Gbf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gbf1
Entrez Gene ID	309451
Full Name	golgi brefeldin A resistant guanine nucleotide exchange factor 1
Synonyms	RGD1307160
General protein information	Preferred Names golgi brefeldin A resistant guanine nucleotide exchange factor 1 Names Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1 golgi-specific brefeldin A-resistance factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231497.3	XP_006231559.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_006231498.3	XP_006231560.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
XM_006231502.3	XP_006231564.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
NM_001191634.1	NP_001178563.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
XM_006231501.3	XP_006231563.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
XM_006231500.3	XP_006231562.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
XM_006231496.3	XP_006231558.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M.
Hansen MD, Johnsen IB, Stiberg KA, Sherstova T, Wakita T, Richard GM, Kandasamy RK, Meurs EF, Anthonsen MW
Proceedings of the National Academy of Sciences of the United States of America114(17)E3462-E3471(2017 Apr)

The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).
Lowery J, Szul T, Styers M, Holloway Z, Oorschot V, Klumperman J, Sztul E
The Journal of biological chemistry288(16)11532-45(2013 Apr)

AMPK phosphorylates GBF1 for mitotic Golgi disassembly.
Mao L, Li N, Guo Y, Xu X, Gao L, Xu Y, Zhou L, Liu W
Journal of cell science126(Pt 6)1498-505(2013 Mar)

GBF1 bears a novel phosphatidylinositol-phosphate binding module, BP3K, to link PI3K? activity with Arf1 activation involved in GPCR-mediated neutrophil chemotaxis and superoxide production.
Mazaki Y, Nishimura Y, Sabe H
Molecular biology of the cell23(13)2457-67(2012 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07293	NM_001191634.1 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24164	XM_006231496.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24165	XM_006231497.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24166	XM_006231498.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24168	XM_006231500.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24169	XM_006231501.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24170	XM_006231502.3 Latest version!	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gbf1 ( NM_001191634.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178563.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), mRNA.

Price & Availability↑

CloneID	ORa07293
Clone ID Related Accession (Same CDS sequence)	NM_001191634.1
Accession Version	NM_001191634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC119478.9 and AC096600.7. On Jul 17, 2010 this sequence version replaced XM_219953.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GGAAGGGGCC 
CATGTAGAAA AGGCCCAGTC AGCATCGGTG GAATCTATCC CTGAAGTGCT AGAGGAGTGC 
ACATCCCCTA CTGACCATTC TGACTCTGCC TCTGTCCATG ACATGGATTA TGTCAATCCT 
CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT 
CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACATGACCGC 
CATAACTCTG AGGTTATGAT CCACATGGGA CTTCATTTGC TGACAGTAGC TCTTGAGTCA 
GCCCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATCA AGGATGAGAT GTGCCGACAC 
TTATTCCAGC TACTCAGCGT AGAGCGGCTG AACCTTTACG CTGCTTCCCT ACGAGTGTGC 
TTTTTGCTCT TTGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTGGAGAT GTACATCAAA 
AAGCTCATGG AGATTATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCGCTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TCGTCACGGA GCTCTACATC 
AACTATGACT GTGACTACTA CTGCTCCAAC CTCTTCGAAG ACCTCACTAA GCTGCTGTCC 
AAGAATGCCT TTCCTGTGTC TGGCCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC 
CTATTGACAG TTATTGACAG CACTGAGGCT CATTGTCAGG CCAAAGTCCT CAACACCCTC 
AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAGCCCA 
GACACTTACA AGTCTGAGAG AGCAGCCAGT GATGGGAAAG CCACAGGAGT GCCCTCAGAT 
GCTCCAGGCC TTCATTTTTC AAGTGGAGGG TGGTTGTCAA CAGAACATGG AAAGCCAGGG 
TGCAGTGATC TGGAGGAAGC TGGTGACTCT GGGGTTGACA AAAAGACCAC CAGGAAGCCA 
CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA 
AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAAAACC CTCGGCTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTCC AGGGTCTGAG GCTTGATGAG 
GCTCTCCGCC TCTACCTAGA AGCCTTCCGT CTGCCTGGGG AAGCGCCTGT TATTCACAGG 
CTGCTGGAGG TGTTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGC 
GATGCCTGCT TTGCCCTGGC CTATGCTGTT ATCATGCTTA ACACTGACCA ACATAACCAC 
AATGTTCGCA AGCAAAATGC ACCCATGACC CTGGAGGAGT TCCGCAAAAA CCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAGGAACAA ACAGGTCTGG TTCGTGAGAA TTATGTGTGG 
AGTGTGCTGC TTCATCGAGG TGCTACCCCT GAGGGCATCT TCCTTCGTGT ACCCCCTGGC 
AGCTATGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTGTTCGATA AAAGCCTCGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTCGGA 
AGCAACCCTA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTTTTAC CGAAGGCTAT GGTAGAGGTA GAAGATTTTG TGGATCCCAA TGGTAAGATC 
TCTCTACAGC GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTCCT TAGTTTTGTA 
AGCTGGCTGA CACTGAGTGG TCCTGAGCAG TCCAGTGTCC GGGGCCCATC GACAGAGAAC 
CAAGAGGCCA AGCGAGTAGC CTTGGACTGC ATCAAGCAAT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAATCT CTGCAGGAGC TCATGAAGGC TCTGGTCTCG 
GTGACACCAG ATGAAGAGAC ATATGATGAA GAAGATGCTG CTTTCTGTCT GGAGATGCTG 
CTAAGGATTG TGTTAGAAAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT 
CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG 
GTAGGGCTGC TACGCCTAGC CATTCGGCTA CTCCGAAGAG AAGAGATTAG TGGCCAGGTC 
CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC 
CAGGTCGCTT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT 
GATGACTGGG CCACTCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA 
GATGCTCTAC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CTGGAGCACA ATCCGACAGT 
GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTAGACC GAGGGTACAC TTCCGACTCA 
GAAGTCTACA CTGACCATGG CAGGCCTGGC AAGATGCACC GATCAGCCAC AGACGCTGAC 
ATGGCCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGCA 
GCAGGACTCA GCAGGCCAGG CCCTTCGCCC TTGGTCAATC AGTACAGCCT TACAGTGGGC 
CTGGACCTGG GACCACATGA CACTAAGTCC TTGCTTAAGT GTGTGGAGTC TCTGTCCTTC 
ATTGTTCGTG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACCCTC 
CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGTGGCAAG 
AGTCATAAAT ATGACAGCAA AGGGAACCGG TTCAAGAAAA AGCCCAAGGA GGGCTCGATG 
CTTCGGCGGC CCCGGGCATC CAGCCAGCAT GCCACTCGGG CTGGACACAG TGATGAAGAG 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACACGG GCAGCCTCTA TCTACAGTTC ATGGGCAGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCGG AAGATTGAAG CTGACTCACG TACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ATTACAAGGT ATCGCTTGCT TATGCTGTGA CGCCCGGCGC 
CAGGTGCGGA TGCAGGCCCT GACATATCTC CAGCGAGCCC TTCTGGTACA TGACCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC GCTACTGACC 
AAGCTTTTAG AAAATATCAG CCCTGCGGAT GTGGGCGGGA TGGAGGAGAC CCGGATGCGG 
GCTTCCACAC TGCTCTCAAA GGTCTTCCTG CAGCACCTGT CCCCACTGCT GTCCCTGTCC 
ACCTTTGCTG CCCTGTGGCT CACCATCCTG GACTTCATGG ACAAGTACAT GCACGCAGGT 
TCCAGTGACT TGCTGTCAGA AGCTGTCCCT GAGTCTCTGA AGAACATGCT CCTTGTGATG 
GACACAGCAG AGATTTTCCA CAGTGCAGAT GCAAGGGGAG GCAGCCCCTC TGCTCTCTGG 
GAGATCACCT GGGAGCGCAT CGATTGCTTC TTACCACACC TACGTGATGA ACTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCCACA GAGCCTCACA GTCAAAAACC TCTGGCTTCT 
ACTCACCTGA CGCCTGCTGC TGGTGACCCC AGGATGCCTG GCCATCCACC TCCTCCAGAG 
ATACCCTCAG AAGTGGGAGC TTGTGACCCG GAGAAGCCCG AGAGCACCAG AGCTGCCAGC 
AGCAGCTCCC CTGGATCGCC GGTGGCCTCT AGCCCCAGCA AACAGAGTCC TGCCCCAGAG 
GGACCTCCCC CACTGGCCCA GCCCCCACTA ATCCTACAGC CCTTGACCTC CCCACTGCAG 
GTGGGGGTAC CACCCATGAC TTTGCCCATC ATCCTCAACC CTGCACTCAT TGAGGCCACC 
TCTCCAGTGC CTCTCTTAGC CACGCCACGC CCTACAGACC CCATCCCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178563.1
CDS	207..5792
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	NM_001191634.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191634.1

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GGAAGGGGCC 
CATGTAGAAA AGGCCCAGTC AGCATCGGTG GAATCTATCC CTGAAGTGCT AGAGGAGTGC 
ACATCCCCTA CTGACCATTC TGACTCTGCC TCTGTCCATG ACATGGATTA TGTCAATCCT 
CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT 
CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACATGACCGC 
CATAACTCTG AGGTTATGAT CCACATGGGA CTTCATTTGC TGACAGTAGC TCTTGAGTCA 
GCCCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATCA AGGATGAGAT GTGCCGACAC 
TTATTCCAGC TACTCAGCGT AGAGCGGCTG AACCTTTACG CTGCTTCCCT ACGAGTGTGC 
TTTTTGCTCT TTGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTGGAGAT GTACATCAAA 
AAGCTCATGG AGATTATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCGCTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TCGTCACGGA GCTCTACATC 
AACTATGACT GTGACTACTA CTGCTCCAAC CTCTTCGAAG ACCTCACTAA GCTGCTGTCC 
AAGAATGCCT TTCCTGTGTC TGGCCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC 
CTATTGACAG TTATTGACAG CACTGAGGCT CATTGTCAGG CCAAAGTCCT CAACACCCTC 
AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAGCCCA 
GACACTTACA AGTCTGAGAG AGCAGCCAGT GATGGGAAAG CCACAGGAGT GCCCTCAGAT 
GCTCCAGGCC TTCATTTTTC AAGTGGAGGG TGGTTGTCAA CAGAACATGG AAAGCCAGGG 
TGCAGTGATC TGGAGGAAGC TGGTGACTCT GGGGTTGACA AAAAGACCAC CAGGAAGCCA 
CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA 
AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAAAACC CTCGGCTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTCC AGGGTCTGAG GCTTGATGAG 
GCTCTCCGCC TCTACCTAGA AGCCTTCCGT CTGCCTGGGG AAGCGCCTGT TATTCACAGG 
CTGCTGGAGG TGTTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGC 
GATGCCTGCT TTGCCCTGGC CTATGCTGTT ATCATGCTTA ACACTGACCA ACATAACCAC 
AATGTTCGCA AGCAAAATGC ACCCATGACC CTGGAGGAGT TCCGCAAAAA CCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAGGAACAA ACAGGTCTGG TTCGTGAGAA TTATGTGTGG 
AGTGTGCTGC TTCATCGAGG TGCTACCCCT GAGGGCATCT TCCTTCGTGT ACCCCCTGGC 
AGCTATGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTGTTCGATA AAAGCCTCGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTCGGA 
AGCAACCCTA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTTTTAC CGAAGGCTAT GGTAGAGGTA GAAGATTTTG TGGATCCCAA TGGTAAGATC 
TCTCTACAGC GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTCCT TAGTTTTGTA 
AGCTGGCTGA CACTGAGTGG TCCTGAGCAG TCCAGTGTCC GGGGCCCATC GACAGAGAAC 
CAAGAGGCCA AGCGAGTAGC CTTGGACTGC ATCAAGCAAT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAATCT CTGCAGGAGC TCATGAAGGC TCTGGTCTCG 
GTGACACCAG ATGAAGAGAC ATATGATGAA GAAGATGCTG CTTTCTGTCT GGAGATGCTG 
CTAAGGATTG TGTTAGAAAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT 
CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG 
GTAGGGCTGC TACGCCTAGC CATTCGGCTA CTCCGAAGAG AAGAGATTAG TGGCCAGGTC 
CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC 
CAGGTCGCTT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT 
GATGACTGGG CCACTCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA 
GATGCTCTAC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CTGGAGCACA ATCCGACAGT 
GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTAGACC GAGGGTACAC TTCCGACTCA 
GAAGTCTACA CTGACCATGG CAGGCCTGGC AAGATGCACC GATCAGCCAC AGACGCTGAC 
ATGGCCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGCA 
GCAGGACTCA GCAGGCCAGG CCCTTCGCCC TTGGTCAATC AGTACAGCCT TACAGTGGGC 
CTGGACCTGG GACCACATGA CACTAAGTCC TTGCTTAAGT GTGTGGAGTC TCTGTCCTTC 
ATTGTTCGTG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACCCTC 
CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGTGGCAAG 
AGTCATAAAT ATGACAGCAA AGGGAACCGG TTCAAGAAAA AGCCCAAGGA GGGCTCGATG 
CTTCGGCGGC CCCGGGCATC CAGCCAGCAT GCCACTCGGG CTGGACACAG TGATGAAGAG 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
CTAGACCTGA TGCACACCCT GCACACACGG GCAGCCTCTA TCTACAGTTC ATGGGCAGAG 
GAGCAACGCC ACCTGGAGAC AGGTGGCCGG AAGATTGAAG CTGACTCACG TACCCTCTGG 
GCCCACTGCT GGTGCCCTTT ATTACAAGGT ATCGCTTGCT TATGCTGTGA CGCCCGGCGC 
CAGGTGCGGA TGCAGGCCCT GACATATCTC CAGCGAGCCC TTCTGGTACA TGACCTGCAA 
AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC GCTACTGACC 
AAGCTTTTAG AAAATATCAG CCCTGCGGAT GTGGGCGGGA TGGAGGAGAC CCGGATGCGG 
GCTTCCACAC TGCTCTCAAA GGTCTTCCTG CAGCACCTGT CCCCACTGCT GTCCCTGTCC 
ACCTTTGCTG CCCTGTGGCT CACCATCCTG GACTTCATGG ACAAGTACAT GCACGCAGGT 
TCCAGTGACT TGCTGTCAGA AGCTGTCCCT GAGTCTCTGA AGAACATGCT CCTTGTGATG 
GACACAGCAG AGATTTTCCA CAGTGCAGAT GCAAGGGGAG GCAGCCCCTC TGCTCTCTGG 
GAGATCACCT GGGAGCGCAT CGATTGCTTC TTACCACACC TACGTGATGA ACTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCCACA GAGCCTCACA GTCAAAAACC TCTGGCTTCT 
ACTCACCTGA CGCCTGCTGC TGGTGACCCC AGGATGCCTG GCCATCCACC TCCTCCAGAG 
ATACCCTCAG AAGTGGGAGC TTGTGACCCG GAGAAGCCCG AGAGCACCAG AGCTGCCAGC 
AGCAGCTCCC CTGGATCGCC GGTGGCCTCT AGCCCCAGCA AACAGAGTCC TGCCCCAGAG 
GGACCTCCCC CACTGGCCCA GCCCCCACTA ATCCTACAGC CCTTGACCTC CCCACTGCAG 
GTGGGGGTAC CACCCATGAC TTTGCCCATC ATCCTCAACC CTGCACTCAT TGAGGCCACC 
TCTCCAGTGC CTCTCTTAGC CACGCCACGC CCTACAGACC CCATCCCCAC CTCTGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231496.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231558.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X1, mRNA.

