LIMCH1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015285466.1	XP_015140952.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_015285469.1	XP_015140955.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_015285468.2	XP_015140954.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_025149841.1	XP_025005609.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_015285463.1	XP_015140949.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_015285463.2	XP_015140949.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_015285465.1	XP_015140951.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_004936081.2	XP_004936138.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_025149839.1	XP_025005607.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_015285469.2	XP_015140955.1	LIM and calponin homology domains-containing protein 1 isoform X10
XM_015285462.2	XP_015140948.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_015285464.1	XP_015140950.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_015285462.1	XP_015140948.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_015285468.1	XP_015140954.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_015285466.2	XP_015140952.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_015285467.1	XP_015140953.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_025149840.1	XP_025005608.1	LIM and calponin homology domains-containing protein 1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa35487	XM_015285469.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa150224	XM_025149841.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa150224	XM_025149839.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa150224	XM_025149840.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa35480	XM_015285462.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35481	XM_015285463.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35482	XM_015285464.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35483	XM_015285465.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35484	XM_015285466.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35485	XM_015285467.1 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35486	XM_015285468.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa08412	XM_004936081.2 Latest version!	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OGa35481	XM_015285463.1	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35487	XM_015285469.1	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa35480	XM_015285462.1	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa35486	XM_015285468.1	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35484	XM_015285466.1	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

LIMCH1 ( XM_015285469.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140955.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa35487
Clone ID Related Accession (Same CDS sequence)	XM_015285469.1 , XM_015285469.2
Accession Version	XM_015285469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA 
CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC 
CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA 
AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT 
AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC 
TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT 
CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT 
AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT 
GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT 
GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG 
CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC 
GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG 
GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA 
ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA 
AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC 
ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG 
AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT 
GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT 
CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA 
CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT 
GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT 
CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA 
GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT 
GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC 
GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA 
CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT 
TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT 
GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA 
GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC 
CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC 
AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT 
AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG 
CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA 
GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA 
TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA 
AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC 
ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA 
AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG 
CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG 
AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG 
AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA 
CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG 
AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG 
TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC 
GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT 
GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG 
CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT 
GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140955.1
CDS	152..3979
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285469.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285469.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA 
CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC 
CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA 
AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT 
AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC 
TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT 
CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT 
AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT 
GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT 
GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG 
CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC 
GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG 
GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA 
ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA 
AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC 
ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG 
AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT 
GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT 
CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA 
CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT 
GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT 
CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA 
GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT 
GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC 
GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA 
CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT 
TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT 
GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA 
GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC 
CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC 
AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT 
AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG 
CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA 
GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA 
TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA 
AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC 
ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA 
AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG 
CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG 
AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG 
AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA 
CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG 
AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG 
TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC 
GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT 
GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG 
CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT 
GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285469.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140955.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa35487
Clone ID Related Accession (Same CDS sequence)	XM_015285469.1 , XM_015285469.2
Accession Version	XM_015285469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA 
CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC 
CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA 
AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT 
AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC 
TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT 
CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT 
AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT 
GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT 
GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG 
CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC 
GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG 
GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA 
ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA 
AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC 
ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG 
AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT 
GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT 
CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA 
CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT 
GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT 
CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA 
GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT 
GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC 
GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA 
CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT 
TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT 
GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA 
GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC 
CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC 
AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT 
AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG 
CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA 
GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA 
TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA 
AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC 
ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA 
AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG 
CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG 
AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG 
AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA 
CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG 
AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG 
TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC 
GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT 
GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG 
CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT 
GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140955.1
CDS	150..3977
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285469.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285469.2

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA 
CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC 
CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA 
AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT 
AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC 
TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT 
CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT 
AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT 
GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT 
GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG 
CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC 
GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG 
GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA 
ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA 
AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC 
ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG 
ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG 
AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT 
GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT 
CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA 
CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT 
GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT 
CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA 
GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT 
GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC 
GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA 
CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT 
TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT 
GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA 
GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC 
CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC 
AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT 
AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG 
CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA 
GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA 
TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA 
AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC 
ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA 
AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG 
CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG 
AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG 
AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA 
CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG 
AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG 
TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC 
GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT 
GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG 
CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT 
GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_025149841.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005609.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

