gbf-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gbf-1
Entrez Gene ID	176609
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001268210.2	NP_001255139.1	SEC7 domain-containing protein
NM_001268211.1	NP_001255140.1	hypothetical protein
NM_001268210.1	NP_001255139.1	hypothetical protein
NM_001268211.2	NP_001255140.1	SEC7 domain-containing protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GBF1	XP_543987.2
Rattus norvegicus (Norway rat)	Gbf1	NP_001178563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001527	XP_321598.5
Arabidopsis thaliana (thale cress)	GN	NP_001184991.1
Xenopus tropicalis (tropical clawed frog)	gbf1	XP_004915993.1
Homo sapiens (human)	GBF1	NP_001186308.1
	GBF1	XP_001104407.2
Mus musculus (house mouse)	Gbf1	NP_849261.2
Gallus gallus (chicken)	GBF1	XP_421632.4
Drosophila melanogaster (fruit fly)	garz	NP_001246278.1
Caenorhabditis elegans (roundworm)	gbf-1	NP_001255140.1
Pan troglodytes (chimpanzee)	GBF1	XP_521592.3
Danio rerio (zebrafish)	si:dkey-183c23.1	XP_694714.5
Oryza sativa (rice)	Os03g0666100	NP_001050844.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gbf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa352774	NM_001268210.2 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCa352773	NM_001268211.2 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa352774	NM_001268210.1	Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OCa352773	NM_001268211.1	Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gbf-1 ( NM_001268210.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255139.1 Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), partial mRNA.

Price & Availability↑

CloneID	OCa352774
Clone ID Related Accession (Same CDS sequence)	NM_001268210.2 , NM_001268210.1
Accession Version	NM_001268210.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268210.1. COMPLETENESS: incomplete on the 5' end.

ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC CAACTCCATT CTCAAACTCC GGCAATCTTT 
GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT GCATCGGATC TGGCGACTGT CGCGTTCATA 
TGGACAGATA TCTGGCGCCC GTTGCTCCAA GCAATTGGAC GGCTCAGCTG TGATTGCCGA 
CGAGGTGTCC GTGCGGCGGC GTTGACTCAT TTGCAGCGCG CCTTCCTGCC GGCAAATATG 
GCAACTCTTG GAGCCGCAGA ATGGCAATCA TGCTTTGGAG AAGTGCTCTT CCCATTGCTC 
ACTAAGCTTC TGGAGCCATT CTCTCAAATG GATCCGATTG GAATGGAGGA TACACGTGTT 
CGGACACTTC AAATCGTCGC GAAAACTCTT TTAAATCATC TCTCAGCCCT GTCGGCTCTT 
GATTCATTCC CGGATCTTTG GATGCTTCTG CTCGACTATA TGGAGCAATA TCTTCGAGTT 
GATAGCTGTG GAAATTTGAA TGAAGCAGTG CCGGAATCGC TGAAAAATAT GCTTCTCGTT 
ATGGATAGTA CGGGAATTTT CGCTGCAACA CCTCGGCTTT ATGATGTGAC TGTGGAGAGG 
CTCAACAAAT TTATGCCCGA ATTGATTAAG GATACGATTC CGAATCCACC ACGGCCAGGG 
CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG AAGGAGCATG CTTCTGGGCT TGAGCCACCA 
CCACCATCAT CCAACTCAAC AGCCGCCACT TCAACATCTG ATCCTTCGAT CGCTACTGCA 
CAATCTTCAA TTTCGACAGC TTCATCTGTT GTTGGACCAC TTGTTACATG TCCAGAAGAT 
GCTGGAATTA GTGCTCCGAT TCCAATACAA CATCCATTGA CTGAAGTAAT TGTTCACTCG 
GGACCAACAT CACCAATTGG CTCACCACCA CAAACTGAGC CACCAGCTTC AAGTCCCCCG 
CAACACCAAC ATTCGGAGCA CCAACAATAT GAGCAATATC GACAGCAGCA GGCAGCGGCT 
GCACAGCAAT ATCAGCAGTA TAATCAGAAC TATCCCCAAC AACAGCAGCA ACAGCAGCAA 
CAATATGCAT ATTCACCAGA ACACGCGGCA TACTATCAGC AACAATATGC ACACCAACAA 
CAGCAATATG CAGAGCATTA TGCCAATCAA TATCAGCACT ATCAACAGCA GCAACAGCAA 
CAACAGCAGC ACCCAGTAAA TCCAACGAGT CCCAGTGTTC ACGGACAGTA TTCGGTGGCG 
AATCCTCTTC CACTGCCGGC TCATCCAGCT TATCATCCGA TTGTTGCACC ATCAGTGAAC 
AGTGCATTCA CCCATGTGTA CACTCCGCCA CAAAATAATG CTCCTGCTCT TGCTCCATCT 
GCTCCAACAA CAACGTCAGC AGATTCTCCG TATTTCACCC CGATTCCCTA CAATCCGAGC 
CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255139.1
CDS	1..1458
Translation	MSLHLLDLCSQLHSQTPAIFAKWAQGASPAASDLATVAFIWTDIWRPLLQAIGRLSCDCRRGVRAAALTHLQRAFLPANMATLGAAEWQSCFGEVLFPLLTKLLEPFSQMDPIGMEDTRVRTLQIVAKTLLNHLSALSALDSFPDLWMLLLDYMEQYLRVDSCGNLNEAVPESLKNMLLVMDSTGIFAATPRLYDVTVERLNKFMPELIKDTIPNPPRPGQQQSEASEPKKEHASGLEPPPPSSNSTAATSTSDPSIATAQSSISTASSVVGPLVTCPEDAGISAPIPIQHPLTEVIVHSGPTSPIGSPPQTEPPASSPPQHQHSEHQQYEQYRQQQAAAAQQYQQYNQNYPQQQQQQQQQYAYSPEHAAYYQQQYAHQQQQYAEHYANQYQHYQQQQQQQQQHPVNPTSPSVHGQYSVANPLPLPAHPAYHPIVAPSVNSAFTHVYTPPQNNAPALAPSAPTTTSADSPYFTPIPYNPSQQEKP

Target ORF information:

RefSeq Version	NM_001268210.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268210.2

ORF Insert Sequence:

ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC CAACTCCATT CTCAAACTCC GGCAATCTTT 
GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT GCATCGGATC TGGCGACTGT CGCGTTCATA 
TGGACAGATA TCTGGCGCCC GTTGCTCCAA GCAATTGGAC GGCTCAGCTG TGATTGCCGA 
CGAGGTGTCC GTGCGGCGGC GTTGACTCAT TTGCAGCGCG CCTTCCTGCC GGCAAATATG 
GCAACTCTTG GAGCCGCAGA ATGGCAATCA TGCTTTGGAG AAGTGCTCTT CCCATTGCTC 
ACTAAGCTTC TGGAGCCATT CTCTCAAATG GATCCGATTG GAATGGAGGA TACACGTGTT 
CGGACACTTC AAATCGTCGC GAAAACTCTT TTAAATCATC TCTCAGCCCT GTCGGCTCTT 
GATTCATTCC CGGATCTTTG GATGCTTCTG CTCGACTATA TGGAGCAATA TCTTCGAGTT 
GATAGCTGTG GAAATTTGAA TGAAGCAGTG CCGGAATCGC TGAAAAATAT GCTTCTCGTT 
ATGGATAGTA CGGGAATTTT CGCTGCAACA CCTCGGCTTT ATGATGTGAC TGTGGAGAGG 
CTCAACAAAT TTATGCCCGA ATTGATTAAG GATACGATTC CGAATCCACC ACGGCCAGGG 
CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG AAGGAGCATG CTTCTGGGCT TGAGCCACCA 
CCACCATCAT CCAACTCAAC AGCCGCCACT TCAACATCTG ATCCTTCGAT CGCTACTGCA 
CAATCTTCAA TTTCGACAGC TTCATCTGTT GTTGGACCAC TTGTTACATG TCCAGAAGAT 
GCTGGAATTA GTGCTCCGAT TCCAATACAA CATCCATTGA CTGAAGTAAT TGTTCACTCG 
GGACCAACAT CACCAATTGG CTCACCACCA CAAACTGAGC CACCAGCTTC AAGTCCCCCG 
CAACACCAAC ATTCGGAGCA CCAACAATAT GAGCAATATC GACAGCAGCA GGCAGCGGCT 
GCACAGCAAT ATCAGCAGTA TAATCAGAAC TATCCCCAAC AACAGCAGCA ACAGCAGCAA 
CAATATGCAT ATTCACCAGA ACACGCGGCA TACTATCAGC AACAATATGC ACACCAACAA 
CAGCAATATG CAGAGCATTA TGCCAATCAA TATCAGCACT ATCAACAGCA GCAACAGCAA 
CAACAGCAGC ACCCAGTAAA TCCAACGAGT CCCAGTGTTC ACGGACAGTA TTCGGTGGCG 
AATCCTCTTC CACTGCCGGC TCATCCAGCT TATCATCCGA TTGTTGCACC ATCAGTGAAC 
AGTGCATTCA CCCATGTGTA CACTCCGCCA CAAAATAATG CTCCTGCTCT TGCTCCATCT 
GCTCCAACAA CAACGTCAGC AGATTCTCCG TATTTCACCC CGATTCCCTA CAATCCGAGC 
CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbf-1 ( NM_001268211.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255140.1 Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), mRNA.

Price & Availability↑

CloneID	OCa352773
Clone ID Related Accession (Same CDS sequence)	NM_001268211.1 , NM_001268211.2
Accession Version	NM_001268211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268211.1.

