LIMCH1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMCH1
Entrez Gene ID	535165
Full Name	LIM and calponin homology domains 1
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015471594.1	XP_015327080.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_015471586.1	XP_015327072.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_015471587.1	XP_015327073.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_015471589.1	XP_015327075.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_015471591.1	XP_015327077.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_024993554.1	XP_024849322.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_024993556.1	XP_024849324.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_005207880.4	XP_005207937.2	LIM and calponin homology domains-containing protein 1 isoform X19
XM_015471588.1	XP_015327074.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_015471593.1	XP_015327079.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_015471596.1	XP_015327082.1	LIM and calponin homology domains-containing protein 1 isoform X11
XM_010806074.2	XP_010804376.1	LIM and calponin homology domains-containing protein 1 isoform X15
XM_015471597.2	XP_015327083.2	LIM and calponin homology domains-containing protein 1 isoform X9
XM_015471598.2	XP_015327084.2	LIM and calponin homology domains-containing protein 1 isoform X10
XM_010806074.3	XP_010804376.2	LIM and calponin homology domains-containing protein 1 isoform X17
XM_015471590.1	XP_015327076.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_015471595.1	XP_015327081.1	LIM and calponin homology domains-containing protein 1 isoform X10
XM_015471586.2	XP_015327072.2	LIM and calponin homology domains-containing protein 1 isoform X1
XM_015471590.2	XP_015327076.2	LIM and calponin homology domains-containing protein 1 isoform X2
XM_024993559.1	XP_024849327.1	LIM and calponin homology domains-containing protein 1 isoform X12
XM_024993561.1	XP_024849329.1	LIM and calponin homology domains-containing protein 1 isoform X15
XM_024993565.1	XP_024849333.1	LIM and calponin homology domains-containing protein 1 isoform X21
XM_024993566.1	XP_024849334.1	LIM and calponin homology domains-containing protein 1 isoform X22
XM_005207881.4	XP_005207938.2	LIM and calponin homology domains-containing protein 1 isoform X25
XM_005207881.3	XP_005207938.1	LIM and calponin homology domains-containing protein 1 isoform X17
XM_015471597.1	XP_015327083.1	LIM and calponin homology domains-containing protein 1 isoform X12
XM_005207880.3	XP_005207937.1	LIM and calponin homology domains-containing protein 1 isoform X16
XM_015471591.2	XP_015327077.2	LIM and calponin homology domains-containing protein 1 isoform X3
XM_024993555.1	XP_024849323.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_024993558.1	XP_024849326.1	LIM and calponin homology domains-containing protein 1 isoform X11
XM_005207879.4	XP_005207936.2	LIM and calponin homology domains-containing protein 1 isoform X13
XM_024993562.1	XP_024849330.1	LIM and calponin homology domains-containing protein 1 isoform X16
XM_015471598.1	XP_015327084.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_005207879.3	XP_005207936.1	LIM and calponin homology domains-containing protein 1 isoform X14
XM_005207882.3	XP_005207939.1	LIM and calponin homology domains-containing protein 1 isoform X18
XM_015471592.1	XP_015327078.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_024993553.1	XP_024849321.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_024993557.1	XP_024849325.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_024993560.1	XP_024849328.1	LIM and calponin homology domains-containing protein 1 isoform X14
XM_024993563.1	XP_024849331.1	LIM and calponin homology domains-containing protein 1 isoform X18
XM_024993564.1	XP_024849332.1	LIM and calponin homology domains-containing protein 1 isoform X20
XM_024993567.1	XP_024849335.1	LIM and calponin homology domains-containing protein 1 isoform X23
XM_024993568.1	XP_024849336.1	LIM and calponin homology domains-containing protein 1 isoform X24
XM_005207882.4	XP_005207939.2	LIM and calponin homology domains-containing protein 1 isoform X26

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1
Homo sapiens (human)	LIMCH1	NP_055803.2

Gene Ontology

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa20365	XM_005207881.3 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa20366	XM_005207882.3 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa235552	XM_024993565.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235553	XM_024993566.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235554	XM_024993567.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235555	XM_024993568.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235556	XM_005207881.4 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235557	XM_005207882.4 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235540	XM_015471597.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235541	XM_015471598.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235542	XM_024993558.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235543	XM_024993559.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235544	XM_005207879.4 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235545	XM_024993560.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235546	XM_024993561.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235547	XM_024993562.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235548	XM_010806074.3 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235549	XM_024993563.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235550	XM_005207880.4 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235551	XM_024993564.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa90907	XM_015471595.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa90908	XM_015471596.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa90909	XM_015471597.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa90910	XM_015471598.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa20363	XM_005207879.3 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa20364	XM_005207880.3 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa53048	XM_010806074.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa235532	XM_015471586.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235533	XM_015471590.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235534	XM_015471591.2 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235535	XM_024993553.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235536	XM_024993554.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235537	XM_024993555.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235538	XM_024993556.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa235539	XM_024993557.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa90898	XM_015471586.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90899	XM_015471587.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90900	XM_015471588.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90901	XM_015471589.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90902	XM_015471590.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90903	XM_015471591.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90904	XM_015471592.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90905	XM_015471593.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90906	XM_015471594.1 Latest version!	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMCH1 ( XM_005207881.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207938.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

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CloneID	OBa20365
Clone ID Related Accession (Same CDS sequence)	XM_005207881.3
Accession Version	XM_005207881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207938.1
CDS	155..2863
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207881.3
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207881.3

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207882.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207939.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa20366
Clone ID Related Accession (Same CDS sequence)	XM_005207882.3
Accession Version	XM_005207882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAG GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207939.1
CDS	155..2827
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207882.3
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207882.3

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAG GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993565.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849333.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa235552
Clone ID Related Accession (Same CDS sequence)	XM_024993565.1
Accession Version	XM_024993565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849333.1
CDS	162..2957
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993565.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993565.1

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993566.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849334.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa235553
Clone ID Related Accession (Same CDS sequence)	XM_024993566.1
Accession Version	XM_024993566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TCTATAAGTG GGAAGAAGCT GTGCTCTTCG 
TGTGGGCTTC CACTGGGTAA AGGTGCTGCG ATGATCATTG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GCTTTAAGTG TGGGATTTGT AAAGGTCAGC TTGGAGACGC CGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTTCTA AACTGTAATG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCCACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849334.1
CDS	161..2947
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993566.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993566.1

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TCTATAAGTG GGAAGAAGCT GTGCTCTTCG 
TGTGGGCTTC CACTGGGTAA AGGTGCTGCG ATGATCATTG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GCTTTAAGTG TGGGATTTGT AAAGGTCAGC TTGGAGACGC CGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTTCTA AACTGTAATG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCCACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993567.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849335.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OBa235554
Clone ID Related Accession (Same CDS sequence)	XM_024993567.1
Accession Version	XM_024993567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGAAAA GCCCTCGAGA GAACTTCCAG 
GCTGGGCGCT TGTCCCCCTG CTCCCCCACC CCTCCTGGCC AGTCACCAAA CAGGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849335.1
CDS	162..2912
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993567.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993567.1

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGAAAA GCCCTCGAGA GAACTTCCAG 
GCTGGGCGCT TGTCCCCCTG CTCCCCCACC CCTCCTGGCC AGTCACCAAA CAGGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993568.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849336.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa235555
Clone ID Related Accession (Same CDS sequence)	XM_024993568.1
Accession Version	XM_024993568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTCAGTTT AAGTTCTGGG CCTGGGACCC AGAAGAGGAG 
CGCAGACGAC AGGAAAAATG GCAACAGGAG CAGGAGCGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAAGAA 
GAACGCAGAT ACTATGAGGA GGAGCGGAAA ATAATTGAGG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GCTCTGCAGA CCAGCTCTCT ACATCTTCCT CTGTGACAGA AGGCAGTGGG 
ACGGTGAATA AGATGGACTT GGAAAACTGT TGTGAAGAAG AACAAGAGAC AAGACAGAAG 
AGACCACTCC AGGAGGATAA CAGTGACTCA CTGCTGAAGA CTAGGGAAGG TGATCCACTG 
GAGGAGAAGG GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849336.1
CDS	160..2871
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993568.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993568.1

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTCAGTTT AAGTTCTGGG CCTGGGACCC AGAAGAGGAG 
CGCAGACGAC AGGAAAAATG GCAACAGGAG CAGGAGCGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAAGAA 
GAACGCAGAT ACTATGAGGA GGAGCGGAAA ATAATTGAGG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GCTCTGCAGA CCAGCTCTCT ACATCTTCCT CTGTGACAGA AGGCAGTGGG 
ACGGTGAATA AGATGGACTT GGAAAACTGT TGTGAAGAAG AACAAGAGAC AAGACAGAAG 
AGACCACTCC AGGAGGATAA CAGTGACTCA CTGCTGAAGA CTAGGGAAGG TGATCCACTG 
GAGGAGAAGG GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207881.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207938.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OBa235556
Clone ID Related Accession (Same CDS sequence)	XM_005207881.4
Accession Version	XM_005207881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207881.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207938.2
CDS	159..2867
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207881.4
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207881.4

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGATACGGA 
CCGAGAACTC CTGTGTCTGA CGACGCAGAG AGCACGAGTA TGTTTGACAT GCGGTGTGAG 
GAAGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA ACTGATAAAC 
AGCCAGCTGC GGGAAGAGGA TGACAAATGG CAGGATGACC TGGCTCGTTG GAAGAGCCGT 
AGAAGAAGTG CTTCTCAGGA CTTAATCAGA AAAGAGGAAG AAAGGAAAAA GATGGAGAAG 
CTGCTGGCTG GAGAGGATGG GACAAGGGAA CGAAGGAAAA GCATAAAAAC CTACAGAGAA 
ATCGTCCAAG AAAAAGAGCG GAGAGAAAGG GAGTTGCATG AAGCATACAA GAATGCACGG 
ACCCAGGAGG AGGCAGAGGG TATCCTTCAG CAGTACATCG AGAGATTCAC CATCAGTGAA 
GCTGTTCTTG AACGTTTGGA GATGCCAAAA ATCCTGGAAA GAAGCCATTC AACAGAGCCA 
AACTTATCCC CCTTCCTCAG CGACCCCAAC CCTATGAAAT ATCTGCGGCA ACAGTCACTG 
CCTCCACCCA AGTTCACTGC CACTGTGGAA ACCACCATCG CTCGTCCCAG CGTTCTGGAT 
GCCAGCATGT CAGCAGGCAG TGGGTCTCCG AGCAAAACTG TCTCTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGAGGTCCT GAAGACCTTC 
AAGGTCGATG GGAAAGTCAC CATGAATGGA GAGACGACTC ATGGTGATGA GGAGGAGAAG 
GAAAGAGAGT GTCCCACGGT GGCACTTGCC CCCTCGTTGA CCAAATCCCA GATGTTTGAA 
GGTGAGGCCA GAGTGCATGG GTCCCCCGTG GAGCTGAAAC AAGACAGCAA CAGCATTGAA 
ATCAACATAA AGAAGCCAAG TTCTCTTCCC CAAGAGCTGA CAGCCACCAC TGAAGAAACT 
GAAGCGAACA GTCGAGAAGA TGAAAATGAT GGTGAAGAAG CAGAAAAAGG GAATACAGAA 
GTTGCCCCAT CAGAGCCACA GCTTTTTACA ACAACTGTGA CCAGATCCAG CCCAACCGTG 
GCCTTTGTGG AGCTTTCACC CAGCCCTCAG CTGAAGAATG AGGTGCCAGA AGAGAAAGAG 
CAGAAGAAGC CAGAAAATGA AATGAGTGGG AAGGTGGAGC TGGTGCTGTC ACAAAAGGTG 
GTAAAGCCAA AATCTCCCGA GCCAGAGGCA ACCCTGACAT TTCCATTTCT AGACAAAATG 
CCTGAAACCA ACCAGTCACC TTTGCCAAAT CTCAATTCTC AAGTGGATTC TCCAAGTAGT 
GAGAAGTCAC CTGTAACTAC ACCTTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207882.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207939.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OBa235557
Clone ID Related Accession (Same CDS sequence)	XM_005207882.4
Accession Version	XM_005207882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207882.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207939.2
CDS	159..2831
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207882.4
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207882.4

