LIMCH1 cDNA ORF clone, Canis lupus familiaris(dog)

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe292973 XM_022426925.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe292974 XM_022426926.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292975 XM_022426927.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292976 XM_022426928.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292977 XM_022426936.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292977 XM_022426937.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292977 XM_022426929.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292978 XM_022426930.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292979 XM_022426931.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292980 XM_022426932.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292981 XM_022426933.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292982 XM_022426934.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39785 XM_014118774.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39786 XM_014118775.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39787 XM_014118776.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39788 XM_014118777.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39789 XM_014118778.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39790 XM_014118779.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39791 XM_014118780.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39792 XM_014118781.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39793 XM_014118782.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39794 XM_014118783.1
Latest version!
Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe292973
    Clone ID Related Accession (Same CDS sequence) XM_022426925.1
    Accession Version XM_022426925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGCTATCCT GAAACGATAG AGGAAGAGGG AAGTGAAGTG
    GGGTCTCCTT GTGACGAGGA CCCCGCCGGC CAAGTGCAGC CCGGTGGGAA GCCTTGTGGC
    AGGGGCTCAG GACGGCCCGC CGGCCGCGGG ATGGAGCAGC CTCCTCCGGG GGGGCCCACG
    GCGGCACCAG CTCCCCAGTC CGAAGACAGA GATGCCGTCG AAGTCCAGAA GCGCCGACGG
    CTGGAGCAAG CTGGGATCAG AGTGAGGCCG GCGGCGCGGC GCTTTGCCAG TCACAAGCCG
    TCCAGTAGCG GGGAGGAAGC CGTTCAGGAC ACCATCCTTC GCAAAGATAA CCCTTCCCCG
    ACCCACCCGC GAGGCAAAGA TTCAGAGTCA GGAGACAACG TCGCGCGTCG ACGGCCCGAC
    CCTGAGAAAG ATGATTTTGC TGTGAGGAGA GCGAGGATGA ACCAGACCAA GCCAAGGGTG
    CCGTTGAATC AACTCCTCTA CGGGCCGTAC CAGAAGAGAG AGGCGGGAAA ATCAGACGGC
    AGAGAAGAGC ACTCGAAGGG AATTTCAGAA AAGAACAGGC TAGAAGATAA AAGATACGGG
    CCGAGGACTC CTGTGTCTGA TGACGCAGAG AGCTCCAGCC TGCTGGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC ACACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTCATAAAT
    AGCCAGCTGC GCGAGGAGGA CGACAAGTGG CAGGACGACC TGGCTCGTTG GAAGAGCCGT
    AGAAGAAGTG CTTCCCAGGA CTTAATCAAG AAAGAGGAAG AGAGGAAGAA AATGGAGAAG
    TTACTGGCTG GAGAGGATGG GACGAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG GAGCTGCATG AGGCCTATAA GAACGCGCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAG CAGTACATCG AGAGGTTCAC CATCAGCGAG
    GCTGTCCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACGGAGCCA
    AACTTAACCC CCTTCCTGAA CGACCCTAAC CCCATGAAGT ACCTGCGGCG ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACCGTGGAG ACCACCATCG CCCGGACCAG CGTTCTGGAT
    ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA AGCAAGACTG TCCCTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGACGTTCT GAAGACCTTT
    AAGGTAGATG GGAAAGTCAG CATGAATGGA GAAGCAGTTC ACGGTGACAA GGAGAAGGAG
    AGAGAATGTC CCACAGTGGC ACCTGCTCCC TCATTAACCA AACCCCAGAT GTTTGAAGCT
    GTGGCCAGAG TGCACGGGTC CCCAGTGGAG CTCAAACAGG ACAACAATAG CATTGAGATC
    AACATAAAGA AGCCCAGTTC CGTTCCCCAA GAACTGAGAG CAACCACTGA GGAAACTGAA
    CCCAACAGTC AAGAGGATGA AAATGATGGT GGAAAAACAC AGAAAGGAAA TACAGAACTC
    GCCCCATCAG AGCCACAGCA TTTCACGACA ACTGTGACTC GGTCCAGCCC GACTGTGCCC
    TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG AGGAACGATG TGCCACCAGA AGAAGACCAG
    AAGAAACTAG GAAATGAAAT GAGTGGGAAG GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA
    AAGCCAAAAT CACCAGAACC TGAAGCAACC CTGACATATC CATTTCTGGA AAAAATGTCT
    GAAACCAACC AATTACACTT GCCAAATCTC AATTCTCAAG GGTCGCCTGG CGCTGCCGGC
    TTCCCGCTCA GAGTTCAGAA CTCTTGGCGG CGCTCCCAGT TTTTCTCGCA GTCAGTGGAT
    TCTCCAGGCA GTGAAAAGTC TCCTGTAACT ACTCCTTTCA AGTTTTGGGC GTGGGACCCA
    GAAGAGGAGC GCCGGCGACA GGAAAAGTGG CAACAGGAGC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAACAGAA CAAGCTGAAG GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA
    GAGGAAGAGG AACGCAGATA CTACGAGGAG GAGCGCAAAA TAATTGAAGA CACTGTGGTT
    CCATTTACTC TTTCTTCAAG TTCTGCAGAC CAGCTGTCTA CGTCCTCCTC TGTGACCGAG
    GGCACCGGGA CAGTGAGTAA GATGGACTTG GAAAATTGTC GGGAGGAAGA ACAGGAGACA
    AAACAGAAGA AGCCACTCCG GGGGGACGAC AGTGATTTAA TGCCTAAGAC TAGGGAAGAT
    CACCCCCTGG AGGGAAAGGG CAGCCTAACT CAAGGGGCCT TGGCTCCTTC TAAGAATCCT
    GGATCGAAAG GAGTCCATCA GGACCATCAG CTGGATGCAG AAACGGGGGC CCTCCGCTGC
    GGGATGAACT CCCAGCTAGC TCAGGATCCA CCCCAGAATA CGCAGGTATC AAACCCACCA
    ATGCACGTTT TAGAAGATGT GAAGCCAAAA ACGCTCCCTT TGGATAAAAG CATTAACCAT
    CAGATTGAGT CACCGAGTGA GAGACGGAAG AAAAGCCCTC GAGAGAATTT CCAGGCCGGG
    CACTTGTCCC CCTGCTCTCC CACCCCTCCT GGCCAATCAC CAAACAGGTC GATCAGCGGG
    AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC CTGGGGAAGG GAGCGGCCAT GATCATCGAG
    ACGCTGCAGC TCTACTTCCA CATCCAGTGC TTTCGGTGTG GACTCTGCAA AGGACAACTT
    GGGGACGCGG TGAGCGGGAC AGACGTCAGG ATCCGCAGTG GCCTGCTCAA CTGTAACGAC
    TGCTACCTGC GATCCAGAAG TGCCGGCCAG CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282633.1
    CDS96..4160
    Translation

    Target ORF information:

    RefSeq Version XM_022426925.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGCTATCCT GAAACGATAG AGGAAGAGGG AAGTGAAGTG
    GGGTCTCCTT GTGACGAGGA CCCCGCCGGC CAAGTGCAGC CCGGTGGGAA GCCTTGTGGC
    AGGGGCTCAG GACGGCCCGC CGGCCGCGGG ATGGAGCAGC CTCCTCCGGG GGGGCCCACG
    GCGGCACCAG CTCCCCAGTC CGAAGACAGA GATGCCGTCG AAGTCCAGAA GCGCCGACGG
    CTGGAGCAAG CTGGGATCAG AGTGAGGCCG GCGGCGCGGC GCTTTGCCAG TCACAAGCCG
    TCCAGTAGCG GGGAGGAAGC CGTTCAGGAC ACCATCCTTC GCAAAGATAA CCCTTCCCCG
    ACCCACCCGC GAGGCAAAGA TTCAGAGTCA GGAGACAACG TCGCGCGTCG ACGGCCCGAC
    CCTGAGAAAG ATGATTTTGC TGTGAGGAGA GCGAGGATGA ACCAGACCAA GCCAAGGGTG
    CCGTTGAATC AACTCCTCTA CGGGCCGTAC CAGAAGAGAG AGGCGGGAAA ATCAGACGGC
    AGAGAAGAGC ACTCGAAGGG AATTTCAGAA AAGAACAGGC TAGAAGATAA AAGATACGGG
    CCGAGGACTC CTGTGTCTGA TGACGCAGAG AGCTCCAGCC TGCTGGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC ACACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTCATAAAT
    AGCCAGCTGC GCGAGGAGGA CGACAAGTGG CAGGACGACC TGGCTCGTTG GAAGAGCCGT
    AGAAGAAGTG CTTCCCAGGA CTTAATCAAG AAAGAGGAAG AGAGGAAGAA AATGGAGAAG
    TTACTGGCTG GAGAGGATGG GACGAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG GAGCTGCATG AGGCCTATAA GAACGCGCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAG CAGTACATCG AGAGGTTCAC CATCAGCGAG
    GCTGTCCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACGGAGCCA
    AACTTAACCC CCTTCCTGAA CGACCCTAAC CCCATGAAGT ACCTGCGGCG ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACCGTGGAG ACCACCATCG CCCGGACCAG CGTTCTGGAT
    ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA AGCAAGACTG TCCCTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAACTCTC AAGACGTTCT GAAGACCTTT
    AAGGTAGATG GGAAAGTCAG CATGAATGGA GAAGCAGTTC ACGGTGACAA GGAGAAGGAG
    AGAGAATGTC CCACAGTGGC ACCTGCTCCC TCATTAACCA AACCCCAGAT GTTTGAAGCT
    GTGGCCAGAG TGCACGGGTC CCCAGTGGAG CTCAAACAGG ACAACAATAG CATTGAGATC
    AACATAAAGA AGCCCAGTTC CGTTCCCCAA GAACTGAGAG CAACCACTGA GGAAACTGAA
    CCCAACAGTC AAGAGGATGA AAATGATGGT GGAAAAACAC AGAAAGGAAA TACAGAACTC
    GCCCCATCAG AGCCACAGCA TTTCACGACA ACTGTGACTC GGTCCAGCCC GACTGTGCCC
    TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG AGGAACGATG TGCCACCAGA AGAAGACCAG
    AAGAAACTAG GAAATGAAAT GAGTGGGAAG GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA
    AAGCCAAAAT CACCAGAACC TGAAGCAACC CTGACATATC CATTTCTGGA AAAAATGTCT
    GAAACCAACC AATTACACTT GCCAAATCTC AATTCTCAAG GGTCGCCTGG CGCTGCCGGC
    TTCCCGCTCA GAGTTCAGAA CTCTTGGCGG CGCTCCCAGT TTTTCTCGCA GTCAGTGGAT
    TCTCCAGGCA GTGAAAAGTC TCCTGTAACT ACTCCTTTCA AGTTTTGGGC GTGGGACCCA
    GAAGAGGAGC GCCGGCGACA GGAAAAGTGG CAACAGGAGC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAACAGAA CAAGCTGAAG GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA
    GAGGAAGAGG AACGCAGATA CTACGAGGAG GAGCGCAAAA TAATTGAAGA CACTGTGGTT
    CCATTTACTC TTTCTTCAAG TTCTGCAGAC CAGCTGTCTA CGTCCTCCTC TGTGACCGAG
    GGCACCGGGA CAGTGAGTAA GATGGACTTG GAAAATTGTC GGGAGGAAGA ACAGGAGACA
    AAACAGAAGA AGCCACTCCG GGGGGACGAC AGTGATTTAA TGCCTAAGAC TAGGGAAGAT
    CACCCCCTGG AGGGAAAGGG CAGCCTAACT CAAGGGGCCT TGGCTCCTTC TAAGAATCCT
    GGATCGAAAG GAGTCCATCA GGACCATCAG CTGGATGCAG AAACGGGGGC CCTCCGCTGC
    GGGATGAACT CCCAGCTAGC TCAGGATCCA CCCCAGAATA CGCAGGTATC AAACCCACCA
    ATGCACGTTT TAGAAGATGT GAAGCCAAAA ACGCTCCCTT TGGATAAAAG CATTAACCAT
    CAGATTGAGT CACCGAGTGA GAGACGGAAG AAAAGCCCTC GAGAGAATTT CCAGGCCGGG
    CACTTGTCCC CCTGCTCTCC CACCCCTCCT GGCCAATCAC CAAACAGGTC GATCAGCGGG
    AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC CTGGGGAAGG GAGCGGCCAT GATCATCGAG
    ACGCTGCAGC TCTACTTCCA CATCCAGTGC TTTCGGTGTG GACTCTGCAA AGGACAACTT
    GGGGACGCGG TGAGCGGGAC AGACGTCAGG ATCCGCAGTG GCCTGCTCAA CTGTAACGAC
    TGCTACCTGC GATCCAGAAG TGCCGGCCAG CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292974
    Clone ID Related Accession (Same CDS sequence) XM_022426926.1
    Accession Version XM_022426926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTTGTC CCGCCCATGT GCTGGAAGCC CTTCAGCCTC TGCAGCCGGA GCCGCCCTTC 
    GAGCCCGCTT TCTGCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT
    GACAAAGATT TTCGGACAGG GTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC GGGATTGGAC
    AATATCATCT TATTCTTGAG AGGTTGTAAA GAGCTTGGCC TGAGGGAATC TCAACTTTTT
    GACCCTAGTG ACCTACAGGA TACATCCAAC AGAGTGACCG TCAAGAGCCT CGATTACAGT
    AGGAAACTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAATAGCTCT
    GCGTCCTACA GCGGAACGAC ACTGAACCTG AAGGAGTTCG AAGGCTTGCT GGCTCAGATG
    AGAAAGGAGA CCGATGACAT CGAGAGCCCT AAGCGCAGTA TCCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCCGAGCG CAGCGACTCA CTGTCCCCAC CTCGCCACGG CCGAGATGAT
    TCCTTCGACA GCCTGGACTC CTTCGGGTCC CGATCGCGGC AGACGCCCTC GCCAGATGTT
    GTCCTCCGGG GAAGCAGCGA CGGGAGGGGA AGCGACTCTG AGTCTGACCT GCCGCACCGC
    AAGCTGCCGG ACGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGCGAGCCC
    AAGTCGGCGG TGCCGTTCAA CCAGTACCTC CCGAACAAAA GCAACCAGAC GGCCTACGTG
    CCGGCGCCTC TGAGGAGGAG GAAGGCTGAG CGGGAGGAGT ACCGGAAGAG CTGGAGCACC
    GCCACCTCGC CCCTGGGCGG GGACCGGCCC TGCAGCTATC CTGAAACGAT AGAGGAAGAG
    GGAAGTGAAG TGGGGTCTCC TTGTGACGAG GACCCCGCCG GCCAAGTGCA GCCCGGTGGG
    AAGCCTTGTG GCAGGGGCTC AGGACGGCCC GCCGGCCGCG GGATGGAGCA GCCTCCTCCG
    GGGGGGCCCA CGGCGGCACC AGCTCCCCAG TCCGAAGACA GAGATGCCGT CGAAGTCCAG
    AAGCGCCGAC GGCTGGAGCA AGCTGGGATC AGAGTGAGGC CGGCGGCGCG GCGCTTTGCC
    AGTCACAAGC CGTCCAGTAG CGGGGAGGAA GCCGTTCAGG ACACCATCCT TCGCAAAGAT
    AACCCTTCCC CGACCCACCC GCGAGGCAAA GATTCAGAGT CAGGAGACAA CGTCGCGCGT
    CGACGGCCCG ACCCTGAGAA AGATGATTTT GCTGTGAGGA GAGCGAGGAT GAACCAGACC
    AAGCCAAGGG TGCCGTTGAA TCAACTCCTC TACGGGCCGT ACCAGAAGAG AGAGGCGGGA
    AAATCAGACG GCAGAGAAGA GCACTCGAAG GGAATTTCAG AAAAGAACAG GCTAGAAGAT
    AAAAGATACG GGCCGAGGAC TCCTGTGTCT GATGACGCAG AGAGCTCCAG CCTGCTGGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCACACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTCATAA ATAGCCAGCT GCGCGAGGAG GACGACAAGT GGCAGGACGA CCTGGCTCGT
    TGGAAGAGCC GTAGAAGAAG TGCTTCCCAG GACTTAATCA AGAAAGAGGA AGAGAGGAAG
    AAAATGGAGA AGTTACTGGC TGGAGAGGAT GGGACGAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATCGTTCA AGAAAAGGAG CGGAGAGAGA GGGAGCTGCA TGAGGCCTAT
    AAGAACGCGC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AGCAGTACAT CGAGAGGTTC
    ACCATCAGCG AGGCTGTCCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACGGAGC CAAACTTAAC CCCCTTCCTG AACGACCCTA ACCCCATGAA GTACCTGCGG
    CGACAGTCAC TGCCTCCACC CAAATTCACT GCCACCGTGG AGACCACCAT CGCCCGGACC
    AGCGTTCTGG ATACCGGCGT GTCAGCAGGC GGCGGGTCTC CAAGCAAGAC TGTCCCTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGACGTT
    CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGCATGAATG GAGAAGCAGT TCACGGTGAC
    AAGGAGAAGG AGAGAGAATG TCCCACAGTG GCACCTGCTC CCTCATTAAC CAAACCCCAG
    ATGTTTGAAG CTGTGGCCAG AGTGCACGGG TCCCCAGTGG AGCTCAAACA GGACAACAAT
    AGCATTGAGA TCAACATAAA GAAGCCCAGT TCCGTTCCCC AAGAACTGAG AGCAACCACT
    GAGGAAACTG AACCCAACAG TCAAGAGGAT GAAAATGATG GTGGAAAAAC ACAGAAAGGA
    AATACAGAAC TCGCCCCATC AGAGCCACAG CATTTCACGA CAACTGTGAC TCGGTCCAGC
    CCGACTGTGC CCTTTGTAGA GTTTCCCTCC AGCCCCCAGC GGAGGAACGA TGTGCCACCA
    GAAGAAGACC AGAAGAAACT AGGAAATGAA ATGAGTGGGA AGGTGGAGTT GGTGCTGTCC
    CAAAAGGTGG TAAAGCCAAA ATCACCAGAA CCTGAAGCAA CCCTGACATA TCCATTTCTG
    GAAAAAATGT CTGAAACCAA CCAATTACAC TTGCCAAATC TCAATTCTCA AGGGTCGCCT
    GGCGCTGCCG GCTTCCCGCT CAGAGTTCAG AACTCTTGGC GGCGCTCCCA GTTTTTCTCG
    CAGTCAGTGG ATTCTCCAGG CAGTGAAAAG TCTCCTGTAA CTACTCCTTT CAAGTTTTGG
    GCGTGGGACC CAGAAGAGGA GCGCCGGCGA CAGGAAAAGT GGCAACAGGA GCAGGAACGT
    TTGCTCCAGG AGAGATACCA GAAGGAACAG AACAAGCTGA AGGAAGAGTG GGAAAAGGCC
    CAGAAGGAGG TAGAGGAAGA GGAACGCAGA TACTACGAGG AGGAGCGCAA AATAATTGAA
    GACACTGTGG TTCCATTTAC TCTTTCTTCA AGTTCTGCAG ACCAGCTGTC TACGTCCTCC
    TCTGTGACCG AGGGCACCGG GACAGTGAGT AAGATGGACT TGGAAAATTG TCGGGAGGAA
    GAACAGGAGA CAAAACAGAA GAAGCCACTC CGGGGGGACG ACAGTGATTT AATGCCTAAG
    ACTAGGGAAG ATCACCCCCT GGAGGGAAAG GGCAGCCTAA CTCAAGGGGC CTTGGCTCCT
    TCTAAGAATC CTGGATCGAA AGGAGTCCAT CAGGACCATC AGCTGGATGC AGAAACGGGG
    GCCCTCCGCT GCGGGATGAA CTCCCAGCTA GCTCAGGATC CACCCCAGAA TACGCAGGTA
    TCAAACCCAC CAATGCACGT TTTAGAAGAT GTGAAGCCAA AAACGCTCCC TTTGGATAAA
    AGCATTAACC ATCAGATTGA GTCACCGAGT GAGAGACGGA AGAAAAGCCC TCGAGAGAAT
    TTCCAGGCCG GGCACTTGTC CCCCTGCTCT CCCACCCCTC CTGGCCAATC ACCAAACAGG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AAAAAATCCC TTGTGCCCAC CGCCACGGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282634.1
    CDS25..3927
    Translation

    Target ORF information:

