limch1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following limch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the limch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa49695 XM_004911217.4
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49696 XM_004911218.4
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49697 XM_012955696.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49698 XM_012955698.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49699 XM_004911221.4
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49699 XM_004911220.4
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49700 XM_031895385.1
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49701 XM_012955699.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49702 XM_031895386.1
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa04746 XM_004911217.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa04747 XM_004911218.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa04749 XM_004911221.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa04749 XM_004911220.3
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa30867 XM_012955695.2
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa30868 XM_012955696.2
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa30869 XM_012955698.2
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa30870 XM_012955699.2
Latest version!
Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa49695
    Clone ID Related Accession (Same CDS sequence) XM_004911217.4
    Accession Version XM_004911217.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911217.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911274.2
    CDS491..6265
    Translation

    Target ORF information:

    RefSeq Version XM_004911217.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911217.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49696
    Clone ID Related Accession (Same CDS sequence) XM_004911218.4
    Accession Version XM_004911218.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911218.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC
    CCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911275.2
    CDS491..6259
    Translation

    Target ORF information:

    RefSeq Version XM_004911218.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911218.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC
    CCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49697
    Clone ID Related Accession (Same CDS sequence) XM_012955696.3
    Accession Version XM_012955696.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT
    GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA
    CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG
    CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG
    AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT
    CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT
    ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC
    AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT
    ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG
    TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG
    TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG
    TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA
    GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT
    ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT
    GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT
    GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA
    CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT
    GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811150.2
    CDS491..6184
    Translation

    Target ORF information:

    RefSeq Version XM_012955696.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955696.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT
    GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA
    CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG
    CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG
    AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT
    CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT
    ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC
    AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT
    ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG
    TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG
    TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG
    TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA
    GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT
    ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT
    GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT
    GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA
    CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT
    GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49698
    Clone ID Related Accession (Same CDS sequence) XM_012955698.3
    Accession Version XM_012955698.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT
    GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC
    AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG
    AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG
    TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA
    CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT
    AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA
    AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG
    AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT
    TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG
    TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT
    CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG
    ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG
    AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811152.2
    CDS491..6172
    Translation

    Target ORF information:

    RefSeq Version XM_012955698.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955698.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT
    GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC
    AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG
    AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG
    TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA
    CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT
    AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA
    AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG
    AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT
    TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG
    TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT
    CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG
    ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG
    AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49699
    Clone ID Related Accession (Same CDS sequence) XM_004911220.4 , XM_004911221.4
    Accession Version XM_004911220.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911220.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911277.2
    CDS491..6094
    Translation

    Target ORF information:

    RefSeq Version XM_004911220.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911220.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49699
    Clone ID Related Accession (Same CDS sequence) XM_004911220.4 , XM_004911221.4
    Accession Version XM_004911221.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911278.2
    CDS491..6094
    Translation

    Target ORF information:

    RefSeq Version XM_004911221.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911221.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49700
    Clone ID Related Accession (Same CDS sequence) XM_031895385.1
    Accession Version XM_031895385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751245.1
    CDS491..6025
    Translation

    Target ORF information:

    RefSeq Version XM_031895385.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49701
    Clone ID Related Accession (Same CDS sequence) XM_012955699.3
    Accession Version XM_012955699.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA
    TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT
    CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG
    ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA
    GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA
    AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA
    GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC
    TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA
    AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG
    ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT
    GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA
    AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT
    GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT
    TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA
    TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA
    AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG
    CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG
    GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC
    CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT
    GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA
    AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC
    CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG
    TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA
    GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT
    GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT
    TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA
    TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC
    CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC
    AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA
    GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA
    AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT
    TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT
    TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA
    CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG
    ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG
    GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA
    GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT
    GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA
    GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT
    ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG
    AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA
    AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC
    CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG
    GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT
    TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811153.2
    CDS491..5998
    Translation

    Target ORF information:

    RefSeq Version XM_012955699.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955699.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA
    TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT
    CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG
    ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA
    GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA
    AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA
    GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC
    TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA
    AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG
    ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT
    GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA
    AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT
    GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT
    TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA
    TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA
    AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG
    CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG
    GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC
    CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT
    GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA
    AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC
    CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG
    TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA
    GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT
    GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT
    TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA
    TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC
    CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC
    AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA
    GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA
    AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT
    TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT
    TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA
    CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG
    ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG
    GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA
    GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT
    GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA
    GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT
    ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG
    AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA
    AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC
    CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG
    GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT
    TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49702
    Clone ID Related Accession (Same CDS sequence) XM_031895386.1
    Accession Version XM_031895386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGAAAAG AAAATGAGGA AATAGAGAGT CCAAAACGCA GCATACGGGA CAGTGGCTAC 
    ATAGATTGTT GGGACTCTGA GAGAAGTGAC TCCCTTTCTC CTCCCAGACA TGGTAGAGAT
    GACTCCTTCG ATAGTTTAGA CTCATTTGGA TCTAGATCAC AACAGACACC ATCTCCAGAT
    GTAGTGCTGA GGGGAAGCAG CGACGGCAGA GGAAGCGACT CTGAGTCTGA CCTACCTCAC
    AGAAGGTTAC CTGATGTGCG AAAAGATGAT ATGTCTGCTA GACGGGTATC CTACGGTGAA
    AACAAAGCAT TTGTGCCTTT CAACCAATAC CTCCCCAACA AGAGCAACCA GACCTCCTAC
    ATCCCGGCAC CCCTGAGGAA GAAGAAGGCT GAGCGTGAGG ACTACCGGAA AAGCTGGAGC
    ACAGCTACAT CCCCCCTGGG GGGAGAGCGA CCCTTCAGTC CCAAACAAAG TCCAGTCATT
    AAAGAAGAAC AAAACGAGTT GCAGTGTGAA GGAGAAGGGA CCAAGCAGAT GATTGCCTTA
    AAAGAACCTT GCCTTGGAGC AGAAAATAGG AGTGAGAGAG TTGGTGAAAG TGAAGGGGGT
    GTCATGGGAC AATCATCTGC TAATGATGAC ATTCCAAAAG ATGCAGAGGA AGTGAGAAAG
    CGCAAGCACC TTCAGAAAGC GGGTATCAAG GTCTTGCCCG CAACAAAGCG CTACAGCAGT
    CCAAGTCAAT TTGCTGAAGA CATTGATGGC GCTCCAGACA TTGTGCTGCG AAAAGAGAAC
    ACCTTCCTGA AGTCATATGA AGGGATGGGC TCTGATTCCG AAGAAGAAGA AGAGTGCAAA
    GTACCTGATA TACAGCGAGA TGACTTGGCC ACAAGGCGTG CAAGGATGAA CCAACCAAAA
    AGCCCAATGC AGTTTAATCA GTATATACAG GGGTCATACT TAAACAAAAA CAGAGCATCA
    AGAAAGCTGA ACAGTTCTAA GACTTCCACT CCAGCTTCTC CAAAAATGTC TCAAGATATC
    AGGGAGTACC AAGGCCCCCA GTTATTAAAG CCATCACACG TCTGCAAAGA AAAGACATTT
    CCAATACCCT CAGACAATAA AGAGGATGCA GAAGCAGAGC AAGAAAAAGT TCCCGACATT
    CAGAAAGACG ACCTGGCGAA ACGCAAAGCT AAAGCTTCAG TTTCACTGCC AAAGGAACCA
    TCGGCCTTTG CAGCGGTTTC AATTACAAAG GCCGACATGG AAACTTGGCA GAGGCTTCGA
    ATGTCTTCTG AAGATAGTGA GCACTTGAAT GACAGGCAGG CTGACCAGCA AGAGCAAACA
    TCCAAGATTG ATGTGACTAG AGCGACAAAT GATGACTTTG GGAGGAGGAA ATTGTCTCTG
    CAAGAGAAAA GGGCTCGAAA CAAACAGAAG TTTGTACGCT TTGGCCCAGT GACTGAAATT
    GATCAGCAGA AGTGGGATAG GCTTTGTATA GCCAAGGCTG AATCTGGCCA GGAAAGTGAA
    GAGATGAAGG GCAGCATTAC GTCTGATGAA ACTGCCAGCC ACATATCTCC CTCAGCTTCT
    GCTCAGCGCT CCCAACCCCA GGAGGATGTT GGCACAGGGC CAGTCTTGCC ACATGAGTCA
    AGATCTTTTA CACCAGAGAG ATTGGTTTCA CCTGCTCCTC CTGAAAATGA AAGTGAAATT
    CAGCTCCTAG AAACACAGCA AGATGACATG ATGGCAAGGA GAACAGGTAC ATTCCAAAAA
    TGCGCCAGAA CCAGCAGAGA CTCTCCTGTG TCCCACCAAT ATGCACTCCA TAGCCAACAA
    CACGAAGAGA AGTCTGACTC GCTGGAACTC GAAGAGAAAC AAGGACTCTT TCTGGATGTT
    AACCCACAAC TGAGAAAATT ACTGCTGTCG GACTTGGCAA ATACTAGTTC AAAAGCAACT
    AGCCCTACTT TGGGGAGCAA GTCTCTGAAA GTCACCAAAG AAGCTGAAGA TGTAAAAAAT
    GACATGAAAG AAGGTGCCTT GAACACAGGA AAGGATCCAA ACTCTGTTAT CCCAGAGGAT
    AACACAATTG CACCAGACCC AGAAAAAGAT GACATGATGA CAAGAAAAAT CTCAGTGAGC
    CAGAAGCCAT CTTGCGTCAG CCTTGGACAC TTCCTTCCTG TTCCTTTTTC TTTGCAGCGC
    AGAGAGGGGG AAAAGAAACC AACAAGCTGC ATCATATCAA GCACCAAAAA GTCCAAAAAT
    GCATCCAAAG CTGTTCCAGC CGACATTTCT GATGTTCCTT CTAAGCCCCT TGACCAGAAA
    GAAACAAAAG AACATGGTGA ATTAGGAGAT ACGACTTGCA AGCAAGCCTC CAATAGTGTG
    GAGACTCCCA TGTGTACAGA GTTAGACAGC CAGCAACAGG ATGGTCTTGA GAGGAGAATG
    ATACTGTCTA CTGACTTGGC CAATACTGAG TTAAAAATAA CTCCTCCTAC TTTGGCCAAC
    AACTCTCCAA CCATAACAAA AGTACAAGAG CCAGATGCAA CCATCTCAGA GGACAATACA
    ACTGCACCAA ACCCTGAAAA AGATGATATG ATGACCAGAA GAATCTCTGT AACCCAGAAA
    CCATCGTGTG TAGGTCTTGG CCACTTCCTT CCAGTTCCAT TTTCTTTGCA GCAAAAAGTT
    GAGGAAAAGA AATCAACGAG CTCCACTCTA TCGATCAAAA TAAAGTCAGA TAATTGCACC
    CAAGCACTTG CAGTCGGCAG TTCTGACCTT CCTTGTAAGC TCCTCGGCCA ATGTGAAACA
    GAGGGACATG TTGAATTACG ATCCGATGTG ACTTGCGATC AAACCTCCAC TGAGATCATT
    AGTGATGGGA TTCCCACTGG TAAAGGGTTA GATACCCAGG CAGAAGACGC TCTTGAGACA
    TGTGACCATG AGTTATTCAG ATTCAGCCCA AGATCTGTTC TGTCTGATGA TGGAGAGAGC
    ATGAGCATGT GCGACATGAG GTACGAAGAA GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA
    CACTATGAGC ATCTACAGCA TGTCAACAGC TTGCTCAAGG AGGAGGATGA CACATGGCAA
    GATGACCTAG CTAAGTGGAA GACACGGAGA AGAAGTGCAT CACAGGATCT GATCAAAAAA
    GAGGAACAGA GGAAGAAGAT GGAGAGAATG ATGAGCGGAG AGGATGAACT GTCGGAGCGT
    AGGAAAAGCA TAAAAACATA TAGAGAGATT GTGGAAGAAA AAGAACGTCG AGAGAAAGAA
    CTGCATGAAG CCTACAAAAA TGCCAAGTCA AGAGATGAGG CTGAACACAT ATTACAGAGC
    TACATTGAAA AGTTTACAAT TAGTGAAGCT GTTCTTGAGG GATTAGTAAT GCCAAAAATC
    TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT TTATTGTCTT CATCAGAGGA CCCAAACATC
    CTGAAATACC TGAGGCAACA GTCTTTACCA TCACCAAAAT ACACTTCCAC TGTTGAAGCA
    ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA AAAAGTCCAA CAGGTCTGAT ATCACCAAGC
    AAAAACATTA TCTCAAAGAC TGTGCCTATG CTAACAGCCA AGCCTTACTC CCAGCCCAAA
    AACACCCAGG AAGTCCTCAA GACATTTAAG GTAGATGGAA AGGTAAACCT CAACGGGGTA
    GAAGAGCAGA AAGAAGAGGA ACATGGAAAC CAGACATTTG CCCCATCACT CACCCGATCT
    CAGATGTTTG AAGGTGTAGC CAGAGTTGAT GTTCCTGTTG TTGAAATAAA GCAAGACGTA
    ACTTCCATAG AGCTTAATCT GGCCAGACCA AACACCCTGA ATTCCTTACA AGAACCCAAA
    AGTCTCTGCG AAGACAGAAA TCACATAAGC CAGGAGGATG AGTCAGATTC TCTGGTGCAA
    TTAGAAAGAG CTCACTCCGT GGAAATATTG TCAGAAGGTA ACACCCAATC AAGCAATGCA
    ACCCAGGATG TACCTGTTGT GAGCAGTATA GAGATTCCAC CATTGACCAT CAATGGAAAG
    GACACTGTGG ATGAGAGGAA AGTGGAAGCT GCCGAGTTGA TCATGCATTG GTCCCTGGAG
    CCGAATCATA AGGAAGCCAA AGAAAATTCT TTATCGCCTT TAAAAACTTC TGAAAATTTA
    GAAGAATCCT TGCAGTTTAA AGAGAATGAA TCATCACACA AAGTGCAGCT AAACATTACG
    CTTCAGAGCC GGAACGCCTG GAGACGCTCC CAGTTTTTTT TGCAGCCAGT GGATACAGAA
    TGTACAGAAA ATTCACCAGC ACTTGTACCC AGTTTGAATT CTCCTAACCG ATTCTGCACA
    TGGGACCCTG AAGAAGAACG CAAACGGCAA GAAAAATGGC AGCAGGAGCA GGAAAGGCTT
    CTCCAGGAGA GGTACCAGAG AGAGCAGGAA AAACTTAAAG AAGAATGGGA AAAAGCTCAG
    AAAGAAGTAG AAGAGGAGGA AAGGAAATAT TATGAGGAGG AAAGAAAGAT AATTGAAGAC
    ACTGTGGTCC CACTGACTTT GTCTCCAAAT TCTAATGCAC AGTTTAATAT CTCTGAAAGA
    AATGGGAACT TCATTCCAGC ACTTACAGAA CATGTCAAGA GAGATCAAGA AAATAACAAT
    GAAAAGCTCT TGACAGAGTC CAGCAGAACG ATAAAGAATG AGACACATAT GCACAGCAAT
    GTAACACAGC AACTGCTTTT GCATACACAG GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA
    CAAATAAAGG GAAAAACAGA CAAGTGGGAA GCAGAACAGT CCATCTGTAT TATGGACCGA
    GAGGAGCTGA GCAACCATGT GCAGTCTAAT GAATGTAATA CACCCACTAG GTTACCCTTG
    CAGGCAGGAT GTAAACAAGT GACGTCATCA GAGTCAAGTC TGCCTGGGGA TCTTGGATCA
    CCAAAAAATC CTATCACTGA AAAAGATGCT ATTCAGAATA TGTCGTCCAT AATACCCACA
    CGGGGAACTG ATTTCAAGGA AGATACAATG AATTCTTCAC AGTCCTCTAC ATCACAGTGC
    CAGTCACCAA ACAGGTCTGT TAGTGGGAAA AAACTGTGCT CCACATGTGG ACTTCCTCTA
    GGCAAGGGAG CTGCCATGAT AATTGAAACC CTCAGTCTTT ATTTCCACAT CCAGTGCTTC
    AAGTGTGGAC TGTGTAAGGG ACAACTTGGG GATGCGATCA CCGGCACAGA CGTCAGAATT
    CGAAATGGCC TACTGAACTG CAATGACTGT TACATGAAGT CTAGAAGTAA GGCGGCTGGC
    CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751246.1
    CDS250..5547
    Translation