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CloneID	OGa150224
Clone ID Related Accession (Same CDS sequence)	XM_025149841.1 , XM_025149839.1 , XM_025149840.1
Accession Version	XM_025149841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005609.1
CDS	406..5202
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_025149841.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149841.1

ORF Insert Sequence:

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_025149839.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005607.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

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CloneID	OGa150224
Clone ID Related Accession (Same CDS sequence)	XM_025149841.1 , XM_025149839.1 , XM_025149840.1
Accession Version	XM_025149839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005607.1
CDS	476..5272
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_025149839.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149839.1

ORF Insert Sequence:

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_025149840.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005608.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

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CloneID	OGa150224
Clone ID Related Accession (Same CDS sequence)	XM_025149841.1 , XM_025149839.1 , XM_025149840.1
Accession Version	XM_025149840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005608.1
CDS	431..5227
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_025149840.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149840.1

ORF Insert Sequence:

ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT 
GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC 
ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA 
CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA 
AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT 
CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA 
AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC 
AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA 
AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC 
AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC 
AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT 
AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC 
CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG 
ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT 
GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT 
GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA 
GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA 
GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT 
GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG 
TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG 
AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT 
CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA 
GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT 
GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA 
GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG 
GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA 
GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG 
AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG 
TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG 
CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG 
GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC 
ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA 
AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT 
CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA 
AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT 
GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC 
ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG 
AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT 
GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT 
GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG 
AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG 
GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG 
CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG 
AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT 
GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC 
CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT 
TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC 
AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA 
ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG 
CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC 
ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC 
CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA 
GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC 
TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG 
AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG 
AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA 
GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG 
GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT 
TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG 
CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG 
AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG 
GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT 
GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA 
ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA 
AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT 
GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG 
GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT 
GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG 
CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA 
CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT 
GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC 
AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG 
AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285462.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140948.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

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CloneID	OGa35480
Clone ID Related Accession (Same CDS sequence)	XM_015285462.2 , XM_015285462.1
Accession Version	XM_015285462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT 
GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG 
ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG 
GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC 
TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140948.1
CDS	158..5431
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285462.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285462.2

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT 
GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG 
ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG 
GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC 
TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285462.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140948.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa35480
Clone ID Related Accession (Same CDS sequence)	XM_015285462.2 , XM_015285462.1
Accession Version	XM_015285462.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT 
GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG 
ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG 
GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC 
TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140948.1
CDS	157..5430
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285462.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285462.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT 
GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG 
ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG 
GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC 
TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285463.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140949.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OGa35481
Clone ID Related Accession (Same CDS sequence)	XM_015285463.1 , XM_015285463.2
Accession Version	XM_015285463.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC 
AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT 
ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA 
CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC 
AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140949.1
CDS	158..5428
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285463.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285463.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC 
AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT 
ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA 
CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC 
AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285463.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140949.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OGa35481
Clone ID Related Accession (Same CDS sequence)	XM_015285463.1 , XM_015285463.2
Accession Version	XM_015285463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC 
AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT 
ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA 
CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC 
AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140949.1
CDS	159..5429
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285463.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285463.2

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC 
AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT 
ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA 
CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC 
AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285464.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140950.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

