GBF1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005637661.3	XP_005637718.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
XM_543987.5	XP_543987.2	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
XM_014108957.1	XP_013964432.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
XM_005637657.3	XP_005637714.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
XM_005637658.3	XP_005637715.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_005637661.2	XP_005637718.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
XM_014108958.2	XP_013964433.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
XM_005637660.3	XP_005637717.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
XM_543987.6	XP_543987.2	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
XM_005637657.2	XP_005637714.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
XM_005637658.2	XP_005637715.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_005637659.2	XP_005637716.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
XM_005637660.2	XP_005637717.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
XM_014108958.1	XP_013964433.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
XM_005637659.3	XP_005637716.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
XM_014108957.2	XP_013964432.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe43951	XM_014108957.2 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43952	XM_014108958.2 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe27947	XM_005637657.3 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27948	XM_005637658.3 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27949	XM_005637659.3 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27950	XM_005637660.3 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27951	XM_005637661.3 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27952	XM_543987.6 Latest version!	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe27951	XM_005637661.2	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27952	XM_543987.5	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27947	XM_005637657.2	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27948	XM_005637658.2	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe27950	XM_005637660.2	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe43952	XM_014108958.1	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe27949	XM_005637659.2	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe43951	XM_014108957.1	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

GBF1 ( XM_014108957.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964432.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe43951
Clone ID Related Accession (Same CDS sequence)	XM_014108957.1 , XM_014108957.2
Accession Version	XM_014108957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG 
GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG 
GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC 
CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG 
AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC 
AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG 
GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA 
TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG 
GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG 
GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA 
GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG 
ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC 
AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT 
GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG 
GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964432.1
CDS	251..5836
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_014108957.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108957.1

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG 
GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG 
GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC 
CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG 
AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC 
AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG 
GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA 
TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG 
GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG 
GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA 
GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG 
ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC 
AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT 
GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG 
GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_014108957.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964432.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

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CloneID	OCe43951
Clone ID Related Accession (Same CDS sequence)	XM_014108957.1 , XM_014108957.2
Accession Version	XM_014108957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG 
GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG 
GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC 
CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG 
AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC 
AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG 
GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA 
TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG 
GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG 
GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA 
GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG 
ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC 
AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT 
GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG 
GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964432.1
CDS	269..5854
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_014108957.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108957.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG 
TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG 
GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG 
GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC 
CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG 
AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC 
AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG 
GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT 
ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA 
TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG 
GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG 
GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG 
CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA 
GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG 
ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC 
AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT 
GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG 
GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT 
TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_014108958.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964433.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

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CloneID	OCe43952
Clone ID Related Accession (Same CDS sequence)	XM_014108958.2 , XM_014108958.1
Accession Version	XM_014108958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG 
CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC 
CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG 
TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG 
CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC 
CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG 
CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA 
CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG 
ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG 
GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT 
CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG 
CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT 
GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT 
CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA 
CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964433.1
CDS	269..5842
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_014108958.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108958.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG 
CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC 
CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG 
TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG 
CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC 
CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG 
CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA 
CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG 
ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG 
GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT 
CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG 
CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT 
GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT 
CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA 
CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_014108958.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964433.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

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CloneID	OCe43952
Clone ID Related Accession (Same CDS sequence)	XM_014108958.2 , XM_014108958.1
Accession Version	XM_014108958.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG 
CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC 
CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG 
TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG 
CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC 
CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG 
CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA 
CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG 
ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG 
GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT 
CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG 
CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT 
GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT 
CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA 
CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964433.1
CDS	251..5824
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_014108958.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108958.1

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC 
CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC 
ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC 
CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT 
CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC 
CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT 
GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC 
TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC 
TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA 
AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG 
GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC 
AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC 
AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC 
CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT 
ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA 
GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC 
ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA 
TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA 
CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG 
AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT 
CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC 
CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC 
ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA 
GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG 
TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC 
GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC 
AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT 
GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG 
AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG 
AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC 
AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT 
GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG 
TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT 
CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA 
AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT 
GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC 
CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC 
TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG 
AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC 
CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC 
ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA 
GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG 
CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC 
CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG 
GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG 
CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC 
CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT 
GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA 
GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC 
GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG 
GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT 
GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT 
CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG 
CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC 
ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC 
CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG 
AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG 
CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT 
GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG 
CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC 
CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG 
TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG 
CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC 
CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG 
AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG 
CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA 
CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG 
ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG 
GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC 
CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT 
CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG 
CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT 
GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT 
CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA 
CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC 
CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637657.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637714.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe27947
Clone ID Related Accession (Same CDS sequence)	XM_005637657.3 , XM_005637657.2
Accession Version	XM_005637657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG 
CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA 
GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA 
CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG 
ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC 
CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG 
GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG 
ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG 
GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC 
AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT 
GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT 
GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC 
TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA 
GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG 
CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT 
CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC 
ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637714.1
CDS	269..5953
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637657.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637657.3