ATGGCGACCA ACGGCGTCTA CATCGTGATG GGCGAGGCGA ATTGTGTCGT CGCGCTGCTC 
AATAAGGCTC GGAGACAATA CCAACTGTCA CAAGTGCCGA CGCTCGAAGA CACGGATCCA 
CTGCTGCGAA ACTTCACAGA TCTCAAGGAA GTGCTGAATG AGGTTGCCGA TCTGGCAGAT 
ATGAATCCAC AAACGTATCT ATCACCGTTT CTCGACGTAA TAAAGGCTCA GAACACGAAT 
GGACCGATTA CGGAAGCCGC GCTGGCCGCT GTCGCCAAAT TTCTGAACTA CGGGCTCATT 
GACGCGAGCA GTATTAAGGC GGCGAACGCT GTCGAGTCTA TCGCCTACGC TGTGGTTCAT 
ACAAAGTTTA TCGGCGGGAA AAGCACTGGA AGTGATGAAT GTGTCCTTTT TAAGATTCTA 
CAGGTGCTCC GCTCGCTGCT TCTCTCCCCT CCAGGCATCC TTTTGTCTAA TGAAGCCGTC 
TGTGATATGA TGCAGTCTTG TTTCAGAATT GTGTTCGAGC AGAACCTCTC TCTGCTTCTT 
AGAAAGGCCG CCGAATCGAC GCTAGCCGAC ATGACACAGC TCATTTTCAC CAGATTGCCA 
ACTTTTGTCG AAGACACCCG TCATCCATAC ATTCGGCAGC TGGTAAATCC CACTGAAAAA 
CGACAAAAAC GGAAGAAAAA GCGTCAATTG TCAGTGCATA TTGAGACGAA GGCCAAGGAA 
CCGGAGAATG TGCCAACGGA AATGACAAAA TTGATTGGAG AAGCTGCAGA AACTGCTGAA 
ACTGACGGAG CAGCGAATCT TGGCTACGAC GTAGTGCTCA CGACGGATCC ACCAGTCGAT 
ACAGTAACCC ACCCGGATCC ACCAATTGAA GAAATCATAA AGCTTGCTGA GCCAATTTCA 
GCCGGAGATG AAGCCGATTC AGAATCAGAA GGCGGTGGCG GTGAAGAGCA TCACGAACGT 
CCCCCCGTTC GGGCTCACGC TGGATTACAG AGAGAAATTG TGTCGGATGA AGAGGAAATC 
GATACGGAGC AAACTGTTGG AGGAGAAGAA AAAATGCCCT ATGGGCTCCC ATGTTGTAGA 
GAGCTTTTGA GATTCCTCAT CACAATGACC AACCCAGTTG ACCGTCACAA TACTGAATCA 
ATGGTAATTC TCGGCTTAAA CTTGCTCATT GTTGCTCTGG AAGCCATCGC CGACTTCCTT 
CCAAACTACG ATATTCTCAT GCCACTTATC AAAAATGAGC TCTGTCGAAA TTTGCTCCAA 
CTGCTCGACA CGAATCGCCT ACCAGTGCTC GCCGCCACAA ATCGCTGCTG TTTTTTGCTG 
TTTGAATCGA TGAGAATGCA CATGAAATTC CAGTTGGAAA GCTATTTGAA GAAGCTTCAA 
TCGATTGTGC TCACCGAGGA AAAGCAGCAT GAAAACGGTG GTGGTGGAAC GGAGCAAAAA 
GAAATGGCTT TGGAGTCACT TGTTCAGTTG TGGAGAATTC CCGGATTGGT TACCGAGATG 
TATCTCAATT TTGATTGTGA TCTCTATTGC GGAAATATCT TCGAAGATTT GACAAAGCTT 
CTCGTTGAGA ACTCATTTCC AACAGTTGGT GGACACACAG CCTCGCTGCT CTCTCTGGAT 
GCTCTTTTAG TCGTTATTGA GACTATTGAA CAGAATTGTG AAGATCGAGA GAATGGACGA 
GGAGAAGTTG CCAAAGAGCA AGAGCACAAG GACTTAAAGA AGCTCGGGCT CCCAGTGCTC 
AGCGGCTATG ATCTAGCGAA GAAAATGGCG ATTTCAACGG GAGGAAAAGC TTCTCCAATG 
CCTGTGAGCT CATCAATTGT GCTCCGTTCG AATCGACATG CTCCGTCAAC AGAACTCCCA 
TCGATGAGCC AAATAATTGA ACAAAAGAAG CGAAAACGAC TGATTGCCGA AGGAACCGAA 
CTGTTCAATC AAAGTCCCAA AAAAGGAATC GCTTTTCTCC GGGAAAAGGG AATTCTGGGA 
CACGATGAGC AGAGCCTTGT GCAGTGGCTC CGAACGAATC CACAGTTGGA TAAGAAGGCT 
ATTGCAGATT ATATTTGTAA TCGGAAGCAT GCTGAGGTTC TCAACGCGTT TGTCAAGAGT 
TTTCCATTCG AGAATACCCG CCTGGACGTT GCTCTCCGAA TGTTCCTCGA AACGTTCCGC 
CTGCCTGGAG AATCGGCTGA AATTGCACTT GTAATGCAAC ATTTCTCCGA AGAATGGTTC 
CGAGCCAACA ATGAGCCATT TTTCCATGTC GATGCCGCTT TTACTCTCTC TTATGCGATT 
ATTATGCTCA ATGTTGATCA GCATAATCCG CAGGCCAAAC GAAGTCAACC ACCAATGACA 
GTCGACTGTT TCCGTCGGAA TCTCTCTGGA ACCAACGATT CCCGAGATTT CGACCCAGAA 
ATGCTCGCCG ACATGTACCA GGCCATCAAA ACCGAGGAAA TCGTGATGCC AGCCGAGCAA 
AAGGGCACCG TAAAGGAGGA TTACATGTGG AAAGTGTTGC TCCGTCGTGG TGAGACTGCC 
GAAGGATCGT TTTATCATGC TCCAACCGGA TGGAATGATC ATGATCTATT CGCAGTTTGC 
TGGGGTCCTG CTGTCGCTGC TCTCAGCTAT GTATTCGATA AATCGGAGCA CGAGCAGATA 
CTTCAGAAGG CTCTGACAGG CTATCGGAAG TGTGCGAAAA TCGCTGCCTA CTACGGGATG 
AAGGAGGTTT TCGATAATCT ATGTATTCAT CTTTGCAAGT TCACAACGTT AACTTCAATG 
CGGGATGGTG GAGCTGGTGG TGGAGCCGAT GAGGATGTGG ATCTTTCGGC GGCTGCTCTT 
TTGAGCCATA GCTCCTCACC TGAAGCTGTC GCCCTCGCAT TTGGAGAGAA TCATAAGGCT 
CAACTTGCCA CTCGAACCCT GTTCTATTTG GTTCATGAGA ATGGGAATAT ACTCAGAGAG 
GGATGGCGCA ACTTGTTTGA GGCTCTTCTG CAGCTGTTTA GAGCACGATT GCTTCCTGCA 
GAGCTCACTG AAGTCGAGGA TTATGTTGAT GAGAAGGGAT GGGTTAATAT TCAGAGGGTT 
CATCAGAAAG AGCTCCCACA CACCCGCAAC GATTCAGGCC TTCTTTCATG GTTCGGACTC 
GGTGGAGGCG CTTCAGAAGC GGATCGCCGT AAGCCGACCC AAGAGCAACT GAGCTCGATG 
AAGCTCGCGT CACAAGTCAT TTCGGAGTGC CGTCCGTCGC AAATTGTGGC TGATAGCAAG 
TATCTGACGA GCACATCGCT CGCCGAGCTC CTCTCATCGA TTGCGGCGAA CAGTGCGCAA 
ATTGTTGAGC AAGCCGAGCC ACAGCAAAAG ACCGCAAGCC TGAGTGGAGA GGATGAGGAT 
GCGCTGGTCT TCTACCTGGA ACTAATTGTC GCCATAACTC TCGAGAACAA GGATCGTCTA 
CCTCTTGTCT GGCCTCACGT CCGCCGCCAC CTCGAATGGC TTCTGTCACC ACGTTTCGGC 
CGGTGCCCGG TGCTCGTCGA ACGGGCCGTC GTCGGGCTTT TACGTGTAGC CAATCGGAAT 
CTATTCCGTG ATAACACAGT TTCCGACGAT GTTCTTCACT CGCTGTCGAT GCTCCTGCGA 
CTTTCCCCAA AGGCGCTCTT CATTTTCAGT CGGCAAATCG CTTTTGGGCT CTACGAGCTC 
ATTCGAGCAA ACGCGGCGAA TGTGCACAAA AAGGAGCACT GGGCAGTGCT CTTTGCGTTA 
CTTGAAGCCG CTGGAGCAGC AGTTCTACCT GATGATTATG TGATGATGAC GACGACGGAA 
AAGCAGCAGC AGAGCTTGAG AGTTGGAGGG GATCAGCAGC AGCAGAGAAT GGCTTATTCG 
GATGTTGAAG GAGCATCTGG AAGAGGTGGT GGAGCACATG AAGAAAGAGC ATATACATCG 
