GBF1 cDNA ORF clone, Gallus gallus(chicken)

The following GBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa37627 XM_015288458.2
Latest version!
Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37628 XM_015288459.2
Latest version!
Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37629 XM_015288460.2
Latest version!
Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37630 XM_015288461.2
Latest version!
Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37631 XM_015288462.1
Latest version!
Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OGa37627 XM_015288458.1 Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37628 XM_015288459.1 Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37629 XM_015288460.1 Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa37630 XM_015288461.1 Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa37627
    Clone ID Related Accession (Same CDS sequence) XM_015288458.2 , XM_015288458.1
    Accession Version XM_015288458.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGGTATTG
    CTCTTTAAGA ACCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGAGTC ATCAAAATGG
    AAAAAACAGA AGAGATCACC AAGACCTCCT CGACATGTAA CTAAGGTCTC CCCTGCAACA
    GAACAACCAG CAGCCAATGC TAGTAATGTC ACAGCAAGCG GAGTTACTTT CATAGATGCA
    CCTTCTCCCA GTTCCTCTGG AAGCTCCGAA AATATTTCAT CTGCAGTCAG CCCTGCTACA
    GACAGTGGTT TGGAAATGTC CTCACAGGCC ACCTCCAAGG AAGACCTGAC AGACTTGGAC
    CCGGTCACTT CTTTGGGGCT TCATGCAGGA ACACCTTCAA ATGAGGCCAA AGTCACTGAT
    AATCAAAACC AGCCTGAACT CCAGAATGAA AGTCTGAGGG AGGAGAAGAT CCAGTCAGCC
    TCTGTTGAAT CTATCCCTGA GGTTTTAGAG GAGTGCACTT CTGTAGCAGA ACATTCTGAC
    TCTGCCTCAG TTCATGACAT GGACTATGTA AATCCCCGAG GAGTACGTTT TACTCAGTCT
    TCCCAAAAAG AAGGGGCAGC ACTGGTCCCG TATGGATTGC CGTGTATTAG AGAACTGTTT
    CGCTTTCTTA TCTCGCTTAC CAACCCTCTT GATCGTCACA ACTCTGAGGT GATGATCCAC
    ATGGGATTGC AGCTGCTGAC TGTAGCTCTG GAGTCTGCTC CCATTGCAAA CTGCCAGTCT
    CTCCTAGGAC TTGTGAAGGA AGAGTTGTGC CGACATCTGT TTCAACTACT GAGTGTTGAA
    CGGCTCAATC TATATGCAGC ATCCCTCAGG GTGTGTTTTC TCCTCTTTGA AAGTATGAGA
    GAGCATCTGA AATTCCAGCT GGAGATGTAT ATCAAGAAGT TGATGGAGAT AATCACTGTG
    GAAAACCCAA AGATGCCCTA TGAAATGAAG GAGATGGCTT TGGAAGCCAT TGTTCAGCTG
    TGGAGGATTC CCAGCTTTGT GACTGAGCTC TACATCAACT ATGACTGTGA CTACTACTGT
    GCCAACCTCT TTGAGGAGCT CACTAAGCTG CTCTCCAAGA ATGCCTTTCC AGTTTCTGGA
    CAGTTGTATA CAGTGCACCT GCTGTCTATG GAGGCATTGC TAACAGTGAT AGATAGCACA
    GAGGCACATT GCCAGGCCAA AGTGCTGAGT AACGTACATC AACAAGAGAA GGAAGTTGCC
    AAATCAGGCC CAGAAACAAT GAACAGCACC AAAGAAATGA GCAATAATAA TGAGAGAGCA
    CACAGTGAGG GAAAATCCAC CGCTGTAGTC TCAGAGCCAG GTGGGGCATG TCCTCCCACC
    AGTGGTTGCC TTATGGCTGA TCAGATGAAG CAAGGCTGCA TGGAATTGGA AGGAGGTGGT
    GAGACAGCTG AGAAGAATAT CCCCAAGAAG CCTACTCGAT TTTCTTGCAT CCTTCCAAGC
    CCTCAGGAGC TTATGCACAT TAAGAACAAA AAGAAGCTCC TGATAACTGG CACAGAGCAG
    TTCAACCAAA AGCCAAAGAA AGGGATACAG TTCCTGCAGG AGAAGAACCT TCTTGCCACT
    CCCATCAATA ATAATGAGGT GGCCAGATGG CTACGGGAAA ATCCTCGCCT GGACAAGAAG
    ATGATTGGGG AGTTTGTGAG TGATCGTAAG AACATAGATT TACTGGAAAG CTTTGTTGGG
    ACTTTCAGCT TCCAAGGTTT AAGACTGGAT GAAGCTTTAC GACTTTATTT AGAGGCCTTC
    CGATTACCAG GAGAGGCACC TGTAATCCAG AGACTGTTGG AAGCCTTCAC AGAACATTGG
    AGGAAATCAA ATGGATCCCC ATTTGCTAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCA
    GTTATTATGC TGAACACTGA CCAGCACAAC CATAATGTCC GCAAGCAGAA TGTCCCAATG
    ACTCTGGAGG AGTTTCGGAA AAACCTGAAA GGTGTGAATG GAGGCAAGGA CTTCGAACAA
    GACATGCTGG AAGACATGTA CCATGCCATC AAAAATGATG AGATAGTGAT GCCAGAGGAA
    CAGACAGGCC TGGTGAAAGA AAACTATATC TGGAATGTCC TGCTGCATCG TGGTGCCACT
    GATGAGGGAA TATTTCTCCA TGTGCCCCCT GGAAGCTACG ACCATGATCT CTTCACCATG
    ACATGGGGGC CAACCATTGC AGCACTTTCT TATGTTTTTG ACAAGAGCTT AGAAGAAACA
    ATCATCCAGA AAGCTATCTC TGGTTTCAGG AAATGTGCAA TGATTTCTGC TCACTATGGC
    CTCAGTGATG TGTTTGACAA TCTCATAATT TCTCTCTGCA AGTTTACAGC CCTCAGCAGC
    GAGTCTATTG AGAACCTGCC GACTGTTTTT GGAAGTAATC CCAAGGCCCA TATTGCAGCA
    AAGACTGTGT TTCACTTGGC CCATCGCCAC GGTGACATTC TGCGGGAGGG CTGGAAAAAC
    ATCATGGAGG CCCTCCTGCA GCTTTTCCGA GCAGAACTGC TGCCCAAGGC CATGGTAGAG
    GTCGAAGACT TTGTGGATCC CAATGGTAAA ATCTATCTGC AGCGTGAGGA GACACCATCT
    AACCGTGGTG AATCTACAGT GCTGAGTTTT GTTAGTTGGC TCACCCTCAG TGGGACGGAG
    CAATCTGGTA TGAGAGGGCC ATCTACTGAG ACACAGGAAG CAAAGCGAGC AGCTCTGGAG
    TGCATAAAGC AATGTGATCC TGAGAAGTTG ATCACAGAAA GTAAATTCCT CCAACTTGAA
    TCTCTCCAGG AGCTGATGAA GGCTCTAATC TCTGTGACTC CTGATGAGGA GACATACGAT
    GAAGAGGATG CTGCCTTCTG CTTGGAGATG CTTCTAAGGA TTGTACTAGA AAATAGGGAC
    CGTGTGACCT GTGTCTGGCA AACTGTCCGA GACCACCTCT ATCACCTCTG TGTCAATGCA
    ATGGAGTTCT GCTTCTTAGT GGAGAGGGCA GTGGTGGGGC TGCTGAGGCT GGCGATTCGG
    CTCCTTCGTC GGGAAGAAAT CAGTGCACAG GTTCTCCTCT CATTACGTAT CCTGCTTATG
    ATGAAGCCAA ATGTGTTGTC CAGAGTTAGT CATGAGGTTG CCTATGGCCT CCATGAACTG
    CTGAAGACCA ATGCTGCCAA CATTCATTCT GGGGATGACT GGTACACTCT CTTCACCCTC
    CTGGAGTGCA TTGGGTCTGG GGTAAAGCCA CCTGCAGCCC TGCAGGTTAC AGCAAGGGCA
    GATAACGACA CAGGTGCTCA GTCAGACAGT GAAGTCTCCT CCTCCTATCA TCCAAGTGAC
    ATGAGCCTAG ACCGTGGCTA CACGTCTGAT TCAGAGGTGT ACACAGACCA TGGGAAGCCA
    GGCAAGATGC ACCGCTCGGT GACAGATGTG GATATGATGA ACAGTGGCTG GCTAGTGGTG
    GGGAAAGATG ACATCGACAC CTCTAAACCC AGTGCTGGGC TCAACAGGCT GGGGCCATCT
    CCTCTTGTCA ACCAATACAG CCTGACGGTG GGGCTGGATT TGGGCCCACA TGACACAAAG
    TCTCTCATGA AGTGTGTAGA GTCCTTGTCC TTCATTGTGC GAGATGCTGC CCACGTGACA
    CCTGAGAACT TTGAGCTCTG TGTCAAAACC ATCCGCATAT TTGTGGAGGC AAGCTTGAAT
    GGAGGCTCTA AGTCCCAGGA GAAAAGGGGG AAGAGCCACA GACATGATAG TAAAACCAGT
    CGGTTAAAAA AAAAGCCAAA GGAGAGCTCC ACGCGGCGAT CACGGGTCTT GAGCCAGCAC
    CAGGCCCACA CACACAGTGA TGAGGACGAG GATGAGAGCA TCCCTGCCAG CTACCACACT
    GTGTCTTTAC AGCTCCTGGA TCTCATGCAC ACCTTGCACA CACGAGCAGC TTCCATCTAC
    AGCTCCTGGG CAGAGGAGCA ACGCCATCTA GAGACTTCAG GGAGGAAAAT TGAAGCAGAT
    TCCCGAACAC TGTGGTCGAA CTGCTGGTGT CCTTTGCTAC AAGGTATTGC CTGGCTATGC
    TGTGATGCCC GACGCCAGGT ACGGATGCAG GCACTCACCT ACTTGCAGCG GGCCCTCCTC
    GTACATGACC TGCAGGCCCT AGATGCCCTA GAGTGGGAGT CATGCTTCAA TAAGGTGCTG
    TTCCCTCTGC TAACGAAGCT CCTTGAGAAC ATCAGCCCAG CTGACATTGG TGGAATGGAA
    GAAACTAGGA TGAGAGCTTC AACGCTGCTC TCCAAGGTGT TCCTACAGCA TCTCTCCCCC
    CTGCTCTCAC TGACCACTTT CGCTGCATTG TGGCTGACAA TCCTTGACTT CATGGACAAA
    TACATGCATG CTGGCTCCAG TGACTTACTG CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT
    ATGCTGCTTG TAATGGACAC AGCTGGGATA TTCCACAGTG CAGACTCACG GACAGGATAC
    TCTGATCTTT GGGAGATCAC CTGGGAGCGG ATTGACTGCT TCTTACCTAG GCTGCGAGAT
    GAGCTCTTCA AACAGACAGT CATCCAAGAT CCTGTTCCCA GCATACCAAT GGAACAGCAT
    CCTCAGAAGT CAATAGCATC TGCCCTGCCC CCTTCTCCTG TGGGGGATGT GAGACCAGCC
    ACCCACCCAG CTCCATCGGA GAAGCCCTCT GAACTGAGTG CTAGTGAGTC TGAGAGAGCT
    TCTGCACCTG CATCCCCCAC CATCAGCAGC TCTTCTTCTC CTTCTTTCAC AGCATCAGCG
    CCAACAAAGA CAAGCCCCGT TACAGACATA CCTCCTACAA CAGCACAGCC ACCACTTATC
    CTGCAGCCTC TTGCATCTCC CCTGCAGGTT GGGGTGCCAC CTATGACTTT GCCAATCATC
    CTCAACCCAG CTCTAATAGA AGCCACCTCT CCTGTTCCCC TCTTAGCTAC TCCACGGCCC
    ACTGACCCCA TGACAACCTC TGAAGTCAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143944.1
    CDS11..5683
    Translation

    Target ORF information:

    RefSeq Version XM_015288458.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGGTATTG
    CTCTTTAAGA ACCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGAGTC ATCAAAATGG
    AAAAAACAGA AGAGATCACC AAGACCTCCT CGACATGTAA CTAAGGTCTC CCCTGCAACA
    GAACAACCAG CAGCCAATGC TAGTAATGTC ACAGCAAGCG GAGTTACTTT CATAGATGCA
    CCTTCTCCCA GTTCCTCTGG AAGCTCCGAA AATATTTCAT CTGCAGTCAG CCCTGCTACA
    GACAGTGGTT TGGAAATGTC CTCACAGGCC ACCTCCAAGG AAGACCTGAC AGACTTGGAC
    CCGGTCACTT CTTTGGGGCT TCATGCAGGA ACACCTTCAA ATGAGGCCAA AGTCACTGAT
    AATCAAAACC AGCCTGAACT CCAGAATGAA AGTCTGAGGG AGGAGAAGAT CCAGTCAGCC
    TCTGTTGAAT CTATCCCTGA GGTTTTAGAG GAGTGCACTT CTGTAGCAGA ACATTCTGAC
    TCTGCCTCAG TTCATGACAT GGACTATGTA AATCCCCGAG GAGTACGTTT TACTCAGTCT
    TCCCAAAAAG AAGGGGCAGC ACTGGTCCCG TATGGATTGC CGTGTATTAG AGAACTGTTT
    CGCTTTCTTA TCTCGCTTAC CAACCCTCTT GATCGTCACA ACTCTGAGGT GATGATCCAC
    ATGGGATTGC AGCTGCTGAC TGTAGCTCTG GAGTCTGCTC CCATTGCAAA CTGCCAGTCT
    CTCCTAGGAC TTGTGAAGGA AGAGTTGTGC CGACATCTGT TTCAACTACT GAGTGTTGAA
    CGGCTCAATC TATATGCAGC ATCCCTCAGG GTGTGTTTTC TCCTCTTTGA AAGTATGAGA
    GAGCATCTGA AATTCCAGCT GGAGATGTAT ATCAAGAAGT TGATGGAGAT AATCACTGTG
    GAAAACCCAA AGATGCCCTA TGAAATGAAG GAGATGGCTT TGGAAGCCAT TGTTCAGCTG
    TGGAGGATTC CCAGCTTTGT GACTGAGCTC TACATCAACT ATGACTGTGA CTACTACTGT
    GCCAACCTCT TTGAGGAGCT CACTAAGCTG CTCTCCAAGA ATGCCTTTCC AGTTTCTGGA
    CAGTTGTATA CAGTGCACCT GCTGTCTATG GAGGCATTGC TAACAGTGAT AGATAGCACA
    GAGGCACATT GCCAGGCCAA AGTGCTGAGT AACGTACATC AACAAGAGAA GGAAGTTGCC
    AAATCAGGCC CAGAAACAAT GAACAGCACC AAAGAAATGA GCAATAATAA TGAGAGAGCA
    CACAGTGAGG GAAAATCCAC CGCTGTAGTC TCAGAGCCAG GTGGGGCATG TCCTCCCACC
    AGTGGTTGCC TTATGGCTGA TCAGATGAAG CAAGGCTGCA TGGAATTGGA AGGAGGTGGT
    GAGACAGCTG AGAAGAATAT CCCCAAGAAG CCTACTCGAT TTTCTTGCAT CCTTCCAAGC
    CCTCAGGAGC TTATGCACAT TAAGAACAAA AAGAAGCTCC TGATAACTGG CACAGAGCAG
    TTCAACCAAA AGCCAAAGAA AGGGATACAG TTCCTGCAGG AGAAGAACCT TCTTGCCACT
    CCCATCAATA ATAATGAGGT GGCCAGATGG CTACGGGAAA ATCCTCGCCT GGACAAGAAG
    ATGATTGGGG AGTTTGTGAG TGATCGTAAG AACATAGATT TACTGGAAAG CTTTGTTGGG
    ACTTTCAGCT TCCAAGGTTT AAGACTGGAT GAAGCTTTAC GACTTTATTT AGAGGCCTTC
    CGATTACCAG GAGAGGCACC TGTAATCCAG AGACTGTTGG AAGCCTTCAC AGAACATTGG
    AGGAAATCAA ATGGATCCCC ATTTGCTAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCA
    GTTATTATGC TGAACACTGA CCAGCACAAC CATAATGTCC GCAAGCAGAA TGTCCCAATG
    ACTCTGGAGG AGTTTCGGAA AAACCTGAAA GGTGTGAATG GAGGCAAGGA CTTCGAACAA
    GACATGCTGG AAGACATGTA CCATGCCATC AAAAATGATG AGATAGTGAT GCCAGAGGAA
    CAGACAGGCC TGGTGAAAGA AAACTATATC TGGAATGTCC TGCTGCATCG TGGTGCCACT
    GATGAGGGAA TATTTCTCCA TGTGCCCCCT GGAAGCTACG ACCATGATCT CTTCACCATG
    ACATGGGGGC CAACCATTGC AGCACTTTCT TATGTTTTTG ACAAGAGCTT AGAAGAAACA
    ATCATCCAGA AAGCTATCTC TGGTTTCAGG AAATGTGCAA TGATTTCTGC TCACTATGGC
    CTCAGTGATG TGTTTGACAA TCTCATAATT TCTCTCTGCA AGTTTACAGC CCTCAGCAGC
    GAGTCTATTG AGAACCTGCC GACTGTTTTT GGAAGTAATC CCAAGGCCCA TATTGCAGCA
    AAGACTGTGT TTCACTTGGC CCATCGCCAC GGTGACATTC TGCGGGAGGG CTGGAAAAAC
    ATCATGGAGG CCCTCCTGCA GCTTTTCCGA GCAGAACTGC TGCCCAAGGC CATGGTAGAG
    GTCGAAGACT TTGTGGATCC CAATGGTAAA ATCTATCTGC AGCGTGAGGA GACACCATCT
    AACCGTGGTG AATCTACAGT GCTGAGTTTT GTTAGTTGGC TCACCCTCAG TGGGACGGAG
    CAATCTGGTA TGAGAGGGCC ATCTACTGAG ACACAGGAAG CAAAGCGAGC AGCTCTGGAG
    TGCATAAAGC AATGTGATCC TGAGAAGTTG ATCACAGAAA GTAAATTCCT CCAACTTGAA
    TCTCTCCAGG AGCTGATGAA GGCTCTAATC TCTGTGACTC CTGATGAGGA GACATACGAT
    GAAGAGGATG CTGCCTTCTG CTTGGAGATG CTTCTAAGGA TTGTACTAGA AAATAGGGAC
    CGTGTGACCT GTGTCTGGCA AACTGTCCGA GACCACCTCT ATCACCTCTG TGTCAATGCA
    ATGGAGTTCT GCTTCTTAGT GGAGAGGGCA GTGGTGGGGC TGCTGAGGCT GGCGATTCGG
    CTCCTTCGTC GGGAAGAAAT CAGTGCACAG GTTCTCCTCT CATTACGTAT CCTGCTTATG
    ATGAAGCCAA ATGTGTTGTC CAGAGTTAGT CATGAGGTTG CCTATGGCCT CCATGAACTG
    CTGAAGACCA ATGCTGCCAA CATTCATTCT GGGGATGACT GGTACACTCT CTTCACCCTC
    CTGGAGTGCA TTGGGTCTGG GGTAAAGCCA CCTGCAGCCC TGCAGGTTAC AGCAAGGGCA
    GATAACGACA CAGGTGCTCA GTCAGACAGT GAAGTCTCCT CCTCCTATCA TCCAAGTGAC
    ATGAGCCTAG ACCGTGGCTA CACGTCTGAT TCAGAGGTGT ACACAGACCA TGGGAAGCCA
    GGCAAGATGC ACCGCTCGGT GACAGATGTG GATATGATGA ACAGTGGCTG GCTAGTGGTG
    GGGAAAGATG ACATCGACAC CTCTAAACCC AGTGCTGGGC TCAACAGGCT GGGGCCATCT
    CCTCTTGTCA ACCAATACAG CCTGACGGTG GGGCTGGATT TGGGCCCACA TGACACAAAG
    TCTCTCATGA AGTGTGTAGA GTCCTTGTCC TTCATTGTGC GAGATGCTGC CCACGTGACA
    CCTGAGAACT TTGAGCTCTG TGTCAAAACC ATCCGCATAT TTGTGGAGGC AAGCTTGAAT
    GGAGGCTCTA AGTCCCAGGA GAAAAGGGGG AAGAGCCACA GACATGATAG TAAAACCAGT
    CGGTTAAAAA AAAAGCCAAA GGAGAGCTCC ACGCGGCGAT CACGGGTCTT GAGCCAGCAC
    CAGGCCCACA CACACAGTGA TGAGGACGAG GATGAGAGCA TCCCTGCCAG CTACCACACT
    GTGTCTTTAC AGCTCCTGGA TCTCATGCAC ACCTTGCACA CACGAGCAGC TTCCATCTAC
    AGCTCCTGGG CAGAGGAGCA ACGCCATCTA GAGACTTCAG GGAGGAAAAT TGAAGCAGAT
    TCCCGAACAC TGTGGTCGAA CTGCTGGTGT CCTTTGCTAC AAGGTATTGC CTGGCTATGC
    TGTGATGCCC GACGCCAGGT ACGGATGCAG GCACTCACCT ACTTGCAGCG GGCCCTCCTC
    GTACATGACC TGCAGGCCCT AGATGCCCTA GAGTGGGAGT CATGCTTCAA TAAGGTGCTG
    TTCCCTCTGC TAACGAAGCT CCTTGAGAAC ATCAGCCCAG CTGACATTGG TGGAATGGAA
    GAAACTAGGA TGAGAGCTTC AACGCTGCTC TCCAAGGTGT TCCTACAGCA TCTCTCCCCC
    CTGCTCTCAC TGACCACTTT CGCTGCATTG TGGCTGACAA TCCTTGACTT CATGGACAAA
    TACATGCATG CTGGCTCCAG TGACTTACTG CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT
    ATGCTGCTTG TAATGGACAC AGCTGGGATA TTCCACAGTG CAGACTCACG GACAGGATAC
    TCTGATCTTT GGGAGATCAC CTGGGAGCGG ATTGACTGCT TCTTACCTAG GCTGCGAGAT
    GAGCTCTTCA AACAGACAGT CATCCAAGAT CCTGTTCCCA GCATACCAAT GGAACAGCAT
    CCTCAGAAGT CAATAGCATC TGCCCTGCCC CCTTCTCCTG TGGGGGATGT GAGACCAGCC
    ACCCACCCAG CTCCATCGGA GAAGCCCTCT GAACTGAGTG CTAGTGAGTC TGAGAGAGCT
    TCTGCACCTG CATCCCCCAC CATCAGCAGC TCTTCTTCTC CTTCTTTCAC AGCATCAGCG
    CCAACAAAGA CAAGCCCCGT TACAGACATA CCTCCTACAA CAGCACAGCC ACCACTTATC
    CTGCAGCCTC TTGCATCTCC CCTGCAGGTT GGGGTGCCAC CTATGACTTT GCCAATCATC
    CTCAACCCAG CTCTAATAGA AGCCACCTCT CCTGTTCCCC TCTTAGCTAC TCCACGGCCC
    ACTGACCCCA TGACAACCTC TGAAGTCAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37627
    Clone ID Related Accession (Same CDS sequence) XM_015288458.2 , XM_015288458.1
    Accession Version XM_015288458.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGGTATTG
    CTCTTTAAGA ACCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGAGTC ATCAAAATGG
    AAAAAACAGA AGAGATCACC AAGACCTCCT CGACATGTAA CTAAGGTCTC CCCTGCAACA
    GAACAACCAG CAGCCAATGC TAGTAATGTC ACAGCAAGCG GAGTTACTTT CATAGATGCA
    CCTTCTCCCA GTTCCTCTGG AAGCTCCGAA AATATTTCAT CTGCAGTCAG CCCTGCTACA
    GACAGTGGTT TGGAAATGTC CTCACAGGCC ACCTCCAAGG AAGACCTGAC AGACTTGGAC
    CCGGTCACTT CTTTGGGGCT TCATGCAGGA ACACCTTCAA ATGAGGCCAA AGTCACTGAT
    AATCAAAACC AGCCTGAACT CCAGAATGAA AGTCTGAGGG AGGAGAAGAT CCAGTCAGCC
    TCTGTTGAAT CTATCCCTGA GGTTTTAGAG GAGTGCACTT CTGTAGCAGA ACATTCTGAC
    TCTGCCTCAG TTCATGACAT GGACTATGTA AATCCCCGAG GAGTACGTTT TACTCAGTCT
    TCCCAAAAAG AAGGGGCAGC ACTGGTCCCG TATGGATTGC CGTGTATTAG AGAACTGTTT
    CGCTTTCTTA TCTCGCTTAC CAACCCTCTT GATCGTCACA ACTCTGAGGT GATGATCCAC
    ATGGGATTGC AGCTGCTGAC TGTAGCTCTG GAGTCTGCTC CCATTGCAAA CTGCCAGTCT
    CTCCTAGGAC TTGTGAAGGA AGAGTTGTGC CGACATCTGT TTCAACTACT GAGTGTTGAA
    CGGCTCAATC TATATGCAGC ATCCCTCAGG GTGTGTTTTC TCCTCTTTGA AAGTATGAGA
    GAGCATCTGA AATTCCAGCT GGAGATGTAT ATCAAGAAGT TGATGGAGAT AATCACTGTG
    GAAAACCCAA AGATGCCCTA TGAAATGAAG GAGATGGCTT TGGAAGCCAT TGTTCAGCTG
    TGGAGGATTC CCAGCTTTGT GACTGAGCTC TACATCAACT ATGACTGTGA CTACTACTGT
    GCCAACCTCT TTGAGGAGCT CACTAAGCTG CTCTCCAAGA ATGCCTTTCC AGTTTCTGGA
    CAGTTGTATA CAGTGCACCT GCTGTCTATG GAGGCATTGC TAACAGTGAT AGATAGCACA
    GAGGCACATT GCCAGGCCAA AGTGCTGAGT AACGTACATC AACAAGAGAA GGAAGTTGCC
    AAATCAGGCC CAGAAACAAT GAACAGCACC AAAGAAATGA GCAATAATAA TGAGAGAGCA
    CACAGTGAGG GAAAATCCAC CGCTGTAGTC TCAGAGCCAG GTGGGGCATG TCCTCCCACC
    AGTGGTTGCC TTATGGCTGA TCAGATGAAG CAAGGCTGCA TGGAATTGGA AGGAGGTGGT
    GAGACAGCTG AGAAGAATAT CCCCAAGAAG CCTACTCGAT TTTCTTGCAT CCTTCCAAGC
    CCTCAGGAGC TTATGCACAT TAAGAACAAA AAGAAGCTCC TGATAACTGG CACAGAGCAG
    TTCAACCAAA AGCCAAAGAA AGGGATACAG TTCCTGCAGG AGAAGAACCT TCTTGCCACT
    CCCATCAATA ATAATGAGGT GGCCAGATGG CTACGGGAAA ATCCTCGCCT GGACAAGAAG
    ATGATTGGGG AGTTTGTGAG TGATCGTAAG AACATAGATT TACTGGAAAG CTTTGTTGGG
    ACTTTCAGCT TCCAAGGTTT AAGACTGGAT GAAGCTTTAC GACTTTATTT AGAGGCCTTC
    CGATTACCAG GAGAGGCACC TGTAATCCAG AGACTGTTGG AAGCCTTCAC AGAACATTGG
    AGGAAATCAA ATGGATCCCC ATTTGCTAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCA
    GTTATTATGC TGAACACTGA CCAGCACAAC CATAATGTCC GCAAGCAGAA TGTCCCAATG
    ACTCTGGAGG AGTTTCGGAA AAACCTGAAA GGTGTGAATG GAGGCAAGGA CTTCGAACAA
    GACATGCTGG AAGACATGTA CCATGCCATC AAAAATGATG AGATAGTGAT GCCAGAGGAA
    CAGACAGGCC TGGTGAAAGA AAACTATATC TGGAATGTCC TGCTGCATCG TGGTGCCACT
    GATGAGGGAA TATTTCTCCA TGTGCCCCCT GGAAGCTACG ACCATGATCT CTTCACCATG
    ACATGGGGGC CAACCATTGC AGCACTTTCT TATGTTTTTG ACAAGAGCTT AGAAGAAACA
    ATCATCCAGA AAGCTATCTC TGGTTTCAGG AAATGTGCAA TGATTTCTGC TCACTATGGC
    CTCAGTGATG TGTTTGACAA TCTCATAATT TCTCTCTGCA AGTTTACAGC CCTCAGCAGC
    GAGTCTATTG AGAACCTGCC GACTGTTTTT GGAAGTAATC CCAAGGCCCA TATTGCAGCA
    AAGACTGTGT TTCACTTGGC CCATCGCCAC GGTGACATTC TGCGGGAGGG CTGGAAAAAC
    ATCATGGAGG CCCTCCTGCA GCTTTTCCGA GCAGAACTGC TGCCCAAGGC CATGGTAGAG
    GTCGAAGACT TTGTGGATCC CAATGGTAAA ATCTATCTGC AGCGTGAGGA GACACCATCT
    AACCGTGGTG AATCTACAGT GCTGAGTTTT GTTAGTTGGC TCACCCTCAG TGGGACGGAG
    CAATCTGGTA TGAGAGGGCC ATCTACTGAG ACACAGGAAG CAAAGCGAGC AGCTCTGGAG
    TGCATAAAGC AATGTGATCC TGAGAAGTTG ATCACAGAAA GTAAATTCCT CCAACTTGAA
    TCTCTCCAGG AGCTGATGAA GGCTCTAATC TCTGTGACTC CTGATGAGGA GACATACGAT
    GAAGAGGATG CTGCCTTCTG CTTGGAGATG CTTCTAAGGA TTGTACTAGA AAATAGGGAC
    CGTGTGACCT GTGTCTGGCA AACTGTCCGA GACCACCTCT ATCACCTCTG TGTCAATGCA
    ATGGAGTTCT GCTTCTTAGT GGAGAGGGCA GTGGTGGGGC TGCTGAGGCT GGCGATTCGG
    CTCCTTCGTC GGGAAGAAAT CAGTGCACAG GTTCTCCTCT CATTACGTAT CCTGCTTATG
    ATGAAGCCAA ATGTGTTGTC CAGAGTTAGT CATGAGGTTG CCTATGGCCT CCATGAACTG
    CTGAAGACCA ATGCTGCCAA CATTCATTCT GGGGATGACT GGTACACTCT CTTCACCCTC
    CTGGAGTGCA TTGGGTCTGG GGTAAAGCCA CCTGCAGCCC TGCAGGTTAC AGCAAGGGCA
    GATAACGACA CAGGTGCTCA GTCAGACAGT GAAGTCTCCT CCTCCTATCA TCCAAGTGAC
    ATGAGCCTAG ACCGTGGCTA CACGTCTGAT TCAGAGGTGT ACACAGACCA TGGGAAGCCA
    GGCAAGATGC ACCGCTCGGT GACAGATGTG GATATGATGA ACAGTGGCTG GCTAGTGGTG
    GGGAAAGATG ACATCGACAC CTCTAAACCC AGTGCTGGGC TCAACAGGCT GGGGCCATCT
    CCTCTTGTCA ACCAATACAG CCTGACGGTG GGGCTGGATT TGGGCCCACA TGACACAAAG
    TCTCTCATGA AGTGTGTAGA GTCCTTGTCC TTCATTGTGC GAGATGCTGC CCACGTGACA
    CCTGAGAACT TTGAGCTCTG TGTCAAAACC ATCCGCATAT TTGTGGAGGC AAGCTTGAAT
    GGAGGCTCTA AGTCCCAGGA GAAAAGGGGG AAGAGCCACA GACATGATAG TAAAACCAGT
    CGGTTAAAAA AAAAGCCAAA GGAGAGCTCC ACGCGGCGAT CACGGGTCTT GAGCCAGCAC
    CAGGCCCACA CACACAGTGA TGAGGACGAG GATGAGAGCA TCCCTGCCAG CTACCACACT
    GTGTCTTTAC AGCTCCTGGA TCTCATGCAC ACCTTGCACA CACGAGCAGC TTCCATCTAC
    AGCTCCTGGG CAGAGGAGCA ACGCCATCTA GAGACTTCAG GGAGGAAAAT TGAAGCAGAT
    TCCCGAACAC TGTGGTCGAA CTGCTGGTGT CCTTTGCTAC AAGGTATTGC CTGGCTATGC
    TGTGATGCCC GACGCCAGGT ACGGATGCAG GCACTCACCT ACTTGCAGCG GGCCCTCCTC
    GTACATGACC TGCAGGCCCT AGATGCCCTA GAGTGGGAGT CATGCTTCAA TAAGGTGCTG
    TTCCCTCTGC TAACGAAGCT CCTTGAGAAC ATCAGCCCAG CTGACATTGG TGGAATGGAA
    GAAACTAGGA TGAGAGCTTC AACGCTGCTC TCCAAGGTGT TCCTACAGCA TCTCTCCCCC
    CTGCTCTCAC TGACCACTTT CGCTGCATTG TGGCTGACAA TCCTTGACTT CATGGACAAA
    TACATGCATG CTGGCTCCAG TGACTTACTG CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT
    ATGCTGCTTG TAATGGACAC AGCTGGGATA TTCCACAGTG CAGACTCACG GACAGGATAC
    TCTGATCTTT GGGAGATCAC CTGGGAGCGG ATTGACTGCT TCTTACCTAG GCTGCGAGAT
    GAGCTCTTCA AACAGACAGT CATCCAAGAT CCTGTTCCCA GCATACCAAT GGAACAGCAT
    CCTCAGAAGT CAATAGCATC TGCCCTGCCC CCTTCTCCTG TGGGGGATGT GAGACCAGCC
    ACCCACCCAG CTCCATCGGA GAAGCCCTCT GAACTGAGTG CTAGTGAGTC TGAGAGAGCT
    TCTGCACCTG CATCCCCCAC CATCAGCAGC TCTTCTTCTC CTTCTTTCAC AGCATCAGCG
    CCAACAAAGA CAAGCCCCGT TACAGACATA CCTCCTACAA CAGCACAGCC ACCACTTATC
    CTGCAGCCTC TTGCATCTCC CCTGCAGGTT GGGGTGCCAC CTATGACTTT GCCAATCATC
    CTCAACCCAG CTCTAATAGA AGCCACCTCT CCTGTTCCCC TCTTAGCTAC TCCACGGCCC
    ACTGACCCCA TGACAACCTC TGAAGTCAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143944.1
    CDS11..5683
    Translation

    Target ORF information:

    RefSeq Version XM_015288458.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGGTATTG
    CTCTTTAAGA ACCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGAGTC ATCAAAATGG
    AAAAAACAGA AGAGATCACC AAGACCTCCT CGACATGTAA CTAAGGTCTC CCCTGCAACA
    GAACAACCAG CAGCCAATGC TAGTAATGTC ACAGCAAGCG GAGTTACTTT CATAGATGCA
    CCTTCTCCCA GTTCCTCTGG AAGCTCCGAA AATATTTCAT CTGCAGTCAG CCCTGCTACA
    GACAGTGGTT TGGAAATGTC CTCACAGGCC ACCTCCAAGG AAGACCTGAC AGACTTGGAC
    CCGGTCACTT CTTTGGGGCT TCATGCAGGA ACACCTTCAA ATGAGGCCAA AGTCACTGAT
    AATCAAAACC AGCCTGAACT CCAGAATGAA AGTCTGAGGG AGGAGAAGAT CCAGTCAGCC
    TCTGTTGAAT CTATCCCTGA GGTTTTAGAG GAGTGCACTT CTGTAGCAGA ACATTCTGAC
    TCTGCCTCAG TTCATGACAT GGACTATGTA AATCCCCGAG GAGTACGTTT TACTCAGTCT
    TCCCAAAAAG AAGGGGCAGC ACTGGTCCCG TATGGATTGC CGTGTATTAG AGAACTGTTT
    CGCTTTCTTA TCTCGCTTAC CAACCCTCTT GATCGTCACA ACTCTGAGGT GATGATCCAC
    ATGGGATTGC AGCTGCTGAC TGTAGCTCTG GAGTCTGCTC CCATTGCAAA CTGCCAGTCT
    CTCCTAGGAC TTGTGAAGGA AGAGTTGTGC CGACATCTGT TTCAACTACT GAGTGTTGAA
    CGGCTCAATC TATATGCAGC ATCCCTCAGG GTGTGTTTTC TCCTCTTTGA AAGTATGAGA
    GAGCATCTGA AATTCCAGCT GGAGATGTAT ATCAAGAAGT TGATGGAGAT AATCACTGTG
    GAAAACCCAA AGATGCCCTA TGAAATGAAG GAGATGGCTT TGGAAGCCAT TGTTCAGCTG
    TGGAGGATTC CCAGCTTTGT GACTGAGCTC TACATCAACT ATGACTGTGA CTACTACTGT
    GCCAACCTCT TTGAGGAGCT CACTAAGCTG CTCTCCAAGA ATGCCTTTCC AGTTTCTGGA
    CAGTTGTATA CAGTGCACCT GCTGTCTATG GAGGCATTGC TAACAGTGAT AGATAGCACA
    GAGGCACATT GCCAGGCCAA AGTGCTGAGT AACGTACATC AACAAGAGAA GGAAGTTGCC
    AAATCAGGCC CAGAAACAAT GAACAGCACC AAAGAAATGA GCAATAATAA TGAGAGAGCA
    CACAGTGAGG GAAAATCCAC CGCTGTAGTC TCAGAGCCAG GTGGGGCATG TCCTCCCACC
    AGTGGTTGCC TTATGGCTGA TCAGATGAAG CAAGGCTGCA TGGAATTGGA AGGAGGTGGT
    GAGACAGCTG AGAAGAATAT CCCCAAGAAG CCTACTCGAT TTTCTTGCAT CCTTCCAAGC
    CCTCAGGAGC TTATGCACAT TAAGAACAAA AAGAAGCTCC TGATAACTGG CACAGAGCAG
    TTCAACCAAA AGCCAAAGAA AGGGATACAG TTCCTGCAGG AGAAGAACCT TCTTGCCACT
    CCCATCAATA ATAATGAGGT GGCCAGATGG CTACGGGAAA ATCCTCGCCT GGACAAGAAG
    ATGATTGGGG AGTTTGTGAG TGATCGTAAG AACATAGATT TACTGGAAAG CTTTGTTGGG
    ACTTTCAGCT TCCAAGGTTT AAGACTGGAT GAAGCTTTAC GACTTTATTT AGAGGCCTTC
    CGATTACCAG GAGAGGCACC TGTAATCCAG AGACTGTTGG AAGCCTTCAC AGAACATTGG
    AGGAAATCAA ATGGATCCCC ATTTGCTAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCA
    GTTATTATGC TGAACACTGA CCAGCACAAC CATAATGTCC GCAAGCAGAA TGTCCCAATG
    ACTCTGGAGG AGTTTCGGAA AAACCTGAAA GGTGTGAATG GAGGCAAGGA CTTCGAACAA
    GACATGCTGG AAGACATGTA CCATGCCATC AAAAATGATG AGATAGTGAT GCCAGAGGAA
    CAGACAGGCC TGGTGAAAGA AAACTATATC TGGAATGTCC TGCTGCATCG TGGTGCCACT
    GATGAGGGAA TATTTCTCCA TGTGCCCCCT GGAAGCTACG ACCATGATCT CTTCACCATG
    ACATGGGGGC CAACCATTGC AGCACTTTCT TATGTTTTTG ACAAGAGCTT AGAAGAAACA
    ATCATCCAGA AAGCTATCTC TGGTTTCAGG AAATGTGCAA TGATTTCTGC TCACTATGGC
    CTCAGTGATG TGTTTGACAA TCTCATAATT TCTCTCTGCA AGTTTACAGC CCTCAGCAGC
    GAGTCTATTG AGAACCTGCC GACTGTTTTT GGAAGTAATC CCAAGGCCCA TATTGCAGCA
    AAGACTGTGT TTCACTTGGC CCATCGCCAC GGTGACATTC TGCGGGAGGG CTGGAAAAAC
    ATCATGGAGG CCCTCCTGCA GCTTTTCCGA GCAGAACTGC TGCCCAAGGC CATGGTAGAG
    GTCGAAGACT TTGTGGATCC CAATGGTAAA ATCTATCTGC AGCGTGAGGA GACACCATCT
    AACCGTGGTG AATCTACAGT GCTGAGTTTT GTTAGTTGGC TCACCCTCAG TGGGACGGAG
    CAATCTGGTA TGAGAGGGCC ATCTACTGAG ACACAGGAAG CAAAGCGAGC AGCTCTGGAG
    TGCATAAAGC AATGTGATCC TGAGAAGTTG ATCACAGAAA GTAAATTCCT CCAACTTGAA
    TCTCTCCAGG AGCTGATGAA GGCTCTAATC TCTGTGACTC CTGATGAGGA GACATACGAT
    GAAGAGGATG CTGCCTTCTG CTTGGAGATG CTTCTAAGGA TTGTACTAGA AAATAGGGAC
    CGTGTGACCT GTGTCTGGCA AACTGTCCGA GACCACCTCT ATCACCTCTG TGTCAATGCA
    ATGGAGTTCT GCTTCTTAGT GGAGAGGGCA GTGGTGGGGC TGCTGAGGCT GGCGATTCGG
    CTCCTTCGTC GGGAAGAAAT CAGTGCACAG GTTCTCCTCT CATTACGTAT CCTGCTTATG
    ATGAAGCCAA ATGTGTTGTC CAGAGTTAGT CATGAGGTTG CCTATGGCCT CCATGAACTG
    CTGAAGACCA ATGCTGCCAA CATTCATTCT GGGGATGACT GGTACACTCT CTTCACCCTC
    CTGGAGTGCA TTGGGTCTGG GGTAAAGCCA CCTGCAGCCC TGCAGGTTAC AGCAAGGGCA
    GATAACGACA CAGGTGCTCA GTCAGACAGT GAAGTCTCCT CCTCCTATCA TCCAAGTGAC
    ATGAGCCTAG ACCGTGGCTA CACGTCTGAT TCAGAGGTGT ACACAGACCA TGGGAAGCCA
    GGCAAGATGC ACCGCTCGGT GACAGATGTG GATATGATGA ACAGTGGCTG GCTAGTGGTG
    GGGAAAGATG ACATCGACAC CTCTAAACCC AGTGCTGGGC TCAACAGGCT GGGGCCATCT
    CCTCTTGTCA ACCAATACAG CCTGACGGTG GGGCTGGATT TGGGCCCACA TGACACAAAG
    TCTCTCATGA AGTGTGTAGA GTCCTTGTCC TTCATTGTGC GAGATGCTGC CCACGTGACA
    CCTGAGAACT TTGAGCTCTG TGTCAAAACC ATCCGCATAT TTGTGGAGGC AAGCTTGAAT
    GGAGGCTCTA AGTCCCAGGA GAAAAGGGGG AAGAGCCACA GACATGATAG TAAAACCAGT
    CGGTTAAAAA AAAAGCCAAA GGAGAGCTCC ACGCGGCGAT CACGGGTCTT GAGCCAGCAC
    CAGGCCCACA CACACAGTGA TGAGGACGAG GATGAGAGCA TCCCTGCCAG CTACCACACT
    GTGTCTTTAC AGCTCCTGGA TCTCATGCAC ACCTTGCACA CACGAGCAGC TTCCATCTAC
    AGCTCCTGGG CAGAGGAGCA ACGCCATCTA GAGACTTCAG GGAGGAAAAT TGAAGCAGAT
    TCCCGAACAC TGTGGTCGAA CTGCTGGTGT CCTTTGCTAC AAGGTATTGC CTGGCTATGC
    TGTGATGCCC GACGCCAGGT ACGGATGCAG GCACTCACCT ACTTGCAGCG GGCCCTCCTC
    GTACATGACC TGCAGGCCCT AGATGCCCTA GAGTGGGAGT CATGCTTCAA TAAGGTGCTG
    TTCCCTCTGC TAACGAAGCT CCTTGAGAAC ATCAGCCCAG CTGACATTGG TGGAATGGAA
    GAAACTAGGA TGAGAGCTTC AACGCTGCTC TCCAAGGTGT TCCTACAGCA TCTCTCCCCC
    CTGCTCTCAC TGACCACTTT CGCTGCATTG TGGCTGACAA TCCTTGACTT CATGGACAAA
    TACATGCATG CTGGCTCCAG TGACTTACTG CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT
    ATGCTGCTTG TAATGGACAC AGCTGGGATA TTCCACAGTG CAGACTCACG GACAGGATAC
    TCTGATCTTT GGGAGATCAC CTGGGAGCGG ATTGACTGCT TCTTACCTAG GCTGCGAGAT
    GAGCTCTTCA AACAGACAGT CATCCAAGAT CCTGTTCCCA GCATACCAAT GGAACAGCAT
    CCTCAGAAGT CAATAGCATC TGCCCTGCCC CCTTCTCCTG TGGGGGATGT GAGACCAGCC
    ACCCACCCAG CTCCATCGGA GAAGCCCTCT GAACTGAGTG CTAGTGAGTC TGAGAGAGCT
    TCTGCACCTG CATCCCCCAC CATCAGCAGC TCTTCTTCTC CTTCTTTCAC AGCATCAGCG
    CCAACAAAGA CAAGCCCCGT TACAGACATA CCTCCTACAA CAGCACAGCC ACCACTTATC
    CTGCAGCCTC TTGCATCTCC CCTGCAGGTT GGGGTGCCAC CTATGACTTT GCCAATCATC
    CTCAACCCAG CTCTAATAGA AGCCACCTCT CCTGTTCCCC TCTTAGCTAC TCCACGGCCC
    ACTGACCCCA TGACAACCTC TGAAGTCAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37628
    Clone ID Related Accession (Same CDS sequence) XM_015288459.2 , XM_015288459.1
    Accession Version XM_015288459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGTTGAAA
    ATGAGAGCTG GAGGAATGAG TGAGTCATCA AAATGGAAAA AACAGAAGAG ATCACCAAGA
    CCTCCTCGAC ATGTAACTAA GGTCTCCCCT GCAACAGAAC AACCAGCAGC CAATGCTAGT
    AATGTCACAG CAAGCGGAGT TACTTTCATA GATGCACCTT CTCCCAGTTC CTCTGGAAGC
    TCCGAAAATA TTTCATCTGC AGTCAGCCCT GCTACAGACA GTGGTTTGGA AATGTCCTCA
    CAGGCCACCT CCAAGGAAGA CCTGACAGAC TTGGACCCGG TCACTTCTTT GGGGCTTCAT
    GCAGGAACAC CTTCAAATGA GGCCAAAGTC ACTGATAATC AAAACCAGCC TGAACTCCAG
    AATGAAAGTC TGAGGGAGGA GAAGATCCAG TCAGCCTCTG TTGAATCTAT CCCTGAGGTT
    TTAGAGGAGT GCACTTCTGT AGCAGAACAT TCTGACTCTG CCTCAGTTCA TGACATGGAC
    TATGTAAATC CCCGAGGAGT ACGTTTTACT CAGTCTTCCC AAAAAGAAGG GGCAGCACTG
    GTCCCGTATG GATTGCCGTG TATTAGAGAA CTGTTTCGCT TTCTTATCTC GCTTACCAAC
    CCTCTTGATC GTCACAACTC TGAGGTGATG ATCCACATGG GATTGCAGCT GCTGACTGTA
    GCTCTGGAGT CTGCTCCCAT TGCAAACTGC CAGTCTCTCC TAGGACTTGT GAAGGAAGAG
    TTGTGCCGAC ATCTGTTTCA ACTACTGAGT GTTGAACGGC TCAATCTATA TGCAGCATCC
    CTCAGGGTGT GTTTTCTCCT CTTTGAAAGT ATGAGAGAGC ATCTGAAATT CCAGCTGGAG
    ATGTATATCA AGAAGTTGAT GGAGATAATC ACTGTGGAAA ACCCAAAGAT GCCCTATGAA
    ATGAAGGAGA TGGCTTTGGA AGCCATTGTT CAGCTGTGGA GGATTCCCAG CTTTGTGACT
    GAGCTCTACA TCAACTATGA CTGTGACTAC TACTGTGCCA ACCTCTTTGA GGAGCTCACT
    AAGCTGCTCT CCAAGAATGC CTTTCCAGTT TCTGGACAGT TGTATACAGT GCACCTGCTG
    TCTATGGAGG CATTGCTAAC AGTGATAGAT AGCACAGAGG CACATTGCCA GGCCAAAGTG
    CTGAGTAACG TACATCAACA AGAGAAGGAA GTTGCCAAAT CAGGCCCAGA AACAATGAAC
    AGCACCAAAG AAATGAGCAA TAATAATGAG AGAGCACACA GTGAGGGAAA ATCCACCGCT
    GTAGTCTCAG AGCCAGGTGG GGCATGTCCT CCCACCAGTG GTTGCCTTAT GGCTGATCAG
    ATGAAGCAAG GCTGCATGGA ATTGGAAGGA GGTGGTGAGA CAGCTGAGAA GAATATCCCC
    AAGAAGCCTA CTCGATTTTC TTGCATCCTT CCAAGCCCTC AGGAGCTTAT GCACATTAAG
    AACAAAAAGA AGCTCCTGAT AACTGGCACA GAGCAGTTCA ACCAAAAGCC AAAGAAAGGG
    ATACAGTTCC TGCAGGAGAA GAACCTTCTT GCCACTCCCA TCAATAATAA TGAGGTGGCC
    AGATGGCTAC GGGAAAATCC TCGCCTGGAC AAGAAGATGA TTGGGGAGTT TGTGAGTGAT
    CGTAAGAACA TAGATTTACT GGAAAGCTTT GTTGGGACTT TCAGCTTCCA AGGTTTAAGA
    CTGGATGAAG CTTTACGACT TTATTTAGAG GCCTTCCGAT TACCAGGAGA GGCACCTGTA
    ATCCAGAGAC TGTTGGAAGC CTTCACAGAA CATTGGAGGA AATCAAATGG ATCCCCATTT
    GCTAATAGTG ATGCCTGCTT TGCCCTGGCC TATGCAGTTA TTATGCTGAA CACTGACCAG
    CACAACCATA ATGTCCGCAA GCAGAATGTC CCAATGACTC TGGAGGAGTT TCGGAAAAAC
    CTGAAAGGTG TGAATGGAGG CAAGGACTTC GAACAAGACA TGCTGGAAGA CATGTACCAT
    GCCATCAAAA ATGATGAGAT AGTGATGCCA GAGGAACAGA CAGGCCTGGT GAAAGAAAAC
    TATATCTGGA ATGTCCTGCT GCATCGTGGT GCCACTGATG AGGGAATATT TCTCCATGTG
    CCCCCTGGAA GCTACGACCA TGATCTCTTC ACCATGACAT GGGGGCCAAC CATTGCAGCA
    CTTTCTTATG TTTTTGACAA GAGCTTAGAA GAAACAATCA TCCAGAAAGC TATCTCTGGT
    TTCAGGAAAT GTGCAATGAT TTCTGCTCAC TATGGCCTCA GTGATGTGTT TGACAATCTC
    ATAATTTCTC TCTGCAAGTT TACAGCCCTC AGCAGCGAGT CTATTGAGAA CCTGCCGACT
    GTTTTTGGAA GTAATCCCAA GGCCCATATT GCAGCAAAGA CTGTGTTTCA CTTGGCCCAT
    CGCCACGGTG ACATTCTGCG GGAGGGCTGG AAAAACATCA TGGAGGCCCT CCTGCAGCTT
    TTCCGAGCAG AACTGCTGCC CAAGGCCATG GTAGAGGTCG AAGACTTTGT GGATCCCAAT
    GGTAAAATCT ATCTGCAGCG TGAGGAGACA CCATCTAACC GTGGTGAATC TACAGTGCTG
    AGTTTTGTTA GTTGGCTCAC CCTCAGTGGG ACGGAGCAAT CTGGTATGAG AGGGCCATCT
    ACTGAGACAC AGGAAGCAAA GCGAGCAGCT CTGGAGTGCA TAAAGCAATG TGATCCTGAG
    AAGTTGATCA CAGAAAGTAA ATTCCTCCAA CTTGAATCTC TCCAGGAGCT GATGAAGGCT
    CTAATCTCTG TGACTCCTGA TGAGGAGACA TACGATGAAG AGGATGCTGC CTTCTGCTTG
    GAGATGCTTC TAAGGATTGT ACTAGAAAAT AGGGACCGTG TGACCTGTGT CTGGCAAACT
    GTCCGAGACC ACCTCTATCA CCTCTGTGTC AATGCAATGG AGTTCTGCTT CTTAGTGGAG
    AGGGCAGTGG TGGGGCTGCT GAGGCTGGCG ATTCGGCTCC TTCGTCGGGA AGAAATCAGT
    GCACAGGTTC TCCTCTCATT ACGTATCCTG CTTATGATGA AGCCAAATGT GTTGTCCAGA
    GTTAGTCATG AGGTTGCCTA TGGCCTCCAT GAACTGCTGA AGACCAATGC TGCCAACATT
    CATTCTGGGG ATGACTGGTA CACTCTCTTC ACCCTCCTGG AGTGCATTGG GTCTGGGGTA
    AAGCCACCTG CAGCCCTGCA GGTTACAGCA AGGGCAGATA ACGACACAGG TGCTCAGTCA
    GACAGTGAAG TCTCCTCCTC CTATCATCCA AGTGACATGA GCCTAGACCG TGGCTACACG
    TCTGATTCAG AGGTGTACAC AGACCATGGG AAGCCAGGCA AGATGCACCG CTCGGTGACA
    GATGTGGATA TGATGAACAG TGGCTGGCTA GTGGTGGGGA AAGATGACAT CGACACCTCT
    AAACCCAGTG CTGGGCTCAA CAGGCTGGGG CCATCTCCTC TTGTCAACCA ATACAGCCTG
    ACGGTGGGGC TGGATTTGGG CCCACATGAC ACAAAGTCTC TCATGAAGTG TGTAGAGTCC
    TTGTCCTTCA TTGTGCGAGA TGCTGCCCAC GTGACACCTG AGAACTTTGA GCTCTGTGTC
    AAAACCATCC GCATATTTGT GGAGGCAAGC TTGAATGGAG GCTCTAAGTC CCAGGAGAAA
    AGGGGGAAGA GCCACAGACA TGATAGTAAA ACCAGTCGGT TAAAAAAAAA GCCAAAGGAG
    AGCTCCACGC GGCGATCACG GGTCTTGAGC CAGCACCAGG CCCACACACA CAGTGATGAG
    GACGAGGATG AGAGCATCCC TGCCAGCTAC CACACTGTGT CTTTACAGGT TAGTCAGGAC
    CTCCTGGATC TCATGCACAC CTTGCACACA CGAGCAGCTT CCATCTACAG CTCCTGGGCA
    GAGGAGCAAC GCCATCTAGA GACTTCAGGG AGGAAAATTG AAGCAGATTC CCGAACACTG
    TGGTCGAACT GCTGGTGTCC TTTGCTACAA GGTATTGCCT GGCTATGCTG TGATGCCCGA
    CGCCAGGTAC GGATGCAGGC ACTCACCTAC TTGCAGCGGG CCCTCCTCGT ACATGACCTG
    CAGGCCCTAG ATGCCCTAGA GTGGGAGTCA TGCTTCAATA AGGTGCTGTT CCCTCTGCTA
    ACGAAGCTCC TTGAGAACAT CAGCCCAGCT GACATTGGTG GAATGGAAGA AACTAGGATG
    AGAGCTTCAA CGCTGCTCTC CAAGGTGTTC CTACAGCATC TCTCCCCCCT GCTCTCACTG
    ACCACTTTCG CTGCATTGTG GCTGACAATC CTTGACTTCA TGGACAAATA CATGCATGCT
    GGCTCCAGTG ACTTACTGCT GGAGGCCATC CCAGAGTCTC TGAAGAATAT GCTGCTTGTA
    ATGGACACAG CTGGGATATT CCACAGTGCA GACTCACGGA CAGGATACTC TGATCTTTGG
    GAGATCACCT GGGAGCGGAT TGACTGCTTC TTACCTAGGC TGCGAGATGA GCTCTTCAAA
    CAGACAGTCA TCCAAGATCC TGTTCCCAGC ATACCAATGG AACAGCATCC TCAGAAGTCA
    ATAGCATCTG CCCTGCCCCC TTCTCCTGTG GGGGATGTGA GACCAGCCAC CCACCCAGCT
    CCATCGGAGA AGCCCTCTGA ACTGAGTGCT AGTGAGTCTG AGAGAGCTTC TGCACCTGCA
    TCCCCCACCA TCAGCAGCTC TTCTTCTCCT TCTTTCACAG CATCAGCGCC AACAAAGACA
    AGCCCCGTTA CAGACATACC TCCTACAACA GCACAGCCAC CACTTATCCT GCAGCCTCTT
    GCATCTCCCC TGCAGGTTGG GGTGCCACCT ATGACTTTGC CAATCATCCT CAACCCAGCT
    CTAATAGAAG CCACCTCTCC TGTTCCCCTC TTAGCTACTC CACGGCCCAC TGACCCCATG
    ACAACCTCTG AAGTCAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143945.1
    CDS11..5671
    Translation

    Target ORF information:

    RefSeq Version XM_015288459.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGTTGAAA
    ATGAGAGCTG GAGGAATGAG TGAGTCATCA AAATGGAAAA AACAGAAGAG ATCACCAAGA
    CCTCCTCGAC ATGTAACTAA GGTCTCCCCT GCAACAGAAC AACCAGCAGC CAATGCTAGT
    AATGTCACAG CAAGCGGAGT TACTTTCATA GATGCACCTT CTCCCAGTTC CTCTGGAAGC
    TCCGAAAATA TTTCATCTGC AGTCAGCCCT GCTACAGACA GTGGTTTGGA AATGTCCTCA
    CAGGCCACCT CCAAGGAAGA CCTGACAGAC TTGGACCCGG TCACTTCTTT GGGGCTTCAT
    GCAGGAACAC CTTCAAATGA GGCCAAAGTC ACTGATAATC AAAACCAGCC TGAACTCCAG
    AATGAAAGTC TGAGGGAGGA GAAGATCCAG TCAGCCTCTG TTGAATCTAT CCCTGAGGTT
    TTAGAGGAGT GCACTTCTGT AGCAGAACAT TCTGACTCTG CCTCAGTTCA TGACATGGAC
    TATGTAAATC CCCGAGGAGT ACGTTTTACT CAGTCTTCCC AAAAAGAAGG GGCAGCACTG
    GTCCCGTATG GATTGCCGTG TATTAGAGAA CTGTTTCGCT TTCTTATCTC GCTTACCAAC
    CCTCTTGATC GTCACAACTC TGAGGTGATG ATCCACATGG GATTGCAGCT GCTGACTGTA
    GCTCTGGAGT CTGCTCCCAT TGCAAACTGC CAGTCTCTCC TAGGACTTGT GAAGGAAGAG
    TTGTGCCGAC ATCTGTTTCA ACTACTGAGT GTTGAACGGC TCAATCTATA TGCAGCATCC
    CTCAGGGTGT GTTTTCTCCT CTTTGAAAGT ATGAGAGAGC ATCTGAAATT CCAGCTGGAG
    ATGTATATCA AGAAGTTGAT GGAGATAATC ACTGTGGAAA ACCCAAAGAT GCCCTATGAA
    ATGAAGGAGA TGGCTTTGGA AGCCATTGTT CAGCTGTGGA GGATTCCCAG CTTTGTGACT
    GAGCTCTACA TCAACTATGA CTGTGACTAC TACTGTGCCA ACCTCTTTGA GGAGCTCACT
    AAGCTGCTCT CCAAGAATGC CTTTCCAGTT TCTGGACAGT TGTATACAGT GCACCTGCTG
    TCTATGGAGG CATTGCTAAC AGTGATAGAT AGCACAGAGG CACATTGCCA GGCCAAAGTG
    CTGAGTAACG TACATCAACA AGAGAAGGAA GTTGCCAAAT CAGGCCCAGA AACAATGAAC
    AGCACCAAAG AAATGAGCAA TAATAATGAG AGAGCACACA GTGAGGGAAA ATCCACCGCT
    GTAGTCTCAG AGCCAGGTGG GGCATGTCCT CCCACCAGTG GTTGCCTTAT GGCTGATCAG
    ATGAAGCAAG GCTGCATGGA ATTGGAAGGA GGTGGTGAGA CAGCTGAGAA GAATATCCCC
    AAGAAGCCTA CTCGATTTTC TTGCATCCTT CCAAGCCCTC AGGAGCTTAT GCACATTAAG
    AACAAAAAGA AGCTCCTGAT AACTGGCACA GAGCAGTTCA ACCAAAAGCC AAAGAAAGGG
    ATACAGTTCC TGCAGGAGAA GAACCTTCTT GCCACTCCCA TCAATAATAA TGAGGTGGCC
    AGATGGCTAC GGGAAAATCC TCGCCTGGAC AAGAAGATGA TTGGGGAGTT TGTGAGTGAT
    CGTAAGAACA TAGATTTACT GGAAAGCTTT GTTGGGACTT TCAGCTTCCA AGGTTTAAGA
    CTGGATGAAG CTTTACGACT TTATTTAGAG GCCTTCCGAT TACCAGGAGA GGCACCTGTA
    ATCCAGAGAC TGTTGGAAGC CTTCACAGAA CATTGGAGGA AATCAAATGG ATCCCCATTT
    GCTAATAGTG ATGCCTGCTT TGCCCTGGCC TATGCAGTTA TTATGCTGAA CACTGACCAG
    CACAACCATA ATGTCCGCAA GCAGAATGTC CCAATGACTC TGGAGGAGTT TCGGAAAAAC
    CTGAAAGGTG TGAATGGAGG CAAGGACTTC GAACAAGACA TGCTGGAAGA CATGTACCAT
    GCCATCAAAA ATGATGAGAT AGTGATGCCA GAGGAACAGA CAGGCCTGGT GAAAGAAAAC
    TATATCTGGA ATGTCCTGCT GCATCGTGGT GCCACTGATG AGGGAATATT TCTCCATGTG
    CCCCCTGGAA GCTACGACCA TGATCTCTTC ACCATGACAT GGGGGCCAAC CATTGCAGCA
    CTTTCTTATG TTTTTGACAA GAGCTTAGAA GAAACAATCA TCCAGAAAGC TATCTCTGGT
    TTCAGGAAAT GTGCAATGAT TTCTGCTCAC TATGGCCTCA GTGATGTGTT TGACAATCTC
    ATAATTTCTC TCTGCAAGTT TACAGCCCTC AGCAGCGAGT CTATTGAGAA CCTGCCGACT
    GTTTTTGGAA GTAATCCCAA GGCCCATATT GCAGCAAAGA CTGTGTTTCA CTTGGCCCAT
    CGCCACGGTG ACATTCTGCG GGAGGGCTGG AAAAACATCA TGGAGGCCCT CCTGCAGCTT
    TTCCGAGCAG AACTGCTGCC CAAGGCCATG GTAGAGGTCG AAGACTTTGT GGATCCCAAT
    GGTAAAATCT ATCTGCAGCG TGAGGAGACA CCATCTAACC GTGGTGAATC TACAGTGCTG
    AGTTTTGTTA GTTGGCTCAC CCTCAGTGGG ACGGAGCAAT CTGGTATGAG AGGGCCATCT
    ACTGAGACAC AGGAAGCAAA GCGAGCAGCT CTGGAGTGCA TAAAGCAATG TGATCCTGAG
    AAGTTGATCA CAGAAAGTAA ATTCCTCCAA CTTGAATCTC TCCAGGAGCT GATGAAGGCT
    CTAATCTCTG TGACTCCTGA TGAGGAGACA TACGATGAAG AGGATGCTGC CTTCTGCTTG
    GAGATGCTTC TAAGGATTGT ACTAGAAAAT AGGGACCGTG TGACCTGTGT CTGGCAAACT
    GTCCGAGACC ACCTCTATCA CCTCTGTGTC AATGCAATGG AGTTCTGCTT CTTAGTGGAG
    AGGGCAGTGG TGGGGCTGCT GAGGCTGGCG ATTCGGCTCC TTCGTCGGGA AGAAATCAGT
    GCACAGGTTC TCCTCTCATT ACGTATCCTG CTTATGATGA AGCCAAATGT GTTGTCCAGA
    GTTAGTCATG AGGTTGCCTA TGGCCTCCAT GAACTGCTGA AGACCAATGC TGCCAACATT
    CATTCTGGGG ATGACTGGTA CACTCTCTTC ACCCTCCTGG AGTGCATTGG GTCTGGGGTA
    AAGCCACCTG CAGCCCTGCA GGTTACAGCA AGGGCAGATA ACGACACAGG TGCTCAGTCA
    GACAGTGAAG TCTCCTCCTC CTATCATCCA AGTGACATGA GCCTAGACCG TGGCTACACG
    TCTGATTCAG AGGTGTACAC AGACCATGGG AAGCCAGGCA AGATGCACCG CTCGGTGACA
    GATGTGGATA TGATGAACAG TGGCTGGCTA GTGGTGGGGA AAGATGACAT CGACACCTCT
    AAACCCAGTG CTGGGCTCAA CAGGCTGGGG CCATCTCCTC TTGTCAACCA ATACAGCCTG
    ACGGTGGGGC TGGATTTGGG CCCACATGAC ACAAAGTCTC TCATGAAGTG TGTAGAGTCC
    TTGTCCTTCA TTGTGCGAGA TGCTGCCCAC GTGACACCTG AGAACTTTGA GCTCTGTGTC
    AAAACCATCC GCATATTTGT GGAGGCAAGC TTGAATGGAG GCTCTAAGTC CCAGGAGAAA
    AGGGGGAAGA GCCACAGACA TGATAGTAAA ACCAGTCGGT TAAAAAAAAA GCCAAAGGAG
    AGCTCCACGC GGCGATCACG GGTCTTGAGC CAGCACCAGG CCCACACACA CAGTGATGAG
    GACGAGGATG AGAGCATCCC TGCCAGCTAC CACACTGTGT CTTTACAGGT TAGTCAGGAC
    CTCCTGGATC TCATGCACAC CTTGCACACA CGAGCAGCTT CCATCTACAG CTCCTGGGCA
    GAGGAGCAAC GCCATCTAGA GACTTCAGGG AGGAAAATTG AAGCAGATTC CCGAACACTG
    TGGTCGAACT GCTGGTGTCC TTTGCTACAA GGTATTGCCT GGCTATGCTG TGATGCCCGA
    CGCCAGGTAC GGATGCAGGC ACTCACCTAC TTGCAGCGGG CCCTCCTCGT ACATGACCTG
    CAGGCCCTAG ATGCCCTAGA GTGGGAGTCA TGCTTCAATA AGGTGCTGTT CCCTCTGCTA
    ACGAAGCTCC TTGAGAACAT CAGCCCAGCT GACATTGGTG GAATGGAAGA AACTAGGATG
    AGAGCTTCAA CGCTGCTCTC CAAGGTGTTC CTACAGCATC TCTCCCCCCT GCTCTCACTG
    ACCACTTTCG CTGCATTGTG GCTGACAATC CTTGACTTCA TGGACAAATA CATGCATGCT
    GGCTCCAGTG ACTTACTGCT GGAGGCCATC CCAGAGTCTC TGAAGAATAT GCTGCTTGTA
    ATGGACACAG CTGGGATATT CCACAGTGCA GACTCACGGA CAGGATACTC TGATCTTTGG
    GAGATCACCT GGGAGCGGAT TGACTGCTTC TTACCTAGGC TGCGAGATGA GCTCTTCAAA
    CAGACAGTCA TCCAAGATCC TGTTCCCAGC ATACCAATGG AACAGCATCC TCAGAAGTCA
    ATAGCATCTG CCCTGCCCCC TTCTCCTGTG GGGGATGTGA GACCAGCCAC CCACCCAGCT
    CCATCGGAGA AGCCCTCTGA ACTGAGTGCT AGTGAGTCTG AGAGAGCTTC TGCACCTGCA
    TCCCCCACCA TCAGCAGCTC TTCTTCTCCT TCTTTCACAG CATCAGCGCC AACAAAGACA
    AGCCCCGTTA CAGACATACC TCCTACAACA GCACAGCCAC CACTTATCCT GCAGCCTCTT
    GCATCTCCCC TGCAGGTTGG GGTGCCACCT ATGACTTTGC CAATCATCCT CAACCCAGCT
    CTAATAGAAG CCACCTCTCC TGTTCCCCTC TTAGCTACTC CACGGCCCAC TGACCCCATG
    ACAACCTCTG AAGTCAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37628
    Clone ID Related Accession (Same CDS sequence) XM_015288459.2 , XM_015288459.1
    Accession Version XM_015288459.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGTTGAAA
    ATGAGAGCTG GAGGAATGAG TGAGTCATCA AAATGGAAAA AACAGAAGAG ATCACCAAGA
    CCTCCTCGAC ATGTAACTAA GGTCTCCCCT GCAACAGAAC AACCAGCAGC CAATGCTAGT
    AATGTCACAG CAAGCGGAGT TACTTTCATA GATGCACCTT CTCCCAGTTC CTCTGGAAGC
    TCCGAAAATA TTTCATCTGC AGTCAGCCCT GCTACAGACA GTGGTTTGGA AATGTCCTCA
    CAGGCCACCT CCAAGGAAGA CCTGACAGAC TTGGACCCGG TCACTTCTTT GGGGCTTCAT
    GCAGGAACAC CTTCAAATGA GGCCAAAGTC ACTGATAATC AAAACCAGCC TGAACTCCAG
    AATGAAAGTC TGAGGGAGGA GAAGATCCAG TCAGCCTCTG TTGAATCTAT CCCTGAGGTT
    TTAGAGGAGT GCACTTCTGT AGCAGAACAT TCTGACTCTG CCTCAGTTCA TGACATGGAC
    TATGTAAATC CCCGAGGAGT ACGTTTTACT CAGTCTTCCC AAAAAGAAGG GGCAGCACTG
    GTCCCGTATG GATTGCCGTG TATTAGAGAA CTGTTTCGCT TTCTTATCTC GCTTACCAAC
    CCTCTTGATC GTCACAACTC TGAGGTGATG ATCCACATGG GATTGCAGCT GCTGACTGTA
    GCTCTGGAGT CTGCTCCCAT TGCAAACTGC CAGTCTCTCC TAGGACTTGT GAAGGAAGAG
    TTGTGCCGAC ATCTGTTTCA ACTACTGAGT GTTGAACGGC TCAATCTATA TGCAGCATCC
    CTCAGGGTGT GTTTTCTCCT CTTTGAAAGT ATGAGAGAGC ATCTGAAATT CCAGCTGGAG
    ATGTATATCA AGAAGTTGAT GGAGATAATC ACTGTGGAAA ACCCAAAGAT GCCCTATGAA
    ATGAAGGAGA TGGCTTTGGA AGCCATTGTT CAGCTGTGGA GGATTCCCAG CTTTGTGACT
    GAGCTCTACA TCAACTATGA CTGTGACTAC TACTGTGCCA ACCTCTTTGA GGAGCTCACT
    AAGCTGCTCT CCAAGAATGC CTTTCCAGTT TCTGGACAGT TGTATACAGT GCACCTGCTG
    TCTATGGAGG CATTGCTAAC AGTGATAGAT AGCACAGAGG CACATTGCCA GGCCAAAGTG
    CTGAGTAACG TACATCAACA AGAGAAGGAA GTTGCCAAAT CAGGCCCAGA AACAATGAAC
    AGCACCAAAG AAATGAGCAA TAATAATGAG AGAGCACACA GTGAGGGAAA ATCCACCGCT
    GTAGTCTCAG AGCCAGGTGG GGCATGTCCT CCCACCAGTG GTTGCCTTAT GGCTGATCAG
    ATGAAGCAAG GCTGCATGGA ATTGGAAGGA GGTGGTGAGA CAGCTGAGAA GAATATCCCC
    AAGAAGCCTA CTCGATTTTC TTGCATCCTT CCAAGCCCTC AGGAGCTTAT GCACATTAAG
    AACAAAAAGA AGCTCCTGAT AACTGGCACA GAGCAGTTCA ACCAAAAGCC AAAGAAAGGG
    ATACAGTTCC TGCAGGAGAA GAACCTTCTT GCCACTCCCA TCAATAATAA TGAGGTGGCC
    AGATGGCTAC GGGAAAATCC TCGCCTGGAC AAGAAGATGA TTGGGGAGTT TGTGAGTGAT
    CGTAAGAACA TAGATTTACT GGAAAGCTTT GTTGGGACTT TCAGCTTCCA AGGTTTAAGA
    CTGGATGAAG CTTTACGACT TTATTTAGAG GCCTTCCGAT TACCAGGAGA GGCACCTGTA
    ATCCAGAGAC TGTTGGAAGC CTTCACAGAA CATTGGAGGA AATCAAATGG ATCCCCATTT
    GCTAATAGTG ATGCCTGCTT TGCCCTGGCC TATGCAGTTA TTATGCTGAA CACTGACCAG
    CACAACCATA ATGTCCGCAA GCAGAATGTC CCAATGACTC TGGAGGAGTT TCGGAAAAAC
    CTGAAAGGTG TGAATGGAGG CAAGGACTTC GAACAAGACA TGCTGGAAGA CATGTACCAT
    GCCATCAAAA ATGATGAGAT AGTGATGCCA GAGGAACAGA CAGGCCTGGT GAAAGAAAAC
    TATATCTGGA ATGTCCTGCT GCATCGTGGT GCCACTGATG AGGGAATATT TCTCCATGTG
    CCCCCTGGAA GCTACGACCA TGATCTCTTC ACCATGACAT GGGGGCCAAC CATTGCAGCA
    CTTTCTTATG TTTTTGACAA GAGCTTAGAA GAAACAATCA TCCAGAAAGC TATCTCTGGT
    TTCAGGAAAT GTGCAATGAT TTCTGCTCAC TATGGCCTCA GTGATGTGTT TGACAATCTC
    ATAATTTCTC TCTGCAAGTT TACAGCCCTC AGCAGCGAGT CTATTGAGAA CCTGCCGACT
    GTTTTTGGAA GTAATCCCAA GGCCCATATT GCAGCAAAGA CTGTGTTTCA CTTGGCCCAT
    CGCCACGGTG ACATTCTGCG GGAGGGCTGG AAAAACATCA TGGAGGCCCT CCTGCAGCTT
    TTCCGAGCAG AACTGCTGCC CAAGGCCATG GTAGAGGTCG AAGACTTTGT GGATCCCAAT
    GGTAAAATCT ATCTGCAGCG TGAGGAGACA CCATCTAACC GTGGTGAATC TACAGTGCTG
    AGTTTTGTTA GTTGGCTCAC CCTCAGTGGG ACGGAGCAAT CTGGTATGAG AGGGCCATCT
    ACTGAGACAC AGGAAGCAAA GCGAGCAGCT CTGGAGTGCA TAAAGCAATG TGATCCTGAG
    AAGTTGATCA CAGAAAGTAA ATTCCTCCAA CTTGAATCTC TCCAGGAGCT GATGAAGGCT
    CTAATCTCTG TGACTCCTGA TGAGGAGACA TACGATGAAG AGGATGCTGC CTTCTGCTTG
    GAGATGCTTC TAAGGATTGT ACTAGAAAAT AGGGACCGTG TGACCTGTGT CTGGCAAACT
    GTCCGAGACC ACCTCTATCA CCTCTGTGTC AATGCAATGG AGTTCTGCTT CTTAGTGGAG
    AGGGCAGTGG TGGGGCTGCT GAGGCTGGCG ATTCGGCTCC TTCGTCGGGA AGAAATCAGT
    GCACAGGTTC TCCTCTCATT ACGTATCCTG CTTATGATGA AGCCAAATGT GTTGTCCAGA
    GTTAGTCATG AGGTTGCCTA TGGCCTCCAT GAACTGCTGA AGACCAATGC TGCCAACATT
    CATTCTGGGG ATGACTGGTA CACTCTCTTC ACCCTCCTGG AGTGCATTGG GTCTGGGGTA
    AAGCCACCTG CAGCCCTGCA GGTTACAGCA AGGGCAGATA ACGACACAGG TGCTCAGTCA
    GACAGTGAAG TCTCCTCCTC CTATCATCCA AGTGACATGA GCCTAGACCG TGGCTACACG
    TCTGATTCAG AGGTGTACAC AGACCATGGG AAGCCAGGCA AGATGCACCG CTCGGTGACA
    GATGTGGATA TGATGAACAG TGGCTGGCTA GTGGTGGGGA AAGATGACAT CGACACCTCT
    AAACCCAGTG CTGGGCTCAA CAGGCTGGGG CCATCTCCTC TTGTCAACCA ATACAGCCTG
    ACGGTGGGGC TGGATTTGGG CCCACATGAC ACAAAGTCTC TCATGAAGTG TGTAGAGTCC
    TTGTCCTTCA TTGTGCGAGA TGCTGCCCAC GTGACACCTG AGAACTTTGA GCTCTGTGTC
    AAAACCATCC GCATATTTGT GGAGGCAAGC TTGAATGGAG GCTCTAAGTC CCAGGAGAAA
    AGGGGGAAGA GCCACAGACA TGATAGTAAA ACCAGTCGGT TAAAAAAAAA GCCAAAGGAG
    AGCTCCACGC GGCGATCACG GGTCTTGAGC CAGCACCAGG CCCACACACA CAGTGATGAG
    GACGAGGATG AGAGCATCCC TGCCAGCTAC CACACTGTGT CTTTACAGGT TAGTCAGGAC
    CTCCTGGATC TCATGCACAC CTTGCACACA CGAGCAGCTT CCATCTACAG CTCCTGGGCA
    GAGGAGCAAC GCCATCTAGA GACTTCAGGG AGGAAAATTG AAGCAGATTC CCGAACACTG
    TGGTCGAACT GCTGGTGTCC TTTGCTACAA GGTATTGCCT GGCTATGCTG TGATGCCCGA
    CGCCAGGTAC GGATGCAGGC ACTCACCTAC TTGCAGCGGG CCCTCCTCGT ACATGACCTG
    CAGGCCCTAG ATGCCCTAGA GTGGGAGTCA TGCTTCAATA AGGTGCTGTT CCCTCTGCTA
    ACGAAGCTCC TTGAGAACAT CAGCCCAGCT GACATTGGTG GAATGGAAGA AACTAGGATG
    AGAGCTTCAA CGCTGCTCTC CAAGGTGTTC CTACAGCATC TCTCCCCCCT GCTCTCACTG
    ACCACTTTCG CTGCATTGTG GCTGACAATC CTTGACTTCA TGGACAAATA CATGCATGCT
    GGCTCCAGTG ACTTACTGCT GGAGGCCATC CCAGAGTCTC TGAAGAATAT GCTGCTTGTA
    ATGGACACAG CTGGGATATT CCACAGTGCA GACTCACGGA CAGGATACTC TGATCTTTGG
    GAGATCACCT GGGAGCGGAT TGACTGCTTC TTACCTAGGC TGCGAGATGA GCTCTTCAAA
    CAGACAGTCA TCCAAGATCC TGTTCCCAGC ATACCAATGG AACAGCATCC TCAGAAGTCA
    ATAGCATCTG CCCTGCCCCC TTCTCCTGTG GGGGATGTGA GACCAGCCAC CCACCCAGCT
    CCATCGGAGA AGCCCTCTGA ACTGAGTGCT AGTGAGTCTG AGAGAGCTTC TGCACCTGCA
    TCCCCCACCA TCAGCAGCTC TTCTTCTCCT TCTTTCACAG CATCAGCGCC AACAAAGACA
    AGCCCCGTTA CAGACATACC TCCTACAACA GCACAGCCAC CACTTATCCT GCAGCCTCTT
    GCATCTCCCC TGCAGGTTGG GGTGCCACCT ATGACTTTGC CAATCATCCT CAACCCAGCT
    CTAATAGAAG CCACCTCTCC TGTTCCCCTC TTAGCTACTC CACGGCCCAC TGACCCCATG
    ACAACCTCTG AAGTCAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143945.1
    CDS11..5671
    Translation

    Target ORF information:

    RefSeq Version XM_015288459.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGA TCTCTTCATG TCTCCTCAAC
    AAACTGGAAC TAAGTAGTGG GGAACAGCCC AAAGCCCTGA ACCAATTAGA GAGGTTGAAA
    ATGAGAGCTG GAGGAATGAG TGAGTCATCA AAATGGAAAA AACAGAAGAG ATCACCAAGA
    CCTCCTCGAC ATGTAACTAA GGTCTCCCCT GCAACAGAAC AACCAGCAGC CAATGCTAGT
    AATGTCACAG CAAGCGGAGT TACTTTCATA GATGCACCTT CTCCCAGTTC CTCTGGAAGC
    TCCGAAAATA TTTCATCTGC AGTCAGCCCT GCTACAGACA GTGGTTTGGA AATGTCCTCA
    CAGGCCACCT CCAAGGAAGA CCTGACAGAC TTGGACCCGG TCACTTCTTT GGGGCTTCAT
    GCAGGAACAC CTTCAAATGA GGCCAAAGTC ACTGATAATC AAAACCAGCC TGAACTCCAG
    AATGAAAGTC TGAGGGAGGA GAAGATCCAG TCAGCCTCTG TTGAATCTAT CCCTGAGGTT
    TTAGAGGAGT GCACTTCTGT AGCAGAACAT TCTGACTCTG CCTCAGTTCA TGACATGGAC
    TATGTAAATC CCCGAGGAGT ACGTTTTACT CAGTCTTCCC AAAAAGAAGG GGCAGCACTG
    GTCCCGTATG GATTGCCGTG TATTAGAGAA CTGTTTCGCT TTCTTATCTC GCTTACCAAC
    CCTCTTGATC GTCACAACTC TGAGGTGATG ATCCACATGG GATTGCAGCT GCTGACTGTA
    GCTCTGGAGT CTGCTCCCAT TGCAAACTGC CAGTCTCTCC TAGGACTTGT GAAGGAAGAG
    TTGTGCCGAC ATCTGTTTCA ACTACTGAGT GTTGAACGGC TCAATCTATA TGCAGCATCC
    CTCAGGGTGT GTTTTCTCCT CTTTGAAAGT ATGAGAGAGC ATCTGAAATT CCAGCTGGAG
    ATGTATATCA AGAAGTTGAT GGAGATAATC ACTGTGGAAA ACCCAAAGAT GCCCTATGAA
    ATGAAGGAGA TGGCTTTGGA AGCCATTGTT CAGCTGTGGA GGATTCCCAG CTTTGTGACT
    GAGCTCTACA TCAACTATGA CTGTGACTAC TACTGTGCCA ACCTCTTTGA GGAGCTCACT
    AAGCTGCTCT CCAAGAATGC CTTTCCAGTT TCTGGACAGT TGTATACAGT GCACCTGCTG
    TCTATGGAGG CATTGCTAAC AGTGATAGAT AGCACAGAGG CACATTGCCA GGCCAAAGTG
    CTGAGTAACG TACATCAACA AGAGAAGGAA GTTGCCAAAT CAGGCCCAGA AACAATGAAC
    AGCACCAAAG AAATGAGCAA TAATAATGAG AGAGCACACA GTGAGGGAAA ATCCACCGCT
    GTAGTCTCAG AGCCAGGTGG GGCATGTCCT CCCACCAGTG GTTGCCTTAT GGCTGATCAG
    ATGAAGCAAG GCTGCATGGA ATTGGAAGGA GGTGGTGAGA CAGCTGAGAA GAATATCCCC
    AAGAAGCCTA CTCGATTTTC TTGCATCCTT CCAAGCCCTC AGGAGCTTAT GCACATTAAG
    AACAAAAAGA AGCTCCTGAT AACTGGCACA GAGCAGTTCA ACCAAAAGCC AAAGAAAGGG
    ATACAGTTCC TGCAGGAGAA GAACCTTCTT GCCACTCCCA TCAATAATAA TGAGGTGGCC
    AGATGGCTAC GGGAAAATCC TCGCCTGGAC AAGAAGATGA TTGGGGAGTT TGTGAGTGAT
    CGTAAGAACA TAGATTTACT GGAAAGCTTT GTTGGGACTT TCAGCTTCCA AGGTTTAAGA
    CTGGATGAAG CTTTACGACT TTATTTAGAG GCCTTCCGAT TACCAGGAGA GGCACCTGTA
    ATCCAGAGAC TGTTGGAAGC CTTCACAGAA CATTGGAGGA AATCAAATGG ATCCCCATTT
    GCTAATAGTG ATGCCTGCTT TGCCCTGGCC TATGCAGTTA TTATGCTGAA CACTGACCAG
    CACAACCATA ATGTCCGCAA GCAGAATGTC CCAATGACTC TGGAGGAGTT TCGGAAAAAC
    CTGAAAGGTG TGAATGGAGG CAAGGACTTC GAACAAGACA TGCTGGAAGA CATGTACCAT
    GCCATCAAAA ATGATGAGAT AGTGATGCCA GAGGAACAGA CAGGCCTGGT GAAAGAAAAC
    TATATCTGGA ATGTCCTGCT GCATCGTGGT GCCACTGATG AGGGAATATT TCTCCATGTG
    CCCCCTGGAA GCTACGACCA TGATCTCTTC ACCATGACAT GGGGGCCAAC CATTGCAGCA
    CTTTCTTATG TTTTTGACAA GAGCTTAGAA GAAACAATCA TCCAGAAAGC TATCTCTGGT
    TTCAGGAAAT GTGCAATGAT TTCTGCTCAC TATGGCCTCA GTGATGTGTT TGACAATCTC
    ATAATTTCTC TCTGCAAGTT TACAGCCCTC AGCAGCGAGT CTATTGAGAA CCTGCCGACT
    GTTTTTGGAA GTAATCCCAA GGCCCATATT GCAGCAAAGA CTGTGTTTCA CTTGGCCCAT
    CGCCACGGTG ACATTCTGCG GGAGGGCTGG AAAAACATCA TGGAGGCCCT CCTGCAGCTT
    TTCCGAGCAG AACTGCTGCC CAAGGCCATG GTAGAGGTCG AAGACTTTGT GGATCCCAAT
    GGTAAAATCT ATCTGCAGCG TGAGGAGACA CCATCTAACC GTGGTGAATC TACAGTGCTG
    AGTTTTGTTA GTTGGCTCAC CCTCAGTGGG ACGGAGCAAT CTGGTATGAG AGGGCCATCT
    ACTGAGACAC AGGAAGCAAA GCGAGCAGCT CTGGAGTGCA TAAAGCAATG TGATCCTGAG
    AAGTTGATCA CAGAAAGTAA ATTCCTCCAA CTTGAATCTC TCCAGGAGCT GATGAAGGCT
    CTAATCTCTG TGACTCCTGA TGAGGAGACA TACGATGAAG AGGATGCTGC CTTCTGCTTG
    GAGATGCTTC TAAGGATTGT ACTAGAAAAT AGGGACCGTG TGACCTGTGT CTGGCAAACT
    GTCCGAGACC ACCTCTATCA CCTCTGTGTC AATGCAATGG AGTTCTGCTT CTTAGTGGAG
    AGGGCAGTGG TGGGGCTGCT GAGGCTGGCG ATTCGGCTCC TTCGTCGGGA AGAAATCAGT
    GCACAGGTTC TCCTCTCATT ACGTATCCTG CTTATGATGA AGCCAAATGT GTTGTCCAGA
    GTTAGTCATG AGGTTGCCTA TGGCCTCCAT GAACTGCTGA AGACCAATGC TGCCAACATT
    CATTCTGGGG ATGACTGGTA CACTCTCTTC ACCCTCCTGG AGTGCATTGG GTCTGGGGTA
    AAGCCACCTG CAGCCCTGCA GGTTACAGCA AGGGCAGATA ACGACACAGG TGCTCAGTCA
    GACAGTGAAG TCTCCTCCTC CTATCATCCA AGTGACATGA GCCTAGACCG TGGCTACACG
    TCTGATTCAG AGGTGTACAC AGACCATGGG AAGCCAGGCA AGATGCACCG CTCGGTGACA
    GATGTGGATA TGATGAACAG TGGCTGGCTA GTGGTGGGGA AAGATGACAT CGACACCTCT
    AAACCCAGTG CTGGGCTCAA CAGGCTGGGG CCATCTCCTC TTGTCAACCA ATACAGCCTG
    ACGGTGGGGC TGGATTTGGG CCCACATGAC ACAAAGTCTC TCATGAAGTG TGTAGAGTCC
    TTGTCCTTCA TTGTGCGAGA TGCTGCCCAC GTGACACCTG AGAACTTTGA GCTCTGTGTC
    AAAACCATCC GCATATTTGT GGAGGCAAGC TTGAATGGAG GCTCTAAGTC CCAGGAGAAA
    AGGGGGAAGA GCCACAGACA TGATAGTAAA ACCAGTCGGT TAAAAAAAAA GCCAAAGGAG
    AGCTCCACGC GGCGATCACG GGTCTTGAGC CAGCACCAGG CCCACACACA CAGTGATGAG
    GACGAGGATG AGAGCATCCC TGCCAGCTAC CACACTGTGT CTTTACAGGT TAGTCAGGAC
    CTCCTGGATC TCATGCACAC CTTGCACACA CGAGCAGCTT CCATCTACAG CTCCTGGGCA
    GAGGAGCAAC GCCATCTAGA GACTTCAGGG AGGAAAATTG AAGCAGATTC CCGAACACTG
    TGGTCGAACT GCTGGTGTCC TTTGCTACAA GGTATTGCCT GGCTATGCTG TGATGCCCGA
    CGCCAGGTAC GGATGCAGGC ACTCACCTAC TTGCAGCGGG CCCTCCTCGT ACATGACCTG
    CAGGCCCTAG ATGCCCTAGA GTGGGAGTCA TGCTTCAATA AGGTGCTGTT CCCTCTGCTA
    ACGAAGCTCC TTGAGAACAT CAGCCCAGCT GACATTGGTG GAATGGAAGA AACTAGGATG
    AGAGCTTCAA CGCTGCTCTC CAAGGTGTTC CTACAGCATC TCTCCCCCCT GCTCTCACTG
    ACCACTTTCG CTGCATTGTG GCTGACAATC CTTGACTTCA TGGACAAATA CATGCATGCT
    GGCTCCAGTG ACTTACTGCT GGAGGCCATC CCAGAGTCTC TGAAGAATAT GCTGCTTGTA
    ATGGACACAG CTGGGATATT CCACAGTGCA GACTCACGGA CAGGATACTC TGATCTTTGG
    GAGATCACCT GGGAGCGGAT TGACTGCTTC TTACCTAGGC TGCGAGATGA GCTCTTCAAA
    CAGACAGTCA TCCAAGATCC TGTTCCCAGC ATACCAATGG AACAGCATCC TCAGAAGTCA
    ATAGCATCTG CCCTGCCCCC TTCTCCTGTG GGGGATGTGA GACCAGCCAC CCACCCAGCT
    CCATCGGAGA AGCCCTCTGA ACTGAGTGCT AGTGAGTCTG AGAGAGCTTC TGCACCTGCA
    TCCCCCACCA TCAGCAGCTC TTCTTCTCCT TCTTTCACAG CATCAGCGCC AACAAAGACA
    AGCCCCGTTA CAGACATACC TCCTACAACA GCACAGCCAC CACTTATCCT GCAGCCTCTT
    GCATCTCCCC TGCAGGTTGG GGTGCCACCT ATGACTTTGC CAATCATCCT CAACCCAGCT
    CTAATAGAAG CCACCTCTCC TGTTCCCCTC TTAGCTACTC CACGGCCCAC TGACCCCATG
    ACAACCTCTG AAGTCAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37629
    Clone ID Related Accession (Same CDS sequence) XM_015288460.1 , XM_015288460.2
    Accession Version XM_015288460.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5703bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG TTGTCAAGAT GGTGGATAAA AACATTTACA TTGTACAAGG CGAAATCAAT 
    GTAGTGGTGG GAGCCATCAA GCGTAATGCC CGGTGGAGCA CTCACACACA CCTGGATGAA
    GAAAGGGATC CCTTGCTGCA TAGCTTTAGT GTCTTGAAAG AAGTGCTGAA TAATATAACA
    GAGCTATCTG AAATTGAACC CAACGTCTTT CTTCGACCTT TCCTCGAAGT GATCCGTTCT
    GAAGACACTA CAGGTCCTAT AACTGGGTTG GCCCTCACCT CTGTGAATAA GTTCCTCTCA
    TATGCACTGA TAGATCCCAG CCATGAAGGC ACAGCTGAGG GAATGGAGAA CATGGCAGAT
    GCTGTCACTC ATGCCCGATT TGTGGGTACA GATCATGCGA ATGATGAAGT TGTCTTGATG
    AAAATACTAC AGGTTTTGAG AACCTTGCTG CTCACTCCTG TGGGAGCCCA CCTCACCAAT
    GAATCCGTGT GTGAGATCAT GCAGTCTTGT TTCCGCATAT GCTTTGAAAT GAGACTGAGT
    GAGTTATTGA GAAAATCTGC AGAGCACACT CTGGTCGACA TGGTGCAGCT GCTCTTCACA
    AGGTTGCCTC AGTTCAAAGA AGAGCCTAAA AGCTACATGG GAACTAACAT GAAGAAGATC
    TCTTCATGTC TCCTCAACAA ACTGGAACTA AGTAGTGGGG AACAGCCCAA AGCCCTGAAC
    CAATTAGAGA GGGTATTGCT CTTTAAGAAC CTGAAGTTGA AAATGAGAGC TGGAGGAATG
    AGTGAGTCAT CAAAATGGAA AAAACAGAAG AGATCACCAA GACCTCCTCG ACATGTAACT
    AAGGTCTCCC CTGCAACAGA ACAACCAGCA GCCAATGCTA GTAATGTCAC AGCAAGCGGA
    GTTACTTTCA TAGATGCACC TTCTCCCAGT TCCTCTGGAA GCTCCGAAAA TATTTCATCT
    GCAGTCAGCC CTGCTACAGA CAGTGGTTTG GAAATGTCCT CACAGGCCAC CTCCAAGGAA
    GACCTGACAG ACTTGGACCC GGTCACTTCT TTGGGGCTTC ATGCAGGAAC ACCTTCAAAT
    GAGGCCAAAG TCACTGATAA TCAAAACCAG CCTGAACTCC AGAATGAAAG TCTGAGGGAG
    GAGAAGATCC AGTCAGCCTC TGTTGAATCT ATCCCTGAGG TTTTAGAGGA GTGCACTTCT
    GTAGCAGAAC ATTCTGACTC TGCCTCAGTT CATGACATGG ACTATGTAAA TCCCCGAGGA
    GTACGTTTTA CTCAGTCTTC CCAAAAAGAA GGGGCAGCAC TGGTCCCGTA TGGATTGCCG
    TGTATTAGAG AACTGTTTCG CTTTCTTATC TCGCTTACCA ACCCTCTTGA TCGTCACAAC
    TCTGAGGTGA TGATCCACAT GGGATTGCAG CTGCTGACTG TAGCTCTGGA GTCTGCTCCC
    ATTGCAAACT GCCAGTCTCT CCTAGGACTT GTGAAGGAAG AGTTGTGCCG ACATCTGTTT
    CAACTACTGA GTGTTGAACG GCTCAATCTA TATGCAGCAT CCCTCAGGGT GTGTTTTCTC
    CTCTTTGAAA GTATGAGAGA GCATCTGAAA TTCCAGCTGG AGATGTATAT CAAGAAGTTG
    ATGGAGATAA TCACTGTGGA AAACCCAAAG ATGCCCTATG AAATGAAGGA GATGGCTTTG
    GAAGCCATTG TTCAGCTGTG GAGGATTCCC AGCTTTGTGA CTGAGCTCTA CATCAACTAT
    GACTGTGACT ACTACTGTGC CAACCTCTTT GAGGAGCTCA CTAAGCTGCT CTCCAAGAAT
    GCCTTTCCAG TTTCTGGACA GTTGTATACA GTGCACCTGC TGTCTATGGA GGCATTGCTA
    ACAGTGATAG ATAGCACAGA GGCACATTGC CAGGCCAAAG TGCTGAGTAA CGTACATCAA
    CAAGAGAAGG AAGTTGCCAA ATCAGGCCCA GAAACAATGA ACAGCACCAA AGAAATGAGC
    AATAATAATG AGAGAGCACA CAGTGAGGGA AAATCCACCG CTGTAGTCTC AGAGCCAGGT
    GGGGCATGTC CTCCCACCAG TGGTTGCCTT ATGGCTGATC AGATGAAGCA AGGCTGCATG
    GAATTGGAAG GAGGTGGTGA GACAGCTGAG AAGAATATCC CCAAGAAGCC TACTCGATTT
    TCTTGCATCC TTCCAAGCCC TCAGGAGCTT ATGCACATTA AGAACAAAAA GAAGCTCCTG
    ATAACTGGCA CAGAGCAGTT CAACCAAAAG CCAAAGAAAG GGATACAGTT CCTGCAGGAG
    AAGAACCTTC TTGCCACTCC CATCAATAAT AATGAGGTGG CCAGATGGCT ACGGGAAAAT
    CCTCGCCTGG ACAAGAAGAT GATTGGGGAG TTTGTGAGTG ATCGTAAGAA CATAGATTTA
    CTGGAAAGCT TTGTTGGGAC TTTCAGCTTC CAAGGTTTAA GACTGGATGA AGCTTTACGA
    CTTTATTTAG AGGCCTTCCG ATTACCAGGA GAGGCACCTG TAATCCAGAG ACTGTTGGAA
    GCCTTCACAG AACATTGGAG GAAATCAAAT GGATCCCCAT TTGCTAATAG TGATGCCTGC
    TTTGCCCTGG CCTATGCAGT TATTATGCTG AACACTGACC AGCACAACCA TAATGTCCGC
    AAGCAGAATG TCCCAATGAC TCTGGAGGAG TTTCGGAAAA ACCTGAAAGG TGTGAATGGA
    GGCAAGGACT TCGAACAAGA CATGCTGGAA GACATGTACC ATGCCATCAA AAATGATGAG
    ATAGTGATGC CAGAGGAACA GACAGGCCTG GTGAAAGAAA ACTATATCTG GAATGTCCTG
    CTGCATCGTG GTGCCACTGA TGAGGGAATA TTTCTCCATG TGCCCCCTGG AAGCTACGAC
    CATGATCTCT TCACCATGAC ATGGGGGCCA ACCATTGCAG CACTTTCTTA TGTTTTTGAC
    AAGAGCTTAG AAGAAACAAT CATCCAGAAA GCTATCTCTG GTTTCAGGAA ATGTGCAATG
    ATTTCTGCTC ACTATGGCCT CAGTGATGTG TTTGACAATC TCATAATTTC TCTCTGCAAG
    TTTACAGCCC TCAGCAGCGA GTCTATTGAG AACCTGCCGA CTGTTTTTGG AAGTAATCCC
    AAGGCCCATA TTGCAGCAAA GACTGTGTTT CACTTGGCCC ATCGCCACGG TGACATTCTG
    CGGGAGGGCT GGAAAAACAT CATGGAGGCC CTCCTGCAGC TTTTCCGAGC AGAACTGCTG
    CCCAAGGCCA TGGTAGAGGT CGAAGACTTT GTGGATCCCA ATGGTAAAAT CTATCTGCAG
    CGTGAGGAGA CACCATCTAA CCGTGGTGAA TCTACAGTGC TGAGTTTTGT TAGTTGGCTC
    ACCCTCAGTG GGACGGAGCA ATCTGGTATG AGAGGGCCAT CTACTGAGAC ACAGGAAGCA
    AAGCGAGCAG CTCTGGAGTG CATAAAGCAA TGTGATCCTG AGAAGTTGAT CACAGAAAGT
    AAATTCCTCC AACTTGAATC TCTCCAGGAG CTGATGAAGG CTCTAATCTC TGTGACTCCT
    GATGAGGAGA CATACGATGA AGAGGATGCT GCCTTCTGCT TGGAGATGCT TCTAAGGATT
    GTACTAGAAA ATAGGGACCG TGTGACCTGT GTCTGGCAAA CTGTCCGAGA CCACCTCTAT
    CACCTCTGTG TCAATGCAAT GGAGTTCTGC TTCTTAGTGG AGAGGGCAGT GGTGGGGCTG
    CTGAGGCTGG CGATTCGGCT CCTTCGTCGG GAAGAAATCA GTGCACAGGT TCTCCTCTCA
    TTACGTATCC TGCTTATGAT GAAGCCAAAT GTGTTGTCCA GAGTTAGTCA TGAGGTTGCC
    TATGGCCTCC ATGAACTGCT GAAGACCAAT GCTGCCAACA TTCATTCTGG GGATGACTGG
    TACACTCTCT TCACCCTCCT GGAGTGCATT GGGTCTGGGG TAAAGCCACC TGCAGCCCTG
    CAGGTTACAG CAAGGGCAGA TAACGACACA GGTGCTCAGT CAGACAGTGA AGTCTCCTCC
    TCCTATCATC CAAGTGACAT GAGCCTAGAC CGTGGCTACA CGTCTGATTC AGAGGTGTAC
    ACAGACCATG GGAAGCCAGG CAAGATGCAC CGCTCGGTGA CAGATGTGGA TATGATGAAC
    AGTGGCTGGC TAGTGGTGGG GAAAGATGAC ATCGACACCT CTAAACCCAG TGCTGGGCTC
    AACAGGCTGG GGCCATCTCC TCTTGTCAAC CAATACAGCC TGACGGTGGG GCTGGATTTG
    GGCCCACATG ACACAAAGTC TCTCATGAAG TGTGTAGAGT CCTTGTCCTT CATTGTGCGA
    GATGCTGCCC ACGTGACACC TGAGAACTTT GAGCTCTGTG TCAAAACCAT CCGCATATTT
    GTGGAGGCAA GCTTGAATGG AGGCTCTAAG TCCCAGGAGA AAAGGGGGAA GAGCCACAGA
    CATGATAGTA AAACCAGTCG GTTAAAAAAA AAGCCAAAGG AGAGCTCCAC GCGGCGATCA
    CGGGTCTTGA GCCAGCACCA GGCCCACACA CACAGTGATG AGGACGAGGA TGAGAGCATC
    CCTGCCAGCT ACCACACTGT GTCTTTACAG GTTAGTCAGG ACCTCCTGGA TCTCATGCAC
    ACCTTGCACA CACGAGCAGC TTCCATCTAC AGCTCCTGGG CAGAGGAGCA ACGCCATCTA
    GAGACTTCAG GGAGGAAAAT TGAAGCAGAT TCCCGAACAC TGTGGTCGAA CTGCTGGTGT
    CCTTTGCTAC AAGGTATTGC CTGGCTATGC TGTGATGCCC GACGCCAGGT ACGGATGCAG
    GCACTCACCT ACTTGCAGCG GGCCCTCCTC GTACATGACC TGCAGGCCCT AGATGCCCTA
    GAGTGGGAGT CATGCTTCAA TAAGGTGCTG TTCCCTCTGC TAACGAAGCT CCTTGAGAAC
    ATCAGCCCAG CTGACATTGG TGGAATGGAA GAAACTAGGA TGAGAGCTTC AACGCTGCTC
    TCCAAGGTGT TCCTACAGCA TCTCTCCCCC CTGCTCTCAC TGACCACTTT CGCTGCATTG
    TGGCTGACAA TCCTTGACTT CATGGACAAA TACATGCATG CTGGCTCCAG TGACTTACTG
    CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT ATGCTGCTTG TAATGGACAC AGCTGGGATA
    TTCCACAGTG CAGACTCACG GACAGGATAC TCTGATCTTT GGGAGATCAC CTGGGAGCGG
    ATTGACTGCT TCTTACCTAG GCTGCGAGAT GAGCTCTTCA AACAGACAGT CATCCAAGAT
    CCTGTTCCCA GCATACCAAT GGAACAGCAT CCTCAGAAGT CAATAGCATC TGCCCTGCCC
    CCTTCTCCTG TGGGGGATGT GAGACCAGCC ACCCACCCAG CTCCATCGGA GAAGCCCTCT
    GAACTGAGTG CTAGTGAGTC TGAGAGAGCT TCTGCACCTG CATCCCCCAC CATCAGCAGC
    TCTTCTTCTC CTTCTTTCAC AGCATCAGCG CCAACAAAGA CAAGCCCCGT TACAGACATA
    CCTCCTACAA CAGCACAGCC ACCACTTATC CTGCAGCCTC TTGCATCTCC CCTGCAGGTT
    GGGGTGCCAC CTATGACTTT GCCAATCATC CTCAACCCAG CTCTAATAGA AGCCACCTCT
    CCTGTTCCCC TCTTAGCTAC TCCACGGCCC ACTGACCCCA TGACAACCTC TGAAGTCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143946.1
    CDS235..5937
    Translation

    Target ORF information:

    RefSeq Version XM_015288460.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG TTGTCAAGAT GGTGGATAAA AACATTTACA TTGTACAAGG CGAAATCAAT 
    GTAGTGGTGG GAGCCATCAA GCGTAATGCC CGGTGGAGCA CTCACACACA CCTGGATGAA
    GAAAGGGATC CCTTGCTGCA TAGCTTTAGT GTCTTGAAAG AAGTGCTGAA TAATATAACA
    GAGCTATCTG AAATTGAACC CAACGTCTTT CTTCGACCTT TCCTCGAAGT GATCCGTTCT
    GAAGACACTA CAGGTCCTAT AACTGGGTTG GCCCTCACCT CTGTGAATAA GTTCCTCTCA
    TATGCACTGA TAGATCCCAG CCATGAAGGC ACAGCTGAGG GAATGGAGAA CATGGCAGAT
    GCTGTCACTC ATGCCCGATT TGTGGGTACA GATCATGCGA ATGATGAAGT TGTCTTGATG
    AAAATACTAC AGGTTTTGAG AACCTTGCTG CTCACTCCTG TGGGAGCCCA CCTCACCAAT
    GAATCCGTGT GTGAGATCAT GCAGTCTTGT TTCCGCATAT GCTTTGAAAT GAGACTGAGT
    GAGTTATTGA GAAAATCTGC AGAGCACACT CTGGTCGACA TGGTGCAGCT GCTCTTCACA
    AGGTTGCCTC AGTTCAAAGA AGAGCCTAAA AGCTACATGG GAACTAACAT GAAGAAGATC
    TCTTCATGTC TCCTCAACAA ACTGGAACTA AGTAGTGGGG AACAGCCCAA AGCCCTGAAC
    CAATTAGAGA GGGTATTGCT CTTTAAGAAC CTGAAGTTGA AAATGAGAGC TGGAGGAATG
    AGTGAGTCAT CAAAATGGAA AAAACAGAAG AGATCACCAA GACCTCCTCG ACATGTAACT
    AAGGTCTCCC CTGCAACAGA ACAACCAGCA GCCAATGCTA GTAATGTCAC AGCAAGCGGA
    GTTACTTTCA TAGATGCACC TTCTCCCAGT TCCTCTGGAA GCTCCGAAAA TATTTCATCT
    GCAGTCAGCC CTGCTACAGA CAGTGGTTTG GAAATGTCCT CACAGGCCAC CTCCAAGGAA
    GACCTGACAG ACTTGGACCC GGTCACTTCT TTGGGGCTTC ATGCAGGAAC ACCTTCAAAT
    GAGGCCAAAG TCACTGATAA TCAAAACCAG CCTGAACTCC AGAATGAAAG TCTGAGGGAG
    GAGAAGATCC AGTCAGCCTC TGTTGAATCT ATCCCTGAGG TTTTAGAGGA GTGCACTTCT
    GTAGCAGAAC ATTCTGACTC TGCCTCAGTT CATGACATGG ACTATGTAAA TCCCCGAGGA
    GTACGTTTTA CTCAGTCTTC CCAAAAAGAA GGGGCAGCAC TGGTCCCGTA TGGATTGCCG
    TGTATTAGAG AACTGTTTCG CTTTCTTATC TCGCTTACCA ACCCTCTTGA TCGTCACAAC
    TCTGAGGTGA TGATCCACAT GGGATTGCAG CTGCTGACTG TAGCTCTGGA GTCTGCTCCC
    ATTGCAAACT GCCAGTCTCT CCTAGGACTT GTGAAGGAAG AGTTGTGCCG ACATCTGTTT
    CAACTACTGA GTGTTGAACG GCTCAATCTA TATGCAGCAT CCCTCAGGGT GTGTTTTCTC
    CTCTTTGAAA GTATGAGAGA GCATCTGAAA TTCCAGCTGG AGATGTATAT CAAGAAGTTG
    ATGGAGATAA TCACTGTGGA AAACCCAAAG ATGCCCTATG AAATGAAGGA GATGGCTTTG
    GAAGCCATTG TTCAGCTGTG GAGGATTCCC AGCTTTGTGA CTGAGCTCTA CATCAACTAT
    GACTGTGACT ACTACTGTGC CAACCTCTTT GAGGAGCTCA CTAAGCTGCT CTCCAAGAAT
    GCCTTTCCAG TTTCTGGACA GTTGTATACA GTGCACCTGC TGTCTATGGA GGCATTGCTA
    ACAGTGATAG ATAGCACAGA GGCACATTGC CAGGCCAAAG TGCTGAGTAA CGTACATCAA
    CAAGAGAAGG AAGTTGCCAA ATCAGGCCCA GAAACAATGA ACAGCACCAA AGAAATGAGC
    AATAATAATG AGAGAGCACA CAGTGAGGGA AAATCCACCG CTGTAGTCTC AGAGCCAGGT
    GGGGCATGTC CTCCCACCAG TGGTTGCCTT ATGGCTGATC AGATGAAGCA AGGCTGCATG
    GAATTGGAAG GAGGTGGTGA GACAGCTGAG AAGAATATCC CCAAGAAGCC TACTCGATTT
    TCTTGCATCC TTCCAAGCCC TCAGGAGCTT ATGCACATTA AGAACAAAAA GAAGCTCCTG
    ATAACTGGCA CAGAGCAGTT CAACCAAAAG CCAAAGAAAG GGATACAGTT CCTGCAGGAG
    AAGAACCTTC TTGCCACTCC CATCAATAAT AATGAGGTGG CCAGATGGCT ACGGGAAAAT
    CCTCGCCTGG ACAAGAAGAT GATTGGGGAG TTTGTGAGTG ATCGTAAGAA CATAGATTTA
    CTGGAAAGCT TTGTTGGGAC TTTCAGCTTC CAAGGTTTAA GACTGGATGA AGCTTTACGA
    CTTTATTTAG AGGCCTTCCG ATTACCAGGA GAGGCACCTG TAATCCAGAG ACTGTTGGAA
    GCCTTCACAG AACATTGGAG GAAATCAAAT GGATCCCCAT TTGCTAATAG TGATGCCTGC
    TTTGCCCTGG CCTATGCAGT TATTATGCTG AACACTGACC AGCACAACCA TAATGTCCGC
    AAGCAGAATG TCCCAATGAC TCTGGAGGAG TTTCGGAAAA ACCTGAAAGG TGTGAATGGA
    GGCAAGGACT TCGAACAAGA CATGCTGGAA GACATGTACC ATGCCATCAA AAATGATGAG
    ATAGTGATGC CAGAGGAACA GACAGGCCTG GTGAAAGAAA ACTATATCTG GAATGTCCTG
    CTGCATCGTG GTGCCACTGA TGAGGGAATA TTTCTCCATG TGCCCCCTGG AAGCTACGAC
    CATGATCTCT TCACCATGAC ATGGGGGCCA ACCATTGCAG CACTTTCTTA TGTTTTTGAC
    AAGAGCTTAG AAGAAACAAT CATCCAGAAA GCTATCTCTG GTTTCAGGAA ATGTGCAATG
    ATTTCTGCTC ACTATGGCCT CAGTGATGTG TTTGACAATC TCATAATTTC TCTCTGCAAG
    TTTACAGCCC TCAGCAGCGA GTCTATTGAG AACCTGCCGA CTGTTTTTGG AAGTAATCCC
    AAGGCCCATA TTGCAGCAAA GACTGTGTTT CACTTGGCCC ATCGCCACGG TGACATTCTG
    CGGGAGGGCT GGAAAAACAT CATGGAGGCC CTCCTGCAGC TTTTCCGAGC AGAACTGCTG
    CCCAAGGCCA TGGTAGAGGT CGAAGACTTT GTGGATCCCA ATGGTAAAAT CTATCTGCAG
    CGTGAGGAGA CACCATCTAA CCGTGGTGAA TCTACAGTGC TGAGTTTTGT TAGTTGGCTC
    ACCCTCAGTG GGACGGAGCA ATCTGGTATG AGAGGGCCAT CTACTGAGAC ACAGGAAGCA
    AAGCGAGCAG CTCTGGAGTG CATAAAGCAA TGTGATCCTG AGAAGTTGAT CACAGAAAGT
    AAATTCCTCC AACTTGAATC TCTCCAGGAG CTGATGAAGG CTCTAATCTC TGTGACTCCT
    GATGAGGAGA CATACGATGA AGAGGATGCT GCCTTCTGCT TGGAGATGCT TCTAAGGATT
    GTACTAGAAA ATAGGGACCG TGTGACCTGT GTCTGGCAAA CTGTCCGAGA CCACCTCTAT
    CACCTCTGTG TCAATGCAAT GGAGTTCTGC TTCTTAGTGG AGAGGGCAGT GGTGGGGCTG
    CTGAGGCTGG CGATTCGGCT CCTTCGTCGG GAAGAAATCA GTGCACAGGT TCTCCTCTCA
    TTACGTATCC TGCTTATGAT GAAGCCAAAT GTGTTGTCCA GAGTTAGTCA TGAGGTTGCC
    TATGGCCTCC ATGAACTGCT GAAGACCAAT GCTGCCAACA TTCATTCTGG GGATGACTGG
    TACACTCTCT TCACCCTCCT GGAGTGCATT GGGTCTGGGG TAAAGCCACC TGCAGCCCTG
    CAGGTTACAG CAAGGGCAGA TAACGACACA GGTGCTCAGT CAGACAGTGA AGTCTCCTCC
    TCCTATCATC CAAGTGACAT GAGCCTAGAC CGTGGCTACA CGTCTGATTC AGAGGTGTAC
    ACAGACCATG GGAAGCCAGG CAAGATGCAC CGCTCGGTGA CAGATGTGGA TATGATGAAC
    AGTGGCTGGC TAGTGGTGGG GAAAGATGAC ATCGACACCT CTAAACCCAG TGCTGGGCTC
    AACAGGCTGG GGCCATCTCC TCTTGTCAAC CAATACAGCC TGACGGTGGG GCTGGATTTG
    GGCCCACATG ACACAAAGTC TCTCATGAAG TGTGTAGAGT CCTTGTCCTT CATTGTGCGA
    GATGCTGCCC ACGTGACACC TGAGAACTTT GAGCTCTGTG TCAAAACCAT CCGCATATTT
    GTGGAGGCAA GCTTGAATGG AGGCTCTAAG TCCCAGGAGA AAAGGGGGAA GAGCCACAGA
    CATGATAGTA AAACCAGTCG GTTAAAAAAA AAGCCAAAGG AGAGCTCCAC GCGGCGATCA
    CGGGTCTTGA GCCAGCACCA GGCCCACACA CACAGTGATG AGGACGAGGA TGAGAGCATC
    CCTGCCAGCT ACCACACTGT GTCTTTACAG GTTAGTCAGG ACCTCCTGGA TCTCATGCAC
    ACCTTGCACA CACGAGCAGC TTCCATCTAC AGCTCCTGGG CAGAGGAGCA ACGCCATCTA
    GAGACTTCAG GGAGGAAAAT TGAAGCAGAT TCCCGAACAC TGTGGTCGAA CTGCTGGTGT
    CCTTTGCTAC AAGGTATTGC CTGGCTATGC TGTGATGCCC GACGCCAGGT ACGGATGCAG
    GCACTCACCT ACTTGCAGCG GGCCCTCCTC GTACATGACC TGCAGGCCCT AGATGCCCTA
    GAGTGGGAGT CATGCTTCAA TAAGGTGCTG TTCCCTCTGC TAACGAAGCT CCTTGAGAAC
    ATCAGCCCAG CTGACATTGG TGGAATGGAA GAAACTAGGA TGAGAGCTTC AACGCTGCTC
    TCCAAGGTGT TCCTACAGCA TCTCTCCCCC CTGCTCTCAC TGACCACTTT CGCTGCATTG
    TGGCTGACAA TCCTTGACTT CATGGACAAA TACATGCATG CTGGCTCCAG TGACTTACTG
    CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT ATGCTGCTTG TAATGGACAC AGCTGGGATA
    TTCCACAGTG CAGACTCACG GACAGGATAC TCTGATCTTT GGGAGATCAC CTGGGAGCGG
    ATTGACTGCT TCTTACCTAG GCTGCGAGAT GAGCTCTTCA AACAGACAGT CATCCAAGAT
    CCTGTTCCCA GCATACCAAT GGAACAGCAT CCTCAGAAGT CAATAGCATC TGCCCTGCCC
    CCTTCTCCTG TGGGGGATGT GAGACCAGCC ACCCACCCAG CTCCATCGGA GAAGCCCTCT
    GAACTGAGTG CTAGTGAGTC TGAGAGAGCT TCTGCACCTG CATCCCCCAC CATCAGCAGC
    TCTTCTTCTC CTTCTTTCAC AGCATCAGCG CCAACAAAGA CAAGCCCCGT TACAGACATA
    CCTCCTACAA CAGCACAGCC ACCACTTATC CTGCAGCCTC TTGCATCTCC CCTGCAGGTT
    GGGGTGCCAC CTATGACTTT GCCAATCATC CTCAACCCAG CTCTAATAGA AGCCACCTCT
    CCTGTTCCCC TCTTAGCTAC TCCACGGCCC ACTGACCCCA TGACAACCTC TGAAGTCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37629
    Clone ID Related Accession (Same CDS sequence) XM_015288460.1 , XM_015288460.2
    Accession Version XM_015288460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5703bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG TTGTCAAGAT GGTGGATAAA AACATTTACA TTGTACAAGG CGAAATCAAT 
    GTAGTGGTGG GAGCCATCAA GCGTAATGCC CGGTGGAGCA CTCACACACA CCTGGATGAA
    GAAAGGGATC CCTTGCTGCA TAGCTTTAGT GTCTTGAAAG AAGTGCTGAA TAATATAACA
    GAGCTATCTG AAATTGAACC CAACGTCTTT CTTCGACCTT TCCTCGAAGT GATCCGTTCT
    GAAGACACTA CAGGTCCTAT AACTGGGTTG GCCCTCACCT CTGTGAATAA GTTCCTCTCA
    TATGCACTGA TAGATCCCAG CCATGAAGGC ACAGCTGAGG GAATGGAGAA CATGGCAGAT
    GCTGTCACTC ATGCCCGATT TGTGGGTACA GATCATGCGA ATGATGAAGT TGTCTTGATG
    AAAATACTAC AGGTTTTGAG AACCTTGCTG CTCACTCCTG TGGGAGCCCA CCTCACCAAT
    GAATCCGTGT GTGAGATCAT GCAGTCTTGT TTCCGCATAT GCTTTGAAAT GAGACTGAGT
    GAGTTATTGA GAAAATCTGC AGAGCACACT CTGGTCGACA TGGTGCAGCT GCTCTTCACA
    AGGTTGCCTC AGTTCAAAGA AGAGCCTAAA AGCTACATGG GAACTAACAT GAAGAAGATC
    TCTTCATGTC TCCTCAACAA ACTGGAACTA AGTAGTGGGG AACAGCCCAA AGCCCTGAAC
    CAATTAGAGA GGGTATTGCT CTTTAAGAAC CTGAAGTTGA AAATGAGAGC TGGAGGAATG
    AGTGAGTCAT CAAAATGGAA AAAACAGAAG AGATCACCAA GACCTCCTCG ACATGTAACT
    AAGGTCTCCC CTGCAACAGA ACAACCAGCA GCCAATGCTA GTAATGTCAC AGCAAGCGGA
    GTTACTTTCA TAGATGCACC TTCTCCCAGT TCCTCTGGAA GCTCCGAAAA TATTTCATCT
    GCAGTCAGCC CTGCTACAGA CAGTGGTTTG GAAATGTCCT CACAGGCCAC CTCCAAGGAA
    GACCTGACAG ACTTGGACCC GGTCACTTCT TTGGGGCTTC ATGCAGGAAC ACCTTCAAAT
    GAGGCCAAAG TCACTGATAA TCAAAACCAG CCTGAACTCC AGAATGAAAG TCTGAGGGAG
    GAGAAGATCC AGTCAGCCTC TGTTGAATCT ATCCCTGAGG TTTTAGAGGA GTGCACTTCT
    GTAGCAGAAC ATTCTGACTC TGCCTCAGTT CATGACATGG ACTATGTAAA TCCCCGAGGA
    GTACGTTTTA CTCAGTCTTC CCAAAAAGAA GGGGCAGCAC TGGTCCCGTA TGGATTGCCG
    TGTATTAGAG AACTGTTTCG CTTTCTTATC TCGCTTACCA ACCCTCTTGA TCGTCACAAC
    TCTGAGGTGA TGATCCACAT GGGATTGCAG CTGCTGACTG TAGCTCTGGA GTCTGCTCCC
    ATTGCAAACT GCCAGTCTCT CCTAGGACTT GTGAAGGAAG AGTTGTGCCG ACATCTGTTT
    CAACTACTGA GTGTTGAACG GCTCAATCTA TATGCAGCAT CCCTCAGGGT GTGTTTTCTC
    CTCTTTGAAA GTATGAGAGA GCATCTGAAA TTCCAGCTGG AGATGTATAT CAAGAAGTTG
    ATGGAGATAA TCACTGTGGA AAACCCAAAG ATGCCCTATG AAATGAAGGA GATGGCTTTG
    GAAGCCATTG TTCAGCTGTG GAGGATTCCC AGCTTTGTGA CTGAGCTCTA CATCAACTAT
    GACTGTGACT ACTACTGTGC CAACCTCTTT GAGGAGCTCA CTAAGCTGCT CTCCAAGAAT
    GCCTTTCCAG TTTCTGGACA GTTGTATACA GTGCACCTGC TGTCTATGGA GGCATTGCTA
    ACAGTGATAG ATAGCACAGA GGCACATTGC CAGGCCAAAG TGCTGAGTAA CGTACATCAA
    CAAGAGAAGG AAGTTGCCAA ATCAGGCCCA GAAACAATGA ACAGCACCAA AGAAATGAGC
    AATAATAATG AGAGAGCACA CAGTGAGGGA AAATCCACCG CTGTAGTCTC AGAGCCAGGT
    GGGGCATGTC CTCCCACCAG TGGTTGCCTT ATGGCTGATC AGATGAAGCA AGGCTGCATG
    GAATTGGAAG GAGGTGGTGA GACAGCTGAG AAGAATATCC CCAAGAAGCC TACTCGATTT
    TCTTGCATCC TTCCAAGCCC TCAGGAGCTT ATGCACATTA AGAACAAAAA GAAGCTCCTG
    ATAACTGGCA CAGAGCAGTT CAACCAAAAG CCAAAGAAAG GGATACAGTT CCTGCAGGAG
    AAGAACCTTC TTGCCACTCC CATCAATAAT AATGAGGTGG CCAGATGGCT ACGGGAAAAT
    CCTCGCCTGG ACAAGAAGAT GATTGGGGAG TTTGTGAGTG ATCGTAAGAA CATAGATTTA
    CTGGAAAGCT TTGTTGGGAC TTTCAGCTTC CAAGGTTTAA GACTGGATGA AGCTTTACGA
    CTTTATTTAG AGGCCTTCCG ATTACCAGGA GAGGCACCTG TAATCCAGAG ACTGTTGGAA
    GCCTTCACAG AACATTGGAG GAAATCAAAT GGATCCCCAT TTGCTAATAG TGATGCCTGC
    TTTGCCCTGG CCTATGCAGT TATTATGCTG AACACTGACC AGCACAACCA TAATGTCCGC
    AAGCAGAATG TCCCAATGAC TCTGGAGGAG TTTCGGAAAA ACCTGAAAGG TGTGAATGGA
    GGCAAGGACT TCGAACAAGA CATGCTGGAA GACATGTACC ATGCCATCAA AAATGATGAG
    ATAGTGATGC CAGAGGAACA GACAGGCCTG GTGAAAGAAA ACTATATCTG GAATGTCCTG
    CTGCATCGTG GTGCCACTGA TGAGGGAATA TTTCTCCATG TGCCCCCTGG AAGCTACGAC
    CATGATCTCT TCACCATGAC ATGGGGGCCA ACCATTGCAG CACTTTCTTA TGTTTTTGAC
    AAGAGCTTAG AAGAAACAAT CATCCAGAAA GCTATCTCTG GTTTCAGGAA ATGTGCAATG
    ATTTCTGCTC ACTATGGCCT CAGTGATGTG TTTGACAATC TCATAATTTC TCTCTGCAAG
    TTTACAGCCC TCAGCAGCGA GTCTATTGAG AACCTGCCGA CTGTTTTTGG AAGTAATCCC
    AAGGCCCATA TTGCAGCAAA GACTGTGTTT CACTTGGCCC ATCGCCACGG TGACATTCTG
    CGGGAGGGCT GGAAAAACAT CATGGAGGCC CTCCTGCAGC TTTTCCGAGC AGAACTGCTG
    CCCAAGGCCA TGGTAGAGGT CGAAGACTTT GTGGATCCCA ATGGTAAAAT CTATCTGCAG
    CGTGAGGAGA CACCATCTAA CCGTGGTGAA TCTACAGTGC TGAGTTTTGT TAGTTGGCTC
    ACCCTCAGTG GGACGGAGCA ATCTGGTATG AGAGGGCCAT CTACTGAGAC ACAGGAAGCA
    AAGCGAGCAG CTCTGGAGTG CATAAAGCAA TGTGATCCTG AGAAGTTGAT CACAGAAAGT
    AAATTCCTCC AACTTGAATC TCTCCAGGAG CTGATGAAGG CTCTAATCTC TGTGACTCCT
    GATGAGGAGA CATACGATGA AGAGGATGCT GCCTTCTGCT TGGAGATGCT TCTAAGGATT
    GTACTAGAAA ATAGGGACCG TGTGACCTGT GTCTGGCAAA CTGTCCGAGA CCACCTCTAT
    CACCTCTGTG TCAATGCAAT GGAGTTCTGC TTCTTAGTGG AGAGGGCAGT GGTGGGGCTG
    CTGAGGCTGG CGATTCGGCT CCTTCGTCGG GAAGAAATCA GTGCACAGGT TCTCCTCTCA
    TTACGTATCC TGCTTATGAT GAAGCCAAAT GTGTTGTCCA GAGTTAGTCA TGAGGTTGCC
    TATGGCCTCC ATGAACTGCT GAAGACCAAT GCTGCCAACA TTCATTCTGG GGATGACTGG
    TACACTCTCT TCACCCTCCT GGAGTGCATT GGGTCTGGGG TAAAGCCACC TGCAGCCCTG
    CAGGTTACAG CAAGGGCAGA TAACGACACA GGTGCTCAGT CAGACAGTGA AGTCTCCTCC
    TCCTATCATC CAAGTGACAT GAGCCTAGAC CGTGGCTACA CGTCTGATTC AGAGGTGTAC
    ACAGACCATG GGAAGCCAGG CAAGATGCAC CGCTCGGTGA CAGATGTGGA TATGATGAAC
    AGTGGCTGGC TAGTGGTGGG GAAAGATGAC ATCGACACCT CTAAACCCAG TGCTGGGCTC
    AACAGGCTGG GGCCATCTCC TCTTGTCAAC CAATACAGCC TGACGGTGGG GCTGGATTTG
    GGCCCACATG ACACAAAGTC TCTCATGAAG TGTGTAGAGT CCTTGTCCTT CATTGTGCGA
    GATGCTGCCC ACGTGACACC TGAGAACTTT GAGCTCTGTG TCAAAACCAT CCGCATATTT
    GTGGAGGCAA GCTTGAATGG AGGCTCTAAG TCCCAGGAGA AAAGGGGGAA GAGCCACAGA
    CATGATAGTA AAACCAGTCG GTTAAAAAAA AAGCCAAAGG AGAGCTCCAC GCGGCGATCA
    CGGGTCTTGA GCCAGCACCA GGCCCACACA CACAGTGATG AGGACGAGGA TGAGAGCATC
    CCTGCCAGCT ACCACACTGT GTCTTTACAG GTTAGTCAGG ACCTCCTGGA TCTCATGCAC
    ACCTTGCACA CACGAGCAGC TTCCATCTAC AGCTCCTGGG CAGAGGAGCA ACGCCATCTA
    GAGACTTCAG GGAGGAAAAT TGAAGCAGAT TCCCGAACAC TGTGGTCGAA CTGCTGGTGT
    CCTTTGCTAC AAGGTATTGC CTGGCTATGC TGTGATGCCC GACGCCAGGT ACGGATGCAG
    GCACTCACCT ACTTGCAGCG GGCCCTCCTC GTACATGACC TGCAGGCCCT AGATGCCCTA
    GAGTGGGAGT CATGCTTCAA TAAGGTGCTG TTCCCTCTGC TAACGAAGCT CCTTGAGAAC
    ATCAGCCCAG CTGACATTGG TGGAATGGAA GAAACTAGGA TGAGAGCTTC AACGCTGCTC
    TCCAAGGTGT TCCTACAGCA TCTCTCCCCC CTGCTCTCAC TGACCACTTT CGCTGCATTG
    TGGCTGACAA TCCTTGACTT CATGGACAAA TACATGCATG CTGGCTCCAG TGACTTACTG
    CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT ATGCTGCTTG TAATGGACAC AGCTGGGATA
    TTCCACAGTG CAGACTCACG GACAGGATAC TCTGATCTTT GGGAGATCAC CTGGGAGCGG
    ATTGACTGCT TCTTACCTAG GCTGCGAGAT GAGCTCTTCA AACAGACAGT CATCCAAGAT
    CCTGTTCCCA GCATACCAAT GGAACAGCAT CCTCAGAAGT CAATAGCATC TGCCCTGCCC
    CCTTCTCCTG TGGGGGATGT GAGACCAGCC ACCCACCCAG CTCCATCGGA GAAGCCCTCT
    GAACTGAGTG CTAGTGAGTC TGAGAGAGCT TCTGCACCTG CATCCCCCAC CATCAGCAGC
    TCTTCTTCTC CTTCTTTCAC AGCATCAGCG CCAACAAAGA CAAGCCCCGT TACAGACATA
    CCTCCTACAA CAGCACAGCC ACCACTTATC CTGCAGCCTC TTGCATCTCC CCTGCAGGTT
    GGGGTGCCAC CTATGACTTT GCCAATCATC CTCAACCCAG CTCTAATAGA AGCCACCTCT
    CCTGTTCCCC TCTTAGCTAC TCCACGGCCC ACTGACCCCA TGACAACCTC TGAAGTCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143946.1
    CDS236..5938
    Translation