    Target ORF information:

    RefSeq Version XM_031895386.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGAAAAG AAAATGAGGA AATAGAGAGT CCAAAACGCA GCATACGGGA CAGTGGCTAC 
    ATAGATTGTT GGGACTCTGA GAGAAGTGAC TCCCTTTCTC CTCCCAGACA TGGTAGAGAT
    GACTCCTTCG ATAGTTTAGA CTCATTTGGA TCTAGATCAC AACAGACACC ATCTCCAGAT
    GTAGTGCTGA GGGGAAGCAG CGACGGCAGA GGAAGCGACT CTGAGTCTGA CCTACCTCAC
    AGAAGGTTAC CTGATGTGCG AAAAGATGAT ATGTCTGCTA GACGGGTATC CTACGGTGAA
    AACAAAGCAT TTGTGCCTTT CAACCAATAC CTCCCCAACA AGAGCAACCA GACCTCCTAC
    ATCCCGGCAC CCCTGAGGAA GAAGAAGGCT GAGCGTGAGG ACTACCGGAA AAGCTGGAGC
    ACAGCTACAT CCCCCCTGGG GGGAGAGCGA CCCTTCAGTC CCAAACAAAG TCCAGTCATT
    AAAGAAGAAC AAAACGAGTT GCAGTGTGAA GGAGAAGGGA CCAAGCAGAT GATTGCCTTA
    AAAGAACCTT GCCTTGGAGC AGAAAATAGG AGTGAGAGAG TTGGTGAAAG TGAAGGGGGT
    GTCATGGGAC AATCATCTGC TAATGATGAC ATTCCAAAAG ATGCAGAGGA AGTGAGAAAG
    CGCAAGCACC TTCAGAAAGC GGGTATCAAG GTCTTGCCCG CAACAAAGCG CTACAGCAGT
    CCAAGTCAAT TTGCTGAAGA CATTGATGGC GCTCCAGACA TTGTGCTGCG AAAAGAGAAC
    ACCTTCCTGA AGTCATATGA AGGGATGGGC TCTGATTCCG AAGAAGAAGA AGAGTGCAAA
    GTACCTGATA TACAGCGAGA TGACTTGGCC ACAAGGCGTG CAAGGATGAA CCAACCAAAA
    AGCCCAATGC AGTTTAATCA GTATATACAG GGGTCATACT TAAACAAAAA CAGAGCATCA
    AGAAAGCTGA ACAGTTCTAA GACTTCCACT CCAGCTTCTC CAAAAATGTC TCAAGATATC
    AGGGAGTACC AAGGCCCCCA GTTATTAAAG CCATCACACG TCTGCAAAGA AAAGACATTT
    CCAATACCCT CAGACAATAA AGAGGATGCA GAAGCAGAGC AAGAAAAAGT TCCCGACATT
    CAGAAAGACG ACCTGGCGAA ACGCAAAGCT AAAGCTTCAG TTTCACTGCC AAAGGAACCA
    TCGGCCTTTG CAGCGGTTTC AATTACAAAG GCCGACATGG AAACTTGGCA GAGGCTTCGA
    ATGTCTTCTG AAGATAGTGA GCACTTGAAT GACAGGCAGG CTGACCAGCA AGAGCAAACA
    TCCAAGATTG ATGTGACTAG AGCGACAAAT GATGACTTTG GGAGGAGGAA ATTGTCTCTG
    CAAGAGAAAA GGGCTCGAAA CAAACAGAAG TTTGTACGCT TTGGCCCAGT GACTGAAATT
    GATCAGCAGA AGTGGGATAG GCTTTGTATA GCCAAGGCTG AATCTGGCCA GGAAAGTGAA
    GAGATGAAGG GCAGCATTAC GTCTGATGAA ACTGCCAGCC ACATATCTCC CTCAGCTTCT
    GCTCAGCGCT CCCAACCCCA GGAGGATGTT GGCACAGGGC CAGTCTTGCC ACATGAGTCA
    AGATCTTTTA CACCAGAGAG ATTGGTTTCA CCTGCTCCTC CTGAAAATGA AAGTGAAATT
    CAGCTCCTAG AAACACAGCA AGATGACATG ATGGCAAGGA GAACAGGTAC ATTCCAAAAA
    TGCGCCAGAA CCAGCAGAGA CTCTCCTGTG TCCCACCAAT ATGCACTCCA TAGCCAACAA
    CACGAAGAGA AGTCTGACTC GCTGGAACTC GAAGAGAAAC AAGGACTCTT TCTGGATGTT
    AACCCACAAC TGAGAAAATT ACTGCTGTCG GACTTGGCAA ATACTAGTTC AAAAGCAACT
    AGCCCTACTT TGGGGAGCAA GTCTCTGAAA GTCACCAAAG AAGCTGAAGA TGTAAAAAAT
    GACATGAAAG AAGGTGCCTT GAACACAGGA AAGGATCCAA ACTCTGTTAT CCCAGAGGAT
    AACACAATTG CACCAGACCC AGAAAAAGAT GACATGATGA CAAGAAAAAT CTCAGTGAGC
    CAGAAGCCAT CTTGCGTCAG CCTTGGACAC TTCCTTCCTG TTCCTTTTTC TTTGCAGCGC
    AGAGAGGGGG AAAAGAAACC AACAAGCTGC ATCATATCAA GCACCAAAAA GTCCAAAAAT
    GCATCCAAAG CTGTTCCAGC CGACATTTCT GATGTTCCTT CTAAGCCCCT TGACCAGAAA
    GAAACAAAAG AACATGGTGA ATTAGGAGAT ACGACTTGCA AGCAAGCCTC CAATAGTGTG
    GAGACTCCCA TGTGTACAGA GTTAGACAGC CAGCAACAGG ATGGTCTTGA GAGGAGAATG
    ATACTGTCTA CTGACTTGGC CAATACTGAG TTAAAAATAA CTCCTCCTAC TTTGGCCAAC
    AACTCTCCAA CCATAACAAA AGTACAAGAG CCAGATGCAA CCATCTCAGA GGACAATACA
    ACTGCACCAA ACCCTGAAAA AGATGATATG ATGACCAGAA GAATCTCTGT AACCCAGAAA
    CCATCGTGTG TAGGTCTTGG CCACTTCCTT CCAGTTCCAT TTTCTTTGCA GCAAAAAGTT
    GAGGAAAAGA AATCAACGAG CTCCACTCTA TCGATCAAAA TAAAGTCAGA TAATTGCACC
    CAAGCACTTG CAGTCGGCAG TTCTGACCTT CCTTGTAAGC TCCTCGGCCA ATGTGAAACA
    GAGGGACATG TTGAATTACG ATCCGATGTG ACTTGCGATC AAACCTCCAC TGAGATCATT
    AGTGATGGGA TTCCCACTGG TAAAGGGTTA GATACCCAGG CAGAAGACGC TCTTGAGACA
    TGTGACCATG AGTTATTCAG ATTCAGCCCA AGATCTGTTC TGTCTGATGA TGGAGAGAGC
    ATGAGCATGT GCGACATGAG GTACGAAGAA GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA
    CACTATGAGC ATCTACAGCA TGTCAACAGC TTGCTCAAGG AGGAGGATGA CACATGGCAA
    GATGACCTAG CTAAGTGGAA GACACGGAGA AGAAGTGCAT CACAGGATCT GATCAAAAAA
    GAGGAACAGA GGAAGAAGAT GGAGAGAATG ATGAGCGGAG AGGATGAACT GTCGGAGCGT
    AGGAAAAGCA TAAAAACATA TAGAGAGATT GTGGAAGAAA AAGAACGTCG AGAGAAAGAA
    CTGCATGAAG CCTACAAAAA TGCCAAGTCA AGAGATGAGG CTGAACACAT ATTACAGAGC
    TACATTGAAA AGTTTACAAT TAGTGAAGCT GTTCTTGAGG GATTAGTAAT GCCAAAAATC
    TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT TTATTGTCTT CATCAGAGGA CCCAAACATC
    CTGAAATACC TGAGGCAACA GTCTTTACCA TCACCAAAAT ACACTTCCAC TGTTGAAGCA
    ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA AAAAGTCCAA CAGGTCTGAT ATCACCAAGC
    AAAAACATTA TCTCAAAGAC TGTGCCTATG CTAACAGCCA AGCCTTACTC CCAGCCCAAA
    AACACCCAGG AAGTCCTCAA GACATTTAAG GTAGATGGAA AGGTAAACCT CAACGGGGTA
    GAAGAGCAGA AAGAAGAGGA ACATGGAAAC CAGACATTTG CCCCATCACT CACCCGATCT
    CAGATGTTTG AAGGTGTAGC CAGAGTTGAT GTTCCTGTTG TTGAAATAAA GCAAGACGTA
    ACTTCCATAG AGCTTAATCT GGCCAGACCA AACACCCTGA ATTCCTTACA AGAACCCAAA
    AGTCTCTGCG AAGACAGAAA TCACATAAGC CAGGAGGATG AGTCAGATTC TCTGGTGCAA
    TTAGAAAGAG CTCACTCCGT GGAAATATTG TCAGAAGGTA ACACCCAATC AAGCAATGCA
    ACCCAGGATG TACCTGTTGT GAGCAGTATA GAGATTCCAC CATTGACCAT CAATGGAAAG
    GACACTGTGG ATGAGAGGAA AGTGGAAGCT GCCGAGTTGA TCATGCATTG GTCCCTGGAG
    CCGAATCATA AGGAAGCCAA AGAAAATTCT TTATCGCCTT TAAAAACTTC TGAAAATTTA
    GAAGAATCCT TGCAGTTTAA AGAGAATGAA TCATCACACA AAGTGCAGCT AAACATTACG
    CTTCAGAGCC GGAACGCCTG GAGACGCTCC CAGTTTTTTT TGCAGCCAGT GGATACAGAA
    TGTACAGAAA ATTCACCAGC ACTTGTACCC AGTTTGAATT CTCCTAACCG ATTCTGCACA
    TGGGACCCTG AAGAAGAACG CAAACGGCAA GAAAAATGGC AGCAGGAGCA GGAAAGGCTT
    CTCCAGGAGA GGTACCAGAG AGAGCAGGAA AAACTTAAAG AAGAATGGGA AAAAGCTCAG
    AAAGAAGTAG AAGAGGAGGA AAGGAAATAT TATGAGGAGG AAAGAAAGAT AATTGAAGAC
    ACTGTGGTCC CACTGACTTT GTCTCCAAAT TCTAATGCAC AGTTTAATAT CTCTGAAAGA
    AATGGGAACT TCATTCCAGC ACTTACAGAA CATGTCAAGA GAGATCAAGA AAATAACAAT
    GAAAAGCTCT TGACAGAGTC CAGCAGAACG ATAAAGAATG AGACACATAT GCACAGCAAT
    GTAACACAGC AACTGCTTTT GCATACACAG GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA
    CAAATAAAGG GAAAAACAGA CAAGTGGGAA GCAGAACAGT CCATCTGTAT TATGGACCGA
    GAGGAGCTGA GCAACCATGT GCAGTCTAAT GAATGTAATA CACCCACTAG GTTACCCTTG
    CAGGCAGGAT GTAAACAAGT GACGTCATCA GAGTCAAGTC TGCCTGGGGA TCTTGGATCA
    CCAAAAAATC CTATCACTGA AAAAGATGCT ATTCAGAATA TGTCGTCCAT AATACCCACA
    CGGGGAACTG ATTTCAAGGA AGATACAATG AATTCTTCAC AGTCCTCTAC ATCACAGTGC
    CAGTCACCAA ACAGGTCTGT TAGTGGGAAA AAACTGTGCT CCACATGTGG ACTTCCTCTA
    GGCAAGGGAG CTGCCATGAT AATTGAAACC CTCAGTCTTT ATTTCCACAT CCAGTGCTTC
    AAGTGTGGAC TGTGTAAGGG ACAACTTGGG GATGCGATCA CCGGCACAGA CGTCAGAATT
    CGAAATGGCC TACTGAACTG CAATGACTGT TACATGAAGT CTAGAAGTAA GGCGGCTGGC
    CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04746
    Clone ID Related Accession (Same CDS sequence) XM_004911217.3
    Accession Version XM_004911217.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911217.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911274.1
    CDS492..6266
    Translation

    Target ORF information:

    RefSeq Version XM_004911217.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911217.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC
    CCCACCCCTT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04747
    Clone ID Related Accession (Same CDS sequence) XM_004911218.3
    Accession Version XM_004911218.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC
    CCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911275.1
    CDS492..6260
    Translation

    Target ORF information:

    RefSeq Version XM_004911218.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911218.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC
    AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG
    TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA
    AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC
    CCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04749
    Clone ID Related Accession (Same CDS sequence) XM_004911220.3 , XM_004911221.3
    Accession Version XM_004911220.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911277.1
    CDS492..6095
    Translation

    Target ORF information:

    RefSeq Version XM_004911220.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911220.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04749
    Clone ID Related Accession (Same CDS sequence) XM_004911220.3 , XM_004911221.3
    Accession Version XM_004911221.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911278.1
    CDS492..6095
    Translation

    Target ORF information:

    RefSeq Version XM_004911221.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911221.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA
    GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT
    GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT
    GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA
    AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA
    ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA
    ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG
    GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG
    GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA
    AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG
    GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG
    TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG
    TCAGAATTCG AAATGGCCTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30867
    Clone ID Related Accession (Same CDS sequence) XM_012955695.2
    Accession Version XM_012955695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTGAAGA CGCCAAAAGC TCAAGTTACT GGCAAAAGCT TTGGCGACAA AGACTTCAGG 
    ACTGGACTGG AGAATGGAAT CCTTCTGTGC GAGTTATTAA ATGCTATTAA ACCAGGACTC
    GTAAAAAAGA TCAACAGACT TCCTACTCCC ATTGCTGGAT TGGACAATAT AACATTATTC
    CTTAGAGGCT GCAAAGAGCT CGGGCTGAAA GAATCTCAGT TGTTTGACCC AGGTGACCTA
    CAGGACACAG CAAACAGGAC AACAGCCAGG AATTCAGATT GCAACAGAAA ACTAAGAAAT
    GTACTAATCA CCGTGTACTG GCTGGGGAAA GCTGCAAGCA GCTGCTCGTC CTATAGTGGC
    AACAACCTGG ACTTAAAGGA GTTTGAGGGA TTGCTGGCAC AAATGAGAAA AGAAAATGAG
    GAAATAGAGA GTCCAAAACG CAGCATACGG GACAGTGGCT ACATAGATTG TTGGGACTCT
    GAGAGAAGTG ACTCCCTTTC TCCTCCCAGA CATGGTAGAG ATGACTCCTT CGATAGTTTA
    GACTCATTTG GATCTAGATC ACAACAGACA CCATCTCCAG ATGTAGTGCT GAGGGGAAGC
    AGCGACGGCA GAGGAAGCGA CTCTGAGTCT GACCTACCTC ACAGAAGGTT ACCTGATGTG
    CGAAAAGATG ATATGTCTGC TAGACGGGTA TCCTACGGTG AAAACAAAGC ATTTGTGCCT
    TTCAACCAAT ACCTCCCCAA CAAGAGCAAC CAGACCTCCT ACATCCCGGC ACCCCTGAGG
    AAGAAGAAGG CTGAGCGTGA GGACTACCGG AAAAGCTGGA GCACAGCTAC ATCCCCCCTG
    GGGGGAGAGC GACCCTTCAG TCCCAAACAA AGTCCAGTCA TTAAAGAAGA ACAAAACGAG
    TTGCAGTGTG AAGGAGAAGG GACCAAGCAG ATGATTGCCT TAAAAGAACC TTGCCTTGGA
    GCAGAAAATA GGAGTGAGAG AGTTGGTGAA AGTGAAGGGG GTGTCATGGG ACAATCATCT
    GCTAATGATG ACATTCCAAA AGATGCAGAG GAAGTGAGAA AGCGCAAGCA CCTTCAGAAA
    GCGGGTATCA AGGTCTTGCC CGCAACAAAG CGCTACAGCA GTCCAAGTCA ATTTGCTGAA
    GACATTGATG GCGCTCCAGA CATTGTGCTG CGAAAAGAGA ACACCTTCCT GAAGTCATAT
    GAAGGGATGG GCTCTGATTC CGAAGAAGAA GAAGAGTGCA AAGTACCTGA TATACAGCGA
    GATGACTTGG CCACAAGGCG TGCAAGGATG AACCAACCAA AAAGCCCAAT GCAGTTTAAT
    CAGTATATAC AGGGGTCATA CTTAAACAAA AACAGAGCAT CAAGAAAGCT GAACAGTTCT
    AAGACTTCCA CTCCAGCTTC TCCAAAAATG TCTCAAGATA TCAGGGAGTA CCAAGGCCCC
    CAGTTATTAA AGCCATCACA CGTCTGCAAA GAAAAGACAT TTCCAATACC CTCAGACAAT
    AAAGAGGATG CAGAAGCAGA GCAAGAAAAA GTTCCCGACA TTCAGAAAGA CGACCTGGCG
    AAACGCAAAG CTAAAGCTTC AGTTTCACTG CCAAAGGAAC CATCGGCCTT TGCAGCGGTT
    TCAATTACAA AGGCCGACAT GGAAACTTGG CAGAGGCTTC GAATGTCTTC TGAAGATAGT
    GAGCACTTGA ATGACAGGCA GGCTGACCAG CAAGAGCAAA CATCCAAGAT TGATGTGACT
    AGAGCGACAA ATGATGACTT TGGGAGGAGG AAATTGTCTC TGCAAGAGAA AAGGGCTCGA
    AACAAACAGA AGTTTGTACG CTTTGGCCCA GTGACTGAAA TTGATCAGCA GAAGTGGGAT
    AGGCTTTGTA TAGCCAAGGC TGAATCTGGC CAGGAAAGTG AAGAGATGAA GGGCAGCATT
    ACGTCTGATG AAACTGCCAG CCACATATCT CCCTCAGCTT CTGCTCAGCG CTCCCAACCC
    CAGGAGGATG TTGGCACAGG GCCAGTCTTG CCACATGAGT CAAGATCTTT TACACCAGAG
    AGATTGGTTT CACCTGCTCC TCCTGAAAAT GAAAGTGAAA TTCAGCTCCT AGAAACACAG
    CAAGATGACA TGATGGCAAG GAGAACAGGT ACATTCCAAA AATGCACCAG AACCAGCAGA
    GACTCTCCTG TGTCCCACCA ATATGCACTC CATAGCCAAC AACACGAAGA GAAGTCTGAC
    TCGCTGGAAC TCGAAGAGAA ACAAGGACTC TTTCTGGATG TTAACCCACA ACTGAGAAAA
    TTACTGCTGT CGGACTTGGC AAATACTAGT TCAAAAGCAA CTAGCCCTAC TTTGGGGAGC
    AAGTCTCTGA AAGTCACCAA AGAAGCTGAA GATGTAAAAA ATGACATGAA AGAAGGTGCC
    TTGAACACAG GAAAGGATCC AAACTCTGTT ATCCCAGAGG ATAACACAAT TGCACCAGAC