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCGAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGGAGCACG 
AGTATGTTTG ACATGCGGTG TGAGGAAGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAACTGAT AAACAGCCAG CTGCGGGAAG AGGATGACAA ATGGCAGGAT 
GACCTGGCTC GTTGGAAGAG CCGTAGAAGA AGTGCTTCTC AGGACTTAAT CAGAAAAGAG 
GAAGAAAGGA AAAAGATGGA GAAGCTGCTG GCTGGAGAGG ATGGGACAAG GGAACGAAGG 
AAAAGCATAA AAACCTACAG AGAAATCGTC CAAGAAAAAG AGCGGAGAGA AAGGGAGTTG 
CATGAAGCAT ACAAGAATGC ACGGACCCAG GAGGAGGCAG AGGGTATCCT TCAGCAGTAC 
ATCGAGAGAT TCACCATCAG TGAAGCTGTT CTTGAACGTT TGGAGATGCC AAAAATCCTG 
GAAAGAAGCC ATTCAACAGA GCCAAACTTA TCCCCCTTCC TCAGCGACCC CAACCCTATG 
AAATATCTGC GGCAACAGTC ACTGCCTCCA CCCAAGTTCA CTGCCACTGT GGAAACCACC 
ATCGCTCGTC CCAGCGTTCT GGATGCCAGC ATGTCAGCAG GCAGTGGGTC TCCGAGCAAA 
ACTGTCTCTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAC 
TCTCAAGAGG TCCTGAAGAC CTTCAAGGTC GATGGGAAAG TCACCATGAA TGGAGAGACG 
ACTCATGGTG ATGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACT TGCCCCCTCG 
TTGACCAAAT CCCAGATGTT TGAAGGTGAG GCCAGAGTGC ATGGGTCCCC CGTGGAGCTG 
AAACAAGACA GCAACAGCAT TGAAATCAAC ATAAAGAAGC CAAGTTCTCT TCCCCAAGAG 
CTGACAGCCA CCACTGAAGA AACTGAAGCG AACAGTCGAG AAGATGAAAA TGATGGTGAA 
GAAGCAGAAA AAGGGAATAC AGAAGTTGCC CCATCAGAGC CACAGCTTTT TACAACAACT 
GTGACCAGAT CCAGCCCAAC CGTGGCCTTT GTGGAGCTTT CACCCAGCCC TCAGCTGAAG 
AATGAGGTGC CAGAAGAGAA AGAGCAGAAG AAGCCAGAAA ATGAAATGAG TGGGAAGGTG 
GAGCTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CCGAGCCAGA GGCAACCCTG 
ACATTTCCAT TTCTAGACAA AATGCCTGAA ACCAACCAGT CACCTTTGCC AAATCTCAAT 
TCTCAAGTGG ATTCTCCAAG TAGTGAGAAG TCACCTGTAA CTACACCTTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCCTAA CTCAAGAGGT CTTGACTCAT 
TCTGAGAACC CGGCATCAGA AGGAATCTAC CACCAGCTGG ATACAGAAGC TGGGGCCCCA 
CAGTGTGGGG TAAACCCACA GCTAGCTCAG GATCCATCCC AGAATCAGCA GGTATCAAAC 
CCACCAATGC ACATTTTAGA AGATGTGAAG CCAAAAACCC TTCCATTGGA CAAAAGCATT 
AACCATCAGA TTGAGTCTCC GAGTGAAAGG CGGAAGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471597.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327083.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa235540
Clone ID Related Accession (Same CDS sequence)	XM_015471597.2
Accession Version	XM_015471597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327083.2
CDS	94..3432
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471597.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471597.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAAGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471598.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327084.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa235541
Clone ID Related Accession (Same CDS sequence)	XM_015471598.2
Accession Version	XM_015471598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTCAGTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCAGCC TAACTCAAGA GGTCTTGACT 
CATTCTGAGA ACCCGGCATC AGAAGGAATC TACCACCAGC TGGATACAGA AGCTGGGGCC 
CCACAGTGTG GGGTAAACCC ACAGCTAGCT CAGGATCCAT CCCAGAATCA GCAGGTATCA 
AACCCACCAA TGCACATTTT AGAAGATGTG AAGCCAAAAA CCCTTCCATT GGACAAAAGC 
ATTAACCATC AGATTGAGTC TCCGAGTGAA AGGCGGAAGA AAAGCCCTCG AGAGAACTTC 
CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC ACCCCTCCTG GCCAGTCACC AAACAGGTAC 
AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC TCTTCGTGTG GGCTTCCACT GGGTAAAGGT 
GCTGCGATGA TCATTGAGAC CCTCAATCTC TATTTTCACA TCCAGTGCTT TAAGTGTGGG 
ATTTGTAAAG GTCAGCTTGG AGACGCCGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTTCTAAACT GTAATGACTG CTACATGCGA TCCAGAAGTG CTGGCCAGCC CACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327084.2
CDS	94..3396
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471598.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471598.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTCAGTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAA GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCAGCC TAACTCAAGA GGTCTTGACT 
CATTCTGAGA ACCCGGCATC AGAAGGAATC TACCACCAGC TGGATACAGA AGCTGGGGCC 
CCACAGTGTG GGGTAAACCC ACAGCTAGCT CAGGATCCAT CCCAGAATCA GCAGGTATCA 
AACCCACCAA TGCACATTTT AGAAGATGTG AAGCCAAAAA CCCTTCCATT GGACAAAAGC 
ATTAACCATC AGATTGAGTC TCCGAGTGAA AGGCGGAAGA AAAGCCCTCG AGAGAACTTC 
CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC ACCCCTCCTG GCCAGTCACC AAACAGGTAC 
AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC TCTTCGTGTG GGCTTCCACT GGGTAAAGGT 
GCTGCGATGA TCATTGAGAC CCTCAATCTC TATTTTCACA TCCAGTGCTT TAAGTGTGGG 
ATTTGTAAAG GTCAGCTTGG AGACGCCGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTTCTAAACT GTAATGACTG CTACATGCGA TCCAGAAGTG CTGGCCAGCC CACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993558.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849326.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa235542
Clone ID Related Accession (Same CDS sequence)	XM_024993558.1
Accession Version	XM_024993558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCAGC 
CTAACTCAAG AGGTCTTGAC TCATTCTGAG AACCCGGCAT CAGAAGGAAT CTACCACCAG 
CTGGATACAG AAGCTGGGGC CCCACAGTGT GGGGTAAACC CACAGCTAGC TCAGGATCCA 
TCCCAGAATC AGCAGGTATC AAACCCACCA ATGCACATTT TAGAAGATGT GAAGCCAAAA 
ACCCTTCCAT TGGACAAAAG CATTAACCAT CAGATTGAGT CTCCGAGTGA AAGGCGGAAG 
AAAAGCCCTC GAGAGAACTT CCAGGCTGGG CGCTTGTCCC CCTGCTCCCC CACCCCTCCT 
GGCCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT 
GGGCTTCCAC TGGGTAAAGG TGCTGCGATG ATCATTGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGCT TTAAGTGTGG GATTTGTAAA GGTCAGCTTG GAGACGCCGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTTCTAAAC TGTAATGACT GCTACATGCG ATCCAGAAGT 
GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849326.1
CDS	94..3357
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993558.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993558.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCAGC 
CTAACTCAAG AGGTCTTGAC TCATTCTGAG AACCCGGCAT CAGAAGGAAT CTACCACCAG 
CTGGATACAG AAGCTGGGGC CCCACAGTGT GGGGTAAACC CACAGCTAGC TCAGGATCCA 
TCCCAGAATC AGCAGGTATC AAACCCACCA ATGCACATTT TAGAAGATGT GAAGCCAAAA 
ACCCTTCCAT TGGACAAAAG CATTAACCAT CAGATTGAGT CTCCGAGTGA AAGGCGGAAG 
AAAAGCCCTC GAGAGAACTT CCAGGCTGGG CGCTTGTCCC CCTGCTCCCC CACCCCTCCT 
GGCCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT 
GGGCTTCCAC TGGGTAAAGG TGCTGCGATG ATCATTGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGCT TTAAGTGTGG GATTTGTAAA GGTCAGCTTG GAGACGCCGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTTCTAAAC TGTAATGACT GCTACATGCG ATCCAGAAGT 
GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993559.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849327.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa235543
Clone ID Related Accession (Same CDS sequence)	XM_024993559.1
Accession Version	XM_024993559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849327.1
CDS	94..3351
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993559.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993559.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207879.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207936.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa235544
Clone ID Related Accession (Same CDS sequence)	XM_005207879.4
Accession Version	XM_005207879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207936.2
CDS	94..3345
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207879.4
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207879.4

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993560.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849328.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

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CloneID	OBa235545
Clone ID Related Accession (Same CDS sequence)	XM_024993560.1
Accession Version	XM_024993560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAAGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849328.1
CDS	94..3342
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993560.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993560.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAAGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993561.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849329.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa235546
Clone ID Related Accession (Same CDS sequence)	XM_024993561.1
Accession Version	XM_024993561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849329.1
CDS	94..3342
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993561.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993561.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993562.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849330.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa235547
Clone ID Related Accession (Same CDS sequence)	XM_024993562.1
Accession Version	XM_024993562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGTAGTGAGA AGTCACCTGT AACTACACCT TTTAAGTTCT GGGCCTGGGA CCCAGAAGAG 
GAGCGCAGAC GACAGGAAAA ATGGCAACAG GAGCAGGAGC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAA 
GAAGAACGCA GATACTATGA GGAGGAGCGG AAAATAATTG AGGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGCTCTGC AGACCAGCTC TCTACATCTT CCTCTGTGAC AGAAGGCAGT 
GGGACGGTGA ATAAGATGGA CTTGGAAAAC TGTTGTGAAG AAGAACAAGA GACAAGACAG 
AAGAGACCAC TCCAGGAGGA TAACAGTGAC TCACTGCTGA AGACTAGGGA AGGTGATCCA 
CTGGAGGAGA AGGGCAGCCT AACTCAAGAG GTCTTGACTC ATTCTGAGAA CCCGGCATCA 
GAAGGAATCT ACCACCAGCT GGATACAGAA GCTGGGGCCC CACAGTGTGG GGTAAACCCA 
CAGCTAGCTC AGGATCCATC CCAGAATCAG CAGGTATCAA ACCCACCAAT GCACATTTTA 
GAAGATGTGA AGCCAAAAAC CCTTCCATTG GACAAAAGCA TTAACCATCA GATTGAGTCT 
CCGAGTGAAA GGCGGAAGAA AAGCCCTCGA GAGAACTTCC AGGCTGGGCG CTTGTCCCCC 
TGCTCCCCCA CCCCTCCTGG CCAGTCACCA AACAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849330.1
CDS	94..3306
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993562.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993562.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGTAGTGAGA AGTCACCTGT AACTACACCT TTTAAGTTCT GGGCCTGGGA CCCAGAAGAG 
GAGCGCAGAC GACAGGAAAA ATGGCAACAG GAGCAGGAGC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAA 
GAAGAACGCA GATACTATGA GGAGGAGCGG AAAATAATTG AGGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGCTCTGC AGACCAGCTC TCTACATCTT CCTCTGTGAC AGAAGGCAGT 
GGGACGGTGA ATAAGATGGA CTTGGAAAAC TGTTGTGAAG AAGAACAAGA GACAAGACAG 
AAGAGACCAC TCCAGGAGGA TAACAGTGAC TCACTGCTGA AGACTAGGGA AGGTGATCCA 
CTGGAGGAGA AGGGCAGCCT AACTCAAGAG GTCTTGACTC ATTCTGAGAA CCCGGCATCA 
GAAGGAATCT ACCACCAGCT GGATACAGAA GCTGGGGCCC CACAGTGTGG GGTAAACCCA 
CAGCTAGCTC AGGATCCATC CCAGAATCAG CAGGTATCAA ACCCACCAAT GCACATTTTA 
GAAGATGTGA AGCCAAAAAC CCTTCCATTG GACAAAAGCA TTAACCATCA GATTGAGTCT 
CCGAGTGAAA GGCGGAAGAA AAGCCCTCGA GAGAACTTCC AGGCTGGGCG CTTGTCCCCC 
TGCTCCCCCA CCCCTCCTGG CCAGTCACCA AACAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_010806074.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804376.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa235548
Clone ID Related Accession (Same CDS sequence)	XM_010806074.3
Accession Version	XM_010806074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTTTTAA GTTCTGGGCC 
TGGGACCCAG AAGAGGAGCG CAGACGACAG GAAAAATGGC AACAGGAGCA GGAGCGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAAGAAGA ACGCAGATAC TATGAGGAGG AGCGGAAAAT AATTGAGGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGC TCTGCAGACC AGCTCTCTAC ATCTTCCTCT 
GTGACAGAAG GCAGTGGGAC GGTGAATAAG ATGGACTTGG AAAACTGTTG TGAAGAAGAA 
CAAGAGACAA GACAGAAGAG ACCACTCCAG GAGGATAACA GTGACTCACT GCTGAAGACT 
AGGGAAGGTG ATCCACTGGA GGAGAAGGGC AGCCTAACTC AAGAGGTCTT GACTCATTCT 
GAGAACCCGG CATCAGAAGG AATCTACCAC CAGCTGGATA CAGAAGCTGG GGCCCCACAG 
TGTGGGGTAA ACCCACAGCT AGCTCAGGAT CCATCCCAGA ATCAGCAGGT ATCAAACCCA 
CCAATGCACA TTTTAGAAGA TGTGAAGCCA AAAACCCTTC CATTGGACAA AAGCATTAAC 
CATCAGATTG AGTCTCCGAG TGAAAGGCGG AAGTCTATAA GTGGGAAGAA GCTGTGCTCT 
TCGTGTGGGC TTCCACTGGG TAAAGGTGCT GCGATGATCA TTGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGCTTTAA GTGTGGGATT TGTAAAGGTC AGCTTGGAGA CGCCGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTT CTAAACTGTA ATGACTGCTA CATGCGATCC 
AGAAGTGCTG GCCAGCCCAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804376.2
CDS	94..3303
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_010806074.3
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806074.3

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTTTTAA GTTCTGGGCC 
TGGGACCCAG AAGAGGAGCG CAGACGACAG GAAAAATGGC AACAGGAGCA GGAGCGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAAGAAGA ACGCAGATAC TATGAGGAGG AGCGGAAAAT AATTGAGGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGC TCTGCAGACC AGCTCTCTAC ATCTTCCTCT 
GTGACAGAAG GCAGTGGGAC GGTGAATAAG ATGGACTTGG AAAACTGTTG TGAAGAAGAA 
CAAGAGACAA GACAGAAGAG ACCACTCCAG GAGGATAACA GTGACTCACT GCTGAAGACT 
AGGGAAGGTG ATCCACTGGA GGAGAAGGGC AGCCTAACTC AAGAGGTCTT GACTCATTCT 
GAGAACCCGG CATCAGAAGG AATCTACCAC CAGCTGGATA CAGAAGCTGG GGCCCCACAG 
TGTGGGGTAA ACCCACAGCT AGCTCAGGAT CCATCCCAGA ATCAGCAGGT ATCAAACCCA 
CCAATGCACA TTTTAGAAGA TGTGAAGCCA AAAACCCTTC CATTGGACAA AAGCATTAAC 
CATCAGATTG AGTCTCCGAG TGAAAGGCGG AAGTCTATAA GTGGGAAGAA GCTGTGCTCT 
TCGTGTGGGC TTCCACTGGG TAAAGGTGCT GCGATGATCA TTGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGCTTTAA GTGTGGGATT TGTAAAGGTC AGCTTGGAGA CGCCGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTT CTAAACTGTA ATGACTGCTA CATGCGATCC 
AGAAGTGCTG GCCAGCCCAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993563.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849331.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa235549
Clone ID Related Accession (Same CDS sequence)	XM_024993563.1
Accession Version	XM_024993563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849331.1
CDS	94..3267
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993563.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993563.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA AGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207880.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207937.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa235550
Clone ID Related Accession (Same CDS sequence)	XM_005207880.4
Accession Version	XM_005207880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207937.2
CDS	94..3264
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207880.4
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207880.4