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG 
CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA 
GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA 
CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG 
ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC 
CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG 
GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG 
ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG 
GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC 
AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT 
GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT 
GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC 
TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA 
GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG 
CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT 
CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC 
ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637657.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637714.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

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CloneID	OCe27947
Clone ID Related Accession (Same CDS sequence)	XM_005637657.3 , XM_005637657.2
Accession Version	XM_005637657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG 
CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA 
GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA 
CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG 
ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC 
CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG 
GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG 
ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG 
GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC 
AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT 
GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT 
GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC 
TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA 
GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG 
CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT 
CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC 
ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637714.1
CDS	251..5935
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637657.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637657.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG 
CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA 
GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA 
CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG 
ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG 
GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC 
CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG 
GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG 
ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG 
GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC 
AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT 
GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC 
ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT 
GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC 
TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA 
GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG 
CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT 
CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC 
ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637658.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637715.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

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CloneID	OCe27948
Clone ID Related Accession (Same CDS sequence)	XM_005637658.3 , XM_005637658.2
Accession Version	XM_005637658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA 
TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC 
TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC 
TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC 
CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC 
ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC 
CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG 
CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG 
GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG 
GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC 
TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT 
TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT 
CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT 
GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA 
GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA 
GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT 
GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT 
GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA 
GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG 
CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC 
ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG 
AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG 
AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC 
TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT 
TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT 
GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC 
ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG 
CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA 
GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC 
ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC 
ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA 
ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT 
GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC 
AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA 
GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG 
AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG 
GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA 
TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT 
GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG 
GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG 
GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT 
GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG 
GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG 
GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT 
CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA 
CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA 
CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA 
CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG 
GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT 
GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG 
CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG 
GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT 
TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT 
AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT 
ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA 
AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC 
CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC 
ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT 
GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG 
CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT 
TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC 
ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT 
GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC 
TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG 
CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA 
GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT 
GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG 
GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA 
CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637715.1
CDS	269..5950
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637658.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637658.3

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA 
TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC 
TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC 
TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC 
CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC 
ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC 
CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG 
CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG 
GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG 
GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC 
TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT 
TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT 
CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT 
GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA 
GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA 
GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT 
GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT 
GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA 
GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG 
CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC 
ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG 
AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG 
AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC 
TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT 
TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT 
GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC 
ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG 
CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA 
GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC 
ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC 
ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA 
ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT 
GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC 
AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA 
GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG 
AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG 
GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA 
TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT 
GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG 
GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG 
GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT 
GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG 
GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG 
GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT 
CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA 
CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA 
CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA 
CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG 
GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT 
GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG 
CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG 
GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT 
TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT 
AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT 
ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA 
AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC 
CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC 
ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT 
GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG 
CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT 
TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC 
ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT 
GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC 
TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG 
CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA 
GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT 
GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG 
GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA 
CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637658.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637715.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