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CloneID	ORa24164
Clone ID Related Accession (Same CDS sequence)	XM_006231496.3
Accession Version	XM_006231496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG CAGGAAGGGG CCCATGTAGA AAAGGCCCAG 
TCAGCATCGG TGGAATCTAT CCCTGAAGTG CTAGAGGAGT GCACATCCCC TACTGACCAT 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TATGTCAATC CTCGGGGCGT GCGCTTTACC 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTTCCTTATG GTCTTCCTTG CATCCGTGAG 
CTCTTCCGCT TCCTTATCTC CCTCACAAAT CCACATGACC GCCATAACTC TGAGGTTATG 
ATCCACATGG GACTTCATTT GCTGACAGTA GCTCTTGAGT CAGCCCCTGT GGCCCAGTGC 
CAGACCCTCT TGGGTCTCAT CAAGGATGAG ATGTGCCGAC ACTTATTCCA GCTACTCAGC 
GTAGAGCGGC TGAACCTTTA CGCTGCTTCC CTACGAGTGT GCTTTTTGCT CTTTGAGAGC 
ATGCGGGAAC ACCTCAAGTT CCAGTTGGAG ATGTACATCA AAAAGCTCAT GGAGATTATC 
ACTGTTGAAA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCGCTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTCGTCACG GAGCTCTACA TCAACTATGA CTGTGACTAC 
TACTGCTCCA ACCTCTTCGA AGACCTCACT AAGCTGCTGT CCAAGAATGC CTTTCCTGTG 
TCTGGCCAGC TTTATACAAC ACACCTGCTG TCTCTTGATG CCCTATTGAC AGTTATTGAC 
AGCACTGAGG CTCATTGTCA GGCCAAAGTC CTCAACACCC TCAACCAGCA AGAGAAGAAA 
GAGACAGCCA GACCTGGCTT TGAGGCAGTG GATGGCAGCC CAGACACTTA CAAGTCTGAG 
AGAGCAGCCA GTGATGGGAA AGCCACAGGA GTGCCCTCAG ATGCTCCAGG CCTTCATTTT 
TCAAGTGGAG GGTGGTTGTC AACAGAACAT GGAAAGCCAG GGTGCAGTGA TCTGGAGGAA 
GCTGGTGACT CTGGGGTTGA CAAAAAGACC ACCAGGAAGC CACCTCGATT TTCCTGTCTT 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AAAAGCTGCT GATCACTGGT 
ACAGAGCAGT TCAACCAAAA ACCAAAGAAG GGGATCCAGT TTCTGCAGGA GAAAGGCCTC 
CTCACCATCC CAATGGATAA CACAGAGGTA GCCCAGTGGC TCCGAGAAAA CCCTCGGCTA 
GACAAAAAAA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATGGACCT GTTGGAGAGT 
TTTGTGAGCA CCTTCAGCTT CCAGGGTCTG AGGCTTGATG AGGCTCTCCG CCTCTACCTA 
GAAGCCTTCC GTCTGCCTGG GGAAGCGCCT GTTATTCACA GGCTGCTGGA GGTGTTCACT 
GAGCACTGGA GGAGTTGTAA TGGCTCCCCA TTTGCTAATA GCGATGCCTG CTTTGCCCTG 
GCCTATGCTG TTATCATGCT TAACACTGAC CAACATAACC ACAATGTTCG CAAGCAAAAT 
GCACCCATGA CCCTGGAGGA GTTCCGCAAA AACCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA AGACATGTAC CATGCCATCA AGAATGAGGA AATTGTGATG 
CCTGAGGAAC AAACAGGTCT GGTTCGTGAG AATTATGTGT GGAGTGTGCT GCTTCATCGA 
GGTGCTACCC CTGAGGGCAT CTTCCTTCGT GTACCCCCTG GCAGCTATGA TCTTGACCTC 
TTCACTATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTGTTCGA TAAAAGCCTC 
GAGGAGACCA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGTGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTGTGCAA ATTCACAGCT 
CTAAGTAGTG AGTCTATTGA GAACCTGCCC AGTGTATTCG GAAGCAACCC TAAAGCCCAC 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT TCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTTTT ACCGAAGGCT 
ATGGTAGAGG TAGAAGATTT TGTGGATCCC AATGGTAAGA TCTCTCTACA GCGGGAGGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTC CTTAGTTTTG TAAGCTGGCT GACACTGAGT 
GGTCCTGAGC AGTCCAGTGT CCGGGGCCCA TCGACAGAGA ACCAAGAGGC CAAGCGAGTA 
GCCTTGGACT GCATCAAGCA ATGTGACCCA GAAAAGATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAAT CTCTGCAGGA GCTCATGAAG GCTCTGGTCT CGGTGACACC AGATGAAGAG 
ACATATGATG AAGAAGATGC TGCTTTCTGT CTGGAGATGC TGCTAAGGAT TGTGTTAGAA 
AACAGGGACC GTGTGGGCTG TGTATGGCAG ACTGTTCGAG ATCATCTATA CCACCTGTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGAGCAG TGGTAGGGCT GCTACGCCTA 
GCCATTCGGC TACTCCGAAG AGAAGAGATT AGTGGCCAGG TCCTGCTCTC TCTGCGCATC 
TTGTTACTGA TGAAGCCCAG CGTGCTGTCC AGAGTCAGCC ACCAGGTCGC TTATGGGCTG 
CATGAACTCC TCAAGACCAA TGCTGCCAAC ATCCACTCGG GTGATGACTG GGCCACTCTC 
TTCACATTGC TGGAGTGCAT TGGCTCAGGC GTGAAGCCTC CAGATGCTCT ACAAGCCACA 
GCCAGGGCTG ATGCTCCTGA TGCTGGAGCA CAATCCGACA GTGAGCTCCC ATCCTACCAT 
CAAAATGATG TCAGCCTAGA CCGAGGGTAC ACTTCCGACT CAGAAGTCTA CACTGACCAT 
GGCAGGCCTG GCAAGATGCA CCGATCAGCC ACAGACGCTG ACATGGCCAA CAGTGGTTGG 
TTAGTGGTGG GGAAGGATGA CATTGATAAC TCCAAACCAG CAGCAGGACT CAGCAGGCCA 
GGCCCTTCGC CCTTGGTCAA TCAGTACAGC CTTACAGTGG GCCTGGACCT GGGACCACAT 
GACACTAAGT CCTTGCTTAA GTGTGTGGAG TCTCTGTCCT TCATTGTTCG TGATGCTGCT 
CACATCACCC CTGACAACTT TGAACTCTGC GTCAAGACCC TCCGCATCTT TGTAGAAGCC 
AGTCTGAATG GTGGGTGCAA GTCCCAGGAT AAACGTGGCA AGAGTCATAA ATATGACAGC 
AAAGGGAACC GGTTCAAGAA AAAGCCCAAG GAGGGCTCGA TGCTTCGGCG GCCCCGGGCA 
TCCAGCCAGC ATGCCACTCG GGCTGGACAC AGTGATGAAG AGGAGGACGA AGGCGTGCCT 
GCCAGCTACC ATACGGTGTC TTTACAGGTC AGTCAGGACT TGCTAGACCT GATGCACACC 
CTGCACACAC GGGCAGCCTC TATCTACAGT TCATGGGCAG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC GGAAGATTGA AGCTGACTCA CGTACCCTCT GGGCCCACTG CTGGTGCCCT 
TTATTACAAG GTATCGCTTG CTTATGCTGT GACGCCCGGC GCCAGGTGCG GATGCAGGCC 
CTGACATATC TCCAGCGAGC CCTTCTGGTA CATGACCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCGCTACTGA CCAAGCTTTT AGAAAATATC 
AGCCCTGCGG ATGTGGGCGG GATGGAGGAG ACCCGGATGC GGGCTTCCAC ACTGCTCTCA 
AAGGTCTTCC TGCAGCACCT GTCCCCACTG CTGTCCCTGT CCACCTTTGC TGCCCTGTGG 
CTCACCATCC TGGACTTCAT GGACAAGTAC ATGCACGCAG GTTCCAGTGA CTTGCTGTCA 
GAAGCTGTCC CTGAGTCTCT GAAGAACATG CTCCTTGTGA TGGACACAGC AGAGATTTTC 
CACAGTGCAG ATGCAAGGGG AGGCAGCCCC TCTGCTCTCT GGGAGATCAC CTGGGAGCGC 
ATCGATTGCT TCTTACCACA CCTACGTGAT GAACTCTTCA AGCAGACTGT CATCCAGGAC 
CCCATGCCCA CAGAGCCTCA CAGTCAAAAA CCTCTGGCTT CTACTCACCT GACGCCTGCT 
GCTGGTGACC CCAGGATGCC TGGCCATCCA CCTCCTCCAG AGATACCCTC AGAAGTGGGA 
GCTTGTGACC CGGAGAAGCC CGAGAGCACC AGAGCTGCCA GCAGCAGCTC CCCTGGATCG 
CCGGTGGCCT CTAGCCCCAG CAAACAGAGT CCTGCCCCAG AGGGACCTCC CCCACTGGCC 
CAGCCCCCAC TAATCCTACA GCCCTTGACC TCCCCACTGC AGGTGGGGGT ACCACCCATG 
ACTTTGCCCA TCATCCTCAA CCCTGCACTC ATTGAGGCCA CCTCTCCAGT GCCTCTCTTA 
GCCACGCCAC GCCCTACAGA CCCCATCCCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231558.1
CDS	278..5965
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKISPCLLNTLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231496.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231496.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG CAGGAAGGGG CCCATGTAGA AAAGGCCCAG 
TCAGCATCGG TGGAATCTAT CCCTGAAGTG CTAGAGGAGT GCACATCCCC TACTGACCAT 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TATGTCAATC CTCGGGGCGT GCGCTTTACC 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTTCCTTATG GTCTTCCTTG CATCCGTGAG 
CTCTTCCGCT TCCTTATCTC CCTCACAAAT CCACATGACC GCCATAACTC TGAGGTTATG 
ATCCACATGG GACTTCATTT GCTGACAGTA GCTCTTGAGT CAGCCCCTGT GGCCCAGTGC 
CAGACCCTCT TGGGTCTCAT CAAGGATGAG ATGTGCCGAC ACTTATTCCA GCTACTCAGC 
GTAGAGCGGC TGAACCTTTA CGCTGCTTCC CTACGAGTGT GCTTTTTGCT CTTTGAGAGC 
ATGCGGGAAC ACCTCAAGTT CCAGTTGGAG ATGTACATCA AAAAGCTCAT GGAGATTATC 
ACTGTTGAAA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCGCTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTCGTCACG GAGCTCTACA TCAACTATGA CTGTGACTAC 
TACTGCTCCA ACCTCTTCGA AGACCTCACT AAGCTGCTGT CCAAGAATGC CTTTCCTGTG 
TCTGGCCAGC TTTATACAAC ACACCTGCTG TCTCTTGATG CCCTATTGAC AGTTATTGAC 
AGCACTGAGG CTCATTGTCA GGCCAAAGTC CTCAACACCC TCAACCAGCA AGAGAAGAAA 
GAGACAGCCA GACCTGGCTT TGAGGCAGTG GATGGCAGCC CAGACACTTA CAAGTCTGAG 
AGAGCAGCCA GTGATGGGAA AGCCACAGGA GTGCCCTCAG ATGCTCCAGG CCTTCATTTT 
TCAAGTGGAG GGTGGTTGTC AACAGAACAT GGAAAGCCAG GGTGCAGTGA TCTGGAGGAA 
GCTGGTGACT CTGGGGTTGA CAAAAAGACC ACCAGGAAGC CACCTCGATT TTCCTGTCTT 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AAAAGCTGCT GATCACTGGT 
ACAGAGCAGT TCAACCAAAA ACCAAAGAAG GGGATCCAGT TTCTGCAGGA GAAAGGCCTC 
CTCACCATCC CAATGGATAA CACAGAGGTA GCCCAGTGGC TCCGAGAAAA CCCTCGGCTA 
GACAAAAAAA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATGGACCT GTTGGAGAGT 
TTTGTGAGCA CCTTCAGCTT CCAGGGTCTG AGGCTTGATG AGGCTCTCCG CCTCTACCTA 
GAAGCCTTCC GTCTGCCTGG GGAAGCGCCT GTTATTCACA GGCTGCTGGA GGTGTTCACT 
GAGCACTGGA GGAGTTGTAA TGGCTCCCCA TTTGCTAATA GCGATGCCTG CTTTGCCCTG 
GCCTATGCTG TTATCATGCT TAACACTGAC CAACATAACC ACAATGTTCG CAAGCAAAAT 
GCACCCATGA CCCTGGAGGA GTTCCGCAAA AACCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA AGACATGTAC CATGCCATCA AGAATGAGGA AATTGTGATG 
CCTGAGGAAC AAACAGGTCT GGTTCGTGAG AATTATGTGT GGAGTGTGCT GCTTCATCGA 
GGTGCTACCC CTGAGGGCAT CTTCCTTCGT GTACCCCCTG GCAGCTATGA TCTTGACCTC 
TTCACTATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTGTTCGA TAAAAGCCTC 
GAGGAGACCA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGTGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTGTGCAA ATTCACAGCT 
CTAAGTAGTG AGTCTATTGA GAACCTGCCC AGTGTATTCG GAAGCAACCC TAAAGCCCAC 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT TCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTTTT ACCGAAGGCT 
ATGGTAGAGG TAGAAGATTT TGTGGATCCC AATGGTAAGA TCTCTCTACA GCGGGAGGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTC CTTAGTTTTG TAAGCTGGCT GACACTGAGT 
GGTCCTGAGC AGTCCAGTGT CCGGGGCCCA TCGACAGAGA ACCAAGAGGC CAAGCGAGTA 
GCCTTGGACT GCATCAAGCA ATGTGACCCA GAAAAGATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAAT CTCTGCAGGA GCTCATGAAG GCTCTGGTCT CGGTGACACC AGATGAAGAG 
ACATATGATG AAGAAGATGC TGCTTTCTGT CTGGAGATGC TGCTAAGGAT TGTGTTAGAA 
AACAGGGACC GTGTGGGCTG TGTATGGCAG ACTGTTCGAG ATCATCTATA CCACCTGTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGAGCAG TGGTAGGGCT GCTACGCCTA 
GCCATTCGGC TACTCCGAAG AGAAGAGATT AGTGGCCAGG TCCTGCTCTC TCTGCGCATC 
TTGTTACTGA TGAAGCCCAG CGTGCTGTCC AGAGTCAGCC ACCAGGTCGC TTATGGGCTG 
CATGAACTCC TCAAGACCAA TGCTGCCAAC ATCCACTCGG GTGATGACTG GGCCACTCTC 
TTCACATTGC TGGAGTGCAT TGGCTCAGGC GTGAAGCCTC CAGATGCTCT ACAAGCCACA 
GCCAGGGCTG ATGCTCCTGA TGCTGGAGCA CAATCCGACA GTGAGCTCCC ATCCTACCAT 
CAAAATGATG TCAGCCTAGA CCGAGGGTAC ACTTCCGACT CAGAAGTCTA CACTGACCAT 
GGCAGGCCTG GCAAGATGCA CCGATCAGCC ACAGACGCTG ACATGGCCAA CAGTGGTTGG 
TTAGTGGTGG GGAAGGATGA CATTGATAAC TCCAAACCAG CAGCAGGACT CAGCAGGCCA 
GGCCCTTCGC CCTTGGTCAA TCAGTACAGC CTTACAGTGG GCCTGGACCT GGGACCACAT 
GACACTAAGT CCTTGCTTAA GTGTGTGGAG TCTCTGTCCT TCATTGTTCG TGATGCTGCT 
CACATCACCC CTGACAACTT TGAACTCTGC GTCAAGACCC TCCGCATCTT TGTAGAAGCC 
AGTCTGAATG GTGGGTGCAA GTCCCAGGAT AAACGTGGCA AGAGTCATAA ATATGACAGC 
AAAGGGAACC GGTTCAAGAA AAAGCCCAAG GAGGGCTCGA TGCTTCGGCG GCCCCGGGCA 
TCCAGCCAGC ATGCCACTCG GGCTGGACAC AGTGATGAAG AGGAGGACGA AGGCGTGCCT 
GCCAGCTACC ATACGGTGTC TTTACAGGTC AGTCAGGACT TGCTAGACCT GATGCACACC 
CTGCACACAC GGGCAGCCTC TATCTACAGT TCATGGGCAG AGGAGCAACG CCACCTGGAG 
ACAGGTGGCC GGAAGATTGA AGCTGACTCA CGTACCCTCT GGGCCCACTG CTGGTGCCCT 
TTATTACAAG GTATCGCTTG CTTATGCTGT GACGCCCGGC GCCAGGTGCG GATGCAGGCC 
CTGACATATC TCCAGCGAGC CCTTCTGGTA CATGACCTGC AAAAGCTAGA TGCCCTGGAA 
TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCGCTACTGA CCAAGCTTTT AGAAAATATC 
AGCCCTGCGG ATGTGGGCGG GATGGAGGAG ACCCGGATGC GGGCTTCCAC ACTGCTCTCA 
AAGGTCTTCC TGCAGCACCT GTCCCCACTG CTGTCCCTGT CCACCTTTGC TGCCCTGTGG 
CTCACCATCC TGGACTTCAT GGACAAGTAC ATGCACGCAG GTTCCAGTGA CTTGCTGTCA 
GAAGCTGTCC CTGAGTCTCT GAAGAACATG CTCCTTGTGA TGGACACAGC AGAGATTTTC 
CACAGTGCAG ATGCAAGGGG AGGCAGCCCC TCTGCTCTCT GGGAGATCAC CTGGGAGCGC 
ATCGATTGCT TCTTACCACA CCTACGTGAT GAACTCTTCA AGCAGACTGT CATCCAGGAC 
CCCATGCCCA CAGAGCCTCA CAGTCAAAAA CCTCTGGCTT CTACTCACCT GACGCCTGCT 
GCTGGTGACC CCAGGATGCC TGGCCATCCA CCTCCTCCAG AGATACCCTC AGAAGTGGGA 
GCTTGTGACC CGGAGAAGCC CGAGAGCACC AGAGCTGCCA GCAGCAGCTC CCCTGGATCG 
CCGGTGGCCT CTAGCCCCAG CAAACAGAGT CCTGCCCCAG AGGGACCTCC CCCACTGGCC 
CAGCCCCCAC TAATCCTACA GCCCTTGACC TCCCCACTGC AGGTGGGGGT ACCACCCATG 
ACTTTGCCCA TCATCCTCAA CCCTGCACTC ATTGAGGCCA CCTCTCCAGT GCCTCTCTTA 
GCCACGCCAC GCCCTACAGA CCCCATCCCC ACCTCTGAGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231497.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231559.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X2, mRNA.