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CloneID	OGa35482
Clone ID Related Accession (Same CDS sequence)	XM_015285464.1
Accession Version	XM_015285464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTC TGTCAGCGGA 
AAGAAGCTTT GTTCCACCTG TGGGCTTCCT CTTGGCAAGG GAGCTGCCAT GATTATTGAA 
ACGCTTGGTC TTTATTTCCA TATTCAGTGT TTCAGGTGCG GCATTTGCAA GGGACAGCTG 
GGAGATGCAG CAAGTGGGAC AGATGTCAGG ATTAGAAATG GTCTTCTGAA CTGCAATGAT 
TGTTATATCC GATCCAGAAC TGCTGGACAG CCAACTACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140950.1
CDS	157..5421
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285464.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285464.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT 
ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC 
CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG 
TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG 
CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG 
CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTC TGTCAGCGGA 
AAGAAGCTTT GTTCCACCTG TGGGCTTCCT CTTGGCAAGG GAGCTGCCAT GATTATTGAA 
ACGCTTGGTC TTTATTTCCA TATTCAGTGT TTCAGGTGCG GCATTTGCAA GGGACAGCTG 
GGAGATGCAG CAAGTGGGAC AGATGTCAGG ATTAGAAATG GTCTTCTGAA CTGCAATGAT 
TGTTATATCC GATCCAGAAC TGCTGGACAG CCAACTACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285465.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140951.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

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CloneID	OGa35483
Clone ID Related Accession (Same CDS sequence)	XM_015285465.1
Accession Version	XM_015285465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTCTGT CAGCGGAAAG 
AAGCTTTGTT CCACCTGTGG GCTTCCTCTT GGCAAGGGAG CTGCCATGAT TATTGAAACG 
CTTGGTCTTT ATTTCCATAT TCAGTGTTTC AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA 
GATGCAGCAA GTGGGACAGA TGTCAGGATT AGAAATGGTC TTCTGAACTG CAATGATTGT 
TATATCCGAT CCAGAACTGC TGGACAGCCA ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140951.1
CDS	158..5419
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285465.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285465.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT 
CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT 
GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG 
AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG 
GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG 
CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT 
TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA 
GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG 
CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA 
CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC 
TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG 
TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC 
CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG 
CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA 
TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTCTGT CAGCGGAAAG 
AAGCTTTGTT CCACCTGTGG GCTTCCTCTT GGCAAGGGAG CTGCCATGAT TATTGAAACG 
CTTGGTCTTT ATTTCCATAT TCAGTGTTTC AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA 
GATGCAGCAA GTGGGACAGA TGTCAGGATT AGAAATGGTC TTCTGAACTG CAATGATTGT 
TATATCCGAT CCAGAACTGC TGGACAGCCA ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285466.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140952.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OGa35484
Clone ID Related Accession (Same CDS sequence)	XM_015285466.1 , XM_015285466.2
Accession Version	XM_015285466.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA 
GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG 
GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC 
CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA 
AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT 
ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT 
TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA 
CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC 
TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG 
GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT 
CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA 
GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT 
GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC 
ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA 
CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG 
AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA 
GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG 
GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG 
CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG 
GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT 
GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC 
AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT 
GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC 
AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140952.1
CDS	158..5209
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285466.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285466.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA 
GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG 
GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC 
CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA 
AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT 
ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT 
TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA 
CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC 
TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG 
GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT 
CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA 
GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT 
GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC 
ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA 
CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG 
AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA 
GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG 
GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG 
CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG 
GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT 
GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC 
AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT 
GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC 
AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285466.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140952.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OGa35484
Clone ID Related Accession (Same CDS sequence)	XM_015285466.1 , XM_015285466.2
Accession Version	XM_015285466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA 
GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG 
GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC 
CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA 
AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT 
ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT 
TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA 
CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC 
TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG 
GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT 
CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA 
GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT 
GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC 
ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA 
CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG 
AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA 
GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG 
GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG 
CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG 
GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT 
GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC 
AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT 
GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC 
AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140952.1
CDS	159..5210
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285466.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285466.2

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA 
GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG 
GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC 
CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA 
AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT 
ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT 
TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA 
CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC 
TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG 
GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT 
CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA 
GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT 
GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC 
ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA 
CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG 
AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA 
GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG 
GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG 
CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG 
GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT 
GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC 
AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT 
GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC 
AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285467.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140953.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