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CloneID	OCe27948
Clone ID Related Accession (Same CDS sequence)	XM_005637658.3 , XM_005637658.2
Accession Version	XM_005637658.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA 
TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC 
TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC 
TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC 
CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC 
ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC 
CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG 
CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG 
GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG 
GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC 
TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT 
TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT 
CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT 
GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA 
GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA 
GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT 
GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT 
GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA 
GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG 
CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC 
ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG 
AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG 
AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC 
TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT 
TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT 
GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC 
ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG 
CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA 
GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC 
ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC 
ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA 
ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT 
GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC 
AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA 
GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG 
AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG 
GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA 
TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT 
GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG 
GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG 
GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT 
GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG 
GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG 
GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT 
CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA 
CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA 
CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA 
CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG 
GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT 
GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG 
CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG 
GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT 
TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT 
AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT 
ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA 
AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC 
CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC 
ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT 
GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG 
CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT 
TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC 
ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT 
GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC 
TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG 
CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA 
GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT 
GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG 
GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA 
CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637715.1
CDS	251..5932
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637658.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637658.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA 
TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC 
TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC 
TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC 
CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC 
ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC 
CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG 
CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG 
GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG 
GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC 
TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT 
TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT 
CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT 
GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA 
GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA 
GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT 
GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT 
GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA 
GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG 
CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC 
ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG 
AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG 
AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC 
TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT 
TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT 
GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC 
ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG 
CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA 
GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC 
ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC 
ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA 
ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT 
GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC 
AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA 
GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG 
AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG 
GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA 
TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT 
GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG 
GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG 
GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT 
GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG 
GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG 
GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT 
CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA 
CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA 
CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA 
CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG 
GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT 
GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG 
CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG 
GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT 
TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT 
AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT 
ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG 
AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA 
AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC 
CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC 
TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC 
ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT 
GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG 
CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT 
TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG 
TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT 
GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC 
TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC 
ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT 
GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC 
TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG 
CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA 
GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT 
GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG 
GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT 
CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA 
CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA 
CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637659.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637716.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

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CloneID	OCe27949
Clone ID Related Accession (Same CDS sequence)	XM_005637659.2 , XM_005637659.3
Accession Version	XM_005637659.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA 
GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG 
ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT 
GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA 
CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT 
AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG 
GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG 
CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC 
TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG 
TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC 
CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG 
CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG 
CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG 
ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG 
AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC 
CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC 
CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC 
CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC 
ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637716.1
CDS	251..5923
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637659.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637659.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA 
GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG 
ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT 
GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA 
CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT 
AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG 
GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG 
CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC 
TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG 
TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC 
CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG 
CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG 
CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG 
ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG 
AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC 
CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC 
CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC 
CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC 
ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637659.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637716.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

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CloneID	OCe27949
Clone ID Related Accession (Same CDS sequence)	XM_005637659.2 , XM_005637659.3
Accession Version	XM_005637659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA 
GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG 
ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT 
GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA 
CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT 
AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG 
GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG 
CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC 
TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG 
TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC 
CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG 
CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG 
CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG 
ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG 
AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC 
CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC 
CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC 
CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC 
ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637716.1
CDS	269..5941
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERVLLFKNLKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637659.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637659.3

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC 
TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG 
AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG 
TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT 
GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC 
TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC 
CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA 
GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA 
GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT 
GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG 
TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC 
TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC 
CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA 
ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA 
GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG 
AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT 
GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG 
CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC 
TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT 
GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC 
ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA 
GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA 
GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA 
GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT 
GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG 
CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA 
GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC 
ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC 
AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT 
GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA 
GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG 
CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC 
TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA 
CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT 
GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT 
GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT 
GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC 
ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG 
GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC 
TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT 
AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT 
GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG 
AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG 
GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA 
CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT 
ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC 
TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG 
CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA 
TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC 
AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC 
CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC 
ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG 
CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT 
GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC 
ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC 
AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG 
AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC 
AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA 
GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC 
CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC 
ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC 
ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT 
CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA 
GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT 
GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC 
AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA 
GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG 
ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT 
GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA 
CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT 
AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG 
GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG 
CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC 
TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG 
TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC 
CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG 
CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG 
CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG 
ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG 
AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC 
CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC 
CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC 
CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC 
ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637660.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637717.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

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CloneID	OCe27950
Clone ID Related Accession (Same CDS sequence)	XM_005637660.3 , XM_005637660.2
Accession Version	XM_005637660.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG 
AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC 
CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC 
TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT 
GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC 
TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG 
TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG 
CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA 
GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG 
GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT 
TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC 
AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA 
GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT 
GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT 
TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG 
GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT 
GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC 
ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC 
ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC 
CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA 
GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG 
GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA 
GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA 
CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG 
TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA 
ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG 
AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG 
GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG 
AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT 
CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA 
CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC 
CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT 
GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC 
AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG 
TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG 
GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT 
CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT 
GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC 
TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC 
AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG 
CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG 
GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG 
CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT 
CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA 
GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC 
CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC 
CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG 
AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC 
TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG 
CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT 
GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG 
ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG 
TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC 
AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA 
GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG 
GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA 
TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA 
GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT 
AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC 
AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA 
GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC 
TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC 
AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG 
CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC 
AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT 
TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC 
TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG 
GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG 
TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC 
ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC 
CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA 
GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA 
AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT 
CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC 
CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG 
AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT 
CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG 
CCCACCTCCG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637717.1
CDS	269..5929
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637660.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637660.3