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993564.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849332.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa235551
Clone ID Related Accession (Same CDS sequence)	XM_024993564.1
Accession Version	XM_024993564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGTAGTGAGA AGTCACCTGT AACTACACCT TTTAAGTTCT GGGCCTGGGA CCCAGAAGAG 
GAGCGCAGAC GACAGGAAAA ATGGCAACAG GAGCAGGAGC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAA 
GAAGAACGCA GATACTATGA GGAGGAGCGG AAAATAATTG AGGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGCTCTGC AGACCAGCTC TCTACATCTT CCTCTGTGAC AGAAGGCAGT 
GGGACGGTGA ATAAGATGGA CTTGGAAAAC TGTTGTGAAG AAGAACAAGA GACAAGACAG 
AAGAGACCAC TCCAGGAGGA TAACAGTGAC TCACTGCTGA AGACTAGGGA AGGTGATCCA 
CTGGAGGAGA AGGGCAGCCT AACTCAAGAG GTCTTGACTC ATTCTGAGAA CCCGGCATCA 
GAAGGAATCT ACCACCAGCT GGATACAGAA GCTGGGGCCC CACAGTGTGG GGTAAACCCA 
CAGCTAGCTC AGGATCCATC CCAGAATCAG CAGGTATCAA ACCCACCAAT GCACATTTTA 
GAAGATGTGA AGCCAAAAAC CCTTCCATTG GACAAAAGCA TTAACCATCA GATTGAGTCT 
CCGAGTGAAA GGCGGAAGTC TATAAGTGGG AAGAAGCTGT GCTCTTCGTG TGGGCTTCCA 
CTGGGTAAAG GTGCTGCGAT GATCATTGAG ACCCTCAATC TCTATTTTCA CATCCAGTGC 
TTTAAGTGTG GGATTTGTAA AGGTCAGCTT GGAGACGCCG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTTCTAAA CTGTAATGAC TGCTACATGC GATCCAGAAG TGCTGGCCAG 
CCCACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849332.1
CDS	94..3228
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993564.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993564.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGTAGTGAGA AGTCACCTGT AACTACACCT TTTAAGTTCT GGGCCTGGGA CCCAGAAGAG 
GAGCGCAGAC GACAGGAAAA ATGGCAACAG GAGCAGGAGC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAA 
GAAGAACGCA GATACTATGA GGAGGAGCGG AAAATAATTG AGGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGCTCTGC AGACCAGCTC TCTACATCTT CCTCTGTGAC AGAAGGCAGT 
GGGACGGTGA ATAAGATGGA CTTGGAAAAC TGTTGTGAAG AAGAACAAGA GACAAGACAG 
AAGAGACCAC TCCAGGAGGA TAACAGTGAC TCACTGCTGA AGACTAGGGA AGGTGATCCA 
CTGGAGGAGA AGGGCAGCCT AACTCAAGAG GTCTTGACTC ATTCTGAGAA CCCGGCATCA 
GAAGGAATCT ACCACCAGCT GGATACAGAA GCTGGGGCCC CACAGTGTGG GGTAAACCCA 
CAGCTAGCTC AGGATCCATC CCAGAATCAG CAGGTATCAA ACCCACCAAT GCACATTTTA 
GAAGATGTGA AGCCAAAAAC CCTTCCATTG GACAAAAGCA TTAACCATCA GATTGAGTCT 
CCGAGTGAAA GGCGGAAGTC TATAAGTGGG AAGAAGCTGT GCTCTTCGTG TGGGCTTCCA 
CTGGGTAAAG GTGCTGCGAT GATCATTGAG ACCCTCAATC TCTATTTTCA CATCCAGTGC 
TTTAAGTGTG GGATTTGTAA AGGTCAGCTT GGAGACGCCG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTTCTAAA CTGTAATGAC TGCTACATGC GATCCAGAAG TGCTGGCCAG 
CCCACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327081.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

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CloneID	OBa90907
Clone ID Related Accession (Same CDS sequence)	XM_015471595.1
Accession Version	XM_015471595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGCTATCCT 
GAAACAATAG AGGAAGAGGA AGGTGAAGTG GGGTCTCATT GTGAAGAGGA CCCCGTCAGC 
CAAATGGACA CCAGTGGGAA GCCTCCTGAT GGTGGGTCAG AACTACCCAC CAGTTGTGGG 
AAGGAGCAGC CTCCTCCAGA GGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAGAAA 
GCTGCTGCTG AAATGCAAAA GCGCAGAAGG CTGGAGCAAG CCGGAATCAA GGTCATGCCC 
GCAGCACAGC GCTTTGCCAG TCAAAAGCAA TTAAATGATG AGAAAGAAGC TGTTCGAAAT 
ATTATCCTTC GTAAAGAAAA CCCTTTCCTG GTCCACCAAC ACGGCAGAGA TTCAGAATCA 
GAAGATGAAG TTGCATGTCG ACTGCCTGAT CTTGAGAAAG ATGACTTTGC TGCCAGGAGA 
GCAAGGATGA ACCAGACAAA GCCAGTGGTG CCATTGAACC AACTCCTCTA TGGGCCTTAC 
CGAAAAAAAG AGGCAGAGAA ATCAGATGGC AGCAAACAAC ACTCAAAGGG AATCTCAGAA 
AAGAAAACTC TAGAACATAA AAGAAACCAA GGCCAGACAG AAGAGGTGAA GTCAAGGGTG 
ACCTGTATTG TGCGGGCCCA GGAAAGTGAA CCTGCGGAAG CGGGACTCAG GAAGGTGCCG 
GATCTGTACA AGGATGACCT GGCCCAGCGG AGAATTCAGA GCAGGCTCGG CCCTCACCGT 
GAGGCCCCAA GCTTTATCAC GGTTGCCAGT ATAACAGAAG CCGACCTGGA GACATGGGAG 
AGACTGAAAG TGTCAGAAAA GACGAGGGAT GGAGATGTGG AGCACACGTG TGCCCCTGAG 
CCTTCACCAG AAATTAAAGC GGAAACTGCC ATCCGGGATG ACTTTGCCAG CCGCAAGGCA 
AGGGCCTATA AGAAAGCTTC CAGCAGCCCT AGGCAAAAGT TCGTGCACTT TGGGCCAGTG 
ACAGAGATAG ATCAGCAGAC GTGGAAGCGG CTCAGCATTG GCAGGGCGGG GCCGAGGGCG 
GATGCAGAGG AAGGCATCAG TCACGACAGC AAGACTCACA CATGTGCTGT GTCTGCTGCC 
TCAGCGGCCA CGTCCAGCTT AAAGGAAGAC CGCATGCTTA ATCCACAGAA TGACTGCAGA 
ACAGTGACTT CTGATGTAGA TGACTGTTGT AAAAGGGTCT CTCAGCTGGC TCCCGTGCCG 
GAGTCCCAGG AGGAGCGTAG CTTCAGTGCG GGCGAGGGCC CCAGGAGGAC AGAGGAAGTT 
ACACGCAAAC AGCAGCCACA GGATCGCAAA GAAGAGCCTG AAGGAGTTGC TCAAAGAGAT 
TCTGAGATGG CGCCAAAGGC CGAAAGATCC GAGGAGGGCG GCCAGCCACT ATACGGACCG 
AGAACTCCTG TGTCTGACGA CGCAGAGAGC ACGAGTATGT TTGACATGCG GTGTGAGGAA 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAACT GATAAACAGC 
CAGCTGCGGG AAGAGGATGA CAAATGGCAG GATGACCTGG CTCGTTGGAA GAGCCGTAGA 
AGAAGTGCTT CTCAGGACTT AATCAGAAAA GAGGAAGAAA GGAAAAAGAT GGAGAAGCTG 
CTGGCTGGAG AGGATGGGAC AAGGGAACGA AGGAAAAGCA TAAAAACCTA CAGAGAAATC 
GTCCAAGAAA AAGAGCGGAG AGAAAGGGAG TTGCATGAAG CATACAAGAA TGCACGGACC 
CAGGAGGAGG CAGAGGGTAT CCTTCAGCAG TACATCGAGA GATTCACCAT CAGTGAAGCT 
GTTCTTGAAC GTTTGGAGAT GCCAAAAATC CTGGAAAGAA GCCATTCAAC AGAGCCAAAC 
TTATCCCCCT TCCTCAGCGA CCCCAACCCT ATGAAATATC TGCGGCAACA GTCACTGCCT 
CCACCCAAGT TCACTGCCAC TGTGGAAACC ACCATCGCTC GTCCCAGCGT TCTGGATGCC 
AGCATGTCAG CAGGCAGTGG GTCTCCGAGC AAAACTGTCT CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AACTCTCAAG AGGTCCTGAA GACCTTCAAG 
GTCGATGGGA AAGTCACCAT GAATGGAGAG ACGACTCATG GTGATGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACTTGCCCCC TCGTTGACCA AATCCCAGAT GTTTGAAGGT 
GAGGCCAGAG TGCATGGGTC CCCCGTGGAG CTGAAACAAG ACAGCAACAG CATTGAAATC 
AACATAAAGA AGCCAAGTTC TCTTCCCCAA GAGCTGACAG CCACCACTGA AGAAACTGAA 
GCGAACAGTC GAGAAGATGA AAATGATGGT GAAGAAGCAG AAAAAGGGAA TACAGAAGTT 
GCCCCATCAG AGCCACAGCT TTTTACAACA ACTGTGACCA GATCCAGCCC AACCGTGGCC 
TTTGTGGAGC TTTCACCCAG CCCTCAGCTG AAGAATGAGG TGCCAGAAGA GAAAGAGCAG 
AAGAAGCCAG AAAATGAAAT GAGTGGGAAG GTGGAGCTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCCGAGCC AGAGGCAACC CTGACATTTC CATTTCTAGA CAAAATGCCT 
GAAACCAACC AGTCACCTTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGC 
ATTCCGCTGA GAGTTCAGAA CTCTTGGCGA CGTTCCCAGT TTTTCTCCCA GTCAGTGGAT 
TCTCCAAGTA GTGAGAAGTC ACCTGTAACT ACACCTCAGT TTAAGTTCTG GGCCTGGGAC 
CCAGAAGAGG AGCGCAGACG ACAGGAAAAA TGGCAACAGG AGCAGGAGCG TTTGCTCCAG 
GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG 
GTGGAAGAAG AAGAACGCAG ATACTATGAG GAGGAGCGGA AAATAATTGA GGACACTGTG 
GTTCCATTTA CTGTTTCTTC AAGCTCTGCA GACCAGCTCT CTACATCTTC CTCTGTGACA 
GAAGGCAGTG GGACGGTGAA TAAGATGGAC TTGGAAAACT GTTGTGAAGA AGAACAAGAG 
ACAAGACAGA GGAGACCACT CCAGGAGGAT AACAGTGACT CACTGCTGAA GACTAGGGAA 
GGTGATCCAC TGGAGGAGAA GGGCAGCAGC CTAACTCAAG AGGTCTTGAC TCATTCTGAG 
AACCCGGCAT CAGAAGGAAT CTACCACCAG CTGGATACAG AAGCTGGGGC CCCACAGTGT 
GGGGTAAACC CACAGCTAGC TCAGGATCCA TCCCAGAATC AGCAGGTATC AAACCCACCA 
ATGCACATTT TAGAAGATGT GAAGCCAAAA ACCCTTCCAT TGGACAAAAG CATTAACCAT 
CAGATTGAGT CTCCGAGTGA AAGGCGGAAG AAAAGCCCTC GAGAGAACTT CCAGGCTGGG 
CGCTTGTCCC CCTGCTCCCC CACCCCTCCT GGCCAGTCAC CAAACAGGTA CAAGAGGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327081.1
CDS	165..4418
Translation	MDPERRVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471595.1

ORF Insert Sequence:

ATGGATCCCG AAAGACGGGT CAAGGAGACT GATGACATTG AGAGCCCTAA ACGTAGTATT 
CGAGACAGTG GCTACATCGA CTGCTGGGAC TCCGAGCGCA GCGACTCCCT CTCTCCCCCT 
CGACACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCCCG TTCCCGGCAG 
ACTCCTTCGC CAGATGTAGT CCTCAGGGGA AGCAGTGATG GGAGGGGGAG CGACTCTGAA 
TCCGACCTGC CCCATCGGAA GCTGCCTGAT GTGAAGAAGG ATGACATGTC TGCAAGGCGG 
ACGTCCCACG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC CAACAAGAGC 
AACCAGACAG CCTACGTCCC CGCACCTCTG AGAAAGAAGA AGGCGGAGCG AGAGGAATAT 
CGGAAGAGCT GGAGTACCGC CACCTCCCCC CTGGGTGGGG AGAGGCCCTT CAGCTATCCT 
GAAACAATAG AGGAAGAGGA AGGTGAAGTG GGGTCTCATT GTGAAGAGGA CCCCGTCAGC 
CAAATGGACA CCAGTGGGAA GCCTCCTGAT GGTGGGTCAG AACTACCCAC CAGTTGTGGG 
AAGGAGCAGC CTCCTCCAGA GGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAGAAA 
GCTGCTGCTG AAATGCAAAA GCGCAGAAGG CTGGAGCAAG CCGGAATCAA GGTCATGCCC 
GCAGCACAGC GCTTTGCCAG TCAAAAGCAA TTAAATGATG AGAAAGAAGC TGTTCGAAAT 
ATTATCCTTC GTAAAGAAAA CCCTTTCCTG GTCCACCAAC ACGGCAGAGA TTCAGAATCA 
GAAGATGAAG TTGCATGTCG ACTGCCTGAT CTTGAGAAAG ATGACTTTGC TGCCAGGAGA 
GCAAGGATGA ACCAGACAAA GCCAGTGGTG CCATTGAACC AACTCCTCTA TGGGCCTTAC 
CGAAAAAAAG AGGCAGAGAA ATCAGATGGC AGCAAACAAC ACTCAAAGGG AATCTCAGAA 
AAGAAAACTC TAGAACATAA AAGAAACCAA GGCCAGACAG AAGAGGTGAA GTCAAGGGTG 
ACCTGTATTG TGCGGGCCCA GGAAAGTGAA CCTGCGGAAG CGGGACTCAG GAAGGTGCCG 
GATCTGTACA AGGATGACCT GGCCCAGCGG AGAATTCAGA GCAGGCTCGG CCCTCACCGT 
GAGGCCCCAA GCTTTATCAC GGTTGCCAGT ATAACAGAAG CCGACCTGGA GACATGGGAG 
AGACTGAAAG TGTCAGAAAA GACGAGGGAT GGAGATGTGG AGCACACGTG TGCCCCTGAG 
CCTTCACCAG AAATTAAAGC GGAAACTGCC ATCCGGGATG ACTTTGCCAG CCGCAAGGCA 
AGGGCCTATA AGAAAGCTTC CAGCAGCCCT AGGCAAAAGT TCGTGCACTT TGGGCCAGTG 
ACAGAGATAG ATCAGCAGAC GTGGAAGCGG CTCAGCATTG GCAGGGCGGG GCCGAGGGCG 
GATGCAGAGG AAGGCATCAG TCACGACAGC AAGACTCACA CATGTGCTGT GTCTGCTGCC 
TCAGCGGCCA CGTCCAGCTT AAAGGAAGAC CGCATGCTTA ATCCACAGAA TGACTGCAGA 
ACAGTGACTT CTGATGTAGA TGACTGTTGT AAAAGGGTCT CTCAGCTGGC TCCCGTGCCG 
GAGTCCCAGG AGGAGCGTAG CTTCAGTGCG GGCGAGGGCC CCAGGAGGAC AGAGGAAGTT 
ACACGCAAAC AGCAGCCACA GGATCGCAAA GAAGAGCCTG AAGGAGTTGC TCAAAGAGAT 
TCTGAGATGG CGCCAAAGGC CGAAAGATCC GAGGAGGGCG GCCAGCCACT ATACGGACCG 
AGAACTCCTG TGTCTGACGA CGCAGAGAGC ACGAGTATGT TTGACATGCG GTGTGAGGAA 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAACT GATAAACAGC 
CAGCTGCGGG AAGAGGATGA CAAATGGCAG GATGACCTGG CTCGTTGGAA GAGCCGTAGA 
AGAAGTGCTT CTCAGGACTT AATCAGAAAA GAGGAAGAAA GGAAAAAGAT GGAGAAGCTG 
CTGGCTGGAG AGGATGGGAC AAGGGAACGA AGGAAAAGCA TAAAAACCTA CAGAGAAATC 
GTCCAAGAAA AAGAGCGGAG AGAAAGGGAG TTGCATGAAG CATACAAGAA TGCACGGACC 
CAGGAGGAGG CAGAGGGTAT CCTTCAGCAG TACATCGAGA GATTCACCAT CAGTGAAGCT 
GTTCTTGAAC GTTTGGAGAT GCCAAAAATC CTGGAAAGAA GCCATTCAAC AGAGCCAAAC 
TTATCCCCCT TCCTCAGCGA CCCCAACCCT ATGAAATATC TGCGGCAACA GTCACTGCCT 
CCACCCAAGT TCACTGCCAC TGTGGAAACC ACCATCGCTC GTCCCAGCGT TCTGGATGCC 
AGCATGTCAG CAGGCAGTGG GTCTCCGAGC AAAACTGTCT CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AACTCTCAAG AGGTCCTGAA GACCTTCAAG 
GTCGATGGGA AAGTCACCAT GAATGGAGAG ACGACTCATG GTGATGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACTTGCCCCC TCGTTGACCA AATCCCAGAT GTTTGAAGGT 
GAGGCCAGAG TGCATGGGTC CCCCGTGGAG CTGAAACAAG ACAGCAACAG CATTGAAATC 
AACATAAAGA AGCCAAGTTC TCTTCCCCAA GAGCTGACAG CCACCACTGA AGAAACTGAA 
GCGAACAGTC GAGAAGATGA AAATGATGGT GAAGAAGCAG AAAAAGGGAA TACAGAAGTT 
GCCCCATCAG AGCCACAGCT TTTTACAACA ACTGTGACCA GATCCAGCCC AACCGTGGCC 
TTTGTGGAGC TTTCACCCAG CCCTCAGCTG AAGAATGAGG TGCCAGAAGA GAAAGAGCAG 
AAGAAGCCAG AAAATGAAAT GAGTGGGAAG GTGGAGCTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCCGAGCC AGAGGCAACC CTGACATTTC CATTTCTAGA CAAAATGCCT 
GAAACCAACC AGTCACCTTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGC 
ATTCCGCTGA GAGTTCAGAA CTCTTGGCGA CGTTCCCAGT TTTTCTCCCA GTCAGTGGAT 
TCTCCAAGTA GTGAGAAGTC ACCTGTAACT ACACCTCAGT TTAAGTTCTG GGCCTGGGAC 
CCAGAAGAGG AGCGCAGACG ACAGGAAAAA TGGCAACAGG AGCAGGAGCG TTTGCTCCAG 
GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG 
GTGGAAGAAG AAGAACGCAG ATACTATGAG GAGGAGCGGA AAATAATTGA GGACACTGTG 
GTTCCATTTA CTGTTTCTTC AAGCTCTGCA GACCAGCTCT CTACATCTTC CTCTGTGACA 
GAAGGCAGTG GGACGGTGAA TAAGATGGAC TTGGAAAACT GTTGTGAAGA AGAACAAGAG 
ACAAGACAGA GGAGACCACT CCAGGAGGAT AACAGTGACT CACTGCTGAA GACTAGGGAA 
GGTGATCCAC TGGAGGAGAA GGGCAGCAGC CTAACTCAAG AGGTCTTGAC TCATTCTGAG 
AACCCGGCAT CAGAAGGAAT CTACCACCAG CTGGATACAG AAGCTGGGGC CCCACAGTGT 
GGGGTAAACC CACAGCTAGC TCAGGATCCA TCCCAGAATC AGCAGGTATC AAACCCACCA 
ATGCACATTT TAGAAGATGT GAAGCCAAAA ACCCTTCCAT TGGACAAAAG CATTAACCAT 
CAGATTGAGT CTCCGAGTGA AAGGCGGAAG AAAAGCCCTC GAGAGAACTT CCAGGCTGGG 
CGCTTGTCCC CCTGCTCCCC CACCCCTCCT GGCCAGTCAC CAAACAGGTA CAAGAGGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471596.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327082.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa90908
Clone ID Related Accession (Same CDS sequence)	XM_015471596.1
Accession Version	XM_015471596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AGACTGATGA CATTGAGAGC CCTAAACGTA GTATTCGAGA CAGTGGCTAC 
ATCGACTGCT GGGACTCCGA GCGCAGCGAC TCCCTCTCTC CCCCTCGACA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCCCGTTCCC GGCAGACTCC TTCGCCAGAT 
GTAGTCCTCA GGGGAAGCAG TGATGGGAGG GGGAGCGACT CTGAATCCGA CCTGCCCCAT 
CGGAAGCTGC CTGATGTGAA GAAGGATGAC ATGTCTGCAA GGCGGACGTC CCACGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCCAACA AGAGCAACCA GACAGCCTAC 
GTCCCCGCAC CTCTGAGAAA GAAGAAGGCG GAGCGAGAGG AATATCGGAA GAGCTGGAGT 
ACCGCCACCT CCCCCCTGGG TGGGGAGAGG CCCTTCAGCT ATCCTGAAAC AATAGAGGAA 
GAGGAAGGTG AAGTGGGGTC TCATTGTGAA GAGGACCCCG TCAGCCAAAT GGACACCAGT 
GGGAAGCCTC CTGATGGTGG GTCAGAACTA CCCACCAGTT GTGGGAAGGA GCAGCCTCCT 
CCAGAGGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AGAAAGCTGC TGCTGAAATG 
CAAAAGCGCA GAAGGCTGGA GCAAGCCGGA ATCAAGGTCA TGCCCGCAGC ACAGCGCTTT 
GCCAGTCAAA AGCAATTAAA TGATGAGAAA GAAGCTGTTC GAAATATTAT CCTTCGTAAA 
GAAAACCCTT TCCTGGTCCA CCAACACGGC AGAGATTCAG AATCAGAAGA TGAAGTTGCA 
TGTCGACTGC CTGATCTTGA GAAAGATGAC TTTGCTGCCA GGAGAGCAAG GATGAACCAG 
ACAAAGCCAG TGGTGCCATT GAACCAACTC CTCTATGGGC CTTACCGAAA AAAAGAGGCA 
GAGAAATCAG ATGGCAGCAA ACAACACTCA AAGGGAATCT CAGAAAAGAA AACTCTAGAA 
CATAAAAGAA ACCAAGGCCA GACAGAAGAG GTGAAGTCAA GGGTGACCTG TATTGTGCGG 
GCCCAGGAAA GTGAACCTGC GGAAGCGGGA CTCAGGAAGG TGCCGGATCT GTACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGAGCAGG CTCGGCCCTC ACCGTGAGGC CCCAAGCTTT 
ATCACGGTTG CCAGTATAAC AGAAGCCGAC CTGGAGACAT GGGAGAGACT GAAAGTGTCA 
GAAAAGACGA GGGATGGAGA TGTGGAGCAC ACGTGTGCCC CTGAGCCTTC ACCAGAAATT 
AAAGCGGAAA CTGCCATCCG GGATGACTTT GCCAGCCGCA AGGCAAGGGC CTATAAGAAA 
GCTTCCAGCA GCCCTAGGCA AAAGTTCGTG CACTTTGGGC CAGTGACAGA GATAGATCAG 
CAGACGTGGA AGCGGCTCAG CATTGGCAGG GCGGGGCCGA GGGCGGATGC AGAGGAAGGC 
ATCAGTCACG ACAGCAAGAC TCACACATGT GCTGTGTCTG CTGCCTCAGC GGCCACGTCC 
AGCTTAAAGG AAGACCGCAT GCTTAATCCA CAGAATGACT GCAGAACAGT GACTTCTGAT 
GTAGATGACT GTTGTAAAAG GGTCTCTCAG CTGGCTCCCG TGCCGGAGTC CCAGGAGGAG 
CGTAGCTTCA GTGCGGGCGA GGGCCCCAGG AGGACAGAGG AAGTTACACG CAAACAGCAG 
CCACAGGATC GCAAAGAAGA GCCTGAAGGA GTTGCTCAAA GAGATTCTGA GATGGCGCCA 
AAGGCCGAAA GATCCGAGGA GGGCGGCCAG CCACTATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327082.1
CDS	418..4656
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471596.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471596.1

ORF Insert Sequence:

ATGAGAAAGG AGACTGATGA CATTGAGAGC CCTAAACGTA GTATTCGAGA CAGTGGCTAC 
ATCGACTGCT GGGACTCCGA GCGCAGCGAC TCCCTCTCTC CCCCTCGACA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCCCGTTCCC GGCAGACTCC TTCGCCAGAT 
GTAGTCCTCA GGGGAAGCAG TGATGGGAGG GGGAGCGACT CTGAATCCGA CCTGCCCCAT 
CGGAAGCTGC CTGATGTGAA GAAGGATGAC ATGTCTGCAA GGCGGACGTC CCACGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCCAACA AGAGCAACCA GACAGCCTAC 
GTCCCCGCAC CTCTGAGAAA GAAGAAGGCG GAGCGAGAGG AATATCGGAA GAGCTGGAGT 
ACCGCCACCT CCCCCCTGGG TGGGGAGAGG CCCTTCAGCT ATCCTGAAAC AATAGAGGAA 
GAGGAAGGTG AAGTGGGGTC TCATTGTGAA GAGGACCCCG TCAGCCAAAT GGACACCAGT 
GGGAAGCCTC CTGATGGTGG GTCAGAACTA CCCACCAGTT GTGGGAAGGA GCAGCCTCCT 
CCAGAGGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AGAAAGCTGC TGCTGAAATG 
CAAAAGCGCA GAAGGCTGGA GCAAGCCGGA ATCAAGGTCA TGCCCGCAGC ACAGCGCTTT 
GCCAGTCAAA AGCAATTAAA TGATGAGAAA GAAGCTGTTC GAAATATTAT CCTTCGTAAA 
GAAAACCCTT TCCTGGTCCA CCAACACGGC AGAGATTCAG AATCAGAAGA TGAAGTTGCA 
TGTCGACTGC CTGATCTTGA GAAAGATGAC TTTGCTGCCA GGAGAGCAAG GATGAACCAG 
ACAAAGCCAG TGGTGCCATT GAACCAACTC CTCTATGGGC CTTACCGAAA AAAAGAGGCA 
GAGAAATCAG ATGGCAGCAA ACAACACTCA AAGGGAATCT CAGAAAAGAA AACTCTAGAA 
CATAAAAGAA ACCAAGGCCA GACAGAAGAG GTGAAGTCAA GGGTGACCTG TATTGTGCGG 
GCCCAGGAAA GTGAACCTGC GGAAGCGGGA CTCAGGAAGG TGCCGGATCT GTACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGAGCAGG CTCGGCCCTC ACCGTGAGGC CCCAAGCTTT 
ATCACGGTTG CCAGTATAAC AGAAGCCGAC CTGGAGACAT GGGAGAGACT GAAAGTGTCA 
GAAAAGACGA GGGATGGAGA TGTGGAGCAC ACGTGTGCCC CTGAGCCTTC ACCAGAAATT 
AAAGCGGAAA CTGCCATCCG GGATGACTTT GCCAGCCGCA AGGCAAGGGC CTATAAGAAA 
GCTTCCAGCA GCCCTAGGCA AAAGTTCGTG CACTTTGGGC CAGTGACAGA GATAGATCAG 
CAGACGTGGA AGCGGCTCAG CATTGGCAGG GCGGGGCCGA GGGCGGATGC AGAGGAAGGC 
ATCAGTCACG ACAGCAAGAC TCACACATGT GCTGTGTCTG CTGCCTCAGC GGCCACGTCC 
AGCTTAAAGG AAGACCGCAT GCTTAATCCA CAGAATGACT GCAGAACAGT GACTTCTGAT 
GTAGATGACT GTTGTAAAAG GGTCTCTCAG CTGGCTCCCG TGCCGGAGTC CCAGGAGGAG 
CGTAGCTTCA GTGCGGGCGA GGGCCCCAGG AGGACAGAGG AAGTTACACG CAAACAGCAG 
CCACAGGATC GCAAAGAAGA GCCTGAAGGA GTTGCTCAAA GAGATTCTGA GATGGCGCCA 
AAGGCCGAAA GATCCGAGGA GGGCGGCCAG CCACTATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471597.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327083.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa90909
Clone ID Related Accession (Same CDS sequence)	XM_015471597.1
Accession Version	XM_015471597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327083.1
CDS	72..3410
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471597.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471597.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGAGTCG CCTGGCACTG CCAGCATTCC GCTGAGAGTT 
CAGAACTCTT GGCGACGTTC CCAGTTTTTC TCCCAGTCAG TGGATTCTCC AAGTAGTGAG 
AAGTCACCTG TAACTACACC TCAGTTTAAG TTCTGGGCCT GGGACCCAGA AGAGGAGCGC 
AGACGACAGG AAAAATGGCA ACAGGAGCAG GAGCGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA 
CGCAGATACT ATGAGGAGGA GCGGAAAATA ATTGAGGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGCT CTGCAGACCA GCTCTCTACA TCTTCCTCTG TGACAGAAGG CAGTGGGACG 
GTGAATAAGA TGGACTTGGA AAACTGTTGT GAAGAAGAAC AAGAGACAAG ACAGAGGAGA 
CCACTCCAGG AGGATAACAG TGACTCACTG CTGAAGACTA GGGAAGGTGA TCCACTGGAG 
GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC TTGACTCATT CTGAGAACCC GGCATCAGAA 
GGAATCTACC ACCAGCTGGA TACAGAAGCT GGGGCCCCAC AGTGTGGGGT AAACCCACAG 
CTAGCTCAGG ATCCATCCCA GAATCAGCAG GTATCAAACC CACCAATGCA CATTTTAGAA 
GATGTGAAGC CAAAAACCCT TCCATTGGAC AAAAGCATTA ACCATCAGAT TGAGTCTCCG 
AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG AACTTCCAGG CTGGGCGCTT GTCCCCCTGC 
TCCCCCACCC CTCCTGGCCA GTCACCAAAC AGGTACAAGA GGTCTATAAG TGGGAAGAAG 
CTGTGCTCTT CGTGTGGGCT TCCACTGGGT AAAGGTGCTG CGATGATCAT TGAGACCCTC 
AATCTCTATT TTCACATCCA GTGCTTTAAG TGTGGGATTT GTAAAGGTCA GCTTGGAGAC 
GCCGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTTC TAAACTGTAA TGACTGCTAC 
ATGCGATCCA GAAGTGCTGG CCAGCCCACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471598.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327084.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa90910
Clone ID Related Accession (Same CDS sequence)	XM_015471598.1
Accession Version	XM_015471598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTCAGTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAG GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCAGCC TAACTCAAGA GGTCTTGACT 
CATTCTGAGA ACCCGGCATC AGAAGGAATC TACCACCAGC TGGATACAGA AGCTGGGGCC 
CCACAGTGTG GGGTAAACCC ACAGCTAGCT CAGGATCCAT CCCAGAATCA GCAGGTATCA 
AACCCACCAA TGCACATTTT AGAAGATGTG AAGCCAAAAA CCCTTCCATT GGACAAAAGC 
ATTAACCATC AGATTGAGTC TCCGAGTGAA AGGCGGAAGA AAAGCCCTCG AGAGAACTTC 
CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC ACCCCTCCTG GCCAGTCACC AAACAGGTAC 
AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC TCTTCGTGTG GGCTTCCACT GGGTAAAGGT 
GCTGCGATGA TCATTGAGAC CCTCAATCTC TATTTTCACA TCCAGTGCTT TAAGTGTGGG 
ATTTGTAAAG GTCAGCTTGG AGACGCCGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTTCTAAACT GTAATGACTG CTACATGCGA TCCAGAAGTG CTGGCCAGCC CACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327084.1
CDS	72..3374
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471598.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471598.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTCAGTT TAAGTTCTGG 
GCCTGGGACC CAGAAGAGGA GCGCAGACGA CAGGAAAAAT GGCAACAGGA GCAGGAGCGT 
TTGCTCCAGG AGAGATACCA GAAGGAGCAG GACAAGCTGA AAGAAGAGTG GGAAAAGGCC 
CAAAAGGAGG TGGAAGAAGA AGAACGCAGA TACTATGAGG AGGAGCGGAA AATAATTGAG 
GACACTGTGG TTCCATTTAC TGTTTCTTCA AGCTCTGCAG ACCAGCTCTC TACATCTTCC 
TCTGTGACAG AAGGCAGTGG GACGGTGAAT AAGATGGACT TGGAAAACTG TTGTGAAGAA 
GAACAAGAGA CAAGACAGAG GAGACCACTC CAGGAGGATA ACAGTGACTC ACTGCTGAAG 
ACTAGGGAAG GTGATCCACT GGAGGAGAAG GGCAGCAGCC TAACTCAAGA GGTCTTGACT 
CATTCTGAGA ACCCGGCATC AGAAGGAATC TACCACCAGC TGGATACAGA AGCTGGGGCC 
CCACAGTGTG GGGTAAACCC ACAGCTAGCT CAGGATCCAT CCCAGAATCA GCAGGTATCA 
AACCCACCAA TGCACATTTT AGAAGATGTG AAGCCAAAAA CCCTTCCATT GGACAAAAGC 
ATTAACCATC AGATTGAGTC TCCGAGTGAA AGGCGGAAGA AAAGCCCTCG AGAGAACTTC 
CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC ACCCCTCCTG GCCAGTCACC AAACAGGTAC 
AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC TCTTCGTGTG GGCTTCCACT GGGTAAAGGT 
GCTGCGATGA TCATTGAGAC CCTCAATCTC TATTTTCACA TCCAGTGCTT TAAGTGTGGG 
ATTTGTAAAG GTCAGCTTGG AGACGCCGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTTCTAAACT GTAATGACTG CTACATGCGA TCCAGAAGTG CTGGCCAGCC CACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207879.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207936.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa20363
Clone ID Related Accession (Same CDS sequence)	XM_005207879.3
Accession Version	XM_005207879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA GGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207936.1
CDS	72..3323
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207879.3
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207879.3

