limch1b cDNA ORF clone, Danio rerio(zebrafish)

The following limch1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the limch1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148803 XM_021481132.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148804 XM_021481133.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148805 XM_021481134.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148806 XM_021481135.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148807 XM_021481136.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa148808 XM_021481137.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa148809 XM_021481138.1
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa42538 XM_009291003.3
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa42539 XM_009291004.2
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa42540 XM_009291005.2
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42541 XM_009291006.2
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42542 XM_009291007.3
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42543 XM_009291008.3
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42544 XM_009291009.2
Latest version!
Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa42538 XM_009291003.2 Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa42542 XM_009291007.2 Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42543 XM_009291008.2 Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148803
    Clone ID Related Accession (Same CDS sequence) XM_021481132.1
    Accession Version XM_021481132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGTCCCTC CCTCAGTGCT GAATCTGGTG AATCGGGGTG AACACTTTTC TTGGGACCCT
    GAGGAAGAGC GCAATCGTCA GGAGAGGTGG CAGAAAGAGC AGGAACGCAT GCTTCAGGAA
    AAGTATCGAC GTGAGCAGGA GAAGCTAAAG CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG
    GAGGAGGAGG AGAGACGCTA CCATGAGGAG GAGCAGAAGA TTCTGGAAGA GACTGTGACC
    CCTTTGACCC CACACTCACC AATTGCACCG CCAGTCCTTC CCAAAACTCA AAGCACTTTA
    CACTCCCAGC CCGATCGGGA ATATAAGCAG GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG
    AACTGCAGAC AAAGGGAAAA CGGCATCAGT GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA
    CAGATGACAG TAAGTTCTCC CCTCCAAAGC TGTGGGTCAG TTGGTCAGAG AGGACAAGAA
    GACCAGAGCC AAGATAAGCA GCCAACACTA CAGTCTAGTC AGGATATGCC ACTGGGCAAT
    AGACAGATAC CCCAACAGAA TGAGACATGG AAGAAGAATA CAACACCACC AAAACCTGGT
    GGACGCAAGA GGTCCGACTC TATAGAGAAC ATTGGGCCAA ATCCATCAAC ATCACCTTCA
    TTCACAGACA CACAAGCACC TCCATCACCA AACAGGTCTG TCAGTGGGAA GAAACTGTGC
    TCAAGTTGTG GTCACCCACT TGGCAAAGGT GCGGCCATGA TTATAGAGAC TCTCAGCCTT
    TACTTTCACA TCCAGTGCTT TAAGTGTGGA ATCTGTAAGG GTCAGTTGGG TGACACCAGC
    ACAGGAACAG ATGTGAGGAT CAGAAATGGT CTGCTCAACT GCCATCAGTG CTACATCCGC
    TCCCGCTCTG CTGGTCAACC CACTACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336807.1
    CDS164..3796
    Translation

    Target ORF information:

    RefSeq Version XM_021481132.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGTCCCTC CCTCAGTGCT GAATCTGGTG AATCGGGGTG AACACTTTTC TTGGGACCCT
    GAGGAAGAGC GCAATCGTCA GGAGAGGTGG CAGAAAGAGC AGGAACGCAT GCTTCAGGAA
    AAGTATCGAC GTGAGCAGGA GAAGCTAAAG CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG
    GAGGAGGAGG AGAGACGCTA CCATGAGGAG GAGCAGAAGA TTCTGGAAGA GACTGTGACC
    CCTTTGACCC CACACTCACC AATTGCACCG CCAGTCCTTC CCAAAACTCA AAGCACTTTA
    CACTCCCAGC CCGATCGGGA ATATAAGCAG GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG
    AACTGCAGAC AAAGGGAAAA CGGCATCAGT GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA
    CAGATGACAG TAAGTTCTCC CCTCCAAAGC TGTGGGTCAG TTGGTCAGAG AGGACAAGAA
    GACCAGAGCC AAGATAAGCA GCCAACACTA CAGTCTAGTC AGGATATGCC ACTGGGCAAT
    AGACAGATAC CCCAACAGAA TGAGACATGG AAGAAGAATA CAACACCACC AAAACCTGGT
    GGACGCAAGA GGTCCGACTC TATAGAGAAC ATTGGGCCAA ATCCATCAAC ATCACCTTCA
    TTCACAGACA CACAAGCACC TCCATCACCA AACAGGTCTG TCAGTGGGAA GAAACTGTGC
    TCAAGTTGTG GTCACCCACT TGGCAAAGGT GCGGCCATGA TTATAGAGAC TCTCAGCCTT
    TACTTTCACA TCCAGTGCTT TAAGTGTGGA ATCTGTAAGG GTCAGTTGGG TGACACCAGC
    ACAGGAACAG ATGTGAGGAT CAGAAATGGT CTGCTCAACT GCCATCAGTG CTACATCCGC
    TCCCGCTCTG CTGGTCAACC CACTACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148804
    Clone ID Related Accession (Same CDS sequence) XM_021481133.1
    Accession Version XM_021481133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGATATGGCT GAGAAGGCTC ATGAAAATGT CCCTCCCTCA
    GTGCTGAATC TGGTGAATCG GGGTGAACAC TTTTCTTGGG ACCCTGAGGA AGAGCGCAAT
    CGTCAGGAGA GGTGGCAGAA AGAGCAGGAA CGCATGCTTC AGGAAAAGTA TCGACGTGAG
    CAGGAGAAGC TAAAGCAGGA GTGGGAGCAG GCGCAGAAGG AGGTGGAGGA GGAGGAGAGA
    CGCTACCATG AGGAGGAGCA GAAGATTCTG GAAGAGACTG TGACCCCTTT GACCCCACAC
    TCACCAATTG CACCGCCAGT CCTTCCCAAA ACTCAAAGCA CTTTACACTC CCAGCCCGAT
    CGGGAATATA AGCAGGGGCC AGTGGAGAAG CAGCCGAGTT CTGAGAACTG CAGACAAAGG
    GAAAACGGCA TCAGTGAAGC AGCACAAAGA AAGACGGAGA GTTTACAGAT GACAGTAAGT
    TCTCCCCTCC AAAGCTGTGG GTCAGTTGGT CAGAGAGGAC AAGAAGACCA GAGCCAAGAT
    AAGCAGCCAA CACTACAGTC TAGTCAGGAT ATGCCACTGG GCAATAGACA GATACCCCAA
    CAGAATGAGA CATGGAAGAA GAATACAACA CCACCAAAAC CTGGTGGACG CAAGAGGTCC
    GACTCTATAG AGAACATTGG GCCAAATCCA TCAACATCAC CTTCATTCAC AGACACACAA
    GCACCTCCAT CACCAAACAG GTCTGTCAGT GGGAAGAAAC TGTGCTCAAG TTGTGGTCAC
    CCACTTGGCA AAGGTGCGGC CATGATTATA GAGACTCTCA GCCTTTACTT TCACATCCAG
    TGCTTTAAGT GTGGAATCTG TAAGGGTCAG TTGGGTGACA CCAGCACAGG AACAGATGTG
    AGGATCAGAA ATGGTCTGCT CAACTGCCAT CAGTGCTACA TCCGCTCCCG CTCTGCTGGT
    CAACCCACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336808.1
    CDS163..3720
    Translation

    Target ORF information:

    RefSeq Version XM_021481133.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGATATGGCT GAGAAGGCTC ATGAAAATGT CCCTCCCTCA
    GTGCTGAATC TGGTGAATCG GGGTGAACAC TTTTCTTGGG ACCCTGAGGA AGAGCGCAAT
    CGTCAGGAGA GGTGGCAGAA AGAGCAGGAA CGCATGCTTC AGGAAAAGTA TCGACGTGAG
    CAGGAGAAGC TAAAGCAGGA GTGGGAGCAG GCGCAGAAGG AGGTGGAGGA GGAGGAGAGA
    CGCTACCATG AGGAGGAGCA GAAGATTCTG GAAGAGACTG TGACCCCTTT GACCCCACAC
    TCACCAATTG CACCGCCAGT CCTTCCCAAA ACTCAAAGCA CTTTACACTC CCAGCCCGAT
    CGGGAATATA AGCAGGGGCC AGTGGAGAAG CAGCCGAGTT CTGAGAACTG CAGACAAAGG
    GAAAACGGCA TCAGTGAAGC AGCACAAAGA AAGACGGAGA GTTTACAGAT GACAGTAAGT
    TCTCCCCTCC AAAGCTGTGG GTCAGTTGGT CAGAGAGGAC AAGAAGACCA GAGCCAAGAT
    AAGCAGCCAA CACTACAGTC TAGTCAGGAT ATGCCACTGG GCAATAGACA GATACCCCAA
    CAGAATGAGA CATGGAAGAA GAATACAACA CCACCAAAAC CTGGTGGACG CAAGAGGTCC
    GACTCTATAG AGAACATTGG GCCAAATCCA TCAACATCAC CTTCATTCAC AGACACACAA
    GCACCTCCAT CACCAAACAG GTCTGTCAGT GGGAAGAAAC TGTGCTCAAG TTGTGGTCAC
    CCACTTGGCA AAGGTGCGGC CATGATTATA GAGACTCTCA GCCTTTACTT TCACATCCAG
    TGCTTTAAGT GTGGAATCTG TAAGGGTCAG TTGGGTGACA CCAGCACAGG AACAGATGTG
    AGGATCAGAA ATGGTCTGCT CAACTGCCAT CAGTGCTACA TCCGCTCCCG CTCTGCTGGT
    CAACCCACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148805
    Clone ID Related Accession (Same CDS sequence) XM_021481134.1
    Accession Version XM_021481134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGTCCCTC CCTCAGTGCT GAATCTGGTG AATCGGGGTG AACACTTTTC TTGGGACCCT
    GAGGAAGAGC GCAATCGTCA GGAGAGGTGG CAGAAAGAGC AGGAACGCAT GCTTCAGGAA
    AAGTATCGAC GTGAGCAGGA GAAGCTAAAG CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG
    GAGGAGGAGG AGAGACGCTA CCATGAGGAG GAGCAGAAGA TTCTGGAAGA GACTGTGACC
    CCTTTGACCC CACACTCACC AATTGCACCG CCAGTCCTTC CCAAAACTCA AAGCACTTTA
    CACTCCCAGC CCGATCGGGA ATATAAGCAG GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG
    AACTGCAGAC AAAGGGAAAA CGGCATCAGT GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA
    CAGATGACAG TAAGTTCTCC CCTCCAAAGC TGTGGGTCAG TTGGTCAGAG AGGACAAGAA
    GACCAGAGCC AAGATAAGCA GCCAACACTA CAGTCTAGTC AGGATATGCC ACTGGGCAAT
    AGACAGATAC CCCAACAGAA TGAGACATGG AAGAAGAATA CAACACCACC AAAACCTGGT
    GGACGCAAGA GGTCTGTCAG TGGGAAGAAA CTGTGCTCAA GTTGTGGTCA CCCACTTGGC
    AAAGGTGCGG CCATGATTAT AGAGACTCTC AGCCTTTACT TTCACATCCA GTGCTTTAAG
    TGTGGAATCT GTAAGGGTCA GTTGGGTGAC ACCAGCACAG GAACAGATGT GAGGATCAGA
    AATGGTCTGC TCAACTGCCA TCAGTGCTAC ATCCGCTCCC GCTCTGCTGG TCAACCCACT
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336809.1
    CDS165..3713
    Translation

    Target ORF information:

    RefSeq Version XM_021481134.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGTCCCTC CCTCAGTGCT GAATCTGGTG AATCGGGGTG AACACTTTTC TTGGGACCCT
    GAGGAAGAGC GCAATCGTCA GGAGAGGTGG CAGAAAGAGC AGGAACGCAT GCTTCAGGAA
    AAGTATCGAC GTGAGCAGGA GAAGCTAAAG CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG
    GAGGAGGAGG AGAGACGCTA CCATGAGGAG GAGCAGAAGA TTCTGGAAGA GACTGTGACC
    CCTTTGACCC CACACTCACC AATTGCACCG CCAGTCCTTC CCAAAACTCA AAGCACTTTA
    CACTCCCAGC CCGATCGGGA ATATAAGCAG GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG
    AACTGCAGAC AAAGGGAAAA CGGCATCAGT GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA
    CAGATGACAG TAAGTTCTCC CCTCCAAAGC TGTGGGTCAG TTGGTCAGAG AGGACAAGAA
    GACCAGAGCC AAGATAAGCA GCCAACACTA CAGTCTAGTC AGGATATGCC ACTGGGCAAT
    AGACAGATAC CCCAACAGAA TGAGACATGG AAGAAGAATA CAACACCACC AAAACCTGGT
    GGACGCAAGA GGTCTGTCAG TGGGAAGAAA CTGTGCTCAA GTTGTGGTCA CCCACTTGGC
    AAAGGTGCGG CCATGATTAT AGAGACTCTC AGCCTTTACT TTCACATCCA GTGCTTTAAG
    TGTGGAATCT GTAAGGGTCA GTTGGGTGAC ACCAGCACAG GAACAGATGT GAGGATCAGA
    AATGGTCTGC TCAACTGCCA TCAGTGCTAC ATCCGCTCCC GCTCTGCTGG TCAACCCACT
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148806
    Clone ID Related Accession (Same CDS sequence) XM_021481135.1
    Accession Version XM_021481135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGAAAAGT ATCGACGTGA GCAGGAGAAG CTAAAGCAGG AGTGGGAGCA GGCGCAGAAG
    GAGGTGGAGG AGGAGGAGAG ACGCTACCAT GAGGAGGAGC AGAAGATTCT GGAAGAGACT
    GTGACCCCTT TGACCCCACA CTCACCAATT GCACCGCCAG TCCTTCCCAA AACTCAAAGC
    ACTTTACACT CCCAGCCCGA TCGGGAATAT AAGCAGGGGC CAGTGGAGAA GCAGCCGAGT
    TCTGAGAACT GCAGACAAAG GGAAAACGGC ATCAGTGAAG CAGCACAAAG AAAGACGGAG
    AGTTTACAGA TGACAGTAAG TTCTCCCCTC CAAAGCTGTG GGTCAGTTGG TCAGAGAGGA
    CAAGAAGACC AGAGCCAAGA TAAGCAGCCA ACACTACAGT CTAGTCAGGA TATGCCACTG
    GGCAATAGAC AGATACCCCA ACAGAATGAG ACATGGAAGA AGAATACAAC ACCACCAAAA
    CCTGGTGGAC GCAAGAGGTC CGACTCTATA GAGAACATTG GGCCAAATCC ATCAACATCA
    CCTTCATTCA CAGACACACA AGCACCTCCA TCACCAAACA GGTCTGTCAG TGGGAAGAAA
    CTGTGCTCAA GTTGTGGTCA CCCACTTGGC AAAGGTGCGG CCATGATTAT AGAGACTCTC
    AGCCTTTACT TTCACATCCA GTGCTTTAAG TGTGGAATCT GTAAGGGTCA GTTGGGTGAC
    ACCAGCACAG GAACAGATGT GAGGATCAGA AATGGTCTGC TCAACTGCCA TCAGTGCTAC
    ATCCGCTCCC GCTCTGCTGG TCAACCCACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336810.1
    CDS163..3681
    Translation