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG 
AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC 
CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC 
TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT 
GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC 
TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG 
TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG 
CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA 
GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG 
GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT 
TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC 
AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA 
GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT 
GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT 
TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG 
GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT 
GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC 
ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC 
ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC 
CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA 
GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG 
GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA 
GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA 
CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG 
TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA 
ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG 
AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG 
GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG 
AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT 
CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA 
CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC 
CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT 
GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC 
AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG 
TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG 
GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT 
CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT 
GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC 
TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC 
AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG 
CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG 
GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG 
CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT 
CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA 
GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC 
CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC 
CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG 
AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC 
TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG 
CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT 
GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG 
ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG 
TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC 
AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA 
GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG 
GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA 
TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA 
GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT 
AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC 
AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA 
GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC 
TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC 
AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG 
CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC 
AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT 
TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC 
TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG 
GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG 
TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC 
ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC 
CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA 
GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA 
AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT 
CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC 
CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG 
AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT 
CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG 
CCCACCTCCG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637660.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637717.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

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CloneID	OCe27950
Clone ID Related Accession (Same CDS sequence)	XM_005637660.3 , XM_005637660.2
Accession Version	XM_005637660.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG 
AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC 
CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC 
TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT 
GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC 
TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG 
TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG 
CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA 
GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG 
GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT 
TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC 
AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA 
GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT 
GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT 
TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG 
GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT 
GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC 
ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC 
ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC 
CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA 
GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG 
GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA 
GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA 
CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG 
TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA 
ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG 
AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG 
GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG 
AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT 
CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA 
CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC 
CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT 
GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC 
AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG 
TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG 
GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT 
CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT 
GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC 
TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC 
AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG 
CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG 
GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG 
CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT 
CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA 
GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC 
CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC 
CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG 
AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC 
TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG 
CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT 
GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG 
ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG 
TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC 
AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA 
GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG 
GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA 
TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA 
GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT 
AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC 
AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA 
GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC 
TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC 
AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG 
CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC 
AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT 
TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC 
TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG 
GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG 
TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC 
ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC 
CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA 
GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA 
AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT 
CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC 
CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG 
AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT 
CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG 
CCCACCTCCG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637717.1
CDS	251..5911
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKISPCLNKLELSSGEQTKALNQLERLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637660.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637660.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA 
CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG 
AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC 
CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC 
TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT 
GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC 
TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG 
TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG 
CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA 
GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG 
GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT 
TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC 
AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA 
GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT 
GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT 
TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG 
GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT 
GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC 
ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC 
ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC 
CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA 
GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG 
GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA 
GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA 
CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG 
TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA 
ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG 
AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG 
GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG 
AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT 
CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA 
CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC 
CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT 
GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC 
AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG 
TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG 
GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT 
CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT 
GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC 
TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC 
AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG 
CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG 
GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG 
CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT 
CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA 
GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC 
CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC 
CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG 
AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC 
TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG 
CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT 
GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG 
ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG 
TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC 
AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA 
GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG 
GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA 
TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA 
GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT 
AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC 
AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA 
GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC 
TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG 
GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC 
AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG 
CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG 
GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC 
AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT 
TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC 
TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG 
GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG 
TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG 
GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA 
CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG 
TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC 
ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC 
CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA 
GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA 
AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT 
CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC 
CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG 
AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG 
GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT 
CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG 
CCCACCTCCG AGGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637661.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637718.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

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CloneID	OCe27951
Clone ID Related Accession (Same CDS sequence)	XM_005637661.3 , XM_005637661.2
Accession Version	XM_005637661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG 
GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC 
TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG 
CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG 
CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG 
ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG 
AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC 
TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA 
GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG 
ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC 
TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC 
TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA 
GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC 
AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA 
GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG 
CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC 
ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637718.1
CDS	269..5857
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637661.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637661.3

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG 
GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC 
TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG 
CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG 
CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG 
ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG 
AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC 
TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA 
GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG 
ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC 
TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC 
TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA 
GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC 
AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA 
GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG 
CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC 
ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_005637661.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637718.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