GAGGGAGAGG AGAGACGGCG AGGCGGTTAT GACTCCAACA GTGACCTGGA GAGCCGTGTG 
GACTCTGCTG GAAGCCTACT CGGAGCCCAG AAACAACCGG CCGACTGGAT CCATCTGGAC 
CATAAAGATG CGGCAAAAGC CACCGAAGAA GCGCTCACAG CTCTCGGAGC CAACGTTGTT 
AGCAGCAAAA AGAATTTCCG TCAATTTGGA AGCCTTGTGC TCCGGAATGG GCTCGGAAGA 
CATGAGCCAG CAGCTTTTTT GAAAGTCTGT GAGTGCCTGG CATTCTTGCT CCGCGACGCT 
GTTCACGTGA CACCGGACAA CTTTGAATCG AGCCTTCAAT GCCTTCGAAC AATGGTTGAA 
GCAAGTCTGG ATGGAGGTGT CTACGCAGCT GGGCCATTGA GTGGAGATGC TCAGAATCGG 
CTGAGGAGCA ATGTTACAGA TGAGAAAGCT GTTAAGAAAC ATCATCATCA TCATCATGGG 
CATAAGAAGA AGGAATTGTG TACGGATGTT ACTGAAGATG CTGACGAGAG CCGAAATGAA 
GAGCAACAGT TGATTGGGAA TTATCAACAG ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC 
CAACTCCATT CTCAAACTCC GGCAATCTTT GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT 
GCATCGGATC TGGCGACTGT CGCGTTCATA TGGACAGATA TCTGGCGCCC GTTGCTCCAA 
GCAATTGGAC GGCTCAGCTG TGATTGCCGA CGAGGTGTCC GTGCGGCGGC GTTGACTCAT 
TTGCAGCGCG CCTTCCTGCC GGCAAATATG GCAACTCTTG GAGCCGCAGA ATGGCAATCA 
TGCTTTGGAG AAGTGCTCTT CCCATTGCTC ACTAAGCTTC TGGAGCCATT CTCTCAAATG 
GATCCGATTG GAATGGAGGA TACACGTGTT CGGACACTTC AAATCGTCGC GAAAACTCTT 
TTAAATCATC TCTCAGCCCT GTCGGCTCTT GATTCATTCC CGGATCTTTG GATGCTTCTG 
CTCGACTATA TGGAGCAATA TCTTCGAGTT GATAGCTGTG GAAATTTGAA TGAAGCAGTG 
CCGGAATCGC TGAAAAATAT GCTTCTCGTT ATGGATAGTA CGGGAATTTT CGCTGCAACA 
CCTCGGCTTT ATGATGTGAC TGTGGAGAGG CTCAACAAAT TTATGCCCGA ATTGATTAAG 
GATACGATTC CGAATCCACC ACGGCCAGGG CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG 
AAGGAGCATG CTTCTGGGCT TGAGCCACCA CCACCATCAT CCAACTCAAC AGCCGCCACT 
TCAACATCTG ATCCTTCGAT CGCTACTGCA CAATCTTCAA TTTCGACAGC TTCATCTGTT 
GTTGGACCAC TTGTTACATG TCCAGAAGAT GCTGGAATTA GTGCTCCGAT TCCAATACAA 
CATCCATTGA CTGAAGTAAT TGTTCACTCG GGACCAACAT CACCAATTGG CTCACCACCA 
CAAACTGAGC CACCAGCTTC AAGTCCCCCG CAACACCAAC ATTCGGAGCA CCAACAATAT 
GAGCAATATC GACAGCAGCA GGCAGCGGCT GCACAGCAAT ATCAGCAGTA TAATCAGAAC 
TATCCCCAAC AACAGCAGCA ACAGCAGCAA CAATATGCAT ATTCACCAGA ACACGCGGCA 
TACTATCAGC AACAATATGC ACACCAACAA CAGCAATATG CAGAGCATTA TGCCAATCAA 
TATCAGCACT ATCAACAGCA GCAACAGCAA CAACAGCAGC ACCCAGTAAA TCCAACGAGT 
CCCAGTGTTC ACGGACAGTA TTCGGTGGCG AATCCTCTTC CACTGCCGGC TCATCCAGCT 
TATCATCCGA TTGTTGCACC ATCAGTGAAC AGTGCATTCA CCCATGTGTA CACTCCGCCA 
CAAAATAATG CTCCTGCTCT TGCTCCATCT GCTCCAACAA CAACGTCAGC AGATTCTCCG 
TATTTCACCC CGATTCCCTA CAATCCGAGC CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255140.1
CDS	21..5948
Translation	MATNGVYIVMGEANCVVALLNKARRQYQLSQVPTLEDTDPLLRNFTDLKEVLNEVADLADMNPQTYLSPFLDVIKAQNTNGPITEAALAAVAKFLNYGLIDASSIKAANAVESIAYAVVHTKFIGGKSTGSDECVLFKILQVLRSLLLSPPGILLSNEAVCDMMQSCFRIVFEQNLSLLLRKAAESTLADMTQLIFTRLPTFVEDTRHPYIRQLVNPTEKRQKRKKKRQLSVHIETKAKEPENVPTEMTKLIGEAAETAETDGAANLGYDVVLTTDPPVDTVTHPDPPIEEIIKLAEPISAGDEADSESEGGGGEEHHERPPVRAHAGLQREIVSDEEEIDTEQTVGGEEKMPYGLPCCRELLRFLITMTNPVDRHNTESMVILGLNLLIVALEAIADFLPNYDILMPLIKNELCRNLLQLLDTNRLPVLAATNRCCFLLFESMRMHMKFQLESYLKKLQSIVLTEEKQHENGGGGTEQKEMALESLVQLWRIPGLVTEMYLNFDCDLYCGNIFEDLTKLLVENSFPTVGGHTASLLSLDALLVVIETIEQNCEDRENGRGEVAKEQEHKDLKKLGLPVLSGYDLAKKMAISTGGKASPMPVSSSIVLRSNRHAPSTELPSMSQIIEQKKRKRLIAEGTELFNQSPKKGIAFLREKGILGHDEQSLVQWLRTNPQLDKKAIADYICNRKHAEVLNAFVKSFPFENTRLDVALRMFLETFRLPGESAEIALVMQHFSEEWFRANNEPFFHVDAAFTLSYAIIMLNVDQHNPQAKRSQPPMTVDCFRRNLSGTNDSRDFDPEMLADMYQAIKTEEIVMPAEQKGTVKEDYMWKVLLRRGETAEGSFYHAPTGWNDHDLFAVCWGPAVAALSYVFDKSEHEQILQKALTGYRKCAKIAAYYGMKEVFDNLCIHLCKFTTLTSMRDGGAGGGADEDVDLSAAALLSHSSSPEAVALAFGENHKAQLATRTLFYLVHENGNILREGWRNLFEALLQLFRARLLPAELTEVEDYVDEKGWVNIQRVHQKELPHTRNDSGLLSWFGLGGGASEADRRKPTQEQLSSMKLASQVISECRPSQIVADSKYLTSTSLAELLSSIAANSAQIVEQAEPQQKTASLSGEDEDALVFYLELIVAITLENKDRLPLVWPHVRRHLEWLLSPRFGRCPVLVERAVVGLLRVANRNLFRDNTVSDDVLHSLSMLLRLSPKALFIFSRQIAFGLYELIRANAANVHKKEHWAVLFALLEAAGAAVLPDDYVMMTTTEKQQQSLRVGGDQQQQRMAYSDVEGASGRGGGAHEERAYTSEGEERRRGGYDSNSDLESRVDSAGSLLGAQKQPADWIHLDHKDAAKATEEALTALGANVVSSKKNFRQFGSLVLRNGLGRHEPAAFLKVCECLAFLLRDAVHVTPDNFESSLQCLRTMVEASLDGGVYAAGPLSGDAQNRLRSNVTDEKAVKKHHHHHHGHKKKELCTDVTEDADESRNEEQQLIGNYQQMSLHLLDLCSQLHSQTPAIFAKWAQGASPAASDLATVAFIWTDIWRPLLQAIGRLSCDCRRGVRAAALTHLQRAFLPANMATLGAAEWQSCFGEVLFPLLTKLLEPFSQMDPIGMEDTRVRTLQIVAKTLLNHLSALSALDSFPDLWMLLLDYMEQYLRVDSCGNLNEAVPESLKNMLLVMDSTGIFAATPRLYDVTVERLNKFMPELIKDTIPNPPRPGQQQSEASEPKKEHASGLEPPPPSSNSTAATSTSDPSIATAQSSISTASSVVGPLVTCPEDAGISAPIPIQHPLTEVIVHSGPTSPIGSPPQTEPPASSPPQHQHSEHQQYEQYRQQQAAAAQQYQQYNQNYPQQQQQQQQQYAYSPEHAAYYQQQYAHQQQQYAEHYANQYQHYQQQQQQQQQHPVNPTSPSVHGQYSVANPLPLPAHPAYHPIVAPSVNSAFTHVYTPPQNNAPALAPSAPTTTSADSPYFTPIPYNPSQQEKP