    CCAGAAAAAG ATGACATGAT GACAAGAAAA ATCTCAGTGA GCCAGAAGCC ATCTTGCGTC
    AGCCTTGGAC ACTTCCTTCC TGTTCCTTTT TCTTTGCAGC ACAGAGAGGG GGAAAAGAAA
    CCAACAAGCT GCATCATATC AAGCACCAAA AAGTCCAAAA ATGCATCCAA AGCTGTTCCA
    GCCGACATTT CTGATGTTCC TTCTAAGCCC CTTGACCAGA AAGAAACAAA AGAACATGGT
    GAATTAGGAG ATACGACTTG CAAGCAAGCC TCCAATAGTG TGGAGACTCC CATGTGTACA
    GAGTTAGACA GCCAGCAACA GGATGGTCTT GAGAGGAGAA TGATACTGTC TACTGACTTG
    GCCAATACTG AGTTAAAAAT AACTCCTCCT ACTTTGGCCA ACAACTCTCC AACCATAACA
    AAAGTACAAG AGCCAGATGC AACCATCTCA GAGGACAATA CAACTGCACC AAACCCTGAA
    AAAGATGATA TGATGACCAG AAGAATCTAT GTAACCCAGA AACCATCGTG TGTAGGTCTT
    GGCCACTTCC TTCCAGTTCC ATTTTCTTTG CAGCAAAAAG TTGAGGAAAA GAAATCAACG
    AGCTCCACTC TATCGATCAA AATAAAGTCA GATAATTGCA CCCAAGCACT TGCAGTCGGC
    AGTTCTGACC TTCCTTGTAA GCTCCTCGGC CAATGTGAAA CAGAGGGACA TGTTGAATTA
    CGATCCGATG TGACTTGCGA TCAAACCTCC ACTGAGATCA TTAGTGATGG GATTCCCACT
    GGTAAAGGGT TAGATACCCA GGCAGAAGAC GCTCTTGAGA CATGTGACCA TGAGTTATTC
    AGATTCAGCC CAAGATCTGT TCTGTCTGAT GATGGAGAGA GCATGAGCAT GTGCGACATG
    AGGTACGAAG AAGAGGGGAG TATGCAGCCT CACAGCAGGG CACACTATGA GCATCTACAG
    CATGTCAACA GCTTGCTCAA GGAGGAGGAT GACACATGGC AAGATGACCT AGCTAAGTGG
    AAGACACGGA GAAGAAGTGC ATCACAGGAT CTGATCAAAA AAGAGGAACA GAGGAAGAAG
    ATGGAGAGAA TGATGAGCGG AGAGGATGAA CTGTCGGAGC GTAGGAAAAG CATAAAAACA
    TATAGAGAGA TTGTGGAAGA AAAAGAACGT CGAGAGAAAG AACTGCATGA AGCCTACAAA
    AATGCCAAGT CAAGAGATGA GGCTGAACAC ATATTACAGA GCTACATTGA AAAGTTTACA
    ATTAGTGAAG CTGTTCTTGA GGGATTAGTA ATGCCAAAAA TCTTAGAAAG AAGCCAGTCG
    GTGGAGCCAA GTTTATTGTC TTCATCAGAG GACCCAAACA TCCTGAAATA CCTGAGGCAA
    CAGTCTTTAC CATCACCAAA ATACACTTCC ACTGTTGAAG CAACTGTCTC CCCCAGCAGC
    CCATGTGAAG CAAAAAGTCC AACAGGTCTG ATATCACCAA GCAAAAACAT TATCTCAAAG
    ACTGTGCCTA TGCTAACAGC CAAGCCTTAC TCCCAGCCCA AAAACACCCA GGAAGTCCTC
    AAGACATTTA AGGTAGATGG AAAGGTAAAC CTCAACGGGG TAGAAGAGCA GAAAGAAGAG
    GAACATGGAA ACCAGACATT TGCCCCATCA CTCACCCGAT CTCAGATGTT TGAAGGTGTA
    GCCAGAGTTG ATGTTCCTGT TGTTGAAATA AAGCAAGACG TAACTTCCAT AGAGCTTAAT
    CTGGCCAGAC CAAACACCCT GAATTCCTTA CAAGAACCCA AAAGTCTCTG CGAAGACAGA
    AATCACATAA GCCAGGAGGA TGAGTCAGAT TCTCTGGTGC AATTAGAAAG AGCTCACTCC
    GTGGAAATAT TGTCAGAAGG TAACACCCAA TCAAGCAATG CAACCCAGGA TGTACCTGTT
    GTGAGCAGTA TAGAGATTCC ACCATTGACC ATCAATGGAA AGGACACTGT GGATGAGAGG
    AAAGTGGAAG CTGCCGAGTT GATCATGCAT TGGTCCCTGG AGCCGAATCA TAAGGAAGCC
    AAAGAAAATT CTTTATCGCC TTTAAAAACT TCTGAAAATT TAGAAGAATC CTTGCAGTTT
    AAAGAGAATG AATCATCACA CAAAGTGCAG CTAAACATTA CGCTTCAGAG CCGGAACGCC
    TGGAGACGCT CCCAGTTTTT TTTGCAGCCA GTGGATACAG AATGTACAGA AAATTCACCA
    GCACTTGTAC CCAGTTTGAA TTCTCCTAAC CGATTCTGCA CATGGGACCC TGAAGAAGAA
    CGCAAACGGC AAGAAAAATG GCAGCAGGAG CAGGAAAGGC TTCTCCAGGA GAGGTACCAG
    AGAGAGCAGG AAAAACTTAA AGAAGAATGG GAAAAAGCTC AGAAAGAAGT AGAAGAGGAG
    GAAAGGAAAT ATTATGAGGA GGAAAGAAAG ATAATTGAAG ACACTGTGGT CCCACTGACT
    TTGTCTCCAA ATTCTAATGC ACAGTTTAAT ATCTCTGAAA GAAATGGGAA CTTCATTCCA
    GCACTTACAG AACATGTCAA GAGAGATCAA GAAAATAACA ATGAAAAGCT CTTGACAGAG
    TCCAGCAGAA CGATAAAGAA TGAGACACAT ATGCACAGCA ATGTAACACA GCAACTGCTT
    TTGCATACAC AGGAGGGGTC AATTCTTGGT GCTAAAGAGA AACAAATAAA GGGAAAAACA
    GACAAGTGGG AAGCAGAACA GTCCATCTGT ATTATGGACC GAGAGGAGCT GAGCAACCAT
    GTGCAGTCTA ATGAATGTAA TACACCCACT AGGTTACCCT TGCAGGCAGG ATGTAAACAA
    GTGACGTCAT CAGAGTCAAG TCTGCCTGGG GATCTTGGAT CACCAAAAAA TCCTATCACT
    GAAAAAGATG CTATTCAGAA TATGTCGTCC ATAATACCCA CACGGGGAAC TGATTTCAAG
    GAAGATACAA TGAATTCTTC ACAGTCCTCT ACATCACAGT GCCAGTCACC AAACAGGTCT
    GTTAGTGGGA AAAAACTGTG CTCCACATGT GGACTTCCTC TAGGCAAGGG AGCTGCCATG
    ATAATTGAAA CCCTCAGTCT TTATTTCCAC ATCCAGTGCT TCAAGTGTGG ACTGTGTAAG
    GGACAACTTG GGGATGCGAT CACCGGCACA GACGTCAGAA TTCGAAATGG CCTACTGAAC
    TGCAATGACT GTTACATGAA GTCTAGAAGT AAGGCGGCTG GCCACCCCAC CCCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811149.1
    CDS193..5892
    Translation