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTCAGT TTAAGTTCTG GGCCTGGGAC CCAGAAGAGG AGCGCAGACG ACAGGAAAAA 
TGGCAACAGG AGCAGGAGCG TTTGCTCCAG GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAAG AAGAACGCAG ATACTATGAG 
GAGGAGCGGA AAATAATTGA GGACACTGTG GTTCCATTTA CTGTTTCTTC AAGCTCTGCA 
GACCAGCTCT CTACATCTTC CTCTGTGACA GAAGGCAGTG GGACGGTGAA TAAGATGGAC 
TTGGAAAACT GTTGTGAAGA AGAACAAGAG ACAAGACAGA GGAGACCACT CCAGGAGGAT 
AACAGTGACT CACTGCTGAA GACTAGGGAA GGTGATCCAC TGGAGGAGAA GGGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005207880.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207937.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

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CloneID	OBa20364
Clone ID Related Accession (Same CDS sequence)	XM_005207880.3
Accession Version	XM_005207880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAGGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207937.1
CDS	72..3242
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005207880.3
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207880.3

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGATACG GACCGAGAAC TCCTGTGTCT 
GACGACGCAG AGAGCACGAG TATGTTTGAC ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAACTGATAA ACAGCCAGCT GCGGGAAGAG 
GATGACAAAT GGCAGGATGA CCTGGCTCGT TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG 
GACTTAATCA GAAAAGAGGA AGAAAGGAAA AAGATGGAGA AGCTGCTGGC TGGAGAGGAT 
GGGACAAGGG AACGAAGGAA AAGCATAAAA ACCTACAGAG AAATCGTCCA AGAAAAAGAG 
CGGAGAGAAA GGGAGTTGCA TGAAGCATAC AAGAATGCAC GGACCCAGGA GGAGGCAGAG 
GGTATCCTTC AGCAGTACAT CGAGAGATTC ACCATCAGTG AAGCTGTTCT TGAACGTTTG 
GAGATGCCAA AAATCCTGGA AAGAAGCCAT TCAACAGAGC CAAACTTATC CCCCTTCCTC 
AGCGACCCCA ACCCTATGAA ATATCTGCGG CAACAGTCAC TGCCTCCACC CAAGTTCACT 
GCCACTGTGG AAACCACCAT CGCTCGTCCC AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC 
AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAACTC TCAAGAGGTC CTGAAGACCT TCAAGGTCGA TGGGAAAGTC 
ACCATGAATG GAGAGACGAC TCATGGTGAT GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACTTG CCCCCTCGTT GACCAAATCC CAGATGTTTG AAGGTGAGGC CAGAGTGCAT 
GGGTCCCCCG TGGAGCTGAA ACAAGACAGC AACAGCATTG AAATCAACAT AAAGAAGCCA 
AGTTCTCTTC CCCAAGAGCT GACAGCCACC ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA 
GATGAAAATG ATGGTGAAGA AGCAGAAAAA GGGAATACAG AAGTTGCCCC ATCAGAGCCA 
CAGCTTTTTA CAACAACTGT GACCAGATCC AGCCCAACCG TGGCCTTTGT GGAGCTTTCA 
CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT 
GAAATGAGTG GGAAGGTGGA GCTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCC 
GAGCCAGAGG CAACCCTGAC ATTTCCATTT CTAGACAAAA TGCCTGAAAC CAACCAGTCA 
CCTTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGTA GTGAGAAGTC ACCTGTAACT 
ACACCTTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAGGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_010806074.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804376.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa53048
Clone ID Related Accession (Same CDS sequence)	XM_010806074.2
Accession Version	XM_010806074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTTTTAA GTTCTGGGCC 
TGGGACCCAG AAGAGGAGCG CAGACGACAG GAAAAATGGC AACAGGAGCA GGAGCGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAAGAAGA ACGCAGATAC TATGAGGAGG AGCGGAAAAT AATTGAGGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGC TCTGCAGACC AGCTCTCTAC ATCTTCCTCT 
GTGACAGAAG GCAGTGGGAC GGTGAATAAG ATGGACTTGG AAAACTGTTG TGAAGAAGAA 
CAAGAGACAA GACAGAGGAG ACCACTCCAG GAGGATAACA GTGACTCACT GCTGAAGACT 
AGGGAAGGTG ATCCACTGGA GGAGAAGGGC AGCCTAACTC AAGAGGTCTT GACTCATTCT 
GAGAACCCGG CATCAGAAGG AATCTACCAC CAGCTGGATA CAGAAGCTGG GGCCCCACAG 
TGTGGGGTAA ACCCACAGCT AGCTCAGGAT CCATCCCAGA ATCAGCAGGT ATCAAACCCA 
CCAATGCACA TTTTAGAAGA TGTGAAGCCA AAAACCCTTC CATTGGACAA AAGCATTAAC 
CATCAGATTG AGTCTCCGAG TGAAAGGCGG AAGTCTATAA GTGGGAAGAA GCTGTGCTCT 
TCGTGTGGGC TTCCACTGGG TAAAGGTGCT GCGATGATCA TTGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGCTTTAA GTGTGGGATT TGTAAAGGTC AGCTTGGAGA CGCCGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTT CTAAACTGTA ATGACTGCTA CATGCGATCC 
AGAAGTGCTG GCCAGCCCAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804376.1
CDS	72..3281
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_010806074.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806074.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGGAGCA CGAGTATGTT TGACATGCGG 
TGTGAGGAAG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAACTG 
ATAAACAGCC AGCTGCGGGA AGAGGATGAC AAATGGCAGG ATGACCTGGC TCGTTGGAAG 
AGCCGTAGAA GAAGTGCTTC TCAGGACTTA ATCAGAAAAG AGGAAGAAAG GAAAAAGATG 
GAGAAGCTGC TGGCTGGAGA GGATGGGACA AGGGAACGAA GGAAAAGCAT AAAAACCTAC 
AGAGAAATCG TCCAAGAAAA AGAGCGGAGA GAAAGGGAGT TGCATGAAGC ATACAAGAAT 
GCACGGACCC AGGAGGAGGC AGAGGGTATC CTTCAGCAGT ACATCGAGAG ATTCACCATC 
AGTGAAGCTG TTCTTGAACG TTTGGAGATG CCAAAAATCC TGGAAAGAAG CCATTCAACA 
GAGCCAAACT TATCCCCCTT CCTCAGCGAC CCCAACCCTA TGAAATATCT GCGGCAACAG 
TCACTGCCTC CACCCAAGTT CACTGCCACT GTGGAAACCA CCATCGCTCG TCCCAGCGTT 
CTGGATGCCA GCATGTCAGC AGGCAGTGGG TCTCCGAGCA AAACTGTCTC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ACTCTCAAGA GGTCCTGAAG 
ACCTTCAAGG TCGATGGGAA AGTCACCATG AATGGAGAGA CGACTCATGG TGATGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CTTGCCCCCT CGTTGACCAA ATCCCAGATG 
TTTGAAGGTG AGGCCAGAGT GCATGGGTCC CCCGTGGAGC TGAAACAAGA CAGCAACAGC 
ATTGAAATCA ACATAAAGAA GCCAAGTTCT CTTCCCCAAG AGCTGACAGC CACCACTGAA 
GAAACTGAAG CGAACAGTCG AGAAGATGAA AATGATGGTG AAGAAGCAGA AAAAGGGAAT 
ACAGAAGTTG CCCCATCAGA GCCACAGCTT TTTACAACAA CTGTGACCAG ATCCAGCCCA 
ACCGTGGCCT TTGTGGAGCT TTCACCCAGC CCTCAGCTGA AGAATGAGGT GCCAGAAGAG 
AAAGAGCAGA AGAAGCCAGA AAATGAAATG AGTGGGAAGG TGGAGCTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCCGAGCCA GAGGCAACCC TGACATTTCC ATTTCTAGAC 
AAAATGCCTG AAACCAACCA GTCACCTTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGCA TTCCGCTGAG AGTTCAGAAC TCTTGGCGAC GTTCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGTAG TGAGAAGTCA CCTGTAACTA CACCTTTTAA GTTCTGGGCC 
TGGGACCCAG AAGAGGAGCG CAGACGACAG GAAAAATGGC AACAGGAGCA GGAGCGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAAGAAGA ACGCAGATAC TATGAGGAGG AGCGGAAAAT AATTGAGGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGC TCTGCAGACC AGCTCTCTAC ATCTTCCTCT 
GTGACAGAAG GCAGTGGGAC GGTGAATAAG ATGGACTTGG AAAACTGTTG TGAAGAAGAA 
CAAGAGACAA GACAGAGGAG ACCACTCCAG GAGGATAACA GTGACTCACT GCTGAAGACT 
AGGGAAGGTG ATCCACTGGA GGAGAAGGGC AGCCTAACTC AAGAGGTCTT GACTCATTCT 
GAGAACCCGG CATCAGAAGG AATCTACCAC CAGCTGGATA CAGAAGCTGG GGCCCCACAG 
TGTGGGGTAA ACCCACAGCT AGCTCAGGAT CCATCCCAGA ATCAGCAGGT ATCAAACCCA 
CCAATGCACA TTTTAGAAGA TGTGAAGCCA AAAACCCTTC CATTGGACAA AAGCATTAAC 
CATCAGATTG AGTCTCCGAG TGAAAGGCGG AAGTCTATAA GTGGGAAGAA GCTGTGCTCT 
TCGTGTGGGC TTCCACTGGG TAAAGGTGCT GCGATGATCA TTGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGCTTTAA GTGTGGGATT TGTAAAGGTC AGCTTGGAGA CGCCGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTT CTAAACTGTA ATGACTGCTA CATGCGATCC 
AGAAGTGCTG GCCAGCCCAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471586.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327072.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235532
Clone ID Related Accession (Same CDS sequence)	XM_015471586.2
Accession Version	XM_015471586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAAGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327072.2
CDS	94..4809
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471586.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471586.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAAGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471590.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327076.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OBa235533
Clone ID Related Accession (Same CDS sequence)	XM_015471590.2
Accession Version	XM_015471590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG 
CTTCCACTGG GTAAAGGTGC TGCGATGATC ATTGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT CAGCTTGGAG ACGCCGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT TCTAAACTGT AATGACTGCT ACATGCGATC CAGAAGTGCT 
GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327076.2
CDS	94..4734
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471590.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471590.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG 
CTTCCACTGG GTAAAGGTGC TGCGATGATC ATTGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT CAGCTTGGAG ACGCCGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT TCTAAACTGT AATGACTGCT ACATGCGATC CAGAAGTGCT 
GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471591.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327077.2 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