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CloneID	ORa24165
Clone ID Related Accession (Same CDS sequence)	XM_006231497.3
Accession Version	XM_006231497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG GAAGGGGCCC ATGTAGAAAA GGCCCAGTCA 
GCATCGGTGG AATCTATCCC TGAAGTGCTA GAGGAGTGCA CATCCCCTAC TGACCATTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAT GTCAATCCTC GGGGCGTGCG CTTTACCCAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTT CCTTATGGTC TTCCTTGCAT CCGTGAGCTC 
TTCCGCTTCC TTATCTCCCT CACAAATCCA CATGACCGCC ATAACTCTGA GGTTATGATC 
CACATGGGAC TTCATTTGCT GACAGTAGCT CTTGAGTCAG CCCCTGTGGC CCAGTGCCAG 
ACCCTCTTGG GTCTCATCAA GGATGAGATG TGCCGACACT TATTCCAGCT ACTCAGCGTA 
GAGCGGCTGA ACCTTTACGC TGCTTCCCTA CGAGTGTGCT TTTTGCTCTT TGAGAGCATG 
CGGGAACACC TCAAGTTCCA GTTGGAGATG TACATCAAAA AGCTCATGGA GATTATCACT 
GTTGAAAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CGCTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT CGTCACGGAG CTCTACATCA ACTATGACTG TGACTACTAC 
TGCTCCAACC TCTTCGAAGA CCTCACTAAG CTGCTGTCCA AGAATGCCTT TCCTGTGTCT 
GGCCAGCTTT ATACAACACA CCTGCTGTCT CTTGATGCCC TATTGACAGT TATTGACAGC 
ACTGAGGCTC ATTGTCAGGC CAAAGTCCTC AACACCCTCA ACCAGCAAGA GAAGAAAGAG 
ACAGCCAGAC CTGGCTTTGA GGCAGTGGAT GGCAGCCCAG ACACTTACAA GTCTGAGAGA 
GCAGCCAGTG ATGGGAAAGC CACAGGAGTG CCCTCAGATG CTCCAGGCCT TCATTTTTCA 
AGTGGAGGGT GGTTGTCAAC AGAACATGGA AAGCCAGGGT GCAGTGATCT GGAGGAAGCT 
GGTGACTCTG GGGTTGACAA AAAGACCACC AGGAAGCCAC CTCGATTTTC CTGTCTTCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAAA AGCTGCTGAT CACTGGTACA 
GAGCAGTTCA ACCAAAAACC AAAGAAGGGG ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGATAACAC AGAGGTAGCC CAGTGGCTCC GAGAAAACCC TCGGCTAGAC 
AAAAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TGGACCTGTT GGAGAGTTTT 
GTGAGCACCT TCAGCTTCCA GGGTCTGAGG CTTGATGAGG CTCTCCGCCT CTACCTAGAA 
GCCTTCCGTC TGCCTGGGGA AGCGCCTGTT ATTCACAGGC TGCTGGAGGT GTTCACTGAG 
CACTGGAGGA GTTGTAATGG CTCCCCATTT GCTAATAGCG ATGCCTGCTT TGCCCTGGCC 
TATGCTGTTA TCATGCTTAA CACTGACCAA CATAACCACA ATGTTCGCAA GCAAAATGCA 
CCCATGACCC TGGAGGAGTT CCGCAAAAAC CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAAGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAGGAACAAA CAGGTCTGGT TCGTGAGAAT TATGTGTGGA GTGTGCTGCT TCATCGAGGT 
GCTACCCCTG AGGGCATCTT CCTTCGTGTA CCCCCTGGCA GCTATGATCT TGACCTCTTC 
ACTATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TGTTCGATAA AAGCCTCGAG 
GAGACCATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GTGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TGTGCAAATT CACAGCTCTA 
AGTAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTCGGAA GCAACCCTAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTTCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTTTTACC GAAGGCTATG 
GTAGAGGTAG AAGATTTTGT GGATCCCAAT GGTAAGATCT CTCTACAGCG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTCCTT AGTTTTGTAA GCTGGCTGAC ACTGAGTGGT 
CCTGAGCAGT CCAGTGTCCG GGGCCCATCG ACAGAGAACC AAGAGGCCAA GCGAGTAGCC 
TTGGACTGCA TCAAGCAATG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAATCTC TGCAGGAGCT CATGAAGGCT CTGGTCTCGG TGACACCAGA TGAAGAGACA 
TATGATGAAG AAGATGCTGC TTTCTGTCTG GAGATGCTGC TAAGGATTGT GTTAGAAAAC 
AGGGACCGTG TGGGCTGTGT ATGGCAGACT GTTCGAGATC ATCTATACCA CCTGTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGAGCAGTGG TAGGGCTGCT ACGCCTAGCC 
ATTCGGCTAC TCCGAAGAGA AGAGATTAGT GGCCAGGTCC TGCTCTCTCT GCGCATCTTG 
TTACTGATGA AGCCCAGCGT GCTGTCCAGA GTCAGCCACC AGGTCGCTTA TGGGCTGCAT 
GAACTCCTCA AGACCAATGC TGCCAACATC CACTCGGGTG ATGACTGGGC CACTCTCTTC 
ACATTGCTGG AGTGCATTGG CTCAGGCGTG AAGCCTCCAG ATGCTCTACA AGCCACAGCC 
AGGGCTGATG CTCCTGATGC TGGAGCACAA TCCGACAGTG AGCTCCCATC CTACCATCAA 
AATGATGTCA GCCTAGACCG AGGGTACACT TCCGACTCAG AAGTCTACAC TGACCATGGC 
AGGCCTGGCA AGATGCACCG ATCAGCCACA GACGCTGACA TGGCCAACAG TGGTTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAACCAGCAG CAGGACTCAG CAGGCCAGGC 
CCTTCGCCCT TGGTCAATCA GTACAGCCTT ACAGTGGGCC TGGACCTGGG ACCACATGAC 
ACTAAGTCCT TGCTTAAGTG TGTGGAGTCT CTGTCCTTCA TTGTTCGTGA TGCTGCTCAC 
ATCACCCCTG ACAACTTTGA ACTCTGCGTC AAGACCCTCC GCATCTTTGT AGAAGCCAGT 
CTGAATGGTG GGTGCAAGTC CCAGGATAAA CGTGGCAAGA GTCATAAATA TGACAGCAAA 
GGGAACCGGT TCAAGAAAAA GCCCAAGGAG GGCTCGATGC TTCGGCGGCC CCGGGCATCC 
AGCCAGCATG CCACTCGGGC TGGACACAGT GATGAAGAGG AGGACGAAGG CGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGC TAGACCTGAT GCACACCCTG 
CACACACGGG CAGCCTCTAT CTACAGTTCA TGGGCAGAGG AGCAACGCCA CCTGGAGACA 
GGTGGCCGGA AGATTGAAGC TGACTCACGT ACCCTCTGGG CCCACTGCTG GTGCCCTTTA 
TTACAAGGTA TCGCTTGCTT ATGCTGTGAC GCCCGGCGCC AGGTGCGGAT GCAGGCCCTG 
ACATATCTCC AGCGAGCCCT TCTGGTACAT GACCTGCAAA AGCTAGATGC CCTGGAATGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCG CTACTGACCA AGCTTTTAGA AAATATCAGC 
CCTGCGGATG TGGGCGGGAT GGAGGAGACC CGGATGCGGG CTTCCACACT GCTCTCAAAG 
GTCTTCCTGC AGCACCTGTC CCCACTGCTG TCCCTGTCCA CCTTTGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCAGGTT CCAGTGACTT GCTGTCAGAA 
GCTGTCCCTG AGTCTCTGAA GAACATGCTC CTTGTGATGG ACACAGCAGA GATTTTCCAC 
AGTGCAGATG CAAGGGGAGG CAGCCCCTCT GCTCTCTGGG AGATCACCTG GGAGCGCATC 
GATTGCTTCT TACCACACCT ACGTGATGAA CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCCACAG AGCCTCACAG TCAAAAACCT CTGGCTTCTA CTCACCTGAC GCCTGCTGCT 
GGTGACCCCA GGATGCCTGG CCATCCACCT CCTCCAGAGA TACCCTCAGA AGTGGGAGCT 
TGTGACCCGG AGAAGCCCGA GAGCACCAGA GCTGCCAGCA GCAGCTCCCC TGGATCGCCG 
GTGGCCTCTA GCCCCAGCAA ACAGAGTCCT GCCCCAGAGG GACCTCCCCC ACTGGCCCAG 
CCCCCACTAA TCCTACAGCC CTTGACCTCC CCACTGCAGG TGGGGGTACC ACCCATGACT 
TTGCCCATCA TCCTCAACCC TGCACTCATT GAGGCCACCT CTCCAGTGCC TCTCTTAGCC 
ACGCCACGCC CTACAGACCC CATCCCCACC TCTGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231559.1
CDS	278..5962
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKISPCLLNTLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231497.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231497.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG GAAGGGGCCC ATGTAGAAAA GGCCCAGTCA 
GCATCGGTGG AATCTATCCC TGAAGTGCTA GAGGAGTGCA CATCCCCTAC TGACCATTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAT GTCAATCCTC GGGGCGTGCG CTTTACCCAG 
TCCTCCCAGA AAGAAGGCAC AGCTTTGGTT CCTTATGGTC TTCCTTGCAT CCGTGAGCTC 
TTCCGCTTCC TTATCTCCCT CACAAATCCA CATGACCGCC ATAACTCTGA GGTTATGATC 
CACATGGGAC TTCATTTGCT GACAGTAGCT CTTGAGTCAG CCCCTGTGGC CCAGTGCCAG 
ACCCTCTTGG GTCTCATCAA GGATGAGATG TGCCGACACT TATTCCAGCT ACTCAGCGTA 
GAGCGGCTGA ACCTTTACGC TGCTTCCCTA CGAGTGTGCT TTTTGCTCTT TGAGAGCATG 
CGGGAACACC TCAAGTTCCA GTTGGAGATG TACATCAAAA AGCTCATGGA GATTATCACT 
GTTGAAAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CGCTGGAGGC CATTGTGCAG 
CTCTGGCGCA TCCCCAGCTT CGTCACGGAG CTCTACATCA ACTATGACTG TGACTACTAC 
TGCTCCAACC TCTTCGAAGA CCTCACTAAG CTGCTGTCCA AGAATGCCTT TCCTGTGTCT 
GGCCAGCTTT ATACAACACA CCTGCTGTCT CTTGATGCCC TATTGACAGT TATTGACAGC 
ACTGAGGCTC ATTGTCAGGC CAAAGTCCTC AACACCCTCA ACCAGCAAGA GAAGAAAGAG 
ACAGCCAGAC CTGGCTTTGA GGCAGTGGAT GGCAGCCCAG ACACTTACAA GTCTGAGAGA 
GCAGCCAGTG ATGGGAAAGC CACAGGAGTG CCCTCAGATG CTCCAGGCCT TCATTTTTCA 
AGTGGAGGGT GGTTGTCAAC AGAACATGGA AAGCCAGGGT GCAGTGATCT GGAGGAAGCT 
GGTGACTCTG GGGTTGACAA AAAGACCACC AGGAAGCCAC CTCGATTTTC CTGTCTTCTG 
CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAAA AGCTGCTGAT CACTGGTACA 
GAGCAGTTCA ACCAAAAACC AAAGAAGGGG ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGATAACAC AGAGGTAGCC CAGTGGCTCC GAGAAAACCC TCGGCTAGAC 
AAAAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TGGACCTGTT GGAGAGTTTT 
GTGAGCACCT TCAGCTTCCA GGGTCTGAGG CTTGATGAGG CTCTCCGCCT CTACCTAGAA 
GCCTTCCGTC TGCCTGGGGA AGCGCCTGTT ATTCACAGGC TGCTGGAGGT GTTCACTGAG 
CACTGGAGGA GTTGTAATGG CTCCCCATTT GCTAATAGCG ATGCCTGCTT TGCCCTGGCC 
TATGCTGTTA TCATGCTTAA CACTGACCAA CATAACCACA ATGTTCGCAA GCAAAATGCA 
CCCATGACCC TGGAGGAGTT CCGCAAAAAC CTGAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAAGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAGGAACAAA CAGGTCTGGT TCGTGAGAAT TATGTGTGGA GTGTGCTGCT TCATCGAGGT 
GCTACCCCTG AGGGCATCTT CCTTCGTGTA CCCCCTGGCA GCTATGATCT TGACCTCTTC 
ACTATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TGTTCGATAA AAGCCTCGAG 
GAGACCATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GTGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TGTGCAAATT CACAGCTCTA 
AGTAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTCGGAA GCAACCCTAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTTCG GGAGGGCTGG 
AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTTTTACC GAAGGCTATG 
GTAGAGGTAG AAGATTTTGT GGATCCCAAT GGTAAGATCT CTCTACAGCG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC AACAGTCCTT AGTTTTGTAA GCTGGCTGAC ACTGAGTGGT 
CCTGAGCAGT CCAGTGTCCG GGGCCCATCG ACAGAGAACC AAGAGGCCAA GCGAGTAGCC 
TTGGACTGCA TCAAGCAATG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTCCAG 
CTGGAATCTC TGCAGGAGCT CATGAAGGCT CTGGTCTCGG TGACACCAGA TGAAGAGACA 
TATGATGAAG AAGATGCTGC TTTCTGTCTG GAGATGCTGC TAAGGATTGT GTTAGAAAAC 
AGGGACCGTG TGGGCTGTGT ATGGCAGACT GTTCGAGATC ATCTATACCA CCTGTGTGTT 
CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGAGCAGTGG TAGGGCTGCT ACGCCTAGCC 
ATTCGGCTAC TCCGAAGAGA AGAGATTAGT GGCCAGGTCC TGCTCTCTCT GCGCATCTTG 
TTACTGATGA AGCCCAGCGT GCTGTCCAGA GTCAGCCACC AGGTCGCTTA TGGGCTGCAT 
GAACTCCTCA AGACCAATGC TGCCAACATC CACTCGGGTG ATGACTGGGC CACTCTCTTC 
ACATTGCTGG AGTGCATTGG CTCAGGCGTG AAGCCTCCAG ATGCTCTACA AGCCACAGCC 
AGGGCTGATG CTCCTGATGC TGGAGCACAA TCCGACAGTG AGCTCCCATC CTACCATCAA 
AATGATGTCA GCCTAGACCG AGGGTACACT TCCGACTCAG AAGTCTACAC TGACCATGGC 
AGGCCTGGCA AGATGCACCG ATCAGCCACA GACGCTGACA TGGCCAACAG TGGTTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAACCAGCAG CAGGACTCAG CAGGCCAGGC 
CCTTCGCCCT TGGTCAATCA GTACAGCCTT ACAGTGGGCC TGGACCTGGG ACCACATGAC 
ACTAAGTCCT TGCTTAAGTG TGTGGAGTCT CTGTCCTTCA TTGTTCGTGA TGCTGCTCAC 
ATCACCCCTG ACAACTTTGA ACTCTGCGTC AAGACCCTCC GCATCTTTGT AGAAGCCAGT 
CTGAATGGTG GGTGCAAGTC CCAGGATAAA CGTGGCAAGA GTCATAAATA TGACAGCAAA 
GGGAACCGGT TCAAGAAAAA GCCCAAGGAG GGCTCGATGC TTCGGCGGCC CCGGGCATCC 
AGCCAGCATG CCACTCGGGC TGGACACAGT GATGAAGAGG AGGACGAAGG CGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGC TAGACCTGAT GCACACCCTG 
CACACACGGG CAGCCTCTAT CTACAGTTCA TGGGCAGAGG AGCAACGCCA CCTGGAGACA 
GGTGGCCGGA AGATTGAAGC TGACTCACGT ACCCTCTGGG CCCACTGCTG GTGCCCTTTA 
TTACAAGGTA TCGCTTGCTT ATGCTGTGAC GCCCGGCGCC AGGTGCGGAT GCAGGCCCTG 
ACATATCTCC AGCGAGCCCT TCTGGTACAT GACCTGCAAA AGCTAGATGC CCTGGAATGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCG CTACTGACCA AGCTTTTAGA AAATATCAGC 
CCTGCGGATG TGGGCGGGAT GGAGGAGACC CGGATGCGGG CTTCCACACT GCTCTCAAAG 
GTCTTCCTGC AGCACCTGTC CCCACTGCTG TCCCTGTCCA CCTTTGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCAGGTT CCAGTGACTT GCTGTCAGAA 
GCTGTCCCTG AGTCTCTGAA GAACATGCTC CTTGTGATGG ACACAGCAGA GATTTTCCAC 
AGTGCAGATG CAAGGGGAGG CAGCCCCTCT GCTCTCTGGG AGATCACCTG GGAGCGCATC 
GATTGCTTCT TACCACACCT ACGTGATGAA CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCCACAG AGCCTCACAG TCAAAAACCT CTGGCTTCTA CTCACCTGAC GCCTGCTGCT 
GGTGACCCCA GGATGCCTGG CCATCCACCT CCTCCAGAGA TACCCTCAGA AGTGGGAGCT 
TGTGACCCGG AGAAGCCCGA GAGCACCAGA GCTGCCAGCA GCAGCTCCCC TGGATCGCCG 
GTGGCCTCTA GCCCCAGCAA ACAGAGTCCT GCCCCAGAGG GACCTCCCCC ACTGGCCCAG 
CCCCCACTAA TCCTACAGCC CTTGACCTCC CCACTGCAGG TGGGGGTACC ACCCATGACT 