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CloneID	OGa35485
Clone ID Related Accession (Same CDS sequence)	XM_015285467.1
Accession Version	XM_015285467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG TGAAGAAGAT 
TGCAAGTGTC CCAGTGACAC TCTACCAGCA GCTGAAGTCA CAGTGCAAGC AGCAGTCCAG 
GATGATTTTG CTGTCCGCAA AGCCAGAGCA AACAGAAAGG CTGGTAGCCT CAAGCAGAAG 
TTTGTGCATT TTGGGCCAGT AACTGAGATT GATCAGCAGA AGTGGGACAA GCTCAGCATC 
GGCAGAGCTT CACGCAGGTG TGAAGCAGAA GAGGAAAGCG GGAAAGACGG AGTGAAGGTT 
CACTCGGATG TTGAAAGCTC TCTTCCAACC ACTGCTGGCA GTCTCCAGTT TGCTGAGCAT 
GGTGATGCAG TATCTAAGCC ACAGAATGAG CCCAGTGAGG TAGCGCAGTC AGATCCAGCT 
GAATGTTGCA GGAGCATGCC GTCACCTGCC GCTACTATTG ATGTCATCTC TGAGAAAGAA 
GCCCTGTGCA GCTCGAACAA TCAGCAAGAA AGCGCCGAAG AAGAAGAAAG CAGTGCAAAT 
TTACCCGACA TTGAGAGAGA CGATATGTTG GTTAGAAGGA TGGGGACCTT TCAGAGGCGC 
ACAACTAGCA CTGTTCCAAT CCACTGTCCT CCCCTGTCAG TAGCTCAGGA GAAGGATTTG 
AAGACTCCAC GGGGAGCAGA GAGTACTGTA ACGCGAGAAG AGAGCCAGGA ACAGCTTGAA 
ATCCCTCATC AGCAGAGTGG CGGAACAGAA AACAAAGTGA AGTTTCCAGA CCCACAAAGA 
GATGACATGT TGGCCAGAAG AACAGGAGCT TTTCTAAAAC AGCCGGGGCC AAGTGTTAAG 
CAGTTCCTTC CCATGCCATA TTCAAAGCAG CAGAATAAAC AGGGCACAAG TAGAGAACTT 
GAAAAGAAAA CTGCAAGGCC TGAAAAATGC CCACAAGTCT GCATTACTAG CTCTGGAAAA 
CCTGTAATTC TACCTCAGCA AGAGCAGCAA CAAGGGCAGG AAAGATGCAG TCTCCCTCCT 
GCAGGACAAG AGGATACTGT GTGCACTGCC CAAGCGGGTG ATCATAGCCT TAACGAAGTC 
CACCTTGATC AAACATCTAA GCTTCAGGTG GTTCCTGAGG AAAACAGAAC TGAAACATTG 
TCATCCAAGG ATGAGCATCT ACAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGCGTGA GCATGTTTGA CATGAGGAGT GAGGAAGAAG CCATGGCTCA GCCTCACAGC 
AAAGCTCGCC ATGAAAAGCT GCAGAATATA CACAACCAGC TGAAAGAGGA CGAGACCAGG 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GTGCTTCCCA GGATTTAATT 
AAAAAAGAAG CTGAGAGAAA GAAAATGGAA AGATTATTGT CTGGAGAGGG AACAAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAACGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG ACCCAGGAGG AGGCTGAGAG CATACTCCAG 
CAGTACATCG AAAGATTCAC CATTAGCGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATCCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC GAATCCTCTG 
AGATACTTAA GGCAACAATC ACTGCCTGCT CCAAAATTCA CTGCCACCAT TGAAGCAACC 
ATTGTTCCCA CCACTGAATT AGAGCCCAGT GGTTCGACAG GCCGCACATC TCCCAGCAAA 
AGTGTTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCTCA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAGAAT 
TTCAACGGAG TTGAAGAGAA GGATAAAGAG TGTACAACAG TAATATTTAC TCAATCGCCA 
AGCAGATCTC TGAAATTTGA TGGAGTAGCC AGAGGGGACG GGCCCCCCAT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAGA 
GAGCCAACAG CCTTGCCGGG GGAAGCAGAT CCGGCCGCCA GCCAGCAGGC CGAGGGCAGC 
CCTGGAGCAG TGGGACACGG GAGCCCGGCA GACCTGTCCC CAGGTTCAGA ACAAAAGCAA 
TTCAAGTCAA CTGTACTTTG TGCCAGCCCT GTTGTGAAGA GCTTAGAATT TTCTCCTAGT 
CCTGTCCCTC CAGAGGAAAC TGCTCCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
AAGCAGAAAG AGGCAGCACT CCCTGCTATG CAGGAGGTAA TGAAACCGAA AGCCCTAGAA 
CAAGAAGGGA GTGTAGTATT GCCATTTTTG AAGAAACTGC CAGAGACCAG CCAGGTCACT 
CTGCATAATT CTGATCTGCA AGAGCCACCC CGCAGGGTCA TGGTTCCGCT CAGGGTGCGG 
AACGACTGGC GGCGCTCCCG GTTTTTCTCA CAGCCAGCTG ATTCTGGCAA TAGTGACAAA 
TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG 
TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG 
AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG 
GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA 
GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT 
TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT 
TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG 
AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT 
GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA 
TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA 
GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA 
GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC 
TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG 
CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT 
GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT 
GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT 
ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140953.1
CDS	157..5175
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHASEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285467.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285467.1