Target ORF information:

RefSeq Version	NM_001268211.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (gbf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268211.2

ORF Insert Sequence:

ATGGCGACCA ACGGCGTCTA CATCGTGATG GGCGAGGCGA ATTGTGTCGT CGCGCTGCTC 
AATAAGGCTC GGAGACAATA CCAACTGTCA CAAGTGCCGA CGCTCGAAGA CACGGATCCA 
CTGCTGCGAA ACTTCACAGA TCTCAAGGAA GTGCTGAATG AGGTTGCCGA TCTGGCAGAT 
ATGAATCCAC AAACGTATCT ATCACCGTTT CTCGACGTAA TAAAGGCTCA GAACACGAAT 
GGACCGATTA CGGAAGCCGC GCTGGCCGCT GTCGCCAAAT TTCTGAACTA CGGGCTCATT 
GACGCGAGCA GTATTAAGGC GGCGAACGCT GTCGAGTCTA TCGCCTACGC TGTGGTTCAT 
ACAAAGTTTA TCGGCGGGAA AAGCACTGGA AGTGATGAAT GTGTCCTTTT TAAGATTCTA 
CAGGTGCTCC GCTCGCTGCT TCTCTCCCCT CCAGGCATCC TTTTGTCTAA TGAAGCCGTC 
TGTGATATGA TGCAGTCTTG TTTCAGAATT GTGTTCGAGC AGAACCTCTC TCTGCTTCTT 
AGAAAGGCCG CCGAATCGAC GCTAGCCGAC ATGACACAGC TCATTTTCAC CAGATTGCCA 
ACTTTTGTCG AAGACACCCG TCATCCATAC ATTCGGCAGC TGGTAAATCC CACTGAAAAA 
CGACAAAAAC GGAAGAAAAA GCGTCAATTG TCAGTGCATA TTGAGACGAA GGCCAAGGAA 
CCGGAGAATG TGCCAACGGA AATGACAAAA TTGATTGGAG AAGCTGCAGA AACTGCTGAA 
ACTGACGGAG CAGCGAATCT TGGCTACGAC GTAGTGCTCA CGACGGATCC ACCAGTCGAT 
ACAGTAACCC ACCCGGATCC ACCAATTGAA GAAATCATAA AGCTTGCTGA GCCAATTTCA 
GCCGGAGATG AAGCCGATTC AGAATCAGAA GGCGGTGGCG GTGAAGAGCA TCACGAACGT 
CCCCCCGTTC GGGCTCACGC TGGATTACAG AGAGAAATTG TGTCGGATGA AGAGGAAATC 
GATACGGAGC AAACTGTTGG AGGAGAAGAA AAAATGCCCT ATGGGCTCCC ATGTTGTAGA 
GAGCTTTTGA GATTCCTCAT CACAATGACC AACCCAGTTG ACCGTCACAA TACTGAATCA 
ATGGTAATTC TCGGCTTAAA CTTGCTCATT GTTGCTCTGG AAGCCATCGC CGACTTCCTT 
CCAAACTACG ATATTCTCAT GCCACTTATC AAAAATGAGC TCTGTCGAAA TTTGCTCCAA 
CTGCTCGACA CGAATCGCCT ACCAGTGCTC GCCGCCACAA ATCGCTGCTG TTTTTTGCTG 
TTTGAATCGA TGAGAATGCA CATGAAATTC CAGTTGGAAA GCTATTTGAA GAAGCTTCAA 
TCGATTGTGC TCACCGAGGA AAAGCAGCAT GAAAACGGTG GTGGTGGAAC GGAGCAAAAA 
GAAATGGCTT TGGAGTCACT TGTTCAGTTG TGGAGAATTC CCGGATTGGT TACCGAGATG 
TATCTCAATT TTGATTGTGA TCTCTATTGC GGAAATATCT TCGAAGATTT GACAAAGCTT 
CTCGTTGAGA ACTCATTTCC AACAGTTGGT GGACACACAG CCTCGCTGCT CTCTCTGGAT 
GCTCTTTTAG TCGTTATTGA GACTATTGAA CAGAATTGTG AAGATCGAGA GAATGGACGA 
GGAGAAGTTG CCAAAGAGCA AGAGCACAAG GACTTAAAGA AGCTCGGGCT CCCAGTGCTC 
AGCGGCTATG ATCTAGCGAA GAAAATGGCG ATTTCAACGG GAGGAAAAGC TTCTCCAATG 
CCTGTGAGCT CATCAATTGT GCTCCGTTCG AATCGACATG CTCCGTCAAC AGAACTCCCA 
TCGATGAGCC AAATAATTGA ACAAAAGAAG CGAAAACGAC TGATTGCCGA AGGAACCGAA 
CTGTTCAATC AAAGTCCCAA AAAAGGAATC GCTTTTCTCC GGGAAAAGGG AATTCTGGGA 
CACGATGAGC AGAGCCTTGT GCAGTGGCTC CGAACGAATC CACAGTTGGA TAAGAAGGCT 
ATTGCAGATT ATATTTGTAA TCGGAAGCAT GCTGAGGTTC TCAACGCGTT TGTCAAGAGT 
TTTCCATTCG AGAATACCCG CCTGGACGTT GCTCTCCGAA TGTTCCTCGA AACGTTCCGC 
CTGCCTGGAG AATCGGCTGA AATTGCACTT GTAATGCAAC ATTTCTCCGA AGAATGGTTC 
CGAGCCAACA ATGAGCCATT TTTCCATGTC GATGCCGCTT TTACTCTCTC TTATGCGATT 
ATTATGCTCA ATGTTGATCA GCATAATCCG CAGGCCAAAC GAAGTCAACC ACCAATGACA 
GTCGACTGTT TCCGTCGGAA TCTCTCTGGA ACCAACGATT CCCGAGATTT CGACCCAGAA 
ATGCTCGCCG ACATGTACCA GGCCATCAAA ACCGAGGAAA TCGTGATGCC AGCCGAGCAA 
AAGGGCACCG TAAAGGAGGA TTACATGTGG AAAGTGTTGC TCCGTCGTGG TGAGACTGCC 
GAAGGATCGT TTTATCATGC TCCAACCGGA TGGAATGATC ATGATCTATT CGCAGTTTGC 
TGGGGTCCTG CTGTCGCTGC TCTCAGCTAT GTATTCGATA AATCGGAGCA CGAGCAGATA 
CTTCAGAAGG CTCTGACAGG CTATCGGAAG TGTGCGAAAA TCGCTGCCTA CTACGGGATG 
AAGGAGGTTT TCGATAATCT ATGTATTCAT CTTTGCAAGT TCACAACGTT AACTTCAATG 
CGGGATGGTG GAGCTGGTGG TGGAGCCGAT GAGGATGTGG ATCTTTCGGC GGCTGCTCTT 
TTGAGCCATA GCTCCTCACC TGAAGCTGTC GCCCTCGCAT TTGGAGAGAA TCATAAGGCT 
CAACTTGCCA CTCGAACCCT GTTCTATTTG GTTCATGAGA ATGGGAATAT ACTCAGAGAG 
GGATGGCGCA ACTTGTTTGA GGCTCTTCTG CAGCTGTTTA GAGCACGATT GCTTCCTGCA 
GAGCTCACTG AAGTCGAGGA TTATGTTGAT GAGAAGGGAT GGGTTAATAT TCAGAGGGTT 
CATCAGAAAG AGCTCCCACA CACCCGCAAC GATTCAGGCC TTCTTTCATG GTTCGGACTC 
GGTGGAGGCG CTTCAGAAGC GGATCGCCGT AAGCCGACCC AAGAGCAACT GAGCTCGATG 
AAGCTCGCGT CACAAGTCAT TTCGGAGTGC CGTCCGTCGC AAATTGTGGC TGATAGCAAG 
TATCTGACGA GCACATCGCT CGCCGAGCTC CTCTCATCGA TTGCGGCGAA CAGTGCGCAA 
ATTGTTGAGC AAGCCGAGCC ACAGCAAAAG ACCGCAAGCC TGAGTGGAGA GGATGAGGAT 
GCGCTGGTCT TCTACCTGGA ACTAATTGTC GCCATAACTC TCGAGAACAA GGATCGTCTA 
CCTCTTGTCT GGCCTCACGT CCGCCGCCAC CTCGAATGGC TTCTGTCACC ACGTTTCGGC 
CGGTGCCCGG TGCTCGTCGA ACGGGCCGTC GTCGGGCTTT TACGTGTAGC CAATCGGAAT 
CTATTCCGTG ATAACACAGT TTCCGACGAT GTTCTTCACT CGCTGTCGAT GCTCCTGCGA 
CTTTCCCCAA AGGCGCTCTT CATTTTCAGT CGGCAAATCG CTTTTGGGCT CTACGAGCTC 
ATTCGAGCAA ACGCGGCGAA TGTGCACAAA AAGGAGCACT GGGCAGTGCT CTTTGCGTTA 
CTTGAAGCCG CTGGAGCAGC AGTTCTACCT GATGATTATG TGATGATGAC GACGACGGAA 
AAGCAGCAGC AGAGCTTGAG AGTTGGAGGG GATCAGCAGC AGCAGAGAAT GGCTTATTCG 
GATGTTGAAG GAGCATCTGG AAGAGGTGGT GGAGCACATG AAGAAAGAGC ATATACATCG 
GAGGGAGAGG AGAGACGGCG AGGCGGTTAT GACTCCAACA GTGACCTGGA GAGCCGTGTG 
GACTCTGCTG GAAGCCTACT CGGAGCCCAG AAACAACCGG CCGACTGGAT CCATCTGGAC 
CATAAAGATG CGGCAAAAGC CACCGAAGAA GCGCTCACAG CTCTCGGAGC CAACGTTGTT 
AGCAGCAAAA AGAATTTCCG TCAATTTGGA AGCCTTGTGC TCCGGAATGG GCTCGGAAGA 
CATGAGCCAG CAGCTTTTTT GAAAGTCTGT GAGTGCCTGG CATTCTTGCT CCGCGACGCT 
GTTCACGTGA CACCGGACAA CTTTGAATCG AGCCTTCAAT GCCTTCGAAC AATGGTTGAA 
GCAAGTCTGG ATGGAGGTGT CTACGCAGCT GGGCCATTGA GTGGAGATGC TCAGAATCGG 
CTGAGGAGCA ATGTTACAGA TGAGAAAGCT GTTAAGAAAC ATCATCATCA TCATCATGGG 
CATAAGAAGA AGGAATTGTG TACGGATGTT ACTGAAGATG CTGACGAGAG CCGAAATGAA 
GAGCAACAGT TGATTGGGAA TTATCAACAG ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC 
CAACTCCATT CTCAAACTCC GGCAATCTTT GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT 
GCATCGGATC TGGCGACTGT CGCGTTCATA TGGACAGATA TCTGGCGCCC GTTGCTCCAA 
GCAATTGGAC GGCTCAGCTG TGATTGCCGA CGAGGTGTCC GTGCGGCGGC GTTGACTCAT 
TTGCAGCGCG CCTTCCTGCC GGCAAATATG GCAACTCTTG GAGCCGCAGA ATGGCAATCA 
TGCTTTGGAG AAGTGCTCTT CCCATTGCTC ACTAAGCTTC TGGAGCCATT CTCTCAAATG 
GATCCGATTG GAATGGAGGA TACACGTGTT CGGACACTTC AAATCGTCGC GAAAACTCTT 
TTAAATCATC TCTCAGCCCT GTCGGCTCTT GATTCATTCC CGGATCTTTG GATGCTTCTG 
CTCGACTATA TGGAGCAATA TCTTCGAGTT GATAGCTGTG GAAATTTGAA TGAAGCAGTG 
CCGGAATCGC TGAAAAATAT GCTTCTCGTT ATGGATAGTA CGGGAATTTT CGCTGCAACA 
CCTCGGCTTT ATGATGTGAC TGTGGAGAGG CTCAACAAAT TTATGCCCGA ATTGATTAAG 
GATACGATTC CGAATCCACC ACGGCCAGGG CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG 
AAGGAGCATG CTTCTGGGCT TGAGCCACCA CCACCATCAT CCAACTCAAC AGCCGCCACT 
TCAACATCTG ATCCTTCGAT CGCTACTGCA CAATCTTCAA TTTCGACAGC TTCATCTGTT 
GTTGGACCAC TTGTTACATG TCCAGAAGAT GCTGGAATTA GTGCTCCGAT TCCAATACAA 
CATCCATTGA CTGAAGTAAT TGTTCACTCG GGACCAACAT CACCAATTGG CTCACCACCA 
CAAACTGAGC CACCAGCTTC AAGTCCCCCG CAACACCAAC ATTCGGAGCA CCAACAATAT 
GAGCAATATC GACAGCAGCA GGCAGCGGCT GCACAGCAAT ATCAGCAGTA TAATCAGAAC 
TATCCCCAAC AACAGCAGCA ACAGCAGCAA CAATATGCAT ATTCACCAGA ACACGCGGCA 
TACTATCAGC AACAATATGC ACACCAACAA CAGCAATATG CAGAGCATTA TGCCAATCAA 
TATCAGCACT ATCAACAGCA GCAACAGCAA CAACAGCAGC ACCCAGTAAA TCCAACGAGT 
CCCAGTGTTC ACGGACAGTA TTCGGTGGCG AATCCTCTTC CACTGCCGGC TCATCCAGCT 
TATCATCCGA TTGTTGCACC ATCAGTGAAC AGTGCATTCA CCCATGTGTA CACTCCGCCA 
CAAAATAATG CTCCTGCTCT TGCTCCATCT GCTCCAACAA CAACGTCAGC AGATTCTCCG 
TATTTCACCC CGATTCCCTA CAATCCGAGC CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbf-1 ( NM_001268211.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255140.1 Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.