    Target ORF information:

    RefSeq Version XM_012955695.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTGAAGA CGCCAAAAGC TCAAGTTACT GGCAAAAGCT TTGGCGACAA AGACTTCAGG 
    ACTGGACTGG AGAATGGAAT CCTTCTGTGC GAGTTATTAA ATGCTATTAA ACCAGGACTC
    GTAAAAAAGA TCAACAGACT TCCTACTCCC ATTGCTGGAT TGGACAATAT AACATTATTC
    CTTAGAGGCT GCAAAGAGCT CGGGCTGAAA GAATCTCAGT TGTTTGACCC AGGTGACCTA
    CAGGACACAG CAAACAGGAC AACAGCCAGG AATTCAGATT GCAACAGAAA ACTAAGAAAT
    GTACTAATCA CCGTGTACTG GCTGGGGAAA GCTGCAAGCA GCTGCTCGTC CTATAGTGGC
    AACAACCTGG ACTTAAAGGA GTTTGAGGGA TTGCTGGCAC AAATGAGAAA AGAAAATGAG
    GAAATAGAGA GTCCAAAACG CAGCATACGG GACAGTGGCT ACATAGATTG TTGGGACTCT
    GAGAGAAGTG ACTCCCTTTC TCCTCCCAGA CATGGTAGAG ATGACTCCTT CGATAGTTTA
    GACTCATTTG GATCTAGATC ACAACAGACA CCATCTCCAG ATGTAGTGCT GAGGGGAAGC
    AGCGACGGCA GAGGAAGCGA CTCTGAGTCT GACCTACCTC ACAGAAGGTT ACCTGATGTG
    CGAAAAGATG ATATGTCTGC TAGACGGGTA TCCTACGGTG AAAACAAAGC ATTTGTGCCT
    TTCAACCAAT ACCTCCCCAA CAAGAGCAAC CAGACCTCCT ACATCCCGGC ACCCCTGAGG
    AAGAAGAAGG CTGAGCGTGA GGACTACCGG AAAAGCTGGA GCACAGCTAC ATCCCCCCTG
    GGGGGAGAGC GACCCTTCAG TCCCAAACAA AGTCCAGTCA TTAAAGAAGA ACAAAACGAG
    TTGCAGTGTG AAGGAGAAGG GACCAAGCAG ATGATTGCCT TAAAAGAACC TTGCCTTGGA
    GCAGAAAATA GGAGTGAGAG AGTTGGTGAA AGTGAAGGGG GTGTCATGGG ACAATCATCT
    GCTAATGATG ACATTCCAAA AGATGCAGAG GAAGTGAGAA AGCGCAAGCA CCTTCAGAAA
    GCGGGTATCA AGGTCTTGCC CGCAACAAAG CGCTACAGCA GTCCAAGTCA ATTTGCTGAA
    GACATTGATG GCGCTCCAGA CATTGTGCTG CGAAAAGAGA ACACCTTCCT GAAGTCATAT
    GAAGGGATGG GCTCTGATTC CGAAGAAGAA GAAGAGTGCA AAGTACCTGA TATACAGCGA
    GATGACTTGG CCACAAGGCG TGCAAGGATG AACCAACCAA AAAGCCCAAT GCAGTTTAAT
    CAGTATATAC AGGGGTCATA CTTAAACAAA AACAGAGCAT CAAGAAAGCT GAACAGTTCT
    AAGACTTCCA CTCCAGCTTC TCCAAAAATG TCTCAAGATA TCAGGGAGTA CCAAGGCCCC
    CAGTTATTAA AGCCATCACA CGTCTGCAAA GAAAAGACAT TTCCAATACC CTCAGACAAT
    AAAGAGGATG CAGAAGCAGA GCAAGAAAAA GTTCCCGACA TTCAGAAAGA CGACCTGGCG
    AAACGCAAAG CTAAAGCTTC AGTTTCACTG CCAAAGGAAC CATCGGCCTT TGCAGCGGTT
    TCAATTACAA AGGCCGACAT GGAAACTTGG CAGAGGCTTC GAATGTCTTC TGAAGATAGT
    GAGCACTTGA ATGACAGGCA GGCTGACCAG CAAGAGCAAA CATCCAAGAT TGATGTGACT
    AGAGCGACAA ATGATGACTT TGGGAGGAGG AAATTGTCTC TGCAAGAGAA AAGGGCTCGA
    AACAAACAGA AGTTTGTACG CTTTGGCCCA GTGACTGAAA TTGATCAGCA GAAGTGGGAT
    AGGCTTTGTA TAGCCAAGGC TGAATCTGGC CAGGAAAGTG AAGAGATGAA GGGCAGCATT
    ACGTCTGATG AAACTGCCAG CCACATATCT CCCTCAGCTT CTGCTCAGCG CTCCCAACCC
    CAGGAGGATG TTGGCACAGG GCCAGTCTTG CCACATGAGT CAAGATCTTT TACACCAGAG
    AGATTGGTTT CACCTGCTCC TCCTGAAAAT GAAAGTGAAA TTCAGCTCCT AGAAACACAG
    CAAGATGACA TGATGGCAAG GAGAACAGGT ACATTCCAAA AATGCACCAG AACCAGCAGA
    GACTCTCCTG TGTCCCACCA ATATGCACTC CATAGCCAAC AACACGAAGA GAAGTCTGAC
    TCGCTGGAAC TCGAAGAGAA ACAAGGACTC TTTCTGGATG TTAACCCACA ACTGAGAAAA
    TTACTGCTGT CGGACTTGGC AAATACTAGT TCAAAAGCAA CTAGCCCTAC TTTGGGGAGC
    AAGTCTCTGA AAGTCACCAA AGAAGCTGAA GATGTAAAAA ATGACATGAA AGAAGGTGCC
    TTGAACACAG GAAAGGATCC AAACTCTGTT ATCCCAGAGG ATAACACAAT TGCACCAGAC
    CCAGAAAAAG ATGACATGAT GACAAGAAAA ATCTCAGTGA GCCAGAAGCC ATCTTGCGTC
    AGCCTTGGAC ACTTCCTTCC TGTTCCTTTT TCTTTGCAGC ACAGAGAGGG GGAAAAGAAA
    CCAACAAGCT GCATCATATC AAGCACCAAA AAGTCCAAAA ATGCATCCAA AGCTGTTCCA
    GCCGACATTT CTGATGTTCC TTCTAAGCCC CTTGACCAGA AAGAAACAAA AGAACATGGT
    GAATTAGGAG ATACGACTTG CAAGCAAGCC TCCAATAGTG TGGAGACTCC CATGTGTACA
    GAGTTAGACA GCCAGCAACA GGATGGTCTT GAGAGGAGAA TGATACTGTC TACTGACTTG
    GCCAATACTG AGTTAAAAAT AACTCCTCCT ACTTTGGCCA ACAACTCTCC AACCATAACA
    AAAGTACAAG AGCCAGATGC AACCATCTCA GAGGACAATA CAACTGCACC AAACCCTGAA
    AAAGATGATA TGATGACCAG AAGAATCTAT GTAACCCAGA AACCATCGTG TGTAGGTCTT
    GGCCACTTCC TTCCAGTTCC ATTTTCTTTG CAGCAAAAAG TTGAGGAAAA GAAATCAACG
    AGCTCCACTC TATCGATCAA AATAAAGTCA GATAATTGCA CCCAAGCACT TGCAGTCGGC
    AGTTCTGACC TTCCTTGTAA GCTCCTCGGC CAATGTGAAA CAGAGGGACA TGTTGAATTA
    CGATCCGATG TGACTTGCGA TCAAACCTCC ACTGAGATCA TTAGTGATGG GATTCCCACT
    GGTAAAGGGT TAGATACCCA GGCAGAAGAC GCTCTTGAGA CATGTGACCA TGAGTTATTC
    AGATTCAGCC CAAGATCTGT TCTGTCTGAT GATGGAGAGA GCATGAGCAT GTGCGACATG
