Limch1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Limch1
Entrez Gene ID	305332
Full Name	LIM and calponin homology domains 1
Synonyms	RGD1305269
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599190.1	XP_017454679.1	LIM and calponin homology domains-containing protein 1 isoform X4
NM_001191678.1	NP_001178607.1	LIM and calponin homology domains-containing protein 1
XM_017599187.1	XP_017454676.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_017599192.1	XP_017454681.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_017599191.1	XP_017454680.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_017599188.1	XP_017454677.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_017599189.1	XP_017454678.1	LIM and calponin homology domains-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1
Homo sapiens (human)	LIMCH1	NP_055803.2

Gene Ontology
Bibliography

Related articles in PubMed

LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration.
Lin YH, Zhen YY, Chien KY, Lee IC, Lin WC, Chen MY, Pai LM
Molecular biology of the cell28(8)1054-1065(2017 Apr)

Expression and localization of an agmatinase-like protein in the rat brain.
Mella C, Mart?nez F, de Los Angeles Garc?a M, Nualart F, Castro V, Bustos P, Carvajal N, Uribe E
Histochemistry and cell biology134(2)137-44(2010 Aug)

Cloning and functional expression of a rodent brain cDNA encoding a novel protein with agmatinase activity, but not belonging to the arginase family.
Uribe E, Salas M, Enr?quez S, Orellana MS, Carvajal N
Archives of biochemistry and biophysics461(1)146-50(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An agmatinase-like protein was detected in the hypothalamus in glial cells and arcuate nucleus neurons, and in hippocampus astrocytes and neurons, but not in brain cortex
Title: Expression and localization of an agmatinase-like protein in the rat brain.

The following Limch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Limch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa36065	XM_017599192.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa46262	XM_017599191.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa58290	XM_017599187.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58291	XM_017599188.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58292	XM_017599189.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58293	XM_017599190.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa07380	NM_001191678.1 Latest version!	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Limch1 ( XM_017599192.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454681.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa36065
Clone ID Related Accession (Same CDS sequence)	XM_017599192.1
Accession Version	XM_017599192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAAGACAGGA GACTGATGAC ATTGAAAGCC CCAAGCGCAG CATCCGAGAC 
AGTGGCTACA TCGACTGCTG GGACTCTGAA CGCAGTGACT CCCTTTCTCC TCCTCGCCAC 
GGCCGGGATG ACTCCTTTGA CAGCCTGGAT TCTTTTGGCT CCCGCTCCAG GCAGACACCT 
TCACCAGATG TAGTTCTCAG GGGAAGCAGT GATGGAAGAG GAAGTGATTC TGAATCAGAC 
TTGCCCCACC GGAAGTTGCC AGATGTGAAG AAAGATGATA TGTCCGCACG ACGGACTTCT 
CATGGTGAGC CGAAATCAGC AGTGCCTTTT AACCAGTACC TCCCGAACAA AAGCAACCAG 
ACAGCCTATG TCCCAGCCCC TCTGAGAAAG AAGAAGGCGG AGCGGGAAGA ATACAGGAAG 
AGCTGGAGTA CGGCCACCTC CCCCCTGGGC GGGGAAAGGC CCTTCAGATA CGGTCCGAGA 
ACTCCTGTGT CAGATGACGC AGAGAGCACG AGTATGTTTG ACATGCGGTG TGAGGAGGAG 
GCAGCGGTGC TGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GCTGGAAGAG CCGTCGAAGA 
AGCGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTGATG 
TCTGGAGAAG ATGGGACAAG TGAACGGAGG AAAAGCATCA AAACTTACAG AGAAATTGTC 
CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CACGAGGCAT ATAAGAATGC AAGGTCACAG 
GAGGAGGCAG AGGGGATCCT CCAGCAATAC ATCGAGAGGT TCACCATCAG TGAGGCTGTC 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAACGTA 
TCCTCCTTCC CCAACGACCC CAGCCCCATG AAATACTTGC GTCAGCAGTC ACTGCCCCCG 
CCCAAGTTCA CTGCCACGGT TGAGACTACC ATCGCTCGCA CCAGTGTCCC AGAGAGCAGC 
GTAGCAGCAG GCACTGGGTC TCCAAGCAAA ACCAGCACTC CAAACACGGT GCCTATGCTG 
ACACCCAGGC CTTACTCCCA GCCCAAAAAC TCTCAAGAGG TTCTGAAGAC TTTTAAGGTC 
GATGGGAAAG TCAGCATGAA TGGAGAAACG GCCCGTGGAG ATGTGGAAGG AAAGGAAAAA 
GAAGACCCCA CAGCGGTGGC CCCTGGCCCT TCCTTAACCA AGTCCCAGAT GTTTGAAGGC 
GTGGCCACGG TGCATGGCTC TCCCGTGCAA GTGAAACAAG GCAGCAACAG CATCGAGATC 
AACATCAAGA AGCCAAATTC TCCTCCCCAG GAACTGACAG CAGCCTCTGA GGAAACTGAG 
TCAAACGGCC GAGATGATGA GAACGGTGAA GAGAGCTCAG GGGCACGGGA TGTGGAGCTG 
GATTCGGCAG AGCCACAGCA TTTTACAACA ACCGTGACTC GGTGCAGCCC AACCGTGGCC 
CTGGTGGAGT TTTCTTCCAG CCCGCAGCTG AGGAATGAAG TACCAGAAGA ACAAGACCAG 
AAGAAGCCAG AAAACGAGAT GAGCGGGAAG GTGGAGTTAG TACTGTCCCA GAAGGTGGCA 
AAGCCAAAAT CCCCAGAACC GGAAGCAACC TTGACATTTC CATTTCTTGA CAAAATGCCT 
GAAACCGACC AACTACATTT GCCAAATCTC AATTCTCAAG CGGATTCCCC AAGCAGTGAG 
AAGTCCCCCG CAAGCACGCC TTTTAAGTTC TGGGCGTGGG ACCCGGAAGA GGAGCGTAGG 
CGACAGGAGA AGTGGCAGCA GGAGCAAGAG CGCCTGCTCC AGGAGAGGTA TCAGAAGGAG 
CAGGATAAGC TGAAGGAGGA ATGGGAAAAG GCCCAGAAGG AGGTAGAAGA AGAGGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAGGACACCG TGGTTCCATT CACTATTTCC 
TCGAGTTCTG CCGACCAGCT GTCTACATCC TCATCTGTAA CTGAAGGCAG CGGGACAAGG 
AATAAGATGG ACTTGGAAAA CTGCCAAGAC AGAGACAAAG AGAGAAGACA GAACACTCCC 
CTCCAGGAGA ATGACAGTGA CTCATCACTC AAAGCTAGAG AAAGTGGCCT GCCCGAGGAG 
CACAGCAGCC TAACTCAAAG TCCCTCAGCT AACTCTGAAA ACTCCGTGTC AAAAGGAATT 
AGTCAGGACC AGCAGCCAGA GACAGAGGCT GAGGCCTCAC ACTGTGGTAC AAACCCACAG 
TCAGCTCAGG ATCCACCTTG GAATCAACAG ATCTCAAACC CACCGACATC CAAGTCGGAA 
GACGTGAAGC CCAAAACCCT AGCCCTGGAG AAAAGCATTA ATCATCAGAT TGAGTCTCCG 
GGGGAAAGGC GGAAGTCTAT AAGCGGGAAG AAGCTGTGCT CCTCCTGTGG GCTTGCTTTG 
GGGAAAGGCG CCGCAATGAT CATCGAGACT CTGAATCTCT ACTTTCACAT ACAGTGTTTC 
AGGTGCGGCA TTTGTAAAGG ACAGCTAGGA GATGCAGTGA GTGGGACAGA TGTCAGGATT 
CGCAACGGTC TTCTAAACTG TACCGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCT 
ACAACACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454681.1
CDS	495..3206
Translation	MDPERQETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599192.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599192.1