TTGCCCATCA TCCTCAACCC TGCACTCATT GAGGCCACCT CTCCAGTGCC TCTCTTAGCC 
ACGCCACGCC CTACAGACCC CATCCCCACC TCTGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231498.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231560.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24166
Clone ID Related Accession (Same CDS sequence)	XM_006231498.3
Accession Version	XM_006231498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG CAGGAAGGGG CCCATGTAGA AAAGGCCCAG 
TCAGCATCGG TGGAATCTAT CCCTGAAGTG CTAGAGGAGT GCACATCCCC TACTGACCAT 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TATGTCAATC CTCGGGGCGT GCGCTTTACC 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTTCCTTATG GTCTTCCTTG CATCCGTGAG 
CTCTTCCGCT TCCTTATCTC CCTCACAAAT CCACATGACC GCCATAACTC TGAGGTTATG 
ATCCACATGG GACTTCATTT GCTGACAGTA GCTCTTGAGT CAGCCCCTGT GGCCCAGTGC 
CAGACCCTCT TGGGTCTCAT CAAGGATGAG ATGTGCCGAC ACTTATTCCA GCTACTCAGC 
GTAGAGCGGC TGAACCTTTA CGCTGCTTCC CTACGAGTGT GCTTTTTGCT CTTTGAGAGC 
ATGCGGGAAC ACCTCAAGTT CCAGTTGGAG ATGTACATCA AAAAGCTCAT GGAGATTATC 
ACTGTTGAAA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCGCTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTCGTCACG GAGCTCTACA TCAACTATGA CTGTGACTAC 
TACTGCTCCA ACCTCTTCGA AGACCTCACT AAGCTGCTGT CCAAGAATGC CTTTCCTGTG 
TCTGGCCAGC TTTATACAAC ACACCTGCTG TCTCTTGATG CCCTATTGAC AGTTATTGAC 
AGCACTGAGG CTCATTGTCA GGCCAAAGTC CTCAACACCC TCAACCAGCA AGAGAAGAAA 
GAGACAGCCA GACCTGGCTT TGAGGCAGTG GATGGCAGCC CAGACACTTA CAAGTCTGAG 
AGAGCAGCCA GTGATGGGAA AGCCACAGGA GTGCCCTCAG ATGCTCCAGG CCTTCATTTT 
TCAAGTGGAG GGTGGTTGTC AACAGAACAT GGAAAGCCAG GGTGCAGTGA TCTGGAGGAA 
GCTGGTGACT CTGGGGTTGA CAAAAAGACC ACCAGGAAGC CACCTCGATT TTCCTGTCTT 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AAAAGCTGCT GATCACTGGT 
ACAGAGCAGT TCAACCAAAA ACCAAAGAAG GGGATCCAGT TTCTGCAGGA GAAAGGCCTC 
CTCACCATCC CAATGGATAA CACAGAGGTA GCCCAGTGGC TCCGAGAAAA CCCTCGGCTA 
GACAAAAAAA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATGGACCT GTTGGAGAGT 
TTTGTGAGCA CCTTCAGCTT CCAGGGTCTG AGGCTTGATG AGGCTCTCCG CCTCTACCTA 
GAAGCCTTCC GTCTGCCTGG GGAAGCGCCT GTTATTCACA GGCTGCTGGA GGTGTTCACT 
GAGCACTGGA GGAGTTGTAA TGGCTCCCCA TTTGCTAATA GCGATGCCTG CTTTGCCCTG 
GCCTATGCTG TTATCATGCT TAACACTGAC CAACATAACC ACAATGTTCG CAAGCAAAAT 
GCACCCATGA CCCTGGAGGA GTTCCGCAAA AACCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA AGACATGTAC CATGCCATCA AGAATGAGGA AATTGTGATG 
CCTGAGGAAC AAACAGGTCT GGTTCGTGAG AATTATGTGT GGAGTGTGCT GCTTCATCGA 
GGTGCTACCC CTGAGGGCAT CTTCCTTCGT GTACCCCCTG GCAGCTATGA TCTTGACCTC 
TTCACTATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTGTTCGA TAAAAGCCTC 
GAGGAGACCA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGTGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTGTGCAA ATTCACAGCT 
CTAAGTAGTG AGTCTATTGA GAACCTGCCC AGTGTATTCG GAAGCAACCC TAAAGCCCAC 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT TCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTTTT ACCGAAGGCT 
ATGGTAGAGG TAGAAGATTT TGTGGATCCC AATGGTAAGA TCTCTCTACA GCGGGAGGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTC CTTAGTTTTG TAAGCTGGCT GACACTGAGT 
GGTCCTGAGC AGTCCAGTGT CCGGGGCCCA TCGACAGAGA ACCAAGAGGC CAAGCGAGTA 
GCCTTGGACT GCATCAAGCA ATGTGACCCA GAAAAGATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAAT CTCTGCAGGA GCTCATGAAG GCTCTGGTCT CGGTGACACC AGATGAAGAG 
ACATATGATG AAGAAGATGC TGCTTTCTGT CTGGAGATGC TGCTAAGGAT TGTGTTAGAA 
AACAGGGACC GTGTGGGCTG TGTATGGCAG ACTGTTCGAG ATCATCTATA CCACCTGTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGAGCAG TGGTAGGGCT GCTACGCCTA 
GCCATTCGGC TACTCCGAAG AGAAGAGATT AGTGGCCAGG TCCTGCTCTC TCTGCGCATC 
TTGTTACTGA TGAAGCCCAG CGTGCTGTCC AGAGTCAGCC ACCAGGTCGC TTATGGGCTG 
CATGAACTCC TCAAGACCAA TGCTGCCAAC ATCCACTCGG GTGATGACTG GGCCACTCTC 
TTCACATTGC TGGAGTGCAT TGGCTCAGGC GTGAAGCCTC CAGATGCTCT ACAAGCCACA 
GCCAGGGCTG ATGCTCCTGA TGCTGGAGCA CAATCCGACA GTGAGCTCCC ATCCTACCAT 
CAAAATGATG TCAGCCTAGA CCGAGGGTAC ACTTCCGACT CAGAAGTCTA CACTGACCAT 
GGCAGGCCTG GCAAGATGCA CCGATCAGCC ACAGACGCTG ACATGGCCAA CAGTGGTTGG 
TTAGTGGTGG GGAAGGATGA CATTGATAAC TCCAAACCAG CAGCAGGACT CAGCAGGCCA 
GGCCCTTCGC CCTTGGTCAA TCAGTACAGC CTTACAGTGG GCCTGGACCT GGGACCACAT 
GACACTAAGT CCTTGCTTAA GTGTGTGGAG TCTCTGTCCT TCATTGTTCG TGATGCTGCT 
CACATCACCC CTGACAACTT TGAACTCTGC GTCAAGACCC TCCGCATCTT TGTAGAAGCC 
AGTCTGAATG GTGGGTGCAA GTCCCAGGAT AAACGTGGCA AGAGTCATAA ATATGACAGC 
AAAGGGAACC GGTTCAAGAA AAAGCCCAAG GAGGGCTCGA TGCTTCGGCG GCCCCGGGCA 
TCCAGCCAGC ATGCCACTCG GGCTGGACAC AGTGATGAAG AGGAGGACGA AGGCGTGCCT 
GCCAGCTACC ATACGGTGTC TTTACAGTTG CTAGACCTGA TGCACACCCT GCACACACGG 
GCAGCCTCTA TCTACAGTTC ATGGGCAGAG GAGCAACGCC ACCTGGAGAC AGGTGGCCGG 
AAGATTGAAG CTGACTCACG TACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGT 
ATCGCTTGCT TATGCTGTGA CGCCCGGCGC CAGGTGCGGA TGCAGGCCCT GACATATCTC 
CAGCGAGCCC TTCTGGTACA TGACCTGCAA AAGCTAGATG CCCTGGAATG GGAGTCCTGT 
TTTAACAAGG TGCTGTTTCC GCTACTGACC AAGCTTTTAG AAAATATCAG CCCTGCGGAT 
GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG 
CAGCACCTGT CCCCACTGCT GTCCCTGTCC ACCTTTGCTG CCCTGTGGCT CACCATCCTG 
GACTTCATGG ACAAGTACAT GCACGCAGGT TCCAGTGACT TGCTGTCAGA AGCTGTCCCT 
GAGTCTCTGA AGAACATGCT CCTTGTGATG GACACAGCAG AGATTTTCCA CAGTGCAGAT 
GCAAGGGGAG GCAGCCCCTC TGCTCTCTGG GAGATCACCT GGGAGCGCAT CGATTGCTTC 
TTACCACACC TACGTGATGA ACTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCCACA 
GAGCCTCACA GTCAAAAACC TCTGGCTTCT ACTCACCTGA CGCCTGCTGC TGGTGACCCC 
AGGATGCCTG GCCATCCACC TCCTCCAGAG ATACCCTCAG AAGTGGGAGC TTGTGACCCG 
GAGAAGCCCG AGAGCACCAG AGCTGCCAGC AGCAGCTCCC CTGGATCGCC GGTGGCCTCT 
AGCCCCAGCA AACAGAGTCC TGCCCCAGAG GGACCTCCCC CACTGGCCCA GCCCCCACTA 
ATCCTACAGC CCTTGACCTC CCCACTGCAG GTGGGGGTAC CACCCATGAC TTTGCCCATC 
ATCCTCAACC CTGCACTCAT TGAGGCCACC TCTCCAGTGC CTCTCTTAGC CACGCCACGC 
CCTACAGACC CCATCCCCAC CTCTGAGGTC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231560.1
CDS	278..5953
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKISPCLLNTLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231498.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231498.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGA TTTCTCCATG TCTCCTCAAC 
ACACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA 
CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCAGGAGGCA TGAGTGACTC ATCCAAGTGG 
AAGAAACAGA AGCGATCCCC TCGACCTCCA CGTCATACAA CCAAAGTCAT GCCAGGTTCA 
GAGCTGCCCA CCCCAAATGG AGCCGCCTTA TCCTCTAACC TTACTGGTGG CATGCCCTTC 
ATTGAGGCAC CCACATCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCCAC AGTGGTCAGT 
CCCTGTACAG ATAGTGGCCT GGAATTATCC TCCCAGGCTA CCTCCAAGGA GGACCTCACC 
GACCTGGAGC AAGCTGGCTC CCCAAGGGAG ATCACCACCA CAGAGCCTGG GAGCACTGAG 
ATAGGAGTTT CTGATCAGCT TGACCCTCAG CAGGAAGGGG CCCATGTAGA AAAGGCCCAG 
TCAGCATCGG TGGAATCTAT CCCTGAAGTG CTAGAGGAGT GCACATCCCC TACTGACCAT 
TCTGACTCTG CCTCTGTCCA TGACATGGAT TATGTCAATC CTCGGGGCGT GCGCTTTACC 
CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTTCCTTATG GTCTTCCTTG CATCCGTGAG 
CTCTTCCGCT TCCTTATCTC CCTCACAAAT CCACATGACC GCCATAACTC TGAGGTTATG 
ATCCACATGG GACTTCATTT GCTGACAGTA GCTCTTGAGT CAGCCCCTGT GGCCCAGTGC 
CAGACCCTCT TGGGTCTCAT CAAGGATGAG ATGTGCCGAC ACTTATTCCA GCTACTCAGC 
GTAGAGCGGC TGAACCTTTA CGCTGCTTCC CTACGAGTGT GCTTTTTGCT CTTTGAGAGC 
ATGCGGGAAC ACCTCAAGTT CCAGTTGGAG ATGTACATCA AAAAGCTCAT GGAGATTATC 
ACTGTTGAAA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCGCTGGA GGCCATTGTG 
CAGCTCTGGC GCATCCCCAG CTTCGTCACG GAGCTCTACA TCAACTATGA CTGTGACTAC 
TACTGCTCCA ACCTCTTCGA AGACCTCACT AAGCTGCTGT CCAAGAATGC CTTTCCTGTG 
TCTGGCCAGC TTTATACAAC ACACCTGCTG TCTCTTGATG CCCTATTGAC AGTTATTGAC 
AGCACTGAGG CTCATTGTCA GGCCAAAGTC CTCAACACCC TCAACCAGCA AGAGAAGAAA 
GAGACAGCCA GACCTGGCTT TGAGGCAGTG GATGGCAGCC CAGACACTTA CAAGTCTGAG 
AGAGCAGCCA GTGATGGGAA AGCCACAGGA GTGCCCTCAG ATGCTCCAGG CCTTCATTTT 
TCAAGTGGAG GGTGGTTGTC AACAGAACAT GGAAAGCCAG GGTGCAGTGA TCTGGAGGAA 
GCTGGTGACT CTGGGGTTGA CAAAAAGACC ACCAGGAAGC CACCTCGATT TTCCTGTCTT 
CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AAAAGCTGCT GATCACTGGT 
ACAGAGCAGT TCAACCAAAA ACCAAAGAAG GGGATCCAGT TTCTGCAGGA GAAAGGCCTC 
CTCACCATCC CAATGGATAA CACAGAGGTA GCCCAGTGGC TCCGAGAAAA CCCTCGGCTA 
GACAAAAAAA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATGGACCT GTTGGAGAGT 
TTTGTGAGCA CCTTCAGCTT CCAGGGTCTG AGGCTTGATG AGGCTCTCCG CCTCTACCTA 
GAAGCCTTCC GTCTGCCTGG GGAAGCGCCT GTTATTCACA GGCTGCTGGA GGTGTTCACT 
GAGCACTGGA GGAGTTGTAA TGGCTCCCCA TTTGCTAATA GCGATGCCTG CTTTGCCCTG 
GCCTATGCTG TTATCATGCT TAACACTGAC CAACATAACC ACAATGTTCG CAAGCAAAAT 
GCACCCATGA CCCTGGAGGA GTTCCGCAAA AACCTGAAAG GTGTGAATGG AGGCAAGGAC 
TTTGAGCAAG ACATCCTGGA AGACATGTAC CATGCCATCA AGAATGAGGA AATTGTGATG 
CCTGAGGAAC AAACAGGTCT GGTTCGTGAG AATTATGTGT GGAGTGTGCT GCTTCATCGA 
GGTGCTACCC CTGAGGGCAT CTTCCTTCGT GTACCCCCTG GCAGCTATGA TCTTGACCTC 
TTCACTATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTGTTCGA TAAAAGCCTC 
GAGGAGACCA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGTGCCAT GATCTCCGCC 
CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTGTGCAA ATTCACAGCT 
CTAAGTAGTG AGTCTATTGA GAACCTGCCC AGTGTATTCG GAAGCAACCC TAAAGCCCAC 
ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT TCGGGAGGGC 
TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTTTT ACCGAAGGCT 
ATGGTAGAGG TAGAAGATTT TGTGGATCCC AATGGTAAGA TCTCTCTACA GCGGGAGGAG 
ACACCATCAA ACCGAGGAGA GTCAACAGTC CTTAGTTTTG TAAGCTGGCT GACACTGAGT 
GGTCCTGAGC AGTCCAGTGT CCGGGGCCCA TCGACAGAGA ACCAAGAGGC CAAGCGAGTA 
GCCTTGGACT GCATCAAGCA ATGTGACCCA GAAAAGATGA TCACAGAAAG CAAGTTCCTC 
CAGCTGGAAT CTCTGCAGGA GCTCATGAAG GCTCTGGTCT CGGTGACACC AGATGAAGAG 
ACATATGATG AAGAAGATGC TGCTTTCTGT CTGGAGATGC TGCTAAGGAT TGTGTTAGAA 
AACAGGGACC GTGTGGGCTG TGTATGGCAG ACTGTTCGAG ATCATCTATA CCACCTGTGT 
GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGAGCAG TGGTAGGGCT GCTACGCCTA 
GCCATTCGGC TACTCCGAAG AGAAGAGATT AGTGGCCAGG TCCTGCTCTC TCTGCGCATC 
TTGTTACTGA TGAAGCCCAG CGTGCTGTCC AGAGTCAGCC ACCAGGTCGC TTATGGGCTG 
CATGAACTCC TCAAGACCAA TGCTGCCAAC ATCCACTCGG GTGATGACTG GGCCACTCTC 
TTCACATTGC TGGAGTGCAT TGGCTCAGGC GTGAAGCCTC CAGATGCTCT ACAAGCCACA 
GCCAGGGCTG ATGCTCCTGA TGCTGGAGCA CAATCCGACA GTGAGCTCCC ATCCTACCAT 
CAAAATGATG TCAGCCTAGA CCGAGGGTAC ACTTCCGACT CAGAAGTCTA CACTGACCAT 
GGCAGGCCTG GCAAGATGCA CCGATCAGCC ACAGACGCTG ACATGGCCAA CAGTGGTTGG 
TTAGTGGTGG GGAAGGATGA CATTGATAAC TCCAAACCAG CAGCAGGACT CAGCAGGCCA 
GGCCCTTCGC CCTTGGTCAA TCAGTACAGC CTTACAGTGG GCCTGGACCT GGGACCACAT 
GACACTAAGT CCTTGCTTAA GTGTGTGGAG TCTCTGTCCT TCATTGTTCG TGATGCTGCT 
CACATCACCC CTGACAACTT TGAACTCTGC GTCAAGACCC TCCGCATCTT TGTAGAAGCC 
AGTCTGAATG GTGGGTGCAA GTCCCAGGAT AAACGTGGCA AGAGTCATAA ATATGACAGC 
AAAGGGAACC GGTTCAAGAA AAAGCCCAAG GAGGGCTCGA TGCTTCGGCG GCCCCGGGCA 
TCCAGCCAGC ATGCCACTCG GGCTGGACAC AGTGATGAAG AGGAGGACGA AGGCGTGCCT 
GCCAGCTACC ATACGGTGTC TTTACAGTTG CTAGACCTGA TGCACACCCT GCACACACGG 
GCAGCCTCTA TCTACAGTTC ATGGGCAGAG GAGCAACGCC ACCTGGAGAC AGGTGGCCGG 
AAGATTGAAG CTGACTCACG TACCCTCTGG GCCCACTGCT GGTGCCCTTT ATTACAAGGT 
ATCGCTTGCT TATGCTGTGA CGCCCGGCGC CAGGTGCGGA TGCAGGCCCT GACATATCTC 
CAGCGAGCCC TTCTGGTACA TGACCTGCAA AAGCTAGATG CCCTGGAATG GGAGTCCTGT 
TTTAACAAGG TGCTGTTTCC GCTACTGACC AAGCTTTTAG AAAATATCAG CCCTGCGGAT 
GTGGGCGGGA TGGAGGAGAC CCGGATGCGG GCTTCCACAC TGCTCTCAAA GGTCTTCCTG 
CAGCACCTGT CCCCACTGCT GTCCCTGTCC ACCTTTGCTG CCCTGTGGCT CACCATCCTG 