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG TGAAGAAGAT 
TGCAAGTGTC CCAGTGACAC TCTACCAGCA GCTGAAGTCA CAGTGCAAGC AGCAGTCCAG 
GATGATTTTG CTGTCCGCAA AGCCAGAGCA AACAGAAAGG CTGGTAGCCT CAAGCAGAAG 
TTTGTGCATT TTGGGCCAGT AACTGAGATT GATCAGCAGA AGTGGGACAA GCTCAGCATC 
GGCAGAGCTT CACGCAGGTG TGAAGCAGAA GAGGAAAGCG GGAAAGACGG AGTGAAGGTT 
CACTCGGATG TTGAAAGCTC TCTTCCAACC ACTGCTGGCA GTCTCCAGTT TGCTGAGCAT 
GGTGATGCAG TATCTAAGCC ACAGAATGAG CCCAGTGAGG TAGCGCAGTC AGATCCAGCT 
GAATGTTGCA GGAGCATGCC GTCACCTGCC GCTACTATTG ATGTCATCTC TGAGAAAGAA 
GCCCTGTGCA GCTCGAACAA TCAGCAAGAA AGCGCCGAAG AAGAAGAAAG CAGTGCAAAT 
TTACCCGACA TTGAGAGAGA CGATATGTTG GTTAGAAGGA TGGGGACCTT TCAGAGGCGC 
ACAACTAGCA CTGTTCCAAT CCACTGTCCT CCCCTGTCAG TAGCTCAGGA GAAGGATTTG 
AAGACTCCAC GGGGAGCAGA GAGTACTGTA ACGCGAGAAG AGAGCCAGGA ACAGCTTGAA 
ATCCCTCATC AGCAGAGTGG CGGAACAGAA AACAAAGTGA AGTTTCCAGA CCCACAAAGA 
GATGACATGT TGGCCAGAAG AACAGGAGCT TTTCTAAAAC AGCCGGGGCC AAGTGTTAAG 
CAGTTCCTTC CCATGCCATA TTCAAAGCAG CAGAATAAAC AGGGCACAAG TAGAGAACTT 
GAAAAGAAAA CTGCAAGGCC TGAAAAATGC CCACAAGTCT GCATTACTAG CTCTGGAAAA 
CCTGTAATTC TACCTCAGCA AGAGCAGCAA CAAGGGCAGG AAAGATGCAG TCTCCCTCCT 
GCAGGACAAG AGGATACTGT GTGCACTGCC CAAGCGGGTG ATCATAGCCT TAACGAAGTC 
CACCTTGATC AAACATCTAA GCTTCAGGTG GTTCCTGAGG AAAACAGAAC TGAAACATTG 
TCATCCAAGG ATGAGCATCT ACAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGCGTGA GCATGTTTGA CATGAGGAGT GAGGAAGAAG CCATGGCTCA GCCTCACAGC 
AAAGCTCGCC ATGAAAAGCT GCAGAATATA CACAACCAGC TGAAAGAGGA CGAGACCAGG 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GTGCTTCCCA GGATTTAATT 
AAAAAAGAAG CTGAGAGAAA GAAAATGGAA AGATTATTGT CTGGAGAGGG AACAAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAACGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG ACCCAGGAGG AGGCTGAGAG CATACTCCAG 
CAGTACATCG AAAGATTCAC CATTAGCGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATCCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC GAATCCTCTG 
AGATACTTAA GGCAACAATC ACTGCCTGCT CCAAAATTCA CTGCCACCAT TGAAGCAACC 
ATTGTTCCCA CCACTGAATT AGAGCCCAGT GGTTCGACAG GCCGCACATC TCCCAGCAAA 
AGTGTTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCTCA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAGAAT 
TTCAACGGAG TTGAAGAGAA GGATAAAGAG TGTACAACAG TAATATTTAC TCAATCGCCA 
AGCAGATCTC TGAAATTTGA TGGAGTAGCC AGAGGGGACG GGCCCCCCAT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAGA 
GAGCCAACAG CCTTGCCGGG GGAAGCAGAT CCGGCCGCCA GCCAGCAGGC CGAGGGCAGC 
CCTGGAGCAG TGGGACACGG GAGCCCGGCA GACCTGTCCC CAGGTTCAGA ACAAAAGCAA 
TTCAAGTCAA CTGTACTTTG TGCCAGCCCT GTTGTGAAGA GCTTAGAATT TTCTCCTAGT 
CCTGTCCCTC CAGAGGAAAC TGCTCCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
AAGCAGAAAG AGGCAGCACT CCCTGCTATG CAGGAGGTAA TGAAACCGAA AGCCCTAGAA 
CAAGAAGGGA GTGTAGTATT GCCATTTTTG AAGAAACTGC CAGAGACCAG CCAGGTCACT 
CTGCATAATT CTGATCTGCA AGAGCCACCC CGCAGGGTCA TGGTTCCGCT CAGGGTGCGG 
AACGACTGGC GGCGCTCCCG GTTTTTCTCA CAGCCAGCTG ATTCTGGCAA TAGTGACAAA 
TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG 
TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG 
AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG 
GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA 
GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT 
TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT 
TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG 
AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT 
GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA 
TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA 
GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA 
GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC 
TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG 
CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT 
GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT 
GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT 
ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285468.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140954.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa35486
Clone ID Related Accession (Same CDS sequence)	XM_015285468.2 , XM_015285468.1
Accession Version	XM_015285468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC 
CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG 
CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC 
TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG 
AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC 
AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA 
GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA 
CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG 
GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA 
GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA 
AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG 
TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA 
GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT 
TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG 
AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC 
TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG 
CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC 
AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA 
GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG 
CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG 
AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT 
CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA 
GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA 
ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT 
GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT 
CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA 
CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG 
TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT 
CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC 
GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC 
CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG 
AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC 
GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA 
ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT 
TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT 
GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA 
GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG 
TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG 
CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA 
CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC 
ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG 
CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG 
TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG 
AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC 
TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG 
AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC 
ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA 
GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA 
CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA 
GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG 
CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC 
TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG 
GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT 
GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG 
CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG 
GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG 
AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT 
GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT 
GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC 
CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG 
CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA 
GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG 
TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG 
TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC 
CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA 
CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG 
GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC 
TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA 
GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA 
TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT 
CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG 
GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG 
AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG 
CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC 
TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG 
TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT 
CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG 
TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC 
AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA 
CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140954.1
CDS	108..4925
Translation	MDYERQVTFQETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285468.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285468.2