ORF Insert Sequence:

ATGGATCCTG AAAGACAGGA GACTGATGAC ATTGAAAGCC CCAAGCGCAG CATCCGAGAC 
AGTGGCTACA TCGACTGCTG GGACTCTGAA CGCAGTGACT CCCTTTCTCC TCCTCGCCAC 
GGCCGGGATG ACTCCTTTGA CAGCCTGGAT TCTTTTGGCT CCCGCTCCAG GCAGACACCT 
TCACCAGATG TAGTTCTCAG GGGAAGCAGT GATGGAAGAG GAAGTGATTC TGAATCAGAC 
TTGCCCCACC GGAAGTTGCC AGATGTGAAG AAAGATGATA TGTCCGCACG ACGGACTTCT 
CATGGTGAGC CGAAATCAGC AGTGCCTTTT AACCAGTACC TCCCGAACAA AAGCAACCAG 
ACAGCCTATG TCCCAGCCCC TCTGAGAAAG AAGAAGGCGG AGCGGGAAGA ATACAGGAAG 
AGCTGGAGTA CGGCCACCTC CCCCCTGGGC GGGGAAAGGC CCTTCAGATA CGGTCCGAGA 
ACTCCTGTGT CAGATGACGC AGAGAGCACG AGTATGTTTG ACATGCGGTG TGAGGAGGAG 
GCAGCGGTGC TGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GCTGGAAGAG CCGTCGAAGA 
AGCGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTGATG 
TCTGGAGAAG ATGGGACAAG TGAACGGAGG AAAAGCATCA AAACTTACAG AGAAATTGTC 
CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CACGAGGCAT ATAAGAATGC AAGGTCACAG 
GAGGAGGCAG AGGGGATCCT CCAGCAATAC ATCGAGAGGT TCACCATCAG TGAGGCTGTC 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAACGTA 
TCCTCCTTCC CCAACGACCC CAGCCCCATG AAATACTTGC GTCAGCAGTC ACTGCCCCCG 
CCCAAGTTCA CTGCCACGGT TGAGACTACC ATCGCTCGCA CCAGTGTCCC AGAGAGCAGC 
GTAGCAGCAG GCACTGGGTC TCCAAGCAAA ACCAGCACTC CAAACACGGT GCCTATGCTG 
ACACCCAGGC CTTACTCCCA GCCCAAAAAC TCTCAAGAGG TTCTGAAGAC TTTTAAGGTC 
GATGGGAAAG TCAGCATGAA TGGAGAAACG GCCCGTGGAG ATGTGGAAGG AAAGGAAAAA 
GAAGACCCCA CAGCGGTGGC CCCTGGCCCT TCCTTAACCA AGTCCCAGAT GTTTGAAGGC 
GTGGCCACGG TGCATGGCTC TCCCGTGCAA GTGAAACAAG GCAGCAACAG CATCGAGATC 
AACATCAAGA AGCCAAATTC TCCTCCCCAG GAACTGACAG CAGCCTCTGA GGAAACTGAG 
TCAAACGGCC GAGATGATGA GAACGGTGAA GAGAGCTCAG GGGCACGGGA TGTGGAGCTG 
GATTCGGCAG AGCCACAGCA TTTTACAACA ACCGTGACTC GGTGCAGCCC AACCGTGGCC 
CTGGTGGAGT TTTCTTCCAG CCCGCAGCTG AGGAATGAAG TACCAGAAGA ACAAGACCAG 
AAGAAGCCAG AAAACGAGAT GAGCGGGAAG GTGGAGTTAG TACTGTCCCA GAAGGTGGCA 
AAGCCAAAAT CCCCAGAACC GGAAGCAACC TTGACATTTC CATTTCTTGA CAAAATGCCT 
GAAACCGACC AACTACATTT GCCAAATCTC AATTCTCAAG CGGATTCCCC AAGCAGTGAG 
AAGTCCCCCG CAAGCACGCC TTTTAAGTTC TGGGCGTGGG ACCCGGAAGA GGAGCGTAGG 
CGACAGGAGA AGTGGCAGCA GGAGCAAGAG CGCCTGCTCC AGGAGAGGTA TCAGAAGGAG 
CAGGATAAGC TGAAGGAGGA ATGGGAAAAG GCCCAGAAGG AGGTAGAAGA AGAGGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAGGACACCG TGGTTCCATT CACTATTTCC 
TCGAGTTCTG CCGACCAGCT GTCTACATCC TCATCTGTAA CTGAAGGCAG CGGGACAAGG 
AATAAGATGG ACTTGGAAAA CTGCCAAGAC AGAGACAAAG AGAGAAGACA GAACACTCCC 
CTCCAGGAGA ATGACAGTGA CTCATCACTC AAAGCTAGAG AAAGTGGCCT GCCCGAGGAG 
CACAGCAGCC TAACTCAAAG TCCCTCAGCT AACTCTGAAA ACTCCGTGTC AAAAGGAATT 
AGTCAGGACC AGCAGCCAGA GACAGAGGCT GAGGCCTCAC ACTGTGGTAC AAACCCACAG 
TCAGCTCAGG ATCCACCTTG GAATCAACAG ATCTCAAACC CACCGACATC CAAGTCGGAA 
GACGTGAAGC CCAAAACCCT AGCCCTGGAG AAAAGCATTA ATCATCAGAT TGAGTCTCCG 
GGGGAAAGGC GGAAGTCTAT AAGCGGGAAG AAGCTGTGCT CCTCCTGTGG GCTTGCTTTG 
GGGAAAGGCG CCGCAATGAT CATCGAGACT CTGAATCTCT ACTTTCACAT ACAGTGTTTC 
AGGTGCGGCA TTTGTAAAGG ACAGCTAGGA GATGCAGTGA GTGGGACAGA TGTCAGGATT 
CGCAACGGTC TTCTAAACTG TACCGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCT 
ACAACACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( XM_017599191.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454680.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa46262
Clone ID Related Accession (Same CDS sequence)	XM_017599191.1
Accession Version	XM_017599191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAAGACAGGA GACTGATGAC ATTGAAAGCC CCAAGCGCAG CATCCGAGAC 