    RefSeq Version XM_022426926.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTTGTC CCGCCCATGT GCTGGAAGCC CTTCAGCCTC TGCAGCCGGA GCCGCCCTTC 
    GAGCCCGCTT TCTGCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT
    GACAAAGATT TTCGGACAGG GTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC GGGATTGGAC
    AATATCATCT TATTCTTGAG AGGTTGTAAA GAGCTTGGCC TGAGGGAATC TCAACTTTTT
    GACCCTAGTG ACCTACAGGA TACATCCAAC AGAGTGACCG TCAAGAGCCT CGATTACAGT
    AGGAAACTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAATAGCTCT
    GCGTCCTACA GCGGAACGAC ACTGAACCTG AAGGAGTTCG AAGGCTTGCT GGCTCAGATG
    AGAAAGGAGA CCGATGACAT CGAGAGCCCT AAGCGCAGTA TCCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCCGAGCG CAGCGACTCA CTGTCCCCAC CTCGCCACGG CCGAGATGAT
    TCCTTCGACA GCCTGGACTC CTTCGGGTCC CGATCGCGGC AGACGCCCTC GCCAGATGTT
    GTCCTCCGGG GAAGCAGCGA CGGGAGGGGA AGCGACTCTG AGTCTGACCT GCCGCACCGC
    AAGCTGCCGG ACGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGCGAGCCC
    AAGTCGGCGG TGCCGTTCAA CCAGTACCTC CCGAACAAAA GCAACCAGAC GGCCTACGTG
    CCGGCGCCTC TGAGGAGGAG GAAGGCTGAG CGGGAGGAGT ACCGGAAGAG CTGGAGCACC
    GCCACCTCGC CCCTGGGCGG GGACCGGCCC TGCAGCTATC CTGAAACGAT AGAGGAAGAG
    GGAAGTGAAG TGGGGTCTCC TTGTGACGAG GACCCCGCCG GCCAAGTGCA GCCCGGTGGG
    AAGCCTTGTG GCAGGGGCTC AGGACGGCCC GCCGGCCGCG GGATGGAGCA GCCTCCTCCG
    GGGGGGCCCA CGGCGGCACC AGCTCCCCAG TCCGAAGACA GAGATGCCGT CGAAGTCCAG
    AAGCGCCGAC GGCTGGAGCA AGCTGGGATC AGAGTGAGGC CGGCGGCGCG GCGCTTTGCC
    AGTCACAAGC CGTCCAGTAG CGGGGAGGAA GCCGTTCAGG ACACCATCCT TCGCAAAGAT
    AACCCTTCCC CGACCCACCC GCGAGGCAAA GATTCAGAGT CAGGAGACAA CGTCGCGCGT
    CGACGGCCCG ACCCTGAGAA AGATGATTTT GCTGTGAGGA GAGCGAGGAT GAACCAGACC
    AAGCCAAGGG TGCCGTTGAA TCAACTCCTC TACGGGCCGT ACCAGAAGAG AGAGGCGGGA
    AAATCAGACG GCAGAGAAGA GCACTCGAAG GGAATTTCAG AAAAGAACAG GCTAGAAGAT
    AAAAGATACG GGCCGAGGAC TCCTGTGTCT GATGACGCAG AGAGCTCCAG CCTGCTGGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCACACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTCATAA ATAGCCAGCT GCGCGAGGAG GACGACAAGT GGCAGGACGA CCTGGCTCGT
    TGGAAGAGCC GTAGAAGAAG TGCTTCCCAG GACTTAATCA AGAAAGAGGA AGAGAGGAAG
    AAAATGGAGA AGTTACTGGC TGGAGAGGAT GGGACGAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATCGTTCA AGAAAAGGAG CGGAGAGAGA GGGAGCTGCA TGAGGCCTAT
    AAGAACGCGC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AGCAGTACAT CGAGAGGTTC
    ACCATCAGCG AGGCTGTCCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACGGAGC CAAACTTAAC CCCCTTCCTG AACGACCCTA ACCCCATGAA GTACCTGCGG
    CGACAGTCAC TGCCTCCACC CAAATTCACT GCCACCGTGG AGACCACCAT CGCCCGGACC
    AGCGTTCTGG ATACCGGCGT GTCAGCAGGC GGCGGGTCTC CAAGCAAGAC TGTCCCTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGACGTT
    CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGCATGAATG GAGAAGCAGT TCACGGTGAC
    AAGGAGAAGG AGAGAGAATG TCCCACAGTG GCACCTGCTC CCTCATTAAC CAAACCCCAG
    ATGTTTGAAG CTGTGGCCAG AGTGCACGGG TCCCCAGTGG AGCTCAAACA GGACAACAAT
    AGCATTGAGA TCAACATAAA GAAGCCCAGT TCCGTTCCCC AAGAACTGAG AGCAACCACT
    GAGGAAACTG AACCCAACAG TCAAGAGGAT GAAAATGATG GTGGAAAAAC ACAGAAAGGA
    AATACAGAAC TCGCCCCATC AGAGCCACAG CATTTCACGA CAACTGTGAC TCGGTCCAGC
    CCGACTGTGC CCTTTGTAGA GTTTCCCTCC AGCCCCCAGC GGAGGAACGA TGTGCCACCA
    GAAGAAGACC AGAAGAAACT AGGAAATGAA ATGAGTGGGA AGGTGGAGTT GGTGCTGTCC
    CAAAAGGTGG TAAAGCCAAA ATCACCAGAA CCTGAAGCAA CCCTGACATA TCCATTTCTG
    GAAAAAATGT CTGAAACCAA CCAATTACAC TTGCCAAATC TCAATTCTCA AGGGTCGCCT
    GGCGCTGCCG GCTTCCCGCT CAGAGTTCAG AACTCTTGGC GGCGCTCCCA GTTTTTCTCG
    CAGTCAGTGG ATTCTCCAGG CAGTGAAAAG TCTCCTGTAA CTACTCCTTT CAAGTTTTGG
    GCGTGGGACC CAGAAGAGGA GCGCCGGCGA CAGGAAAAGT GGCAACAGGA GCAGGAACGT
    TTGCTCCAGG AGAGATACCA GAAGGAACAG AACAAGCTGA AGGAAGAGTG GGAAAAGGCC
    CAGAAGGAGG TAGAGGAAGA GGAACGCAGA TACTACGAGG AGGAGCGCAA AATAATTGAA
    GACACTGTGG TTCCATTTAC TCTTTCTTCA AGTTCTGCAG ACCAGCTGTC TACGTCCTCC
    TCTGTGACCG AGGGCACCGG GACAGTGAGT AAGATGGACT TGGAAAATTG TCGGGAGGAA
    GAACAGGAGA CAAAACAGAA GAAGCCACTC CGGGGGGACG ACAGTGATTT AATGCCTAAG
    ACTAGGGAAG ATCACCCCCT GGAGGGAAAG GGCAGCCTAA CTCAAGGGGC CTTGGCTCCT
    TCTAAGAATC CTGGATCGAA AGGAGTCCAT CAGGACCATC AGCTGGATGC AGAAACGGGG
    GCCCTCCGCT GCGGGATGAA CTCCCAGCTA GCTCAGGATC CACCCCAGAA TACGCAGGTA
    TCAAACCCAC CAATGCACGT TTTAGAAGAT GTGAAGCCAA AAACGCTCCC TTTGGATAAA
    AGCATTAACC ATCAGATTGA GTCACCGAGT GAGAGACGGA AGAAAAGCCC TCGAGAGAAT
    TTCCAGGCCG GGCACTTGTC CCCCTGCTCT CCCACCCCTC CTGGCCAATC ACCAAACAGG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AAAAAATCCC TTGTGCCCAC CGCCACGGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292975
    Clone ID Related Accession (Same CDS sequence) XM_022426927.1
    Accession Version XM_022426927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTTCTG CAGTAGCACG AACAGTCATC ACCATAAATA CTCGGGCCTC GGAACGCTAC 
    CAGCGGAGGG CTGTAAACAC ATCAATCACC AGCCGTGCAG AAATTCCGTT CTCAGCTGTC
    ATCTCAGTTT CAGCCGCAAA GATTAAACGC CTGCATTTGA ATTCAAAGCA CCGCACGGAT
    AGGATCCACG GAGACAATAT CATCTTATTC TTGAGAGGTT GTAAAGAGCT TGGCCTGAGG
    GAATCTCAAC TTTTTGACCC TAGTGACCTA CAGGATACAT CCAACAGAGT GACCGTCAAG
    AGCCTCGATT ACAGTAGGAA ACTGAAAAAT GTATTAGTTA CCATTTACTG GCTGGGAAAA
    GCAGCAAATA GCTCTGCGTC CTACAGCGGA ACGACACTGA ACCTGAAGGA GTTCGAAGGC
    TTGCTGGCTC AGATGAGAAA GGAGACCGAT GACATCGAGA GCCCTAAGCG CAGTATCCGG
    GACAGCGGCT ACATAGACTG CTGGGACTCC GAGCGCAGCG ACTCACTGTC CCCACCTCGC
    CACGGCCGAG ATGATTCCTT CGACAGCCTG GACTCCTTCG GGTCCCGATC GCGGCAGACG
    CCCTCGCCAG ATGTTGTCCT CCGGGGAAGC AGCGACGGGA GGGGAAGCGA CTCTGAGTCT
    GACCTGCCGC ACCGCAAGCT GCCGGACGTG AAGAAGGATG ACATGTCTGC GAGGCGGACG
    TCCCACGGCG AGCCCAAGTC GGCGGTGCCG TTCAACCAGT ACCTCCCGAA CAAAAGCAAC
    CAGACGGCCT ACGTGCCGGC GCCTCTGAGG AGGAGGAAGG CTGAGCGGGA GGAGTACCGG
    AAGAGCTGGA GCACCGCCAC CTCGCCCCTG GGCGGGGACC GGCCCTGCAG CTATCCTGAA
    ACGATAGAGG AAGAGGGAAG TGAAGTGGGG TCTCCTTGTG ACGAGGACCC CGCCGGCCAA
    GTGCAGCCCG GTGGGAAGCC TTGTGGCAGG GGCTCAGGAC GGCCCGCCGG CCGCGGGATG
    GAGCAGCCTC CTCCGGGGGG GCCCACGGCG GCACCAGCTC CCCAGTCCGA AGACAGAGAT
    GCCGTCGAAG TCCAGAAGCG CCGACGGCTG GAGCAAGCTG GGATCAGAGT GAGGCCGGCG
    GCGCGGCGCT TTGCCAGTCA CAAGCCGTCC AGTAGCGGGG AGGAAGCCGT TCAGGACACC
    ATCCTTCGCA AAGATAACCC TTCCCCGACC CACCCGCGAG GCAAAGATTC AGAGTCAGGA
    GACAACGTCG CGCGTCGACG GCCCGACCCT GAGAAAGATG ATTTTGCTGT GAGGAGAGCG
    AGGATGAACC AGACCAAGCC AAGGGTGCCG TTGAATCAAC TCCTCTACGG GCCGTACCAG
    AAGAGAGAGG CGGGAAAATC AGACGGCAGA GAAGAGCACT CGAAGGGAAT TTCAGAAAAG
    AACAGGCTAG AAGATAAAAG ATACGGGCCG AGGACTCCTG TGTCTGATGA CGCAGAGAGC
    TCCAGCCTGC TGGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCACA CAGCAGGGCC
    CGCCAGGAGC AGCTGCAGCT CATAAATAGC CAGCTGCGCG AGGAGGACGA CAAGTGGCAG
    GACGACCTGG CTCGTTGGAA GAGCCGTAGA AGAAGTGCTT CCCAGGACTT AATCAAGAAA
    GAGGAAGAGA GGAAGAAAAT GGAGAAGTTA CTGGCTGGAG AGGATGGGAC GAGTGAACGA
    AGGAAAAGCA TCAAAACCTA CAGAGAAATC GTTCAAGAAA AGGAGCGGAG AGAGAGGGAG
    CTGCATGAGG CCTATAAGAA CGCGCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAGCAG
    TACATCGAGA GGTTCACCAT CAGCGAGGCT GTCCTCGAAC GCTTGGAGAT GCCAAAAATT
    CTGGAAAGAA GCCATTCAAC GGAGCCAAAC TTAACCCCCT TCCTGAACGA CCCTAACCCC
    ATGAAGTACC TGCGGCGACA GTCACTGCCT CCACCCAAAT TCACTGCCAC CGTGGAGACC
    ACCATCGCCC GGACCAGCGT TCTGGATACC GGCGTGTCAG CAGGCGGCGG GTCTCCAAGC
    AAGACTGTCC CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA
    AACTCTCAAG ACGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGCAT GAATGGAGAA
    GCAGTTCACG GTGACAAGGA GAAGGAGAGA GAATGTCCCA CAGTGGCACC TGCTCCCTCA
    TTAACCAAAC CCCAGATGTT TGAAGCTGTG GCCAGAGTGC ACGGGTCCCC AGTGGAGCTC
    AAACAGGACA ACAATAGCAT TGAGATCAAC ATAAAGAAGC CCAGTTCCGT TCCCCAAGAA
    CTGAGAGCAA CCACTGAGGA AACTGAACCC AACAGTCAAG AGGATGAAAA TGATGGTGGA
    AAAACACAGA AAGGAAATAC AGAACTCGCC CCATCAGAGC CACAGCATTT CACGACAACT
    GTGACTCGGT CCAGCCCGAC TGTGCCCTTT GTAGAGTTTC CCTCCAGCCC CCAGCGGAGG
    AACGATGTGC CACCAGAAGA AGACCAGAAG AAACTAGGAA ATGAAATGAG TGGGAAGGTG
    GAGTTGGTGC TGTCCCAAAA GGTGGTAAAG CCAAAATCAC CAGAACCTGA AGCAACCCTG
    ACATATCCAT TTCTGGAAAA AATGTCTGAA ACCAACCAAT TACACTTGCC AAATCTCAAT
    TCTCAAGGGT CGCCTGGCGC TGCCGGCTTC CCGCTCAGAG TTCAGAACTC TTGGCGGCGC
    TCCCAGTTTT TCTCGCAGTC AGTGGATTCT CCAGGCAGTG AAAAGTCTCC TGTAACTACT
    CCTTTCAAGT TTTGGGCGTG GGACCCAGAA GAGGAGCGCC GGCGACAGGA AAAGTGGCAA
    CAGGAGCAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AACAGAACAA GCTGAAGGAA
    GAGTGGGAAA AGGCCCAGAA GGAGGTAGAG GAAGAGGAAC GCAGATACTA CGAGGAGGAG
    CGCAAAATAA TTGAAGACAC TGTGGTTCCA TTTACTCTTT CTTCAAGTTC TGCAGACCAG
    CTGTCTACGT CCTCCTCTGT GACCGAGGGC ACCGGGACAG TGAGTAAGAT GGACTTGGAA
    AATTGTCGGG AGGAAGAACA GGAGACAAAA CAGAAGAAGC CACTCCGGGG GGACGACAGT
    GATTTAATGC CTAAGACTAG GGAAGATCAC CCCCTGGAGG GAAAGGGCAG CCTAACTCAA
    GGGGCCTTGG CTCCTTCTAA GAATCCTGGA TCGAAAGGAG TCCATCAGGA CCATCAGCTG
    GATGCAGAAA CGGGGGCCCT CCGCTGCGGG ATGAACTCCC AGCTAGCTCA GGATCCACCC
    CAGAATACGC AGGTATCAAA CCCACCAATG CACGTTTTAG AAGATGTGAA GCCAAAAACG
    CTCCCTTTGG ATAAAAGCAT TAACCATCAG ATTGAGTCAC CGAGTGAGAG ACGGAAGAAA
    AGCCCTCGAG AGAATTTCCA GGCCGGGCAC TTGTCCCCCT GCTCTCCCAC CCCTCCTGGC
    CAATCACCAA ACAGGTCGAT CAGCGGGAAG AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG
    GGGAAGGGAG CGGCCATGAT CATCGAGACG CTGCAGCTCT ACTTCCACAT CCAGTGCTTT
    CGGTGTGGAC TCTGCAAAGG ACAACTTGGG GACGCGGTGA GCGGGACAGA CGTCAGGATC
    CGCAGTGGCC TGCTCAACTG TAACGACTGC TACCTGCGAT CCAGAAAAAA ATCCCTTGTG
    CCCACCGCCA CGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282635.1
    CDS16..3873
    Translation

    Target ORF information:

    RefSeq Version XM_022426927.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTTCTG CAGTAGCACG AACAGTCATC ACCATAAATA CTCGGGCCTC GGAACGCTAC 
    CAGCGGAGGG CTGTAAACAC ATCAATCACC AGCCGTGCAG AAATTCCGTT CTCAGCTGTC
    ATCTCAGTTT CAGCCGCAAA GATTAAACGC CTGCATTTGA ATTCAAAGCA CCGCACGGAT
    AGGATCCACG GAGACAATAT CATCTTATTC TTGAGAGGTT GTAAAGAGCT TGGCCTGAGG
    GAATCTCAAC TTTTTGACCC TAGTGACCTA CAGGATACAT CCAACAGAGT GACCGTCAAG
    AGCCTCGATT ACAGTAGGAA ACTGAAAAAT GTATTAGTTA CCATTTACTG GCTGGGAAAA
    GCAGCAAATA GCTCTGCGTC CTACAGCGGA ACGACACTGA ACCTGAAGGA GTTCGAAGGC
    TTGCTGGCTC AGATGAGAAA GGAGACCGAT GACATCGAGA GCCCTAAGCG CAGTATCCGG
    GACAGCGGCT ACATAGACTG CTGGGACTCC GAGCGCAGCG ACTCACTGTC CCCACCTCGC
    CACGGCCGAG ATGATTCCTT CGACAGCCTG GACTCCTTCG GGTCCCGATC GCGGCAGACG
    CCCTCGCCAG ATGTTGTCCT CCGGGGAAGC AGCGACGGGA GGGGAAGCGA CTCTGAGTCT
    GACCTGCCGC ACCGCAAGCT GCCGGACGTG AAGAAGGATG ACATGTCTGC GAGGCGGACG
    TCCCACGGCG AGCCCAAGTC GGCGGTGCCG TTCAACCAGT ACCTCCCGAA CAAAAGCAAC
    CAGACGGCCT ACGTGCCGGC GCCTCTGAGG AGGAGGAAGG CTGAGCGGGA GGAGTACCGG
    AAGAGCTGGA GCACCGCCAC CTCGCCCCTG GGCGGGGACC GGCCCTGCAG CTATCCTGAA
    ACGATAGAGG AAGAGGGAAG TGAAGTGGGG TCTCCTTGTG ACGAGGACCC CGCCGGCCAA
    GTGCAGCCCG GTGGGAAGCC TTGTGGCAGG GGCTCAGGAC GGCCCGCCGG CCGCGGGATG
    GAGCAGCCTC CTCCGGGGGG GCCCACGGCG GCACCAGCTC CCCAGTCCGA AGACAGAGAT
    GCCGTCGAAG TCCAGAAGCG CCGACGGCTG GAGCAAGCTG GGATCAGAGT GAGGCCGGCG
    GCGCGGCGCT TTGCCAGTCA CAAGCCGTCC AGTAGCGGGG AGGAAGCCGT TCAGGACACC
    ATCCTTCGCA AAGATAACCC TTCCCCGACC CACCCGCGAG GCAAAGATTC AGAGTCAGGA
    GACAACGTCG CGCGTCGACG GCCCGACCCT GAGAAAGATG ATTTTGCTGT GAGGAGAGCG
    AGGATGAACC AGACCAAGCC AAGGGTGCCG TTGAATCAAC TCCTCTACGG GCCGTACCAG
    AAGAGAGAGG CGGGAAAATC AGACGGCAGA GAAGAGCACT CGAAGGGAAT TTCAGAAAAG
    AACAGGCTAG AAGATAAAAG ATACGGGCCG AGGACTCCTG TGTCTGATGA CGCAGAGAGC
    TCCAGCCTGC TGGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCACA CAGCAGGGCC
    CGCCAGGAGC AGCTGCAGCT CATAAATAGC CAGCTGCGCG AGGAGGACGA CAAGTGGCAG
    GACGACCTGG CTCGTTGGAA GAGCCGTAGA AGAAGTGCTT CCCAGGACTT AATCAAGAAA
    GAGGAAGAGA GGAAGAAAAT GGAGAAGTTA CTGGCTGGAG AGGATGGGAC GAGTGAACGA
    AGGAAAAGCA TCAAAACCTA CAGAGAAATC GTTCAAGAAA AGGAGCGGAG AGAGAGGGAG
    CTGCATGAGG CCTATAAGAA CGCGCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAGCAG
    TACATCGAGA GGTTCACCAT CAGCGAGGCT GTCCTCGAAC GCTTGGAGAT GCCAAAAATT
    CTGGAAAGAA GCCATTCAAC GGAGCCAAAC TTAACCCCCT TCCTGAACGA CCCTAACCCC
    ATGAAGTACC TGCGGCGACA GTCACTGCCT CCACCCAAAT TCACTGCCAC CGTGGAGACC
    ACCATCGCCC GGACCAGCGT TCTGGATACC GGCGTGTCAG CAGGCGGCGG GTCTCCAAGC
    AAGACTGTCC CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA
    AACTCTCAAG ACGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGCAT GAATGGAGAA
    GCAGTTCACG GTGACAAGGA GAAGGAGAGA GAATGTCCCA CAGTGGCACC TGCTCCCTCA
    TTAACCAAAC CCCAGATGTT TGAAGCTGTG GCCAGAGTGC ACGGGTCCCC AGTGGAGCTC
    AAACAGGACA ACAATAGCAT TGAGATCAAC ATAAAGAAGC CCAGTTCCGT TCCCCAAGAA
    CTGAGAGCAA CCACTGAGGA AACTGAACCC AACAGTCAAG AGGATGAAAA TGATGGTGGA
    AAAACACAGA AAGGAAATAC AGAACTCGCC CCATCAGAGC CACAGCATTT CACGACAACT
    GTGACTCGGT CCAGCCCGAC TGTGCCCTTT GTAGAGTTTC CCTCCAGCCC CCAGCGGAGG
    AACGATGTGC CACCAGAAGA AGACCAGAAG AAACTAGGAA ATGAAATGAG TGGGAAGGTG
    GAGTTGGTGC TGTCCCAAAA GGTGGTAAAG CCAAAATCAC CAGAACCTGA AGCAACCCTG
    ACATATCCAT TTCTGGAAAA AATGTCTGAA ACCAACCAAT TACACTTGCC AAATCTCAAT
    TCTCAAGGGT CGCCTGGCGC TGCCGGCTTC CCGCTCAGAG TTCAGAACTC TTGGCGGCGC
    TCCCAGTTTT TCTCGCAGTC AGTGGATTCT CCAGGCAGTG AAAAGTCTCC TGTAACTACT
    CCTTTCAAGT TTTGGGCGTG GGACCCAGAA GAGGAGCGCC GGCGACAGGA AAAGTGGCAA
    CAGGAGCAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AACAGAACAA GCTGAAGGAA
    GAGTGGGAAA AGGCCCAGAA GGAGGTAGAG GAAGAGGAAC GCAGATACTA CGAGGAGGAG
    CGCAAAATAA TTGAAGACAC TGTGGTTCCA TTTACTCTTT CTTCAAGTTC TGCAGACCAG
    CTGTCTACGT CCTCCTCTGT GACCGAGGGC ACCGGGACAG TGAGTAAGAT GGACTTGGAA
    AATTGTCGGG AGGAAGAACA GGAGACAAAA CAGAAGAAGC CACTCCGGGG GGACGACAGT
    GATTTAATGC CTAAGACTAG GGAAGATCAC CCCCTGGAGG GAAAGGGCAG CCTAACTCAA
    GGGGCCTTGG CTCCTTCTAA GAATCCTGGA TCGAAAGGAG TCCATCAGGA CCATCAGCTG
    GATGCAGAAA CGGGGGCCCT CCGCTGCGGG ATGAACTCCC AGCTAGCTCA GGATCCACCC
    CAGAATACGC AGGTATCAAA CCCACCAATG CACGTTTTAG AAGATGTGAA GCCAAAAACG
    CTCCCTTTGG ATAAAAGCAT TAACCATCAG ATTGAGTCAC CGAGTGAGAG ACGGAAGAAA
    AGCCCTCGAG AGAATTTCCA GGCCGGGCAC TTGTCCCCCT GCTCTCCCAC CCCTCCTGGC
    CAATCACCAA ACAGGTCGAT CAGCGGGAAG AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG
    GGGAAGGGAG CGGCCATGAT CATCGAGACG CTGCAGCTCT ACTTCCACAT CCAGTGCTTT
    CGGTGTGGAC TCTGCAAAGG ACAACTTGGG GACGCGGTGA GCGGGACAGA CGTCAGGATC
    CGCAGTGGCC TGCTCAACTG TAACGACTGC TACCTGCGAT CCAGAAAAAA ATCCCTTGTG
    CCCACCGCCA CGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292976
    Clone ID Related Accession (Same CDS sequence) XM_022426928.1
    Accession Version XM_022426928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282636.1
    CDS84..3509
    Translation