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CloneID	OCa352773
Clone ID Related Accession (Same CDS sequence)	NM_001268211.1 , NM_001268211.2
Accession Version	NM_001268211.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGCGACCA ACGGCGTCTA CATCGTGATG GGCGAGGCGA ATTGTGTCGT CGCGCTGCTC 
AATAAGGCTC GGAGACAATA CCAACTGTCA CAAGTGCCGA CGCTCGAAGA CACGGATCCA 
CTGCTGCGAA ACTTCACAGA TCTCAAGGAA GTGCTGAATG AGGTTGCCGA TCTGGCAGAT 
ATGAATCCAC AAACGTATCT ATCACCGTTT CTCGACGTAA TAAAGGCTCA GAACACGAAT 
GGACCGATTA CGGAAGCCGC GCTGGCCGCT GTCGCCAAAT TTCTGAACTA CGGGCTCATT 
GACGCGAGCA GTATTAAGGC GGCGAACGCT GTCGAGTCTA TCGCCTACGC TGTGGTTCAT 
ACAAAGTTTA TCGGCGGGAA AAGCACTGGA AGTGATGAAT GTGTCCTTTT TAAGATTCTA 
CAGGTGCTCC GCTCGCTGCT TCTCTCCCCT CCAGGCATCC TTTTGTCTAA TGAAGCCGTC 
TGTGATATGA TGCAGTCTTG TTTCAGAATT GTGTTCGAGC AGAACCTCTC TCTGCTTCTT 
AGAAAGGCCG CCGAATCGAC GCTAGCCGAC ATGACACAGC TCATTTTCAC CAGATTGCCA 
ACTTTTGTCG AAGACACCCG TCATCCATAC ATTCGGCAGC TGGTAAATCC CACTGAAAAA 
CGACAAAAAC GGAAGAAAAA GCGTCAATTG TCAGTGCATA TTGAGACGAA GGCCAAGGAA 
CCGGAGAATG TGCCAACGGA AATGACAAAA TTGATTGGAG AAGCTGCAGA AACTGCTGAA 
ACTGACGGAG CAGCGAATCT TGGCTACGAC GTAGTGCTCA CGACGGATCC ACCAGTCGAT 
ACAGTAACCC ACCCGGATCC ACCAATTGAA GAAATCATAA AGCTTGCTGA GCCAATTTCA 
GCCGGAGATG AAGCCGATTC AGAATCAGAA GGCGGTGGCG GTGAAGAGCA TCACGAACGT 
CCCCCCGTTC GGGCTCACGC TGGATTACAG AGAGAAATTG TGTCGGATGA AGAGGAAATC 
GATACGGAGC AAACTGTTGG AGGAGAAGAA AAAATGCCCT ATGGGCTCCC ATGTTGTAGA 
GAGCTTTTGA GATTCCTCAT CACAATGACC AACCCAGTTG ACCGTCACAA TACTGAATCA 
ATGGTAATTC TCGGCTTAAA CTTGCTCATT GTTGCTCTGG AAGCCATCGC CGACTTCCTT 
CCAAACTACG ATATTCTCAT GCCACTTATC AAAAATGAGC TCTGTCGAAA TTTGCTCCAA 
CTGCTCGACA CGAATCGCCT ACCAGTGCTC GCCGCCACAA ATCGCTGCTG TTTTTTGCTG 
TTTGAATCGA TGAGAATGCA CATGAAATTC CAGTTGGAAA GCTATTTGAA GAAGCTTCAA 
TCGATTGTGC TCACCGAGGA AAAGCAGCAT GAAAACGGTG GTGGTGGAAC GGAGCAAAAA 
GAAATGGCTT TGGAGTCACT TGTTCAGTTG TGGAGAATTC CCGGATTGGT TACCGAGATG 
TATCTCAATT TTGATTGTGA TCTCTATTGC GGAAATATCT TCGAAGATTT GACAAAGCTT 
CTCGTTGAGA ACTCATTTCC AACAGTTGGT GGACACACAG CCTCGCTGCT CTCTCTGGAT 
GCTCTTTTAG TCGTTATTGA GACTATTGAA CAGAATTGTG AAGATCGAGA GAATGGACGA 
GGAGAAGTTG CCAAAGAGCA AGAGCACAAG GACTTAAAGA AGCTCGGGCT CCCAGTGCTC 
AGCGGCTATG ATCTAGCGAA GAAAATGGCG ATTTCAACGG GAGGAAAAGC TTCTCCAATG 
CCTGTGAGCT CATCAATTGT GCTCCGTTCG AATCGACATG CTCCGTCAAC AGAACTCCCA 
TCGATGAGCC AAATAATTGA ACAAAAGAAG CGAAAACGAC TGATTGCCGA AGGAACCGAA 
CTGTTCAATC AAAGTCCCAA AAAAGGAATC GCTTTTCTCC GGGAAAAGGG AATTCTGGGA 
CACGATGAGC AGAGCCTTGT GCAGTGGCTC CGAACGAATC CACAGTTGGA TAAGAAGGCT 
ATTGCAGATT ATATTTGTAA TCGGAAGCAT GCTGAGGTTC TCAACGCGTT TGTCAAGAGT 
TTTCCATTCG AGAATACCCG CCTGGACGTT GCTCTCCGAA TGTTCCTCGA AACGTTCCGC 
CTGCCTGGAG AATCGGCTGA AATTGCACTT GTAATGCAAC ATTTCTCCGA AGAATGGTTC 
CGAGCCAACA ATGAGCCATT TTTCCATGTC GATGCCGCTT TTACTCTCTC TTATGCGATT 
ATTATGCTCA ATGTTGATCA GCATAATCCG CAGGCCAAAC GAAGTCAACC ACCAATGACA 
GTCGACTGTT TCCGTCGGAA TCTCTCTGGA ACCAACGATT CCCGAGATTT CGACCCAGAA 
ATGCTCGCCG ACATGTACCA GGCCATCAAA ACCGAGGAAA TCGTGATGCC AGCCGAGCAA 
AAGGGCACCG TAAAGGAGGA TTACATGTGG AAAGTGTTGC TCCGTCGTGG TGAGACTGCC 
GAAGGATCGT TTTATCATGC TCCAACCGGA TGGAATGATC ATGATCTATT CGCAGTTTGC 
TGGGGTCCTG CTGTCGCTGC TCTCAGCTAT GTATTCGATA AATCGGAGCA CGAGCAGATA 
CTTCAGAAGG CTCTGACAGG CTATCGGAAG TGTGCGAAAA TCGCTGCCTA CTACGGGATG 
AAGGAGGTTT TCGATAATCT ATGTATTCAT CTTTGCAAGT TCACAACGTT AACTTCAATG 
CGGGATGGTG GAGCTGGTGG TGGAGCCGAT GAGGATGTGG ATCTTTCGGC GGCTGCTCTT 
TTGAGCCATA GCTCCTCACC TGAAGCTGTC GCCCTCGCAT TTGGAGAGAA TCATAAGGCT 
CAACTTGCCA CTCGAACCCT GTTCTATTTG GTTCATGAGA ATGGGAATAT ACTCAGAGAG 
GGATGGCGCA ACTTGTTTGA GGCTCTTCTG CAGCTGTTTA GAGCACGATT GCTTCCTGCA 
GAGCTCACTG AAGTCGAGGA TTATGTTGAT GAGAAGGGAT GGGTTAATAT TCAGAGGGTT 
CATCAGAAAG AGCTCCCACA CACCCGCAAC GATTCAGGCC TTCTTTCATG GTTCGGACTC 
GGTGGAGGCG CTTCAGAAGC GGATCGCCGT AAGCCGACCC AAGAGCAACT GAGCTCGATG 
AAGCTCGCGT CACAAGTCAT TTCGGAGTGC CGTCCGTCGC AAATTGTGGC TGATAGCAAG 
TATCTGACGA GCACATCGCT CGCCGAGCTC CTCTCATCGA TTGCGGCGAA CAGTGCGCAA 
ATTGTTGAGC AAGCCGAGCC ACAGCAAAAG ACCGCAAGCC TGAGTGGAGA GGATGAGGAT 
GCGCTGGTCT TCTACCTGGA ACTAATTGTC GCCATAACTC TCGAGAACAA GGATCGTCTA 
CCTCTTGTCT GGCCTCACGT CCGCCGCCAC CTCGAATGGC TTCTGTCACC ACGTTTCGGC 
CGGTGCCCGG TGCTCGTCGA ACGGGCCGTC GTCGGGCTTT TACGTGTAGC CAATCGGAAT 
CTATTCCGTG ATAACACAGT TTCCGACGAT GTTCTTCACT CGCTGTCGAT GCTCCTGCGA 
CTTTCCCCAA AGGCGCTCTT CATTTTCAGT CGGCAAATCG CTTTTGGGCT CTACGAGCTC 
ATTCGAGCAA ACGCGGCGAA TGTGCACAAA AAGGAGCACT GGGCAGTGCT CTTTGCGTTA 
CTTGAAGCCG CTGGAGCAGC AGTTCTACCT GATGATTATG TGATGATGAC GACGACGGAA 