AGTGGCTACA TCGACTGCTG GGACTCTGAA CGCAGTGACT CCCTTTCTCC TCCTCGCCAC 
GGCCGGGATG ACTCCTTTGA CAGCCTGGAT TCTTTTGGCT CCCGCTCCAG GCAGACACCT 
TCACCAGATG TAGTTCTCAG GGGAAGCAGT GATGGAAGAG GAAGTGATTC TGAATCAGAC 
TTGCCCCACC GGAAGTTGCC AGATGTGAAG AAAGATGATA TGTCCGCACG ACGGACTTCT 
CATGGTGAGC CGAAATCAGC AGTGCCTTTT AACCAGTACC TCCCGAACAA AAGCAACCAG 
ACAGCCTATG TCCCAGCCCC TCTGAGAAAG AAGAAGGCGG AGCGGGAAGA ATACAGGAAG 
AGCTGGAGTA CGGCCACCTC CCCCCTGGGC GGGGAAAGGC CCTTCAGATA CGGTCCGAGA 
ACTCCTGTGT CAGATGACGC AGAGAGCACG AGTATGTTTG ACATGCGGTG TGAGGAGGAG 
GCAGCGGTGC TGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GCTGGAAGAG CCGTCGAAGA 
AGCGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTGATG 
TCTGGAGAAG ATGGGACAAG TGAACGGAGG AAAAGCATCA AAACTTACAG AGAAATTGTC 
CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CACGAGGCAT ATAAGAATGC AAGGTCACAG 
GAGGAGGCAG AGGGGATCCT CCAGCAATAC ATCGAGAGGT TCACCATCAG TGAGGCTGTC 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAACGTA 
TCCTCCTTCC CCAACGACCC CAGCCCCATG AAATACTTGC GTCAGCAGTC ACTGCCCCCG 
CCCAAGTTCA CTGCCACGGT TGAGACTACC ATCGCTCGCA CCAGTGTCCC AGAGAGCAGC 
GTAGCAGCAG GCACTGGGTC TCCAAGCAAA ACCAGCACTC CAAACACGGT GCCTATGCTG 
ACACCCAGGC CTTACTCCCA GCCCAAAAAC TCTCAAGAGG TTCTGAAGAC TTTTAAGGTC 
GATGGGAAAG TCAGCATGAA TGGAGAAACG GCCCGTGGAG ATGTGGAAGG AAAGGAAAAA 
GAAGACCCCA CAGCGGTGGC CCCTGGCCCT TCCTTAACCA AGTCCCAGAT GTTTGAAGGC 
GTGGCCACGG TGCATGGCTC TCCCGTGCAA GTGAAACAAG GCAGCAACAG CATCGAGATC 
AACATCAAGA AGCCAAATTC TCCTCCCCAG GAACTGACAG CAGCCTCTGA GGAAACTGAG 
TCAAACGGCC GAGATGATGA GAACGGTGAA GAGAGCTCAG GGGCACGGGA TGTGGAGCTG 
GATTCGGCAG AGCCACAGCA TTTTACAACA ACCGTGACTC GGTGCAGCCC AACCGTGGCC 
CTGGTGGAGT TTTCTTCCAG CCCGCAGCTG AGGAATGAAG TACCAGAAGA ACAAGACCAG 
AAGAAGCCAG AAAACGAGAT GAGCGGGAAG GTGGAGTTAG TACTGTCCCA GAAGGTGGCA 
AAGCCAAAAT CCCCAGAACC GGAAGCAACC TTGACATTTC CATTTCTTGA CAAAATGCCT 
GAAACCGACC AACTACATTT GCCAAATCTC AATTCTCAAG CGGATTCCCC AAGCAGTGAG 
AAGTCCCCCG CAAGCACGCC TTTTAAGTTC TGGGCGTGGG ACCCGGAAGA GGAGCGTAGG 
CGACAGGAGA AGTGGCAGCA GGAGCAAGAG CGCCTGCTCC AGGAGAGGTA TCAGAAGGAG 
CAGGATAAGC TGAAGGAGGA ATGGGAAAAG GCCCAGAAGG AGGTAGAAGA AGAGGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAGGACACCG TGGTTCCATT CACTATTTCC 
TCGAGTTCTG CCGACCAGCT GTCTACATCC TCATCTGTAA CTGAAGGCAG CGGGACAAGG 
AATAAGATGG ACTTGGAAAA CTGCCAAGAC AGAGACAAAG AGAGAAGACA GAACACTCCC 
CTCCAGGAGA ATGACAGTGA CTCATCACTC AAAGCTAGAG AAAGTGGCCT GCCCGAGGAG 
CACAGCAGCC TAACTCAAAG TCCCTCAGCT AACTCTGAAA ACTCCGTGTC AAAAGGAATT 
AGTCAGGACC AGCAGCCAGA GACAGAGGCT GAGGCCTCAC ACTGTGGTAC AAACCCACAG 
TCAGCTCAGG ATCCACCTTG GAATCAACAG ATCTCAAACC CACCGACATC CAAGTCGGAA 
GACGTGAAGC CCAAAACCCT AGCCCTGGAG AAAAGCATTA ATCATCAGAT TGAGTCTCCG 
GGGGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCGGG CTGGGCCCCT GTCCCCCTGC 
TCTCCTACCC CTCCTGGCCA GTCTCCAAAC AGGTACAAGA GGTCTATAAG CGGGAAGAAG 
CTGTGCTCCT CCTGTGGGCT TGCTTTGGGG AAAGGCGCCG CAATGATCAT CGAGACTCTG 
AATCTCTACT TTCACATACA GTGTTTCAGG TGCGGCATTT GTAAAGGACA GCTAGGAGAT 
GCAGTGAGTG GGACAGATGT CAGGATTCGC AACGGTCTTC TAAACTGTAC CGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCTACA ACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454680.1
CDS	499..3297
Translation	MDPERQETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKKSPRENFRAGPLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599191.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599191.1