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CloneID	OBa235534
Clone ID Related Accession (Same CDS sequence)	XM_015471591.2
Accession Version	XM_015471591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327077.2
CDS	94..4731
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471591.2
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471591.2

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993553.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849321.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa235535
Clone ID Related Accession (Same CDS sequence)	XM_024993553.1
Accession Version	XM_024993553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849321.1
CDS	94..4725
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993553.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993553.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTCTAT AAGTGGGAAG AAGCTGTGCT CTTCGTGTGG GCTTCCACTG 
GGTAAAGGTG CTGCGATGAT CATTGAGACC CTCAATCTCT ATTTTCACAT CCAGTGCTTT 
AAGTGTGGGA TTTGTAAAGG TCAGCTTGGA GACGCCGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TTCTAAACTG TAATGACTGC TACATGCGAT CCAGAAGTGC TGGCCAGCCC 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993554.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849322.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa235536
Clone ID Related Accession (Same CDS sequence)	XM_024993554.1
Accession Version	XM_024993554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CCTAACTCAA 
GAGGTCTTGA CTCATTCTGA GAACCCGGCA TCAGAAGGAA TCTACCACCA GCTGGATACA 
GAAGCTGGGG CCCCACAGTG TGGGGTAAAC CCACAGCTAG CTCAGGATCC ATCCCAGAAT 
CAGCAGGTAT CAAACCCACC AATGCACATT TTAGAAGATG TGAAGCCAAA AACCCTTCCA 
TTGGACAAAA GCATTAACCA TCAGATTGAG TCTCCGAGTG AAAGGCGGAA GAAAAGCCCT 
CGAGAGAACT TCCAGGCTGG GCGCTTGTCC CCCTGCTCCC CCACCCCTCC TGGCCAGTCA 
CCAAACAGGT CTATAAGTGG GAAGAAGCTG TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA 
GGTGCTGCGA TGATCATTGA GACCCTCAAT CTCTATTTTC ACATCCAGTG CTTTAAGTGT 
GGGATTTGTA AAGGTCAGCT TGGAGACGCC GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTTCTAA ACTGTAATGA CTGCTACATG CGATCCAGAA GTGCTGGCCA GCCCACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849322.1
CDS	94..4719
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993554.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993554.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CCTAACTCAA 
GAGGTCTTGA CTCATTCTGA GAACCCGGCA TCAGAAGGAA TCTACCACCA GCTGGATACA 
GAAGCTGGGG CCCCACAGTG TGGGGTAAAC CCACAGCTAG CTCAGGATCC ATCCCAGAAT 
CAGCAGGTAT CAAACCCACC AATGCACATT TTAGAAGATG TGAAGCCAAA AACCCTTCCA 
TTGGACAAAA GCATTAACCA TCAGATTGAG TCTCCGAGTG AAAGGCGGAA GAAAAGCCCT 
CGAGAGAACT TCCAGGCTGG GCGCTTGTCC CCCTGCTCCC CCACCCCTCC TGGCCAGTCA 
CCAAACAGGT CTATAAGTGG GAAGAAGCTG TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA 
GGTGCTGCGA TGATCATTGA GACCCTCAAT CTCTATTTTC ACATCCAGTG CTTTAAGTGT 
GGGATTTGTA AAGGTCAGCT TGGAGACGCC GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTTCTAA ACTGTAATGA CTGCTACATG CGATCCAGAA GTGCTGGCCA GCCCACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993555.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849323.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OBa235537
Clone ID Related Accession (Same CDS sequence)	XM_024993555.1
Accession Version	XM_024993555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849323.1
CDS	94..4647
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993555.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993555.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAAGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGTCT 
ATAAGTGGGA AGAAGCTGTG CTCTTCGTGT GGGCTTCCAC TGGGTAAAGG TGCTGCGATG 
ATCATTGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGCT TTAAGTGTGG GATTTGTAAA 
GGTCAGCTTG GAGACGCCGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTTCTAAAC 
TGTAATGACT GCTACATGCG ATCCAGAAGT GCTGGCCAGC CCACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993556.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849324.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

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CloneID	OBa235538
Clone ID Related Accession (Same CDS sequence)	XM_024993556.1
Accession Version	XM_024993556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849324.1
CDS	94..4644
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993556.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993556.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGTCTATA 
AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG CTTCCACTGG GTAAAGGTGC TGCGATGATC 
ATTGAGACCC TCAATCTCTA TTTTCACATC CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT 
CAGCTTGGAG ACGCCGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT TCTAAACTGT 
AATGACTGCT ACATGCGATC CAGAAGTGCT GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024993557.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849325.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa235539
Clone ID Related Accession (Same CDS sequence)	XM_024993557.1
Accession Version	XM_024993557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CCTAACTCAA 
GAGGTCTTGA CTCATTCTGA GAACCCGGCA TCAGAAGGAA TCTACCACCA GCTGGATACA 
GAAGCTGGGG CCCCACAGTG TGGGGTAAAC CCACAGCTAG CTCAGGATCC ATCCCAGAAT 
CAGCAGGTAT CAAACCCACC AATGCACATT TTAGAAGATG TGAAGCCAAA AACCCTTCCA 
TTGGACAAAA GCATTAACCA TCAGATTGAG TCTCCGAGTG AAAGGCGGAA GTCTATAAGT 
GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT CCACTGGGTA AAGGTGCTGC GATGATCATT 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGCTTTAAGT GTGGGATTTG TAAAGGTCAG 
CTTGGAGACG CCGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTTCT AAACTGTAAT 
GACTGCTACA TGCGATCCAG AAGTGCTGGC CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849325.1
CDS	94..4641
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQKRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024993557.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993557.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAAGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CCTAACTCAA 
GAGGTCTTGA CTCATTCTGA GAACCCGGCA TCAGAAGGAA TCTACCACCA GCTGGATACA 
GAAGCTGGGG CCCCACAGTG TGGGGTAAAC CCACAGCTAG CTCAGGATCC ATCCCAGAAT 
CAGCAGGTAT CAAACCCACC AATGCACATT TTAGAAGATG TGAAGCCAAA AACCCTTCCA 
TTGGACAAAA GCATTAACCA TCAGATTGAG TCTCCGAGTG AAAGGCGGAA GTCTATAAGT 
GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT CCACTGGGTA AAGGTGCTGC GATGATCATT 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGCTTTAAGT GTGGGATTTG TAAAGGTCAG 
CTTGGAGACG CCGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTTCT AAACTGTAAT 
GACTGCTACA TGCGATCCAG AAGTGCTGGC CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471586.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327072.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa90898
Clone ID Related Accession (Same CDS sequence)	XM_015471586.1
Accession Version	XM_015471586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327072.1
CDS	72..4787
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471586.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471586.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG GAAGAAGCTG 
TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA GGTGCTGCGA TGATCATTGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG CTTTAAGTGT GGGATTTGTA AAGGTCAGCT TGGAGACGCC 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTTCTAA ACTGTAATGA CTGCTACATG 
CGATCCAGAA GTGCTGGCCA GCCCACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471587.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327073.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OBa90899
Clone ID Related Accession (Same CDS sequence)	XM_015471587.1
Accession Version	XM_015471587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTTT TAAGTTCTGG GCCTGGGACC CAGAAGAGGA GCGCAGACGA 
CAGGAAAAAT GGCAACAGGA GCAGGAGCGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAAGA AGAACGCAGA 
TACTATGAGG AGGAGCGGAA AATAATTGAG GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGCTCTGCAG ACCAGCTCTC TACATCTTCC TCTGTGACAG AAGGCAGTGG GACGGTGAAT 
AAGATGGACT TGGAAAACTG TTGTGAAGAA GAACAAGAGA CAAGACAGAG GAGACCACTC 
CAGGAGGATA ACAGTGACTC ACTGCTGAAG ACTAGGGAAG GTGATCCACT GGAGGAGAAG 
GGCAGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGA AAAGCCCTCG AGAGAACTTC CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC 
ACCCCTCCTG GCCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327073.1
CDS	72..4784
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471587.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTTT TAAGTTCTGG GCCTGGGACC CAGAAGAGGA GCGCAGACGA 
CAGGAAAAAT GGCAACAGGA GCAGGAGCGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAAGA AGAACGCAGA 
TACTATGAGG AGGAGCGGAA AATAATTGAG GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGCTCTGCAG ACCAGCTCTC TACATCTTCC TCTGTGACAG AAGGCAGTGG GACGGTGAAT 
AAGATGGACT TGGAAAACTG TTGTGAAGAA GAACAAGAGA CAAGACAGAG GAGACCACTC 
CAGGAGGATA ACAGTGACTC ACTGCTGAAG ACTAGGGAAG GTGATCCACT GGAGGAGAAG 
GGCAGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGA AAAGCCCTCG AGAGAACTTC CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC 
ACCCCTCCTG GCCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327074.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

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CloneID	OBa90900
Clone ID Related Accession (Same CDS sequence)	XM_015471588.1
Accession Version	XM_015471588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGA AAAGCCCTCG AGAGAACTTC CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC 
ACCCCTCCTG GCCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327074.1
CDS	72..4784
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471588.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGA AAAGCCCTCG AGAGAACTTC CAGGCTGGGC GCTTGTCCCC CTGCTCCCCC 
ACCCCTCCTG GCCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGGAA GAAGCTGTGC 
TCTTCGTGTG GGCTTCCACT GGGTAAAGGT GCTGCGATGA TCATTGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGCTT TAAGTGTGGG ATTTGTAAAG GTCAGCTTGG AGACGCCGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTTCTAAACT GTAATGACTG CTACATGCGA 
TCCAGAAGTG CTGGCCAGCC CACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471589.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327075.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

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CloneID	OBa90901
Clone ID Related Accession (Same CDS sequence)	XM_015471589.1
Accession Version	XM_015471589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TCTATAAGTG GGAAGAAGCT GTGCTCTTCG 
TGTGGGCTTC CACTGGGTAA AGGTGCTGCG ATGATCATTG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GCTTTAAGTG TGGGATTTGT AAAGGTCAGC TTGGAGACGC CGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTTCTA AACTGTAATG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCCACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327075.1
CDS	72..4778
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471589.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471589.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGAAAAGCCC TCGAGAGAAC TTCCAGGCTG GGCGCTTGTC CCCCTGCTCC 
CCCACCCCTC CTGGCCAGTC ACCAAACAGG TCTATAAGTG GGAAGAAGCT GTGCTCTTCG 
TGTGGGCTTC CACTGGGTAA AGGTGCTGCG ATGATCATTG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GCTTTAAGTG TGGGATTTGT AAAGGTCAGC TTGGAGACGC CGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTTCTA AACTGTAATG ACTGCTACAT GCGATCCAGA 
AGTGCTGGCC AGCCCACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471590.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327076.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OBa90902
Clone ID Related Accession (Same CDS sequence)	XM_015471590.1
Accession Version	XM_015471590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAGGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG 
CTTCCACTGG GTAAAGGTGC TGCGATGATC ATTGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT CAGCTTGGAG ACGCCGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT TCTAAACTGT AATGACTGCT ACATGCGATC CAGAAGTGCT 
GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327076.1
CDS	72..4712
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471590.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471590.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTCAGTTTA AGTTCTGGGC CTGGGACCCA GAAGAGGAGC GCAGACGACA GGAAAAATGG 
CAACAGGAGC AGGAGCGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAAGAAG AACGCAGATA CTATGAGGAG 
GAGCGGAAAA TAATTGAGGA CACTGTGGTT CCATTTACTG TTTCTTCAAG CTCTGCAGAC 
CAGCTCTCTA CATCTTCCTC TGTGACAGAA GGCAGTGGGA CGGTGAATAA GATGGACTTG 
GAAAACTGTT GTGAAGAAGA ACAAGAGACA AGACAGAGGA GACCACTCCA GGAGGATAAC 
AGTGACTCAC TGCTGAAGAC TAGGGAAGGT GATCCACTGG AGGAGAAGGG CAGCAGCCTA 
ACTCAAGAGG TCTTGACTCA TTCTGAGAAC CCGGCATCAG AAGGAATCTA CCACCAGCTG 
GATACAGAAG CTGGGGCCCC ACAGTGTGGG GTAAACCCAC AGCTAGCTCA GGATCCATCC 
CAGAATCAGC AGGTATCAAA CCCACCAATG CACATTTTAG AAGATGTGAA GCCAAAAACC 
CTTCCATTGG ACAAAAGCAT TAACCATCAG ATTGAGTCTC CGAGTGAAAG GCGGAAGAAA 
AGCCCTCGAG AGAACTTCCA GGCTGGGCGC TTGTCCCCCT GCTCCCCCAC CCCTCCTGGC 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGGAAGA AGCTGTGCTC TTCGTGTGGG 
CTTCCACTGG GTAAAGGTGC TGCGATGATC ATTGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGCTTTA AGTGTGGGAT TTGTAAAGGT CAGCTTGGAG ACGCCGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT TCTAAACTGT AATGACTGCT ACATGCGATC CAGAAGTGCT 
GGCCAGCCCA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471591.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327077.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa90903
Clone ID Related Accession (Same CDS sequence)	XM_015471591.1
Accession Version	XM_015471591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAGGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327077.1
CDS	72..4709
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471591.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGTAGTG AGAAGTCACC TGTAACTACA 
CCTTTTAAGT TCTGGGCCTG GGACCCAGAA GAGGAGCGCA GACGACAGGA AAAATGGCAA 
CAGGAGCAGG AGCGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAAGAAGAAC GCAGATACTA TGAGGAGGAG 
CGGAAAATAA TTGAGGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGCTC TGCAGACCAG 
CTCTCTACAT CTTCCTCTGT GACAGAAGGC AGTGGGACGG TGAATAAGAT GGACTTGGAA 
AACTGTTGTG AAGAAGAACA AGAGACAAGA CAGAGGAGAC CACTCCAGGA GGATAACAGT 
GACTCACTGC TGAAGACTAG GGAAGGTGAT CCACTGGAGG AGAAGGGCAG CAGCCTAACT 
CAAGAGGTCT TGACTCATTC TGAGAACCCG GCATCAGAAG GAATCTACCA CCAGCTGGAT 
ACAGAAGCTG GGGCCCCACA GTGTGGGGTA AACCCACAGC TAGCTCAGGA TCCATCCCAG 
AATCAGCAGG TATCAAACCC ACCAATGCAC ATTTTAGAAG ATGTGAAGCC AAAAACCCTT 
CCATTGGACA AAAGCATTAA CCATCAGATT GAGTCTCCGA GTGAAAGGCG GAAGAAAAGC 
CCTCGAGAGA ACTTCCAGGC TGGGCGCTTG TCCCCCTGCT CCCCCACCCC TCCTGGCCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGGAAGAAGC TGTGCTCTTC GTGTGGGCTT 
CCACTGGGTA AAGGTGCTGC GATGATCATT GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGCTTTAAGT GTGGGATTTG TAAAGGTCAG CTTGGAGACG CCGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTTCT AAACTGTAAT GACTGCTACA TGCGATCCAG AAGTGCTGGC 
CAGCCCACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471592.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327078.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa90904
Clone ID Related Accession (Same CDS sequence)	XM_015471592.1
Accession Version	XM_015471592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327078.1
CDS	72..4700
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471592.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTCA GTTTAAGTTC TGGGCCTGGG ACCCAGAAGA GGAGCGCAGA 
CGACAGGAAA AATGGCAACA GGAGCAGGAG CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA AGAAGAACGC 
AGATACTATG AGGAGGAGCG GAAAATAATT GAGGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGCTCTG CAGACCAGCT CTCTACATCT TCCTCTGTGA CAGAAGGCAG TGGGACGGTG 
AATAAGATGG ACTTGGAAAA CTGTTGTGAA GAAGAACAAG AGACAAGACA GAGGAGACCA 
CTCCAGGAGG ATAACAGTGA CTCACTGCTG AAGACTAGGG AAGGTGATCC ACTGGAGGAG 
AAGGGCAGCA GCCTAACTCA AGAGGTCTTG ACTCATTCTG AGAACCCGGC ATCAGAAGGA 
ATCTACCACC AGCTGGATAC AGAAGCTGGG GCCCCACAGT GTGGGGTAAA CCCACAGCTA 
GCTCAGGATC CATCCCAGAA TCAGCAGGTA TCAAACCCAC CAATGCACAT TTTAGAAGAT 
GTGAAGCCAA AAACCCTTCC ATTGGACAAA AGCATTAACC ATCAGATTGA GTCTCCGAGT 
GAAAGGCGGA AGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327079.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