AAGCAGCAGC AGAGCTTGAG AGTTGGAGGG GATCAGCAGC AGCAGAGAAT GGCTTATTCG 
GATGTTGAAG GAGCATCTGG AAGAGGTGGT GGAGCACATG AAGAAAGAGC ATATACATCG 
GAGGGAGAGG AGAGACGGCG AGGCGGTTAT GACTCCAACA GTGACCTGGA GAGCCGTGTG 
GACTCTGCTG GAAGCCTACT CGGAGCCCAG AAACAACCGG CCGACTGGAT CCATCTGGAC 
CATAAAGATG CGGCAAAAGC CACCGAAGAA GCGCTCACAG CTCTCGGAGC CAACGTTGTT 
AGCAGCAAAA AGAATTTCCG TCAATTTGGA AGCCTTGTGC TCCGGAATGG GCTCGGAAGA 
CATGAGCCAG CAGCTTTTTT GAAAGTCTGT GAGTGCCTGG CATTCTTGCT CCGCGACGCT 
GTTCACGTGA CACCGGACAA CTTTGAATCG AGCCTTCAAT GCCTTCGAAC AATGGTTGAA 
GCAAGTCTGG ATGGAGGTGT CTACGCAGCT GGGCCATTGA GTGGAGATGC TCAGAATCGG 
CTGAGGAGCA ATGTTACAGA TGAGAAAGCT GTTAAGAAAC ATCATCATCA TCATCATGGG 
CATAAGAAGA AGGAATTGTG TACGGATGTT ACTGAAGATG CTGACGAGAG CCGAAATGAA 
GAGCAACAGT TGATTGGGAA TTATCAACAG ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC 
CAACTCCATT CTCAAACTCC GGCAATCTTT GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT 
GCATCGGATC TGGCGACTGT CGCGTTCATA TGGACAGATA TCTGGCGCCC GTTGCTCCAA 
GCAATTGGAC GGCTCAGCTG TGATTGCCGA CGAGGTGTCC GTGCGGCGGC GTTGACTCAT 
TTGCAGCGCG CCTTCCTGCC GGCAAATATG GCAACTCTTG GAGCCGCAGA ATGGCAATCA 
TGCTTTGGAG AAGTGCTCTT CCCATTGCTC ACTAAGCTTC TGGAGCCATT CTCTCAAATG 
GATCCGATTG GAATGGAGGA TACACGTGTT CGGACACTTC AAATCGTCGC GAAAACTCTT 
TTAAATCATC TCTCAGCCCT GTCGGCTCTT GATTCATTCC CGGATCTTTG GATGCTTCTG 
CTCGACTATA TGGAGCAATA TCTTCGAGTT GATAGCTGTG GAAATTTGAA TGAAGCAGTG 
CCGGAATCGC TGAAAAATAT GCTTCTCGTT ATGGATAGTA CGGGAATTTT CGCTGCAACA 
CCTCGGCTTT ATGATGTGAC TGTGGAGAGG CTCAACAAAT TTATGCCCGA ATTGATTAAG 
GATACGATTC CGAATCCACC ACGGCCAGGG CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG 
AAGGAGCATG CTTCTGGGCT TGAGCCACCA CCACCATCAT CCAACTCAAC AGCCGCCACT 
TCAACATCTG ATCCTTCGAT CGCTACTGCA CAATCTTCAA TTTCGACAGC TTCATCTGTT 
GTTGGACCAC TTGTTACATG TCCAGAAGAT GCTGGAATTA GTGCTCCGAT TCCAATACAA 
CATCCATTGA CTGAAGTAAT TGTTCACTCG GGACCAACAT CACCAATTGG CTCACCACCA 
CAAACTGAGC CACCAGCTTC AAGTCCCCCG CAACACCAAC ATTCGGAGCA CCAACAATAT 
GAGCAATATC GACAGCAGCA GGCAGCGGCT GCACAGCAAT ATCAGCAGTA TAATCAGAAC 
TATCCCCAAC AACAGCAGCA ACAGCAGCAA CAATATGCAT ATTCACCAGA ACACGCGGCA 
TACTATCAGC AACAATATGC ACACCAACAA CAGCAATATG CAGAGCATTA TGCCAATCAA 
TATCAGCACT ATCAACAGCA GCAACAGCAA CAACAGCAGC ACCCAGTAAA TCCAACGAGT 
CCCAGTGTTC ACGGACAGTA TTCGGTGGCG AATCCTCTTC CACTGCCGGC TCATCCAGCT 
TATCATCCGA TTGTTGCACC ATCAGTGAAC AGTGCATTCA CCCATGTGTA CACTCCGCCA 
CAAAATAATG CTCCTGCTCT TGCTCCATCT GCTCCAACAA CAACGTCAGC AGATTCTCCG 
TATTTCACCC CGATTCCCTA CAATCCGAGC CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255140.1
CDS	1..5928
Translation	MATNGVYIVMGEANCVVALLNKARRQYQLSQVPTLEDTDPLLRNFTDLKEVLNEVADLADMNPQTYLSPFLDVIKAQNTNGPITEAALAAVAKFLNYGLIDASSIKAANAVESIAYAVVHTKFIGGKSTGSDECVLFKILQVLRSLLLSPPGILLSNEAVCDMMQSCFRIVFEQNLSLLLRKAAESTLADMTQLIFTRLPTFVEDTRHPYIRQLVNPTEKRQKRKKKRQLSVHIETKAKEPENVPTEMTKLIGEAAETAETDGAANLGYDVVLTTDPPVDTVTHPDPPIEEIIKLAEPISAGDEADSESEGGGGEEHHERPPVRAHAGLQREIVSDEEEIDTEQTVGGEEKMPYGLPCCRELLRFLITMTNPVDRHNTESMVILGLNLLIVALEAIADFLPNYDILMPLIKNELCRNLLQLLDTNRLPVLAATNRCCFLLFESMRMHMKFQLESYLKKLQSIVLTEEKQHENGGGGTEQKEMALESLVQLWRIPGLVTEMYLNFDCDLYCGNIFEDLTKLLVENSFPTVGGHTASLLSLDALLVVIETIEQNCEDRENGRGEVAKEQEHKDLKKLGLPVLSGYDLAKKMAISTGGKASPMPVSSSIVLRSNRHAPSTELPSMSQIIEQKKRKRLIAEGTELFNQSPKKGIAFLREKGILGHDEQSLVQWLRTNPQLDKKAIADYICNRKHAEVLNAFVKSFPFENTRLDVALRMFLETFRLPGESAEIALVMQHFSEEWFRANNEPFFHVDAAFTLSYAIIMLNVDQHNPQAKRSQPPMTVDCFRRNLSGTNDSRDFDPEMLADMYQAIKTEEIVMPAEQKGTVKEDYMWKVLLRRGETAEGSFYHAPTGWNDHDLFAVCWGPAVAALSYVFDKSEHEQILQKALTGYRKCAKIAAYYGMKEVFDNLCIHLCKFTTLTSMRDGGAGGGADEDVDLSAAALLSHSSSPEAVALAFGENHKAQLATRTLFYLVHENGNILREGWRNLFEALLQLFRARLLPAELTEVEDYVDEKGWVNIQRVHQKELPHTRNDSGLLSWFGLGGGASEADRRKPTQEQLSSMKLASQVISECRPSQIVADSKYLTSTSLAELLSSIAANSAQIVEQAEPQQKTASLSGEDEDALVFYLELIVAITLENKDRLPLVWPHVRRHLEWLLSPRFGRCPVLVERAVVGLLRVANRNLFRDNTVSDDVLHSLSMLLRLSPKALFIFSRQIAFGLYELIRANAANVHKKEHWAVLFALLEAAGAAVLPDDYVMMTTTEKQQQSLRVGGDQQQQRMAYSDVEGASGRGGGAHEERAYTSEGEERRRGGYDSNSDLESRVDSAGSLLGAQKQPADWIHLDHKDAAKATEEALTALGANVVSSKKNFRQFGSLVLRNGLGRHEPAAFLKVCECLAFLLRDAVHVTPDNFESSLQCLRTMVEASLDGGVYAAGPLSGDAQNRLRSNVTDEKAVKKHHHHHHGHKKKELCTDVTEDADESRNEEQQLIGNYQQMSLHLLDLCSQLHSQTPAIFAKWAQGASPAASDLATVAFIWTDIWRPLLQAIGRLSCDCRRGVRAAALTHLQRAFLPANMATLGAAEWQSCFGEVLFPLLTKLLEPFSQMDPIGMEDTRVRTLQIVAKTLLNHLSALSALDSFPDLWMLLLDYMEQYLRVDSCGNLNEAVPESLKNMLLVMDSTGIFAATPRLYDVTVERLNKFMPELIKDTIPNPPRPGQQQSEASEPKKEHASGLEPPPPSSNSTAATSTSDPSIATAQSSISTASSVVGPLVTCPEDAGISAPIPIQHPLTEVIVHSGPTSPIGSPPQTEPPASSPPQHQHSEHQQYEQYRQQQAAAAQQYQQYNQNYPQQQQQQQQQYAYSPEHAAYYQQQYAHQQQQYAEHYANQYQHYQQQQQQQQQHPVNPTSPSVHGQYSVANPLPLPAHPAYHPIVAPSVNSAFTHVYTPPQNNAPALAPSAPTTTSADSPYFTPIPYNPSQQEKP