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CloneID	OCe27951
Clone ID Related Accession (Same CDS sequence)	XM_005637661.3 , XM_005637661.2
Accession Version	XM_005637661.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG 
GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC 
TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG 
CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG 
CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG 
ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG 
AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC 
TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA 
GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG 
ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC 
TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC 
TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA 
GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC 
AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA 
GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG 
CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC 
ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637718.1
CDS	251..5839
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_005637661.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637661.2

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC 
TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG 
GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC 
TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG 
CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG 
CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG 
ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG 
AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC 
TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA 
GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG 
ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC 
TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC 
AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC 
TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA 
GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC 
AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA 
GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG 
CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC 
ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_543987.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543987.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

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CloneID	OCe27952
Clone ID Related Accession (Same CDS sequence)	XM_543987.5 , XM_543987.6
Accession Version	XM_543987.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543987.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG 
ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC 
CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC 
TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG 
ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT 
GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG 
GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA 
CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG 
GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC 
TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA 
GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC 
TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG 
ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT 
GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT 
CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT 
CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA 
CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG 
CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543987.2
CDS	251..5827
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_543987.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543987.5

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG 
ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC 
CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC 
TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG 
ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT 
GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG 
GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA 
CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG 
GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC 
TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA 
GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC 
TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG 
ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT 
GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT 
CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT 
CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA 
CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG 
CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_543987.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543987.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

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CloneID	OCe27952
Clone ID Related Accession (Same CDS sequence)	XM_543987.5 , XM_543987.6
Accession Version	XM_543987.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543987.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG 
ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC 
CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC 
TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG 
ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT 
GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG 
GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA 
CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG 
GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC 
TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA 
GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC 
TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG 
ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT 
GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT 
CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT 
CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA 
CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG 
CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543987.2
CDS	269..5845
Translation	MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFSNLKEVLNNITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPAGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYMGTNMKKLKMRAGGMSDSSKWKKQKRSPRPLRHMTKVTSGSELPTSNGTTLSSNLTGGMPFIDVPTPISSASSEAASTVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDPQQEGVHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLLQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWHIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKEAARSGYEAVDGTRESSNSERAASDGKPVGIAADIPGLHLPGGGRLPAEHGKPGCSDLEEAADSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRNCNGSPFANSDACFALAYAVILLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPAGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGTEQSSVRGPSTENQEAKRAALDCIKHCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARAEAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYADHCRPGKIHRSATDADVVNSGWLVVGKDDIDNSKPGPGPSRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSVLRRPRTSGQHATRGGHSDDDEDEGVPASYHTVSLQLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPLEPHAQKTLASAHMTPAAGDTRTPGHPPPPEMPSELGACDFEKPEGPRPANSSSPGSPVASSPNRLSPTSDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPMPTSEVN

Target ORF information:

RefSeq Version	XM_543987.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543987.6

ORF Insert Sequence:

ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG 
CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA 
CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT 
ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC 
ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT 
TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT 
CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT 
ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC 
ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG 
ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC 
CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA 
GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC 
ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT 
ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA 
GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG 
TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT 
CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT 
GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC 
CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA 
TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC 
CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA 
TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC 
AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG 
ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT 
ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG 
TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT 
GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT 
CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC 
CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA 
GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA 
GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG 
CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA 
AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG 
TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG 
CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA 
AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT 
GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG 
AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT 
AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC 
CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA 
GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC 
AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG 
TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT 
GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT 
TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG 
AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA 
TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT 
GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT 
GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA 
GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG 
ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT 
GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG 
AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG 
ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC 
TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG 
CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA 
GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA 
GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG 
GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT 
CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA 
GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT 
CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC 
AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC 
TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT 
GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA 
GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG 
GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC 
TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT 
CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC 
AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA 
GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT 
GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG 
ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC 
CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC 
TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG 
ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT 
GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG 
GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA 
CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG 
GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC 
TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA 
GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC 
TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC 
ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG 
ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT 
GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT 
CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT 
CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA 
CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG 
CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GBF1
Entrez Gene ID	486857
Full Name	golgi brefeldin A resistant guanine nucleotide exchange factor 1
General protein information	Preferred Names golgi brefeldin A resistant guanine nucleotide exchange factor 1 Names Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 golgi-specific brefeldin A resistance factor 1 golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GBF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

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