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CloneID	OBa90905
Clone ID Related Accession (Same CDS sequence)	XM_015471593.1
Accession Version	XM_015471593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGAGATTCAG AATCAGAAGA TGAAGTTGCA TGTCGACTGC CTGATCTTGA GAAAGATGAC 
TTTGCTGCCA GGAGAGCAAG GATGAACCAG ACAAAGCCAG TGGTGCCATT GAACCAACTC 
CTCTATGGGC CTTACCGAAA AAAAGAGGCA GAGAAATCAG ATGGCAGCAA ACAACACTCA 
AAGGGAATCT CAGAAAAGAA AACTCTAGAA CATAAAAGAA ACCAAGGCCA GACAGAAGAG 
GTGAAGTCAA GGGTGACCTG TATTGTGCGG GCCCAGGAAA GTGAACCTGC GGAAGCGGGA 
CTCAGGAAGG TGCCGGATCT GTACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGAGCAGG 
CTCGGCCCTC ACCGTGAGGC CCCAAGCTTT ATCACGGTTG CCAGTATAAC AGAAGCCGAC 
CTGGAGACAT GGGAGAGACT GAAAGTGTCA GAAAAGACGA GGGATGGAGA TGTGGAGCAC 
ACGTGTGCCC CTGAGCCTTC ACCAGAAATT AAAGCGGAAA CTGCCATCCG GGATGACTTT 
GCCAGCCGCA AGGCAAGGGC CTATAAGAAA GCTTCCAGCA GCCCTAGGCA AAAGTTCGTG 
CACTTTGGGC CAGTGACAGA GATAGATCAG CAGACGTGGA AGCGGCTCAG CATTGGCAGG 
GCGGGGCCGA GGGCGGATGC AGAGGAAGGC ATCAGTCACG ACAGCAAGAC TCACACATGT 
GCTGTGTCTG CTGCCTCAGC GGCCACGTCC AGCTTAAAGG AAGACCGCAT GCTTAATCCA 
CAGAATGACT GCAGAACAGT GACTTCTGAT GTAGATGACT GTTGTAAAAG GGTCTCTCAG 
CTGGCTCCCG TGCCGGAGTC CCAGGAGGAG CGTAGCTTCA GTGCGGGCGA GGGCCCCAGG 
AGGACAGAGG AAGTTACACG CAAACAGCAG CCACAGGATC GCAAAGAAGA GCCTGAAGGA 
GTTGCTCAAA GAGATTCTGA GATGGCGCCA AAGGCCGAAA GATCCGAGGA GGGCGGCCAG 
CCACTATACG GACCGAGAAC TCCTGTGTCT GACGACGCAG AGAGCACGAG TATGTTTGAC 
ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAACTGATAA ACAGCCAGCT GCGGGAAGAG GATGACAAAT GGCAGGATGA CCTGGCTCGT 
TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG GACTTAATCA GAAAAGAGGA AGAAAGGAAA 
AAGATGGAGA AGCTGCTGGC TGGAGAGGAT GGGACAAGGG AACGAAGGAA AAGCATAAAA 
ACCTACAGAG AAATCGTCCA AGAAAAAGAG CGGAGAGAAA GGGAGTTGCA TGAAGCATAC 
AAGAATGCAC GGACCCAGGA GGAGGCAGAG GGTATCCTTC AGCAGTACAT CGAGAGATTC 
ACCATCAGTG AAGCTGTTCT TGAACGTTTG GAGATGCCAA AAATCCTGGA AAGAAGCCAT 
TCAACAGAGC CAAACTTATC CCCCTTCCTC AGCGACCCCA ACCCTATGAA ATATCTGCGG 
CAACAGTCAC TGCCTCCACC CAAGTTCACT GCCACTGTGG AAACCACCAT CGCTCGTCCC 
AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGAGGTC 
CTGAAGACCT TCAAGGTCGA TGGGAAAGTC ACCATGAATG GAGAGACGAC TCATGGTGAT 
GAGGAGGAGA AGGAAAGAGA GTGTCCCACG GTGGCACTTG CCCCCTCGTT GACCAAATCC 
CAGATGTTTG AAGGTGAGGC CAGAGTGCAT GGGTCCCCCG TGGAGCTGAA ACAAGACAGC 
AACAGCATTG AAATCAACAT AAAGAAGCCA AGTTCTCTTC CCCAAGAGCT GACAGCCACC 
ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA GATGAAAATG ATGGTGAAGA AGCAGAAAAA 
GGGAATACAG AAGTTGCCCC ATCAGAGCCA CAGCTTTTTA CAACAACTGT GACCAGATCC 
AGCCCAACCG TGGCCTTTGT GGAGCTTTCA CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA 
GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT GAAATGAGTG GGAAGGTGGA GCTGGTGCTG 
TCACAAAAGG TGGTAAAGCC AAAATCTCCC GAGCCAGAGG CAACCCTGAC ATTTCCATTT 
CTAGACAAAA TGCCTGAAAC CAACCAGTCA CCTTTGCCAA ATCTCAATTC TCAAGAGTCG 
CCTGGCACTG CCAGCATTCC GCTGAGAGTT CAGAACTCTT GGCGACGTTC CCAGTTTTTC 
TCCCAGTCAG TGGATTCTCC AAGTAGTGAG AAGTCACCTG TAACTACACC TCAGTTTAAG 
TTCTGGGCCT GGGACCCAGA AGAGGAGCGC AGACGACAGG AAAAATGGCA ACAGGAGCAG 
GAGCGTTTGC TCCAGGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA CGCAGATACT ATGAGGAGGA GCGGAAAATA 
ATTGAGGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGCT CTGCAGACCA GCTCTCTACA 
TCTTCCTCTG TGACAGAAGG CAGTGGGACG GTGAATAAGA TGGACTTGGA AAACTGTTGT 
GAAGAAGAAC AAGAGACAAG ACAGAGGAGA CCACTCCAGG AGGATAACAG TGACTCACTG 
CTGAAGACTA GGGAAGGTGA TCCACTGGAG GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC 
TTGACTCATT CTGAGAACCC GGCATCAGAA GGAATCTACC ACCAGCTGGA TACAGAAGCT 
GGGGCCCCAC AGTGTGGGGT AAACCCACAG CTAGCTCAGG ATCCATCCCA GAATCAGCAG 
GTATCAAACC CACCAATGCA CATTTTAGAA GATGTGAAGC CAAAAACCCT TCCATTGGAC 
AAAAGCATTA ACCATCAGAT TGAGTCTCCG AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG 
AACTTCCAGG CTGGGCGCTT GTCCCCCTGC TCCCCCACCC CTCCTGGCCA GTCACCAAAC 
AGGTACAAGA GGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327079.1
CDS	72..4700
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFARDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKKSPRENFQAGRLSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471593.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGAGATTCAG AATCAGAAGA TGAAGTTGCA TGTCGACTGC CTGATCTTGA GAAAGATGAC 
TTTGCTGCCA GGAGAGCAAG GATGAACCAG ACAAAGCCAG TGGTGCCATT GAACCAACTC 
CTCTATGGGC CTTACCGAAA AAAAGAGGCA GAGAAATCAG ATGGCAGCAA ACAACACTCA 
AAGGGAATCT CAGAAAAGAA AACTCTAGAA CATAAAAGAA ACCAAGGCCA GACAGAAGAG 
GTGAAGTCAA GGGTGACCTG TATTGTGCGG GCCCAGGAAA GTGAACCTGC GGAAGCGGGA 
CTCAGGAAGG TGCCGGATCT GTACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGAGCAGG 
CTCGGCCCTC ACCGTGAGGC CCCAAGCTTT ATCACGGTTG CCAGTATAAC AGAAGCCGAC 
CTGGAGACAT GGGAGAGACT GAAAGTGTCA GAAAAGACGA GGGATGGAGA TGTGGAGCAC 
ACGTGTGCCC CTGAGCCTTC ACCAGAAATT AAAGCGGAAA CTGCCATCCG GGATGACTTT 
GCCAGCCGCA AGGCAAGGGC CTATAAGAAA GCTTCCAGCA GCCCTAGGCA AAAGTTCGTG 
CACTTTGGGC CAGTGACAGA GATAGATCAG CAGACGTGGA AGCGGCTCAG CATTGGCAGG 
GCGGGGCCGA GGGCGGATGC AGAGGAAGGC ATCAGTCACG ACAGCAAGAC TCACACATGT 
GCTGTGTCTG CTGCCTCAGC GGCCACGTCC AGCTTAAAGG AAGACCGCAT GCTTAATCCA 
CAGAATGACT GCAGAACAGT GACTTCTGAT GTAGATGACT GTTGTAAAAG GGTCTCTCAG 
CTGGCTCCCG TGCCGGAGTC CCAGGAGGAG CGTAGCTTCA GTGCGGGCGA GGGCCCCAGG 
AGGACAGAGG AAGTTACACG CAAACAGCAG CCACAGGATC GCAAAGAAGA GCCTGAAGGA 
GTTGCTCAAA GAGATTCTGA GATGGCGCCA AAGGCCGAAA GATCCGAGGA GGGCGGCCAG 
CCACTATACG GACCGAGAAC TCCTGTGTCT GACGACGCAG AGAGCACGAG TATGTTTGAC 
ATGCGGTGTG AGGAAGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAACTGATAA ACAGCCAGCT GCGGGAAGAG GATGACAAAT GGCAGGATGA CCTGGCTCGT 
TGGAAGAGCC GTAGAAGAAG TGCTTCTCAG GACTTAATCA GAAAAGAGGA AGAAAGGAAA 
AAGATGGAGA AGCTGCTGGC TGGAGAGGAT GGGACAAGGG AACGAAGGAA AAGCATAAAA 
ACCTACAGAG AAATCGTCCA AGAAAAAGAG CGGAGAGAAA GGGAGTTGCA TGAAGCATAC 
AAGAATGCAC GGACCCAGGA GGAGGCAGAG GGTATCCTTC AGCAGTACAT CGAGAGATTC 
ACCATCAGTG AAGCTGTTCT TGAACGTTTG GAGATGCCAA AAATCCTGGA AAGAAGCCAT 
TCAACAGAGC CAAACTTATC CCCCTTCCTC AGCGACCCCA ACCCTATGAA ATATCTGCGG 
CAACAGTCAC TGCCTCCACC CAAGTTCACT GCCACTGTGG AAACCACCAT CGCTCGTCCC 
AGCGTTCTGG ATGCCAGCAT GTCAGCAGGC AGTGGGTCTC CGAGCAAAAC TGTCTCTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGAGGTC 
CTGAAGACCT TCAAGGTCGA TGGGAAAGTC ACCATGAATG GAGAGACGAC TCATGGTGAT 
GAGGAGGAGA AGGAAAGAGA GTGTCCCACG GTGGCACTTG CCCCCTCGTT GACCAAATCC 
CAGATGTTTG AAGGTGAGGC CAGAGTGCAT GGGTCCCCCG TGGAGCTGAA ACAAGACAGC 
AACAGCATTG AAATCAACAT AAAGAAGCCA AGTTCTCTTC CCCAAGAGCT GACAGCCACC 
ACTGAAGAAA CTGAAGCGAA CAGTCGAGAA GATGAAAATG ATGGTGAAGA AGCAGAAAAA 
GGGAATACAG AAGTTGCCCC ATCAGAGCCA CAGCTTTTTA CAACAACTGT GACCAGATCC 
AGCCCAACCG TGGCCTTTGT GGAGCTTTCA CCCAGCCCTC AGCTGAAGAA TGAGGTGCCA 
GAAGAGAAAG AGCAGAAGAA GCCAGAAAAT GAAATGAGTG GGAAGGTGGA GCTGGTGCTG 
TCACAAAAGG TGGTAAAGCC AAAATCTCCC GAGCCAGAGG CAACCCTGAC ATTTCCATTT 
CTAGACAAAA TGCCTGAAAC CAACCAGTCA CCTTTGCCAA ATCTCAATTC TCAAGAGTCG 
CCTGGCACTG CCAGCATTCC GCTGAGAGTT CAGAACTCTT GGCGACGTTC CCAGTTTTTC 
TCCCAGTCAG TGGATTCTCC AAGTAGTGAG AAGTCACCTG TAACTACACC TCAGTTTAAG 
TTCTGGGCCT GGGACCCAGA AGAGGAGCGC AGACGACAGG AAAAATGGCA ACAGGAGCAG 
GAGCGTTTGC TCCAGGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAAGAAGAA CGCAGATACT ATGAGGAGGA GCGGAAAATA 
ATTGAGGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGCT CTGCAGACCA GCTCTCTACA 
TCTTCCTCTG TGACAGAAGG CAGTGGGACG GTGAATAAGA TGGACTTGGA AAACTGTTGT 
GAAGAAGAAC AAGAGACAAG ACAGAGGAGA CCACTCCAGG AGGATAACAG TGACTCACTG 
CTGAAGACTA GGGAAGGTGA TCCACTGGAG GAGAAGGGCA GCAGCCTAAC TCAAGAGGTC 
TTGACTCATT CTGAGAACCC GGCATCAGAA GGAATCTACC ACCAGCTGGA TACAGAAGCT 
GGGGCCCCAC AGTGTGGGGT AAACCCACAG CTAGCTCAGG ATCCATCCCA GAATCAGCAG 
GTATCAAACC CACCAATGCA CATTTTAGAA GATGTGAAGC CAAAAACCCT TCCATTGGAC 
AAAAGCATTA ACCATCAGAT TGAGTCTCCG AGTGAAAGGC GGAAGAAAAG CCCTCGAGAG 
AACTTCCAGG CTGGGCGCTT GTCCCCCTGC TCCCCCACCC CTCCTGGCCA GTCACCAAAC 
AGGTACAAGA GGTCTATAAG TGGGAAGAAG CTGTGCTCTT CGTGTGGGCT TCCACTGGGT 
AAAGGTGCTG CGATGATCAT TGAGACCCTC AATCTCTATT TTCACATCCA GTGCTTTAAG 
TGTGGGATTT GTAAAGGTCA GCTTGGAGAC GCCGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTTC TAAACTGTAA TGACTGCTAC ATGCGATCCA GAAGTGCTGG CCAGCCCACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015471594.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327080.1 Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