ORF Insert Sequence:

ATGGATCCTG AAAGACAGGA GACTGATGAC ATTGAAAGCC CCAAGCGCAG CATCCGAGAC 
AGTGGCTACA TCGACTGCTG GGACTCTGAA CGCAGTGACT CCCTTTCTCC TCCTCGCCAC 
GGCCGGGATG ACTCCTTTGA CAGCCTGGAT TCTTTTGGCT CCCGCTCCAG GCAGACACCT 
TCACCAGATG TAGTTCTCAG GGGAAGCAGT GATGGAAGAG GAAGTGATTC TGAATCAGAC 
TTGCCCCACC GGAAGTTGCC AGATGTGAAG AAAGATGATA TGTCCGCACG ACGGACTTCT 
CATGGTGAGC CGAAATCAGC AGTGCCTTTT AACCAGTACC TCCCGAACAA AAGCAACCAG 
ACAGCCTATG TCCCAGCCCC TCTGAGAAAG AAGAAGGCGG AGCGGGAAGA ATACAGGAAG 
AGCTGGAGTA CGGCCACCTC CCCCCTGGGC GGGGAAAGGC CCTTCAGATA CGGTCCGAGA 
ACTCCTGTGT CAGATGACGC AGAGAGCACG AGTATGTTTG ACATGCGGTG TGAGGAGGAG 
GCAGCGGTGC TGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GCTGGAAGAG CCGTCGAAGA 
AGCGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTGATG 
TCTGGAGAAG ATGGGACAAG TGAACGGAGG AAAAGCATCA AAACTTACAG AGAAATTGTC 
CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CACGAGGCAT ATAAGAATGC AAGGTCACAG 
GAGGAGGCAG AGGGGATCCT CCAGCAATAC ATCGAGAGGT TCACCATCAG TGAGGCTGTC 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAACGTA 
TCCTCCTTCC CCAACGACCC CAGCCCCATG AAATACTTGC GTCAGCAGTC ACTGCCCCCG 
CCCAAGTTCA CTGCCACGGT TGAGACTACC ATCGCTCGCA CCAGTGTCCC AGAGAGCAGC 
GTAGCAGCAG GCACTGGGTC TCCAAGCAAA ACCAGCACTC CAAACACGGT GCCTATGCTG 
ACACCCAGGC CTTACTCCCA GCCCAAAAAC TCTCAAGAGG TTCTGAAGAC TTTTAAGGTC 
GATGGGAAAG TCAGCATGAA TGGAGAAACG GCCCGTGGAG ATGTGGAAGG AAAGGAAAAA 
GAAGACCCCA CAGCGGTGGC CCCTGGCCCT TCCTTAACCA AGTCCCAGAT GTTTGAAGGC 
GTGGCCACGG TGCATGGCTC TCCCGTGCAA GTGAAACAAG GCAGCAACAG CATCGAGATC 
AACATCAAGA AGCCAAATTC TCCTCCCCAG GAACTGACAG CAGCCTCTGA GGAAACTGAG 
TCAAACGGCC GAGATGATGA GAACGGTGAA GAGAGCTCAG GGGCACGGGA TGTGGAGCTG 
GATTCGGCAG AGCCACAGCA TTTTACAACA ACCGTGACTC GGTGCAGCCC AACCGTGGCC 
CTGGTGGAGT TTTCTTCCAG CCCGCAGCTG AGGAATGAAG TACCAGAAGA ACAAGACCAG 
AAGAAGCCAG AAAACGAGAT GAGCGGGAAG GTGGAGTTAG TACTGTCCCA GAAGGTGGCA 
AAGCCAAAAT CCCCAGAACC GGAAGCAACC TTGACATTTC CATTTCTTGA CAAAATGCCT 
GAAACCGACC AACTACATTT GCCAAATCTC AATTCTCAAG CGGATTCCCC AAGCAGTGAG 
AAGTCCCCCG CAAGCACGCC TTTTAAGTTC TGGGCGTGGG ACCCGGAAGA GGAGCGTAGG 
CGACAGGAGA AGTGGCAGCA GGAGCAAGAG CGCCTGCTCC AGGAGAGGTA TCAGAAGGAG 
CAGGATAAGC TGAAGGAGGA ATGGGAAAAG GCCCAGAAGG AGGTAGAAGA AGAGGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAGGACACCG TGGTTCCATT CACTATTTCC 
TCGAGTTCTG CCGACCAGCT GTCTACATCC TCATCTGTAA CTGAAGGCAG CGGGACAAGG 
AATAAGATGG ACTTGGAAAA CTGCCAAGAC AGAGACAAAG AGAGAAGACA GAACACTCCC 
CTCCAGGAGA ATGACAGTGA CTCATCACTC AAAGCTAGAG AAAGTGGCCT GCCCGAGGAG 
CACAGCAGCC TAACTCAAAG TCCCTCAGCT AACTCTGAAA ACTCCGTGTC AAAAGGAATT 
AGTCAGGACC AGCAGCCAGA GACAGAGGCT GAGGCCTCAC ACTGTGGTAC AAACCCACAG 
TCAGCTCAGG ATCCACCTTG GAATCAACAG ATCTCAAACC CACCGACATC CAAGTCGGAA 
GACGTGAAGC CCAAAACCCT AGCCCTGGAG AAAAGCATTA ATCATCAGAT TGAGTCTCCG 
GGGGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCGGG CTGGGCCCCT GTCCCCCTGC 
TCTCCTACCC CTCCTGGCCA GTCTCCAAAC AGGTACAAGA GGTCTATAAG CGGGAAGAAG 
CTGTGCTCCT CCTGTGGGCT TGCTTTGGGG AAAGGCGCCG CAATGATCAT CGAGACTCTG 
AATCTCTACT TTCACATACA GTGTTTCAGG TGCGGCATTT GTAAAGGACA GCTAGGAGAT 
GCAGTGAGTG GGACAGATGT CAGGATTCGC AACGGTCTTC TAAACTGTAC CGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCTACA ACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( XM_017599187.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454676.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58290
Clone ID Related Accession (Same CDS sequence)	XM_017599187.1
Accession Version	XM_017599187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGAAAAGCC CTCGAGAGAA CTTCCGGGCT GGGCCCCTGT CCCCCTGCTC TCCTACCCCT 
CCTGGCCAGT CTCCAAACAG GTACAAGAGG TCTATAAGCG GGAAGAAGCT GTGCTCCTCC 
TGTGGGCTTG CTTTGGGGAA AGGCGCCGCA ATGATCATCG AGACTCTGAA TCTCTACTTT 
CACATACAGT GTTTCAGGTG CGGCATTTGT AAAGGACAGC TAGGAGATGC AGTGAGTGGG 
ACAGATGTCA GGATTCGCAA CGGTCTTCTA AACTGTACCG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCTACAAC ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454676.1
CDS	134..3400
Translation	MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKKSPRENFRAGPLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599187.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599187.1

ORF Insert Sequence:

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGAAAAGCC CTCGAGAGAA CTTCCGGGCT GGGCCCCTGT CCCCCTGCTC TCCTACCCCT 
CCTGGCCAGT CTCCAAACAG GTACAAGAGG TCTATAAGCG GGAAGAAGCT GTGCTCCTCC 
TGTGGGCTTG CTTTGGGGAA AGGCGCCGCA ATGATCATCG AGACTCTGAA TCTCTACTTT 
CACATACAGT GTTTCAGGTG CGGCATTTGT AAAGGACAGC TAGGAGATGC AGTGAGTGGG 
ACAGATGTCA GGATTCGCAA CGGTCTTCTA AACTGTACCG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCTACAAC ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( XM_017599188.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454677.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa58291
Clone ID Related Accession (Same CDS sequence)	XM_017599188.1
Accession Version	XM_017599188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAGG AGGCAGCGGT GCTGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGCTGGAAG 
AGCCGTCGAA GAAGCGCTTC CCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTGA TGTCTGGAGA AGATGGGACA AGTGAACGGA GGAAAAGCAT CAAAACTTAC 
AGAGAAATTG TCCAAGAAAA GGAGCGGAGA GAGAGGGAGC TGCACGAGGC ATATAAGAAT 
GCAAGGTCAC AGGAGGAGGC AGAGGGGATC CTCCAGCAAT ACATCGAGAG GTTCACCATC 
AGTGAGGCTG TCCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAACG TATCCTCCTT CCCCAACGAC CCCAGCCCCA TGAAATACTT GCGTCAGCAG 
TCACTGCCCC CGCCCAAGTT CACTGCCACG GTTGAGACTA CCATCGCTCG CACCAGTGTC 
CCAGAGAGCA GCGTAGCAGC AGGCACTGGG TCTCCAAGCA AAACCAGCAC TCCAAACACG 
GTGCCTATGC TGACACCCAG GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTTCTGAAG 
ACTTTTAAGG TCGATGGGAA AGTCAGCATG AATGGAGAAA CGGCCCGTGG AGATGTGGAA 
GGAAAGGAAA AAGAAGACCC CACAGCGGTG GCCCCTGGCC CTTCCTTAAC CAAGTCCCAG 
ATGTTTGAAG GCGTGGCCAC GGTGCATGGC TCTCCCGTGC AAGTGAAACA AGGCAGCAAC 
AGCATCGAGA TCAACATCAA GAAGCCAAAT TCTCCTCCCC AGGAACTGAC AGCAGCCTCT 
GAGGAAACTG AGTCAAACGG CCGAGATGAT GAGAACGGTG AAGAGAGCTC AGGGGCACGG 
GATGTGGAGC TGGATTCGGC AGAGCCACAG CATTTTACAA CAACCGTGAC TCGGTGCAGC 
CCAACCGTGG CCCTGGTGGA GTTTTCTTCC AGCCCGCAGC TGAGGAATGA AGTACCAGAA 
GAACAAGACC AGAAGAAGCC AGAAAACGAG ATGAGCGGGA AGGTGGAGTT AGTACTGTCC 
CAGAAGGTGG CAAAGCCAAA ATCCCCAGAA CCGGAAGCAA CCTTGACATT TCCATTTCTT 
GACAAAATGC CTGAAACCGA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGCTGCCT 
GGCAGTGCCC CGCTGAGAGT TCAGAACTCT TGGCGACGGT CCCAGTTTTT CTCCCAGTCA 
GCGGATTCCC CAAGCAGTGA GAAGTCCCCC GCAAGCACGC CTTTTAAGTT CTGGGCGTGG 
GACCCGGAAG AGGAGCGTAG GCGACAGGAG AAGTGGCAGC AGGAGCAAGA GCGCCTGCTC 
CAGGAGAGGT ATCAGAAGGA GCAGGATAAG CTGAAGGAGG AATGGGAAAA GGCCCAGAAG 
GAGGTAGAAG AAGAGGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAGGACACC 
GTGGTTCCAT TCACTATTTC CTCGAGTTCT GCCGACCAGC TGTCTACATC CTCATCTGTA 
ACTGAAGGCA GCGGGACAAG GAATAAGATG GACTTGGAAA ACTGCCAAGA CAGAGACAAA 
GAGAGAAGAC AGAACACTCC CCTCCAGGAG AATGACAGTG ACTCATCACT CAAAGCTAGA 
GAAAGTGGCC TGCCCGAGGA GCACAGCAGC CTAACTCAAA GTCCCTCAGC TAACTCTGAA 
AACTCCGTGT CAAAAGGAAT TAGTCAGGAC CAGCAGCCAG AGACAGAGGC TGAGGCCTCA 
CACTGTGGTA CAAACCCACA GTCAGCTCAG GATCCACCTT GGAATCAACA GATCTCAAAC 
CCACCGACAT CCAAGTCGGA AGACGTGAAG CCCAAAACCC TAGCCCTGGA GAAAAGCATT 
AATCATCAGA TTGAGTCTCC GGGGGAAAGG CGGAAGTCTA TAAGCGGGAA GAAGCTGTGC 
TCCTCCTGTG GGCTTGCTTT GGGGAAAGGC GCCGCAATGA TCATCGAGAC TCTGAATCTC 
TACTTTCACA TACAGTGTTT CAGGTGCGGC ATTTGTAAAG GACAGCTAGG AGATGCAGTG 
AGTGGGACAG ATGTCAGGAT TCGCAACGGT CTTCTAAACT GTACCGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC TACAACACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454677.1
CDS	134..3346
Translation	MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQELPGSAPLRVQNSWRRSQFFSQSADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599188.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599188.1

ORF Insert Sequence:

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAGG AGGCAGCGGT GCTGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGCTGGAAG 
AGCCGTCGAA GAAGCGCTTC CCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTGA TGTCTGGAGA AGATGGGACA AGTGAACGGA GGAAAAGCAT CAAAACTTAC 
AGAGAAATTG TCCAAGAAAA GGAGCGGAGA GAGAGGGAGC TGCACGAGGC ATATAAGAAT 
GCAAGGTCAC AGGAGGAGGC AGAGGGGATC CTCCAGCAAT ACATCGAGAG GTTCACCATC 
AGTGAGGCTG TCCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAACG TATCCTCCTT CCCCAACGAC CCCAGCCCCA TGAAATACTT GCGTCAGCAG 
TCACTGCCCC CGCCCAAGTT CACTGCCACG GTTGAGACTA CCATCGCTCG CACCAGTGTC 
CCAGAGAGCA GCGTAGCAGC AGGCACTGGG TCTCCAAGCA AAACCAGCAC TCCAAACACG 
GTGCCTATGC TGACACCCAG GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTTCTGAAG 
ACTTTTAAGG TCGATGGGAA AGTCAGCATG AATGGAGAAA CGGCCCGTGG AGATGTGGAA 
GGAAAGGAAA AAGAAGACCC CACAGCGGTG GCCCCTGGCC CTTCCTTAAC CAAGTCCCAG 
ATGTTTGAAG GCGTGGCCAC GGTGCATGGC TCTCCCGTGC AAGTGAAACA AGGCAGCAAC 
AGCATCGAGA TCAACATCAA GAAGCCAAAT TCTCCTCCCC AGGAACTGAC AGCAGCCTCT 
GAGGAAACTG AGTCAAACGG CCGAGATGAT GAGAACGGTG AAGAGAGCTC AGGGGCACGG 
GATGTGGAGC TGGATTCGGC AGAGCCACAG CATTTTACAA CAACCGTGAC TCGGTGCAGC 
CCAACCGTGG CCCTGGTGGA GTTTTCTTCC AGCCCGCAGC TGAGGAATGA AGTACCAGAA 
GAACAAGACC AGAAGAAGCC AGAAAACGAG ATGAGCGGGA AGGTGGAGTT AGTACTGTCC 
CAGAAGGTGG CAAAGCCAAA ATCCCCAGAA CCGGAAGCAA CCTTGACATT TCCATTTCTT 
GACAAAATGC CTGAAACCGA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGCTGCCT 
GGCAGTGCCC CGCTGAGAGT TCAGAACTCT TGGCGACGGT CCCAGTTTTT CTCCCAGTCA 
GCGGATTCCC CAAGCAGTGA GAAGTCCCCC GCAAGCACGC CTTTTAAGTT CTGGGCGTGG 
GACCCGGAAG AGGAGCGTAG GCGACAGGAG AAGTGGCAGC AGGAGCAAGA GCGCCTGCTC 
CAGGAGAGGT ATCAGAAGGA GCAGGATAAG CTGAAGGAGG AATGGGAAAA GGCCCAGAAG 
GAGGTAGAAG AAGAGGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAGGACACC 
GTGGTTCCAT TCACTATTTC CTCGAGTTCT GCCGACCAGC TGTCTACATC CTCATCTGTA 
ACTGAAGGCA GCGGGACAAG GAATAAGATG GACTTGGAAA ACTGCCAAGA CAGAGACAAA 
GAGAGAAGAC AGAACACTCC CCTCCAGGAG AATGACAGTG ACTCATCACT CAAAGCTAGA 
GAAAGTGGCC TGCCCGAGGA GCACAGCAGC CTAACTCAAA GTCCCTCAGC TAACTCTGAA 
AACTCCGTGT CAAAAGGAAT TAGTCAGGAC CAGCAGCCAG AGACAGAGGC TGAGGCCTCA 
CACTGTGGTA CAAACCCACA GTCAGCTCAG GATCCACCTT GGAATCAACA GATCTCAAAC 
CCACCGACAT CCAAGTCGGA AGACGTGAAG CCCAAAACCC TAGCCCTGGA GAAAAGCATT 
AATCATCAGA TTGAGTCTCC GGGGGAAAGG CGGAAGTCTA TAAGCGGGAA GAAGCTGTGC 
TCCTCCTGTG GGCTTGCTTT GGGGAAAGGC GCCGCAATGA TCATCGAGAC TCTGAATCTC 
TACTTTCACA TACAGTGTTT CAGGTGCGGC ATTTGTAAAG GACAGCTAGG AGATGCAGTG 
AGTGGGACAG ATGTCAGGAT TCGCAACGGT CTTCTAAACT GTACCGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC TACAACACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( XM_017599189.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454678.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa58292
Clone ID Related Accession (Same CDS sequence)	XM_017599189.1
Accession Version	XM_017599189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGTCTATAA GCGGGAAGAA GCTGTGCTCC TCCTGTGGGC TTGCTTTGGG GAAAGGCGCC 
GCAATGATCA TCGAGACTCT GAATCTCTAC TTTCACATAC AGTGTTTCAG GTGCGGCATT 
TGTAAAGGAC AGCTAGGAGA TGCAGTGAGT GGGACAGATG TCAGGATTCG CAACGGTCTT 
CTAAACTGTA CCGACTGCTA CATGCGATCC AGAAGTGCTG GCCAGCCTAC AACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454678.1
CDS	134..3313
Translation	MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599189.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599189.1