    Target ORF information:

    RefSeq Version XM_022426928.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292977
    Clone ID Related Accession (Same CDS sequence) XM_022426929.1 , XM_022426936.1 , XM_022426937.1
    Accession Version XM_022426929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282637.1
    CDS392..3817
    Translation

    Target ORF information:

    RefSeq Version XM_022426929.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292977
    Clone ID Related Accession (Same CDS sequence) XM_022426929.1 , XM_022426936.1 , XM_022426937.1
    Accession Version XM_022426936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282644.1
    CDS363..3788
    Translation

    Target ORF information:

    RefSeq Version XM_022426936.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292977
    Clone ID Related Accession (Same CDS sequence) XM_022426929.1 , XM_022426936.1 , XM_022426937.1
    Accession Version XM_022426937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282645.1
    CDS264..3689
    Translation

    Target ORF information:

    RefSeq Version XM_022426937.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGGGTCG
    CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT CAGAACTCTT GGCGGCGCTC CCAGTTTTTC
    TCGCAGTCAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAAAAAAT CCCTTGTGCC CACCGCCACG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292978
    Clone ID Related Accession (Same CDS sequence) XM_022426930.1
    Accession Version XM_022426930.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAGTGCCG GCCAGCCCAC CACCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282638.1
    CDS83..3502
    Translation

    Target ORF information:

    RefSeq Version XM_022426930.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG
    AATTTCCAGG CCGGGCACTT GTCCCCCTGC TCTCCCACCC CTCCTGGCCA ATCACCAAAC
    AGGTCGATCA GCGGGAAGAA GCTGTGCTCG TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG
    GCCATGATCA TCGAGACGCT GCAGCTCTAC TTCCACATCC AGTGCTTTCG GTGTGGACTC
    TGCAAAGGAC AACTTGGGGA CGCGGTGAGC GGGACAGACG TCAGGATCCG CAGTGGCCTG
    CTCAACTGTA ACGACTGCTA CCTGCGATCC AGAAGTGCCG GCCAGCCCAC CACCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292979
    Clone ID Related Accession (Same CDS sequence) XM_022426931.1
    Accession Version XM_022426931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGGAGCTCC AGCCTGCTGG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCACACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTCAT AAATAGCCAG
    CTGCGCGAGG AGGACGACAA GTGGCAGGAC GACCTGGCTC GTTGGAAGAG CCGTAGAAGA
    AGTGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAGAGGA AGAAAATGGA GAAGTTACTG
    GCTGGAGAGG ATGGGACGAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATCGTT
    CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CATGAGGCCT ATAAGAACGC GCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAGCAGTAC ATCGAGAGGT TCACCATCAG CGAGGCTGTC
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACGGA GCCAAACTTA
    ACCCCCTTCC TGAACGACCC TAACCCCATG AAGTACCTGC GGCGACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACCGT GGAGACCACC ATCGCCCGGA CCAGCGTTCT GGATACCGGC
    GTGTCAGCAG GCGGCGGGTC TCCAAGCAAG ACTGTCCCTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAC TCTCAAGACG TTCTGAAGAC CTTTAAGGTA
    GATGGGAAAG TCAGCATGAA TGGAGAAGCA GTTCACGGTG ACAAGGAGAA GGAGAGAGAA
    TGTCCCACAG TGGCACCTGC TCCCTCATTA ACCAAACCCC AGATGTTTGA AGCTGTGGCC
    AGAGTGCACG GGTCCCCAGT GGAGCTCAAA CAGGACAACA ATAGCATTGA GATCAACATA
    AAGAAGCCCA GTTCCGTTCC CCAAGAACTG AGAGCAACCA CTGAGGAAAC TGAACCCAAC
    AGTCAAGAGG ATGAAAATGA TGGTGGAAAA ACACAGAAAG GAAATACAGA ACTCGCCCCA
    TCAGAGCCAC AGCATTTCAC GACAACTGTG ACTCGGTCCA GCCCGACTGT GCCCTTTGTA
    GAGTTTCCCT CCAGCCCCCA GCGGAGGAAC GATGTGCCAC CAGAAGAAGA CCAGAAGAAA
    CTAGGAAATG AAATGAGTGG GAAGGTGGAG TTGGTGCTGT CCCAAAAGGT GGTAAAGCCA
    AAATCACCAG AACCTGAAGC AACCCTGACA TATCCATTTC TGGAAAAAAT GTCTGAAACC
    AACCAATTAC ACTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAGGCAG TGAAAAGTCT
    CCTGTAACTA CTCCTTTCAA GTTTTGGGCG TGGGACCCAG AAGAGGAGCG CCGGCGACAG
    GAAAAGTGGC AACAGGAGCA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAACAGAAC
    AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG AAGGAGGTAG AGGAAGAGGA ACGCAGATAC
    TACGAGGAGG AGCGCAAAAT AATTGAAGAC ACTGTGGTTC CATTTACTCT TTCTTCAAGT
    TCTGCAGACC AGCTGTCTAC GTCCTCCTCT GTGACCGAGG GCACCGGGAC AGTGAGTAAG
    ATGGACTTGG AAAATTGTCG GGAGGAAGAA CAGGAGACAA AACAGAAGAA GCCACTCCGG
    GGGGACGACA GTGATTTAAT GCCTAAGACT AGGGAAGATC ACCCCCTGGA GGGAAAGGGC
    AGCCTAACTC AAGGGGCCTT GGCTCCTTCT AAGAATCCTG GATCGAAAGG AGTCCATCAG
    GACCATCAGC TGGATGCAGA AACGGGGGCC CTCCGCTGCG GGATGAACTC CCAGCTAGCT
    CAGGATCCAC CCCAGAATAC GCAGGTATCA AACCCACCAA TGCACGTTTT AGAAGATGTG
    AAGCCAAAAA CGCTCCCTTT GGATAAAAGC ATTAACCATC AGATTGAGTC ACCGAGTGAG
    AGACGGAAGA AAAGCCCTCG AGAGAATTTC CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC
    ACCCCTCCTG GCCAATCACC AAACAGGTCG ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT
    GGGCTCCCCC TGGGGAAGGG AGCGGCCATG ATCATCGAGA CGCTGCAGCT CTACTTCCAC
    ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA GGACAACTTG GGGACGCGGT GAGCGGGACA
    GACGTCAGGA TCCGCAGTGG CCTGCTCAAC TGTAACGACT GCTACCTGCG ATCCAGAAGT
    GCCGGCCAGC CCACCACCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282639.1
    CDS82..3465
    Translation

    Target ORF information:

    RefSeq Version XM_022426931.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGGAGCTCC AGCCTGCTGG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCACACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTCAT AAATAGCCAG
    CTGCGCGAGG AGGACGACAA GTGGCAGGAC GACCTGGCTC GTTGGAAGAG CCGTAGAAGA
    AGTGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAGAGGA AGAAAATGGA GAAGTTACTG
    GCTGGAGAGG ATGGGACGAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATCGTT
    CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CATGAGGCCT ATAAGAACGC GCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAGCAGTAC ATCGAGAGGT TCACCATCAG CGAGGCTGTC
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACGGA GCCAAACTTA
    ACCCCCTTCC TGAACGACCC TAACCCCATG AAGTACCTGC GGCGACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACCGT GGAGACCACC ATCGCCCGGA CCAGCGTTCT GGATACCGGC
    GTGTCAGCAG GCGGCGGGTC TCCAAGCAAG ACTGTCCCTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAC TCTCAAGACG TTCTGAAGAC CTTTAAGGTA
    GATGGGAAAG TCAGCATGAA TGGAGAAGCA GTTCACGGTG ACAAGGAGAA GGAGAGAGAA
    TGTCCCACAG TGGCACCTGC TCCCTCATTA ACCAAACCCC AGATGTTTGA AGCTGTGGCC
    AGAGTGCACG GGTCCCCAGT GGAGCTCAAA CAGGACAACA ATAGCATTGA GATCAACATA
    AAGAAGCCCA GTTCCGTTCC CCAAGAACTG AGAGCAACCA CTGAGGAAAC TGAACCCAAC
    AGTCAAGAGG ATGAAAATGA TGGTGGAAAA ACACAGAAAG GAAATACAGA ACTCGCCCCA
    TCAGAGCCAC AGCATTTCAC GACAACTGTG ACTCGGTCCA GCCCGACTGT GCCCTTTGTA
    GAGTTTCCCT CCAGCCCCCA GCGGAGGAAC GATGTGCCAC CAGAAGAAGA CCAGAAGAAA
    CTAGGAAATG AAATGAGTGG GAAGGTGGAG TTGGTGCTGT CCCAAAAGGT GGTAAAGCCA
    AAATCACCAG AACCTGAAGC AACCCTGACA TATCCATTTC TGGAAAAAAT GTCTGAAACC
    AACCAATTAC ACTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAGGCAG TGAAAAGTCT
    CCTGTAACTA CTCCTTTCAA GTTTTGGGCG TGGGACCCAG AAGAGGAGCG CCGGCGACAG
    GAAAAGTGGC AACAGGAGCA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAACAGAAC
    AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG AAGGAGGTAG AGGAAGAGGA ACGCAGATAC
    TACGAGGAGG AGCGCAAAAT AATTGAAGAC ACTGTGGTTC CATTTACTCT TTCTTCAAGT
    TCTGCAGACC AGCTGTCTAC GTCCTCCTCT GTGACCGAGG GCACCGGGAC AGTGAGTAAG
    ATGGACTTGG AAAATTGTCG GGAGGAAGAA CAGGAGACAA AACAGAAGAA GCCACTCCGG
    GGGGACGACA GTGATTTAAT GCCTAAGACT AGGGAAGATC ACCCCCTGGA GGGAAAGGGC
    AGCCTAACTC AAGGGGCCTT GGCTCCTTCT AAGAATCCTG GATCGAAAGG AGTCCATCAG
    GACCATCAGC TGGATGCAGA AACGGGGGCC CTCCGCTGCG GGATGAACTC CCAGCTAGCT
    CAGGATCCAC CCCAGAATAC GCAGGTATCA AACCCACCAA TGCACGTTTT AGAAGATGTG
    AAGCCAAAAA CGCTCCCTTT GGATAAAAGC ATTAACCATC AGATTGAGTC ACCGAGTGAG
    AGACGGAAGA AAAGCCCTCG AGAGAATTTC CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC
    ACCCCTCCTG GCCAATCACC AAACAGGTCG ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT
    GGGCTCCCCC TGGGGAAGGG AGCGGCCATG ATCATCGAGA CGCTGCAGCT CTACTTCCAC
    ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA GGACAACTTG GGGACGCGGT GAGCGGGACA
    GACGTCAGGA TCCGCAGTGG CCTGCTCAAC TGTAACGACT GCTACCTGCG ATCCAGAAGT
    GCCGGCCAGC CCACCACCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292980
    Clone ID Related Accession (Same CDS sequence) XM_022426932.1
    Accession Version XM_022426932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGTCGAT CAGCGGGAAG
    AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG GGGAAGGGAG CGGCCATGAT CATCGAGACG
    CTGCAGCTCT ACTTCCACAT CCAGTGCTTT CGGTGTGGAC TCTGCAAAGG ACAACTTGGG
    GACGCGGTGA GCGGGACAGA CGTCAGGATC CGCAGTGGCC TGCTCAACTG TAACGACTGC
    TACCTGCGAT CCAGAAGTGC CGGCCAGCCC ACCACCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282640.1
    CDS81..3422
    Translation

    Target ORF information:

    RefSeq Version XM_022426932.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGATACGGG CCGAGGACTC CTGTGTCTGA TGACGCAGAG
    AGCTCCAGCC TGCTGGACAT GCGGTGTGAG GAGGAGGCCG CGGTGCAGCC ACACAGCAGG
    GCCCGCCAGG AGCAGCTGCA GCTCATAAAT AGCCAGCTGC GCGAGGAGGA CGACAAGTGG
    CAGGACGACC TGGCTCGTTG GAAGAGCCGT AGAAGAAGTG CTTCCCAGGA CTTAATCAAG
    AAAGAGGAAG AGAGGAAGAA AATGGAGAAG TTACTGGCTG GAGAGGATGG GACGAGTGAA
    CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATCGTTCAAG AAAAGGAGCG GAGAGAGAGG
    GAGCTGCATG AGGCCTATAA GAACGCGCGG TCCCAGGAGG AGGCAGAGGG GATCCTTCAG
    CAGTACATCG AGAGGTTCAC CATCAGCGAG GCTGTCCTCG AACGCTTGGA GATGCCAAAA
    ATTCTGGAAA GAAGCCATTC AACGGAGCCA AACTTAACCC CCTTCCTGAA CGACCCTAAC
    CCCATGAAGT ACCTGCGGCG ACAGTCACTG CCTCCACCCA AATTCACTGC CACCGTGGAG
    ACCACCATCG CCCGGACCAG CGTTCTGGAT ACCGGCGTGT CAGCAGGCGG CGGGTCTCCA
    AGCAAGACTG TCCCTCCCAA AGCAGTGCCT ATGCTGACAC CCAAGCCTTA CTCCCAGCCC
    AAAAACTCTC AAGACGTTCT GAAGACCTTT AAGGTAGATG GGAAAGTCAG CATGAATGGA
    GAAGCAGTTC ACGGTGACAA GGAGAAGGAG AGAGAATGTC CCACAGTGGC ACCTGCTCCC
    TCATTAACCA AACCCCAGAT GTTTGAAGCT GTGGCCAGAG TGCACGGGTC CCCAGTGGAG
    CTCAAACAGG ACAACAATAG CATTGAGATC AACATAAAGA AGCCCAGTTC CGTTCCCCAA
    GAACTGAGAG CAACCACTGA GGAAACTGAA CCCAACAGTC AAGAGGATGA AAATGATGGT
    GGAAAAACAC AGAAAGGAAA TACAGAACTC GCCCCATCAG AGCCACAGCA TTTCACGACA
    ACTGTGACTC GGTCCAGCCC GACTGTGCCC TTTGTAGAGT TTCCCTCCAG CCCCCAGCGG
    AGGAACGATG TGCCACCAGA AGAAGACCAG AAGAAACTAG GAAATGAAAT GAGTGGGAAG
    GTGGAGTTGG TGCTGTCCCA AAAGGTGGTA AAGCCAAAAT CACCAGAACC TGAAGCAACC
    CTGACATATC CATTTCTGGA AAAAATGTCT GAAACCAACC AATTACACTT GCCAAATCTC
    AATTCTCAAG TGGATTCTCC AGGCAGTGAA AAGTCTCCTG TAACTACTCC TTTCAAGTTT
    TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG CGACAGGAAA AGTGGCAACA GGAGCAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAA CAGAACAAGC TGAAGGAAGA GTGGGAAAAG
    GCCCAGAAGG AGGTAGAGGA AGAGGAACGC AGATACTACG AGGAGGAGCG CAAAATAATT
    GAAGACACTG TGGTTCCATT TACTCTTTCT TCAAGTTCTG CAGACCAGCT GTCTACGTCC
    TCCTCTGTGA CCGAGGGCAC CGGGACAGTG AGTAAGATGG ACTTGGAAAA TTGTCGGGAG
    GAAGAACAGG AGACAAAACA GAAGAAGCCA CTCCGGGGGG ACGACAGTGA TTTAATGCCT
    AAGACTAGGG AAGATCACCC CCTGGAGGGA AAGGGCAGCC TAACTCAAGG GGCCTTGGCT
    CCTTCTAAGA ATCCTGGATC GAAAGGAGTC CATCAGGACC ATCAGCTGGA TGCAGAAACG
    GGGGCCCTCC GCTGCGGGAT GAACTCCCAG CTAGCTCAGG ATCCACCCCA GAATACGCAG
    GTATCAAACC CACCAATGCA CGTTTTAGAA GATGTGAAGC CAAAAACGCT CCCTTTGGAT
    AAAAGCATTA ACCATCAGAT TGAGTCACCG AGTGAGAGAC GGAAGTCGAT CAGCGGGAAG
    AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG GGGAAGGGAG CGGCCATGAT CATCGAGACG
    CTGCAGCTCT ACTTCCACAT CCAGTGCTTT CGGTGTGGAC TCTGCAAAGG ACAACTTGGG
    GACGCGGTGA GCGGGACAGA CGTCAGGATC CGCAGTGGCC TGCTCAACTG TAACGACTGC
    TACCTGCGAT CCAGAAGTGC CGGCCAGCCC ACCACCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292981
    Clone ID Related Accession (Same CDS sequence) XM_022426933.1
    Accession Version XM_022426933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGAGGCC TTAGTTGGGA TCCAGGGGAG AAGCAAGTAA CCGGCAGAAG TTTTGGTGAC 
    AAAGATTTTC GGACAGGGTT AGAAAATGGA ATCCTCCTCT GCGAGTTGCT GAATGCTATA
    AAGCCAGGAC TTGTTAAAAA GATCAATAGA TTGCCTACCC CCATTGCGGG ATTGGACAAT
    ATCATCTTAT TCTTGAGAGG TTGTAAAGAG CTTGGCCTGA GGGAATCTCA ACTTTTTGAC
    CCTAGTGACC TACAGGATAC ATCCAACAGA GTGACCGTCA AGAGCCTCGA TTACAGTAGG
    AAACTGAAAA ATGTATTAGT TACCATTTAC TGGCTGGGAA AAGCAGCAAA TAGCTCTGCG
    TCCTACAGCG GAACGACACT GAACCTGAAG GAGTTCGAAG GCTTGCTGGC TCAGATGAGA
    AAGGAGACCG ATGACATCGA GAGCCCTAAG CGCAGTATCC GGGACAGCGG CTACATAGAC
    TGCTGGGACT CCGAGCGCAG CGACTCACTG TCCCCACCTC GCCACGGCCG AGATGATTCC
    TTCGACAGCC TGGACTCCTT CGGGTCCCGA TCGCGGCAGA CGCCCTCGCC AGATGTTGTC
    CTCCGGGGAA GCAGCGACGG GAGGGGAAGC GACTCTGAGT CTGACCTGCC GCACCGCAAG
    CTGCCGGACG TGAAGAAGGA TGACATGTCT GCGAGGCGGA CGTCCCACGG CGAGCCCAAG
    TCGGCGGTGC CGTTCAACCA GTACCTCCCG AACAAAAGCA ACCAGACGGC CTACGTGCCG
    GCGCCTCTGA GGAGGAGGAA GGCTGAGCGG GAGGAGTACC GGAAGAGCTG GAGCACCGCC
    ACCTCGCCCC TGGGCGGGGA CCGGCCCTGC AGCTATCCTG AAACGATAGA GGAAGAGGGA
    AGTGAAGTGG GGTCTCCTTG TGACGAGGAC CCCGCCGGCC AAGTGCAGCC CGGTGGGAAG
    CCTTGTGGCA GGGGCTCAGG ACGGCCCGCC GGCCGCGGGA TGGAGCAGCC TCCTCCGGGG
    GGGCCCACGG CGGCACCAGC TCCCCAGTCC GAAGACAGAG ATGCCGTCGA AGTCCAGAAG
    CGCCGACGGC TGGAGCAAGC TGGGATCAGA GTGAGGCCGG CGGCGCGGCG CTTTGCCAGT
    CACAAGCCGT CCAGTAGCGG GGAGGAAGCC GTTCAGGACA CCATCCTTCG CAAAGATAAC
    CCTTCCCCGA CCCACCCGCG AGGCAAAGAT TCAGAGTCAG GAGACAACGT CGCGCGTCGA
    CGGCCCGACC CTGAGAAAGA TGATTTTGCT GTGAGGAGAG CGAGGATGAA CCAGACCAAG
    CCAAGGGTGC CGTTGAATCA ACTCCTCTAC GGGCCGTACC AGAAGAGAGA GGCGGGAAAA
    TCAGACGGCA GAGAAGAGCA CTCGAAGGGA ATTTCAGAAA AGAACAGGCT AGAAGATAAA
    AGATACGGGC CGAGGACTCC TGTGTCTGAT GACGCAGAGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGA AAAGCCCTCG AGAGAATTTC
    CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC ACCCCTCCTG GCCAATCACC AAACAGGTCG
    ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT GGGCTCCCCC TGGGGAAGGG AGCGGCCATG
    ATCATCGAGA CGCTGCAGCT CTACTTCCAC ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA
    GGACAACTTG GGGACGCGGT GAGCGGGACA GACGTCAGGA TCCGCAGTGG CCTGCTCAAC
    TGTAACGACT GCTACCTGCG ATCCAGAAAA AAATCCCTTG TGCCCACCGC CACGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282641.1
    CDS88..3927
    Translation