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CloneID	OBa90906
Clone ID Related Accession (Same CDS sequence)	XM_015471594.1
Accession Version	XM_015471594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTTT TAAGTTCTGG GCCTGGGACC CAGAAGAGGA GCGCAGACGA 
CAGGAAAAAT GGCAACAGGA GCAGGAGCGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAAGA AGAACGCAGA 
TACTATGAGG AGGAGCGGAA AATAATTGAG GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGCTCTGCAG ACCAGCTCTC TACATCTTCC TCTGTGACAG AAGGCAGTGG GACGGTGAAT 
AAGATGGACT TGGAAAACTG TTGTGAAGAA GAACAAGAGA CAAGACAGAG GAGACCACTC 
CAGGAGGATA ACAGTGACTC ACTGCTGAAG ACTAGGGAAG GTGATCCACT GGAGGAGAAG 
GGCAGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGT CTATAAGTGG GAAGAAGCTG TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA 
GGTGCTGCGA TGATCATTGA GACCCTCAAT CTCTATTTTC ACATCCAGTG CTTTAAGTGT 
GGGATTTGTA AAGGTCAGCT TGGAGACGCC GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTTCTAA ACTGTAATGA CTGCTACATG CGATCCAGAA GTGCTGGCCA GCCCACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327080.1
CDS	72..4697
Translation	MACPVLGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVAVKSLDYSRKLKNVLVTIYWLGKAANSCASYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSYPETIEEEEGEVGSHCEEDPVSQMDTSGKPPDGGSELPTSCGKEQPPPEGAVVAPAPKSEEKAAAEMQKRRRLEQAGIKVMPAAQRFASQKQLNDEKEAVRNIILRKENPFLVHQHGRDSESEDEVACRLPDLEKDDFAARRARMNQTKPVVPLNQLLYGPYRKKEAEKSDGSKQHSKGISEKKTLEHKRNQGQTEEVKSRVTCIVRAQESEPAEAGLRKVPDLYKDDLAQRRIQSRLGPHREAPSFITVASITEADLETWERLKVSEKTRDGDVEHTCAPEPSPEIKAETAIRDDFASRKARAYKKASSSPRQKFVHFGPVTEIDQQTWKRLSIGRAGPRADAEEGISHDSKTHTCAVSAASAATSSLKEDRMLNPQNDCRTVTSDVDDCCKRVSQLAPVPESQEERSFSAGEGPRRTEEVTRKQQPQDRKEEPEGVAQRDSEMAPKAERSEEGGQPLYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINSQLREEDDKWQDDLARWKSRRRSASQDLIRKEEERKKMEKLLAGEDGTRERRKSIKTYREIVQEKERRERELHEAYKNARTQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSPFLSDPNPMKYLRQQSLPPPKFTATVETTIARPSVLDASMSAGSGSPSKTVSPKAVPMLTPKPYSQPKNSQEVLKTFKVDGKVTMNGETTHGDEEEKERECPTVALAPSLTKSQMFEGEARVHGSPVELKQDSNSIEINIKKPSSLPQELTATTEETEANSREDENDGEEAEKGNTEVAPSEPQLFTTTVTRSSPTVAFVELSPSPQLKNEVPEEKEQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPETNQSPLPNLNSQESPGTASIPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSVTEGSGTVNKMDLENCCEEEQETRQRRPLQEDNSDSLLKTREGDPLEEKGSSLTQEVLTHSENPASEGIYHQLDTEAGAPQCGVNPQLAQDPSQNQQVSNPPMHILEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFKCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_015471594.1
Organism	Bos taurus(cattle)
Definition	Bos taurus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471594.1

ORF Insert Sequence:

ATGGCTTGTC CCGTTCTCGG TCTGGAAGCC CTCCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG ATTAGAAAAT GGAATCCTTC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGATTGGAC 
AATATCATAT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAGCTTTTT 
GACCCGAGTG ACCTGCAGGA TACGTCCAAC AGAGTCGCAG TCAAGAGTCT TGACTATAGT 
AGGAAGCTGA AAAATGTACT AGTGACCATT TACTGGCTGG GCAAAGCGGC GAACAGCTGT 
GCCTCGTACA GTGGGACTAC GCTGAACCTG AAGGAGTTTG AAGGCTTGCT GGCTCAGATG 
AGAAAGGAGA CTGATGACAT TGAGAGCCCT AAACGTAGTA TTCGAGACAG TGGCTACATC 
GACTGCTGGG ACTCCGAGCG CAGCGACTCC CTCTCTCCCC CTCGACACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCC CGTTCCCGGC AGACTCCTTC GCCAGATGTA 
GTCCTCAGGG GAAGCAGTGA TGGGAGGGGG AGCGACTCTG AATCCGACCT GCCCCATCGG 
AAGCTGCCTG ATGTGAAGAA GGATGACATG TCTGCAAGGC GGACGTCCCA CGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCCAACAAGA GCAACCAGAC AGCCTACGTC 
CCCGCACCTC TGAGAAAGAA GAAGGCGGAG CGAGAGGAAT ATCGGAAGAG CTGGAGTACC 
GCCACCTCCC CCCTGGGTGG GGAGAGGCCC TTCAGCTATC CTGAAACAAT AGAGGAAGAG 
GAAGGTGAAG TGGGGTCTCA TTGTGAAGAG GACCCCGTCA GCCAAATGGA CACCAGTGGG 
AAGCCTCCTG ATGGTGGGTC AGAACTACCC ACCAGTTGTG GGAAGGAGCA GCCTCCTCCA 
GAGGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAGA AAGCTGCTGC TGAAATGCAA 
AAGCGCAGAA GGCTGGAGCA AGCCGGAATC AAGGTCATGC CCGCAGCACA GCGCTTTGCC 
AGTCAAAAGC AATTAAATGA TGAGAAAGAA GCTGTTCGAA ATATTATCCT TCGTAAAGAA 
AACCCTTTCC TGGTCCACCA ACACGGCAGA GATTCAGAAT CAGAAGATGA AGTTGCATGT 
CGACTGCCTG ATCTTGAGAA AGATGACTTT GCTGCCAGGA GAGCAAGGAT GAACCAGACA 
AAGCCAGTGG TGCCATTGAA CCAACTCCTC TATGGGCCTT ACCGAAAAAA AGAGGCAGAG 
AAATCAGATG GCAGCAAACA ACACTCAAAG GGAATCTCAG AAAAGAAAAC TCTAGAACAT 
AAAAGAAACC AAGGCCAGAC AGAAGAGGTG AAGTCAAGGG TGACCTGTAT TGTGCGGGCC 
CAGGAAAGTG AACCTGCGGA AGCGGGACTC AGGAAGGTGC CGGATCTGTA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GAGCAGGCTC GGCCCTCACC GTGAGGCCCC AAGCTTTATC 
ACGGTTGCCA GTATAACAGA AGCCGACCTG GAGACATGGG AGAGACTGAA AGTGTCAGAA 
AAGACGAGGG ATGGAGATGT GGAGCACACG TGTGCCCCTG AGCCTTCACC AGAAATTAAA 
GCGGAAACTG CCATCCGGGA TGACTTTGCC AGCCGCAAGG CAAGGGCCTA TAAGAAAGCT 
TCCAGCAGCC CTAGGCAAAA GTTCGTGCAC TTTGGGCCAG TGACAGAGAT AGATCAGCAG 
ACGTGGAAGC GGCTCAGCAT TGGCAGGGCG GGGCCGAGGG CGGATGCAGA GGAAGGCATC 
AGTCACGACA GCAAGACTCA CACATGTGCT GTGTCTGCTG CCTCAGCGGC CACGTCCAGC 
TTAAAGGAAG ACCGCATGCT TAATCCACAG AATGACTGCA GAACAGTGAC TTCTGATGTA 
GATGACTGTT GTAAAAGGGT CTCTCAGCTG GCTCCCGTGC CGGAGTCCCA GGAGGAGCGT 
AGCTTCAGTG CGGGCGAGGG CCCCAGGAGG ACAGAGGAAG TTACACGCAA ACAGCAGCCA 
CAGGATCGCA AAGAAGAGCC TGAAGGAGTT GCTCAAAGAG ATTCTGAGAT GGCGCCAAAG 
GCCGAAAGAT CCGAGGAGGG CGGCCAGCCA CTATACGGAC CGAGAACTCC TGTGTCTGAC 
GACGCAGAGA GCACGAGTAT GTTTGACATG CGGTGTGAGG AAGAGGCCGC GGTGCAGCCG 
CACAGCAGGG CCCGCCAGGA GCAGCTGCAA CTGATAAACA GCCAGCTGCG GGAAGAGGAT 
GACAAATGGC AGGATGACCT GGCTCGTTGG AAGAGCCGTA GAAGAAGTGC TTCTCAGGAC 
TTAATCAGAA AAGAGGAAGA AAGGAAAAAG ATGGAGAAGC TGCTGGCTGG AGAGGATGGG 
ACAAGGGAAC GAAGGAAAAG CATAAAAACC TACAGAGAAA TCGTCCAAGA AAAAGAGCGG 
AGAGAAAGGG AGTTGCATGA AGCATACAAG AATGCACGGA CCCAGGAGGA GGCAGAGGGT 
ATCCTTCAGC AGTACATCGA GAGATTCACC ATCAGTGAAG CTGTTCTTGA ACGTTTGGAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA ACAGAGCCAA ACTTATCCCC CTTCCTCAGC 
GACCCCAACC CTATGAAATA TCTGCGGCAA CAGTCACTGC CTCCACCCAA GTTCACTGCC 
ACTGTGGAAA CCACCATCGC TCGTCCCAGC GTTCTGGATG CCAGCATGTC AGCAGGCAGT 
GGGTCTCCGA GCAAAACTGT CTCTCCCAAA GCAGTGCCTA TGCTGACACC CAAGCCTTAC 
TCCCAGCCCA AAAACTCTCA AGAGGTCCTG AAGACCTTCA AGGTCGATGG GAAAGTCACC 
ATGAATGGAG AGACGACTCA TGGTGATGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACTTGCCC CCTCGTTGAC CAAATCCCAG ATGTTTGAAG GTGAGGCCAG AGTGCATGGG 
TCCCCCGTGG AGCTGAAACA AGACAGCAAC AGCATTGAAA TCAACATAAA GAAGCCAAGT 
TCTCTTCCCC AAGAGCTGAC AGCCACCACT GAAGAAACTG AAGCGAACAG TCGAGAAGAT 
GAAAATGATG GTGAAGAAGC AGAAAAAGGG AATACAGAAG TTGCCCCATC AGAGCCACAG 
CTTTTTACAA CAACTGTGAC CAGATCCAGC CCAACCGTGG CCTTTGTGGA GCTTTCACCC 
AGCCCTCAGC TGAAGAATGA GGTGCCAGAA GAGAAAGAGC AGAAGAAGCC AGAAAATGAA 
ATGAGTGGGA AGGTGGAGCT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCCGAG 
CCAGAGGCAA CCCTGACATT TCCATTTCTA GACAAAATGC CTGAAACCAA CCAGTCACCT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GCATTCCGCT GAGAGTTCAG 
AACTCTTGGC GACGTTCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG TAGTGAGAAG 
TCACCTGTAA CTACACCTTT TAAGTTCTGG GCCTGGGACC CAGAAGAGGA GCGCAGACGA 
CAGGAAAAAT GGCAACAGGA GCAGGAGCGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAAGA AGAACGCAGA 
TACTATGAGG AGGAGCGGAA AATAATTGAG GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGCTCTGCAG ACCAGCTCTC TACATCTTCC TCTGTGACAG AAGGCAGTGG GACGGTGAAT 
AAGATGGACT TGGAAAACTG TTGTGAAGAA GAACAAGAGA CAAGACAGAG GAGACCACTC 
CAGGAGGATA ACAGTGACTC ACTGCTGAAG ACTAGGGAAG GTGATCCACT GGAGGAGAAG 
GGCAGCAGCC TAACTCAAGA GGTCTTGACT CATTCTGAGA ACCCGGCATC AGAAGGAATC 
TACCACCAGC TGGATACAGA AGCTGGGGCC CCACAGTGTG GGGTAAACCC ACAGCTAGCT 
CAGGATCCAT CCCAGAATCA GCAGGTATCA AACCCACCAA TGCACATTTT AGAAGATGTG 
AAGCCAAAAA CCCTTCCATT GGACAAAAGC ATTAACCATC AGATTGAGTC TCCGAGTGAA 
AGGCGGAAGT CTATAAGTGG GAAGAAGCTG TGCTCTTCGT GTGGGCTTCC ACTGGGTAAA 
GGTGCTGCGA TGATCATTGA GACCCTCAAT CTCTATTTTC ACATCCAGTG CTTTAAGTGT 
GGGATTTGTA AAGGTCAGCT TGGAGACGCC GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTTCTAA ACTGTAATGA CTGCTACATG CGATCCAGAA GTGCTGGCCA GCCCACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

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03-6811-6572

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オンライン注文

LIMCH1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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