ORF Insert Sequence:

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGTCTATAA GCGGGAAGAA GCTGTGCTCC TCCTGTGGGC TTGCTTTGGG GAAAGGCGCC 
GCAATGATCA TCGAGACTCT GAATCTCTAC TTTCACATAC AGTGTTTCAG GTGCGGCATT 
TGTAAAGGAC AGCTAGGAGA TGCAGTGAGT GGGACAGATG TCAGGATTCG CAACGGTCTT 
CTAAACTGTA CCGACTGCTA CATGCGATCC AGAAGTGCTG GCCAGCCTAC AACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( XM_017599190.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454679.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa58293
Clone ID Related Accession (Same CDS sequence)	XM_017599190.1
Accession Version	XM_017599190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAGG AGGCAGCGGT GCTGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGCTGGAAG 
AGCCGTCGAA GAAGCGCTTC CCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTGA TGTCTGGAGA AGATGGGACA AGTGAACGGA GGAAAAGCAT CAAAACTTAC 
AGAGAAATTG TCCAAGAAAA GGAGCGGAGA GAGAGGGAGC TGCACGAGGC ATATAAGAAT 
GCAAGGTCAC AGGAGGAGGC AGAGGGGATC CTCCAGCAAT ACATCGAGAG GTTCACCATC 
AGTGAGGCTG TCCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAACG TATCCTCCTT CCCCAACGAC CCCAGCCCCA TGAAATACTT GCGTCAGCAG 
TCACTGCCCC CGCCCAAGTT CACTGCCACG GTTGAGACTA CCATCGCTCG CACCAGTGTC 
CCAGAGAGCA GCGTAGCAGC AGGCACTGGG TCTCCAAGCA AAACCAGCAC TCCAAACACG 
GTGCCTATGC TGACACCCAG GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTTCTGAAG 
ACTTTTAAGG TCGATGGGAA AGTCAGCATG AATGGAGAAA CGGCCCGTGG AGATGTGGAA 
GGAAAGGAAA AAGAAGACCC CACAGCGGTG GCCCCTGGCC CTTCCTTAAC CAAGTCCCAG 
ATGTTTGAAG GCGTGGCCAC GGTGCATGGC TCTCCCGTGC AAGTGAAACA AGGCAGCAAC 
AGCATCGAGA TCAACATCAA GAAGCCAAAT TCTCCTCCCC AGGAACTGAC AGCAGCCTCT 
GAGGAAACTG AGTCAAACGG CCGAGATGAT GAGAACGGTG AAGAGAGCTC AGGGGCACGG 
GATGTGGAGC TGGATTCGGC AGAGCCACAG CATTTTACAA CAACCGTGAC TCGGTGCAGC 
CCAACCGTGG CCCTGGTGGA GTTTTCTTCC AGCCCGCAGC TGAGGAATGA AGTACCAGAA 
GAACAAGACC AGAAGAAGCC AGAAAACGAG ATGAGCGGGA AGGTGGAGTT AGTACTGTCC 
CAGAAGGTGG CAAAGCCAAA ATCCCCAGAA CCGGAAGCAA CCTTGACATT TCCATTTCTT 
GACAAAATGC CTGAAACCGA CCAACTACAT TTGCCAAATC TCAATTCTCA AGCGGATTCC 
CCAAGCAGTG AGAAGTCCCC CGCAAGCACG CCTTTTAAGT TCTGGGCGTG GGACCCGGAA 
GAGGAGCGTA GGCGACAGGA GAAGTGGCAG CAGGAGCAAG AGCGCCTGCT CCAGGAGAGG 
TATCAGAAGG AGCAGGATAA GCTGAAGGAG GAATGGGAAA AGGCCCAGAA GGAGGTAGAA 
GAAGAGGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAGGACAC CGTGGTTCCA 
TTCACTATTT CCTCGAGTTC TGCCGACCAG CTGTCTACAT CCTCATCTGT AACTGAAGGC 
AGCGGGACAA GGAATAAGAT GGACTTGGAA AACTGCCAAG ACAGAGACAA AGAGAGAAGA 
CAGAACACTC CCCTCCAGGA GAATGACAGT GACTCATCAC TCAAAGCTAG AGAAAGTGGC 
CTGCCCGAGG AGCACAGCAG CCTAACTCAA AGTCCCTCAG CTAACTCTGA AAACTCCGTG 
TCAAAAGGAA TTAGTCAGGA CCAGCAGCCA GAGACAGAGG CTGAGGCCTC ACACTGTGGT 
ACAAACCCAC AGTCAGCTCA GGATCCACCT TGGAATCAAC AGATCTCAAA CCCACCGACA 
TCCAAGTCGG AAGACGTGAA GCCCAAAACC CTAGCCCTGG AGAAAAGCAT TAATCATCAG 
ATTGAGTCTC CGGGGGAAAG GCGGAAGTCT ATAAGCGGGA AGAAGCTGTG CTCCTCCTGT 
GGGCTTGCTT TGGGGAAAGG CGCCGCAATG ATCATCGAGA CTCTGAATCT CTACTTTCAC 
ATACAGTGTT TCAGGTGCGG CATTTGTAAA GGACAGCTAG GAGATGCAGT GAGTGGGACA 
GATGTCAGGA TTCGCAACGG TCTTCTAAAC TGTACCGACT GCTACATGCG ATCCAGAAGT 
GCTGGCCAGC CTACAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454679.1
CDS	134..3277
Translation	MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017599190.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599190.1