GACTTCATGG ACAAGTACAT GCACGCAGGT TCCAGTGACT TGCTGTCAGA AGCTGTCCCT 
GAGTCTCTGA AGAACATGCT CCTTGTGATG GACACAGCAG AGATTTTCCA CAGTGCAGAT 
GCAAGGGGAG GCAGCCCCTC TGCTCTCTGG GAGATCACCT GGGAGCGCAT CGATTGCTTC 
TTACCACACC TACGTGATGA ACTCTTCAAG CAGACTGTCA TCCAGGACCC CATGCCCACA 
GAGCCTCACA GTCAAAAACC TCTGGCTTCT ACTCACCTGA CGCCTGCTGC TGGTGACCCC 
AGGATGCCTG GCCATCCACC TCCTCCAGAG ATACCCTCAG AAGTGGGAGC TTGTGACCCG 
GAGAAGCCCG AGAGCACCAG AGCTGCCAGC AGCAGCTCCC CTGGATCGCC GGTGGCCTCT 
AGCCCCAGCA AACAGAGTCC TGCCCCAGAG GGACCTCCCC CACTGGCCCA GCCCCCACTA 
ATCCTACAGC CCTTGACCTC CCCACTGCAG GTGGGGGTAC CACCCATGAC TTTGCCCATC 
ATCCTCAACC CTGCACTCAT TGAGGCCACC TCTCCAGTGC CTCTCTTAGC CACGCCACGC 
CCTACAGACC CCATCCCCAC CTCTGAGGTC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231500.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231562.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa24168
Clone ID Related Accession (Same CDS sequence)	XM_006231500.3
Accession Version	XM_006231500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GCAGGAAGGG 
GCCCATGTAG AAAAGGCCCA GTCAGCATCG GTGGAATCTA TCCCTGAAGT GCTAGAGGAG 
TGCACATCCC CTACTGACCA TTCTGACTCT GCCTCTGTCC ATGACATGGA TTATGTCAAT 
CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT 
GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACATGAC 
CGCCATAACT CTGAGGTTAT GATCCACATG GGACTTCATT TGCTGACAGT AGCTCTTGAG 
TCAGCCCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TCAAGGATGA GATGTGCCGA 
CACTTATTCC AGCTACTCAG CGTAGAGCGG CTGAACCTTT ACGCTGCTTC CCTACGAGTG 
TGCTTTTTGC TCTTTGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTGGA GATGTACATC 
AAAAAGCTCA TGGAGATTAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCGCTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTCGTCAC GGAGCTCTAC 
ATCAACTATG ACTGTGACTA CTACTGCTCC AACCTCTTCG AAGACCTCAC TAAGCTGCTG 
TCCAAGAATG CCTTTCCTGT GTCTGGCCAG CTTTATACAA CACACCTGCT GTCTCTTGAT 
GCCCTATTGA CAGTTATTGA CAGCACTGAG GCTCATTGTC AGGCCAAAGT CCTCAACACC 
CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAGC 
CCAGACACTT ACAAGTCTGA GAGAGCAGCC AGTGATGGGA AAGCCACAGG AGTGCCCTCA 
GATGCTCCAG GCCTTCATTT TTCAAGTGGA GGGTGGTTGT CAACAGAACA TGGAAAGCCA 
GGGTGCAGTG ATCTGGAGGA AGCTGGTGAC TCTGGGGTTG ACAAAAAGAC CACCAGGAAG 
CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAAA ACCCTCGGCT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TCCAGGGTCT GAGGCTTGAT 
GAGGCTCTCC GCCTCTACCT AGAAGCCTTC CGTCTGCCTG GGGAAGCGCC TGTTATTCAC 
AGGCTGCTGG AGGTGTTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT 
AGCGATGCCT GCTTTGCCCT GGCCTATGCT GTTATCATGC TTAACACTGA CCAACATAAC 
CACAATGTTC GCAAGCAAAA TGCACCCATG ACCCTGGAGG AGTTCCGCAA AAACCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAGGAA CAAACAGGTC TGGTTCGTGA GAATTATGTG 
TGGAGTGTGC TGCTTCATCG AGGTGCTACC CCTGAGGGCA TCTTCCTTCG TGTACCCCCT 
GGCAGCTATG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTGTTCG ATAAAAGCCT CGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTGCC CAGTGTATTC 
GGAAGCAACC CTAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTTT TACCGAAGGC TATGGTAGAG GTAGAAGATT TTGTGGATCC CAATGGTAAG 
ATCTCTCTAC AGCGGGAGGA GACACCATCA AACCGAGGAG AGTCAACAGT CCTTAGTTTT 
GTAAGCTGGC TGACACTGAG TGGTCCTGAG CAGTCCAGTG TCCGGGGCCC ATCGACAGAG 
AACCAAGAGG CCAAGCGAGT AGCCTTGGAC TGCATCAAGC AATGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCTCTGCAGG AGCTCATGAA GGCTCTGGTC 
TCGGTGACAC CAGATGAAGA GACATATGAT GAAGAAGATG CTGCTTTCTG TCTGGAGATG 
CTGCTAAGGA TTGTGTTAGA AAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA 
GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA 
GTGGTAGGGC TGCTACGCCT AGCCATTCGG CTACTCCGAA GAGAAGAGAT TAGTGGCCAG 
GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC 
CACCAGGTCG CTTATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG 
GGTGATGACT GGGCCACTCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT 
CCAGATGCTC TACAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCTGGAGC ACAATCCGAC 
AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTAG ACCGAGGGTA CACTTCCGAC 
TCAGAAGTCT ACACTGACCA TGGCAGGCCT GGCAAGATGC ACCGATCAGC CACAGACGCT 
GACATGGCCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA 
GCAGCAGGAC TCAGCAGGCC AGGCCCTTCG CCCTTGGTCA ATCAGTACAG CCTTACAGTG 
GGCCTGGACC TGGGACCACA TGACACTAAG TCCTTGCTTA AGTGTGTGGA GTCTCTGTCC 
TTCATTGTTC GTGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACC 
CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGTGGC 
AAGAGTCATA AATATGACAG CAAAGGGAAC CGGTTCAAGA AAAAGCCCAA GGAGGGCTCG 
ATGCTTCGGC GGCCCCGGGC ATCCAGCCAG CATGCCACTC GGGCTGGACA CAGTGATGAA 
GAGGAGGACG AAGGCGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGCTAGACC TGATGCACAC CCTGCACACA CGGGCAGCCT CTATCTACAG TTCATGGGCA 
GAGGAGCAAC GCCACCTGGA GACAGGTGGC CGGAAGATTG AAGCTGACTC ACGTACCCTC 
TGGGCCCACT GCTGGTGCCC TTTATTACAA GGTATCGCTT GCTTATGCTG TGACGCCCGG 
CGCCAGGTGC GGATGCAGGC CCTGACATAT CTCCAGCGAG CCCTTCTGGT ACATGACCTG 
CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCGCTACTG 
ACCAAGCTTT TAGAAAATAT CAGCCCTGCG GATGTGGGCG GGATGGAGGA GACCCGGATG 
CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCACC TGTCCCCACT GCTGTCCCTG 
TCCACCTTTG CTGCCCTGTG GCTCACCATC CTGGACTTCA TGGACAAGTA CATGCACGCA 
GGTTCCAGTG ACTTGCTGTC AGAAGCTGTC CCTGAGTCTC TGAAGAACAT GCTCCTTGTG 
ATGGACACAG CAGAGATTTT CCACAGTGCA GATGCAAGGG GAGGCAGCCC CTCTGCTCTC 
TGGGAGATCA CCTGGGAGCG CATCGATTGC TTCTTACCAC ACCTACGTGA TGAACTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCC ACAGAGCCTC ACAGTCAAAA ACCTCTGGCT 
TCTACTCACC TGACGCCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCCTCCA 
GAGATACCCT CAGAAGTGGG AGCTTGTGAC CCGGAGAAGC CCGAGAGCAC CAGAGCTGCC 
AGCAGCAGCT CCCCTGGATC GCCGGTGGCC TCTAGCCCCA GCAAACAGAG TCCTGCCCCA 
GAGGGACCTC CCCCACTGGC CCAGCCCCCA CTAATCCTAC AGCCCTTGAC CTCCCCACTG 
CAGGTGGGGG TACCACCCAT GACTTTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC 
ACCTCTCCAG TGCCTCTCTT AGCCACGCCA CGCCCTACAG ACCCCATCCC CACCTCTGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231562.1
CDS	260..5848
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231500.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231500.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GCAGGAAGGG 
GCCCATGTAG AAAAGGCCCA GTCAGCATCG GTGGAATCTA TCCCTGAAGT GCTAGAGGAG 
TGCACATCCC CTACTGACCA TTCTGACTCT GCCTCTGTCC ATGACATGGA TTATGTCAAT 
CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT 
GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACATGAC 
CGCCATAACT CTGAGGTTAT GATCCACATG GGACTTCATT TGCTGACAGT AGCTCTTGAG 
TCAGCCCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TCAAGGATGA GATGTGCCGA 
CACTTATTCC AGCTACTCAG CGTAGAGCGG CTGAACCTTT ACGCTGCTTC CCTACGAGTG 
TGCTTTTTGC TCTTTGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTGGA GATGTACATC 
AAAAAGCTCA TGGAGATTAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCGCTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTCGTCAC GGAGCTCTAC 
ATCAACTATG ACTGTGACTA CTACTGCTCC AACCTCTTCG AAGACCTCAC TAAGCTGCTG 
TCCAAGAATG CCTTTCCTGT GTCTGGCCAG CTTTATACAA CACACCTGCT GTCTCTTGAT 
GCCCTATTGA CAGTTATTGA CAGCACTGAG GCTCATTGTC AGGCCAAAGT CCTCAACACC 
CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAGC 
CCAGACACTT ACAAGTCTGA GAGAGCAGCC AGTGATGGGA AAGCCACAGG AGTGCCCTCA 
GATGCTCCAG GCCTTCATTT TTCAAGTGGA GGGTGGTTGT CAACAGAACA TGGAAAGCCA 
GGGTGCAGTG ATCTGGAGGA AGCTGGTGAC TCTGGGGTTG ACAAAAAGAC CACCAGGAAG 
CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAAA ACCCTCGGCT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TCCAGGGTCT GAGGCTTGAT 
GAGGCTCTCC GCCTCTACCT AGAAGCCTTC CGTCTGCCTG GGGAAGCGCC TGTTATTCAC 
AGGCTGCTGG AGGTGTTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT 
AGCGATGCCT GCTTTGCCCT GGCCTATGCT GTTATCATGC TTAACACTGA CCAACATAAC 
CACAATGTTC GCAAGCAAAA TGCACCCATG ACCCTGGAGG AGTTCCGCAA AAACCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAGGAA CAAACAGGTC TGGTTCGTGA GAATTATGTG 
TGGAGTGTGC TGCTTCATCG AGGTGCTACC CCTGAGGGCA TCTTCCTTCG TGTACCCCCT 
GGCAGCTATG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTGTTCG ATAAAAGCCT CGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTGCC CAGTGTATTC 
GGAAGCAACC CTAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTTT TACCGAAGGC TATGGTAGAG GTAGAAGATT TTGTGGATCC CAATGGTAAG 
ATCTCTCTAC AGCGGGAGGA GACACCATCA AACCGAGGAG AGTCAACAGT CCTTAGTTTT 
GTAAGCTGGC TGACACTGAG TGGTCCTGAG CAGTCCAGTG TCCGGGGCCC ATCGACAGAG 
AACCAAGAGG CCAAGCGAGT AGCCTTGGAC TGCATCAAGC AATGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCTCTGCAGG AGCTCATGAA GGCTCTGGTC 
TCGGTGACAC CAGATGAAGA GACATATGAT GAAGAAGATG CTGCTTTCTG TCTGGAGATG 
CTGCTAAGGA TTGTGTTAGA AAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA 
GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA 
GTGGTAGGGC TGCTACGCCT AGCCATTCGG CTACTCCGAA GAGAAGAGAT TAGTGGCCAG 
GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC 
CACCAGGTCG CTTATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG 
GGTGATGACT GGGCCACTCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT 
CCAGATGCTC TACAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCTGGAGC ACAATCCGAC 
AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTAG ACCGAGGGTA CACTTCCGAC 
TCAGAAGTCT ACACTGACCA TGGCAGGCCT GGCAAGATGC ACCGATCAGC CACAGACGCT 
GACATGGCCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA 
GCAGCAGGAC TCAGCAGGCC AGGCCCTTCG CCCTTGGTCA ATCAGTACAG CCTTACAGTG 
GGCCTGGACC TGGGACCACA TGACACTAAG TCCTTGCTTA AGTGTGTGGA GTCTCTGTCC 
TTCATTGTTC GTGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACC 
CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGTGGC 
AAGAGTCATA AATATGACAG CAAAGGGAAC CGGTTCAAGA AAAAGCCCAA GGAGGGCTCG 
ATGCTTCGGC GGCCCCGGGC ATCCAGCCAG CATGCCACTC GGGCTGGACA CAGTGATGAA 
GAGGAGGACG AAGGCGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGCTAGACC TGATGCACAC CCTGCACACA CGGGCAGCCT CTATCTACAG TTCATGGGCA 
GAGGAGCAAC GCCACCTGGA GACAGGTGGC CGGAAGATTG AAGCTGACTC ACGTACCCTC 
TGGGCCCACT GCTGGTGCCC TTTATTACAA GGTATCGCTT GCTTATGCTG TGACGCCCGG 
CGCCAGGTGC GGATGCAGGC CCTGACATAT CTCCAGCGAG CCCTTCTGGT ACATGACCTG 
CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCGCTACTG 
ACCAAGCTTT TAGAAAATAT CAGCCCTGCG GATGTGGGCG GGATGGAGGA GACCCGGATG 
CGGGCTTCCA CACTGCTCTC AAAGGTCTTC CTGCAGCACC TGTCCCCACT GCTGTCCCTG 
TCCACCTTTG CTGCCCTGTG GCTCACCATC CTGGACTTCA TGGACAAGTA CATGCACGCA 
GGTTCCAGTG ACTTGCTGTC AGAAGCTGTC CCTGAGTCTC TGAAGAACAT GCTCCTTGTG 
ATGGACACAG CAGAGATTTT CCACAGTGCA GATGCAAGGG GAGGCAGCCC CTCTGCTCTC 
TGGGAGATCA CCTGGGAGCG CATCGATTGC TTCTTACCAC ACCTACGTGA TGAACTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCC ACAGAGCCTC ACAGTCAAAA ACCTCTGGCT 
TCTACTCACC TGACGCCTGC TGCTGGTGAC CCCAGGATGC CTGGCCATCC ACCTCCTCCA 
GAGATACCCT CAGAAGTGGG AGCTTGTGAC CCGGAGAAGC CCGAGAGCAC CAGAGCTGCC 
AGCAGCAGCT CCCCTGGATC GCCGGTGGCC TCTAGCCCCA GCAAACAGAG TCCTGCCCCA 
GAGGGACCTC CCCCACTGGC CCAGCCCCCA CTAATCCTAC AGCCCTTGAC CTCCCCACTG 
CAGGTGGGGG TACCACCCAT GACTTTGCCC ATCATCCTCA ACCCTGCACT CATTGAGGCC 
ACCTCTCCAG TGCCTCTCTT AGCCACGCCA CGCCCTACAG ACCCCATCCC CACCTCTGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231501.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231563.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X5, mRNA.