    AGGTACGAAG AAGAGGGGAG TATGCAGCCT CACAGCAGGG CACACTATGA GCATCTACAG
    CATGTCAACA GCTTGCTCAA GGAGGAGGAT GACACATGGC AAGATGACCT AGCTAAGTGG
    AAGACACGGA GAAGAAGTGC ATCACAGGAT CTGATCAAAA AAGAGGAACA GAGGAAGAAG
    ATGGAGAGAA TGATGAGCGG AGAGGATGAA CTGTCGGAGC GTAGGAAAAG CATAAAAACA
    TATAGAGAGA TTGTGGAAGA AAAAGAACGT CGAGAGAAAG AACTGCATGA AGCCTACAAA
    AATGCCAAGT CAAGAGATGA GGCTGAACAC ATATTACAGA GCTACATTGA AAAGTTTACA
    ATTAGTGAAG CTGTTCTTGA GGGATTAGTA ATGCCAAAAA TCTTAGAAAG AAGCCAGTCG
    GTGGAGCCAA GTTTATTGTC TTCATCAGAG GACCCAAACA TCCTGAAATA CCTGAGGCAA
    CAGTCTTTAC CATCACCAAA ATACACTTCC ACTGTTGAAG CAACTGTCTC CCCCAGCAGC
    CCATGTGAAG CAAAAAGTCC AACAGGTCTG ATATCACCAA GCAAAAACAT TATCTCAAAG
    ACTGTGCCTA TGCTAACAGC CAAGCCTTAC TCCCAGCCCA AAAACACCCA GGAAGTCCTC
    AAGACATTTA AGGTAGATGG AAAGGTAAAC CTCAACGGGG TAGAAGAGCA GAAAGAAGAG
    GAACATGGAA ACCAGACATT TGCCCCATCA CTCACCCGAT CTCAGATGTT TGAAGGTGTA
    GCCAGAGTTG ATGTTCCTGT TGTTGAAATA AAGCAAGACG TAACTTCCAT AGAGCTTAAT
    CTGGCCAGAC CAAACACCCT GAATTCCTTA CAAGAACCCA AAAGTCTCTG CGAAGACAGA
    AATCACATAA GCCAGGAGGA TGAGTCAGAT TCTCTGGTGC AATTAGAAAG AGCTCACTCC
    GTGGAAATAT TGTCAGAAGG TAACACCCAA TCAAGCAATG CAACCCAGGA TGTACCTGTT
    GTGAGCAGTA TAGAGATTCC ACCATTGACC ATCAATGGAA AGGACACTGT GGATGAGAGG
    AAAGTGGAAG CTGCCGAGTT GATCATGCAT TGGTCCCTGG AGCCGAATCA TAAGGAAGCC
    AAAGAAAATT CTTTATCGCC TTTAAAAACT TCTGAAAATT TAGAAGAATC CTTGCAGTTT
    AAAGAGAATG AATCATCACA CAAAGTGCAG CTAAACATTA CGCTTCAGAG CCGGAACGCC
    TGGAGACGCT CCCAGTTTTT TTTGCAGCCA GTGGATACAG AATGTACAGA AAATTCACCA
    GCACTTGTAC CCAGTTTGAA TTCTCCTAAC CGATTCTGCA CATGGGACCC TGAAGAAGAA
    CGCAAACGGC AAGAAAAATG GCAGCAGGAG CAGGAAAGGC TTCTCCAGGA GAGGTACCAG
    AGAGAGCAGG AAAAACTTAA AGAAGAATGG GAAAAAGCTC AGAAAGAAGT AGAAGAGGAG
    GAAAGGAAAT ATTATGAGGA GGAAAGAAAG ATAATTGAAG ACACTGTGGT CCCACTGACT
    TTGTCTCCAA ATTCTAATGC ACAGTTTAAT ATCTCTGAAA GAAATGGGAA CTTCATTCCA
    GCACTTACAG AACATGTCAA GAGAGATCAA GAAAATAACA ATGAAAAGCT CTTGACAGAG
    TCCAGCAGAA CGATAAAGAA TGAGACACAT ATGCACAGCA ATGTAACACA GCAACTGCTT
    TTGCATACAC AGGAGGGGTC AATTCTTGGT GCTAAAGAGA AACAAATAAA GGGAAAAACA
    GACAAGTGGG AAGCAGAACA GTCCATCTGT ATTATGGACC GAGAGGAGCT GAGCAACCAT
    GTGCAGTCTA ATGAATGTAA TACACCCACT AGGTTACCCT TGCAGGCAGG ATGTAAACAA
    GTGACGTCAT CAGAGTCAAG TCTGCCTGGG GATCTTGGAT CACCAAAAAA TCCTATCACT
    GAAAAAGATG CTATTCAGAA TATGTCGTCC ATAATACCCA CACGGGGAAC TGATTTCAAG
    GAAGATACAA TGAATTCTTC ACAGTCCTCT ACATCACAGT GCCAGTCACC AAACAGGTCT
    GTTAGTGGGA AAAAACTGTG CTCCACATGT GGACTTCCTC TAGGCAAGGG AGCTGCCATG
    ATAATTGAAA CCCTCAGTCT TTATTTCCAC ATCCAGTGCT TCAAGTGTGG ACTGTGTAAG
    GGACAACTTG GGGATGCGAT CACCGGCACA GACGTCAGAA TTCGAAATGG CCTACTGAAC
    TGCAATGACT GTTACATGAA GTCTAGAAGT AAGGCGGCTG GCCACCCCAC CCCTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30868
    Clone ID Related Accession (Same CDS sequence) XM_012955696.2
    Accession Version XM_012955696.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT
    GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA
    CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG
    CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG
    AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT
    CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT
    ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC
    AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT
    ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG
    TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG
    TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG
    TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA
    GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT
    ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT
    GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT
    GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA
    CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT
    GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811150.1
    CDS492..6185
    Translation