    Target ORF information:

    RefSeq Version XM_021481135.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGAGTTGGCT GTCAGTGCTC AGTTAACATC GTTGTCAAGT
    AATCCTAAAT TGCGCAGAGA TTTCTTCGCC CAATCAGATA TGGCTGAGAA GGCTCATGAA
    AATGAAAAGT ATCGACGTGA GCAGGAGAAG CTAAAGCAGG AGTGGGAGCA GGCGCAGAAG
    GAGGTGGAGG AGGAGGAGAG ACGCTACCAT GAGGAGGAGC AGAAGATTCT GGAAGAGACT
    GTGACCCCTT TGACCCCACA CTCACCAATT GCACCGCCAG TCCTTCCCAA AACTCAAAGC
    ACTTTACACT CCCAGCCCGA TCGGGAATAT AAGCAGGGGC CAGTGGAGAA GCAGCCGAGT
    TCTGAGAACT GCAGACAAAG GGAAAACGGC ATCAGTGAAG CAGCACAAAG AAAGACGGAG
    AGTTTACAGA TGACAGTAAG TTCTCCCCTC CAAAGCTGTG GGTCAGTTGG TCAGAGAGGA
    CAAGAAGACC AGAGCCAAGA TAAGCAGCCA ACACTACAGT CTAGTCAGGA TATGCCACTG
    GGCAATAGAC AGATACCCCA ACAGAATGAG ACATGGAAGA AGAATACAAC ACCACCAAAA
    CCTGGTGGAC GCAAGAGGTC CGACTCTATA GAGAACATTG GGCCAAATCC ATCAACATCA
    CCTTCATTCA CAGACACACA AGCACCTCCA TCACCAAACA GGTCTGTCAG TGGGAAGAAA
    CTGTGCTCAA GTTGTGGTCA CCCACTTGGC AAAGGTGCGG CCATGATTAT AGAGACTCTC
    AGCCTTTACT TTCACATCCA GTGCTTTAAG TGTGGAATCT GTAAGGGTCA GTTGGGTGAC
    ACCAGCACAG GAACAGATGT GAGGATCAGA AATGGTCTGC TCAACTGCCA TCAGTGCTAC
    ATCCGCTCCC GCTCTGCTGG TCAACCCACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148807
    Clone ID Related Accession (Same CDS sequence) XM_021481136.1
    Accession Version XM_021481136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGATATGGCT GAGAAGGCTC ATGAAAATGA AAAGTATCGA
    CGTGAGCAGG AGAAGCTAAA GCAGGAGTGG GAGCAGGCGC AGAAGGAGGT GGAGGAGGAG
    GAGAGACGCT ACCATGAGGA GGAGCAGAAG ATTCTGGAAG AGACTGTGAC CCCTTTGACC
    CCACACTCAC CAATTGCACC GCCAGTCCTT CCCAAAACTC AAAGCACTTT ACACTCCCAG
    CCCGATCGGG AATATAAGCA GGGGCCAGTG GAGAAGCAGC CGAGTTCTGA GAACTGCAGA
    CAAAGGGAAA ACGGCATCAG TGAAGCAGCA CAAAGAAAGA CGGAGAGTTT ACAGATGACA
    GTAAGTTCTC CCCTCCAAAG CTGTGGGTCA GTTGGTCAGA GAGGACAAGA AGACCAGAGC
    CAAGATAAGC AGCCAACACT ACAGTCTAGT CAGGATATGC CACTGGGCAA TAGACAGATA
    CCCCAACAGA ATGAGACATG GAAGAAGAAT ACAACACCAC CAAAACCTGG TGGACGCAAG
    AGGTCCGACT CTATAGAGAA CATTGGGCCA AATCCATCAA CATCACCTTC ATTCACAGAC
    ACACAAGCAC CTCCATCACC AAACAGGTCT GTCAGTGGGA AGAAACTGTG CTCAAGTTGT
    GGTCACCCAC TTGGCAAAGG TGCGGCCATG ATTATAGAGA CTCTCAGCCT TTACTTTCAC
    ATCCAGTGCT TTAAGTGTGG AATCTGTAAG GGTCAGTTGG GTGACACCAG CACAGGAACA
    GATGTGAGGA TCAGAAATGG TCTGCTCAAC TGCCATCAGT GCTACATCCG CTCCCGCTCT
    GCTGGTCAAC CCACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336811.1
    CDS162..3605
    Translation

    Target ORF information:

    RefSeq Version XM_021481136.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGATTCAG CCGTTCATCT
    AGTGTGTTTG ATGATCCAGA GAGCGTGAGC ATGATTGACA TGAGAAGCGA GGAAGACTTG
    TCACCACATA GTCAAGTTCG ACACGAGCTC ATGCACAACC AGTACAACAA GCTGAAAGAG
    GAAGAAGACC ACTGGCAGGA TGATTTGGCC AAATGGAAGA GTCGCAGGAG GAGCGTGTCA
    CAGGACCTGA TCAAAAAAGA AGAGGAGAGG AAGATGATGG AGAGACTGCT AAGTGGAGAT
    GGAGGATCAC AGAGAAGGAA AAGCATTAAG ACGTACAGAG AAATAGTAGA GGAGAAGGAG
    CGTCGTGAAC AGGAGCTGCA TGACGCTTAT CGAAAGGCTC GCACACCAGA GGAGGCAGCA
    GCTGTGCTGC ATCGCTACGC CCAGCGATTC ACCATCAGCG AGGCTGTTCT GGAGCGCCTC
    AAACTGCCAA AGCTGCTGGA GAGGAGTGTG TCGGCTGATC CCACTCGTCA CTCCTCTCTC
    CCCATCTCCC AGGACATCTC GTCCTCTTTC AGCGCTTCCC CTAATGTGCT GCAGTACCTA
    AGGCAGCAGT CTCTTCCCGC AGCTAAGTTC ACATCCATGG TCGAGGCAAG GGTGACGGGA
    TACAGCCCAG AAAAGGGAAT TGTGGGAGAT GCAGAGTGTG TCTCAGATCC ATCTCGCTCA
    CATGGGCCAT CACTGGGTCC GAAACCTTAC AGACAGAGCA GAGCTGCCAC TGCTGGGATG
    AGAACAGTAA AGTTGGATGA AAGAGTGCGA GTGAATGGGG AAAGGGAAGA TGACGGCCTC
    GGCATTGAGA AGACAGGACC ACAGAGAAAC AAAGCAGAGG AAGAAGATTC TTCTCATCTA
    CAGTTCTTCT CATCTTCCTG CCAGCACACA GACTCAGACA TGACAGATAG TGAAGTCTCT
    GTAGTTCAGC CCACGGTCCA CATGCTCTCA TCCACACGCA CAGCAGAGAG CCCAGAGAAA
    CACAGAGATA CTACAGAGAT TGAAGGACGG ACGGTCCCGC AGGTCGGAGA TGTCCCTGAA
    TTCCCAAAAG TGTTGGAGAT AAATCCAGAT GAGACTGTTA CAGATCAAGA GAAGACAGAT
    GAGAGGGGTA TGAGTGACTC TCTACACGAC GTGATTCAAA TGGAGATTTC TGCACAGGTC
    AGTGAACTAA CAGCTGAGAT CCCACAGAAA TCAACAAATG AATCTGAGTC ATCCAGACCC
    GAATCATACA GAACCAAAGC AGATATGGCT GAGAAGGCTC ATGAAAATGA AAAGTATCGA
    CGTGAGCAGG AGAAGCTAAA GCAGGAGTGG GAGCAGGCGC AGAAGGAGGT GGAGGAGGAG
    GAGAGACGCT ACCATGAGGA GGAGCAGAAG ATTCTGGAAG AGACTGTGAC CCCTTTGACC
    CCACACTCAC CAATTGCACC GCCAGTCCTT CCCAAAACTC AAAGCACTTT ACACTCCCAG
    CCCGATCGGG AATATAAGCA GGGGCCAGTG GAGAAGCAGC CGAGTTCTGA GAACTGCAGA
    CAAAGGGAAA ACGGCATCAG TGAAGCAGCA CAAAGAAAGA CGGAGAGTTT ACAGATGACA
    GTAAGTTCTC CCCTCCAAAG CTGTGGGTCA GTTGGTCAGA GAGGACAAGA AGACCAGAGC
    CAAGATAAGC AGCCAACACT ACAGTCTAGT CAGGATATGC CACTGGGCAA TAGACAGATA
    CCCCAACAGA ATGAGACATG GAAGAAGAAT ACAACACCAC CAAAACCTGG TGGACGCAAG
    AGGTCCGACT CTATAGAGAA CATTGGGCCA AATCCATCAA CATCACCTTC ATTCACAGAC
    ACACAAGCAC CTCCATCACC AAACAGGTCT GTCAGTGGGA AGAAACTGTG CTCAAGTTGT
    GGTCACCCAC TTGGCAAAGG TGCGGCCATG ATTATAGAGA CTCTCAGCCT TTACTTTCAC
    ATCCAGTGCT TTAAGTGTGG AATCTGTAAG GGTCAGTTGG GTGACACCAG CACAGGAACA
    GATGTGAGGA TCAGAAATGG TCTGCTCAAC TGCCATCAGT GCTACATCCG CTCCCGCTCT
    GCTGGTCAAC CCACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148808
    Clone ID Related Accession (Same CDS sequence) XM_021481137.1
    Accession Version XM_021481137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAAAGG AAGCAGAAGA GTGGGACAGC CCGAAGCGTA GCATCAGGGA CAGTGGTTAT 
    ATAGACTGCT GGGAGTCTGA GCGCAGCGAT TCTCTCTCTC CGCCGAGGCA CGCGCGAGAC
    GACTCCTTCG ACAGCCTGGA CTCCATCGGC TCACGCTCCC ATCAAACCCC CTCGCCCGAC
    GGCCTGCTCG CCCGCGGCTA CAGCGATGGC CGCGGCAGCG ACTCGGAGTG CGACACCCCC
    CAGAGGAAGA TACCGGATGT ACGCAAGGAT GACATGTTGG TGCGGCGAAC ATCCTGCAGT
    GAGCCACGGA CCGCTGTTCC TTTCAATCAG TACCTGCCCA ACAAAGCCAA CCAGGGTTCC
    TATGTGCCCC CACCAGTGCG ACGCCCGCGT GCCGACAGAG ACGAAAGCCG CAAAAGCTGG
    AGTAACGCAA CCTCACCTGT TGGGGGAGAG AGACCTTTCA GTGAGAGTGA AACCCTTTCG
    TTAGAGTTGT CCGCTGTGGT AACAGGACGT GATGTTCACT GTGTAGATTC ACAGCAAATA
    GATCCTGACT CTAAATACTC CAGAGCGTCT CCTCAAAACG AGTCTCCCCC CAAAGACCCA
    ATTTTTCTTA ATCCACAACT CAGTGACCCT CAGATTAGAT CCCCCCTTCC CAAAGACACC
    CTCCATCCTT CTGTCCAACC CCAACCTCAA GAGCAGAGCC ACTCAGACCA GGAGAAGATG
    GAGGAGGAGG AAAAGGACAG AGATGGATGG GCCACTTTCT GCCAAAGTCC TCCAAACCCC
    TTGGATACCC TTCAGAGCGA CTTACAGATG TCCCATAATC CGACTGCAGG AAATGATACC
    AGATTCAGCC GTTCATCTAG TGTGTTTGAT GATCCAGAGA GCGTGAGCAT GATTGACATG
    AGAAGCGAGG AAGACTTGTC ACCACATAGT CAAGTTCGAC ACGAGCTCAT GCACAACCAG
    TACAACAAGC TGAAAGAGGA AGAAGACCAC TGGCAGGATG ATTTGGCCAA ATGGAAGAGT
    CGCAGGAGGA GCGTGTCACA GGACCTGATC AAAAAAGAAG AGGAGAGGAA GATGATGGAG
    AGACTGCTAA GTGGAGATGG AGGATCACAG AGAAGGAAAA GCATTAAGAC GTACAGAGAA
    ATAGTAGAGG AGAAGGAGCG TCGTGAACAG GAGCTGCATG ACGCTTATCG AAAGGCTCGC
    ACACCAGAGG AGGCAGCAGC TGTGCTGCAT CGCTACGCCC AGCGATTCAC CATCAGCGAG
    GCTGTTCTGG AGCGCCTCAA ACTGCCAAAG CTGCTGGAGA GGAGTGTGTC GGCTGATCCC
    ACTCGTCACT CCTCTCTCCC CATCTCCCAG GACATCTCGT CCTCTTTCAG CGCTTCCCCT
    AATGTGCTGC AGTACCTAAG GCAGCAGTCT CTTCCCGCAG CTAAGTTCAC ATCCATGGTC
    GAGGCAAGGG TGACGGGATA CAGCCCAGAA AAGGGAATTG TGGGAGATGC AGAGTGTGTC
    TCAGATCCAT CTCGCTCACA TGGGCCATCA CTGGGTCCGA AACCTTACAG ACAGAGCAGA
    GCTGCCACTG CTGGGATGAG AACAGTAAAG TTGGATGAAA GAGTGCGAGT GAATGGGGAA
    AGGGAAGATG ACGGCCTCGG CATTGAGAAG ACAGGACCAC AGAGAAACAA AGCAGAGGAA
    GAAGATTCTT CTCATCTACA GTTCTTCTCA TCTTCCTGCC AGCACACAGA CTCAGACATG
    ACAGATAGTG AAGTCTCTGT AGTTCAGCCC ACGGTCCACA TGCTCTCATC CACACGCACA
    GCAGAGAGCC CAGAGAAACA CAGAGATACT ACAGAGATTG AAGGACGGAC GGTCCCGCAG
    GTCGGAGATG TCCCTGAATT CCCAAAAGTG TTGGAGATAA ATCCAGATGA GACTGTTACA
    GATCAAGAGA AGACAGATGA GAGGGGTATG AGTGACTCTC TACACGACGT GATTCAAATG
    GAGATTTCTG CACAGGTCAG TGAACTAACA GCTGAGATCC CACAGAAATC AACAAATGAA
    TCTGAGTCAT CCAGACCCGA ATCATACAGA ACCAAAGCAG AGTTGGCTGT CAGTGCTCAG
    TTAACATCGT TGTCAAGTAA TCCTAAATTG CGCAGAGATT TCTTCGCCCA ATCAGATATG
    GCTGAGAAGG CTCATGAAAA TGTCCCTCCC TCAGTGCTGA ATCTGGTGAA TCGGGGTGAA
    CACTTTTCTT GGGACCCTGA GGAAGAGCGC AATCGTCAGG AGAGGTGGCA GAAAGAGCAG
    GAACGCATGC TTCAGGAAAA GTATCGACGT GAGCAGGAGA AGCTAAAGCA GGAGTGGGAG
    CAGGCGCAGA AGGAGGTGGA GGAGGAGGAG AGACGCTACC ATGAGGAGGA GCAGAAGATT
    CTGGAAGAGA CTGTGACCCC TTTGACCCCA CACTCACCAA TTGCACCGCC AGTCCTTCCC
    AAAACTCAAA GCACTTTACA CTCCCAGCCC GATCGGGAAT ATAAGCAGGG GCCAGTGGAG
    AAGCAGCCGA GTTCTGAGAA CTGCAGACAA AGGGAAAACG GCATCAGTGA AGCAGCACAA
    AGAAAGACGG AGAGTTTACA GATGACAGTA AGTTCTCCCC TCCAAAGCTG TGGGTCAGTT
    GGTCAGAGAG GACAAGAAGA CCAGAGCCAA GATAAGCAGC CAACACTACA GTCTAGTCAG
    GATATGCCAC TGGGCAATAG ACAGATACCC CAACAGAATG AGACATGGAA GAAGAATACA
    ACACCACCAA AACCTGGTGG ACGCAAGAGG TCCGACTCTA TAGAGAACAT TGGGCCAAAT
    CCATCAACAT CACCTTCATT CACAGACACA CAAGCACCTC CATCACCAAA CAGGTCTGTC
    AGTGGGAAGA AACTGTGCTC AAGTTGTGGT CACCCACTTG GCAAAGGTGC GGCCATGATT
    ATAGAGACTC TCAGCCTTTA CTTTCACATC CAGTGCTTTA AGTGTGGAAT CTGTAAGGGT
    CAGTTGGGTG ACACCAGCAC AGGAACAGAT GTGAGGATCA GAAATGGTCT GCTCAACTGC
    CATCAGTGCT ACATCCGCTC CCGCTCTGCT GGTCAACCCA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336812.1
    CDS369..3479
    Translation