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CloneID	ORa24169
Clone ID Related Accession (Same CDS sequence)	XM_006231501.3
Accession Version	XM_006231501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GCAGGAAGGG 
GCCCATGTAG AAAAGGCCCA GTCAGCATCG GTGGAATCTA TCCCTGAAGT GCTAGAGGAG 
TGCACATCCC CTACTGACCA TTCTGACTCT GCCTCTGTCC ATGACATGGA TTATGTCAAT 
CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT 
GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACATGAC 
CGCCATAACT CTGAGGTTAT GATCCACATG GGACTTCATT TGCTGACAGT AGCTCTTGAG 
TCAGCCCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TCAAGGATGA GATGTGCCGA 
CACTTATTCC AGCTACTCAG CGTAGAGCGG CTGAACCTTT ACGCTGCTTC CCTACGAGTG 
TGCTTTTTGC TCTTTGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTGGA GATGTACATC 
AAAAAGCTCA TGGAGATTAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCGCTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTCGTCAC GGAGCTCTAC 
ATCAACTATG ACTGTGACTA CTACTGCTCC AACCTCTTCG AAGACCTCAC TAAGCTGCTG 
TCCAAGAATG CCTTTCCTGT GTCTGGCCAG CTTTATACAA CACACCTGCT GTCTCTTGAT 
GCCCTATTGA CAGTTATTGA CAGCACTGAG GCTCATTGTC AGGCCAAAGT CCTCAACACC 
CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAGC 
CCAGACACTT ACAAGTCTGA GAGAGCAGCC AGTGATGGGA AAGCCACAGG AGTGCCCTCA 
GATGCTCCAG GCCTTCATTT TTCAAGTGGA GGGTGGTTGT CAACAGAACA TGGAAAGCCA 
GGGTGCAGTG ATCTGGAGGA AGCTGGTGAC TCTGGGGTTG ACAAAAAGAC CACCAGGAAG 
CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAAA ACCCTCGGCT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TCCAGGGTCT GAGGCTTGAT 
GAGGCTCTCC GCCTCTACCT AGAAGCCTTC CGTCTGCCTG GGGAAGCGCC TGTTATTCAC 
AGGCTGCTGG AGGTGTTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT 
AGCGATGCCT GCTTTGCCCT GGCCTATGCT GTTATCATGC TTAACACTGA CCAACATAAC 
CACAATGTTC GCAAGCAAAA TGCACCCATG ACCCTGGAGG AGTTCCGCAA AAACCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAGGAA CAAACAGGTC TGGTTCGTGA GAATTATGTG 
TGGAGTGTGC TGCTTCATCG AGGTGCTACC CCTGAGGGCA TCTTCCTTCG TGTACCCCCT 
GGCAGCTATG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTGTTCG ATAAAAGCCT CGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTGCC CAGTGTATTC 
GGAAGCAACC CTAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTTT TACCGAAGGC TATGGTAGAG GTAGAAGATT TTGTGGATCC CAATGGTAAG 
ATCTCTCTAC AGCGGGAGGA GACACCATCA AACCGAGGAG AGTCAACAGT CCTTAGTTTT 
GTAAGCTGGC TGACACTGAG TGGTCCTGAG CAGTCCAGTG TCCGGGGCCC ATCGACAGAG 
AACCAAGAGG CCAAGCGAGT AGCCTTGGAC TGCATCAAGC AATGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCTCTGCAGG AGCTCATGAA GGCTCTGGTC 
TCGGTGACAC CAGATGAAGA GACATATGAT GAAGAAGATG CTGCTTTCTG TCTGGAGATG 
CTGCTAAGGA TTGTGTTAGA AAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA 
GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA 
GTGGTAGGGC TGCTACGCCT AGCCATTCGG CTACTCCGAA GAGAAGAGAT TAGTGGCCAG 
GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC 
CACCAGGTCG CTTATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG 
GGTGATGACT GGGCCACTCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT 
CCAGATGCTC TACAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCTGGAGC ACAATCCGAC 
AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTAG ACCGAGGGTA CACTTCCGAC 
TCAGAAGTCT ACACTGACCA TGGCAGGCCT GGCAAGATGC ACCGATCAGC CACAGACGCT 
GACATGGCCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA 
GCAGCAGGAC TCAGCAGGCC AGGCCCTTCG CCCTTGGTCA ATCAGTACAG CCTTACAGTG 
GGCCTGGACC TGGGACCACA TGACACTAAG TCCTTGCTTA AGTGTGTGGA GTCTCTGTCC 
TTCATTGTTC GTGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACC 
CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGTGGC 
AAGAGTCATA AATATGACAG CAAAGGGAAC CGGTTCAAGA AAAAGCCCAA GGAGGGCTCG 
ATGCTTCGGC GGCCCCGGGC ATCCAGCCAG CATGCCACTC GGGCTGGACA CAGTGATGAA 
GAGGAGGACG AAGGCGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG 
ATGCACACCC TGCACACACG GGCAGCCTCT ATCTACAGTT CATGGGCAGA GGAGCAACGC 
CACCTGGAGA CAGGTGGCCG GAAGATTGAA GCTGACTCAC GTACCCTCTG GGCCCACTGC 
TGGTGCCCTT TATTACAAGG TATCGCTTGC TTATGCTGTG ACGCCCGGCG CCAGGTGCGG 
ATGCAGGCCC TGACATATCT CCAGCGAGCC CTTCTGGTAC ATGACCTGCA AAAGCTAGAT 
GCCCTGGAAT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CGCTACTGAC CAAGCTTTTA 
GAAAATATCA GCCCTGCGGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA 
CTGCTCTCAA AGGTCTTCCT GCAGCACCTG TCCCCACTGC TGTCCCTGTC CACCTTTGCT 
GCCCTGTGGC TCACCATCCT GGACTTCATG GACAAGTACA TGCACGCAGG TTCCAGTGAC 
TTGCTGTCAG AAGCTGTCCC TGAGTCTCTG AAGAACATGC TCCTTGTGAT GGACACAGCA 
GAGATTTTCC ACAGTGCAGA TGCAAGGGGA GGCAGCCCCT CTGCTCTCTG GGAGATCACC 
TGGGAGCGCA TCGATTGCTT CTTACCACAC CTACGTGATG AACTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCCAC AGAGCCTCAC AGTCAAAAAC CTCTGGCTTC TACTCACCTG 
ACGCCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCCTCCAGA GATACCCTCA 
GAAGTGGGAG CTTGTGACCC GGAGAAGCCC GAGAGCACCA GAGCTGCCAG CAGCAGCTCC 
CCTGGATCGC CGGTGGCCTC TAGCCCCAGC AAACAGAGTC CTGCCCCAGA GGGACCTCCC 
CCACTGGCCC AGCCCCCACT AATCCTACAG CCCTTGACCT CCCCACTGCA GGTGGGGGTA 
CCACCCATGA CTTTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCTCCAGTG 
CCTCTCTTAG CCACGCCACG CCCTACAGAC CCCATCCCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231563.1
CDS	260..5836
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231501.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231501.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GCAGGAAGGG 
GCCCATGTAG AAAAGGCCCA GTCAGCATCG GTGGAATCTA TCCCTGAAGT GCTAGAGGAG 
TGCACATCCC CTACTGACCA TTCTGACTCT GCCTCTGTCC ATGACATGGA TTATGTCAAT 
CCTCGGGGCG TGCGCTTTAC CCAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTTCCTTAT 
GGTCTTCCTT GCATCCGTGA GCTCTTCCGC TTCCTTATCT CCCTCACAAA TCCACATGAC 
CGCCATAACT CTGAGGTTAT GATCCACATG GGACTTCATT TGCTGACAGT AGCTCTTGAG 
TCAGCCCCTG TGGCCCAGTG CCAGACCCTC TTGGGTCTCA TCAAGGATGA GATGTGCCGA 
CACTTATTCC AGCTACTCAG CGTAGAGCGG CTGAACCTTT ACGCTGCTTC CCTACGAGTG 
TGCTTTTTGC TCTTTGAGAG CATGCGGGAA CACCTCAAGT TCCAGTTGGA GATGTACATC 
AAAAAGCTCA TGGAGATTAT CACTGTTGAA AACCCCAAGA TGCCTTATGA GATGAAGGAG 
ATGGCGCTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTCGTCAC GGAGCTCTAC 
ATCAACTATG ACTGTGACTA CTACTGCTCC AACCTCTTCG AAGACCTCAC TAAGCTGCTG 
TCCAAGAATG CCTTTCCTGT GTCTGGCCAG CTTTATACAA CACACCTGCT GTCTCTTGAT 
GCCCTATTGA CAGTTATTGA CAGCACTGAG GCTCATTGTC AGGCCAAAGT CCTCAACACC 
CTCAACCAGC AAGAGAAGAA AGAGACAGCC AGACCTGGCT TTGAGGCAGT GGATGGCAGC 
CCAGACACTT ACAAGTCTGA GAGAGCAGCC AGTGATGGGA AAGCCACAGG AGTGCCCTCA 
GATGCTCCAG GCCTTCATTT TTCAAGTGGA GGGTGGTTGT CAACAGAACA TGGAAAGCCA 
GGGTGCAGTG ATCTGGAGGA AGCTGGTGAC TCTGGGGTTG ACAAAAAGAC CACCAGGAAG 
CCACCTCGAT TTTCCTGTCT TCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA 
AAAAAGCTGC TGATCACTGG TACAGAGCAG TTCAACCAAA AACCAAAGAA GGGGATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGATA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAAA ACCCTCGGCT AGACAAAAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATGGACC TGTTGGAGAG TTTTGTGAGC ACCTTCAGCT TCCAGGGTCT GAGGCTTGAT 
GAGGCTCTCC GCCTCTACCT AGAAGCCTTC CGTCTGCCTG GGGAAGCGCC TGTTATTCAC 
AGGCTGCTGG AGGTGTTCAC TGAGCACTGG AGGAGTTGTA ATGGCTCCCC ATTTGCTAAT 
AGCGATGCCT GCTTTGCCCT GGCCTATGCT GTTATCATGC TTAACACTGA CCAACATAAC 
CACAATGTTC GCAAGCAAAA TGCACCCATG ACCCTGGAGG AGTTCCGCAA AAACCTGAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AAGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAGGAA CAAACAGGTC TGGTTCGTGA GAATTATGTG 
TGGAGTGTGC TGCTTCATCG AGGTGCTACC CCTGAGGGCA TCTTCCTTCG TGTACCCCCT 
GGCAGCTATG ATCTTGACCT CTTCACTATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT 
TATGTGTTCG ATAAAAGCCT CGAGGAGACC ATCATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGTGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC 
TCTCTGTGCA AATTCACAGC TCTAAGTAGT GAGTCTATTG AGAACCTGCC CAGTGTATTC 
GGAAGCAACC CTAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGACATCC TTCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA 
GCCCAACTTT TACCGAAGGC TATGGTAGAG GTAGAAGATT TTGTGGATCC CAATGGTAAG 
ATCTCTCTAC AGCGGGAGGA GACACCATCA AACCGAGGAG AGTCAACAGT CCTTAGTTTT 
GTAAGCTGGC TGACACTGAG TGGTCCTGAG CAGTCCAGTG TCCGGGGCCC ATCGACAGAG 
AACCAAGAGG CCAAGCGAGT AGCCTTGGAC TGCATCAAGC AATGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT CCAGCTGGAA TCTCTGCAGG AGCTCATGAA GGCTCTGGTC 
TCGGTGACAC CAGATGAAGA GACATATGAT GAAGAAGATG CTGCTTTCTG TCTGGAGATG 
CTGCTAAGGA TTGTGTTAGA AAACAGGGAC CGTGTGGGCT GTGTATGGCA GACTGTTCGA 
GATCATCTAT ACCACCTGTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGAGCA 
GTGGTAGGGC TGCTACGCCT AGCCATTCGG CTACTCCGAA GAGAAGAGAT TAGTGGCCAG 
GTCCTGCTCT CTCTGCGCAT CTTGTTACTG ATGAAGCCCA GCGTGCTGTC CAGAGTCAGC 
CACCAGGTCG CTTATGGGCT GCATGAACTC CTCAAGACCA ATGCTGCCAA CATCCACTCG 
GGTGATGACT GGGCCACTCT CTTCACATTG CTGGAGTGCA TTGGCTCAGG CGTGAAGCCT 
CCAGATGCTC TACAAGCCAC AGCCAGGGCT GATGCTCCTG ATGCTGGAGC ACAATCCGAC 
AGTGAGCTCC CATCCTACCA TCAAAATGAT GTCAGCCTAG ACCGAGGGTA CACTTCCGAC 
TCAGAAGTCT ACACTGACCA TGGCAGGCCT GGCAAGATGC ACCGATCAGC CACAGACGCT 
GACATGGCCA ACAGTGGTTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAACCA 
GCAGCAGGAC TCAGCAGGCC AGGCCCTTCG CCCTTGGTCA ATCAGTACAG CCTTACAGTG 
GGCCTGGACC TGGGACCACA TGACACTAAG TCCTTGCTTA AGTGTGTGGA GTCTCTGTCC 
TTCATTGTTC GTGATGCTGC TCACATCACC CCTGACAACT TTGAACTCTG CGTCAAGACC 
CTCCGCATCT TTGTAGAAGC CAGTCTGAAT GGTGGGTGCA AGTCCCAGGA TAAACGTGGC 
AAGAGTCATA AATATGACAG CAAAGGGAAC CGGTTCAAGA AAAAGCCCAA GGAGGGCTCG 
ATGCTTCGGC GGCCCCGGGC ATCCAGCCAG CATGCCACTC GGGCTGGACA CAGTGATGAA 
GAGGAGGACG AAGGCGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GCTAGACCTG 
ATGCACACCC TGCACACACG GGCAGCCTCT ATCTACAGTT CATGGGCAGA GGAGCAACGC 
CACCTGGAGA CAGGTGGCCG GAAGATTGAA GCTGACTCAC GTACCCTCTG GGCCCACTGC 
TGGTGCCCTT TATTACAAGG TATCGCTTGC TTATGCTGTG ACGCCCGGCG CCAGGTGCGG 
ATGCAGGCCC TGACATATCT CCAGCGAGCC CTTCTGGTAC ATGACCTGCA AAAGCTAGAT 
GCCCTGGAAT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CGCTACTGAC CAAGCTTTTA 
GAAAATATCA GCCCTGCGGA TGTGGGCGGG ATGGAGGAGA CCCGGATGCG GGCTTCCACA 
CTGCTCTCAA AGGTCTTCCT GCAGCACCTG TCCCCACTGC TGTCCCTGTC CACCTTTGCT 
GCCCTGTGGC TCACCATCCT GGACTTCATG GACAAGTACA TGCACGCAGG TTCCAGTGAC 
TTGCTGTCAG AAGCTGTCCC TGAGTCTCTG AAGAACATGC TCCTTGTGAT GGACACAGCA 
GAGATTTTCC ACAGTGCAGA TGCAAGGGGA GGCAGCCCCT CTGCTCTCTG GGAGATCACC 
TGGGAGCGCA TCGATTGCTT CTTACCACAC CTACGTGATG AACTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCCAC AGAGCCTCAC AGTCAAAAAC CTCTGGCTTC TACTCACCTG 
ACGCCTGCTG CTGGTGACCC CAGGATGCCT GGCCATCCAC CTCCTCCAGA GATACCCTCA 
GAAGTGGGAG CTTGTGACCC GGAGAAGCCC GAGAGCACCA GAGCTGCCAG CAGCAGCTCC 
CCTGGATCGC CGGTGGCCTC TAGCCCCAGC AAACAGAGTC CTGCCCCAGA GGGACCTCCC 
CCACTGGCCC AGCCCCCACT AATCCTACAG CCCTTGACCT CCCCACTGCA GGTGGGGGTA 
CCACCCATGA CTTTGCCCAT CATCCTCAAC CCTGCACTCA TTGAGGCCAC CTCTCCAGTG 
CCTCTCTTAG CCACGCCACG CCCTACAGAC CCCATCCCCA CCTCTGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gbf1 ( XM_006231502.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231564.1 Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X6, mRNA.