ORF Insert Sequence:

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAGG AGGCAGCGGT GCTGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGCTGGAAG 
AGCCGTCGAA GAAGCGCTTC CCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTGA TGTCTGGAGA AGATGGGACA AGTGAACGGA GGAAAAGCAT CAAAACTTAC 
AGAGAAATTG TCCAAGAAAA GGAGCGGAGA GAGAGGGAGC TGCACGAGGC ATATAAGAAT 
GCAAGGTCAC AGGAGGAGGC AGAGGGGATC CTCCAGCAAT ACATCGAGAG GTTCACCATC 
AGTGAGGCTG TCCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAACG TATCCTCCTT CCCCAACGAC CCCAGCCCCA TGAAATACTT GCGTCAGCAG 
TCACTGCCCC CGCCCAAGTT CACTGCCACG GTTGAGACTA CCATCGCTCG CACCAGTGTC 
CCAGAGAGCA GCGTAGCAGC AGGCACTGGG TCTCCAAGCA AAACCAGCAC TCCAAACACG 
GTGCCTATGC TGACACCCAG GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTTCTGAAG 
ACTTTTAAGG TCGATGGGAA AGTCAGCATG AATGGAGAAA CGGCCCGTGG AGATGTGGAA 
GGAAAGGAAA AAGAAGACCC CACAGCGGTG GCCCCTGGCC CTTCCTTAAC CAAGTCCCAG 
ATGTTTGAAG GCGTGGCCAC GGTGCATGGC TCTCCCGTGC AAGTGAAACA AGGCAGCAAC 
AGCATCGAGA TCAACATCAA GAAGCCAAAT TCTCCTCCCC AGGAACTGAC AGCAGCCTCT 
GAGGAAACTG AGTCAAACGG CCGAGATGAT GAGAACGGTG AAGAGAGCTC AGGGGCACGG 
GATGTGGAGC TGGATTCGGC AGAGCCACAG CATTTTACAA CAACCGTGAC TCGGTGCAGC 
CCAACCGTGG CCCTGGTGGA GTTTTCTTCC AGCCCGCAGC TGAGGAATGA AGTACCAGAA 
GAACAAGACC AGAAGAAGCC AGAAAACGAG ATGAGCGGGA AGGTGGAGTT AGTACTGTCC 
CAGAAGGTGG CAAAGCCAAA ATCCCCAGAA CCGGAAGCAA CCTTGACATT TCCATTTCTT 
GACAAAATGC CTGAAACCGA CCAACTACAT TTGCCAAATC TCAATTCTCA AGCGGATTCC 
CCAAGCAGTG AGAAGTCCCC CGCAAGCACG CCTTTTAAGT TCTGGGCGTG GGACCCGGAA 
GAGGAGCGTA GGCGACAGGA GAAGTGGCAG CAGGAGCAAG AGCGCCTGCT CCAGGAGAGG 
TATCAGAAGG AGCAGGATAA GCTGAAGGAG GAATGGGAAA AGGCCCAGAA GGAGGTAGAA 
GAAGAGGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAGGACAC CGTGGTTCCA 
TTCACTATTT CCTCGAGTTC TGCCGACCAG CTGTCTACAT CCTCATCTGT AACTGAAGGC 
AGCGGGACAA GGAATAAGAT GGACTTGGAA AACTGCCAAG ACAGAGACAA AGAGAGAAGA 
CAGAACACTC CCCTCCAGGA GAATGACAGT GACTCATCAC TCAAAGCTAG AGAAAGTGGC 
CTGCCCGAGG AGCACAGCAG CCTAACTCAA AGTCCCTCAG CTAACTCTGA AAACTCCGTG 
TCAAAAGGAA TTAGTCAGGA CCAGCAGCCA GAGACAGAGG CTGAGGCCTC ACACTGTGGT 
ACAAACCCAC AGTCAGCTCA GGATCCACCT TGGAATCAAC AGATCTCAAA CCCACCGACA 
TCCAAGTCGG AAGACGTGAA GCCCAAAACC CTAGCCCTGG AGAAAAGCAT TAATCATCAG 
ATTGAGTCTC CGGGGGAAAG GCGGAAGTCT ATAAGCGGGA AGAAGCTGTG CTCCTCCTGT 
GGGCTTGCTT TGGGGAAAGG CGCCGCAATG ATCATCGAGA CTCTGAATCT CTACTTTCAC 
ATACAGTGTT TCAGGTGCGG CATTTGTAAA GGACAGCTAG GAGATGCAGT GAGTGGGACA 
GATGTCAGGA TTCGCAACGG TCTTCTAAAC TGTACCGACT GCTACATGCG ATCCAGAAGT 
GCTGGCCAGC CTACAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limch1 ( NM_001191678.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178607.1 Rattus norvegicus LIM and calponin homology domains 1 (Limch1), mRNA.

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CloneID	ORa07380
Clone ID Related Accession (Same CDS sequence)	NM_001191678.1
Accession Version	NM_001191678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	LIM and calponin homology domains-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07014984.1 and AABR07014983.1. On Jul 17, 2010 this sequence version replaced XM_223397.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGAAAAGCC CTCGAGAGAA CTTCCGGGCT GGGCCCCTGT CCCCCTGCTC TCCTACCCCT 
CCTGGCCAGT CTCCAAACAG GTCTATAAGC GGGAAGAAGC TGTGCTCCTC CTGTGGGCTT 
GCTTTGGGGA AAGGCGCCGC AATGATCATC GAGACTCTGA ATCTCTACTT TCACATACAG 
TGTTTCAGGT GCGGCATTTG TAAAGGACAG CTAGGAGATG CAGTGAGTGG GACAGATGTC 
AGGATTCGCA ACGGTCTTCT AAACTGTACC GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCTACAA CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178607.1
CDS	1..3258
Translation	MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETTIARTSVPESSVAAGTGSPSKTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVEGKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETESNGRDDENGEESSGARDVELDSAEPQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQKKPENEMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETDQLHLPNLNSQADSPSSEKSPASTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERRQNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQSAQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKKSPRENFRAGPLSPCSPTPPGQSPNRSISGKKLCSSCGLALGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001191678.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM and calponin homology domains 1 (Limch1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191678.1

ORF Insert Sequence:

ATGGCTTGCC CCGCTCTCGG TCTGGAAGTC CTTCAACCCC TGCAGCCCGA ACCACCTCCG 
GAGCCTGCCT TCGCCGAGGC ACAGAAGTGG ATCGAGCAAG TCACGGGTCG AAGTTTTGGT 
GACAAAGACT TCCGGACAGG ACTAGAGAAT GGAATCCTTC TTTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AACACCATCT TATTCTTGAG AGGCTGTAAA GAGCTTGGCC TTAAAGAATC TCAACTCTTT 
GACCCAAGTG ACCTCCAGGA TACTTCCAAC AGGGTCACTG TCAAGAACTT CGATTATAGC 
AGGAAGCTGA AAAACGTGTT GGTGACCATT TACTGGCTGG GGAAAGCTGC AAATAGCTGC 
ACATCCTACA GTGGAACCAC ACTGAACCTG AAGGAGTTCG AAGGACTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGCCCC AAGCGCAGCA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCTGAACG CAGTGACTCC CTTTCTCCTC CTCGCCACGG CCGGGATGAC 
TCCTTTGACA GCCTGGATTC TTTTGGCTCC CGCTCCAGGC AGACACCTTC ACCAGATGTA 
GTTCTCAGGG GAAGCAGTGA TGGAAGAGGA AGTGATTCTG AATCAGACTT GCCCCACCGG 
AAGTTGCCAG ATGTGAAGAA AGATGATATG TCCGCACGAC GGACTTCTCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAACCAGAC AGCCTATGTC 
CCAGCCCCTC TGAGAAAGAA GAAGGCGGAG CGGGAAGAAT ACAGGAAGAG CTGGAGTACG 
GCCACCTCCC CCCTGGGCGG GGAAAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCA 
GATGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAGGAGGC AGCGGTGCTG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGC TGGAAGAGCC GTCGAAGAAG CGCTTCCCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTGATGTC TGGAGAAGAT 
GGGACAAGTG AACGGAGGAA AAGCATCAAA ACTTACAGAG AAATTGTCCA AGAAAAGGAG 
CGGAGAGAGA GGGAGCTGCA CGAGGCATAT AAGAATGCAA GGTCACAGGA GGAGGCAGAG 
GGGATCCTCC AGCAATACAT CGAGAGGTTC ACCATCAGTG AGGCTGTCCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAACGTATC CTCCTTCCCC 
AACGACCCCA GCCCCATGAA ATACTTGCGT CAGCAGTCAC TGCCCCCGCC CAAGTTCACT 
GCCACGGTTG AGACTACCAT CGCTCGCACC AGTGTCCCAG AGAGCAGCGT AGCAGCAGGC 
ACTGGGTCTC CAAGCAAAAC CAGCACTCCA AACACGGTGC CTATGCTGAC ACCCAGGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTT CTGAAGACTT TTAAGGTCGA TGGGAAAGTC 
AGCATGAATG GAGAAACGGC CCGTGGAGAT GTGGAAGGAA AGGAAAAAGA AGACCCCACA 
GCGGTGGCCC CTGGCCCTTC CTTAACCAAG TCCCAGATGT TTGAAGGCGT GGCCACGGTG 
CATGGCTCTC CCGTGCAAGT GAAACAAGGC AGCAACAGCA TCGAGATCAA CATCAAGAAG 
CCAAATTCTC CTCCCCAGGA ACTGACAGCA GCCTCTGAGG AAACTGAGTC AAACGGCCGA 
GATGATGAGA ACGGTGAAGA GAGCTCAGGG GCACGGGATG TGGAGCTGGA TTCGGCAGAG 
CCACAGCATT TTACAACAAC CGTGACTCGG TGCAGCCCAA CCGTGGCCCT GGTGGAGTTT 
TCTTCCAGCC CGCAGCTGAG GAATGAAGTA CCAGAAGAAC AAGACCAGAA GAAGCCAGAA 
AACGAGATGA GCGGGAAGGT GGAGTTAGTA CTGTCCCAGA AGGTGGCAAA GCCAAAATCC 
CCAGAACCGG AAGCAACCTT GACATTTCCA TTTCTTGACA AAATGCCTGA AACCGACCAA 
CTACATTTGC CAAATCTCAA TTCTCAAGCG GATTCCCCAA GCAGTGAGAA GTCCCCCGCA 
AGCACGCCTT TTAAGTTCTG GGCGTGGGAC CCGGAAGAGG AGCGTAGGCG ACAGGAGAAG 
TGGCAGCAGG AGCAAGAGCG CCTGCTCCAG GAGAGGTATC AGAAGGAGCA GGATAAGCTG 
AAGGAGGAAT GGGAAAAGGC CCAGAAGGAG GTAGAAGAAG AGGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA GGACACCGTG GTTCCATTCA CTATTTCCTC GAGTTCTGCC 
GACCAGCTGT CTACATCCTC ATCTGTAACT GAAGGCAGCG GGACAAGGAA TAAGATGGAC 
TTGGAAAACT GCCAAGACAG AGACAAAGAG AGAAGACAGA ACACTCCCCT CCAGGAGAAT 
GACAGTGACT CATCACTCAA AGCTAGAGAA AGTGGCCTGC CCGAGGAGCA CAGCAGCCTA 
ACTCAAAGTC CCTCAGCTAA CTCTGAAAAC TCCGTGTCAA AAGGAATTAG TCAGGACCAG 
CAGCCAGAGA CAGAGGCTGA GGCCTCACAC TGTGGTACAA ACCCACAGTC AGCTCAGGAT 
CCACCTTGGA ATCAACAGAT CTCAAACCCA CCGACATCCA AGTCGGAAGA CGTGAAGCCC 
AAAACCCTAG CCCTGGAGAA AAGCATTAAT CATCAGATTG AGTCTCCGGG GGAAAGGCGG 
AAGAAAAGCC CTCGAGAGAA CTTCCGGGCT GGGCCCCTGT CCCCCTGCTC TCCTACCCCT 
CCTGGCCAGT CTCCAAACAG GTCTATAAGC GGGAAGAAGC TGTGCTCCTC CTGTGGGCTT 
GCTTTGGGGA AAGGCGCCGC AATGATCATC GAGACTCTGA ATCTCTACTT TCACATACAG 
TGTTTCAGGT GCGGCATTTG TAAAGGACAG CTAGGAGATG CAGTGAGTGG GACAGATGTC 
AGGATTCGCA ACGGTCTTCT AAACTGTACC GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCTACAA CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration.
Molecular biology of the cell28(8)1054-1065(2017 Apr)
Lin YH,Zhen YY,Chien KY,Lee IC,Lin WC,Chen MY,Pai LM

Expression and localization of an agmatinase-like protein in the rat brain.
Histochemistry and cell biology134(2)137-44(2010 Aug)
Mella C,Mart?nez F,de Los Angeles Garc?a M,Nualart F,Castro V,Bustos P,Carvajal N,Uribe E

Cloning and functional expression of a rodent brain cDNA encoding a novel protein with agmatinase activity, but not belonging to the arginase family.
Archives of biochemistry and biophysics461(1)146-50(2007 May)
Uribe E,Salas M,Enr?quez S,Orellana MS,Carvajal N

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Limch1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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