    Target ORF information:

    RefSeq Version XM_012955696.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955696.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT
    GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA
    CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG
    CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG
    AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT
    CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT
    ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC
    AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT
    ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG
    TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG
    TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG
    TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA
    GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT
    ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT
    GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT
    GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA
    CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT
    GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30869
    Clone ID Related Accession (Same CDS sequence) XM_012955698.2
    Accession Version XM_012955698.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT
    GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC
    AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG
    AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG
    TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA
    CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT
    AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA
    AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG
    AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT
    TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG
    TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT
    CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG
    ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG
    AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811152.1
    CDS492..6173
    Translation

    Target ORF information:

    RefSeq Version XM_012955698.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955698.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA
    CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC
    TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT
    GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA
    TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG
    GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA
    GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG
    GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT
    GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT
    GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG
    AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC
    TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT
    GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG
    GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG
    AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG
    CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC
    ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA
    GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG
    AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT
    GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA
    AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC
    ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA
    GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG
    ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT
    TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT
    CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA
    GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC
    CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC
    ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG
    AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA
    GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT
    CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT
    ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG
    GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC
    CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT
    GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC
    AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG
    AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG
    TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA
    CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT
    AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA
    AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG
    AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT
    TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG
    TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT
    CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG
    ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG
    AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30870
    Clone ID Related Accession (Same CDS sequence) XM_012955699.2
    Accession Version XM_012955699.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA
    TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT
    CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG
    ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA
    GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA
    AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA
    GAGGGGAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC
    TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA
    AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG
    ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT
    GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA
    AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT
    GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT
    TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA
    TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA
    AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG
    CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG
    GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC
    CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT
    GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA
    AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC
    CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG
    TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA
    GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT
    GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT
    TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA
    TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC
    CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC
    AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA
    GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA
    AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT
    TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT
    TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA
    CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG
    ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG
    GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA
    GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT
    GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA
    GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT
    ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG
    AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA
    AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC
    CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG
    GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT
    TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811153.1
    CDS492..5999
    Translation

    Target ORF information:

    RefSeq Version XM_012955699.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955699.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
    GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC
    GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT
    ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC
    AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT
    GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC
    AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC
    TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG
    AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA
    GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC
    TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA
    GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA
    AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC
    AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC
    CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA
    GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA
    GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA
    GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC
    ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC
    AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA
    AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC
    TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA
    CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC
    CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA
    AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG
    GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA
    ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG
    AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG
    GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG
    TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC
    AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA
    GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT
    CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG
    ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT
    CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA
    TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG
    CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC
    ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC
    GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC
    CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC
    CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC
    ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC
    ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG
    AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA
    GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA
    TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA
    ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG
    ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA
    TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT
    CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG
    ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA
    GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA
    AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA
    GAGGGGAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC
    TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA
    AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG
    ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT
    GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA
    AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT
    GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT
    TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA
    TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA
    AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG
    CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG
    GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC
    CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT
    GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA
    AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC
    CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG
    TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA
    GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT
    GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT
    TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA
    TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC
    CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC
    AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA
    GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA
    AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT
    TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT
    TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA
    CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG
    ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG
    GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA
    GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT
    GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA
    GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT
    ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG
    AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA
    AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC
    CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG
    GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT
    TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.