    Target ORF information:

    RefSeq Version XM_015288460.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG TTGTCAAGAT GGTGGATAAA AACATTTACA TTGTACAAGG CGAAATCAAT 
    GTAGTGGTGG GAGCCATCAA GCGTAATGCC CGGTGGAGCA CTCACACACA CCTGGATGAA
    GAAAGGGATC CCTTGCTGCA TAGCTTTAGT GTCTTGAAAG AAGTGCTGAA TAATATAACA
    GAGCTATCTG AAATTGAACC CAACGTCTTT CTTCGACCTT TCCTCGAAGT GATCCGTTCT
    GAAGACACTA CAGGTCCTAT AACTGGGTTG GCCCTCACCT CTGTGAATAA GTTCCTCTCA
    TATGCACTGA TAGATCCCAG CCATGAAGGC ACAGCTGAGG GAATGGAGAA CATGGCAGAT
    GCTGTCACTC ATGCCCGATT TGTGGGTACA GATCATGCGA ATGATGAAGT TGTCTTGATG
    AAAATACTAC AGGTTTTGAG AACCTTGCTG CTCACTCCTG TGGGAGCCCA CCTCACCAAT
    GAATCCGTGT GTGAGATCAT GCAGTCTTGT TTCCGCATAT GCTTTGAAAT GAGACTGAGT
    GAGTTATTGA GAAAATCTGC AGAGCACACT CTGGTCGACA TGGTGCAGCT GCTCTTCACA
    AGGTTGCCTC AGTTCAAAGA AGAGCCTAAA AGCTACATGG GAACTAACAT GAAGAAGATC
    TCTTCATGTC TCCTCAACAA ACTGGAACTA AGTAGTGGGG AACAGCCCAA AGCCCTGAAC
    CAATTAGAGA GGGTATTGCT CTTTAAGAAC CTGAAGTTGA AAATGAGAGC TGGAGGAATG
    AGTGAGTCAT CAAAATGGAA AAAACAGAAG AGATCACCAA GACCTCCTCG ACATGTAACT
    AAGGTCTCCC CTGCAACAGA ACAACCAGCA GCCAATGCTA GTAATGTCAC AGCAAGCGGA
    GTTACTTTCA TAGATGCACC TTCTCCCAGT TCCTCTGGAA GCTCCGAAAA TATTTCATCT
    GCAGTCAGCC CTGCTACAGA CAGTGGTTTG GAAATGTCCT CACAGGCCAC CTCCAAGGAA
    GACCTGACAG ACTTGGACCC GGTCACTTCT TTGGGGCTTC ATGCAGGAAC ACCTTCAAAT
    GAGGCCAAAG TCACTGATAA TCAAAACCAG CCTGAACTCC AGAATGAAAG TCTGAGGGAG
    GAGAAGATCC AGTCAGCCTC TGTTGAATCT ATCCCTGAGG TTTTAGAGGA GTGCACTTCT
    GTAGCAGAAC ATTCTGACTC TGCCTCAGTT CATGACATGG ACTATGTAAA TCCCCGAGGA
    GTACGTTTTA CTCAGTCTTC CCAAAAAGAA GGGGCAGCAC TGGTCCCGTA TGGATTGCCG
    TGTATTAGAG AACTGTTTCG CTTTCTTATC TCGCTTACCA ACCCTCTTGA TCGTCACAAC
    TCTGAGGTGA TGATCCACAT GGGATTGCAG CTGCTGACTG TAGCTCTGGA GTCTGCTCCC
    ATTGCAAACT GCCAGTCTCT CCTAGGACTT GTGAAGGAAG AGTTGTGCCG ACATCTGTTT
    CAACTACTGA GTGTTGAACG GCTCAATCTA TATGCAGCAT CCCTCAGGGT GTGTTTTCTC
    CTCTTTGAAA GTATGAGAGA GCATCTGAAA TTCCAGCTGG AGATGTATAT CAAGAAGTTG
    ATGGAGATAA TCACTGTGGA AAACCCAAAG ATGCCCTATG AAATGAAGGA GATGGCTTTG
    GAAGCCATTG TTCAGCTGTG GAGGATTCCC AGCTTTGTGA CTGAGCTCTA CATCAACTAT
    GACTGTGACT ACTACTGTGC CAACCTCTTT GAGGAGCTCA CTAAGCTGCT CTCCAAGAAT
    GCCTTTCCAG TTTCTGGACA GTTGTATACA GTGCACCTGC TGTCTATGGA GGCATTGCTA
    ACAGTGATAG ATAGCACAGA GGCACATTGC CAGGCCAAAG TGCTGAGTAA CGTACATCAA
    CAAGAGAAGG AAGTTGCCAA ATCAGGCCCA GAAACAATGA ACAGCACCAA AGAAATGAGC
    AATAATAATG AGAGAGCACA CAGTGAGGGA AAATCCACCG CTGTAGTCTC AGAGCCAGGT
    GGGGCATGTC CTCCCACCAG TGGTTGCCTT ATGGCTGATC AGATGAAGCA AGGCTGCATG
    GAATTGGAAG GAGGTGGTGA GACAGCTGAG AAGAATATCC CCAAGAAGCC TACTCGATTT
    TCTTGCATCC TTCCAAGCCC TCAGGAGCTT ATGCACATTA AGAACAAAAA GAAGCTCCTG
    ATAACTGGCA CAGAGCAGTT CAACCAAAAG CCAAAGAAAG GGATACAGTT CCTGCAGGAG
    AAGAACCTTC TTGCCACTCC CATCAATAAT AATGAGGTGG CCAGATGGCT ACGGGAAAAT
    CCTCGCCTGG ACAAGAAGAT GATTGGGGAG TTTGTGAGTG ATCGTAAGAA CATAGATTTA
    CTGGAAAGCT TTGTTGGGAC TTTCAGCTTC CAAGGTTTAA GACTGGATGA AGCTTTACGA
    CTTTATTTAG AGGCCTTCCG ATTACCAGGA GAGGCACCTG TAATCCAGAG ACTGTTGGAA
    GCCTTCACAG AACATTGGAG GAAATCAAAT GGATCCCCAT TTGCTAATAG TGATGCCTGC
    TTTGCCCTGG CCTATGCAGT TATTATGCTG AACACTGACC AGCACAACCA TAATGTCCGC
    AAGCAGAATG TCCCAATGAC TCTGGAGGAG TTTCGGAAAA ACCTGAAAGG TGTGAATGGA
    GGCAAGGACT TCGAACAAGA CATGCTGGAA GACATGTACC ATGCCATCAA AAATGATGAG
    ATAGTGATGC CAGAGGAACA GACAGGCCTG GTGAAAGAAA ACTATATCTG GAATGTCCTG
    CTGCATCGTG GTGCCACTGA TGAGGGAATA TTTCTCCATG TGCCCCCTGG AAGCTACGAC
    CATGATCTCT TCACCATGAC ATGGGGGCCA ACCATTGCAG CACTTTCTTA TGTTTTTGAC
    AAGAGCTTAG AAGAAACAAT CATCCAGAAA GCTATCTCTG GTTTCAGGAA ATGTGCAATG
    ATTTCTGCTC ACTATGGCCT CAGTGATGTG TTTGACAATC TCATAATTTC TCTCTGCAAG
    TTTACAGCCC TCAGCAGCGA GTCTATTGAG AACCTGCCGA CTGTTTTTGG AAGTAATCCC
    AAGGCCCATA TTGCAGCAAA GACTGTGTTT CACTTGGCCC ATCGCCACGG TGACATTCTG
    CGGGAGGGCT GGAAAAACAT CATGGAGGCC CTCCTGCAGC TTTTCCGAGC AGAACTGCTG
    CCCAAGGCCA TGGTAGAGGT CGAAGACTTT GTGGATCCCA ATGGTAAAAT CTATCTGCAG
    CGTGAGGAGA CACCATCTAA CCGTGGTGAA TCTACAGTGC TGAGTTTTGT TAGTTGGCTC
    ACCCTCAGTG GGACGGAGCA ATCTGGTATG AGAGGGCCAT CTACTGAGAC ACAGGAAGCA
    AAGCGAGCAG CTCTGGAGTG CATAAAGCAA TGTGATCCTG AGAAGTTGAT CACAGAAAGT
    AAATTCCTCC AACTTGAATC TCTCCAGGAG CTGATGAAGG CTCTAATCTC TGTGACTCCT
    GATGAGGAGA CATACGATGA AGAGGATGCT GCCTTCTGCT TGGAGATGCT TCTAAGGATT
    GTACTAGAAA ATAGGGACCG TGTGACCTGT GTCTGGCAAA CTGTCCGAGA CCACCTCTAT
    CACCTCTGTG TCAATGCAAT GGAGTTCTGC TTCTTAGTGG AGAGGGCAGT GGTGGGGCTG
    CTGAGGCTGG CGATTCGGCT CCTTCGTCGG GAAGAAATCA GTGCACAGGT TCTCCTCTCA
    TTACGTATCC TGCTTATGAT GAAGCCAAAT GTGTTGTCCA GAGTTAGTCA TGAGGTTGCC
    TATGGCCTCC ATGAACTGCT GAAGACCAAT GCTGCCAACA TTCATTCTGG GGATGACTGG
    TACACTCTCT TCACCCTCCT GGAGTGCATT GGGTCTGGGG TAAAGCCACC TGCAGCCCTG
    CAGGTTACAG CAAGGGCAGA TAACGACACA GGTGCTCAGT CAGACAGTGA AGTCTCCTCC
    TCCTATCATC CAAGTGACAT GAGCCTAGAC CGTGGCTACA CGTCTGATTC AGAGGTGTAC
    ACAGACCATG GGAAGCCAGG CAAGATGCAC CGCTCGGTGA CAGATGTGGA TATGATGAAC
    AGTGGCTGGC TAGTGGTGGG GAAAGATGAC ATCGACACCT CTAAACCCAG TGCTGGGCTC
    AACAGGCTGG GGCCATCTCC TCTTGTCAAC CAATACAGCC TGACGGTGGG GCTGGATTTG
    GGCCCACATG ACACAAAGTC TCTCATGAAG TGTGTAGAGT CCTTGTCCTT CATTGTGCGA
    GATGCTGCCC ACGTGACACC TGAGAACTTT GAGCTCTGTG TCAAAACCAT CCGCATATTT
    GTGGAGGCAA GCTTGAATGG AGGCTCTAAG TCCCAGGAGA AAAGGGGGAA GAGCCACAGA
    CATGATAGTA AAACCAGTCG GTTAAAAAAA AAGCCAAAGG AGAGCTCCAC GCGGCGATCA
    CGGGTCTTGA GCCAGCACCA GGCCCACACA CACAGTGATG AGGACGAGGA TGAGAGCATC
    CCTGCCAGCT ACCACACTGT GTCTTTACAG GTTAGTCAGG ACCTCCTGGA TCTCATGCAC
    ACCTTGCACA CACGAGCAGC TTCCATCTAC AGCTCCTGGG CAGAGGAGCA ACGCCATCTA
    GAGACTTCAG GGAGGAAAAT TGAAGCAGAT TCCCGAACAC TGTGGTCGAA CTGCTGGTGT
    CCTTTGCTAC AAGGTATTGC CTGGCTATGC TGTGATGCCC GACGCCAGGT ACGGATGCAG
    GCACTCACCT ACTTGCAGCG GGCCCTCCTC GTACATGACC TGCAGGCCCT AGATGCCCTA
    GAGTGGGAGT CATGCTTCAA TAAGGTGCTG TTCCCTCTGC TAACGAAGCT CCTTGAGAAC
    ATCAGCCCAG CTGACATTGG TGGAATGGAA GAAACTAGGA TGAGAGCTTC AACGCTGCTC
    TCCAAGGTGT TCCTACAGCA TCTCTCCCCC CTGCTCTCAC TGACCACTTT CGCTGCATTG
    TGGCTGACAA TCCTTGACTT CATGGACAAA TACATGCATG CTGGCTCCAG TGACTTACTG
    CTGGAGGCCA TCCCAGAGTC TCTGAAGAAT ATGCTGCTTG TAATGGACAC AGCTGGGATA
    TTCCACAGTG CAGACTCACG GACAGGATAC TCTGATCTTT GGGAGATCAC CTGGGAGCGG
    ATTGACTGCT TCTTACCTAG GCTGCGAGAT GAGCTCTTCA AACAGACAGT CATCCAAGAT
    CCTGTTCCCA GCATACCAAT GGAACAGCAT CCTCAGAAGT CAATAGCATC TGCCCTGCCC
    CCTTCTCCTG TGGGGGATGT GAGACCAGCC ACCCACCCAG CTCCATCGGA GAAGCCCTCT
    GAACTGAGTG CTAGTGAGTC TGAGAGAGCT TCTGCACCTG CATCCCCCAC CATCAGCAGC
    TCTTCTTCTC CTTCTTTCAC AGCATCAGCG CCAACAAAGA CAAGCCCCGT TACAGACATA
    CCTCCTACAA CAGCACAGCC ACCACTTATC CTGCAGCCTC TTGCATCTCC CCTGCAGGTT
    GGGGTGCCAC CTATGACTTT GCCAATCATC CTCAACCCAG CTCTAATAGA AGCCACCTCT
    CCTGTTCCCC TCTTAGCTAC TCCACGGCCC ACTGACCCCA TGACAACCTC TGAAGTCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37630
    Clone ID Related Accession (Same CDS sequence) XM_015288461.1 , XM_015288461.2
    Accession Version XM_015288461.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGGTTAGTC AGGACCTCCT GGATCTCATG
    CACACCTTGC ACACACGAGC AGCTTCCATC TACAGCTCCT GGGCAGAGGA GCAACGCCAT
    CTAGAGACTT CAGGGAGGAA AATTGAAGCA GATTCCCGAA CACTGTGGTC GAACTGCTGG
    TGTCCTTTGC TACAAGGTAT TGCCTGGCTA TGCTGTGATG CCCGACGCCA GGTACGGATG
    CAGGCACTCA CCTACTTGCA GCGGGCCCTC CTCGTACATG ACCTGCAGGC CCTAGATGCC
    CTAGAGTGGG AGTCATGCTT CAATAAGGTG CTGTTCCCTC TGCTAACGAA GCTCCTTGAG
    AACATCAGCC CAGCTGACAT TGGTGGAATG GAAGAAACTA GGATGAGAGC TTCAACGCTG
    CTCTCCAAGG TGTTCCTACA GCATCTCTCC CCCCTGCTCT CACTGACCAC TTTCGCTGCA
    TTGTGGCTGA CAATCCTTGA CTTCATGGAC AAATACATGC ATGCTGGCTC CAGTGACTTA
    CTGCTGGAGG CCATCCCAGA GTCTCTGAAG AATATGCTGC TTGTAATGGA CACAGCTGGG
    ATATTCCACA GTGCAGACTC ACGGACAGGA TACTCTGATC TTTGGGAGAT CACCTGGGAG
    CGGATTGACT GCTTCTTACC TAGGCTGCGA GATGAGCTCT TCAAACAGAC AGTCATCCAA
    GATCCTGTTC CCAGCATACC AATGGAACAG CATCCTCAGA AGTCAATAGC ATCTGCCCTG
    CCCCCTTCTC CTGTGGGGGA TGTGAGACCA GCCACCCACC CAGCTCCATC GGAGAAGCCC
    TCTGAACTGA GTGCTAGTGA GTCTGAGAGA GCTTCTGCAC CTGCATCCCC CACCATCAGC
    AGCTCTTCTT CTCCTTCTTT CACAGCATCA GCGCCAACAA AGACAAGCCC CGTTACAGAC
    ATACCTCCTA CAACAGCACA GCCACCACTT ATCCTGCAGC CTCTTGCATC TCCCCTGCAG
    GTTGGGGTGC CACCTATGAC TTTGCCAATC ATCCTCAACC CAGCTCTAAT AGAAGCCACC
    TCTCCTGTTC CCCTCTTAGC TACTCCACGG CCCACTGACC CCATGACAAC CTCTGAAGTC
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143947.1
    CDS11..5596
    Translation

    Target ORF information:

    RefSeq Version XM_015288461.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGGTTAGTC AGGACCTCCT GGATCTCATG
    CACACCTTGC ACACACGAGC AGCTTCCATC TACAGCTCCT GGGCAGAGGA GCAACGCCAT
    CTAGAGACTT CAGGGAGGAA AATTGAAGCA GATTCCCGAA CACTGTGGTC GAACTGCTGG
    TGTCCTTTGC TACAAGGTAT TGCCTGGCTA TGCTGTGATG CCCGACGCCA GGTACGGATG
    CAGGCACTCA CCTACTTGCA GCGGGCCCTC CTCGTACATG ACCTGCAGGC CCTAGATGCC
    CTAGAGTGGG AGTCATGCTT CAATAAGGTG CTGTTCCCTC TGCTAACGAA GCTCCTTGAG
    AACATCAGCC CAGCTGACAT TGGTGGAATG GAAGAAACTA GGATGAGAGC TTCAACGCTG
    CTCTCCAAGG TGTTCCTACA GCATCTCTCC CCCCTGCTCT CACTGACCAC TTTCGCTGCA
    TTGTGGCTGA CAATCCTTGA CTTCATGGAC AAATACATGC ATGCTGGCTC CAGTGACTTA
    CTGCTGGAGG CCATCCCAGA GTCTCTGAAG AATATGCTGC TTGTAATGGA CACAGCTGGG
    ATATTCCACA GTGCAGACTC ACGGACAGGA TACTCTGATC TTTGGGAGAT CACCTGGGAG
    CGGATTGACT GCTTCTTACC TAGGCTGCGA GATGAGCTCT TCAAACAGAC AGTCATCCAA
    GATCCTGTTC CCAGCATACC AATGGAACAG CATCCTCAGA AGTCAATAGC ATCTGCCCTG
    CCCCCTTCTC CTGTGGGGGA TGTGAGACCA GCCACCCACC CAGCTCCATC GGAGAAGCCC
    TCTGAACTGA GTGCTAGTGA GTCTGAGAGA GCTTCTGCAC CTGCATCCCC CACCATCAGC
    AGCTCTTCTT CTCCTTCTTT CACAGCATCA GCGCCAACAA AGACAAGCCC CGTTACAGAC
    ATACCTCCTA CAACAGCACA GCCACCACTT ATCCTGCAGC CTCTTGCATC TCCCCTGCAG
    GTTGGGGTGC CACCTATGAC TTTGCCAATC ATCCTCAACC CAGCTCTAAT AGAAGCCACC
    TCTCCTGTTC CCCTCTTAGC TACTCCACGG CCCACTGACC CCATGACAAC CTCTGAAGTC
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37630
    Clone ID Related Accession (Same CDS sequence) XM_015288461.1 , XM_015288461.2
    Accession Version XM_015288461.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGGTTAGTC AGGACCTCCT GGATCTCATG
    CACACCTTGC ACACACGAGC AGCTTCCATC TACAGCTCCT GGGCAGAGGA GCAACGCCAT
    CTAGAGACTT CAGGGAGGAA AATTGAAGCA GATTCCCGAA CACTGTGGTC GAACTGCTGG
    TGTCCTTTGC TACAAGGTAT TGCCTGGCTA TGCTGTGATG CCCGACGCCA GGTACGGATG
    CAGGCACTCA CCTACTTGCA GCGGGCCCTC CTCGTACATG ACCTGCAGGC CCTAGATGCC
    CTAGAGTGGG AGTCATGCTT CAATAAGGTG CTGTTCCCTC TGCTAACGAA GCTCCTTGAG
    AACATCAGCC CAGCTGACAT TGGTGGAATG GAAGAAACTA GGATGAGAGC TTCAACGCTG
    CTCTCCAAGG TGTTCCTACA GCATCTCTCC CCCCTGCTCT CACTGACCAC TTTCGCTGCA
    TTGTGGCTGA CAATCCTTGA CTTCATGGAC AAATACATGC ATGCTGGCTC CAGTGACTTA
    CTGCTGGAGG CCATCCCAGA GTCTCTGAAG AATATGCTGC TTGTAATGGA CACAGCTGGG
    ATATTCCACA GTGCAGACTC ACGGACAGGA TACTCTGATC TTTGGGAGAT CACCTGGGAG
    CGGATTGACT GCTTCTTACC TAGGCTGCGA GATGAGCTCT TCAAACAGAC AGTCATCCAA
    GATCCTGTTC CCAGCATACC AATGGAACAG CATCCTCAGA AGTCAATAGC ATCTGCCCTG
    CCCCCTTCTC CTGTGGGGGA TGTGAGACCA GCCACCCACC CAGCTCCATC GGAGAAGCCC
    TCTGAACTGA GTGCTAGTGA GTCTGAGAGA GCTTCTGCAC CTGCATCCCC CACCATCAGC
    AGCTCTTCTT CTCCTTCTTT CACAGCATCA GCGCCAACAA AGACAAGCCC CGTTACAGAC
    ATACCTCCTA CAACAGCACA GCCACCACTT ATCCTGCAGC CTCTTGCATC TCCCCTGCAG
    GTTGGGGTGC CACCTATGAC TTTGCCAATC ATCCTCAACC CAGCTCTAAT AGAAGCCACC
    TCTCCTGTTC CCCTCTTAGC TACTCCACGG CCCACTGACC CCATGACAAC CTCTGAAGTC
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143947.1
    CDS11..5596
    Translation

    Target ORF information:

    RefSeq Version XM_015288461.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGGTTAGTC AGGACCTCCT GGATCTCATG
    CACACCTTGC ACACACGAGC AGCTTCCATC TACAGCTCCT GGGCAGAGGA GCAACGCCAT
    CTAGAGACTT CAGGGAGGAA AATTGAAGCA GATTCCCGAA CACTGTGGTC GAACTGCTGG
    TGTCCTTTGC TACAAGGTAT TGCCTGGCTA TGCTGTGATG CCCGACGCCA GGTACGGATG
    CAGGCACTCA CCTACTTGCA GCGGGCCCTC CTCGTACATG ACCTGCAGGC CCTAGATGCC
    CTAGAGTGGG AGTCATGCTT CAATAAGGTG CTGTTCCCTC TGCTAACGAA GCTCCTTGAG
    AACATCAGCC CAGCTGACAT TGGTGGAATG GAAGAAACTA GGATGAGAGC TTCAACGCTG
    CTCTCCAAGG TGTTCCTACA GCATCTCTCC CCCCTGCTCT CACTGACCAC TTTCGCTGCA
    TTGTGGCTGA CAATCCTTGA CTTCATGGAC AAATACATGC ATGCTGGCTC CAGTGACTTA
    CTGCTGGAGG CCATCCCAGA GTCTCTGAAG AATATGCTGC TTGTAATGGA CACAGCTGGG
    ATATTCCACA GTGCAGACTC ACGGACAGGA TACTCTGATC TTTGGGAGAT CACCTGGGAG
    CGGATTGACT GCTTCTTACC TAGGCTGCGA GATGAGCTCT TCAAACAGAC AGTCATCCAA
    GATCCTGTTC CCAGCATACC AATGGAACAG CATCCTCAGA AGTCAATAGC ATCTGCCCTG
    CCCCCTTCTC CTGTGGGGGA TGTGAGACCA GCCACCCACC CAGCTCCATC GGAGAAGCCC
    TCTGAACTGA GTGCTAGTGA GTCTGAGAGA GCTTCTGCAC CTGCATCCCC CACCATCAGC
    AGCTCTTCTT CTCCTTCTTT CACAGCATCA GCGCCAACAA AGACAAGCCC CGTTACAGAC
    ATACCTCCTA CAACAGCACA GCCACCACTT ATCCTGCAGC CTCTTGCATC TCCCCTGCAG
    GTTGGGGTGC CACCTATGAC TTTGCCAATC ATCCTCAACC CAGCTCTAAT AGAAGCCACC
    TCTCCTGTTC CCCTCTTAGC TACTCCACGG CCCACTGACC CCATGACAAC CTCTGAAGTC
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37631
    Clone ID Related Accession (Same CDS sequence) XM_015288462.1
    Accession Version XM_015288462.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGCTCCTGG ATCTCATGCA CACCTTGCAC
    ACACGAGCAG CTTCCATCTA CAGCTCCTGG GCAGAGGAGC AACGCCATCT AGAGACTTCA
    GGGAGGAAAA TTGAAGCAGA TTCCCGAACA CTGTGGTCGA ACTGCTGGTG TCCTTTGCTA
    CAAGGTATTG CCTGGCTATG CTGTGATGCC CGACGCCAGG TACGGATGCA GGCACTCACC
    TACTTGCAGC GGGCCCTCCT CGTACATGAC CTGCAGGCCC TAGATGCCCT AGAGTGGGAG
    TCATGCTTCA ATAAGGTGCT GTTCCCTCTG CTAACGAAGC TCCTTGAGAA CATCAGCCCA
    GCTGACATTG GTGGAATGGA AGAAACTAGG ATGAGAGCTT CAACGCTGCT CTCCAAGGTG
    TTCCTACAGC ATCTCTCCCC CCTGCTCTCA CTGACCACTT TCGCTGCATT GTGGCTGACA
    ATCCTTGACT TCATGGACAA ATACATGCAT GCTGGCTCCA GTGACTTACT GCTGGAGGCC
    ATCCCAGAGT CTCTGAAGAA TATGCTGCTT GTAATGGACA CAGCTGGGAT ATTCCACAGT
    GCAGACTCAC GGACAGGATA CTCTGATCTT TGGGAGATCA CCTGGGAGCG GATTGACTGC
    TTCTTACCTA GGCTGCGAGA TGAGCTCTTC AAACAGACAG TCATCCAAGA TCCTGTTCCC
    AGCATACCAA TGGAACAGCA TCCTCAGAAG TCAATAGCAT CTGCCCTGCC CCCTTCTCCT
    GTGGGGGATG TGAGACCAGC CACCCACCCA GCTCCATCGG AGAAGCCCTC TGAACTGAGT
    GCTAGTGAGT CTGAGAGAGC TTCTGCACCT GCATCCCCCA CCATCAGCAG CTCTTCTTCT
    CCTTCTTTCA CAGCATCAGC GCCAACAAAG ACAAGCCCCG TTACAGACAT ACCTCCTACA
    ACAGCACAGC CACCACTTAT CCTGCAGCCT CTTGCATCTC CCCTGCAGGT TGGGGTGCCA
    CCTATGACTT TGCCAATCAT CCTCAACCCA GCTCTAATAG AAGCCACCTC TCCTGTTCCC
    CTCTTAGCTA CTCCACGGCC CACTGACCCC ATGACAACCT CTGAAGTCAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143948.1
    CDS11..5584
    Translation

    Target ORF information:

    RefSeq Version XM_015288462.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAACATTTA CATTGTACAA GGCGAAATCA ATGTAGTGGT GGGAGCCATC 
    AAGCGTAATG CCCGGTGGAG CACTCACACA CACCTGGATG AAGAAAGGGA TCCCTTGCTG
    CATAGCTTTA GTGTCTTGAA AGAAGTGCTG AATAATATAA CAGAGCTATC TGAAATTGAA
    CCCAACGTCT TTCTTCGACC TTTCCTCGAA GTGATCCGTT CTGAAGACAC TACAGGTCCT
    ATAACTGGGT TGGCCCTCAC CTCTGTGAAT AAGTTCCTCT CATATGCACT GATAGATCCC
    AGCCATGAAG GCACAGCTGA GGGAATGGAG AACATGGCAG ATGCTGTCAC TCATGCCCGA
    TTTGTGGGTA CAGATCATGC GAATGATGAA GTTGTCTTGA TGAAAATACT ACAGGTTTTG
    AGAACCTTGC TGCTCACTCC TGTGGGAGCC CACCTCACCA ATGAATCCGT GTGTGAGATC
    ATGCAGTCTT GTTTCCGCAT ATGCTTTGAA ATGAGACTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTGGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTGCC TCAGTTCAAA
    GAAGAGCCTA AAAGCTACAT GGGAACTAAC ATGAAGAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGAGT CATCAAAATG GAAAAAACAG AAGAGATCAC CAAGACCTCC TCGACATGTA
    ACTAAGGTCT CCCCTGCAAC AGAACAACCA GCAGCCAATG CTAGTAATGT CACAGCAAGC
    GGAGTTACTT TCATAGATGC ACCTTCTCCC AGTTCCTCTG GAAGCTCCGA AAATATTTCA
    TCTGCAGTCA GCCCTGCTAC AGACAGTGGT TTGGAAATGT CCTCACAGGC CACCTCCAAG
    GAAGACCTGA CAGACTTGGA CCCGGTCACT TCTTTGGGGC TTCATGCAGG AACACCTTCA
    AATGAGGCCA AAGTCACTGA TAATCAAAAC CAGCCTGAAC TCCAGAATGA AAGTCTGAGG
    GAGGAGAAGA TCCAGTCAGC CTCTGTTGAA TCTATCCCTG AGGTTTTAGA GGAGTGCACT
    TCTGTAGCAG AACATTCTGA CTCTGCCTCA GTTCATGACA TGGACTATGT AAATCCCCGA
    GGAGTACGTT TTACTCAGTC TTCCCAAAAA GAAGGGGCAG CACTGGTCCC GTATGGATTG
    CCGTGTATTA GAGAACTGTT TCGCTTTCTT ATCTCGCTTA CCAACCCTCT TGATCGTCAC
    AACTCTGAGG TGATGATCCA CATGGGATTG CAGCTGCTGA CTGTAGCTCT GGAGTCTGCT
    CCCATTGCAA ACTGCCAGTC TCTCCTAGGA CTTGTGAAGG AAGAGTTGTG CCGACATCTG
    TTTCAACTAC TGAGTGTTGA ACGGCTCAAT CTATATGCAG CATCCCTCAG GGTGTGTTTT
    CTCCTCTTTG AAAGTATGAG AGAGCATCTG AAATTCCAGC TGGAGATGTA TATCAAGAAG
    TTGATGGAGA TAATCACTGT GGAAAACCCA AAGATGCCCT ATGAAATGAA GGAGATGGCT
    TTGGAAGCCA TTGTTCAGCT GTGGAGGATT CCCAGCTTTG TGACTGAGCT CTACATCAAC
    TATGACTGTG ACTACTACTG TGCCAACCTC TTTGAGGAGC TCACTAAGCT GCTCTCCAAG
    AATGCCTTTC CAGTTTCTGG ACAGTTGTAT ACAGTGCACC TGCTGTCTAT GGAGGCATTG
    CTAACAGTGA TAGATAGCAC AGAGGCACAT TGCCAGGCCA AAGTGCTGAG TAACGTACAT
    CAACAAGAGA AGGAAGTTGC CAAATCAGGC CCAGAAACAA TGAACAGCAC CAAAGAAATG
    AGCAATAATA ATGAGAGAGC ACACAGTGAG GGAAAATCCA CCGCTGTAGT CTCAGAGCCA
    GGTGGGGCAT GTCCTCCCAC CAGTGGTTGC CTTATGGCTG ATCAGATGAA GCAAGGCTGC
    ATGGAATTGG AAGGAGGTGG TGAGACAGCT GAGAAGAATA TCCCCAAGAA GCCTACTCGA
    TTTTCTTGCA TCCTTCCAAG CCCTCAGGAG CTTATGCACA TTAAGAACAA AAAGAAGCTC
    CTGATAACTG GCACAGAGCA GTTCAACCAA AAGCCAAAGA AAGGGATACA GTTCCTGCAG
    GAGAAGAACC TTCTTGCCAC TCCCATCAAT AATAATGAGG TGGCCAGATG GCTACGGGAA
    AATCCTCGCC TGGACAAGAA GATGATTGGG GAGTTTGTGA GTGATCGTAA GAACATAGAT
    TTACTGGAAA GCTTTGTTGG GACTTTCAGC TTCCAAGGTT TAAGACTGGA TGAAGCTTTA
    CGACTTTATT TAGAGGCCTT CCGATTACCA GGAGAGGCAC CTGTAATCCA GAGACTGTTG
    GAAGCCTTCA CAGAACATTG GAGGAAATCA AATGGATCCC CATTTGCTAA TAGTGATGCC
    TGCTTTGCCC TGGCCTATGC AGTTATTATG CTGAACACTG ACCAGCACAA CCATAATGTC
    CGCAAGCAGA ATGTCCCAAT GACTCTGGAG GAGTTTCGGA AAAACCTGAA AGGTGTGAAT
    GGAGGCAAGG ACTTCGAACA AGACATGCTG GAAGACATGT ACCATGCCAT CAAAAATGAT
    GAGATAGTGA TGCCAGAGGA ACAGACAGGC CTGGTGAAAG AAAACTATAT CTGGAATGTC
    CTGCTGCATC GTGGTGCCAC TGATGAGGGA ATATTTCTCC ATGTGCCCCC TGGAAGCTAC
    GACCATGATC TCTTCACCAT GACATGGGGG CCAACCATTG CAGCACTTTC TTATGTTTTT
    GACAAGAGCT TAGAAGAAAC AATCATCCAG AAAGCTATCT CTGGTTTCAG GAAATGTGCA
    ATGATTTCTG CTCACTATGG CCTCAGTGAT GTGTTTGACA ATCTCATAAT TTCTCTCTGC
    AAGTTTACAG CCCTCAGCAG CGAGTCTATT GAGAACCTGC CGACTGTTTT TGGAAGTAAT
    CCCAAGGCCC ATATTGCAGC AAAGACTGTG TTTCACTTGG CCCATCGCCA CGGTGACATT
    CTGCGGGAGG GCTGGAAAAA CATCATGGAG GCCCTCCTGC AGCTTTTCCG AGCAGAACTG
    CTGCCCAAGG CCATGGTAGA GGTCGAAGAC TTTGTGGATC CCAATGGTAA AATCTATCTG
    CAGCGTGAGG AGACACCATC TAACCGTGGT GAATCTACAG TGCTGAGTTT TGTTAGTTGG
    CTCACCCTCA GTGGGACGGA GCAATCTGGT ATGAGAGGGC CATCTACTGA GACACAGGAA
    GCAAAGCGAG CAGCTCTGGA GTGCATAAAG CAATGTGATC CTGAGAAGTT GATCACAGAA
    AGTAAATTCC TCCAACTTGA ATCTCTCCAG GAGCTGATGA AGGCTCTAAT CTCTGTGACT
    CCTGATGAGG AGACATACGA TGAAGAGGAT GCTGCCTTCT GCTTGGAGAT GCTTCTAAGG
    ATTGTACTAG AAAATAGGGA CCGTGTGACC TGTGTCTGGC AAACTGTCCG AGACCACCTC
    TATCACCTCT GTGTCAATGC AATGGAGTTC TGCTTCTTAG TGGAGAGGGC AGTGGTGGGG
    CTGCTGAGGC TGGCGATTCG GCTCCTTCGT CGGGAAGAAA TCAGTGCACA GGTTCTCCTC
    TCATTACGTA TCCTGCTTAT GATGAAGCCA AATGTGTTGT CCAGAGTTAG TCATGAGGTT
    GCCTATGGCC TCCATGAACT GCTGAAGACC AATGCTGCCA ACATTCATTC TGGGGATGAC
    TGGTACACTC TCTTCACCCT CCTGGAGTGC ATTGGGTCTG GGGTAAAGCC ACCTGCAGCC
    CTGCAGGTTA CAGCAAGGGC AGATAACGAC ACAGGTGCTC AGTCAGACAG TGAAGTCTCC
    TCCTCCTATC ATCCAAGTGA CATGAGCCTA GACCGTGGCT ACACGTCTGA TTCAGAGGTG
    TACACAGACC ATGGGAAGCC AGGCAAGATG CACCGCTCGG TGACAGATGT GGATATGATG
    AACAGTGGCT GGCTAGTGGT GGGGAAAGAT GACATCGACA CCTCTAAACC CAGTGCTGGG
    CTCAACAGGC TGGGGCCATC TCCTCTTGTC AACCAATACA GCCTGACGGT GGGGCTGGAT
    TTGGGCCCAC ATGACACAAA GTCTCTCATG AAGTGTGTAG AGTCCTTGTC CTTCATTGTG
    CGAGATGCTG CCCACGTGAC ACCTGAGAAC TTTGAGCTCT GTGTCAAAAC CATCCGCATA
    TTTGTGGAGG CAAGCTTGAA TGGAGGCTCT AAGTCCCAGG AGAAAAGGGG GAAGAGCCAC
    AGACATGATA GTAAAACCAG TCGGTTAAAA AAAAAGCCAA AGGAGAGCTC CACGCGGCGA
    TCACGGGTCT TGAGCCAGCA CCAGGCCCAC ACACACAGTG ATGAGGACGA GGATGAGAGC
    ATCCCTGCCA GCTACCACAC TGTGTCTTTA CAGCTCCTGG ATCTCATGCA CACCTTGCAC
    ACACGAGCAG CTTCCATCTA CAGCTCCTGG GCAGAGGAGC AACGCCATCT AGAGACTTCA
    GGGAGGAAAA TTGAAGCAGA TTCCCGAACA CTGTGGTCGA ACTGCTGGTG TCCTTTGCTA
    CAAGGTATTG CCTGGCTATG CTGTGATGCC CGACGCCAGG TACGGATGCA GGCACTCACC
    TACTTGCAGC GGGCCCTCCT CGTACATGAC CTGCAGGCCC TAGATGCCCT AGAGTGGGAG
    TCATGCTTCA ATAAGGTGCT GTTCCCTCTG CTAACGAAGC TCCTTGAGAA CATCAGCCCA
    GCTGACATTG GTGGAATGGA AGAAACTAGG ATGAGAGCTT CAACGCTGCT CTCCAAGGTG
    TTCCTACAGC ATCTCTCCCC CCTGCTCTCA CTGACCACTT TCGCTGCATT GTGGCTGACA
    ATCCTTGACT TCATGGACAA ATACATGCAT GCTGGCTCCA GTGACTTACT GCTGGAGGCC
    ATCCCAGAGT CTCTGAAGAA TATGCTGCTT GTAATGGACA CAGCTGGGAT ATTCCACAGT
    GCAGACTCAC GGACAGGATA CTCTGATCTT TGGGAGATCA CCTGGGAGCG GATTGACTGC
    TTCTTACCTA GGCTGCGAGA TGAGCTCTTC AAACAGACAG TCATCCAAGA TCCTGTTCCC
    AGCATACCAA TGGAACAGCA TCCTCAGAAG TCAATAGCAT CTGCCCTGCC CCCTTCTCCT
    GTGGGGGATG TGAGACCAGC CACCCACCCA GCTCCATCGG AGAAGCCCTC TGAACTGAGT
    GCTAGTGAGT CTGAGAGAGC TTCTGCACCT GCATCCCCCA CCATCAGCAG CTCTTCTTCT
    CCTTCTTTCA CAGCATCAGC GCCAACAAAG ACAAGCCCCG TTACAGACAT ACCTCCTACA
    ACAGCACAGC CACCACTTAT CCTGCAGCCT CTTGCATCTC CCCTGCAGGT TGGGGTGCCA
    CCTATGACTT TGCCAATCAT CCTCAACCCA GCTCTAATAG AAGCCACCTC TCCTGTTCCC
    CTCTTAGCTA CTCCACGGCC CACTGACCCC ATGACAACCT CTGAAGTCAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.