    Target ORF information:

    RefSeq Version XM_022426933.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGAGGCC TTAGTTGGGA TCCAGGGGAG AAGCAAGTAA CCGGCAGAAG TTTTGGTGAC 
    AAAGATTTTC GGACAGGGTT AGAAAATGGA ATCCTCCTCT GCGAGTTGCT GAATGCTATA
    AAGCCAGGAC TTGTTAAAAA GATCAATAGA TTGCCTACCC CCATTGCGGG ATTGGACAAT
    ATCATCTTAT TCTTGAGAGG TTGTAAAGAG CTTGGCCTGA GGGAATCTCA ACTTTTTGAC
    CCTAGTGACC TACAGGATAC ATCCAACAGA GTGACCGTCA AGAGCCTCGA TTACAGTAGG
    AAACTGAAAA ATGTATTAGT TACCATTTAC TGGCTGGGAA AAGCAGCAAA TAGCTCTGCG
    TCCTACAGCG GAACGACACT GAACCTGAAG GAGTTCGAAG GCTTGCTGGC TCAGATGAGA
    AAGGAGACCG ATGACATCGA GAGCCCTAAG CGCAGTATCC GGGACAGCGG CTACATAGAC
    TGCTGGGACT CCGAGCGCAG CGACTCACTG TCCCCACCTC GCCACGGCCG AGATGATTCC
    TTCGACAGCC TGGACTCCTT CGGGTCCCGA TCGCGGCAGA CGCCCTCGCC AGATGTTGTC
    CTCCGGGGAA GCAGCGACGG GAGGGGAAGC GACTCTGAGT CTGACCTGCC GCACCGCAAG
    CTGCCGGACG TGAAGAAGGA TGACATGTCT GCGAGGCGGA CGTCCCACGG CGAGCCCAAG
    TCGGCGGTGC CGTTCAACCA GTACCTCCCG AACAAAAGCA ACCAGACGGC CTACGTGCCG
    GCGCCTCTGA GGAGGAGGAA GGCTGAGCGG GAGGAGTACC GGAAGAGCTG GAGCACCGCC
    ACCTCGCCCC TGGGCGGGGA CCGGCCCTGC AGCTATCCTG AAACGATAGA GGAAGAGGGA
    AGTGAAGTGG GGTCTCCTTG TGACGAGGAC CCCGCCGGCC AAGTGCAGCC CGGTGGGAAG
    CCTTGTGGCA GGGGCTCAGG ACGGCCCGCC GGCCGCGGGA TGGAGCAGCC TCCTCCGGGG
    GGGCCCACGG CGGCACCAGC TCCCCAGTCC GAAGACAGAG ATGCCGTCGA AGTCCAGAAG
    CGCCGACGGC TGGAGCAAGC TGGGATCAGA GTGAGGCCGG CGGCGCGGCG CTTTGCCAGT
    CACAAGCCGT CCAGTAGCGG GGAGGAAGCC GTTCAGGACA CCATCCTTCG CAAAGATAAC
    CCTTCCCCGA CCCACCCGCG AGGCAAAGAT TCAGAGTCAG GAGACAACGT CGCGCGTCGA
    CGGCCCGACC CTGAGAAAGA TGATTTTGCT GTGAGGAGAG CGAGGATGAA CCAGACCAAG
    CCAAGGGTGC CGTTGAATCA ACTCCTCTAC GGGCCGTACC AGAAGAGAGA GGCGGGAAAA
    TCAGACGGCA GAGAAGAGCA CTCGAAGGGA ATTTCAGAAA AGAACAGGCT AGAAGATAAA
    AGATACGGGC CGAGGACTCC TGTGTCTGAT GACGCAGAGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGA AAAGCCCTCG AGAGAATTTC
    CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC ACCCCTCCTG GCCAATCACC AAACAGGTCG
    ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT GGGCTCCCCC TGGGGAAGGG AGCGGCCATG
    ATCATCGAGA CGCTGCAGCT CTACTTCCAC ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA
    GGACAACTTG GGGACGCGGT GAGCGGGACA GACGTCAGGA TCCGCAGTGG CCTGCTCAAC
    TGTAACGACT GCTACCTGCG ATCCAGAAAA AAATCCCTTG TGCCCACCGC CACGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292982
    Clone ID Related Accession (Same CDS sequence) XM_022426934.1
    Accession Version XM_022426934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGGAGCTCC AGCCTGCTGG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCACACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTCAT AAATAGCCAG
    CTGCGCGAGG AGGACGACAA GTGGCAGGAC GACCTGGCTC GTTGGAAGAG CCGTAGAAGA
    AGTGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAGAGGA AGAAAATGGA GAAGTTACTG
    GCTGGAGAGG ATGGGACGAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATCGTT
    CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CATGAGGCCT ATAAGAACGC GCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAGCAGTAC ATCGAGAGGT TCACCATCAG CGAGGCTGTC
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACGGA GCCAAACTTA
    ACCCCCTTCC TGAACGACCC TAACCCCATG AAGTACCTGC GGCGACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACCGT GGAGACCACC ATCGCCCGGA CCAGCGTTCT GGATACCGGC
    GTGTCAGCAG GCGGCGGGTC TCCAAGCAAG ACTGTCCCTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAC TCTCAAGACG TTCTGAAGAC CTTTAAGGTA
    GATGGGAAAG TCAGCATGAA TGGAGAAGCA GTTCACGGTG ACAAGGAGAA GGAGAGAGAA
    TGTCCCACAG TGGCACCTGC TCCCTCATTA ACCAAACCCC AGATGTTTGA AGCTGTGGCC
    AGAGTGCACG GGTCCCCAGT GGAGCTCAAA CAGGACAACA ATAGCATTGA GATCAACATA
    AAGAAGCCCA GTTCCGTTCC CCAAGAACTG AGAGCAACCA CTGAGGAAAC TGAACCCAAC
    AGTCAAGAGG ATGAAAATGA TGGTGGAAAA ACACAGAAAG GAAATACAGA ACTCGCCCCA
    TCAGAGCCAC AGCATTTCAC GACAACTGTG ACTCGGTCCA GCCCGACTGT GCCCTTTGTA
    GAGTTTCCCT CCAGCCCCCA GCGGAGGAAC GATGTGCCAC CAGAAGAAGA CCAGAAGAAA
    CTAGGAAATG AAATGAGTGG GAAGGTGGAG TTGGTGCTGT CCCAAAAGGT GGTAAAGCCA
    AAATCACCAG AACCTGAAGC AACCCTGACA TATCCATTTC TGGAAAAAAT GTCTGAAACC
    AACCAATTAC ACTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAGGCAG TGAAAAGTCT
    CCTGTAACTA CTCCTTTCAA GTTTTGGGCG TGGGACCCAG AAGAGGAGCG CCGGCGACAG
    GAAAAGTGGC AACAGGAGCA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAACAGAAC
    AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG AAGGAGGTAG AGGAAGAGGA ACGCAGATAC
    TACGAGGAGG AGCGCAAAAT AATTGAAGAC ACTGTGGTTC CATTTACTCT TTCTTCAAGT
    TCTGCAGACC AGCTGTCTAC GTCCTCCTCT GTGACCGAGG GCACCGGGAC AGTGAGTAAG
    ATGGACTTGG AAAATTGTCG GGAGGAAGAA CAGGAGACAA AACAGAAGAA GCCACTCCGG
    GGGGACGACA GTGATTTAAT GCCTAAGACT AGGGAAGATC ACCCCCTGGA GGGAAAGGGC
    AGCCTAACTC AAGGGGCCTT GGCTCCTTCT AAGAATCCTG GATCGAAAGG AGTCCATCAG
    GACCATCAGC TGGATGCAGA AACGGGGGCC CTCCGCTGCG GGATGAACTC CCAGCTAGCT
    CAGGATCCAC CCCAGAATAC GCAGGTATCA AACCCACCAA TGCACGTTTT AGAAGATGTG
    AAGCCAAAAA CGCTCCCTTT GGATAAAAGC ATTAACCATC AGATTGAGTC ACCGAGTGAG
    AGACGGAAGT CGATCAGCGG GAAGAAGCTG TGCTCGTCCT GTGGGCTCCC CCTGGGGAAG
    GGAGCGGCCA TGATCATCGA GACGCTGCAG CTCTACTTCC ACATCCAGTG CTTTCGGTGT
    GGACTCTGCA AAGGACAACT TGGGGACGCG GTGAGCGGGA CAGACGTCAG GATCCGCAGT
    GGCCTGCTCA ACTGTAACGA CTGCTACCTG CGATCCAGAA GTGCCGGCCA GCCCACCACC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282642.1
    CDS81..3386
    Translation

    Target ORF information:

    RefSeq Version XM_022426934.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTGGAT CTCCGTGTTT TGTGCAGACT TTACCGTCTT TCACTCACGG GCTCAGACAT 
    ACAGCTGTAA TCAGGTGCCT GAAGAGGTGC TCCATCAACA TCACTCATCC GCAGGGAAAT
    GCAACTCAGA ACCGCAGTGA GACATCACGT CACACCCGTT GGGATGGCGA TGGCACCGCC
    CTGGAGAGCG GAAGCAGCGT TGCACTGTCC ACGCTGGAGG AGATACGGGA ACAATCCAGC
    TGCCCCCCAT CAATGGATAA AGAGCAAGTA ACCGGCAGAA GTTTTGGTGA CAAAGATTTT
    CGGACAGGGT TAGAAAATGG AATCCTCCTC TGCGAGTTGC TGAATGCTAT AAAGCCAGGA
    CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCGG GATTGGACAA TATCATCTTA
    TTCTTGAGAG GTTGTAAAGA GCTTGGCCTG AGGGAATCTC AACTTTTTGA CCCTAGTGAC
    CTACAGGATA CATCCAACAG AGTGACCGTC AAGAGCCTCG ATTACAGTAG GAAACTGAAA
    AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA ATAGCTCTGC GTCCTACAGC
    GGAACGACAC TGAACCTGAA GGAGTTCGAA GGCTTGCTGG CTCAGATGAG AAAGGAGACC
    GATGACATCG AGAGCCCTAA GCGCAGTATC CGGGACAGCG GCTACATAGA CTGCTGGGAC
    TCCGAGCGCA GCGACTCACT GTCCCCACCT CGCCACGGCC GAGATGATTC CTTCGACAGC
    CTGGACTCCT TCGGGTCCCG ATCGCGGCAG ACGCCCTCGC CAGATGTTGT CCTCCGGGGA
    AGCAGCGACG GGAGGGGAAG CGACTCTGAG TCTGACCTGC CGCACCGCAA GCTGCCGGAC
    GTGAAGAAGG ATGACATGTC TGCGAGGCGG ACGTCCCACG GCGAGCCCAA GTCGGCGGTG
    CCGTTCAACC AGTACCTCCC GAACAAAAGC AACCAGACGG CCTACGTGCC GGCGCCTCTG
    AGGAGGAGGA AGGCTGAGCG GGAGGAGTAC CGGAAGAGCT GGAGCACCGC CACCTCGCCC
    CTGGGCGGGG ACCGGCCCTG CAGGAGCTCC AGCCTGCTGG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCACACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTCAT AAATAGCCAG
    CTGCGCGAGG AGGACGACAA GTGGCAGGAC GACCTGGCTC GTTGGAAGAG CCGTAGAAGA
    AGTGCTTCCC AGGACTTAAT CAAGAAAGAG GAAGAGAGGA AGAAAATGGA GAAGTTACTG
    GCTGGAGAGG ATGGGACGAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATCGTT
    CAAGAAAAGG AGCGGAGAGA GAGGGAGCTG CATGAGGCCT ATAAGAACGC GCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAGCAGTAC ATCGAGAGGT TCACCATCAG CGAGGCTGTC
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACGGA GCCAAACTTA
    ACCCCCTTCC TGAACGACCC TAACCCCATG AAGTACCTGC GGCGACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACCGT GGAGACCACC ATCGCCCGGA CCAGCGTTCT GGATACCGGC
    GTGTCAGCAG GCGGCGGGTC TCCAAGCAAG ACTGTCCCTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAC TCTCAAGACG TTCTGAAGAC CTTTAAGGTA
    GATGGGAAAG TCAGCATGAA TGGAGAAGCA GTTCACGGTG ACAAGGAGAA GGAGAGAGAA
    TGTCCCACAG TGGCACCTGC TCCCTCATTA ACCAAACCCC AGATGTTTGA AGCTGTGGCC
    AGAGTGCACG GGTCCCCAGT GGAGCTCAAA CAGGACAACA ATAGCATTGA GATCAACATA
    AAGAAGCCCA GTTCCGTTCC CCAAGAACTG AGAGCAACCA CTGAGGAAAC TGAACCCAAC
    AGTCAAGAGG ATGAAAATGA TGGTGGAAAA ACACAGAAAG GAAATACAGA ACTCGCCCCA
    TCAGAGCCAC AGCATTTCAC GACAACTGTG ACTCGGTCCA GCCCGACTGT GCCCTTTGTA
    GAGTTTCCCT CCAGCCCCCA GCGGAGGAAC GATGTGCCAC CAGAAGAAGA CCAGAAGAAA
    CTAGGAAATG AAATGAGTGG GAAGGTGGAG TTGGTGCTGT CCCAAAAGGT GGTAAAGCCA
    AAATCACCAG AACCTGAAGC AACCCTGACA TATCCATTTC TGGAAAAAAT GTCTGAAACC
    AACCAATTAC ACTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAGGCAG TGAAAAGTCT
    CCTGTAACTA CTCCTTTCAA GTTTTGGGCG TGGGACCCAG AAGAGGAGCG CCGGCGACAG
    GAAAAGTGGC AACAGGAGCA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAACAGAAC
    AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG AAGGAGGTAG AGGAAGAGGA ACGCAGATAC
    TACGAGGAGG AGCGCAAAAT AATTGAAGAC ACTGTGGTTC CATTTACTCT TTCTTCAAGT
    TCTGCAGACC AGCTGTCTAC GTCCTCCTCT GTGACCGAGG GCACCGGGAC AGTGAGTAAG
    ATGGACTTGG AAAATTGTCG GGAGGAAGAA CAGGAGACAA AACAGAAGAA GCCACTCCGG
    GGGGACGACA GTGATTTAAT GCCTAAGACT AGGGAAGATC ACCCCCTGGA GGGAAAGGGC
    AGCCTAACTC AAGGGGCCTT GGCTCCTTCT AAGAATCCTG GATCGAAAGG AGTCCATCAG
    GACCATCAGC TGGATGCAGA AACGGGGGCC CTCCGCTGCG GGATGAACTC CCAGCTAGCT
    CAGGATCCAC CCCAGAATAC GCAGGTATCA AACCCACCAA TGCACGTTTT AGAAGATGTG
    AAGCCAAAAA CGCTCCCTTT GGATAAAAGC ATTAACCATC AGATTGAGTC ACCGAGTGAG
    AGACGGAAGT CGATCAGCGG GAAGAAGCTG TGCTCGTCCT GTGGGCTCCC CCTGGGGAAG
    GGAGCGGCCA TGATCATCGA GACGCTGCAG CTCTACTTCC ACATCCAGTG CTTTCGGTGT
    GGACTCTGCA AAGGACAACT TGGGGACGCG GTGAGCGGGA CAGACGTCAG GATCCGCAGT
    GGCCTGCTCA ACTGTAACGA CTGCTACCTG CGATCCAGAA GTGCCGGCCA GCCCACCACC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39785
    Clone ID Related Accession (Same CDS sequence) XM_014118774.1
    Accession Version XM_014118774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTC CCGCCCATGT GCTGGAAGCC CTTCAGCCTC TGCAGCCGGA GCCGCCCTTC 
    GAGCCCGCTT TCTGCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT
    GACAAAGATT TTCGGACAGG GTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC GGGATTGGAC
    AATATCATCT TATTCTTGAG AGGTTGTAAA GAGCTTGGCC TGAGGGAATC TCAACTTTTT
    GACCCTAGTG ACCTACAGGA TACATCCAAC AGAGTGACCG TCAAGAGCCT CGATTACAGT
    AGGAAACTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAATAGCTCT
    GCGTCCTACA GCGGAACGAC ACTGAACCTG AAGGAGTTCG AAGGCTTGCT GGCTCAGATG
    AGAAAGGAGA CCGATGACAT CGAGAGCCCT AAGCGCAGTA TCCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCCGAGCG CAGCGACTCA CTGTCCCCAC CTCGCCACGG CCGAGATGAT
    TCCTTCGACA GCCTGGACTC CTTCGGGTCC CGATCGCGGC AGACGCCCTC GCCAGATGTT
    GTCCTCCGGG GAAGCAGCGA CGGGAGGGGA AGCGACTCTG AGTCTGACCT GCCGCACCGC
    AAGCTGCCGG ACGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGCGAGCCC
    AAGTCGGCGG TGCCGTTCAA CCAGTACCTC CCGAACAAAA GCAACCAGAC GGCCTACGTG
    CCGGCGCCTC TGAGGAGGAG GAAGGCTGAG CGGGAGGAGT ACCGGAAGAG CTGGAGCACC
    GCCACCTCGC CCCTGGGCGG GGACCGGCCC TGCAGCTATC CTGAAACGAT AGAGGAAGAG
    GGAAGTGAAG TGGGGTCTCC TTGTGACGAG GACCCCGCCG GCCAAGTGCA GCCCGGTGGG
    AAGCCTTGTG GCAGGGGCTC AGGACGGCCC GCCGGCCGCG GGATGGAGCA GCCTCCTCCG
    GGGGGGCCCA CGGCGGCACC AGCTCCCCAG TCCGAAGACA GAGATGCCGT CGAAGTCCAG
    AAGCGCCGAC GGCTGGAGCA AGCTGGGATC AGAGTGAGGC CGGCGGCGCG GCGCTTTGCC
    AGTCACAAGC CGTCCAGTAG CGGGGAGGAA GCCGTTCAGG ACACCATCCT TCGCAAAGAT
    AACCCTTCCC CGACCCACCC GCGAGGCAAA GATTCAGAGT CAGGAGACAA CGTCGCGCGT
    CGACGGCCCG ACCCTGAGAA AGATGATTTT GCTGTGAGGA GAGCGAGGAT GAACCAGACC
    AAGCCAAGGG TGCCGTTGAA TCAACTCCTC TACGGGCCGT ACCAGAAGAG AGAGGCGGGA
    AAATCAGACG GCAGAGAAGA GCACTCGAAG GGAATTTCAG AAAAGAACAG GCTAGAAGAT
    AAAAGATACG GGCCGAGGAC TCCTGTGTCT GATGACGCAG AGAGCTCCAG CCTGCTGGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCACACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTCATAA ATAGCCAGCT GCGCGAGGAG GACGACAAGT GGCAGGACGA CCTGGCTCGT
    TGGAAGAGCC GTAGAAGAAG TGCTTCCCAG GACTTAATCA AGAAAGAGGA AGAGAGGAAG
    AAAATGGAGA AGTTACTGGC TGGAGAGGAT GGGACGAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATCGTTCA AGAAAAGGAG CGGAGAGAGA GGGAGCTGCA TGAGGCCTAT
    AAGAACGCGC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AGCAGTACAT CGAGAGGTTC
    ACCATCAGCG AGGCTGTCCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACGGAGC CAAACTTAAC CCCCTTCCTG AACGACCCTA ACCCCATGAA GTACCTGCGG
    CGACAGTCAC TGCCTCCACC CAAATTCACT GCCACCGTGG AGACCACCAT CGCCCGGACC
    AGCGTTCTGG ATACCGGCGT GTCAGCAGGC GGCGGGTCTC CAAGCAAGAC TGTCCCTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGACGTT
    CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGCATGAATG GAGAAGCAGT TCACGGTGAC
    AAGGAGAAGG AGAGAGAATG TCCCACAGTG GCACCTGCTC CCTCATTAAC CAAACCCCAG
    ATGTTTGAAG CTGTGGCCAG AGTGCACGGG TCCCCAGTGG AGCTCAAACA GGACAACAAT
    AGCATTGAGA TCAACATAAA GAAGCCCAGT TCCGTTCCCC AAGAACTGAG AGCAACCACT
    GAGGAAACTG AACCCAACAG TCAAGAGGAT GAAAATGATG GTGGAAAAAC ACAGAAAGGA
    AATACAGAAC TCGCCCCATC AGAGCCACAG CATTTCACGA CAACTGTGAC TCGGTCCAGC
    CCGACTGTGC CCTTTGTAGA GTTTCCCTCC AGCCCCCAGC GGAGGAACGA TGTGCCACCA
    GAAGAAGACC AGAAGAAACT AGGAAATGAA ATGAGTGGGA AGGTGGAGTT GGTGCTGTCC
    CAAAAGGTGG TAAAGCCAAA ATCACCAGAA CCTGAAGCAA CCCTGACATA TCCATTTCTG
    GAAAAAATGT CTGAAACCAA CCAATTACAC TTGCCAAATC TCAATTCTCA AGGGTCGCCT
    GGCGCTGCCG GCTTCCCGCT CAGAGTTCAG AACTCTTGGC GGCGCTCCCA GTTTTTCTCG
    CAGTCAGTGG ATTCTCCAGG CAGTGAAAAG TCTCCTGTAA CTACTCCTTT CAAGTTTTGG
    GCGTGGGACC CAGAAGAGGA GCGCCGGCGA CAGGAAAAGT GGCAACAGGA GCAGGAACGT
    TTGCTCCAGG AGAGATACCA GAAGGAACAG AACAAGCTGA AGGAAGAGTG GGAAAAGGCC
    CAGAAGGAGG TAGAGGAAGA GGAACGCAGA TACTACGAGG AGGAGCGCAA AATAATTGAA
    GACACTGTGG TTCCATTTAC TCTTTCTTCA AGTTCTGCAG ACCAGCTGTC TACGTCCTCC
    TCTGTGACCG AGGGCACCGG GACAGTGAGT AAGATGGACT TGGAAAATTG TCGGGAGGAA
    GAACAGGAGA CAAAACAGAA GAAGCCACTC CGGGGGGACG ACAGTGATTT AATGCCTAAG
    ACTAGGGAAG ATCACCCCCT GGAGGGAAAG GGCAGCCTAA CTCAAGGGGC CTTGGCTCCT
    TCTAAGAATC CTGGATCGAA AGGAGTCCAT CAGGACCATC AGCTGGATGC AGAAACGGGG
    GCCCTCCGCT GCGGGATGAA CTCCCAGCTA GCTCAGGATC CACCCCAGAA TACGCAGGTA
    TCAAACCCAC CAATGCACGT TTTAGAAGAT GTGAAGCCAA AAACGCTCCC TTTGGATAAA
    AGCATTAACC ATCAGATTGA GTCACCGAGT GAGAGACGGA AGAAAAGCCC TCGAGAGAAT
    TTCCAGGCCG GGCACTTGTC CCCCTGCTCT CCCACCCCTC CTGGCCAATC ACCAAACAGG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AGTGCCGGCC AGCCCACCAC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974249.1
    CDS25..3921
    Translation