    Target ORF information:

    RefSeq Version XM_021481137.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAAAGG AAGCAGAAGA GTGGGACAGC CCGAAGCGTA GCATCAGGGA CAGTGGTTAT 
    ATAGACTGCT GGGAGTCTGA GCGCAGCGAT TCTCTCTCTC CGCCGAGGCA CGCGCGAGAC
    GACTCCTTCG ACAGCCTGGA CTCCATCGGC TCACGCTCCC ATCAAACCCC CTCGCCCGAC
    GGCCTGCTCG CCCGCGGCTA CAGCGATGGC CGCGGCAGCG ACTCGGAGTG CGACACCCCC
    CAGAGGAAGA TACCGGATGT ACGCAAGGAT GACATGTTGG TGCGGCGAAC ATCCTGCAGT
    GAGCCACGGA CCGCTGTTCC TTTCAATCAG TACCTGCCCA ACAAAGCCAA CCAGGGTTCC
    TATGTGCCCC CACCAGTGCG ACGCCCGCGT GCCGACAGAG ACGAAAGCCG CAAAAGCTGG
    AGTAACGCAA CCTCACCTGT TGGGGGAGAG AGACCTTTCA GTGAGAGTGA AACCCTTTCG
    TTAGAGTTGT CCGCTGTGGT AACAGGACGT GATGTTCACT GTGTAGATTC ACAGCAAATA
    GATCCTGACT CTAAATACTC CAGAGCGTCT CCTCAAAACG AGTCTCCCCC CAAAGACCCA
    ATTTTTCTTA ATCCACAACT CAGTGACCCT CAGATTAGAT CCCCCCTTCC CAAAGACACC
    CTCCATCCTT CTGTCCAACC CCAACCTCAA GAGCAGAGCC ACTCAGACCA GGAGAAGATG
    GAGGAGGAGG AAAAGGACAG AGATGGATGG GCCACTTTCT GCCAAAGTCC TCCAAACCCC
    TTGGATACCC TTCAGAGCGA CTTACAGATG TCCCATAATC CGACTGCAGG AAATGATACC
    AGATTCAGCC GTTCATCTAG TGTGTTTGAT GATCCAGAGA GCGTGAGCAT GATTGACATG
    AGAAGCGAGG AAGACTTGTC ACCACATAGT CAAGTTCGAC ACGAGCTCAT GCACAACCAG
    TACAACAAGC TGAAAGAGGA AGAAGACCAC TGGCAGGATG ATTTGGCCAA ATGGAAGAGT
    CGCAGGAGGA GCGTGTCACA GGACCTGATC AAAAAAGAAG AGGAGAGGAA GATGATGGAG
    AGACTGCTAA GTGGAGATGG AGGATCACAG AGAAGGAAAA GCATTAAGAC GTACAGAGAA
    ATAGTAGAGG AGAAGGAGCG TCGTGAACAG GAGCTGCATG ACGCTTATCG AAAGGCTCGC
    ACACCAGAGG AGGCAGCAGC TGTGCTGCAT CGCTACGCCC AGCGATTCAC CATCAGCGAG
    GCTGTTCTGG AGCGCCTCAA ACTGCCAAAG CTGCTGGAGA GGAGTGTGTC GGCTGATCCC
    ACTCGTCACT CCTCTCTCCC CATCTCCCAG GACATCTCGT CCTCTTTCAG CGCTTCCCCT
    AATGTGCTGC AGTACCTAAG GCAGCAGTCT CTTCCCGCAG CTAAGTTCAC ATCCATGGTC
    GAGGCAAGGG TGACGGGATA CAGCCCAGAA AAGGGAATTG TGGGAGATGC AGAGTGTGTC
    TCAGATCCAT CTCGCTCACA TGGGCCATCA CTGGGTCCGA AACCTTACAG ACAGAGCAGA
    GCTGCCACTG CTGGGATGAG AACAGTAAAG TTGGATGAAA GAGTGCGAGT GAATGGGGAA
    AGGGAAGATG ACGGCCTCGG CATTGAGAAG ACAGGACCAC AGAGAAACAA AGCAGAGGAA
    GAAGATTCTT CTCATCTACA GTTCTTCTCA TCTTCCTGCC AGCACACAGA CTCAGACATG
    ACAGATAGTG AAGTCTCTGT AGTTCAGCCC ACGGTCCACA TGCTCTCATC CACACGCACA
    GCAGAGAGCC CAGAGAAACA CAGAGATACT ACAGAGATTG AAGGACGGAC GGTCCCGCAG
    GTCGGAGATG TCCCTGAATT CCCAAAAGTG TTGGAGATAA ATCCAGATGA GACTGTTACA
    GATCAAGAGA AGACAGATGA GAGGGGTATG AGTGACTCTC TACACGACGT GATTCAAATG
    GAGATTTCTG CACAGGTCAG TGAACTAACA GCTGAGATCC CACAGAAATC AACAAATGAA
    TCTGAGTCAT CCAGACCCGA ATCATACAGA ACCAAAGCAG AGTTGGCTGT CAGTGCTCAG
    TTAACATCGT TGTCAAGTAA TCCTAAATTG CGCAGAGATT TCTTCGCCCA ATCAGATATG
    GCTGAGAAGG CTCATGAAAA TGTCCCTCCC TCAGTGCTGA ATCTGGTGAA TCGGGGTGAA
    CACTTTTCTT GGGACCCTGA GGAAGAGCGC AATCGTCAGG AGAGGTGGCA GAAAGAGCAG
    GAACGCATGC TTCAGGAAAA GTATCGACGT GAGCAGGAGA AGCTAAAGCA GGAGTGGGAG
    CAGGCGCAGA AGGAGGTGGA GGAGGAGGAG AGACGCTACC ATGAGGAGGA GCAGAAGATT
    CTGGAAGAGA CTGTGACCCC TTTGACCCCA CACTCACCAA TTGCACCGCC AGTCCTTCCC
    AAAACTCAAA GCACTTTACA CTCCCAGCCC GATCGGGAAT ATAAGCAGGG GCCAGTGGAG
    AAGCAGCCGA GTTCTGAGAA CTGCAGACAA AGGGAAAACG GCATCAGTGA AGCAGCACAA
    AGAAAGACGG AGAGTTTACA GATGACAGTA AGTTCTCCCC TCCAAAGCTG TGGGTCAGTT
    GGTCAGAGAG GACAAGAAGA CCAGAGCCAA GATAAGCAGC CAACACTACA GTCTAGTCAG
    GATATGCCAC TGGGCAATAG ACAGATACCC CAACAGAATG AGACATGGAA GAAGAATACA
    ACACCACCAA AACCTGGTGG ACGCAAGAGG TCCGACTCTA TAGAGAACAT TGGGCCAAAT
    CCATCAACAT CACCTTCATT CACAGACACA CAAGCACCTC CATCACCAAA CAGGTCTGTC
    AGTGGGAAGA AACTGTGCTC AAGTTGTGGT CACCCACTTG GCAAAGGTGC GGCCATGATT
    ATAGAGACTC TCAGCCTTTA CTTTCACATC CAGTGCTTTA AGTGTGGAAT CTGTAAGGGT
    CAGTTGGGTG ACACCAGCAC AGGAACAGAT GTGAGGATCA GAAATGGTCT GCTCAACTGC
    CATCAGTGCT ACATCCGCTC CCGCTCTGCT GGTCAACCCA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148809
    Clone ID Related Accession (Same CDS sequence) XM_021481138.1
    Accession Version XM_021481138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GATATGGCTG AGAAGGCTCA TGAAAATGAA AAGTATCGAC GTGAGCAGGA GAAGCTAAAG
    CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG GAGGAGGAGG AGAGACGCTA CCATGAGGAG
    GAGCAGAAGA TTCTGGAAGA GACTGTGACC CCTTTGACCC CACACTCACC AATTGCACCG
    CCAGTCCTTC CCAAAACTCA AAGCACTTTA CACTCCCAGC CCGATCGGGA ATATAAGCAG
    GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG AACTGCAGAC AAAGGGAAAA CGGCATCAGT
    GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA CAGATGACAG TAAGTTCTCC CCTCCAAAGC
    TGTGGGTCAG TTGGTCAGAG AGGACAAGAA GACCAGAGCC AAGATAAGCA GCCAACACTA
    CAGTCTAGTC AGGATATGCC ACTGGGCAAT AGACAGATAC CCCAACAGAA TGAGACATGG
    AAGAAGAATA CAACACCACC AAAACCTGGT GGACGCAAGA GGTCCGACTC TATAGAGAAC
    ATTGGGCCAA ATCCATCAAC ATCACCTTCA TTCACAGACA CACAAGCACC TCCATCACCA
    AACAGGTCTG TCAGTGGGAA GAAACTGTGC TCAAGTTGTG GTCACCCACT TGGCAAAGGT
    GCGGCCATGA TTATAGAGAC TCTCAGCCTT TACTTTCACA TCCAGTGCTT TAAGTGTGGA
    ATCTGTAAGG GTCAGTTGGG TGACACCAGC ACAGGAACAG ATGTGAGGAT CAGAAATGGT
    CTGCTCAACT GCCATCAGTG CTACATCCGC TCCCGCTCTG CTGGTCAACC CACTACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336813.1
    CDS115..3177
    Translation

    Target ORF information:

    RefSeq Version XM_021481138.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GATATGGCTG AGAAGGCTCA TGAAAATGAA AAGTATCGAC GTGAGCAGGA GAAGCTAAAG
    CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG GAGGAGGAGG AGAGACGCTA CCATGAGGAG
    GAGCAGAAGA TTCTGGAAGA GACTGTGACC CCTTTGACCC CACACTCACC AATTGCACCG
    CCAGTCCTTC CCAAAACTCA AAGCACTTTA CACTCCCAGC CCGATCGGGA ATATAAGCAG
    GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG AACTGCAGAC AAAGGGAAAA CGGCATCAGT
    GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA CAGATGACAG TAAGTTCTCC CCTCCAAAGC
    TGTGGGTCAG TTGGTCAGAG AGGACAAGAA GACCAGAGCC AAGATAAGCA GCCAACACTA
    CAGTCTAGTC AGGATATGCC ACTGGGCAAT AGACAGATAC CCCAACAGAA TGAGACATGG
    AAGAAGAATA CAACACCACC AAAACCTGGT GGACGCAAGA GGTCCGACTC TATAGAGAAC
    ATTGGGCCAA ATCCATCAAC ATCACCTTCA TTCACAGACA CACAAGCACC TCCATCACCA
    AACAGGTCTG TCAGTGGGAA GAAACTGTGC TCAAGTTGTG GTCACCCACT TGGCAAAGGT
    GCGGCCATGA TTATAGAGAC TCTCAGCCTT TACTTTCACA TCCAGTGCTT TAAGTGTGGA
    ATCTGTAAGG GTCAGTTGGG TGACACCAGC ACAGGAACAG ATGTGAGGAT CAGAAATGGT
    CTGCTCAACT GCCATCAGTG CTACATCCGC TCCCGCTCTG CTGGTCAACC CACTACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42538
    Clone ID Related Accession (Same CDS sequence) XM_009291003.2 , XM_009291003.3
    Accession Version XM_009291003.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGTC CCTCCCTCAG TGCTGAATCT GGTGAATCGG
    GGTGAACACT TTTCTTGGGA CCCTGAGGAA GAGCGCAATC GTCAGGAGAG GTGGCAGAAA
    GAGCAGGAAC GCATGCTTCA GGAAAAGTAT CGACGTGAGC AGGAGAAGCT AAAGCAGGAG
    TGGGAGCAGG CGCAGAAGGA GGTGGAGGAG GAGGAGAGAC GCTACCATGA GGAGGAGCAG
    AAGATTCTGG AAGAGACTGT GACCCCTTTG ACCCCACACT CACCAATTGC ACCGCCAGTC
    CTTCCCAAAA CTCAAAGCAC TTTACACTCC CAGCCCGATC GGGAATATAA GCAGGGGCCA
    GTGGAGAAGC AGCCGAGTTC TGAGAACTGC AGACAAAGGG AAAACGGCAT CAGTGAAGCA
    GCACAAAGAA AGACGGAGAG TTTACAGATG ACAGTAAGTT CTCCCCTCCA AAGCTGTGGG
    TCAGTTGGTC AGAGAGGACA AGAAGACCAG AGCCAAGATA AGCAGCCAAC ACTACAGTCT
    AGTCAGGATA TGCCACTGGG CAATAGACAG ATACCCCAAC AGAATGAGAC ATGGAAGAAG
    AATACAACAC CACCAAAACC TGGTGGACGC AAGAGGTCCG ACTCTATAGA GAACATTGGG
    CCAAATCCAT CAACATCACC TTCATTCACA GACACACAAG CACCTCCATC ACCAAACAGG
    TCTGTCAGTG GGAAGAAACT GTGCTCAAGT TGTGGTCACC CACTTGGCAA AGGTGCGGCC
    ATGATTATAG AGACTCTCAG CCTTTACTTT CACATCCAGT GCTTTAAGTG TGGAATCTGT
    AAGGGTCAGT TGGGTGACAC CAGCACAGGA ACAGATGTGA GGATCAGAAA TGGTCTGCTC
    AACTGCCATC AGTGCTACAT CCGCTCCCGC TCTGCTGGTC AACCCACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289278.1
    CDS156..3752
    Translation

