACTCAGG TGATGACTGG 
GCCACACTCT TCACACTGCT GGAGTGCATC GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG 
CAGGCCACAG CCAGGGCAGA TGCACCTGAT GCCGGGGCCC AGTCAGATAG TGAGCTCCCA 
TCCTACCATC AGAATGACGT GAGCCTGGAT CGAGGGTACA CTTCCGACTC AGAGGTCTAC 
ACTGACCATG GCAGGCCGGG CAAGATACAC CGATCAGCCA CAGATGCCGA TGTGGTCAAC 
AGTGGTTGGT TAGTGGTTGG GAAGGATGAC GTTGATAACT CCAAGCCAGG GCCCAGCCGC 
CCAGGCCCTT CACCCCTGAT CAATCAATAC AGCCTAACAG TGGGACTGGA TTTGGGGCCA 
CACGACACTA AGTCTCTGCT TAAGTGTGTG GAATCGCTGT CCTTCATTGT GCGTGATGCT 
GCCCACATCA CACCTGACAA CTTTGAGCTC TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG 
GCCAGTCTGA ATGGCGGATG CAAGTCCCAG GAGAAACGTG GCAAGAGTCA CAAATATGAC 
AGCAAAGGGA ACCGCTTCAA GAAGAAATCC AAAGAGGGAT CAATGCTTCG CCGGCCTCGA 
ACCTCCAGCC AACATGCCTC TCGGGGCGGG CAGAGTGATG ATGATGAGGA CGAAGGCGTG 
CCTGCCAGCT ACCATACGGT GTCTTTACAG GTCAGTCAGG ACTTGCTAGA CCTGATGCAC 
ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG 
GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC 
CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG 
GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG 
GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC 
ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC 
TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC 
TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG 
TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT 
TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA 
CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG 
GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC 
GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG 
GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA 
TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG 
GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT 
ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC 
CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication.
Cellular microbiology20(1)(2018 Jan)
Farhat R,Ankavay M,Lebsir N,Gouttenoire J,Jackson CL,Wychowski C,Moradpour D,Dubuisson J,Rouill? Y,Cocquerel L

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GBF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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