    Target ORF information:

    RefSeq Version XM_014118774.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTC CCGCCCATGT GCTGGAAGCC CTTCAGCCTC TGCAGCCGGA GCCGCCCTTC 
    GAGCCCGCTT TCTGCGAGGC GCAGAAGTGG ATCGAGCAAG TAACCGGCAG AAGTTTTGGT
    GACAAAGATT TTCGGACAGG GTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC GGGATTGGAC
    AATATCATCT TATTCTTGAG AGGTTGTAAA GAGCTTGGCC TGAGGGAATC TCAACTTTTT
    GACCCTAGTG ACCTACAGGA TACATCCAAC AGAGTGACCG TCAAGAGCCT CGATTACAGT
    AGGAAACTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAATAGCTCT
    GCGTCCTACA GCGGAACGAC ACTGAACCTG AAGGAGTTCG AAGGCTTGCT GGCTCAGATG
    AGAAAGGAGA CCGATGACAT CGAGAGCCCT AAGCGCAGTA TCCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCCGAGCG CAGCGACTCA CTGTCCCCAC CTCGCCACGG CCGAGATGAT
    TCCTTCGACA GCCTGGACTC CTTCGGGTCC CGATCGCGGC AGACGCCCTC GCCAGATGTT
    GTCCTCCGGG GAAGCAGCGA CGGGAGGGGA AGCGACTCTG AGTCTGACCT GCCGCACCGC
    AAGCTGCCGG ACGTGAAGAA GGATGACATG TCTGCGAGGC GGACGTCCCA CGGCGAGCCC
    AAGTCGGCGG TGCCGTTCAA CCAGTACCTC CCGAACAAAA GCAACCAGAC GGCCTACGTG
    CCGGCGCCTC TGAGGAGGAG GAAGGCTGAG CGGGAGGAGT ACCGGAAGAG CTGGAGCACC
    GCCACCTCGC CCCTGGGCGG GGACCGGCCC TGCAGCTATC CTGAAACGAT AGAGGAAGAG
    GGAAGTGAAG TGGGGTCTCC TTGTGACGAG GACCCCGCCG GCCAAGTGCA GCCCGGTGGG
    AAGCCTTGTG GCAGGGGCTC AGGACGGCCC GCCGGCCGCG GGATGGAGCA GCCTCCTCCG
    GGGGGGCCCA CGGCGGCACC AGCTCCCCAG TCCGAAGACA GAGATGCCGT CGAAGTCCAG
    AAGCGCCGAC GGCTGGAGCA AGCTGGGATC AGAGTGAGGC CGGCGGCGCG GCGCTTTGCC
    AGTCACAAGC CGTCCAGTAG CGGGGAGGAA GCCGTTCAGG ACACCATCCT TCGCAAAGAT
    AACCCTTCCC CGACCCACCC GCGAGGCAAA GATTCAGAGT CAGGAGACAA CGTCGCGCGT
    CGACGGCCCG ACCCTGAGAA AGATGATTTT GCTGTGAGGA GAGCGAGGAT GAACCAGACC
    AAGCCAAGGG TGCCGTTGAA TCAACTCCTC TACGGGCCGT ACCAGAAGAG AGAGGCGGGA
    AAATCAGACG GCAGAGAAGA GCACTCGAAG GGAATTTCAG AAAAGAACAG GCTAGAAGAT
    AAAAGATACG GGCCGAGGAC TCCTGTGTCT GATGACGCAG AGAGCTCCAG CCTGCTGGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCACACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTCATAA ATAGCCAGCT GCGCGAGGAG GACGACAAGT GGCAGGACGA CCTGGCTCGT
    TGGAAGAGCC GTAGAAGAAG TGCTTCCCAG GACTTAATCA AGAAAGAGGA AGAGAGGAAG
    AAAATGGAGA AGTTACTGGC TGGAGAGGAT GGGACGAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATCGTTCA AGAAAAGGAG CGGAGAGAGA GGGAGCTGCA TGAGGCCTAT
    AAGAACGCGC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AGCAGTACAT CGAGAGGTTC
    ACCATCAGCG AGGCTGTCCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACGGAGC CAAACTTAAC CCCCTTCCTG AACGACCCTA ACCCCATGAA GTACCTGCGG
    CGACAGTCAC TGCCTCCACC CAAATTCACT GCCACCGTGG AGACCACCAT CGCCCGGACC
    AGCGTTCTGG ATACCGGCGT GTCAGCAGGC GGCGGGTCTC CAAGCAAGAC TGTCCCTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAACTC TCAAGACGTT
    CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGCATGAATG GAGAAGCAGT TCACGGTGAC
    AAGGAGAAGG AGAGAGAATG TCCCACAGTG GCACCTGCTC CCTCATTAAC CAAACCCCAG
    ATGTTTGAAG CTGTGGCCAG AGTGCACGGG TCCCCAGTGG AGCTCAAACA GGACAACAAT
    AGCATTGAGA TCAACATAAA GAAGCCCAGT TCCGTTCCCC AAGAACTGAG AGCAACCACT
    GAGGAAACTG AACCCAACAG TCAAGAGGAT GAAAATGATG GTGGAAAAAC ACAGAAAGGA
    AATACAGAAC TCGCCCCATC AGAGCCACAG CATTTCACGA CAACTGTGAC TCGGTCCAGC
    CCGACTGTGC CCTTTGTAGA GTTTCCCTCC AGCCCCCAGC GGAGGAACGA TGTGCCACCA
    GAAGAAGACC AGAAGAAACT AGGAAATGAA ATGAGTGGGA AGGTGGAGTT GGTGCTGTCC
    CAAAAGGTGG TAAAGCCAAA ATCACCAGAA CCTGAAGCAA CCCTGACATA TCCATTTCTG
    GAAAAAATGT CTGAAACCAA CCAATTACAC TTGCCAAATC TCAATTCTCA AGGGTCGCCT
    GGCGCTGCCG GCTTCCCGCT CAGAGTTCAG AACTCTTGGC GGCGCTCCCA GTTTTTCTCG
    CAGTCAGTGG ATTCTCCAGG CAGTGAAAAG TCTCCTGTAA CTACTCCTTT CAAGTTTTGG
    GCGTGGGACC CAGAAGAGGA GCGCCGGCGA CAGGAAAAGT GGCAACAGGA GCAGGAACGT
    TTGCTCCAGG AGAGATACCA GAAGGAACAG AACAAGCTGA AGGAAGAGTG GGAAAAGGCC
    CAGAAGGAGG TAGAGGAAGA GGAACGCAGA TACTACGAGG AGGAGCGCAA AATAATTGAA
    GACACTGTGG TTCCATTTAC TCTTTCTTCA AGTTCTGCAG ACCAGCTGTC TACGTCCTCC
    TCTGTGACCG AGGGCACCGG GACAGTGAGT AAGATGGACT TGGAAAATTG TCGGGAGGAA
    GAACAGGAGA CAAAACAGAA GAAGCCACTC CGGGGGGACG ACAGTGATTT AATGCCTAAG
    ACTAGGGAAG ATCACCCCCT GGAGGGAAAG GGCAGCCTAA CTCAAGGGGC CTTGGCTCCT
    TCTAAGAATC CTGGATCGAA AGGAGTCCAT CAGGACCATC AGCTGGATGC AGAAACGGGG
    GCCCTCCGCT GCGGGATGAA CTCCCAGCTA GCTCAGGATC CACCCCAGAA TACGCAGGTA
    TCAAACCCAC CAATGCACGT TTTAGAAGAT GTGAAGCCAA AAACGCTCCC TTTGGATAAA
    AGCATTAACC ATCAGATTGA GTCACCGAGT GAGAGACGGA AGAAAAGCCC TCGAGAGAAT
    TTCCAGGCCG GGCACTTGTC CCCCTGCTCT CCCACCCCTC CTGGCCAATC ACCAAACAGG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AGTGCCGGCC AGCCCACCAC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39786
    Clone ID Related Accession (Same CDS sequence) XM_014118775.1
    Accession Version XM_014118775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGTGGAT
    TCTCCAGGCA GTGAAAAGTC TCCTGTAACT ACTCCTTTCA AGTTTTGGGC GTGGGACCCA
    GAAGAGGAGC GCCGGCGACA GGAAAAGTGG CAACAGGAGC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAACAGAA CAAGCTGAAG GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA
    GAGGAAGAGG AACGCAGATA CTACGAGGAG GAGCGCAAAA TAATTGAAGA CACTGTGGTT
    CCATTTACTC TTTCTTCAAG TTCTGCAGAC CAGCTGTCTA CGTCCTCCTC TGTGACCGAG
    GGCACCGGGA CAGTGAGTAA GATGGACTTG GAAAATTGTC GGGAGGAAGA ACAGGAGACA
    AAACAGAAGA AGCCACTCCG GGGGGACGAC AGTGATTTAA TGCCTAAGAC TAGGGAAGAT
    CACCCCCTGG AGGGAAAGGG CAGCCTAACT CAAGGGGCCT TGGCTCCTTC TAAGAATCCT
    GGATCGAAAG GAGTCCATCA GGACCATCAG CTGGATGCAG AAACGGGGGC CCTCCGCTGC
    GGGATGAACT CCCAGCTAGC TCAGGATCCA CCCCAGAATA CGCAGGTATC AAACCCACCA
    ATGCACGTTT TAGAAGATGT GAAGCCAAAA ACGCTCCCTT TGGATAAAAG CATTAACCAT
    CAGATTGAGT CACCGAGTGA GAGACGGAAG AAAAGCCCTC GAGAGAATTT CCAGGCCGGG
    CACTTGTCCC CCTGCTCTCC CACCCCTCCT GGCCAATCAC CAAACAGGTC GATCAGCGGG
    AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC CTGGGGAAGG GAGCGGCCAT GATCATCGAG
    ACGCTGCAGC TCTACTTCCA CATCCAGTGC TTTCGGTGTG GACTCTGCAA AGGACAACTT
    GGGGACGCGG TGAGCGGGAC AGACGTCAGG ATCCGCAGTG GCCTGCTCAA CTGTAACGAC
    TGCTACCTGC GATCCAGAAG TGCCGGCCAG CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974250.1
    CDS16..3780
    Translation

    Target ORF information:

    RefSeq Version XM_014118775.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGCT ATCCTGAAAC GATAGAGGAA
    GAGGGAAGTG AAGTGGGGTC TCCTTGTGAC GAGGACCCCG CCGGCCAAGT GCAGCCCGGT
    GGGAAGCCTT GTGGCAGGGG CTCAGGACGG CCCGCCGGCC GCGGGATGGA GCAGCCTCCT
    CCGGGGGGGC CCACGGCGGC ACCAGCTCCC CAGTCCGAAG ACAGAGATGC CGTCGAAGTC
    CAGAAGCGCC GACGGCTGGA GCAAGCTGGG ATCAGAGTGA GGCCGGCGGC GCGGCGCTTT
    GCCAGTCACA AGCCGTCCAG TAGCGGGGAG GAAGCCGTTC AGGACACCAT CCTTCGCAAA
    GATAACCCTT CCCCGACCCA CCCGCGAGGC AAAGATTCAG AGTCAGGAGA CAACGTCGCG
    CGTCGACGGC CCGACCCTGA GAAAGATGAT TTTGCTGTGA GGAGAGCGAG GATGAACCAG
    ACCAAGCCAA GGGTGCCGTT GAATCAACTC CTCTACGGGC CGTACCAGAA GAGAGAGGCG
    GGAAAATCAG ACGGCAGAGA AGAGCACTCG AAGGGAATTT CAGAAAAGAA CAGGCTAGAA
    GATAAAAGAT ACGGGCCGAG GACTCCTGTG TCTGATGACG CAGAGAGCTC CAGCCTGCTG
    GACATGCGGT GTGAGGAGGA GGCCGCGGTG CAGCCACACA GCAGGGCCCG CCAGGAGCAG
    CTGCAGCTCA TAAATAGCCA GCTGCGCGAG GAGGACGACA AGTGGCAGGA CGACCTGGCT
    CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC CAGGACTTAA TCAAGAAAGA GGAAGAGAGG
    AAGAAAATGG AGAAGTTACT GGCTGGAGAG GATGGGACGA GTGAACGAAG GAAAAGCATC
    AAAACCTACA GAGAAATCGT TCAAGAAAAG GAGCGGAGAG AGAGGGAGCT GCATGAGGCC
    TATAAGAACG CGCGGTCCCA GGAGGAGGCA GAGGGGATCC TTCAGCAGTA CATCGAGAGG
    TTCACCATCA GCGAGGCTGT CCTCGAACGC TTGGAGATGC CAAAAATTCT GGAAAGAAGC
    CATTCAACGG AGCCAAACTT AACCCCCTTC CTGAACGACC CTAACCCCAT GAAGTACCTG
    CGGCGACAGT CACTGCCTCC ACCCAAATTC ACTGCCACCG TGGAGACCAC CATCGCCCGG
    ACCAGCGTTC TGGATACCGG CGTGTCAGCA GGCGGCGGGT CTCCAAGCAA GACTGTCCCT
    CCCAAAGCAG TGCCTATGCT GACACCCAAG CCTTACTCCC AGCCCAAAAA CTCTCAAGAC
    GTTCTGAAGA CCTTTAAGGT AGATGGGAAA GTCAGCATGA ATGGAGAAGC AGTTCACGGT
    GACAAGGAGA AGGAGAGAGA ATGTCCCACA GTGGCACCTG CTCCCTCATT AACCAAACCC
    CAGATGTTTG AAGCTGTGGC CAGAGTGCAC GGGTCCCCAG TGGAGCTCAA ACAGGACAAC
    AATAGCATTG AGATCAACAT AAAGAAGCCC AGTTCCGTTC CCCAAGAACT GAGAGCAACC
    ACTGAGGAAA CTGAACCCAA CAGTCAAGAG GATGAAAATG ATGGTGGAAA AACACAGAAA
    GGAAATACAG AACTCGCCCC ATCAGAGCCA CAGCATTTCA CGACAACTGT GACTCGGTCC
    AGCCCGACTG TGCCCTTTGT AGAGTTTCCC TCCAGCCCCC AGCGGAGGAA CGATGTGCCA
    CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT GAAATGAGTG GGAAGGTGGA GTTGGTGCTG
    TCCCAAAAGG TGGTAAAGCC AAAATCACCA GAACCTGAAG CAACCCTGAC ATATCCATTT
    CTGGAAAAAA TGTCTGAAAC CAACCAATTA CACTTGCCAA ATCTCAATTC TCAAGTGGAT
    TCTCCAGGCA GTGAAAAGTC TCCTGTAACT ACTCCTTTCA AGTTTTGGGC GTGGGACCCA
    GAAGAGGAGC GCCGGCGACA GGAAAAGTGG CAACAGGAGC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAACAGAA CAAGCTGAAG GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA
    GAGGAAGAGG AACGCAGATA CTACGAGGAG GAGCGCAAAA TAATTGAAGA CACTGTGGTT
    CCATTTACTC TTTCTTCAAG TTCTGCAGAC CAGCTGTCTA CGTCCTCCTC TGTGACCGAG
    GGCACCGGGA CAGTGAGTAA GATGGACTTG GAAAATTGTC GGGAGGAAGA ACAGGAGACA
    AAACAGAAGA AGCCACTCCG GGGGGACGAC AGTGATTTAA TGCCTAAGAC TAGGGAAGAT
    CACCCCCTGG AGGGAAAGGG CAGCCTAACT CAAGGGGCCT TGGCTCCTTC TAAGAATCCT
    GGATCGAAAG GAGTCCATCA GGACCATCAG CTGGATGCAG AAACGGGGGC CCTCCGCTGC
    GGGATGAACT CCCAGCTAGC TCAGGATCCA CCCCAGAATA CGCAGGTATC AAACCCACCA
    ATGCACGTTT TAGAAGATGT GAAGCCAAAA ACGCTCCCTT TGGATAAAAG CATTAACCAT
    CAGATTGAGT CACCGAGTGA GAGACGGAAG AAAAGCCCTC GAGAGAATTT CCAGGCCGGG
    CACTTGTCCC CCTGCTCTCC CACCCCTCCT GGCCAATCAC CAAACAGGTC GATCAGCGGG
    AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC CTGGGGAAGG GAGCGGCCAT GATCATCGAG
    ACGCTGCAGC TCTACTTCCA CATCCAGTGC TTTCGGTGTG GACTCTGCAA AGGACAACTT
    GGGGACGCGG TGAGCGGGAC AGACGTCAGG ATCCGCAGTG GCCTGCTCAA CTGTAACGAC
    TGCTACCTGC GATCCAGAAG TGCCGGCCAG CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39787
    Clone ID Related Accession (Same CDS sequence) XM_014118776.1
    Accession Version XM_014118776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTTCTG CAGTAGCACG AACAGTCATC ACCATAAATA CTCGGGCCTC GGAACGCTAC 
    CAGCGGAGGG CTGTAAACAC ATCAATCACC AGCCGTGCAG AAATTCCGTT CTCAGCTGTC
    ATCTCAGTTT CAGCCGCAAA GATTAAACGC CTGCATTTGA ATTCAAAGCA CCGCACGGAT
    AGGATCCACG GAGACAATAT CATCTTATTC TTGAGAGGTT GTAAAGAGCT TGGCCTGAGG
    GAATCTCAAC TTTTTGACCC TAGTGACCTA CAGGATACAT CCAACAGAGT GACCGTCAAG
    AGCCTCGATT ACAGTAGGAA ACTGAAAAAT GTATTAGTTA CCATTTACTG GCTGGGAAAA
    GCAGCAAATA GCTCTGCGTC CTACAGCGGA ACGACACTGA ACCTGAAGGA GTTCGAAGGC
    TTGCTGGCTC AGATGAGAAA GGAGACCGAT GACATCGAGA GCCCTAAGCG CAGTATCCGG
    GACAGCGGCT ACATAGACTG CTGGGACTCC GAGCGCAGCG ACTCACTGTC CCCACCTCGC
    CACGGCCGAG ATGATTCCTT CGACAGCCTG GACTCCTTCG GGTCCCGATC GCGGCAGACG
    CCCTCGCCAG ATGTTGTCCT CCGGGGAAGC AGCGACGGGA GGGGAAGCGA CTCTGAGTCT
    GACCTGCCGC ACCGCAAGCT GCCGGACGTG AAGAAGGATG ACATGTCTGC GAGGCGGACG
    TCCCACGGCG AGCCCAAGTC GGCGGTGCCG TTCAACCAGT ACCTCCCGAA CAAAAGCAAC
    CAGACGGCCT ACGTGCCGGC GCCTCTGAGG AGGAGGAAGG CTGAGCGGGA GGAGTACCGG
    AAGAGCTGGA GCACCGCCAC CTCGCCCCTG GGCGGGGACC GGCCCTGCAG CTATCCTGAA
    ACGATAGAGG AAGAGGGAAG TGAAGTGGGG TCTCCTTGTG ACGAGGACCC CGCCGGCCAA
    GTGCAGCCCG GTGGGAAGCC TTGTGGCAGG GGCTCAGGAC GGCCCGCCGG CCGCGGGATG
    GAGCAGCCTC CTCCGGGGGG GCCCACGGCG GCACCAGCTC CCCAGTCCGA AGACAGAGAT
    GCCGTCGAAG TCCAGAAGCG CCGACGGCTG GAGCAAGCTG GGATCAGAGT GAGGCCGGCG
    GCGCGGCGCT TTGCCAGTCA CAAGCCGTCC AGTAGCGGGG AGGAAGCCGT TCAGGACACC
    ATCCTTCGCA AAGATAACCC TTCCCCGACC CACCCGCGAG GCAAAGATTC AGAGTCAGGA
    GACAACGTCG CGCGTCGACG GCCCGACCCT GAGAAAGATG ATTTTGCTGT GAGGAGAGCG
    AGGATGAACC AGACCAAGCC AAGGGTGCCG TTGAATCAAC TCCTCTACGG GCCGTACCAG
    AAGAGAGAGG CGGGAAAATC AGACGGCAGA GAAGAGCACT CGAAGGGAAT TTCAGAAAAG
    AACAGGCTAG AAGATAAAAG ATACGGGCCG AGGACTCCTG TGTCTGATGA CGCAGAGAGC
    TCCAGCCTGC TGGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCACA CAGCAGGGCC
    CGCCAGGAGC AGCTGCAGCT CATAAATAGC CAGCTGCGCG AGGAGGACGA CAAGTGGCAG
    GACGACCTGG CTCGTTGGAA GAGCCGTAGA AGAAGTGCTT CCCAGGACTT AATCAAGAAA
    GAGGAAGAGA GGAAGAAAAT GGAGAAGTTA CTGGCTGGAG AGGATGGGAC GAGTGAACGA
    AGGAAAAGCA TCAAAACCTA CAGAGAAATC GTTCAAGAAA AGGAGCGGAG AGAGAGGGAG
    CTGCATGAGG CCTATAAGAA CGCGCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAGCAG
    TACATCGAGA GGTTCACCAT CAGCGAGGCT GTCCTCGAAC GCTTGGAGAT GCCAAAAATT
    CTGGAAAGAA GCCATTCAAC GGAGCCAAAC TTAACCCCCT TCCTGAACGA CCCTAACCCC
    ATGAAGTACC TGCGGCGACA GTCACTGCCT CCACCCAAAT TCACTGCCAC CGTGGAGACC
    ACCATCGCCC GGACCAGCGT TCTGGATACC GGCGTGTCAG CAGGCGGCGG GTCTCCAAGC
    AAGACTGTCC CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA
    AACTCTCAAG ACGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGCAT GAATGGAGAA
    GCAGTTCACG GTGACAAGGA GAAGGAGAGA GAATGTCCCA CAGTGGCACC TGCTCCCTCA
    TTAACCAAAC CCCAGATGTT TGAAGCTGTG GCCAGAGTGC ACGGGTCCCC AGTGGAGCTC
    AAACAGGACA ACAATAGCAT TGAGATCAAC ATAAAGAAGC CCAGTTCCGT TCCCCAAGAA
    CTGAGAGCAA CCACTGAGGA AACTGAACCC AACAGTCAAG AGGATGAAAA TGATGGTGGA
    AAAACACAGA AAGGAAATAC AGAACTCGCC CCATCAGAGC CACAGCATTT CACGACAACT
    GTGACTCGGT CCAGCCCGAC TGTGCCCTTT GTAGAGTTTC CCTCCAGCCC CCAGCGGAGG
    AACGATGTGC CACCAGAAGA AGACCAGAAG AAACTAGGAA ATGAAATGAG TGGGAAGGTG
    GAGTTGGTGC TGTCCCAAAA GGTGGTAAAG CCAAAATCAC CAGAACCTGA AGCAACCCTG
    ACATATCCAT TTCTGGAAAA AATGTCTGAA ACCAACCAAT TACACTTGCC AAATCTCAAT
    TCTCAAGGGT CGCCTGGCGC TGCCGGCTTC CCGCTCAGAG TTCAGAACTC TTGGCGGCGC
    TCCCAGTTTT TCTCGCAGTC AGTGGATTCT CCAGGCAGTG AAAAGTCTCC TGTAACTACT
    CCTTTCAAGT TTTGGGCGTG GGACCCAGAA GAGGAGCGCC GGCGACAGGA AAAGTGGCAA
    CAGGAGCAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AACAGAACAA GCTGAAGGAA
    GAGTGGGAAA AGGCCCAGAA GGAGGTAGAG GAAGAGGAAC GCAGATACTA CGAGGAGGAG
    CGCAAAATAA TTGAAGACAC TGTGGTTCCA TTTACTCTTT CTTCAAGTTC TGCAGACCAG
    CTGTCTACGT CCTCCTCTGT GACCGAGGGC ACCGGGACAG TGAGTAAGAT GGACTTGGAA
    AATTGTCGGG AGGAAGAACA GGAGACAAAA CAGAAGAAGC CACTCCGGGG GGACGACAGT
    GATTTAATGC CTAAGACTAG GGAAGATCAC CCCCTGGAGG GAAAGGGCAG CCTAACTCAA
    GGGGCCTTGG CTCCTTCTAA GAATCCTGGA TCGAAAGGAG TCCATCAGGA CCATCAGCTG
    GATGCAGAAA CGGGGGCCCT CCGCTGCGGG ATGAACTCCC AGCTAGCTCA GGATCCACCC
    CAGAATACGC AGGTATCAAA CCCACCAATG CACGTTTTAG AAGATGTGAA GCCAAAAACG
    CTCCCTTTGG ATAAAAGCAT TAACCATCAG ATTGAGTCAC CGAGTGAGAG ACGGAAGAAA
    AGCCCTCGAG AGAATTTCCA GGCCGGGCAC TTGTCCCCCT GCTCTCCCAC CCCTCCTGGC
    CAATCACCAA ACAGGTCGAT CAGCGGGAAG AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG
    GGGAAGGGAG CGGCCATGAT CATCGAGACG CTGCAGCTCT ACTTCCACAT CCAGTGCTTT
    CGGTGTGGAC TCTGCAAAGG ACAACTTGGG GACGCGGTGA GCGGGACAGA CGTCAGGATC
    CGCAGTGGCC TGCTCAACTG TAACGACTGC TACCTGCGAT CCAGAAGTGC CGGCCAGCCC
    ACCACCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974251.1
    CDS16..3867
    Translation

    Target ORF information:

    RefSeq Version XM_014118776.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTTCTG CAGTAGCACG AACAGTCATC ACCATAAATA CTCGGGCCTC GGAACGCTAC 
    CAGCGGAGGG CTGTAAACAC ATCAATCACC AGCCGTGCAG AAATTCCGTT CTCAGCTGTC
    ATCTCAGTTT CAGCCGCAAA GATTAAACGC CTGCATTTGA ATTCAAAGCA CCGCACGGAT
    AGGATCCACG GAGACAATAT CATCTTATTC TTGAGAGGTT GTAAAGAGCT TGGCCTGAGG
    GAATCTCAAC TTTTTGACCC TAGTGACCTA CAGGATACAT CCAACAGAGT GACCGTCAAG
    AGCCTCGATT ACAGTAGGAA ACTGAAAAAT GTATTAGTTA CCATTTACTG GCTGGGAAAA
    GCAGCAAATA GCTCTGCGTC CTACAGCGGA ACGACACTGA ACCTGAAGGA GTTCGAAGGC
    TTGCTGGCTC AGATGAGAAA GGAGACCGAT GACATCGAGA GCCCTAAGCG CAGTATCCGG
    GACAGCGGCT ACATAGACTG CTGGGACTCC GAGCGCAGCG ACTCACTGTC CCCACCTCGC
    CACGGCCGAG ATGATTCCTT CGACAGCCTG GACTCCTTCG GGTCCCGATC GCGGCAGACG
    CCCTCGCCAG ATGTTGTCCT CCGGGGAAGC AGCGACGGGA GGGGAAGCGA CTCTGAGTCT
    GACCTGCCGC ACCGCAAGCT GCCGGACGTG AAGAAGGATG ACATGTCTGC GAGGCGGACG
    TCCCACGGCG AGCCCAAGTC GGCGGTGCCG TTCAACCAGT ACCTCCCGAA CAAAAGCAAC
    CAGACGGCCT ACGTGCCGGC GCCTCTGAGG AGGAGGAAGG CTGAGCGGGA GGAGTACCGG
    AAGAGCTGGA GCACCGCCAC CTCGCCCCTG GGCGGGGACC GGCCCTGCAG CTATCCTGAA
    ACGATAGAGG AAGAGGGAAG TGAAGTGGGG TCTCCTTGTG ACGAGGACCC CGCCGGCCAA
    GTGCAGCCCG GTGGGAAGCC TTGTGGCAGG GGCTCAGGAC GGCCCGCCGG CCGCGGGATG
    GAGCAGCCTC CTCCGGGGGG GCCCACGGCG GCACCAGCTC CCCAGTCCGA AGACAGAGAT
    GCCGTCGAAG TCCAGAAGCG CCGACGGCTG GAGCAAGCTG GGATCAGAGT GAGGCCGGCG
    GCGCGGCGCT TTGCCAGTCA CAAGCCGTCC AGTAGCGGGG AGGAAGCCGT TCAGGACACC
    ATCCTTCGCA AAGATAACCC TTCCCCGACC CACCCGCGAG GCAAAGATTC AGAGTCAGGA
    GACAACGTCG CGCGTCGACG GCCCGACCCT GAGAAAGATG ATTTTGCTGT GAGGAGAGCG
    AGGATGAACC AGACCAAGCC AAGGGTGCCG TTGAATCAAC TCCTCTACGG GCCGTACCAG
    AAGAGAGAGG CGGGAAAATC AGACGGCAGA GAAGAGCACT CGAAGGGAAT TTCAGAAAAG
    AACAGGCTAG AAGATAAAAG ATACGGGCCG AGGACTCCTG TGTCTGATGA CGCAGAGAGC
    TCCAGCCTGC TGGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCACA CAGCAGGGCC
    CGCCAGGAGC AGCTGCAGCT CATAAATAGC CAGCTGCGCG AGGAGGACGA CAAGTGGCAG
    GACGACCTGG CTCGTTGGAA GAGCCGTAGA AGAAGTGCTT CCCAGGACTT AATCAAGAAA
    GAGGAAGAGA GGAAGAAAAT GGAGAAGTTA CTGGCTGGAG AGGATGGGAC GAGTGAACGA
    AGGAAAAGCA TCAAAACCTA CAGAGAAATC GTTCAAGAAA AGGAGCGGAG AGAGAGGGAG
    CTGCATGAGG CCTATAAGAA CGCGCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAGCAG
    TACATCGAGA GGTTCACCAT CAGCGAGGCT GTCCTCGAAC GCTTGGAGAT GCCAAAAATT
    CTGGAAAGAA GCCATTCAAC GGAGCCAAAC TTAACCCCCT TCCTGAACGA CCCTAACCCC
    ATGAAGTACC TGCGGCGACA GTCACTGCCT CCACCCAAAT TCACTGCCAC CGTGGAGACC
    ACCATCGCCC GGACCAGCGT TCTGGATACC GGCGTGTCAG CAGGCGGCGG GTCTCCAAGC
    AAGACTGTCC CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA
    AACTCTCAAG ACGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGCAT GAATGGAGAA
    GCAGTTCACG GTGACAAGGA GAAGGAGAGA GAATGTCCCA CAGTGGCACC TGCTCCCTCA
    TTAACCAAAC CCCAGATGTT TGAAGCTGTG GCCAGAGTGC ACGGGTCCCC AGTGGAGCTC
    AAACAGGACA ACAATAGCAT TGAGATCAAC ATAAAGAAGC CCAGTTCCGT TCCCCAAGAA
    CTGAGAGCAA CCACTGAGGA AACTGAACCC AACAGTCAAG AGGATGAAAA TGATGGTGGA
    AAAACACAGA AAGGAAATAC AGAACTCGCC CCATCAGAGC CACAGCATTT CACGACAACT
    GTGACTCGGT CCAGCCCGAC TGTGCCCTTT GTAGAGTTTC CCTCCAGCCC CCAGCGGAGG
    AACGATGTGC CACCAGAAGA AGACCAGAAG AAACTAGGAA ATGAAATGAG TGGGAAGGTG
    GAGTTGGTGC TGTCCCAAAA GGTGGTAAAG CCAAAATCAC CAGAACCTGA AGCAACCCTG
    ACATATCCAT TTCTGGAAAA AATGTCTGAA ACCAACCAAT TACACTTGCC AAATCTCAAT
    TCTCAAGGGT CGCCTGGCGC TGCCGGCTTC CCGCTCAGAG TTCAGAACTC TTGGCGGCGC
    TCCCAGTTTT TCTCGCAGTC AGTGGATTCT CCAGGCAGTG AAAAGTCTCC TGTAACTACT
    CCTTTCAAGT TTTGGGCGTG GGACCCAGAA GAGGAGCGCC GGCGACAGGA AAAGTGGCAA
    CAGGAGCAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AACAGAACAA GCTGAAGGAA
    GAGTGGGAAA AGGCCCAGAA GGAGGTAGAG GAAGAGGAAC GCAGATACTA CGAGGAGGAG
    CGCAAAATAA TTGAAGACAC TGTGGTTCCA TTTACTCTTT CTTCAAGTTC TGCAGACCAG
    CTGTCTACGT CCTCCTCTGT GACCGAGGGC ACCGGGACAG TGAGTAAGAT GGACTTGGAA
    AATTGTCGGG AGGAAGAACA GGAGACAAAA CAGAAGAAGC CACTCCGGGG GGACGACAGT
    GATTTAATGC CTAAGACTAG GGAAGATCAC CCCCTGGAGG GAAAGGGCAG CCTAACTCAA
    GGGGCCTTGG CTCCTTCTAA GAATCCTGGA TCGAAAGGAG TCCATCAGGA CCATCAGCTG
    GATGCAGAAA CGGGGGCCCT CCGCTGCGGG ATGAACTCCC AGCTAGCTCA GGATCCACCC
    CAGAATACGC AGGTATCAAA CCCACCAATG CACGTTTTAG AAGATGTGAA GCCAAAAACG
    CTCCCTTTGG ATAAAAGCAT TAACCATCAG ATTGAGTCAC CGAGTGAGAG ACGGAAGAAA
    AGCCCTCGAG AGAATTTCCA GGCCGGGCAC TTGTCCCCCT GCTCTCCCAC CCCTCCTGGC
    CAATCACCAA ACAGGTCGAT CAGCGGGAAG AAGCTGTGCT CGTCCTGTGG GCTCCCCCTG
    GGGAAGGGAG CGGCCATGAT CATCGAGACG CTGCAGCTCT ACTTCCACAT CCAGTGCTTT
    CGGTGTGGAC TCTGCAAAGG ACAACTTGGG GACGCGGTGA GCGGGACAGA CGTCAGGATC
    CGCAGTGGCC TGCTCAACTG TAACGACTGC TACCTGCGAT CCAGAAGTGC CGGCCAGCCC
    ACCACCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39788
    Clone ID Related Accession (Same CDS sequence) XM_014118777.1
    Accession Version XM_014118777.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGTGCAG ATGACTCAGA GACCCAAGAC CTCTTCCAGT TGCAAGTAAC CGGCAGAAGT 
    TTTGGTGACA AAGATTTTCG GACAGGGTTA GAAAATGGAA TCCTCCTCTG CGAGTTGCTG
    AATGCTATAA AGCCAGGACT TGTTAAAAAG ATCAATAGAT TGCCTACCCC CATTGCGGGA
    TTGGACAATA TCATCTTATT CTTGAGAGGT TGTAAAGAGC TTGGCCTGAG GGAATCTCAA
    CTTTTTGACC CTAGTGACCT ACAGGATACA TCCAACAGAG TGACCGTCAA GAGCCTCGAT
    TACAGTAGGA AACTGAAAAA TGTATTAGTT ACCATTTACT GGCTGGGAAA AGCAGCAAAT
    AGCTCTGCGT CCTACAGCGG AACGACACTG AACCTGAAGG AGTTCGAAGG CTTGCTGGCT
    CAGATGAGAA AGGAGACCGA TGACATCGAG AGCCCTAAGC GCAGTATCCG GGACAGCGGC
    TACATAGACT GCTGGGACTC CGAGCGCAGC GACTCACTGT CCCCACCTCG CCACGGCCGA
    GATGATTCCT TCGACAGCCT GGACTCCTTC GGGTCCCGAT CGCGGCAGAC GCCCTCGCCA
    GATGTTGTCC TCCGGGGAAG CAGCGACGGG AGGGGAAGCG ACTCTGAGTC TGACCTGCCG
    CACCGCAAGC TGCCGGACGT GAAGAAGGAT GACATGTCTG CGAGGCGGAC GTCCCACGGC
    GAGCCCAAGT CGGCGGTGCC GTTCAACCAG TACCTCCCGA ACAAAAGCAA CCAGACGGCC
    TACGTGCCGG CGCCTCTGAG GAGGAGGAAG GCTGAGCGGG AGGAGTACCG GAAGAGCTGG
    AGCACCGCCA CCTCGCCCCT GGGCGGGGAC CGGCCCTGCA GCTATCCTGA AACGATAGAG
    GAAGAGGGAA GTGAAGTGGG GTCTCCTTGT GACGAGGACC CCGCCGGCCA AGTGCAGCCC
    GGTGGGAAGC CTTGTGGCAG GGGCTCAGGA CGGCCCGCCG GCCGCGGGAT GGAGCAGCCT
    CCTCCGGGGG GGCCCACGGC GGCACCAGCT CCCCAGTCCG AAGACAGAGA TGCCGTCGAA
    GTCCAGAAGC GCCGACGGCT GGAGCAAGCT GGGATCAGAG TGAGGCCGGC GGCGCGGCGC
    TTTGCCAGTC ACAAGCCGTC CAGTAGCGGG GAGGAAGCCG TTCAGGACAC CATCCTTCGC
    AAAGATAACC CTTCCCCGAC CCACCCGCGA GGCAAAGATT CAGAGTCAGG AGACAACGTC
    GCGCGTCGAC GGCCCGACCC TGAGAAAGAT GATTTTGCTG TGAGGAGAGC GAGGATGAAC
    CAGACCAAGC CAAGGGTGCC GTTGAATCAA CTCCTCTACG GGCCGTACCA GAAGAGAGAG
    GCGGGAAAAT CAGACGGCAG AGAAGAGCAC TCGAAGGGAA TTTCAGAAAA GAACAGGCTA
    GAAGATAAAA GATACGGGCC GAGGACTCCT GTGTCTGATG ACGCAGAGAG CTCCAGCCTG
    CTGGACATGC GGTGTGAGGA GGAGGCCGCG GTGCAGCCAC ACAGCAGGGC CCGCCAGGAG
    CAGCTGCAGC TCATAAATAG CCAGCTGCGC GAGGAGGACG ACAAGTGGCA GGACGACCTG
    GCTCGTTGGA AGAGCCGTAG AAGAAGTGCT TCCCAGGACT TAATCAAGAA AGAGGAAGAG
    AGGAAGAAAA TGGAGAAGTT ACTGGCTGGA GAGGATGGGA CGAGTGAACG AAGGAAAAGC
    ATCAAAACCT ACAGAGAAAT CGTTCAAGAA AAGGAGCGGA GAGAGAGGGA GCTGCATGAG
    GCCTATAAGA ACGCGCGGTC CCAGGAGGAG GCAGAGGGGA TCCTTCAGCA GTACATCGAG
    AGGTTCACCA TCAGCGAGGC TGTCCTCGAA CGCTTGGAGA TGCCAAAAAT TCTGGAAAGA
    AGCCATTCAA CGGAGCCAAA CTTAACCCCC TTCCTGAACG ACCCTAACCC CATGAAGTAC
    CTGCGGCGAC AGTCACTGCC TCCACCCAAA TTCACTGCCA CCGTGGAGAC CACCATCGCC
    CGGACCAGCG TTCTGGATAC CGGCGTGTCA GCAGGCGGCG GGTCTCCAAG CAAGACTGTC
    CCTCCCAAAG CAGTGCCTAT GCTGACACCC AAGCCTTACT CCCAGCCCAA AAACTCTCAA
    GACGTTCTGA AGACCTTTAA GGTAGATGGG AAAGTCAGCA TGAATGGAGA AGCAGTTCAC
    GGTGACAAGG AGAAGGAGAG AGAATGTCCC ACAGTGGCAC CTGCTCCCTC ATTAACCAAA
    CCCCAGATGT TTGAAGCTGT GGCCAGAGTG CACGGGTCCC CAGTGGAGCT CAAACAGGAC
    AACAATAGCA TTGAGATCAA CATAAAGAAG CCCAGTTCCG TTCCCCAAGA ACTGAGAGCA
    ACCACTGAGG AAACTGAACC CAACAGTCAA GAGGATGAAA ATGATGGTGG AAAAACACAG
    AAAGGAAATA CAGAACTCGC CCCATCAGAG CCACAGCATT TCACGACAAC TGTGACTCGG
    TCCAGCCCGA CTGTGCCCTT TGTAGAGTTT CCCTCCAGCC CCCAGCGGAG GAACGATGTG
    CCACCAGAAG AAGACCAGAA GAAACTAGGA AATGAAATGA GTGGGAAGGT GGAGTTGGTG
    CTGTCCCAAA AGGTGGTAAA GCCAAAATCA CCAGAACCTG AAGCAACCCT GACATATCCA
    TTTCTGGAAA AAATGTCTGA AACCAACCAA TTACACTTGC CAAATCTCAA TTCTCAAGGG
    TCGCCTGGCG CTGCCGGCTT CCCGCTCAGA GTTCAGAACT CTTGGCGGCG CTCCCAGTTT
    TTCTCGCAGT CAGTGGATTC TCCAGGCAGT GAAAAGTCTC CTGTAACTAC TCCTTTCAAG
    TTTTGGGCGT GGGACCCAGA AGAGGAGCGC CGGCGACAGG AAAAGTGGCA ACAGGAGCAG
    GAACGTTTGC TCCAGGAGAG ATACCAGAAG GAACAGAACA AGCTGAAGGA AGAGTGGGAA
    AAGGCCCAGA AGGAGGTAGA GGAAGAGGAA CGCAGATACT ACGAGGAGGA GCGCAAAATA
    ATTGAAGACA CTGTGGTTCC ATTTACTCTT TCTTCAAGTT CTGCAGACCA GCTGTCTACG
    TCCTCCTCTG TGACCGAGGG CACCGGGACA GTGAGTAAGA TGGACTTGGA AAATTGTCGG
    GAGGAAGAAC AGGAGACAAA ACAGAAGAAG CCACTCCGGG GGGACGACAG TGATTTAATG
    CCTAAGACTA GGGAAGATCA CCCCCTGGAG GGAAAGGGCA GCCTAACTCA AGGGGCCTTG
    GCTCCTTCTA AGAATCCTGG ATCGAAAGGA GTCCATCAGG ACCATCAGCT GGATGCAGAA
    ACGGGGGCCC TCCGCTGCGG GATGAACTCC CAGCTAGCTC AGGATCCACC CCAGAATACG
    CAGGTATCAA ACCCACCAAT GCACGTTTTA GAAGATGTGA AGCCAAAAAC GCTCCCTTTG
    GATAAAAGCA TTAACCATCA GATTGAGTCA CCGAGTGAGA GACGGAAGAA AAGCCCTCGA
    GAGAATTTCC AGGCCGGGCA CTTGTCCCCC TGCTCTCCCA CCCCTCCTGG CCAATCACCA
    AACAGGTCGA TCAGCGGGAA GAAGCTGTGC TCGTCCTGTG GGCTCCCCCT GGGGAAGGGA
    GCGGCCATGA TCATCGAGAC GCTGCAGCTC TACTTCCACA TCCAGTGCTT TCGGTGTGGA
    CTCTGCAAAG GACAACTTGG GGACGCGGTG AGCGGGACAG ACGTCAGGAT CCGCAGTGGC
    CTGCTCAACT GTAACGACTG CTACCTGCGA TCCAGAAGTG CCGGCCAGCC CACCACCCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974252.1
    CDS16..3858
    Translation