    Target ORF information:

    RefSeq Version XM_009291003.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGTC CCTCCCTCAG TGCTGAATCT GGTGAATCGG
    GGTGAACACT TTTCTTGGGA CCCTGAGGAA GAGCGCAATC GTCAGGAGAG GTGGCAGAAA
    GAGCAGGAAC GCATGCTTCA GGAAAAGTAT CGACGTGAGC AGGAGAAGCT AAAGCAGGAG
    TGGGAGCAGG CGCAGAAGGA GGTGGAGGAG GAGGAGAGAC GCTACCATGA GGAGGAGCAG
    AAGATTCTGG AAGAGACTGT GACCCCTTTG ACCCCACACT CACCAATTGC ACCGCCAGTC
    CTTCCCAAAA CTCAAAGCAC TTTACACTCC CAGCCCGATC GGGAATATAA GCAGGGGCCA
    GTGGAGAAGC AGCCGAGTTC TGAGAACTGC AGACAAAGGG AAAACGGCAT CAGTGAAGCA
    GCACAAAGAA AGACGGAGAG TTTACAGATG ACAGTAAGTT CTCCCCTCCA AAGCTGTGGG
    TCAGTTGGTC AGAGAGGACA AGAAGACCAG AGCCAAGATA AGCAGCCAAC ACTACAGTCT
    AGTCAGGATA TGCCACTGGG CAATAGACAG ATACCCCAAC AGAATGAGAC ATGGAAGAAG
    AATACAACAC CACCAAAACC TGGTGGACGC AAGAGGTCCG ACTCTATAGA GAACATTGGG
    CCAAATCCAT CAACATCACC TTCATTCACA GACACACAAG CACCTCCATC ACCAAACAGG
    TCTGTCAGTG GGAAGAAACT GTGCTCAAGT TGTGGTCACC CACTTGGCAA AGGTGCGGCC
    ATGATTATAG AGACTCTCAG CCTTTACTTT CACATCCAGT GCTTTAAGTG TGGAATCTGT
    AAGGGTCAGT TGGGTGACAC CAGCACAGGA ACAGATGTGA GGATCAGAAA TGGTCTGCTC
    AACTGCCATC AGTGCTACAT CCGCTCCCGC TCTGCTGGTC AACCCACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42538
    Clone ID Related Accession (Same CDS sequence) XM_009291003.2 , XM_009291003.3
    Accession Version XM_009291003.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGTC CCTCCCTCAG TGCTGAATCT GGTGAATCGG
    GGTGAACACT TTTCTTGGGA CCCTGAGGAA GAGCGCAATC GTCAGGAGAG GTGGCAGAAA
    GAGCAGGAAC GCATGCTTCA GGAAAAGTAT CGACGTGAGC AGGAGAAGCT AAAGCAGGAG
    TGGGAGCAGG CGCAGAAGGA GGTGGAGGAG GAGGAGAGAC GCTACCATGA GGAGGAGCAG
    AAGATTCTGG AAGAGACTGT GACCCCTTTG ACCCCACACT CACCAATTGC ACCGCCAGTC
    CTTCCCAAAA CTCAAAGCAC TTTACACTCC CAGCCCGATC GGGAATATAA GCAGGGGCCA
    GTGGAGAAGC AGCCGAGTTC TGAGAACTGC AGACAAAGGG AAAACGGCAT CAGTGAAGCA
    GCACAAAGAA AGACGGAGAG TTTACAGATG ACAGTAAGTT CTCCCCTCCA AAGCTGTGGG
    TCAGTTGGTC AGAGAGGACA AGAAGACCAG AGCCAAGATA AGCAGCCAAC ACTACAGTCT
    AGTCAGGATA TGCCACTGGG CAATAGACAG ATACCCCAAC AGAATGAGAC ATGGAAGAAG
    AATACAACAC CACCAAAACC TGGTGGACGC AAGAGGTCCG ACTCTATAGA GAACATTGGG
    CCAAATCCAT CAACATCACC TTCATTCACA GACACACAAG CACCTCCATC ACCAAACAGG
    TCTGTCAGTG GGAAGAAACT GTGCTCAAGT TGTGGTCACC CACTTGGCAA AGGTGCGGCC
    ATGATTATAG AGACTCTCAG CCTTTACTTT CACATCCAGT GCTTTAAGTG TGGAATCTGT
    AAGGGTCAGT TGGGTGACAC CAGCACAGGA ACAGATGTGA GGATCAGAAA TGGTCTGCTC
    AACTGCCATC AGTGCTACAT CCGCTCCCGC TCTGCTGGTC AACCCACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289278.1
    CDS163..3759
    Translation

    Target ORF information:

    RefSeq Version XM_009291003.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGTC CCTCCCTCAG TGCTGAATCT GGTGAATCGG
    GGTGAACACT TTTCTTGGGA CCCTGAGGAA GAGCGCAATC GTCAGGAGAG GTGGCAGAAA
    GAGCAGGAAC GCATGCTTCA GGAAAAGTAT CGACGTGAGC AGGAGAAGCT AAAGCAGGAG
    TGGGAGCAGG CGCAGAAGGA GGTGGAGGAG GAGGAGAGAC GCTACCATGA GGAGGAGCAG
    AAGATTCTGG AAGAGACTGT GACCCCTTTG ACCCCACACT CACCAATTGC ACCGCCAGTC
    CTTCCCAAAA CTCAAAGCAC TTTACACTCC CAGCCCGATC GGGAATATAA GCAGGGGCCA
    GTGGAGAAGC AGCCGAGTTC TGAGAACTGC AGACAAAGGG AAAACGGCAT CAGTGAAGCA
    GCACAAAGAA AGACGGAGAG TTTACAGATG ACAGTAAGTT CTCCCCTCCA AAGCTGTGGG
    TCAGTTGGTC AGAGAGGACA AGAAGACCAG AGCCAAGATA AGCAGCCAAC ACTACAGTCT
    AGTCAGGATA TGCCACTGGG CAATAGACAG ATACCCCAAC AGAATGAGAC ATGGAAGAAG
    AATACAACAC CACCAAAACC TGGTGGACGC AAGAGGTCCG ACTCTATAGA GAACATTGGG
    CCAAATCCAT CAACATCACC TTCATTCACA GACACACAAG CACCTCCATC ACCAAACAGG
    TCTGTCAGTG GGAAGAAACT GTGCTCAAGT TGTGGTCACC CACTTGGCAA AGGTGCGGCC
    ATGATTATAG AGACTCTCAG CCTTTACTTT CACATCCAGT GCTTTAAGTG TGGAATCTGT
    AAGGGTCAGT TGGGTGACAC CAGCACAGGA ACAGATGTGA GGATCAGAAA TGGTCTGCTC
    AACTGCCATC AGTGCTACAT CCGCTCCCGC TCTGCTGGTC AACCCACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42539
    Clone ID Related Accession (Same CDS sequence) XM_009291004.2
    Accession Version XM_009291004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGATAT GGCTGAGAAG
    GCTCATGAAA ATGTCCCTCC CTCAGTGCTG AATCTGGTGA ATCGGGGTGA ACACTTTTCT
    TGGGACCCTG AGGAAGAGCG CAATCGTCAG GAGAGGTGGC AGAAAGAGCA GGAACGCATG
    CTTCAGGAAA AGTATCGACG TGAGCAGGAG AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG
    AAGGAGGTGG AGGAGGAGGA GAGACGCTAC CATGAGGAGG AGCAGAAGAT TCTGGAAGAG
    ACTGTGACCC CTTTGACCCC ACACTCACCA ATTGCACCGC CAGTCCTTCC CAAAACTCAA
    AGCACTTTAC ACTCCCAGCC CGATCGGGAA TATAAGCAGG GGCCAGTGGA GAAGCAGCCG
    AGTTCTGAGA ACTGCAGACA AAGGGAAAAC GGCATCAGTG AAGCAGCACA AAGAAAGACG
    GAGAGTTTAC AGATGACAGT AAGTTCTCCC CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA
    GGACAAGAAG ACCAGAGCCA AGATAAGCAG CCAACACTAC AGTCTAGTCA GGATATGCCA
    CTGGGCAATA GACAGATACC CCAACAGAAT GAGACATGGA AGAAGAATAC AACACCACCA
    AAACCTGGTG GACGCAAGAG GTCCGACTCT ATAGAGAACA TTGGGCCAAA TCCATCAACA
    TCACCTTCAT TCACAGACAC ACAAGCACCT CCATCACCAA ACAGGTCTGT CAGTGGGAAG
    AAACTGTGCT CAAGTTGTGG TCACCCACTT GGCAAAGGTG CGGCCATGAT TATAGAGACT
    CTCAGCCTTT ACTTTCACAT CCAGTGCTTT AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT
    GACACCAGCA CAGGAACAGA TGTGAGGATC AGAAATGGTC TGCTCAACTG CCATCAGTGC
    TACATCCGCT CCCGCTCTGC TGGTCAACCC ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289279.1
    CDS156..3677
    Translation

    Target ORF information:

    RefSeq Version XM_009291004.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGATAT GGCTGAGAAG
    GCTCATGAAA ATGTCCCTCC CTCAGTGCTG AATCTGGTGA ATCGGGGTGA ACACTTTTCT
    TGGGACCCTG AGGAAGAGCG CAATCGTCAG GAGAGGTGGC AGAAAGAGCA GGAACGCATG
    CTTCAGGAAA AGTATCGACG TGAGCAGGAG AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG
    AAGGAGGTGG AGGAGGAGGA GAGACGCTAC CATGAGGAGG AGCAGAAGAT TCTGGAAGAG
    ACTGTGACCC CTTTGACCCC ACACTCACCA ATTGCACCGC CAGTCCTTCC CAAAACTCAA
    AGCACTTTAC ACTCCCAGCC CGATCGGGAA TATAAGCAGG GGCCAGTGGA GAAGCAGCCG
    AGTTCTGAGA ACTGCAGACA AAGGGAAAAC GGCATCAGTG AAGCAGCACA AAGAAAGACG
    GAGAGTTTAC AGATGACAGT AAGTTCTCCC CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA
    GGACAAGAAG ACCAGAGCCA AGATAAGCAG CCAACACTAC AGTCTAGTCA GGATATGCCA
    CTGGGCAATA GACAGATACC CCAACAGAAT GAGACATGGA AGAAGAATAC AACACCACCA
    AAACCTGGTG GACGCAAGAG GTCCGACTCT ATAGAGAACA TTGGGCCAAA TCCATCAACA
    TCACCTTCAT TCACAGACAC ACAAGCACCT CCATCACCAA ACAGGTCTGT CAGTGGGAAG
    AAACTGTGCT CAAGTTGTGG TCACCCACTT GGCAAAGGTG CGGCCATGAT TATAGAGACT
    CTCAGCCTTT ACTTTCACAT CCAGTGCTTT AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT
    GACACCAGCA CAGGAACAGA TGTGAGGATC AGAAATGGTC TGCTCAACTG CCATCAGTGC
    TACATCCGCT CCCGCTCTGC TGGTCAACCC ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42540
    Clone ID Related Accession (Same CDS sequence) XM_009291005.2
    Accession Version XM_009291005.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGAA AAGTATCGAC GTGAGCAGGA GAAGCTAAAG
    CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG GAGGAGGAGG AGAGACGCTA CCATGAGGAG
    GAGCAGAAGA TTCTGGAAGA GACTGTGACC CCTTTGACCC CACACTCACC AATTGCACCG
    CCAGTCCTTC CCAAAACTCA AAGCACTTTA CACTCCCAGC CCGATCGGGA ATATAAGCAG
    GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG AACTGCAGAC AAAGGGAAAA CGGCATCAGT
    GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA CAGATGACAG TAAGTTCTCC CCTCCAAAGC
    TGTGGGTCAG TTGGTCAGAG AGGACAAGAA GACCAGAGCC AAGATAAGCA GCCAACACTA
    CAGTCTAGTC AGGATATGCC ACTGGGCAAT AGACAGATAC CCCAACAGAA TGAGACATGG
    AAGAAGAATA CAACACCACC AAAACCTGGT GGACGCAAGA GGTCCGACTC TATAGAGAAC
    ATTGGGCCAA ATCCATCAAC ATCACCTTCA TTCACAGACA CACAAGCACC TCCATCACCA
    AACAGGTCTG TCAGTGGGAA GAAACTGTGC TCAAGTTGTG GTCACCCACT TGGCAAAGGT
    GCGGCCATGA TTATAGAGAC TCTCAGCCTT TACTTTCACA TCCAGTGCTT TAAGTGTGGA
    ATCTGTAAGG GTCAGTTGGG TGACACCAGC ACAGGAACAG ATGTGAGGAT CAGAAATGGT
    CTGCTCAACT GCCATCAGTG CTACATCCGC TCCCGCTCTG CTGGTCAACC CACTACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289280.1
    CDS156..3638
    Translation

    Target ORF information:

    RefSeq Version XM_009291005.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGAGTT GGCTGTCAGT
    GCTCAGTTAA CATCGTTGTC AAGTAATCCT AAATTGCGCA GAGATTTCTT CGCCCAATCA
    GATATGGCTG AGAAGGCTCA TGAAAATGAA AAGTATCGAC GTGAGCAGGA GAAGCTAAAG
    CAGGAGTGGG AGCAGGCGCA GAAGGAGGTG GAGGAGGAGG AGAGACGCTA CCATGAGGAG
    GAGCAGAAGA TTCTGGAAGA GACTGTGACC CCTTTGACCC CACACTCACC AATTGCACCG
    CCAGTCCTTC CCAAAACTCA AAGCACTTTA CACTCCCAGC CCGATCGGGA ATATAAGCAG
    GGGCCAGTGG AGAAGCAGCC GAGTTCTGAG AACTGCAGAC AAAGGGAAAA CGGCATCAGT
    GAAGCAGCAC AAAGAAAGAC GGAGAGTTTA CAGATGACAG TAAGTTCTCC CCTCCAAAGC
    TGTGGGTCAG TTGGTCAGAG AGGACAAGAA GACCAGAGCC AAGATAAGCA GCCAACACTA
    CAGTCTAGTC AGGATATGCC ACTGGGCAAT AGACAGATAC CCCAACAGAA TGAGACATGG
    AAGAAGAATA CAACACCACC AAAACCTGGT GGACGCAAGA GGTCCGACTC TATAGAGAAC
    ATTGGGCCAA ATCCATCAAC ATCACCTTCA TTCACAGACA CACAAGCACC TCCATCACCA
    AACAGGTCTG TCAGTGGGAA GAAACTGTGC TCAAGTTGTG GTCACCCACT TGGCAAAGGT
    GCGGCCATGA TTATAGAGAC TCTCAGCCTT TACTTTCACA TCCAGTGCTT TAAGTGTGGA
    ATCTGTAAGG GTCAGTTGGG TGACACCAGC ACAGGAACAG ATGTGAGGAT CAGAAATGGT
    CTGCTCAACT GCCATCAGTG CTACATCCGC TCCCGCTCTG CTGGTCAACC CACTACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42541
    Clone ID Related Accession (Same CDS sequence) XM_009291006.2
    Accession Version XM_009291006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGATAT GGCTGAGAAG
    GCTCATGAAA ATGAAAAGTA TCGACGTGAG CAGGAGAAGC TAAAGCAGGA GTGGGAGCAG
    GCGCAGAAGG AGGTGGAGGA GGAGGAGAGA CGCTACCATG AGGAGGAGCA GAAGATTCTG
    GAAGAGACTG TGACCCCTTT GACCCCACAC TCACCAATTG CACCGCCAGT CCTTCCCAAA
    ACTCAAAGCA CTTTACACTC CCAGCCCGAT CGGGAATATA AGCAGGGGCC AGTGGAGAAG
    CAGCCGAGTT CTGAGAACTG CAGACAAAGG GAAAACGGCA TCAGTGAAGC AGCACAAAGA
    AAGACGGAGA GTTTACAGAT GACAGTAAGT TCTCCCCTCC AAAGCTGTGG GTCAGTTGGT
    CAGAGAGGAC AAGAAGACCA GAGCCAAGAT AAGCAGCCAA CACTACAGTC TAGTCAGGAT
    ATGCCACTGG GCAATAGACA GATACCCCAA CAGAATGAGA CATGGAAGAA GAATACAACA
    CCACCAAAAC CTGGTGGACG CAAGAGGTCC GACTCTATAG AGAACATTGG GCCAAATCCA
    TCAACATCAC CTTCATTCAC AGACACACAA GCACCTCCAT CACCAAACAG GTCTGTCAGT
    GGGAAGAAAC TGTGCTCAAG TTGTGGTCAC CCACTTGGCA AAGGTGCGGC CATGATTATA
    GAGACTCTCA GCCTTTACTT TCACATCCAG TGCTTTAAGT GTGGAATCTG TAAGGGTCAG
    TTGGGTGACA CCAGCACAGG AACAGATGTG AGGATCAGAA ATGGTCTGCT CAACTGCCAT
    CAGTGCTACA TCCGCTCCCG CTCTGCTGGT CAACCCACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289281.1
    CDS155..3562
    Translation

    Target ORF information:

    RefSeq Version XM_009291006.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGTGAGAGT GAAACCCTTT CGTTAGAGTT GTCCGCTGTG
    GTAACAGGAC GTGATGTTCA CTGTGTAGAT TCACAGCAAA TAGATCCTGA CTCTAAATAC
    TCCAGAGCGT CTCCTCAAAA CGAGTCTCCC CCCAAAGACC CAATTTTTCT TAATCCACAA
    CTCAGTGACC CTCAGATTAG ATCCCCCCTT CCCAAAGACA CCCTCCATCC TTCTGTCCAA
    CCCCAACCTC AAGAGCAGAG CCACTCAGAC CAGGAGAAGA TGGAGGAGGA GGAAAAGGAC
    AGAGATGGAT GGGCCACTTT CTGCCAAAGT CCTCCAAACC CCTTGGATAC CCTTCAGAGC
    GACTTACAGA TGTCCCATAA TCCGACTGCA GGAAATGATA CCAGGAGCGT GAGCATGATT
    GACATGAGAA GCGAGGAAGA CTTGTCACCA CATAGTCAAG TTCGACACGA GCTCATGCAC
    AACCAGTACA ACAAGCTGAA AGAGGAAGAA GACCACTGGC AGGATGATTT GGCCAAATGG
    AAGAGTCGCA GGAGGAGCGT GTCACAGGAC CTGATCAAAA AAGAAGAGGA GAGGAAGATG
    ATGGAGAGAC TGCTAAGTGG AGATGGAGGA TCACAGAGAA GGAAAAGCAT TAAGACGTAC
    AGAGAAATAG TAGAGGAGAA GGAGCGTCGT GAACAGGAGC TGCATGACGC TTATCGAAAG
    GCTCGCACAC CAGAGGAGGC AGCAGCTGTG CTGCATCGCT ACGCCCAGCG ATTCACCATC
    AGCGAGGCTG TTCTGGAGCG CCTCAAACTG CCAAAGCTGC TGGAGAGGAG TGTGTCGGCT
    GATCCCACTC GTCACTCCTC TCTCCCCATC TCCCAGGACA TCTCGTCCTC TTTCAGCGCT
    TCCCCTAATG TGCTGCAGTA CCTAAGGCAG CAGTCTCTTC CCGCAGCTAA GTTCACATCC
    ATGGTCGAGG CAAGGGTGAC GGGATACAGC CCAGAAAAGG GAATTGTGGG AGATGCAGAG
    TGTGTCTCAG ATCCATCTCG CTCACATGGG CCATCACTGG GTCCGAAACC TTACAGACAG
    AGCAGAGCTG CCACTGCTGG GATGAGAACA GTAAAGTTGG ATGAAAGAGT GCGAGTGAAT
    GGGGAAAGGG AAGATGACGG CCTCGGCATT GAGAAGACAG GACCACAGAG AAACAAAGCA
    GAGGAAGAAG ATTCTTCTCA TCTACAGTTC TTCTCATCTT CCTGCCAGCA CACAGACTCA
    GACATGACAG ATAGTGAAGT CTCTGTAGTT CAGCCCACGG TCCACATGCT CTCATCCACA
    CGCACAGCAG AGAGCCCAGA GAAACACAGA GATACTACAG AGATTGAAGG ACGGACGGTC
    CCGCAGGTCG GAGATGTCCC TGAATTCCCA AAAGTGTTGG AGATAAATCC AGATGAGACT
    GTTACAGATC AAGAGAAGAC AGATGAGAGG GGTATGAGTG ACTCTCTACA CGACGTGATT
    CAAATGGAGA TTTCTGCACA GGTCAGTGAA CTAACAGCTG AGATCCCACA GAAATCAACA
    AATGAATCTG AGTCATCCAG ACCCGAATCA TACAGAACCA AAGCAGATAT GGCTGAGAAG
    GCTCATGAAA ATGAAAAGTA TCGACGTGAG CAGGAGAAGC TAAAGCAGGA GTGGGAGCAG
    GCGCAGAAGG AGGTGGAGGA GGAGGAGAGA CGCTACCATG AGGAGGAGCA GAAGATTCTG
    GAAGAGACTG TGACCCCTTT GACCCCACAC TCACCAATTG CACCGCCAGT CCTTCCCAAA
    ACTCAAAGCA CTTTACACTC CCAGCCCGAT CGGGAATATA AGCAGGGGCC AGTGGAGAAG
    CAGCCGAGTT CTGAGAACTG CAGACAAAGG GAAAACGGCA TCAGTGAAGC AGCACAAAGA
    AAGACGGAGA GTTTACAGAT GACAGTAAGT TCTCCCCTCC AAAGCTGTGG GTCAGTTGGT
    CAGAGAGGAC AAGAAGACCA GAGCCAAGAT AAGCAGCCAA CACTACAGTC TAGTCAGGAT
    ATGCCACTGG GCAATAGACA GATACCCCAA CAGAATGAGA CATGGAAGAA GAATACAACA
    CCACCAAAAC CTGGTGGACG CAAGAGGTCC GACTCTATAG AGAACATTGG GCCAAATCCA
    TCAACATCAC CTTCATTCAC AGACACACAA GCACCTCCAT CACCAAACAG GTCTGTCAGT
    GGGAAGAAAC TGTGCTCAAG TTGTGGTCAC CCACTTGGCA AAGGTGCGGC CATGATTATA
    GAGACTCTCA GCCTTTACTT TCACATCCAG TGCTTTAAGT GTGGAATCTG TAAGGGTCAG
    TTGGGTGACA CCAGCACAGG AACAGATGTG AGGATCAGAA ATGGTCTGCT CAACTGCCAT
    CAGTGCTACA TCCGCTCCCG CTCTGCTGGT CAACCCACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42542
    Clone ID Related Accession (Same CDS sequence) XM_009291007.2 , XM_009291007.3
    Accession Version XM_009291007.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GAGTTGGCTG TCAGTGCTCA GTTAACATCG TTGTCAAGTA ATCCTAAATT GCGCAGAGAT
    TTCTTCGCCC AATCAGATAT GGCTGAGAAG GCTCATGAAA ATGTCCCTCC CTCAGTGCTG
    AATCTGGTGA ATCGGGGTGA ACACTTTTCT TGGGACCCTG AGGAAGAGCG CAATCGTCAG
    GAGAGGTGGC AGAAAGAGCA GGAACGCATG CTTCAGGAAA AGTATCGACG TGAGCAGGAG
    AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG AAGGAGGTGG AGGAGGAGGA GAGACGCTAC
    CATGAGGAGG AGCAGAAGAT TCTGGAAGAG ACTGTGACCC CTTTGACCCC ACACTCACCA
    ATTGCACCGC CAGTCCTTCC CAAAACTCAA AGCACTTTAC ACTCCCAGCC CGATCGGGAA
    TATAAGCAGG GGCCAGTGGA GAAGCAGCCG AGTTCTGAGA ACTGCAGACA AAGGGAAAAC
    GGCATCAGTG AAGCAGCACA AAGAAAGACG GAGAGTTTAC AGATGACAGT AAGTTCTCCC
    CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA GGACAAGAAG ACCAGAGCCA AGATAAGCAG
    CCAACACTAC AGTCTAGTCA GGATATGCCA CTGGGCAATA GACAGATACC CCAACAGAAT
    GAGACATGGA AGAAGAATAC AACACCACCA AAACCTGGTG GACGCAAGAG GTCCGACTCT
    ATAGAGAACA TTGGGCCAAA TCCATCAACA TCACCTTCAT TCACAGACAC ACAAGCACCT
    CCATCACCAA ACAGGTCTGT CAGTGGGAAG AAACTGTGCT CAAGTTGTGG TCACCCACTT
    GGCAAAGGTG CGGCCATGAT TATAGAGACT CTCAGCCTTT ACTTTCACAT CCAGTGCTTT
    AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT GACACCAGCA CAGGAACAGA TGTGAGGATC
    AGAAATGGTC TGCTCAACTG CCATCAGTGC TACATCCGCT CCCGCTCTGC TGGTCAACCC
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289282.1
    CDS155..3406
    Translation

    Target ORF information:

    RefSeq Version XM_009291007.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GAGTTGGCTG TCAGTGCTCA GTTAACATCG TTGTCAAGTA ATCCTAAATT GCGCAGAGAT
    TTCTTCGCCC AATCAGATAT GGCTGAGAAG GCTCATGAAA ATGTCCCTCC CTCAGTGCTG
    AATCTGGTGA ATCGGGGTGA ACACTTTTCT TGGGACCCTG AGGAAGAGCG CAATCGTCAG
    GAGAGGTGGC AGAAAGAGCA GGAACGCATG CTTCAGGAAA AGTATCGACG TGAGCAGGAG
    AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG AAGGAGGTGG AGGAGGAGGA GAGACGCTAC
    CATGAGGAGG AGCAGAAGAT TCTGGAAGAG ACTGTGACCC CTTTGACCCC ACACTCACCA
    ATTGCACCGC CAGTCCTTCC CAAAACTCAA AGCACTTTAC ACTCCCAGCC CGATCGGGAA
    TATAAGCAGG GGCCAGTGGA GAAGCAGCCG AGTTCTGAGA ACTGCAGACA AAGGGAAAAC
    GGCATCAGTG AAGCAGCACA AAGAAAGACG GAGAGTTTAC AGATGACAGT AAGTTCTCCC
    CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA GGACAAGAAG ACCAGAGCCA AGATAAGCAG
    CCAACACTAC AGTCTAGTCA GGATATGCCA CTGGGCAATA GACAGATACC CCAACAGAAT
    GAGACATGGA AGAAGAATAC AACACCACCA AAACCTGGTG GACGCAAGAG GTCCGACTCT
    ATAGAGAACA TTGGGCCAAA TCCATCAACA TCACCTTCAT TCACAGACAC ACAAGCACCT
    CCATCACCAA ACAGGTCTGT CAGTGGGAAG AAACTGTGCT CAAGTTGTGG TCACCCACTT
    GGCAAAGGTG CGGCCATGAT TATAGAGACT CTCAGCCTTT ACTTTCACAT CCAGTGCTTT
    AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT GACACCAGCA CAGGAACAGA TGTGAGGATC
    AGAAATGGTC TGCTCAACTG CCATCAGTGC TACATCCGCT CCCGCTCTGC TGGTCAACCC
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42542
    Clone ID Related Accession (Same CDS sequence) XM_009291007.2 , XM_009291007.3
    Accession Version XM_009291007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GAGTTGGCTG TCAGTGCTCA GTTAACATCG TTGTCAAGTA ATCCTAAATT GCGCAGAGAT
    TTCTTCGCCC AATCAGATAT GGCTGAGAAG GCTCATGAAA ATGTCCCTCC CTCAGTGCTG
    AATCTGGTGA ATCGGGGTGA ACACTTTTCT TGGGACCCTG AGGAAGAGCG CAATCGTCAG
    GAGAGGTGGC AGAAAGAGCA GGAACGCATG CTTCAGGAAA AGTATCGACG TGAGCAGGAG
    AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG AAGGAGGTGG AGGAGGAGGA GAGACGCTAC
    CATGAGGAGG AGCAGAAGAT TCTGGAAGAG ACTGTGACCC CTTTGACCCC ACACTCACCA
    ATTGCACCGC CAGTCCTTCC CAAAACTCAA AGCACTTTAC ACTCCCAGCC CGATCGGGAA
    TATAAGCAGG GGCCAGTGGA GAAGCAGCCG AGTTCTGAGA ACTGCAGACA AAGGGAAAAC
    GGCATCAGTG AAGCAGCACA AAGAAAGACG GAGAGTTTAC AGATGACAGT AAGTTCTCCC
    CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA GGACAAGAAG ACCAGAGCCA AGATAAGCAG
    CCAACACTAC AGTCTAGTCA GGATATGCCA CTGGGCAATA GACAGATACC CCAACAGAAT
    GAGACATGGA AGAAGAATAC AACACCACCA AAACCTGGTG GACGCAAGAG GTCCGACTCT
    ATAGAGAACA TTGGGCCAAA TCCATCAACA TCACCTTCAT TCACAGACAC ACAAGCACCT
    CCATCACCAA ACAGGTCTGT CAGTGGGAAG AAACTGTGCT CAAGTTGTGG TCACCCACTT
    GGCAAAGGTG CGGCCATGAT TATAGAGACT CTCAGCCTTT ACTTTCACAT CCAGTGCTTT
    AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT GACACCAGCA CAGGAACAGA TGTGAGGATC
    AGAAATGGTC TGCTCAACTG CCATCAGTGC TACATCCGCT CCCGCTCTGC TGGTCAACCC
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289282.1
    CDS161..3412
    Translation