Target ORF information:

RefSeq Version	NM_001268211.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268211.1

ORF Insert Sequence:

ATGGCGACCA ACGGCGTCTA CATCGTGATG GGCGAGGCGA ATTGTGTCGT CGCGCTGCTC 
AATAAGGCTC GGAGACAATA CCAACTGTCA CAAGTGCCGA CGCTCGAAGA CACGGATCCA 
CTGCTGCGAA ACTTCACAGA TCTCAAGGAA GTGCTGAATG AGGTTGCCGA TCTGGCAGAT 
ATGAATCCAC AAACGTATCT ATCACCGTTT CTCGACGTAA TAAAGGCTCA GAACACGAAT 
GGACCGATTA CGGAAGCCGC GCTGGCCGCT GTCGCCAAAT TTCTGAACTA CGGGCTCATT 
GACGCGAGCA GTATTAAGGC GGCGAACGCT GTCGAGTCTA TCGCCTACGC TGTGGTTCAT 
ACAAAGTTTA TCGGCGGGAA AAGCACTGGA AGTGATGAAT GTGTCCTTTT TAAGATTCTA 
CAGGTGCTCC GCTCGCTGCT TCTCTCCCCT CCAGGCATCC TTTTGTCTAA TGAAGCCGTC 
TGTGATATGA TGCAGTCTTG TTTCAGAATT GTGTTCGAGC AGAACCTCTC TCTGCTTCTT 
AGAAAGGCCG CCGAATCGAC GCTAGCCGAC ATGACACAGC TCATTTTCAC CAGATTGCCA 
ACTTTTGTCG AAGACACCCG TCATCCATAC ATTCGGCAGC TGGTAAATCC CACTGAAAAA 
CGACAAAAAC GGAAGAAAAA GCGTCAATTG TCAGTGCATA TTGAGACGAA GGCCAAGGAA 
CCGGAGAATG TGCCAACGGA AATGACAAAA TTGATTGGAG AAGCTGCAGA AACTGCTGAA 
ACTGACGGAG CAGCGAATCT TGGCTACGAC GTAGTGCTCA CGACGGATCC ACCAGTCGAT 
ACAGTAACCC ACCCGGATCC ACCAATTGAA GAAATCATAA AGCTTGCTGA GCCAATTTCA 
GCCGGAGATG AAGCCGATTC AGAATCAGAA GGCGGTGGCG GTGAAGAGCA TCACGAACGT 
CCCCCCGTTC GGGCTCACGC TGGATTACAG AGAGAAATTG TGTCGGATGA AGAGGAAATC 
GATACGGAGC AAACTGTTGG AGGAGAAGAA AAAATGCCCT ATGGGCTCCC ATGTTGTAGA 
GAGCTTTTGA GATTCCTCAT CACAATGACC AACCCAGTTG ACCGTCACAA TACTGAATCA 
ATGGTAATTC TCGGCTTAAA CTTGCTCATT GTTGCTCTGG AAGCCATCGC CGACTTCCTT 
CCAAACTACG ATATTCTCAT GCCACTTATC AAAAATGAGC TCTGTCGAAA TTTGCTCCAA 
CTGCTCGACA CGAATCGCCT ACCAGTGCTC GCCGCCACAA ATCGCTGCTG TTTTTTGCTG 
TTTGAATCGA TGAGAATGCA CATGAAATTC CAGTTGGAAA GCTATTTGAA GAAGCTTCAA 
TCGATTGTGC TCACCGAGGA AAAGCAGCAT GAAAACGGTG GTGGTGGAAC GGAGCAAAAA 
GAAATGGCTT TGGAGTCACT TGTTCAGTTG TGGAGAATTC CCGGATTGGT TACCGAGATG 
TATCTCAATT TTGATTGTGA TCTCTATTGC GGAAATATCT TCGAAGATTT GACAAAGCTT 
CTCGTTGAGA ACTCATTTCC AACAGTTGGT GGACACACAG CCTCGCTGCT CTCTCTGGAT 
GCTCTTTTAG TCGTTATTGA GACTATTGAA CAGAATTGTG AAGATCGAGA GAATGGACGA 
GGAGAAGTTG CCAAAGAGCA AGAGCACAAG GACTTAAAGA AGCTCGGGCT CCCAGTGCTC 
AGCGGCTATG ATCTAGCGAA GAAAATGGCG ATTTCAACGG GAGGAAAAGC TTCTCCAATG 
CCTGTGAGCT CATCAATTGT GCTCCGTTCG AATCGACATG CTCCGTCAAC AGAACTCCCA 
TCGATGAGCC AAATAATTGA ACAAAAGAAG CGAAAACGAC TGATTGCCGA AGGAACCGAA 
CTGTTCAATC AAAGTCCCAA AAAAGGAATC GCTTTTCTCC GGGAAAAGGG AATTCTGGGA 
CACGATGAGC AGAGCCTTGT GCAGTGGCTC CGAACGAATC CACAGTTGGA TAAGAAGGCT 
ATTGCAGATT ATATTTGTAA TCGGAAGCAT GCTGAGGTTC TCAACGCGTT TGTCAAGAGT 
TTTCCATTCG AGAATACCCG CCTGGACGTT GCTCTCCGAA TGTTCCTCGA AACGTTCCGC 
CTGCCTGGAG AATCGGCTGA AATTGCACTT GTAATGCAAC ATTTCTCCGA AGAATGGTTC 
CGAGCCAACA ATGAGCCATT TTTCCATGTC GATGCCGCTT TTACTCTCTC TTATGCGATT 
ATTATGCTCA ATGTTGATCA GCATAATCCG CAGGCCAAAC GAAGTCAACC ACCAATGACA 
GTCGACTGTT TCCGTCGGAA TCTCTCTGGA ACCAACGATT CCCGAGATTT CGACCCAGAA 
ATGCTCGCCG ACATGTACCA GGCCATCAAA ACCGAGGAAA TCGTGATGCC AGCCGAGCAA 
AAGGGCACCG TAAAGGAGGA TTACATGTGG AAAGTGTTGC TCCGTCGTGG TGAGACTGCC 
GAAGGATCGT TTTATCATGC TCCAACCGGA TGGAATGATC ATGATCTATT CGCAGTTTGC 
TGGGGTCCTG CTGTCGCTGC TCTCAGCTAT GTATTCGATA AATCGGAGCA CGAGCAGATA 
CTTCAGAAGG CTCTGACAGG CTATCGGAAG TGTGCGAAAA TCGCTGCCTA CTACGGGATG 
AAGGAGGTTT TCGATAATCT ATGTATTCAT CTTTGCAAGT TCACAACGTT AACTTCAATG 
CGGGATGGTG GAGCTGGTGG TGGAGCCGAT GAGGATGTGG ATCTTTCGGC GGCTGCTCTT 
TTGAGCCATA GCTCCTCACC TGAAGCTGTC GCCCTCGCAT TTGGAGAGAA TCATAAGGCT 
CAACTTGCCA CTCGAACCCT GTTCTATTTG GTTCATGAGA ATGGGAATAT ACTCAGAGAG 
GGATGGCGCA ACTTGTTTGA GGCTCTTCTG CAGCTGTTTA GAGCACGATT GCTTCCTGCA 
GAGCTCACTG AAGTCGAGGA TTATGTTGAT GAGAAGGGAT GGGTTAATAT TCAGAGGGTT 
CATCAGAAAG AGCTCCCACA CACCCGCAAC GATTCAGGCC TTCTTTCATG GTTCGGACTC 
GGTGGAGGCG CTTCAGAAGC GGATCGCCGT AAGCCGACCC AAGAGCAACT GAGCTCGATG 
AAGCTCGCGT CACAAGTCAT TTCGGAGTGC CGTCCGTCGC AAATTGTGGC TGATAGCAAG 
TATCTGACGA GCACATCGCT CGCCGAGCTC CTCTCATCGA TTGCGGCGAA CAGTGCGCAA 
ATTGTTGAGC AAGCCGAGCC ACAGCAAAAG ACCGCAAGCC TGAGTGGAGA GGATGAGGAT 
GCGCTGGTCT TCTACCTGGA ACTAATTGTC GCCATAACTC TCGAGAACAA GGATCGTCTA 
CCTCTTGTCT GGCCTCACGT CCGCCGCCAC CTCGAATGGC TTCTGTCACC ACGTTTCGGC 
CGGTGCCCGG TGCTCGTCGA ACGGGCCGTC GTCGGGCTTT TACGTGTAGC CAATCGGAAT 
CTATTCCGTG ATAACACAGT TTCCGACGAT GTTCTTCACT CGCTGTCGAT GCTCCTGCGA 
CTTTCCCCAA AGGCGCTCTT CATTTTCAGT CGGCAAATCG CTTTTGGGCT CTACGAGCTC 
ATTCGAGCAA ACGCGGCGAA TGTGCACAAA AAGGAGCACT GGGCAGTGCT CTTTGCGTTA 
CTTGAAGCCG CTGGAGCAGC AGTTCTACCT GATGATTATG TGATGATGAC GACGACGGAA 
AAGCAGCAGC AGAGCTTGAG AGTTGGAGGG GATCAGCAGC AGCAGAGAAT GGCTTATTCG 
GATGTTGAAG GAGCATCTGG AAGAGGTGGT GGAGCACATG AAGAAAGAGC ATATACATCG 
GAGGGAGAGG AGAGACGGCG AGGCGGTTAT GACTCCAACA GTGACCTGGA GAGCCGTGTG 
GACTCTGCTG GAAGCCTACT CGGAGCCCAG AAACAACCGG CCGACTGGAT CCATCTGGAC 
CATAAAGATG CGGCAAAAGC CACCGAAGAA GCGCTCACAG CTCTCGGAGC CAACGTTGTT 
AGCAGCAAAA AGAATTTCCG TCAATTTGGA AGCCTTGTGC TCCGGAATGG GCTCGGAAGA 
CATGAGCCAG CAGCTTTTTT GAAAGTCTGT GAGTGCCTGG CATTCTTGCT CCGCGACGCT 
GTTCACGTGA CACCGGACAA CTTTGAATCG AGCCTTCAAT GCCTTCGAAC AATGGTTGAA 
GCAAGTCTGG ATGGAGGTGT CTACGCAGCT GGGCCATTGA GTGGAGATGC TCAGAATCGG 
CTGAGGAGCA ATGTTACAGA TGAGAAAGCT GTTAAGAAAC ATCATCATCA TCATCATGGG 
CATAAGAAGA AGGAATTGTG TACGGATGTT ACTGAAGATG CTGACGAGAG CCGAAATGAA 
GAGCAACAGT TGATTGGGAA TTATCAACAG ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC 
CAACTCCATT CTCAAACTCC GGCAATCTTT GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT 
GCATCGGATC TGGCGACTGT CGCGTTCATA TGGACAGATA TCTGGCGCCC GTTGCTCCAA 
GCAATTGGAC GGCTCAGCTG TGATTGCCGA CGAGGTGTCC GTGCGGCGGC GTTGACTCAT 
TTGCAGCGCG CCTTCCTGCC GGCAAATATG GCAACTCTTG GAGCCGCAGA ATGGCAATCA 
TGCTTTGGAG AAGTGCTCTT CCCATTGCTC ACTAAGCTTC TGGAGCCATT CTCTCAAATG 
GATCCGATTG GAATGGAGGA TACACGTGTT CGGACACTTC AAATCGTCGC GAAAACTCTT 
TTAAATCATC TCTCAGCCCT GTCGGCTCTT GATTCATTCC CGGATCTTTG GATGCTTCTG 
CTCGACTATA TGGAGCAATA TCTTCGAGTT GATAGCTGTG GAAATTTGAA TGAAGCAGTG 
CCGGAATCGC TGAAAAATAT GCTTCTCGTT ATGGATAGTA CGGGAATTTT CGCTGCAACA 
CCTCGGCTTT ATGATGTGAC TGTGGAGAGG CTCAACAAAT TTATGCCCGA ATTGATTAAG 
GATACGATTC CGAATCCACC ACGGCCAGGG CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG 
AAGGAGCATG CTTCTGGGCT TGAGCCACCA CCACCATCAT CCAACTCAAC AGCCGCCACT 
TCAACATCTG ATCCTTCGAT CGCTACTGCA CAATCTTCAA TTTCGACAGC TTCATCTGTT 
GTTGGACCAC TTGTTACATG TCCAGAAGAT GCTGGAATTA GTGCTCCGAT TCCAATACAA 
CATCCATTGA CTGAAGTAAT TGTTCACTCG GGACCAACAT CACCAATTGG CTCACCACCA 
CAAACTGAGC CACCAGCTTC AAGTCCCCCG CAACACCAAC ATTCGGAGCA CCAACAATAT 
GAGCAATATC GACAGCAGCA GGCAGCGGCT GCACAGCAAT ATCAGCAGTA TAATCAGAAC 
TATCCCCAAC AACAGCAGCA ACAGCAGCAA CAATATGCAT ATTCACCAGA ACACGCGGCA 
TACTATCAGC AACAATATGC ACACCAACAA CAGCAATATG CAGAGCATTA TGCCAATCAA 
TATCAGCACT ATCAACAGCA GCAACAGCAA CAACAGCAGC ACCCAGTAAA TCCAACGAGT 
CCCAGTGTTC ACGGACAGTA TTCGGTGGCG AATCCTCTTC CACTGCCGGC TCATCCAGCT 
TATCATCCGA TTGTTGCACC ATCAGTGAAC AGTGCATTCA CCCATGTGTA CACTCCGCCA 
CAAAATAATG CTCCTGCTCT TGCTCCATCT GCTCCAACAA CAACGTCAGC AGATTCTCCG 
TATTTCACCC CGATTCCCTA CAATCCGAGC CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbf-1 ( NM_001268210.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255139.1 Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.