    Target ORF information:

    RefSeq Version XM_014118777.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118777.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGTGCAG ATGACTCAGA GACCCAAGAC CTCTTCCAGT TGCAAGTAAC CGGCAGAAGT 
    TTTGGTGACA AAGATTTTCG GACAGGGTTA GAAAATGGAA TCCTCCTCTG CGAGTTGCTG
    AATGCTATAA AGCCAGGACT TGTTAAAAAG ATCAATAGAT TGCCTACCCC CATTGCGGGA
    TTGGACAATA TCATCTTATT CTTGAGAGGT TGTAAAGAGC TTGGCCTGAG GGAATCTCAA
    CTTTTTGACC CTAGTGACCT ACAGGATACA TCCAACAGAG TGACCGTCAA GAGCCTCGAT
    TACAGTAGGA AACTGAAAAA TGTATTAGTT ACCATTTACT GGCTGGGAAA AGCAGCAAAT
    AGCTCTGCGT CCTACAGCGG AACGACACTG AACCTGAAGG AGTTCGAAGG CTTGCTGGCT
    CAGATGAGAA AGGAGACCGA TGACATCGAG AGCCCTAAGC GCAGTATCCG GGACAGCGGC
    TACATAGACT GCTGGGACTC CGAGCGCAGC GACTCACTGT CCCCACCTCG CCACGGCCGA
    GATGATTCCT TCGACAGCCT GGACTCCTTC GGGTCCCGAT CGCGGCAGAC GCCCTCGCCA
    GATGTTGTCC TCCGGGGAAG CAGCGACGGG AGGGGAAGCG ACTCTGAGTC TGACCTGCCG
    CACCGCAAGC TGCCGGACGT GAAGAAGGAT GACATGTCTG CGAGGCGGAC GTCCCACGGC
    GAGCCCAAGT CGGCGGTGCC GTTCAACCAG TACCTCCCGA ACAAAAGCAA CCAGACGGCC
    TACGTGCCGG CGCCTCTGAG GAGGAGGAAG GCTGAGCGGG AGGAGTACCG GAAGAGCTGG
    AGCACCGCCA CCTCGCCCCT GGGCGGGGAC CGGCCCTGCA GCTATCCTGA AACGATAGAG
    GAAGAGGGAA GTGAAGTGGG GTCTCCTTGT GACGAGGACC CCGCCGGCCA AGTGCAGCCC
    GGTGGGAAGC CTTGTGGCAG GGGCTCAGGA CGGCCCGCCG GCCGCGGGAT GGAGCAGCCT
    CCTCCGGGGG GGCCCACGGC GGCACCAGCT CCCCAGTCCG AAGACAGAGA TGCCGTCGAA
    GTCCAGAAGC GCCGACGGCT GGAGCAAGCT GGGATCAGAG TGAGGCCGGC GGCGCGGCGC
    TTTGCCAGTC ACAAGCCGTC CAGTAGCGGG GAGGAAGCCG TTCAGGACAC CATCCTTCGC
    AAAGATAACC CTTCCCCGAC CCACCCGCGA GGCAAAGATT CAGAGTCAGG AGACAACGTC
    GCGCGTCGAC GGCCCGACCC TGAGAAAGAT GATTTTGCTG TGAGGAGAGC GAGGATGAAC
    CAGACCAAGC CAAGGGTGCC GTTGAATCAA CTCCTCTACG GGCCGTACCA GAAGAGAGAG
    GCGGGAAAAT CAGACGGCAG AGAAGAGCAC TCGAAGGGAA TTTCAGAAAA GAACAGGCTA
    GAAGATAAAA GATACGGGCC GAGGACTCCT GTGTCTGATG ACGCAGAGAG CTCCAGCCTG
    CTGGACATGC GGTGTGAGGA GGAGGCCGCG GTGCAGCCAC ACAGCAGGGC CCGCCAGGAG
    CAGCTGCAGC TCATAAATAG CCAGCTGCGC GAGGAGGACG ACAAGTGGCA GGACGACCTG
    GCTCGTTGGA AGAGCCGTAG AAGAAGTGCT TCCCAGGACT TAATCAAGAA AGAGGAAGAG
    AGGAAGAAAA TGGAGAAGTT ACTGGCTGGA GAGGATGGGA CGAGTGAACG AAGGAAAAGC
    ATCAAAACCT ACAGAGAAAT CGTTCAAGAA AAGGAGCGGA GAGAGAGGGA GCTGCATGAG
    GCCTATAAGA ACGCGCGGTC CCAGGAGGAG GCAGAGGGGA TCCTTCAGCA GTACATCGAG
    AGGTTCACCA TCAGCGAGGC TGTCCTCGAA CGCTTGGAGA TGCCAAAAAT TCTGGAAAGA
    AGCCATTCAA CGGAGCCAAA CTTAACCCCC TTCCTGAACG ACCCTAACCC CATGAAGTAC
    CTGCGGCGAC AGTCACTGCC TCCACCCAAA TTCACTGCCA CCGTGGAGAC CACCATCGCC
    CGGACCAGCG TTCTGGATAC CGGCGTGTCA GCAGGCGGCG GGTCTCCAAG CAAGACTGTC
    CCTCCCAAAG CAGTGCCTAT GCTGACACCC AAGCCTTACT CCCAGCCCAA AAACTCTCAA
    GACGTTCTGA AGACCTTTAA GGTAGATGGG AAAGTCAGCA TGAATGGAGA AGCAGTTCAC
    GGTGACAAGG AGAAGGAGAG AGAATGTCCC ACAGTGGCAC CTGCTCCCTC ATTAACCAAA
    CCCCAGATGT TTGAAGCTGT GGCCAGAGTG CACGGGTCCC CAGTGGAGCT CAAACAGGAC
    AACAATAGCA TTGAGATCAA CATAAAGAAG CCCAGTTCCG TTCCCCAAGA ACTGAGAGCA
    ACCACTGAGG AAACTGAACC CAACAGTCAA GAGGATGAAA ATGATGGTGG AAAAACACAG
    AAAGGAAATA CAGAACTCGC CCCATCAGAG CCACAGCATT TCACGACAAC TGTGACTCGG
    TCCAGCCCGA CTGTGCCCTT TGTAGAGTTT CCCTCCAGCC CCCAGCGGAG GAACGATGTG
    CCACCAGAAG AAGACCAGAA GAAACTAGGA AATGAAATGA GTGGGAAGGT GGAGTTGGTG
    CTGTCCCAAA AGGTGGTAAA GCCAAAATCA CCAGAACCTG AAGCAACCCT GACATATCCA
    TTTCTGGAAA AAATGTCTGA AACCAACCAA TTACACTTGC CAAATCTCAA TTCTCAAGGG
    TCGCCTGGCG CTGCCGGCTT CCCGCTCAGA GTTCAGAACT CTTGGCGGCG CTCCCAGTTT
    TTCTCGCAGT CAGTGGATTC TCCAGGCAGT GAAAAGTCTC CTGTAACTAC TCCTTTCAAG
    TTTTGGGCGT GGGACCCAGA AGAGGAGCGC CGGCGACAGG AAAAGTGGCA ACAGGAGCAG
    GAACGTTTGC TCCAGGAGAG ATACCAGAAG GAACAGAACA AGCTGAAGGA AGAGTGGGAA
    AAGGCCCAGA AGGAGGTAGA GGAAGAGGAA CGCAGATACT ACGAGGAGGA GCGCAAAATA
    ATTGAAGACA CTGTGGTTCC ATTTACTCTT TCTTCAAGTT CTGCAGACCA GCTGTCTACG
    TCCTCCTCTG TGACCGAGGG CACCGGGACA GTGAGTAAGA TGGACTTGGA AAATTGTCGG
    GAGGAAGAAC AGGAGACAAA ACAGAAGAAG CCACTCCGGG GGGACGACAG TGATTTAATG
    CCTAAGACTA GGGAAGATCA CCCCCTGGAG GGAAAGGGCA GCCTAACTCA AGGGGCCTTG
    GCTCCTTCTA AGAATCCTGG ATCGAAAGGA GTCCATCAGG ACCATCAGCT GGATGCAGAA
    ACGGGGGCCC TCCGCTGCGG GATGAACTCC CAGCTAGCTC AGGATCCACC CCAGAATACG
    CAGGTATCAA ACCCACCAAT GCACGTTTTA GAAGATGTGA AGCCAAAAAC GCTCCCTTTG
    GATAAAAGCA TTAACCATCA GATTGAGTCA CCGAGTGAGA GACGGAAGAA AAGCCCTCGA
    GAGAATTTCC AGGCCGGGCA CTTGTCCCCC TGCTCTCCCA CCCCTCCTGG CCAATCACCA
    AACAGGTCGA TCAGCGGGAA GAAGCTGTGC TCGTCCTGTG GGCTCCCCCT GGGGAAGGGA
    GCGGCCATGA TCATCGAGAC GCTGCAGCTC TACTTCCACA TCCAGTGCTT TCGGTGTGGA
    CTCTGCAAAG GACAACTTGG GGACGCGGTG AGCGGGACAG ACGTCAGGAT CCGCAGTGGC
    CTGCTCAACT GTAACGACTG CTACCTGCGA TCCAGAAGTG CCGGCCAGCC CACCACCCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39789
    Clone ID Related Accession (Same CDS sequence) XM_014118778.1
    Accession Version XM_014118778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGGGTCG CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT
    CAGAACTCTT GGCGGCGCTC CCAGTTTTTC TCGCAGTCAG TGGATTCTCC AGGCAGTGAA
    AAGTCTCCTG TAACTACTCC TTTCAAGTTT TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG
    CGACAGGAAA AGTGGCAACA GGAGCAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAA
    CAGAACAAGC TGAAGGAAGA GTGGGAAAAG GCCCAGAAGG AGGTAGAGGA AGAGGAACGC
    AGATACTACG AGGAGGAGCG CAAAATAATT GAAGACACTG TGGTTCCATT TACTCTTTCT
    TCAAGTTCTG CAGACCAGCT GTCTACGTCC TCCTCTGTGA CCGAGGGCAC CGGGACAGTG
    AGTAAGATGG ACTTGGAAAA TTGTCGGGAG GAAGAACAGG AGACAAAACA GAAGAAGCCA
    CTCCGGGGGG ACGACAGTGA TTTAATGCCT AAGACTAGGG AAGATCACCC CCTGGAGGGA
    AAGGGCAGCC TAACTCAAGG GGCCTTGGCT CCTTCTAAGA ATCCTGGATC GAAAGGAGTC
    CATCAGGACC ATCAGCTGGA TGCAGAAACG GGGGCCCTCC GCTGCGGGAT GAACTCCCAG
    CTAGCTCAGG ATCCACCCCA GAATACGCAG GTATCAAACC CACCAATGCA CGTTTTAGAA
    GATGTGAAGC CAAAAACGCT CCCTTTGGAT AAAAGCATTA ACCATCAGAT TGAGTCACCG
    AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG AATTTCCAGG CCGGGCACTT GTCCCCCTGC
    TCTCCCACCC CTCCTGGCCA ATCACCAAAC AGGTCGATCA GCGGGAAGAA GCTGTGCTCG
    TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG GCCATGATCA TCGAGACGCT GCAGCTCTAC
    TTCCACATCC AGTGCTTTCG GTGTGGACTC TGCAAAGGAC AACTTGGGGA CGCGGTGAGC
    GGGACAGACG TCAGGATCCG CAGTGGCCTG CTCAACTGTA ACGACTGCTA CCTGCGATCC
    AGAAGTGCCG GCCAGCCCAC CACCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974253.1
    CDS16..3285
    Translation

    Target ORF information:

    RefSeq Version XM_014118778.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGGGTCG CCTGGCGCTG CCGGCTTCCC GCTCAGAGTT
    CAGAACTCTT GGCGGCGCTC CCAGTTTTTC TCGCAGTCAG TGGATTCTCC AGGCAGTGAA
    AAGTCTCCTG TAACTACTCC TTTCAAGTTT TGGGCGTGGG ACCCAGAAGA GGAGCGCCGG
    CGACAGGAAA AGTGGCAACA GGAGCAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAA
    CAGAACAAGC TGAAGGAAGA GTGGGAAAAG GCCCAGAAGG AGGTAGAGGA AGAGGAACGC
    AGATACTACG AGGAGGAGCG CAAAATAATT GAAGACACTG TGGTTCCATT TACTCTTTCT
    TCAAGTTCTG CAGACCAGCT GTCTACGTCC TCCTCTGTGA CCGAGGGCAC CGGGACAGTG
    AGTAAGATGG ACTTGGAAAA TTGTCGGGAG GAAGAACAGG AGACAAAACA GAAGAAGCCA
    CTCCGGGGGG ACGACAGTGA TTTAATGCCT AAGACTAGGG AAGATCACCC CCTGGAGGGA
    AAGGGCAGCC TAACTCAAGG GGCCTTGGCT CCTTCTAAGA ATCCTGGATC GAAAGGAGTC
    CATCAGGACC ATCAGCTGGA TGCAGAAACG GGGGCCCTCC GCTGCGGGAT GAACTCCCAG
    CTAGCTCAGG ATCCACCCCA GAATACGCAG GTATCAAACC CACCAATGCA CGTTTTAGAA
    GATGTGAAGC CAAAAACGCT CCCTTTGGAT AAAAGCATTA ACCATCAGAT TGAGTCACCG
    AGTGAGAGAC GGAAGAAAAG CCCTCGAGAG AATTTCCAGG CCGGGCACTT GTCCCCCTGC
    TCTCCCACCC CTCCTGGCCA ATCACCAAAC AGGTCGATCA GCGGGAAGAA GCTGTGCTCG
    TCCTGTGGGC TCCCCCTGGG GAAGGGAGCG GCCATGATCA TCGAGACGCT GCAGCTCTAC
    TTCCACATCC AGTGCTTTCG GTGTGGACTC TGCAAAGGAC AACTTGGGGA CGCGGTGAGC
    GGGACAGACG TCAGGATCCG CAGTGGCCTG CTCAACTGTA ACGACTGCTA CCTGCGATCC
    AGAAGTGCCG GCCAGCCCAC CACCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39790
    Clone ID Related Accession (Same CDS sequence) XM_014118779.1
    Accession Version XM_014118779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGA AAAGCCCTCG AGAGAATTTC
    CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC ACCCCTCCTG GCCAATCACC AAACAGGTCG
    ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT GGGCTCCCCC TGGGGAAGGG AGCGGCCATG
    ATCATCGAGA CGCTGCAGCT CTACTTCCAC ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA
    GGACAACTTG GGGACGCGGT GAGCGGGACA GACGTCAGGA TCCGCAGTGG CCTGCTCAAC
    TGTAACGACT GCTACCTGCG ATCCAGAAGT GCCGGCCAGC CCACCACCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974254.1
    CDS16..3249
    Translation

    Target ORF information:

    RefSeq Version XM_014118779.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGA AAAGCCCTCG AGAGAATTTC
    CAGGCCGGGC ACTTGTCCCC CTGCTCTCCC ACCCCTCCTG GCCAATCACC AAACAGGTCG
    ATCAGCGGGA AGAAGCTGTG CTCGTCCTGT GGGCTCCCCC TGGGGAAGGG AGCGGCCATG
    ATCATCGAGA CGCTGCAGCT CTACTTCCAC ATCCAGTGCT TTCGGTGTGG ACTCTGCAAA
    GGACAACTTG GGGACGCGGT GAGCGGGACA GACGTCAGGA TCCGCAGTGG CCTGCTCAAC
    TGTAACGACT GCTACCTGCG ATCCAGAAGT GCCGGCCAGC CCACCACCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39791
    Clone ID Related Accession (Same CDS sequence) XM_014118780.1
    Accession Version XM_014118780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAGGCA GTGAAAAGTC TCCTGTAACT
    ACTCCTTTCA AGTTTTGGGC GTGGGACCCA GAAGAGGAGC GCCGGCGACA GGAAAAGTGG
    CAACAGGAGC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAACAGAA CAAGCTGAAG
    GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA GAGGAAGAGG AACGCAGATA CTACGAGGAG
    GAGCGCAAAA TAATTGAAGA CACTGTGGTT CCATTTACTC TTTCTTCAAG TTCTGCAGAC
    CAGCTGTCTA CGTCCTCCTC TGTGACCGAG GGCACCGGGA CAGTGAGTAA GATGGACTTG
    GAAAATTGTC GGGAGGAAGA ACAGGAGACA AAACAGAAGA AGCCACTCCG GGGGGACGAC
    AGTGATTTAA TGCCTAAGAC TAGGGAAGAT CACCCCCTGG AGGGAAAGGG CAGCCTAACT
    CAAGGGGCCT TGGCTCCTTC TAAGAATCCT GGATCGAAAG GAGTCCATCA GGACCATCAG
    CTGGATGCAG AAACGGGGGC CCTCCGCTGC GGGATGAACT CCCAGCTAGC TCAGGATCCA
    CCCCAGAATA CGCAGGTATC AAACCCACCA ATGCACGTTT TAGAAGATGT GAAGCCAAAA
    ACGCTCCCTT TGGATAAAAG CATTAACCAT CAGATTGAGT CACCGAGTGA GAGACGGAAG
    AAAAGCCCTC GAGAGAATTT CCAGGCCGGG CACTTGTCCC CCTGCTCTCC CACCCCTCCT
    GGCCAATCAC CAAACAGGTC GATCAGCGGG AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC
    CTGGGGAAGG GAGCGGCCAT GATCATCGAG ACGCTGCAGC TCTACTTCCA CATCCAGTGC
    TTTCGGTGTG GACTCTGCAA AGGACAACTT GGGGACGCGG TGAGCGGGAC AGACGTCAGG
    ATCCGCAGTG GCCTGCTCAA CTGTAACGAC TGCTACCTGC GATCCAGAAG TGCCGGCCAG
    CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974255.1
    CDS16..3210
    Translation