    Target ORF information:

    RefSeq Version XM_009291007.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGATTCAGC CGTTCATCTA GTGTGTTTGA TGATCCAGAG
    AGCGTGAGCA TGATTGACAT GAGAAGCGAG GAAGACTTGT CACCACATAG TCAAGTTCGA
    CACGAGCTCA TGCACAACCA GTACAACAAG CTGAAAGAGG AAGAAGACCA CTGGCAGGAT
    GATTTGGCCA AATGGAAGAG TCGCAGGAGG AGCGTGTCAC AGGACCTGAT CAAAAAAGAA
    GAGGAGAGGA AGATGATGGA GAGACTGCTA AGTGGAGATG GAGGATCACA GAGAAGGAAA
    AGCATTAAGA CGTACAGAGA AATAGTAGAG GAGAAGGAGC GTCGTGAACA GGAGCTGCAT
    GACGCTTATC GAAAGGCTCG CACACCAGAG GAGGCAGCAG CTGTGCTGCA TCGCTACGCC
    CAGCGATTCA CCATCAGCGA GGCTGTTCTG GAGCGCCTCA AACTGCCAAA GCTGCTGGAG
    AGGAGTGTGT CGGCTGATCC CACTCGTCAC TCCTCTCTCC CCATCTCCCA GGACATCTCG
    TCCTCTTTCA GCGCTTCCCC TAATGTGCTG CAGTACCTAA GGCAGCAGTC TCTTCCCGCA
    GCTAAGTTCA CATCCATGGT CGAGGCAAGG GTGACGGGAT ACAGCCCAGA AAAGGGAATT
    GTGGGAGATG CAGAGTGTGT CTCAGATCCA TCTCGCTCAC ATGGGCCATC ACTGGGTCCG
    AAACCTTACA GACAGAGCAG AGCTGCCACT GCTGGGATGA GAACAGTAAA GTTGGATGAA
    AGAGTGCGAG TGAATGGGGA AAGGGAAGAT GACGGCCTCG GCATTGAGAA GACAGGACCA
    CAGAGAAACA AAGCAGAGGA AGAAGATTCT TCTCATCTAC AGTTCTTCTC ATCTTCCTGC
    CAGCACACAG ACTCAGACAT GACAGATAGT GAAGTCTCTG TAGTTCAGCC CACGGTCCAC
    ATGCTCTCAT CCACACGCAC AGCAGAGAGC CCAGAGAAAC ACAGAGATAC TACAGAGATT
    GAAGGACGGA CGGTCCCGCA GGTCGGAGAT GTCCCTGAAT TCCCAAAAGT GTTGGAGATA
    AATCCAGATG AGACTGTTAC AGATCAAGAG AAGACAGATG AGAGGGGTAT GAGTGACTCT
    CTACACGACG TGATTCAAAT GGAGATTTCT GCACAGGTCA GTGAACTAAC AGCTGAGATC
    CCACAGAAAT CAACAAATGA ATCTGAGTCA TCCAGACCCG AATCATACAG AACCAAAGCA
    GAGTTGGCTG TCAGTGCTCA GTTAACATCG TTGTCAAGTA ATCCTAAATT GCGCAGAGAT
    TTCTTCGCCC AATCAGATAT GGCTGAGAAG GCTCATGAAA ATGTCCCTCC CTCAGTGCTG
    AATCTGGTGA ATCGGGGTGA ACACTTTTCT TGGGACCCTG AGGAAGAGCG CAATCGTCAG
    GAGAGGTGGC AGAAAGAGCA GGAACGCATG CTTCAGGAAA AGTATCGACG TGAGCAGGAG
    AAGCTAAAGC AGGAGTGGGA GCAGGCGCAG AAGGAGGTGG AGGAGGAGGA GAGACGCTAC
    CATGAGGAGG AGCAGAAGAT TCTGGAAGAG ACTGTGACCC CTTTGACCCC ACACTCACCA
    ATTGCACCGC CAGTCCTTCC CAAAACTCAA AGCACTTTAC ACTCCCAGCC CGATCGGGAA
    TATAAGCAGG GGCCAGTGGA GAAGCAGCCG AGTTCTGAGA ACTGCAGACA AAGGGAAAAC
    GGCATCAGTG AAGCAGCACA AAGAAAGACG GAGAGTTTAC AGATGACAGT AAGTTCTCCC
    CTCCAAAGCT GTGGGTCAGT TGGTCAGAGA GGACAAGAAG ACCAGAGCCA AGATAAGCAG
    CCAACACTAC AGTCTAGTCA GGATATGCCA CTGGGCAATA GACAGATACC CCAACAGAAT
    GAGACATGGA AGAAGAATAC AACACCACCA AAACCTGGTG GACGCAAGAG GTCCGACTCT
    ATAGAGAACA TTGGGCCAAA TCCATCAACA TCACCTTCAT TCACAGACAC ACAAGCACCT
    CCATCACCAA ACAGGTCTGT CAGTGGGAAG AAACTGTGCT CAAGTTGTGG TCACCCACTT
    GGCAAAGGTG CGGCCATGAT TATAGAGACT CTCAGCCTTT ACTTTCACAT CCAGTGCTTT
    AAGTGTGGAA TCTGTAAGGG TCAGTTGGGT GACACCAGCA CAGGAACAGA TGTGAGGATC
    AGAAATGGTC TGCTCAACTG CCATCAGTGC TACATCCGCT CCCGCTCTGC TGGTCAACCC
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42543
    Clone ID Related Accession (Same CDS sequence) XM_009291008.2 , XM_009291008.3
    Accession Version XM_009291008.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGGAGCGTG AGCATGATTG ACATGAGAAG CGAGGAAGAC
    TTGTCACCAC ATAGTCAAGT TCGACACGAG CTCATGCACA ACCAGTACAA CAAGCTGAAA
    GAGGAAGAAG ACCACTGGCA GGATGATTTG GCCAAATGGA AGAGTCGCAG GAGGAGCGTG
    TCACAGGACC TGATCAAAAA AGAAGAGGAG AGGAAGATGA TGGAGAGACT GCTAAGTGGA
    GATGGAGGAT CACAGAGAAG GAAAAGCATT AAGACGTACA GAGAAATAGT AGAGGAGAAG
    GAGCGTCGTG AACAGGAGCT GCATGACGCT TATCGAAAGG CTCGCACACC AGAGGAGGCA
    GCAGCTGTGC TGCATCGCTA CGCCCAGCGA TTCACCATCA GCGAGGCTGT TCTGGAGCGC
    CTCAAACTGC CAAAGCTGCT GGAGAGGAGT GTGTCGGCTG ATCCCACTCG TCACTCCTCT
    CTCCCCATCT CCCAGGACAT CTCGTCCTCT TTCAGCGCTT CCCCTAATGT GCTGCAGTAC
    CTAAGGCAGC AGTCTCTTCC CGCAGCTAAG TTCACATCCA TGGTCGAGGC AAGGGTGACG
    GGATACAGCC CAGAAAAGGG AATTGTGGGA GATGCAGAGT GTGTCTCAGA TCCATCTCGC
    TCACATGGGC CATCACTGGG TCCGAAACCT TACAGACAGA GCAGAGCTGC CACTGCTGGG
    ATGAGAACAG TAAAGTTGGA TGAAAGAGTG CGAGTGAATG GGGAAAGGGA AGATGACGGC
    CTCGGCATTG AGAAGACAGG ACCACAGAGA AACAAAGCAG AGGAAGAAGA TTCTTCTCAT
    CTACAGTTCT TCTCATCTTC CTGCCAGCAC ACAGACTCAG ACATGACAGA TAGTGAAGTC
    TCTGTAGTTC AGCCCACGGT CCACATGCTC TCATCCACAC GCACAGCAGA GAGCCCAGAG
    AAACACAGAG ATACTACAGA GATTGAAGGA CGGACGGTCC CGCAGGTCGG AGATGTCCCT
    GAATTCCCAA AAGTGTTGGA GATAAATCCA GATGAGACTG TTACAGATCA AGAGAAGACA
    GATGAGAGGG GTATGAGTGA CTCTCTACAC GACGTGATTC AAATGGAGAT TTCTGCACAG
    GTCAGTGAAC TAACAGCTGA GATCCCACAG AAATCAACAA ATGAATCTGA GTCATCCAGA
    CCCGAATCAT ACAGAACCAA AGCAGAGTTG GCTGTCAGTG CTCAGTTAAC ATCGTTGTCA
    AGTAATCCTA AATTGCGCAG AGATTTCTTC GCCCAATCAG ATATGGCTGA GAAGGCTCAT
    GAAAATGTCC CTCCCTCAGT GCTGAATCTG GTGAATCGGG GTGAACACTT TTCTTGGGAC
    CCTGAGGAAG AGCGCAATCG TCAGGAGAGG TGGCAGAAAG AGCAGGAACG CATGCTTCAG
    GAAAAGTATC GACGTGAGCA GGAGAAGCTA AAGCAGGAGT GGGAGCAGGC GCAGAAGGAG
    GTGGAGGAGG AGGAGAGACG CTACCATGAG GAGGAGCAGA AGATTCTGGA AGAGACTGTG
    ACCCCTTTGA CCCCACACTC ACCAATTGCA CCGCCAGTCC TTCCCAAAAC TCAAAGCACT
    TTACACTCCC AGCCCGATCG GGAATATAAG CAGGGGCCAG TGGAGAAGCA GCCGAGTTCT
    GAGAACTGCA GACAAAGGGA AAACGGCATC AGTGAAGCAG CACAAAGAAA GACGGAGAGT
    TTACAGATGA CAGTAAGTTC TCCCCTCCAA AGCTGTGGGT CAGTTGGTCA GAGAGGACAA
    GAAGACCAGA GCCAAGATAA GCAGCCAACA CTACAGTCTA GTCAGGATAT GCCACTGGGC
    AATAGACAGA TACCCCAACA GAATGAGACA TGGAAGAAGA ATACAACACC ACCAAAACCT
    GGTGGACGCA AGAGGTCCGA CTCTATAGAG AACATTGGGC CAAATCCATC AACATCACCT
    TCATTCACAG ACACACAAGC ACCTCCATCA CCAAACAGGT CTGTCAGTGG GAAGAAACTG
    TGCTCAAGTT GTGGTCACCC ACTTGGCAAA GGTGCGGCCA TGATTATAGA GACTCTCAGC
    CTTTACTTTC ACATCCAGTG CTTTAAGTGT GGAATCTGTA AGGGTCAGTT GGGTGACACC
    AGCACAGGAA CAGATGTGAG GATCAGAAAT GGTCTGCTCA ACTGCCATCA GTGCTACATC
    CGCTCCCGCT CTGCTGGTCA ACCCACTACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289283.1
    CDS154..3369
    Translation

    Target ORF information:

    RefSeq Version XM_009291008.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGGAGCGTG AGCATGATTG ACATGAGAAG CGAGGAAGAC
    TTGTCACCAC ATAGTCAAGT TCGACACGAG CTCATGCACA ACCAGTACAA CAAGCTGAAA
    GAGGAAGAAG ACCACTGGCA GGATGATTTG GCCAAATGGA AGAGTCGCAG GAGGAGCGTG
    TCACAGGACC TGATCAAAAA AGAAGAGGAG AGGAAGATGA TGGAGAGACT GCTAAGTGGA
    GATGGAGGAT CACAGAGAAG GAAAAGCATT AAGACGTACA GAGAAATAGT AGAGGAGAAG
    GAGCGTCGTG AACAGGAGCT GCATGACGCT TATCGAAAGG CTCGCACACC AGAGGAGGCA
    GCAGCTGTGC TGCATCGCTA CGCCCAGCGA TTCACCATCA GCGAGGCTGT TCTGGAGCGC
    CTCAAACTGC CAAAGCTGCT GGAGAGGAGT GTGTCGGCTG ATCCCACTCG TCACTCCTCT
    CTCCCCATCT CCCAGGACAT CTCGTCCTCT TTCAGCGCTT CCCCTAATGT GCTGCAGTAC
    CTAAGGCAGC AGTCTCTTCC CGCAGCTAAG TTCACATCCA TGGTCGAGGC AAGGGTGACG
    GGATACAGCC CAGAAAAGGG AATTGTGGGA GATGCAGAGT GTGTCTCAGA TCCATCTCGC
    TCACATGGGC CATCACTGGG TCCGAAACCT TACAGACAGA GCAGAGCTGC CACTGCTGGG
    ATGAGAACAG TAAAGTTGGA TGAAAGAGTG CGAGTGAATG GGGAAAGGGA AGATGACGGC
    CTCGGCATTG AGAAGACAGG ACCACAGAGA AACAAAGCAG AGGAAGAAGA TTCTTCTCAT
    CTACAGTTCT TCTCATCTTC CTGCCAGCAC ACAGACTCAG ACATGACAGA TAGTGAAGTC
    TCTGTAGTTC AGCCCACGGT CCACATGCTC TCATCCACAC GCACAGCAGA GAGCCCAGAG
    AAACACAGAG ATACTACAGA GATTGAAGGA CGGACGGTCC CGCAGGTCGG AGATGTCCCT
    GAATTCCCAA AAGTGTTGGA GATAAATCCA GATGAGACTG TTACAGATCA AGAGAAGACA
    GATGAGAGGG GTATGAGTGA CTCTCTACAC GACGTGATTC AAATGGAGAT TTCTGCACAG
    GTCAGTGAAC TAACAGCTGA GATCCCACAG AAATCAACAA ATGAATCTGA GTCATCCAGA
    CCCGAATCAT ACAGAACCAA AGCAGAGTTG GCTGTCAGTG CTCAGTTAAC ATCGTTGTCA
    AGTAATCCTA AATTGCGCAG AGATTTCTTC GCCCAATCAG ATATGGCTGA GAAGGCTCAT
    GAAAATGTCC CTCCCTCAGT GCTGAATCTG GTGAATCGGG GTGAACACTT TTCTTGGGAC
    CCTGAGGAAG AGCGCAATCG TCAGGAGAGG TGGCAGAAAG AGCAGGAACG CATGCTTCAG
    GAAAAGTATC GACGTGAGCA GGAGAAGCTA AAGCAGGAGT GGGAGCAGGC GCAGAAGGAG
    GTGGAGGAGG AGGAGAGACG CTACCATGAG GAGGAGCAGA AGATTCTGGA AGAGACTGTG
    ACCCCTTTGA CCCCACACTC ACCAATTGCA CCGCCAGTCC TTCCCAAAAC TCAAAGCACT
    TTACACTCCC AGCCCGATCG GGAATATAAG CAGGGGCCAG TGGAGAAGCA GCCGAGTTCT
    GAGAACTGCA GACAAAGGGA AAACGGCATC AGTGAAGCAG CACAAAGAAA GACGGAGAGT
    TTACAGATGA CAGTAAGTTC TCCCCTCCAA AGCTGTGGGT CAGTTGGTCA GAGAGGACAA
    GAAGACCAGA GCCAAGATAA GCAGCCAACA CTACAGTCTA GTCAGGATAT GCCACTGGGC
    AATAGACAGA TACCCCAACA GAATGAGACA TGGAAGAAGA ATACAACACC ACCAAAACCT
    GGTGGACGCA AGAGGTCCGA CTCTATAGAG AACATTGGGC CAAATCCATC AACATCACCT
    TCATTCACAG ACACACAAGC ACCTCCATCA CCAAACAGGT CTGTCAGTGG GAAGAAACTG
    TGCTCAAGTT GTGGTCACCC ACTTGGCAAA GGTGCGGCCA TGATTATAGA GACTCTCAGC
    CTTTACTTTC ACATCCAGTG CTTTAAGTGT GGAATCTGTA AGGGTCAGTT GGGTGACACC
    AGCACAGGAA CAGATGTGAG GATCAGAAAT GGTCTGCTCA ACTGCCATCA GTGCTACATC
    CGCTCCCGCT CTGCTGGTCA ACCCACTACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42543
    Clone ID Related Accession (Same CDS sequence) XM_009291008.2 , XM_009291008.3
    Accession Version XM_009291008.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGGAGCGTG AGCATGATTG ACATGAGAAG CGAGGAAGAC
    TTGTCACCAC ATAGTCAAGT TCGACACGAG CTCATGCACA ACCAGTACAA CAAGCTGAAA
    GAGGAAGAAG ACCACTGGCA GGATGATTTG GCCAAATGGA AGAGTCGCAG GAGGAGCGTG
    TCACAGGACC TGATCAAAAA AGAAGAGGAG AGGAAGATGA TGGAGAGACT GCTAAGTGGA
    GATGGAGGAT CACAGAGAAG GAAAAGCATT AAGACGTACA GAGAAATAGT AGAGGAGAAG
    GAGCGTCGTG AACAGGAGCT GCATGACGCT TATCGAAAGG CTCGCACACC AGAGGAGGCA
    GCAGCTGTGC TGCATCGCTA CGCCCAGCGA TTCACCATCA GCGAGGCTGT TCTGGAGCGC
    CTCAAACTGC CAAAGCTGCT GGAGAGGAGT GTGTCGGCTG ATCCCACTCG TCACTCCTCT
    CTCCCCATCT CCCAGGACAT CTCGTCCTCT TTCAGCGCTT CCCCTAATGT GCTGCAGTAC
    CTAAGGCAGC AGTCTCTTCC CGCAGCTAAG TTCACATCCA TGGTCGAGGC AAGGGTGACG
    GGATACAGCC CAGAAAAGGG AATTGTGGGA GATGCAGAGT GTGTCTCAGA TCCATCTCGC
    TCACATGGGC CATCACTGGG TCCGAAACCT TACAGACAGA GCAGAGCTGC CACTGCTGGG
    ATGAGAACAG TAAAGTTGGA TGAAAGAGTG CGAGTGAATG GGGAAAGGGA AGATGACGGC
    CTCGGCATTG AGAAGACAGG ACCACAGAGA AACAAAGCAG AGGAAGAAGA TTCTTCTCAT
    CTACAGTTCT TCTCATCTTC CTGCCAGCAC ACAGACTCAG ACATGACAGA TAGTGAAGTC
    TCTGTAGTTC AGCCCACGGT CCACATGCTC TCATCCACAC GCACAGCAGA GAGCCCAGAG
    AAACACAGAG ATACTACAGA GATTGAAGGA CGGACGGTCC CGCAGGTCGG AGATGTCCCT
    GAATTCCCAA AAGTGTTGGA GATAAATCCA GATGAGACTG TTACAGATCA AGAGAAGACA
    GATGAGAGGG GTATGAGTGA CTCTCTACAC GACGTGATTC AAATGGAGAT TTCTGCACAG
    GTCAGTGAAC TAACAGCTGA GATCCCACAG AAATCAACAA ATGAATCTGA GTCATCCAGA
    CCCGAATCAT ACAGAACCAA AGCAGAGTTG GCTGTCAGTG CTCAGTTAAC ATCGTTGTCA
    AGTAATCCTA AATTGCGCAG AGATTTCTTC GCCCAATCAG ATATGGCTGA GAAGGCTCAT
    GAAAATGTCC CTCCCTCAGT GCTGAATCTG GTGAATCGGG GTGAACACTT TTCTTGGGAC
    CCTGAGGAAG AGCGCAATCG TCAGGAGAGG TGGCAGAAAG AGCAGGAACG CATGCTTCAG
    GAAAAGTATC GACGTGAGCA GGAGAAGCTA AAGCAGGAGT GGGAGCAGGC GCAGAAGGAG
    GTGGAGGAGG AGGAGAGACG CTACCATGAG GAGGAGCAGA AGATTCTGGA AGAGACTGTG
    ACCCCTTTGA CCCCACACTC ACCAATTGCA CCGCCAGTCC TTCCCAAAAC TCAAAGCACT
    TTACACTCCC AGCCCGATCG GGAATATAAG CAGGGGCCAG TGGAGAAGCA GCCGAGTTCT
    GAGAACTGCA GACAAAGGGA AAACGGCATC AGTGAAGCAG CACAAAGAAA GACGGAGAGT
    TTACAGATGA CAGTAAGTTC TCCCCTCCAA AGCTGTGGGT CAGTTGGTCA GAGAGGACAA
    GAAGACCAGA GCCAAGATAA GCAGCCAACA CTACAGTCTA GTCAGGATAT GCCACTGGGC
    AATAGACAGA TACCCCAACA GAATGAGACA TGGAAGAAGA ATACAACACC ACCAAAACCT
    GGTGGACGCA AGAGGTCCGA CTCTATAGAG AACATTGGGC CAAATCCATC AACATCACCT
    TCATTCACAG ACACACAAGC ACCTCCATCA CCAAACAGGT CTGTCAGTGG GAAGAAACTG
    TGCTCAAGTT GTGGTCACCC ACTTGGCAAA GGTGCGGCCA TGATTATAGA GACTCTCAGC
    CTTTACTTTC ACATCCAGTG CTTTAAGTGT GGAATCTGTA AGGGTCAGTT GGGTGACACC
    AGCACAGGAA CAGATGTGAG GATCAGAAAT GGTCTGCTCA ACTGCCATCA GTGCTACATC
    CGCTCCCGCT CTGCTGGTCA ACCCACTACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289283.1
    CDS161..3376
    Translation

    Target ORF information:

    RefSeq Version XM_009291008.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291008.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGAGTC CAGCTTCAGA TGCAGGACGG CATCTTCCTC TGCAGGCTCA TCATCACCAC 
    CACCACCAAC ACCATCACCA TCAACATCAT CAGACCGAAC CGGCGCCCGA ACCCGCTGTC
    CGTGAGGCGC AGAGATGGAT AGAGGCAGTG ACCGGCAGGA GTTTTGCTGA CAGGGACTTC
    AGAACAGGAC TTGACAACGG CATCCTACTG TGCGAACTGC TGAGCTCAAT CAGACCGGGT
    CTGGTGAAGA AGATAAACAG ACTGCCCACT CCTGTAGCTG CTCAGGATAA TGTGGTGCTC
    TTCCTGAAGG GTTGTGAGGA GTTGGGTTTG AAGGGCTCGC AGTTGTTTGA TCCTGGAGAT
    CTACAGGACA CCTCCACGCG CACCAACAAA AGTCCAGACA ACAACAAGAA GTTAAAAAAT
    GTTCTGATCA CTATCTACTG GCTGGGCCGA GCAGCCAATA GCAGCGCCAC ATACAACGGG
    CCCACCCTGG ACCTGCACGA GTTTGAGGGA CTGCTGTCAC AGATGAGAAA GGAAGCAGAA
    GAGTGGGACA GCCCGAAGCG TAGCATCAGG GACAGTGGTT ATATAGACTG CTGGGAGTCT
    GAGCGCAGCG ATTCTCTCTC TCCGCCGAGG CACGCGCGAG ACGACTCCTT CGACAGCCTG
    GACTCCATCG GCTCACGCTC CCATCAAACC CCCTCGCCCG ACGGCCTGCT CGCCCGCGGC
    TACAGCGATG GCCGCGGCAG CGACTCGGAG TGCGACACCC CCCAGAGGAA GATACCGGAT
    GTACGCAAGG ATGACATGTT GGTGCGGCGA ACATCCTGCA GTGAGCCACG GACCGCTGTT
    CCTTTCAATC AGTACCTGCC CAACAAAGCC AACCAGGGTT CCTATGTGCC CCCACCAGTG
    CGACGCCCGC GTGCCGACAG AGACGAAAGC CGCAAAAGCT GGAGTAACGC AACCTCACCT
    GTTGGGGGAG AGAGACCTTT CAGGAGCGTG AGCATGATTG ACATGAGAAG CGAGGAAGAC
    TTGTCACCAC ATAGTCAAGT TCGACACGAG CTCATGCACA ACCAGTACAA CAAGCTGAAA
    GAGGAAGAAG ACCACTGGCA GGATGATTTG GCCAAATGGA AGAGTCGCAG GAGGAGCGTG
    TCACAGGACC TGATCAAAAA AGAAGAGGAG AGGAAGATGA TGGAGAGACT GCTAAGTGGA
    GATGGAGGAT CACAGAGAAG GAAAAGCATT AAGACGTACA GAGAAATAGT AGAGGAGAAG
    GAGCGTCGTG AACAGGAGCT GCATGACGCT TATCGAAAGG CTCGCACACC AGAGGAGGCA
    GCAGCTGTGC TGCATCGCTA CGCCCAGCGA TTCACCATCA GCGAGGCTGT TCTGGAGCGC
    CTCAAACTGC CAAAGCTGCT GGAGAGGAGT GTGTCGGCTG ATCCCACTCG TCACTCCTCT
    CTCCCCATCT CCCAGGACAT CTCGTCCTCT TTCAGCGCTT CCCCTAATGT GCTGCAGTAC
    CTAAGGCAGC AGTCTCTTCC CGCAGCTAAG TTCACATCCA TGGTCGAGGC AAGGGTGACG
    GGATACAGCC CAGAAAAGGG AATTGTGGGA GATGCAGAGT GTGTCTCAGA TCCATCTCGC
    TCACATGGGC CATCACTGGG TCCGAAACCT TACAGACAGA GCAGAGCTGC CACTGCTGGG
    ATGAGAACAG TAAAGTTGGA TGAAAGAGTG CGAGTGAATG GGGAAAGGGA AGATGACGGC
    CTCGGCATTG AGAAGACAGG ACCACAGAGA AACAAAGCAG AGGAAGAAGA TTCTTCTCAT
    CTACAGTTCT TCTCATCTTC CTGCCAGCAC ACAGACTCAG ACATGACAGA TAGTGAAGTC
    TCTGTAGTTC AGCCCACGGT CCACATGCTC TCATCCACAC GCACAGCAGA GAGCCCAGAG
    AAACACAGAG ATACTACAGA GATTGAAGGA CGGACGGTCC CGCAGGTCGG AGATGTCCCT
    GAATTCCCAA AAGTGTTGGA GATAAATCCA GATGAGACTG TTACAGATCA AGAGAAGACA
    GATGAGAGGG GTATGAGTGA CTCTCTACAC GACGTGATTC AAATGGAGAT TTCTGCACAG
    GTCAGTGAAC TAACAGCTGA GATCCCACAG AAATCAACAA ATGAATCTGA GTCATCCAGA
    CCCGAATCAT ACAGAACCAA AGCAGAGTTG GCTGTCAGTG CTCAGTTAAC ATCGTTGTCA
    AGTAATCCTA AATTGCGCAG AGATTTCTTC GCCCAATCAG ATATGGCTGA GAAGGCTCAT
    GAAAATGTCC CTCCCTCAGT GCTGAATCTG GTGAATCGGG GTGAACACTT TTCTTGGGAC
    CCTGAGGAAG AGCGCAATCG TCAGGAGAGG TGGCAGAAAG AGCAGGAACG CATGCTTCAG
    GAAAAGTATC GACGTGAGCA GGAGAAGCTA AAGCAGGAGT GGGAGCAGGC GCAGAAGGAG
    GTGGAGGAGG AGGAGAGACG CTACCATGAG GAGGAGCAGA AGATTCTGGA AGAGACTGTG
    ACCCCTTTGA CCCCACACTC ACCAATTGCA CCGCCAGTCC TTCCCAAAAC TCAAAGCACT
    TTACACTCCC AGCCCGATCG GGAATATAAG CAGGGGCCAG TGGAGAAGCA GCCGAGTTCT
    GAGAACTGCA GACAAAGGGA AAACGGCATC AGTGAAGCAG CACAAAGAAA GACGGAGAGT
    TTACAGATGA CAGTAAGTTC TCCCCTCCAA AGCTGTGGGT CAGTTGGTCA GAGAGGACAA
    GAAGACCAGA GCCAAGATAA GCAGCCAACA CTACAGTCTA GTCAGGATAT GCCACTGGGC
    AATAGACAGA TACCCCAACA GAATGAGACA TGGAAGAAGA ATACAACACC ACCAAAACCT
    GGTGGACGCA AGAGGTCCGA CTCTATAGAG AACATTGGGC CAAATCCATC AACATCACCT
    TCATTCACAG ACACACAAGC ACCTCCATCA CCAAACAGGT CTGTCAGTGG GAAGAAACTG
    TGCTCAAGTT GTGGTCACCC ACTTGGCAAA GGTGCGGCCA TGATTATAGA GACTCTCAGC
    CTTTACTTTC ACATCCAGTG CTTTAAGTGT GGAATCTGTA AGGGTCAGTT GGGTGACACC
    AGCACAGGAA CAGATGTGAG GATCAGAAAT GGTCTGCTCA ACTGCCATCA GTGCTACATC
    CGCTCCCGCT CTGCTGGTCA ACCCACTACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42544
    Clone ID Related Accession (Same CDS sequence) XM_009291009.2
    Accession Version XM_009291009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAAAGG AAGCAGAAGA GTGGGACAGC CCGAAGCGTA GCATCAGGGA CAGTGGTTAT 
    ATAGACTGCT GGGAGTCTGA GCGCAGCGAT TCTCTCTCTC CGCCGAGGCA CGCGCGAGAC
    GACTCCTTCG ACAGCCTGGA CTCCATCGGC TCACGCTCCC ATCAAACCCC CTCGCCCGAC
    GGCCTGCTCG CCCGCGGCTA CAGCGATGGC CGCGGCAGCG ACTCGGAGTG CGACACCCCC
    CAGAGGAAGA TACCGGATGT ACGCAAGGAT GACATGTTGG TGCGGCGAAC ATCCTGCAGT
    GAGCCACGGA CCGCTGTTCC TTTCAATCAG TACCTGCCCA ACAAAGCCAA CCAGGGTTCC
    TATGTGCCCC CACCAGTGCG ACGCCCGCGT GCCGACAGAG ACGAAAGCCG CAAAAGCTGG
    AGTAACGCAA CCTCACCTGT TGGGGGAGAG AGACCTTTCA GTGAGAGTGA AACCCTTTCG
    TTAGAGTTGT CCGCTGTGGT AACAGGACGT GATGTTCACT GTGTAGATTC ACAGCAAATA
    GATCCTGACT CTAAATACTC CAGAGCGTCT CCTCAAAACG AGTCTCCCCC CAAAGACCCA
    ATTTTTCTTA ATCCACAACT CAGTGACCCT CAGATTAGAT CCCCCCTTCC CAAAGACACC
    CTCCATCCTT CTGTCCAACC CCAACCTCAA GAGCAGAGCC ACTCAGACCA GGAGAAGATG
    GAGGAGGAGG AAAAGGACAG AGATGGATGG GCCACTTTCT GCCAAAGTCC TCCAAACCCC
    TTGGATACCC TTCAGAGCGA CTTACAGATG TCCCATAATC CGACTGCAGG AAATGATACC
    AGGAGCGTGA GCATGATTGA CATGAGAAGC GAGGAAGACT TGTCACCACA TAGTCAAGTT
    CGACACGAGC TCATGCACAA CCAGTACAAC AAGCTGAAAG AGGAAGAAGA CCACTGGCAG
    GATGATTTGG CCAAATGGAA GAGTCGCAGG AGGAGCGTGT CACAGGACCT GATCAAAAAA
    GAAGAGGAGA GGAAGATGAT GGAGAGACTG CTAAGTGGAG ATGGAGGATC ACAGAGAAGG
    AAAAGCATTA AGACGTACAG AGAAATAGTA GAGGAGAAGG AGCGTCGTGA ACAGGAGCTG
    CATGACGCTT ATCGAAAGGC TCGCACACCA GAGGAGGCAG CAGCTGTGCT GCATCGCTAC
    GCCCAGCGAT TCACCATCAG CGAGGCTGTT CTGGAGCGCC TCAAACTGCC AAAGCTGCTG
    GAGAGGAGTG TGTCGGCTGA TCCCACTCGT CACTCCTCTC TCCCCATCTC CCAGGACATC
    TCGTCCTCTT TCAGCGCTTC CCCTAATGTG CTGCAGTACC TAAGGCAGCA GTCTCTTCCC
    GCAGCTAAGT TCACATCCAT GGTCGAGGCA AGGGTGACGG GATACAGCCC AGAAAAGGGA
    ATTGTGGGAG ATGCAGAGTG TGTCTCAGAT CCATCTCGCT CACATGGGCC ATCACTGGGT
    CCGAAACCTT ACAGACAGAG CAGAGCTGCC ACTGCTGGGA TGAGAACAGT AAAGTTGGAT
    GAAAGAGTGC GAGTGAATGG GGAAAGGGAA GATGACGGCC TCGGCATTGA GAAGACAGGA
    CCACAGAGAA ACAAAGCAGA GGAAGAAGAT TCTTCTCATC TACAGTTCTT CTCATCTTCC
    TGCCAGCACA CAGACTCAGA CATGACAGAT AGTGAAGTCT CTGTAGTTCA GCCCACGGTC
    CACATGCTCT CATCCACACG CACAGCAGAG AGCCCAGAGA AACACAGAGA TACTACAGAG
    ATTGAAGGAC GGACGGTCCC GCAGGTCGGA GATGTCCCTG AATTCCCAAA AGTGTTGGAG
    ATAAATCCAG ATGAGACTGT TACAGATCAA GAGAAGACAG ATGAGAGGGG TATGAGTGAC
    TCTCTACACG ACGTGATTCA AATGGAGATT TCTGCACAGG TCAGTGAACT AACAGCTGAG
    ATCCCACAGA AATCAACAAA TGAATCTGAG TCATCCAGAC CCGAATCATA CAGAACCAAA
    GCAGAGTTGG CTGTCAGTGC TCAGTTAACA TCGTTGTCAA GTAATCCTAA ATTGCGCAGA
    GATTTCTTCG CCCAATCAGA TATGGCTGAG AAGGCTCATG AAAATGTCCC TCCCTCAGTG
    CTGAATCTGG TGAATCGGGG TGAACACTTT TCTTGGGACC CTGAGGAAGA GCGCAATCGT
    CAGGAGAGGT GGCAGAAAGA GCAGGAACGC ATGCTTCAGG AAAAGTATCG ACGTGAGCAG
    GAGAAGCTAA AGCAGGAGTG GGAGCAGGCG CAGAAGGAGG TGGAGGAGGA GGAGAGACGC
    TACCATGAGG AGGAGCAGAA GATTCTGGAA GAGACTGTGA CCCCTTTGAC CCCACACTCA
    CCAATTGCAC CGCCAGTCCT TCCCAAAACT CAAAGCACTT TACACTCCCA GCCCGATCGG
    GAATATAAGC AGGGGCCAGT GGAGAAGCAG CCGAGTTCTG AGAACTGCAG ACAAAGGGAA
    AACGGCATCA GTGAAGCAGC ACAAAGAAAG ACGGAGAGTT TACAGATGAC AGTAAGTTCT
    CCCCTCCAAA GCTGTGGGTC AGTTGGTCAG AGAGGACAAG AAGACCAGAG CCAAGATAAG
    CAGCCAACAC TACAGTCTAG TCAGGATATG CCACTGGGCA ATAGACAGAT ACCCCAACAG
    AATGAGACAT GGAAGAAGAA TACAACACCA CCAAAACCTG GTGGACGCAA GAGGTCCGAC
    TCTATAGAGA ACATTGGGCC AAATCCATCA ACATCACCTT CATTCACAGA CACACAAGCA
    CCTCCATCAC CAAACAGGTC TGTCAGTGGG AAGAAACTGT GCTCAAGTTG TGGTCACCCA
    CTTGGCAAAG GTGCGGCCAT GATTATAGAG ACTCTCAGCC TTTACTTTCA CATCCAGTGC
    TTTAAGTGTG GAATCTGTAA GGGTCAGTTG GGTGACACCA GCACAGGAAC AGATGTGAGG
    ATCAGAAATG GTCTGCTCAA CTGCCATCAG TGCTACATCC GCTCCCGCTC TGCTGGTCAA
    CCCACTACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289284.1
    CDS468..3542
    Translation

    Target ORF information:

    RefSeq Version XM_009291009.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1b (limch1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAAAGG AAGCAGAAGA GTGGGACAGC CCGAAGCGTA GCATCAGGGA CAGTGGTTAT 
    ATAGACTGCT GGGAGTCTGA GCGCAGCGAT TCTCTCTCTC CGCCGAGGCA CGCGCGAGAC
    GACTCCTTCG ACAGCCTGGA CTCCATCGGC TCACGCTCCC ATCAAACCCC CTCGCCCGAC
    GGCCTGCTCG CCCGCGGCTA CAGCGATGGC CGCGGCAGCG ACTCGGAGTG CGACACCCCC
    CAGAGGAAGA TACCGGATGT ACGCAAGGAT GACATGTTGG TGCGGCGAAC ATCCTGCAGT
    GAGCCACGGA CCGCTGTTCC TTTCAATCAG TACCTGCCCA ACAAAGCCAA CCAGGGTTCC
    TATGTGCCCC CACCAGTGCG ACGCCCGCGT GCCGACAGAG ACGAAAGCCG CAAAAGCTGG
    AGTAACGCAA CCTCACCTGT TGGGGGAGAG AGACCTTTCA GTGAGAGTGA AACCCTTTCG
    TTAGAGTTGT CCGCTGTGGT AACAGGACGT GATGTTCACT GTGTAGATTC ACAGCAAATA
    GATCCTGACT CTAAATACTC CAGAGCGTCT CCTCAAAACG AGTCTCCCCC CAAAGACCCA
    ATTTTTCTTA ATCCACAACT CAGTGACCCT CAGATTAGAT CCCCCCTTCC CAAAGACACC
    CTCCATCCTT CTGTCCAACC CCAACCTCAA GAGCAGAGCC ACTCAGACCA GGAGAAGATG
    GAGGAGGAGG AAAAGGACAG AGATGGATGG GCCACTTTCT GCCAAAGTCC TCCAAACCCC
    TTGGATACCC TTCAGAGCGA CTTACAGATG TCCCATAATC CGACTGCAGG AAATGATACC
    AGGAGCGTGA GCATGATTGA CATGAGAAGC GAGGAAGACT TGTCACCACA TAGTCAAGTT
    CGACACGAGC TCATGCACAA CCAGTACAAC AAGCTGAAAG AGGAAGAAGA CCACTGGCAG
    GATGATTTGG CCAAATGGAA GAGTCGCAGG AGGAGCGTGT CACAGGACCT GATCAAAAAA
    GAAGAGGAGA GGAAGATGAT GGAGAGACTG CTAAGTGGAG ATGGAGGATC ACAGAGAAGG
    AAAAGCATTA AGACGTACAG AGAAATAGTA GAGGAGAAGG AGCGTCGTGA ACAGGAGCTG
    CATGACGCTT ATCGAAAGGC TCGCACACCA GAGGAGGCAG CAGCTGTGCT GCATCGCTAC
    GCCCAGCGAT TCACCATCAG CGAGGCTGTT CTGGAGCGCC TCAAACTGCC AAAGCTGCTG
    GAGAGGAGTG TGTCGGCTGA TCCCACTCGT CACTCCTCTC TCCCCATCTC CCAGGACATC
    TCGTCCTCTT TCAGCGCTTC CCCTAATGTG CTGCAGTACC TAAGGCAGCA GTCTCTTCCC
    GCAGCTAAGT TCACATCCAT GGTCGAGGCA AGGGTGACGG GATACAGCCC AGAAAAGGGA
    ATTGTGGGAG ATGCAGAGTG TGTCTCAGAT CCATCTCGCT CACATGGGCC ATCACTGGGT
    CCGAAACCTT ACAGACAGAG CAGAGCTGCC ACTGCTGGGA TGAGAACAGT AAAGTTGGAT
    GAAAGAGTGC GAGTGAATGG GGAAAGGGAA GATGACGGCC TCGGCATTGA GAAGACAGGA
    CCACAGAGAA ACAAAGCAGA GGAAGAAGAT TCTTCTCATC TACAGTTCTT CTCATCTTCC
    TGCCAGCACA CAGACTCAGA CATGACAGAT AGTGAAGTCT CTGTAGTTCA GCCCACGGTC
    CACATGCTCT CATCCACACG CACAGCAGAG AGCCCAGAGA AACACAGAGA TACTACAGAG
    ATTGAAGGAC GGACGGTCCC GCAGGTCGGA GATGTCCCTG AATTCCCAAA AGTGTTGGAG
    ATAAATCCAG ATGAGACTGT TACAGATCAA GAGAAGACAG ATGAGAGGGG TATGAGTGAC
    TCTCTACACG ACGTGATTCA AATGGAGATT TCTGCACAGG TCAGTGAACT AACAGCTGAG
    ATCCCACAGA AATCAACAAA TGAATCTGAG TCATCCAGAC CCGAATCATA CAGAACCAAA
    GCAGAGTTGG CTGTCAGTGC TCAGTTAACA TCGTTGTCAA GTAATCCTAA ATTGCGCAGA
    GATTTCTTCG CCCAATCAGA TATGGCTGAG AAGGCTCATG AAAATGTCCC TCCCTCAGTG
    CTGAATCTGG TGAATCGGGG TGAACACTTT TCTTGGGACC CTGAGGAAGA GCGCAATCGT
    CAGGAGAGGT GGCAGAAAGA GCAGGAACGC ATGCTTCAGG AAAAGTATCG ACGTGAGCAG
    GAGAAGCTAA AGCAGGAGTG GGAGCAGGCG CAGAAGGAGG TGGAGGAGGA GGAGAGACGC
    TACCATGAGG AGGAGCAGAA GATTCTGGAA GAGACTGTGA CCCCTTTGAC CCCACACTCA
    CCAATTGCAC CGCCAGTCCT TCCCAAAACT CAAAGCACTT TACACTCCCA GCCCGATCGG
    GAATATAAGC AGGGGCCAGT GGAGAAGCAG CCGAGTTCTG AGAACTGCAG ACAAAGGGAA
    AACGGCATCA GTGAAGCAGC ACAAAGAAAG ACGGAGAGTT TACAGATGAC AGTAAGTTCT
    CCCCTCCAAA GCTGTGGGTC AGTTGGTCAG AGAGGACAAG AAGACCAGAG CCAAGATAAG
    CAGCCAACAC TACAGTCTAG TCAGGATATG CCACTGGGCA ATAGACAGAT ACCCCAACAG
    AATGAGACAT GGAAGAAGAA TACAACACCA CCAAAACCTG GTGGACGCAA GAGGTCCGAC
    TCTATAGAGA ACATTGGGCC AAATCCATCA ACATCACCTT CATTCACAGA CACACAAGCA
    CCTCCATCAC CAAACAGGTC TGTCAGTGGG AAGAAACTGT GCTCAAGTTG TGGTCACCCA
    CTTGGCAAAG GTGCGGCCAT GATTATAGAG ACTCTCAGCC TTTACTTTCA CATCCAGTGC
    TTTAAGTGTG GAATCTGTAA GGGTCAGTTG GGTGACACCA GCACAGGAAC AGATGTGAGG
    ATCAGAAATG GTCTGCTCAA CTGCCATCAG TGCTACATCC GCTCCCGCTC TGCTGGTCAA
    CCCACTACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.