ORF Insert Sequence:

ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC 
CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG 
CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC 
TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG 
AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC 
AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA 
GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA 
CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG 
GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA 
GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA 
AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG 
TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA 
GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT 
TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG 
AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC 
TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG 
CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC 
AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA 
GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG 
CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG 
AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT 
CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA 
GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA 
ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT 
GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT 
CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA 
CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG 
TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT 
CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC 
GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC 
CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG 
AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC 
GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA 
ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT 
TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT 
GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA 
GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG 
TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG 
CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA 
CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC 
ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG 
CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG 
TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG 
AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC 
TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG 
AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC 
ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA 
GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA 
CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA 
GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG 
CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC 
TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG 
GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT 
GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG 
CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG 
GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG 
AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT 
GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT 
GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC 
CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG 
CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA 
GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG 
TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG 
TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC 
CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA 
CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG 
GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC 
TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA 
GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA 
TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT 
CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG 
GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG 
AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG 
CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC 
TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG 
TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT 
CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG 
TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC 
AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA 
CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_015285468.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140954.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

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CloneID	OGa35486
Clone ID Related Accession (Same CDS sequence)	XM_015285468.2 , XM_015285468.1
Accession Version	XM_015285468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC 
CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG 
CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC 
TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG 
AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC 
AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA 
GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA 
CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG 
GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA 
GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA 
AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG 
TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA 
GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT 
TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG 
AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC 
TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG 
CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC 
AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA 
GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG 
CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG 
AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT 
CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA 
GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA 
ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT 
GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT 
CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA 
CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG 
TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT 
CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC 
GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC 
CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG 
AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC 
GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA 
ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT 
TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT 
GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA 
GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG 
TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG 
CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA 
CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC 
ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG 
CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG 
TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG 
AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC 
TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG 
AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC 
ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA 
GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA 
CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA 
GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG 
CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC 
TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG 
GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT 
GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG 
CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG 
GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG 
AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT 
GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT 
GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC 
CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG 
CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA 
GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG 
TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG 
TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC 
CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA 
CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG 
GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC 
TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA 
GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA 
TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT 
CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG 
GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG 
AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG 
CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC 
TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG 
TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT 
CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG 
TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC 
AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA 
CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140954.1
CDS	124..4941
Translation	MDYERQVTFQETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQEPPRRVMVPLRVRNDWRRSRFFSQPADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_015285468.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285468.1

ORF Insert Sequence:

ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC 
CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG 
CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC 
TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG 
AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC 
AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA 
GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA 
CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG 
GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA 
GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA 
AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG 
TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA 
GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT 
TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG 
AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC 
TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG 
CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC 
AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA 
GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG 
CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG 
AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT 
CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA 
GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA 
ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT 
GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT 
CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA 
CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG 
TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT 
CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC 
GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC 
CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG 
AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC 
GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA 
ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT 
TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT 
GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA 
GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG 
TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG 
CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA 
CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC 
ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG 
CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG 
TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG 
AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC 
TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG 
AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC 
ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA 
GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA 
CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA 
GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG 
CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC 
TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG 
GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT 
GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG 
CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG 
GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG 
AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT 
GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT 
GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC 
CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG 
CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA 
GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG 
TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG 
TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC 
CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA 
CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG 
GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC 
TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA 
GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA 
TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT 
CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG 
GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG 
AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG 
CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC 
TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG 
TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT 
CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG 
TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC 
AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA 
CAGCCAACTA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_004936081.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004936138.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OGa08412
Clone ID Related Accession (Same CDS sequence)	XM_004936081.2
Accession Version	XM_004936081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004936081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGCTG ATTCTGGCAA TAGTGACAAA 
TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG 
TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG 
AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG 
GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA 
GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT 
TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT 
TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG 
AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT 
GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA 
TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA 
GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA 
GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC 
TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG 
CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT 
GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT 
GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT 
ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004936138.1
CDS	156..5354
Translation	MASPALELGEAQPPPDSPQPEPAFGEAQKWIEQVTGRSFGEKDFRSGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPGDLQDTSNRVTVKNVDCSRKLKNVLVTIYWLGKAANSCTSYSGSTLNLKEFEGLLAQMRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPETIEEEQSETSGLCEKEKMGSMAPEESPVKIQADLNQNEGDSIDQSPSCSVKETSSLKGKDEAEIRKLRRLEQAGIKVMSAAQRYGSKKQTCEDRGSTPDIILRKENPFMTRYQGKDSGSEDEAASKVPDLEKDDFATRRARMNQPKHVVPFNSFIIGPYTQKDKSKLEDNKKPLKVEKQEHARGSQVHMASARQTVTCKENPLLQTEKNKSEEEEQVKVVDAERDDLAKRKSQCVLPQLQEDRIFVNASITKADLETWKRLKLSGEPSEEDCKCPSDTLPAAEVTVQAAVQDDFAVRKARANRKAGSLKQKFVHFGPVTEIDQQKWDKLSIGRASRRCEAEEESGKDGVKVHSDVESSLPTTAGSLQFAEHGDAVSKPQNEPSEVAQSDPAECCRSMPSPAATIDVISEKEALCSSNNQQESAEEEESSANLPDIERDDMLVRRMGTFQRRTTSTVPIHCPPLSVAQEKDLKTPRGAESTVTREESQEQLEIPHQQSGGTENKVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPYSKQQNKQGTSRELEKKTARPEKCPQVCITSSGKPVILPQQEQQQGQERCSLPPAGQEDTVCTAQAGDHSLNEVHLDQTSKLQVVPEENRTETLSSKDEHLQTFGPRTAVSDDAESVSMFDMRSEEEAMAQPHSKARHEKLQNIHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGEGTNERRKSIKTYREIVEEKERRERELHEAYKNAKTQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATIEATIVPTTELEPSGSTGRTSPSKSVVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVIFTQSPSRSLKFDGVARGDGPPIELKKDPTSVELSLQRPATQSVHREPTALPGEADPAASQQAEGSPGAVGHGSPADLSPGSEQKQFKSTVLCASPVVKSLEFSPSPVPPEETAPSEKDVKKPEMEKQKEAALPAMQEVMKPKALEQEGSVVLPFLKKLPETSQVTLHNSDLQADSGNSDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTSDSNGSPEEGKPKEVTRQKKLVWEQDSIASLKNKENERWQEKRSSGNKVLSGHPEVGILKGGDQEYQVPVMERCSPARLNLPLTQDVPWNQQLLTKQSPHHAEDARQRPLLGQSAGQQPSAAGEGRRAGCHENFRSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCNDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_004936081.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004936081.2

ORF Insert Sequence:

ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT 
GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA 
ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT 
AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC 
GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT 
AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC 
ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG 
AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA 
GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT 
TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA 
GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA 
AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT 
AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA 
CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT 
GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA 
AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC 
CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT 
TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG 
TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT 
AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC 
CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA 
GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA 
CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA 
CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG 
GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT 
GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT 
TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG 
AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG 
CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG 
CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT 
AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG 
GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA 
GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC 
CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG 
CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC 
ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA 
GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG 
ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT 
CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC 
CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT 
CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG 
GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC 
ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT 
ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA 
TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT 
AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC 
AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT 
GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG 
GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA 
GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT 
TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA 
GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA 
GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT 
GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT 
TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA 
GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC 
ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC 
ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC 
TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC 
ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA 
TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC 
CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT 
CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG 
CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT 
TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA 
GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG 
AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA 
CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG 
ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGCTG ATTCTGGCAA TAGTGACAAA 
TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG 
TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG 
AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG 
GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA 
GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT 
TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT 
TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG 
AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT 
GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA 
TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA 
GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA 
GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC 
TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG 
CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT 
GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT 
GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT 
ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LIMCH1
Entrez Gene ID	422780
Full Name	LIM and calponin homology domains 1
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1
Homo sapiens (human)	LIMCH1	NP_055803.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LIMCH1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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