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CloneID	OCa352774
Clone ID Related Accession (Same CDS sequence)	NM_001268210.2 , NM_001268210.1
Accession Version	NM_001268210.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC CAACTCCATT CTCAAACTCC GGCAATCTTT 
GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT GCATCGGATC TGGCGACTGT CGCGTTCATA 
TGGACAGATA TCTGGCGCCC GTTGCTCCAA GCAATTGGAC GGCTCAGCTG TGATTGCCGA 
CGAGGTGTCC GTGCGGCGGC GTTGACTCAT TTGCAGCGCG CCTTCCTGCC GGCAAATATG 
GCAACTCTTG GAGCCGCAGA ATGGCAATCA TGCTTTGGAG AAGTGCTCTT CCCATTGCTC 
ACTAAGCTTC TGGAGCCATT CTCTCAAATG GATCCGATTG GAATGGAGGA TACACGTGTT 
CGGACACTTC AAATCGTCGC GAAAACTCTT TTAAATCATC TCTCAGCCCT GTCGGCTCTT 
GATTCATTCC CGGATCTTTG GATGCTTCTG CTCGACTATA TGGAGCAATA TCTTCGAGTT 
GATAGCTGTG GAAATTTGAA TGAAGCAGTG CCGGAATCGC TGAAAAATAT GCTTCTCGTT 
ATGGATAGTA CGGGAATTTT CGCTGCAACA CCTCGGCTTT ATGATGTGAC TGTGGAGAGG 
CTCAACAAAT TTATGCCCGA ATTGATTAAG GATACGATTC CGAATCCACC ACGGCCAGGG 
CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG AAGGAGCATG CTTCTGGGCT TGAGCCACCA 
CCACCATCAT CCAACTCAAC AGCCGCCACT TCAACATCTG ATCCTTCGAT CGCTACTGCA 
CAATCTTCAA TTTCGACAGC TTCATCTGTT GTTGGACCAC TTGTTACATG TCCAGAAGAT 
GCTGGAATTA GTGCTCCGAT TCCAATACAA CATCCATTGA CTGAAGTAAT TGTTCACTCG 
GGACCAACAT CACCAATTGG CTCACCACCA CAAACTGAGC CACCAGCTTC AAGTCCCCCG 
CAACACCAAC ATTCGGAGCA CCAACAATAT GAGCAATATC GACAGCAGCA GGCAGCGGCT 
GCACAGCAAT ATCAGCAGTA TAATCAGAAC TATCCCCAAC AACAGCAGCA ACAGCAGCAA 
CAATATGCAT ATTCACCAGA ACACGCGGCA TACTATCAGC AACAATATGC ACACCAACAA 
CAGCAATATG CAGAGCATTA TGCCAATCAA TATCAGCACT ATCAACAGCA GCAACAGCAA 
CAACAGCAGC ACCCAGTAAA TCCAACGAGT CCCAGTGTTC ACGGACAGTA TTCGGTGGCG 
AATCCTCTTC CACTGCCGGC TCATCCAGCT TATCATCCGA TTGTTGCACC ATCAGTGAAC 
AGTGCATTCA CCCATGTGTA CACTCCGCCA CAAAATAATG CTCCTGCTCT TGCTCCATCT 
GCTCCAACAA CAACGTCAGC AGATTCTCCG TATTTCACCC CGATTCCCTA CAATCCGAGC 
CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255139.1
CDS	1..1458
Translation	MSLHLLDLCSQLHSQTPAIFAKWAQGASPAASDLATVAFIWTDIWRPLLQAIGRLSCDCRRGVRAAALTHLQRAFLPANMATLGAAEWQSCFGEVLFPLLTKLLEPFSQMDPIGMEDTRVRTLQIVAKTLLNHLSALSALDSFPDLWMLLLDYMEQYLRVDSCGNLNEAVPESLKNMLLVMDSTGIFAATPRLYDVTVERLNKFMPELIKDTIPNPPRPGQQQSEASEPKKEHASGLEPPPPSSNSTAATSTSDPSIATAQSSISTASSVVGPLVTCPEDAGISAPIPIQHPLTEVIVHSGPTSPIGSPPQTEPPASSPPQHQHSEHQQYEQYRQQQAAAAQQYQQYNQNYPQQQQQQQQQYAYSPEHAAYYQQQYAHQQQQYAEHYANQYQHYQQQQQQQQQHPVNPTSPSVHGQYSVANPLPLPAHPAYHPIVAPSVNSAFTHVYTPPQNNAPALAPSAPTTTSADSPYFTPIPYNPSQQEKP

Target ORF information:

RefSeq Version	NM_001268210.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (gbf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268210.1

ORF Insert Sequence:

ATGTCCCTCC ACCTGCTCGA CTTGTGCTCC CAACTCCATT CTCAAACTCC GGCAATCTTT 
GCAAAGTGGG CTCAAGGAGC ATCTCCAGCT GCATCGGATC TGGCGACTGT CGCGTTCATA 
TGGACAGATA TCTGGCGCCC GTTGCTCCAA GCAATTGGAC GGCTCAGCTG TGATTGCCGA 
CGAGGTGTCC GTGCGGCGGC GTTGACTCAT TTGCAGCGCG CCTTCCTGCC GGCAAATATG 
GCAACTCTTG GAGCCGCAGA ATGGCAATCA TGCTTTGGAG AAGTGCTCTT CCCATTGCTC 
ACTAAGCTTC TGGAGCCATT CTCTCAAATG GATCCGATTG GAATGGAGGA TACACGTGTT 
CGGACACTTC AAATCGTCGC GAAAACTCTT TTAAATCATC TCTCAGCCCT GTCGGCTCTT 
GATTCATTCC CGGATCTTTG GATGCTTCTG CTCGACTATA TGGAGCAATA TCTTCGAGTT 
GATAGCTGTG GAAATTTGAA TGAAGCAGTG CCGGAATCGC TGAAAAATAT GCTTCTCGTT 
ATGGATAGTA CGGGAATTTT CGCTGCAACA CCTCGGCTTT ATGATGTGAC TGTGGAGAGG 
CTCAACAAAT TTATGCCCGA ATTGATTAAG GATACGATTC CGAATCCACC ACGGCCAGGG 
CAGCAGCAGT CGGAAGCGTC GGAGCCGAAG AAGGAGCATG CTTCTGGGCT TGAGCCACCA 
CCACCATCAT CCAACTCAAC AGCCGCCACT TCAACATCTG ATCCTTCGAT CGCTACTGCA 
CAATCTTCAA TTTCGACAGC TTCATCTGTT GTTGGACCAC TTGTTACATG TCCAGAAGAT 
GCTGGAATTA GTGCTCCGAT TCCAATACAA CATCCATTGA CTGAAGTAAT TGTTCACTCG 
GGACCAACAT CACCAATTGG CTCACCACCA CAAACTGAGC CACCAGCTTC AAGTCCCCCG 
CAACACCAAC ATTCGGAGCA CCAACAATAT GAGCAATATC GACAGCAGCA GGCAGCGGCT 
GCACAGCAAT ATCAGCAGTA TAATCAGAAC TATCCCCAAC AACAGCAGCA ACAGCAGCAA 
CAATATGCAT ATTCACCAGA ACACGCGGCA TACTATCAGC AACAATATGC ACACCAACAA 
CAGCAATATG CAGAGCATTA TGCCAATCAA TATCAGCACT ATCAACAGCA GCAACAGCAA 
CAACAGCAGC ACCCAGTAAA TCCAACGAGT CCCAGTGTTC ACGGACAGTA TTCGGTGGCG 
AATCCTCTTC CACTGCCGGC TCATCCAGCT TATCATCCGA TTGTTGCACC ATCAGTGAAC 
AGTGCATTCA CCCATGTGTA CACTCCGCCA CAAAATAATG CTCCTGCTCT TGCTCCATCT 
GCTCCAACAA CAACGTCAGC AGATTCTCCG TATTTCACCC CGATTCCCTA CAATCCGAGC 
CAACAGGAGA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gbf-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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