    Target ORF information:

    RefSeq Version XM_014118780.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAGGCA GTGAAAAGTC TCCTGTAACT
    ACTCCTTTCA AGTTTTGGGC GTGGGACCCA GAAGAGGAGC GCCGGCGACA GGAAAAGTGG
    CAACAGGAGC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAACAGAA CAAGCTGAAG
    GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA GAGGAAGAGG AACGCAGATA CTACGAGGAG
    GAGCGCAAAA TAATTGAAGA CACTGTGGTT CCATTTACTC TTTCTTCAAG TTCTGCAGAC
    CAGCTGTCTA CGTCCTCCTC TGTGACCGAG GGCACCGGGA CAGTGAGTAA GATGGACTTG
    GAAAATTGTC GGGAGGAAGA ACAGGAGACA AAACAGAAGA AGCCACTCCG GGGGGACGAC
    AGTGATTTAA TGCCTAAGAC TAGGGAAGAT CACCCCCTGG AGGGAAAGGG CAGCCTAACT
    CAAGGGGCCT TGGCTCCTTC TAAGAATCCT GGATCGAAAG GAGTCCATCA GGACCATCAG
    CTGGATGCAG AAACGGGGGC CCTCCGCTGC GGGATGAACT CCCAGCTAGC TCAGGATCCA
    CCCCAGAATA CGCAGGTATC AAACCCACCA ATGCACGTTT TAGAAGATGT GAAGCCAAAA
    ACGCTCCCTT TGGATAAAAG CATTAACCAT CAGATTGAGT CACCGAGTGA GAGACGGAAG
    AAAAGCCCTC GAGAGAATTT CCAGGCCGGG CACTTGTCCC CCTGCTCTCC CACCCCTCCT
    GGCCAATCAC CAAACAGGTC GATCAGCGGG AAGAAGCTGT GCTCGTCCTG TGGGCTCCCC
    CTGGGGAAGG GAGCGGCCAT GATCATCGAG ACGCTGCAGC TCTACTTCCA CATCCAGTGC
    TTTCGGTGTG GACTCTGCAA AGGACAACTT GGGGACGCGG TGAGCGGGAC AGACGTCAGG
    ATCCGCAGTG GCCTGCTCAA CTGTAACGAC TGCTACCTGC GATCCAGAAG TGCCGGCCAG
    CCCACCACCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39792
    Clone ID Related Accession (Same CDS sequence) XM_014118781.1
    Accession Version XM_014118781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGT CGATCAGCGG GAAGAAGCTG
    TGCTCGTCCT GTGGGCTCCC CCTGGGGAAG GGAGCGGCCA TGATCATCGA GACGCTGCAG
    CTCTACTTCC ACATCCAGTG CTTTCGGTGT GGACTCTGCA AAGGACAACT TGGGGACGCG
    GTGAGCGGGA CAGACGTCAG GATCCGCAGT GGCCTGCTCA ACTGTAACGA CTGCTACCTG
    CGATCCAGAA GTGCCGGCCA GCCCACCACC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974256.1
    CDS16..3171
    Translation

    Target ORF information:

    RefSeq Version XM_014118781.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGG GTCGCCTGGC
    GCTGCCGGCT TCCCGCTCAG AGTTCAGAAC TCTTGGCGGC GCTCCCAGTT TTTCTCGCAG
    TCAGTGGATT CTCCAGGCAG TGAAAAGTCT CCTGTAACTA CTCCTTTCAA GTTTTGGGCG
    TGGGACCCAG AAGAGGAGCG CCGGCGACAG GAAAAGTGGC AACAGGAGCA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAACAGAAC AAGCTGAAGG AAGAGTGGGA AAAGGCCCAG
    AAGGAGGTAG AGGAAGAGGA ACGCAGATAC TACGAGGAGG AGCGCAAAAT AATTGAAGAC
    ACTGTGGTTC CATTTACTCT TTCTTCAAGT TCTGCAGACC AGCTGTCTAC GTCCTCCTCT
    GTGACCGAGG GCACCGGGAC AGTGAGTAAG ATGGACTTGG AAAATTGTCG GGAGGAAGAA
    CAGGAGACAA AACAGAAGAA GCCACTCCGG GGGGACGACA GTGATTTAAT GCCTAAGACT
    AGGGAAGATC ACCCCCTGGA GGGAAAGGGC AGCCTAACTC AAGGGGCCTT GGCTCCTTCT
    AAGAATCCTG GATCGAAAGG AGTCCATCAG GACCATCAGC TGGATGCAGA AACGGGGGCC
    CTCCGCTGCG GGATGAACTC CCAGCTAGCT CAGGATCCAC CCCAGAATAC GCAGGTATCA
    AACCCACCAA TGCACGTTTT AGAAGATGTG AAGCCAAAAA CGCTCCCTTT GGATAAAAGC
    ATTAACCATC AGATTGAGTC ACCGAGTGAG AGACGGAAGT CGATCAGCGG GAAGAAGCTG
    TGCTCGTCCT GTGGGCTCCC CCTGGGGAAG GGAGCGGCCA TGATCATCGA GACGCTGCAG
    CTCTACTTCC ACATCCAGTG CTTTCGGTGT GGACTCTGCA AAGGACAACT TGGGGACGCG
    GTGAGCGGGA CAGACGTCAG GATCCGCAGT GGCCTGCTCA ACTGTAACGA CTGCTACCTG
    CGATCCAGAA GTGCCGGCCA GCCCACCACC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39793
    Clone ID Related Accession (Same CDS sequence) XM_014118782.1
    Accession Version XM_014118782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAGGCA GTGAAAAGTC TCCTGTAACT
    ACTCCTTTCA AGTTTTGGGC GTGGGACCCA GAAGAGGAGC GCCGGCGACA GGAAAAGTGG
    CAACAGGAGC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAACAGAA CAAGCTGAAG
    GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA GAGGAAGAGG AACGCAGATA CTACGAGGAG
    GAGCGCAAAA TAATTGAAGA CACTGTGGTT CCATTTACTC TTTCTTCAAG TTCTGCAGAC
    CAGCTGTCTA CGTCCTCCTC TGTGACCGAG GGCACCGGGA CAGTGAGTAA GATGGACTTG
    GAAAATTGTC GGGAGGAAGA ACAGGAGACA AAACAGAAGA AGCCACTCCG GGGGGACGAC
    AGTGATTTAA TGCCTAAGAC TAGGGAAGAT CACCCCCTGG AGGGAAAGGG CAGCCTAACT
    CAAGGGGCCT TGGCTCCTTC TAAGAATCCT GGATCGAAAG GAGTCCATCA GGACCATCAG
    CTGGATGCAG AAACGGGGGC CCTCCGCTGC GGGATGAACT CCCAGCTAGC TCAGGATCCA
    CCCCAGAATA CGCAGGTATC AAACCCACCA ATGCACGTTT TAGAAGATGT GAAGCCAAAA
    ACGCTCCCTT TGGATAAAAG CATTAACCAT CAGATTGAGT CACCGAGTGA GAGACGGAAG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AGTGCCGGCC AGCCCACCAC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974257.1
    CDS16..3132
    Translation

    Target ORF information:

    RefSeq Version XM_014118782.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGAT ACGGGCCGAG GACTCCTGTG
    TCTGATGACG CAGAGAGCTC CAGCCTGCTG GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCACACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTCA TAAATAGCCA GCTGCGCGAG
    GAGGACGACA AGTGGCAGGA CGACCTGGCT CGTTGGAAGA GCCGTAGAAG AAGTGCTTCC
    CAGGACTTAA TCAAGAAAGA GGAAGAGAGG AAGAAAATGG AGAAGTTACT GGCTGGAGAG
    GATGGGACGA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATCGT TCAAGAAAAG
    GAGCGGAGAG AGAGGGAGCT GCATGAGGCC TATAAGAACG CGCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAGCAGTA CATCGAGAGG TTCACCATCA GCGAGGCTGT CCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACGG AGCCAAACTT AACCCCCTTC
    CTGAACGACC CTAACCCCAT GAAGTACCTG CGGCGACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACCG TGGAGACCAC CATCGCCCGG ACCAGCGTTC TGGATACCGG CGTGTCAGCA
    GGCGGCGGGT CTCCAAGCAA GACTGTCCCT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA CTCTCAAGAC GTTCTGAAGA CCTTTAAGGT AGATGGGAAA
    GTCAGCATGA ATGGAGAAGC AGTTCACGGT GACAAGGAGA AGGAGAGAGA ATGTCCCACA
    GTGGCACCTG CTCCCTCATT AACCAAACCC CAGATGTTTG AAGCTGTGGC CAGAGTGCAC
    GGGTCCCCAG TGGAGCTCAA ACAGGACAAC AATAGCATTG AGATCAACAT AAAGAAGCCC
    AGTTCCGTTC CCCAAGAACT GAGAGCAACC ACTGAGGAAA CTGAACCCAA CAGTCAAGAG
    GATGAAAATG ATGGTGGAAA AACACAGAAA GGAAATACAG AACTCGCCCC ATCAGAGCCA
    CAGCATTTCA CGACAACTGT GACTCGGTCC AGCCCGACTG TGCCCTTTGT AGAGTTTCCC
    TCCAGCCCCC AGCGGAGGAA CGATGTGCCA CCAGAAGAAG ACCAGAAGAA ACTAGGAAAT
    GAAATGAGTG GGAAGGTGGA GTTGGTGCTG TCCCAAAAGG TGGTAAAGCC AAAATCACCA
    GAACCTGAAG CAACCCTGAC ATATCCATTT CTGGAAAAAA TGTCTGAAAC CAACCAATTA
    CACTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAGGCA GTGAAAAGTC TCCTGTAACT
    ACTCCTTTCA AGTTTTGGGC GTGGGACCCA GAAGAGGAGC GCCGGCGACA GGAAAAGTGG
    CAACAGGAGC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAACAGAA CAAGCTGAAG
    GAAGAGTGGG AAAAGGCCCA GAAGGAGGTA GAGGAAGAGG AACGCAGATA CTACGAGGAG
    GAGCGCAAAA TAATTGAAGA CACTGTGGTT CCATTTACTC TTTCTTCAAG TTCTGCAGAC
    CAGCTGTCTA CGTCCTCCTC TGTGACCGAG GGCACCGGGA CAGTGAGTAA GATGGACTTG
    GAAAATTGTC GGGAGGAAGA ACAGGAGACA AAACAGAAGA AGCCACTCCG GGGGGACGAC
    AGTGATTTAA TGCCTAAGAC TAGGGAAGAT CACCCCCTGG AGGGAAAGGG CAGCCTAACT
    CAAGGGGCCT TGGCTCCTTC TAAGAATCCT GGATCGAAAG GAGTCCATCA GGACCATCAG
    CTGGATGCAG AAACGGGGGC CCTCCGCTGC GGGATGAACT CCCAGCTAGC TCAGGATCCA
    CCCCAGAATA CGCAGGTATC AAACCCACCA ATGCACGTTT TAGAAGATGT GAAGCCAAAA
    ACGCTCCCTT TGGATAAAAG CATTAACCAT CAGATTGAGT CACCGAGTGA GAGACGGAAG
    TCGATCAGCG GGAAGAAGCT GTGCTCGTCC TGTGGGCTCC CCCTGGGGAA GGGAGCGGCC
    ATGATCATCG AGACGCTGCA GCTCTACTTC CACATCCAGT GCTTTCGGTG TGGACTCTGC
    AAAGGACAAC TTGGGGACGC GGTGAGCGGG ACAGACGTCA GGATCCGCAG TGGCCTGCTC
    AACTGTAACG ACTGCTACCT GCGATCCAGA AGTGCCGGCC AGCCCACCAC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39794
    Clone ID Related Accession (Same CDS sequence) XM_014118783.1
    Accession Version XM_014118783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LIM and calponin homology domains-containing protein 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA
    GGCAGTGAAA AGTCTCCTGT AACTACTCCT TTCAAGTTTT GGGCGTGGGA CCCAGAAGAG
    GAGCGCCGGC GACAGGAAAA GTGGCAACAG GAGCAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAAC AGAACAAGCT GAAGGAAGAG TGGGAAAAGG CCCAGAAGGA GGTAGAGGAA
    GAGGAACGCA GATACTACGA GGAGGAGCGC AAAATAATTG AAGACACTGT GGTTCCATTT
    ACTCTTTCTT CAAGTTCTGC AGACCAGCTG TCTACGTCCT CCTCTGTGAC CGAGGGCACC
    GGGACAGTGA GTAAGATGGA CTTGGAAAAT TGTCGGGAGG AAGAACAGGA GACAAAACAG
    AAGAAGCCAC TCCGGGGGGA CGACAGTGAT TTAATGCCTA AGACTAGGGA AGATCACCCC
    CTGGAGGGAA AGGGCAGCCT AACTCAAGGG GCCTTGGCTC CTTCTAAGAA TCCTGGATCG
    AAAGGAGTCC ATCAGGACCA TCAGCTGGAT GCAGAAACGG GGGCCCTCCG CTGCGGGATG
    AACTCCCAGC TAGCTCAGGA TCCACCCCAG AATACGCAGG TATCAAACCC ACCAATGCAC
    GTTTTAGAAG ATGTGAAGCC AAAAACGCTC CCTTTGGATA AAAGCATTAA CCATCAGATT
    GAGTCACCGA GTGAGAGACG GAAGTCGATC AGCGGGAAGA AGCTGTGCTC GTCCTGTGGG
    CTCCCCCTGG GGAAGGGAGC GGCCATGATC ATCGAGACGC TGCAGCTCTA CTTCCACATC
    CAGTGCTTTC GGTGTGGACT CTGCAAAGGA CAACTTGGGG ACGCGGTGAG CGGGACAGAC
    GTCAGGATCC GCAGTGGCCT GCTCAACTGT AACGACTGCT ACCTGCGATC CAGAAGTGCC
    GGCCAGCCCA CCACCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974258.1
    CDS16..3096
    Translation

    Target ORF information:

    RefSeq Version XM_014118783.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGATGG CACCGCCCTG GAGAGCGGAA GCAGCGTTGC AAGTAACCGG CAGAAGTTTT 
    GGTGACAAAG ATTTTCGGAC AGGGTTAGAA AATGGAATCC TCCTCTGCGA GTTGCTGAAT
    GCTATAAAGC CAGGACTTGT TAAAAAGATC AATAGATTGC CTACCCCCAT TGCGGGATTG
    GACAATATCA TCTTATTCTT GAGAGGTTGT AAAGAGCTTG GCCTGAGGGA ATCTCAACTT
    TTTGACCCTA GTGACCTACA GGATACATCC AACAGAGTGA CCGTCAAGAG CCTCGATTAC
    AGTAGGAAAC TGAAAAATGT ATTAGTTACC ATTTACTGGC TGGGAAAAGC AGCAAATAGC
    TCTGCGTCCT ACAGCGGAAC GACACTGAAC CTGAAGGAGT TCGAAGGCTT GCTGGCTCAG
    ATGAGAAAGG AGACCGATGA CATCGAGAGC CCTAAGCGCA GTATCCGGGA CAGCGGCTAC
    ATAGACTGCT GGGACTCCGA GCGCAGCGAC TCACTGTCCC CACCTCGCCA CGGCCGAGAT
    GATTCCTTCG ACAGCCTGGA CTCCTTCGGG TCCCGATCGC GGCAGACGCC CTCGCCAGAT
    GTTGTCCTCC GGGGAAGCAG CGACGGGAGG GGAAGCGACT CTGAGTCTGA CCTGCCGCAC
    CGCAAGCTGC CGGACGTGAA GAAGGATGAC ATGTCTGCGA GGCGGACGTC CCACGGCGAG
    CCCAAGTCGG CGGTGCCGTT CAACCAGTAC CTCCCGAACA AAAGCAACCA GACGGCCTAC
    GTGCCGGCGC CTCTGAGGAG GAGGAAGGCT GAGCGGGAGG AGTACCGGAA GAGCTGGAGC
    ACCGCCACCT CGCCCCTGGG CGGGGACCGG CCCTGCAGGA GCTCCAGCCT GCTGGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCA CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTCATAAATA GCCAGCTGCG CGAGGAGGAC GACAAGTGGC AGGACGACCT GGCTCGTTGG
    AAGAGCCGTA GAAGAAGTGC TTCCCAGGAC TTAATCAAGA AAGAGGAAGA GAGGAAGAAA
    ATGGAGAAGT TACTGGCTGG AGAGGATGGG ACGAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TCGTTCAAGA AAAGGAGCGG AGAGAGAGGG AGCTGCATGA GGCCTATAAG
    AACGCGCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAGC AGTACATCGA GAGGTTCACC
    ATCAGCGAGG CTGTCCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACGGAGCCAA ACTTAACCCC CTTCCTGAAC GACCCTAACC CCATGAAGTA CCTGCGGCGA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACCGTGGAGA CCACCATCGC CCGGACCAGC
    GTTCTGGATA CCGGCGTGTC AGCAGGCGGC GGGTCTCCAA GCAAGACTGT CCCTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAACTCTCA AGACGTTCTG
    AAGACCTTTA AGGTAGATGG GAAAGTCAGC ATGAATGGAG AAGCAGTTCA CGGTGACAAG
    GAGAAGGAGA GAGAATGTCC CACAGTGGCA CCTGCTCCCT CATTAACCAA ACCCCAGATG
    TTTGAAGCTG TGGCCAGAGT GCACGGGTCC CCAGTGGAGC TCAAACAGGA CAACAATAGC
    ATTGAGATCA ACATAAAGAA GCCCAGTTCC GTTCCCCAAG AACTGAGAGC AACCACTGAG
    GAAACTGAAC CCAACAGTCA AGAGGATGAA AATGATGGTG GAAAAACACA GAAAGGAAAT
    ACAGAACTCG CCCCATCAGA GCCACAGCAT TTCACGACAA CTGTGACTCG GTCCAGCCCG
    ACTGTGCCCT TTGTAGAGTT TCCCTCCAGC CCCCAGCGGA GGAACGATGT GCCACCAGAA
    GAAGACCAGA AGAAACTAGG AAATGAAATG AGTGGGAAGG TGGAGTTGGT GCTGTCCCAA
    AAGGTGGTAA AGCCAAAATC ACCAGAACCT GAAGCAACCC TGACATATCC ATTTCTGGAA
    AAAATGTCTG AAACCAACCA ATTACACTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA
    GGCAGTGAAA AGTCTCCTGT AACTACTCCT TTCAAGTTTT GGGCGTGGGA CCCAGAAGAG
    GAGCGCCGGC GACAGGAAAA GTGGCAACAG GAGCAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAAC AGAACAAGCT GAAGGAAGAG TGGGAAAAGG CCCAGAAGGA GGTAGAGGAA
    GAGGAACGCA GATACTACGA GGAGGAGCGC AAAATAATTG AAGACACTGT GGTTCCATTT
    ACTCTTTCTT CAAGTTCTGC AGACCAGCTG TCTACGTCCT CCTCTGTGAC CGAGGGCACC
    GGGACAGTGA GTAAGATGGA CTTGGAAAAT TGTCGGGAGG AAGAACAGGA GACAAAACAG
    AAGAAGCCAC TCCGGGGGGA CGACAGTGAT TTAATGCCTA AGACTAGGGA AGATCACCCC
    CTGGAGGGAA AGGGCAGCCT AACTCAAGGG GCCTTGGCTC CTTCTAAGAA TCCTGGATCG
    AAAGGAGTCC ATCAGGACCA TCAGCTGGAT GCAGAAACGG GGGCCCTCCG CTGCGGGATG
    AACTCCCAGC TAGCTCAGGA TCCACCCCAG AATACGCAGG TATCAAACCC ACCAATGCAC
    GTTTTAGAAG ATGTGAAGCC AAAAACGCTC CCTTTGGATA AAAGCATTAA CCATCAGATT
    GAGTCACCGA GTGAGAGACG GAAGTCGATC AGCGGGAAGA AGCTGTGCTC GTCCTGTGGG
    CTCCCCCTGG GGAAGGGAGC GGCCATGATC ATCGAGACGC TGCAGCTCTA CTTCCACATC
    CAGTGCTTTC GGTGTGGACT CTGCAAAGGA CAACTTGGGG ACGCGGTGAG CGGGACAGAC
    GTCAGGATCC GCAGTGGCCT GCTCAACTGT AACGACTGCT ACCTGCGATC CAGAAGTGCC
    GGCCAGCCCA CCACCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.