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CloneID	ORa24170
Clone ID Related Accession (Same CDS sequence)	XM_006231502.3
Accession Version	XM_006231502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GGAAGGGGCC 
CATGTAGAAA AGGCCCAGTC AGCATCGGTG GAATCTATCC CTGAAGTGCT AGAGGAGTGC 
ACATCCCCTA CTGACCATTC TGACTCTGCC TCTGTCCATG ACATGGATTA TGTCAATCCT 
CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT 
CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACATGACCGC 
CATAACTCTG AGGTTATGAT CCACATGGGA CTTCATTTGC TGACAGTAGC TCTTGAGTCA 
GCCCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATCA AGGATGAGAT GTGCCGACAC 
TTATTCCAGC TACTCAGCGT AGAGCGGCTG AACCTTTACG CTGCTTCCCT ACGAGTGTGC 
TTTTTGCTCT TTGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTGGAGAT GTACATCAAA 
AAGCTCATGG AGATTATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCGCTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TCGTCACGGA GCTCTACATC 
AACTATGACT GTGACTACTA CTGCTCCAAC CTCTTCGAAG ACCTCACTAA GCTGCTGTCC 
AAGAATGCCT TTCCTGTGTC TGGCCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC 
CTATTGACAG TTATTGACAG CACTGAGGCT CATTGTCAGG CCAAAGTCCT CAACACCCTC 
AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAGCCCA 
GACACTTACA AGTCTGAGAG AGCAGCCAGT GATGGGAAAG CCACAGGAGT GCCCTCAGAT 
GCTCCAGGCC TTCATTTTTC AAGTGGAGGG TGGTTGTCAA CAGAACATGG AAAGCCAGGG 
TGCAGTGATC TGGAGGAAGC TGGTGACTCT GGGGTTGACA AAAAGACCAC CAGGAAGCCA 
CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA 
AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAAAACC CTCGGCTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTCC AGGGTCTGAG GCTTGATGAG 
GCTCTCCGCC TCTACCTAGA AGCCTTCCGT CTGCCTGGGG AAGCGCCTGT TATTCACAGG 
CTGCTGGAGG TGTTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGC 
GATGCCTGCT TTGCCCTGGC CTATGCTGTT ATCATGCTTA ACACTGACCA ACATAACCAC 
AATGTTCGCA AGCAAAATGC ACCCATGACC CTGGAGGAGT TCCGCAAAAA CCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAGGAACAA ACAGGTCTGG TTCGTGAGAA TTATGTGTGG 
AGTGTGCTGC TTCATCGAGG TGCTACCCCT GAGGGCATCT TCCTTCGTGT ACCCCCTGGC 
AGCTATGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTGTTCGATA AAAGCCTCGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTCGGA 
AGCAACCCTA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTTTTAC CGAAGGCTAT GGTAGAGGTA GAAGATTTTG TGGATCCCAA TGGTAAGATC 
TCTCTACAGC GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTCCT TAGTTTTGTA 
AGCTGGCTGA CACTGAGTGG TCCTGAGCAG TCCAGTGTCC GGGGCCCATC GACAGAGAAC 
CAAGAGGCCA AGCGAGTAGC CTTGGACTGC ATCAAGCAAT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAATCT CTGCAGGAGC TCATGAAGGC TCTGGTCTCG 
GTGACACCAG ATGAAGAGAC ATATGATGAA GAAGATGCTG CTTTCTGTCT GGAGATGCTG 
CTAAGGATTG TGTTAGAAAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT 
CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG 
GTAGGGCTGC TACGCCTAGC CATTCGGCTA CTCCGAAGAG AAGAGATTAG TGGCCAGGTC 
CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC 
CAGGTCGCTT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT 
GATGACTGGG CCACTCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA 
GATGCTCTAC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CTGGAGCACA ATCCGACAGT 
GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTAGACC GAGGGTACAC TTCCGACTCA 
GAAGTCTACA CTGACCATGG CAGGCCTGGC AAGATGCACC GATCAGCCAC AGACGCTGAC 
ATGGCCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGCA 
GCAGGACTCA GCAGGCCAGG CCCTTCGCCC TTGGTCAATC AGTACAGCCT TACAGTGGGC 
CTGGACCTGG GACCACATGA CACTAAGTCC TTGCTTAAGT GTGTGGAGTC TCTGTCCTTC 
ATTGTTCGTG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACCCTC 
CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGTGGCAAG 
AGTCATAAAT ATGACAGCAA AGGGAACCGG TTCAAGAAAA AGCCCAAGGA GGGCTCGATG 
CTTCGGCGGC CCCGGGCATC CAGCCAGCAT GCCACTCGGG CTGGACACAG TGATGAAGAG 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACACGGGC AGCCTCTATC TACAGTTCAT GGGCAGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCGGAA GATTGAAGCT GACTCACGTA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAT TACAAGGTAT CGCTTGCTTA TGCTGTGACG CCCGGCGCCA GGTGCGGATG 
CAGGCCCTGA CATATCTCCA GCGAGCCCTT CTGGTACATG ACCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCGC TACTGACCAA GCTTTTAGAA 
AATATCAGCC CTGCGGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG 
CTCTCAAAGG TCTTCCTGCA GCACCTGTCC CCACTGCTGT CCCTGTCCAC CTTTGCTGCC 
CTGTGGCTCA CCATCCTGGA CTTCATGGAC AAGTACATGC ACGCAGGTTC CAGTGACTTG 
CTGTCAGAAG CTGTCCCTGA GTCTCTGAAG AACATGCTCC TTGTGATGGA CACAGCAGAG 
ATTTTCCACA GTGCAGATGC AAGGGGAGGC AGCCCCTCTG CTCTCTGGGA GATCACCTGG 
GAGCGCATCG ATTGCTTCTT ACCACACCTA CGTGATGAAC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCCACAGA GCCTCACAGT CAAAAACCTC TGGCTTCTAC TCACCTGACG 
CCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC CTCCAGAGAT ACCCTCAGAA 
GTGGGAGCTT GTGACCCGGA GAAGCCCGAG AGCACCAGAG CTGCCAGCAG CAGCTCCCCT 
GGATCGCCGG TGGCCTCTAG CCCCAGCAAA CAGAGTCCTG CCCCAGAGGG ACCTCCCCCA 
CTGGCCCAGC CCCCACTAAT CCTACAGCCC TTGACCTCCC CACTGCAGGT GGGGGTACCA 
CCCATGACTT TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC TCCAGTGCCT 
CTCTTAGCCA CGCCACGCCC TACAGACCCC ATCCCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231564.1
CDS	278..5851
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Target ORF information:

RefSeq Version	XM_006231502.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (Gbf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231502.3

ORF Insert Sequence:

ATGGTGGATA AGAATATTTA CATCATTCAA GGAGAAATTA ACATTGTTGT TGGTGCCATC 
AAACGAAATG CCCGATGGAG CACCCATATA CCACTGGATG AAGAGCGGGA TCCCCTTTTG 
CACAGTTTCA GCCATCTAAA GGAGGTCTTA AACAGTGGGA CAGAACTCTC AGAGATTGAG 
CCAAATGTAT TCCTTCGTCC ATTTCTGGAA GTCATTCGCT CTGAAGACAC CACTGGTCCT 
ATCACCGGCC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTGTGCACT CATAGATCCA 
ACTCATGAGG GCACGGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTC 
CGGACTTTGT TGCTAACCCC AGTGGGTACC CACTTAACAA ATGAATCTGT ATGTGAGATT 
ATGCAGTCTT GCTTCCGGAT TTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC GCAGTTTAAA 
GAAGAACCCA AGAGTTACGT GGGAACCAAC TTGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGACT CATCCAAGTG GAAGAAACAG AAGCGATCCC CTCGACCTCC ACGTCATACA 
ACCAAAGTCA TGCCAGGTTC AGAGCTGCCC ACCCCAAATG GAGCCGCCTT ATCCTCTAAC 
CTTACTGGTG GCATGCCCTT CATTGAGGCA CCCACATCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCCA CAGTGGTCAG TCCCTGTACA GATAGTGGCC TGGAATTATC CTCCCAGGCT 
ACCTCCAAGG AGGACCTCAC CGACCTGGAG CAAGCTGGCT CCCCAAGGGA GATCACCACC 
ACAGAGCCTG GGAGCACTGA GATAGGAGTT TCTGATCAGC TTGACCCTCA GGAAGGGGCC 
CATGTAGAAA AGGCCCAGTC AGCATCGGTG GAATCTATCC CTGAAGTGCT AGAGGAGTGC 
ACATCCCCTA CTGACCATTC TGACTCTGCC TCTGTCCATG ACATGGATTA TGTCAATCCT 
CGGGGCGTGC GCTTTACCCA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT TCCTTATGGT 
CTTCCTTGCA TCCGTGAGCT CTTCCGCTTC CTTATCTCCC TCACAAATCC ACATGACCGC 
CATAACTCTG AGGTTATGAT CCACATGGGA CTTCATTTGC TGACAGTAGC TCTTGAGTCA 
GCCCCTGTGG CCCAGTGCCA GACCCTCTTG GGTCTCATCA AGGATGAGAT GTGCCGACAC 
TTATTCCAGC TACTCAGCGT AGAGCGGCTG AACCTTTACG CTGCTTCCCT ACGAGTGTGC 
TTTTTGCTCT TTGAGAGCAT GCGGGAACAC CTCAAGTTCC AGTTGGAGAT GTACATCAAA 
AAGCTCATGG AGATTATCAC TGTTGAAAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG 
GCGCTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TCGTCACGGA GCTCTACATC 
AACTATGACT GTGACTACTA CTGCTCCAAC CTCTTCGAAG ACCTCACTAA GCTGCTGTCC 
AAGAATGCCT TTCCTGTGTC TGGCCAGCTT TATACAACAC ACCTGCTGTC TCTTGATGCC 
CTATTGACAG TTATTGACAG CACTGAGGCT CATTGTCAGG CCAAAGTCCT CAACACCCTC 
AACCAGCAAG AGAAGAAAGA GACAGCCAGA CCTGGCTTTG AGGCAGTGGA TGGCAGCCCA 
GACACTTACA AGTCTGAGAG AGCAGCCAGT GATGGGAAAG CCACAGGAGT GCCCTCAGAT 
GCTCCAGGCC TTCATTTTTC AAGTGGAGGG TGGTTGTCAA CAGAACATGG AAAGCCAGGG 
TGCAGTGATC TGGAGGAAGC TGGTGACTCT GGGGTTGACA AAAAGACCAC CAGGAAGCCA 
CCTCGATTTT CCTGTCTTCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAA 
AAGCTGCTGA TCACTGGTAC AGAGCAGTTC AACCAAAAAC CAAAGAAGGG GATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGATAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAAAACC CTCGGCTAGA CAAAAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATGGACCTGT TGGAGAGTTT TGTGAGCACC TTCAGCTTCC AGGGTCTGAG GCTTGATGAG 
GCTCTCCGCC TCTACCTAGA AGCCTTCCGT CTGCCTGGGG AAGCGCCTGT TATTCACAGG 
CTGCTGGAGG TGTTCACTGA GCACTGGAGG AGTTGTAATG GCTCCCCATT TGCTAATAGC 
GATGCCTGCT TTGCCCTGGC CTATGCTGTT ATCATGCTTA ACACTGACCA ACATAACCAC 
AATGTTCGCA AGCAAAATGC ACCCATGACC CTGGAGGAGT TCCGCAAAAA CCTGAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAAG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAGGAACAA ACAGGTCTGG TTCGTGAGAA TTATGTGTGG 
AGTGTGCTGC TTCATCGAGG TGCTACCCCT GAGGGCATCT TCCTTCGTGT ACCCCCTGGC 
AGCTATGATC TTGACCTCTT CACTATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT 
GTGTTCGATA AAAGCCTCGA GGAGACCATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGTGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT 
CTGTGCAAAT TCACAGCTCT AAGTAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTCGGA 
AGCAACCCTA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GACATCCTTC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTTTTAC CGAAGGCTAT GGTAGAGGTA GAAGATTTTG TGGATCCCAA TGGTAAGATC 
TCTCTACAGC GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTCCT TAGTTTTGTA 
AGCTGGCTGA CACTGAGTGG TCCTGAGCAG TCCAGTGTCC GGGGCCCATC GACAGAGAAC 
CAAGAGGCCA AGCGAGTAGC CTTGGACTGC ATCAAGCAAT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTCCA GCTGGAATCT CTGCAGGAGC TCATGAAGGC TCTGGTCTCG 
GTGACACCAG ATGAAGAGAC ATATGATGAA GAAGATGCTG CTTTCTGTCT GGAGATGCTG 
CTAAGGATTG TGTTAGAAAA CAGGGACCGT GTGGGCTGTG TATGGCAGAC TGTTCGAGAT 
CATCTATACC ACCTGTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGAGCAGTG 
GTAGGGCTGC TACGCCTAGC CATTCGGCTA CTCCGAAGAG AAGAGATTAG TGGCCAGGTC 
CTGCTCTCTC TGCGCATCTT GTTACTGATG AAGCCCAGCG TGCTGTCCAG AGTCAGCCAC 
CAGGTCGCTT ATGGGCTGCA TGAACTCCTC AAGACCAATG CTGCCAACAT CCACTCGGGT 
GATGACTGGG CCACTCTCTT CACATTGCTG GAGTGCATTG GCTCAGGCGT GAAGCCTCCA 
GATGCTCTAC AAGCCACAGC CAGGGCTGAT GCTCCTGATG CTGGAGCACA ATCCGACAGT 
GAGCTCCCAT CCTACCATCA AAATGATGTC AGCCTAGACC GAGGGTACAC TTCCGACTCA 
GAAGTCTACA CTGACCATGG CAGGCCTGGC AAGATGCACC GATCAGCCAC AGACGCTGAC 
ATGGCCAACA GTGGTTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAACCAGCA 
GCAGGACTCA GCAGGCCAGG CCCTTCGCCC TTGGTCAATC AGTACAGCCT TACAGTGGGC 
CTGGACCTGG GACCACATGA CACTAAGTCC TTGCTTAAGT GTGTGGAGTC TCTGTCCTTC 
ATTGTTCGTG ATGCTGCTCA CATCACCCCT GACAACTTTG AACTCTGCGT CAAGACCCTC 
CGCATCTTTG TAGAAGCCAG TCTGAATGGT GGGTGCAAGT CCCAGGATAA ACGTGGCAAG 
AGTCATAAAT ATGACAGCAA AGGGAACCGG TTCAAGAAAA AGCCCAAGGA GGGCTCGATG 
CTTCGGCGGC CCCGGGCATC CAGCCAGCAT GCCACTCGGG CTGGACACAG TGATGAAGAG 
GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG 
CACACCCTGC ACACACGGGC AGCCTCTATC TACAGTTCAT GGGCAGAGGA GCAACGCCAC 
CTGGAGACAG GTGGCCGGAA GATTGAAGCT GACTCACGTA CCCTCTGGGC CCACTGCTGG 
TGCCCTTTAT TACAAGGTAT CGCTTGCTTA TGCTGTGACG CCCGGCGCCA GGTGCGGATG 
CAGGCCCTGA CATATCTCCA GCGAGCCCTT CTGGTACATG ACCTGCAAAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCGC TACTGACCAA GCTTTTAGAA 
AATATCAGCC CTGCGGATGT GGGCGGGATG GAGGAGACCC GGATGCGGGC TTCCACACTG 
CTCTCAAAGG TCTTCCTGCA GCACCTGTCC CCACTGCTGT CCCTGTCCAC CTTTGCTGCC 
CTGTGGCTCA CCATCCTGGA CTTCATGGAC AAGTACATGC ACGCAGGTTC CAGTGACTTG 
CTGTCAGAAG CTGTCCCTGA GTCTCTGAAG AACATGCTCC TTGTGATGGA CACAGCAGAG 
ATTTTCCACA GTGCAGATGC AAGGGGAGGC AGCCCCTCTG CTCTCTGGGA GATCACCTGG 
GAGCGCATCG ATTGCTTCTT ACCACACCTA CGTGATGAAC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCCACAGA GCCTCACAGT CAAAAACCTC TGGCTTCTAC TCACCTGACG 
CCTGCTGCTG GTGACCCCAG GATGCCTGGC CATCCACCTC CTCCAGAGAT ACCCTCAGAA 
GTGGGAGCTT GTGACCCGGA GAAGCCCGAG AGCACCAGAG CTGCCAGCAG CAGCTCCCCT 
GGATCGCCGG TGGCCTCTAG CCCCAGCAAA CAGAGTCCTG CCCCAGAGGG ACCTCCCCCA 
CTGGCCCAGC CCCCACTAAT CCTACAGCCC TTGACCTCCC CACTGCAGGT GGGGGTACCA 
CCCATGACTT TGCCCATCAT CCTCAACCCT GCACTCATTG AGGCCACCTC TCCAGTGCCT 
CTCTTAGCCA CGCCACGCCC TACAGACCCC ATCCCCACCT CTGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M.
Proceedings of the National Academy of Sciences of the United States of America114(17)E3462-E3471(2017 Apr)
Hansen MD,Johnsen IB,Stiberg KA,Sherstova T,Wakita T,Richard GM,Kandasamy RK,Meurs EF,Anthonsen MW

The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).
The Journal of biological chemistry288(16)11532-45(2013 Apr)
Lowery J,Szul T,Styers M,Holloway Z,Oorschot V,Klumperman J,Sztul E

AMPK phosphorylates GBF1 for mitotic Golgi disassembly.
Journal of cell science126(Pt 6)1498-505(2013 Mar)
Mao L,Li N,Guo Y,Xu X,Gao L,Xu Y,Zhou L,Liu W

GBF1 bears a novel phosphatidylinositol-phosphate binding module, BP3K, to link PI3K? activity with Arf1 activation involved in GPCR-mediated neutrophil chemotaxis and superoxide production.
Molecular biology of the cell23(13)2457-67(2012 Jul)
Mazaki Y,Nishimura Y,Sabe H

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Gbf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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