LIMCH1 cDNA ORF clone, Gallus gallus(chicken)

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa35487 XM_015285469.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa150224 XM_025149841.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa150224 XM_025149839.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa150224 XM_025149840.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa35480 XM_015285462.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35481 XM_015285463.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35482 XM_015285464.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35483 XM_015285465.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35484 XM_015285466.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35485 XM_015285467.1
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35486 XM_015285468.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa08412 XM_004936081.2
Latest version!
Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OGa35481 XM_015285463.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35487 XM_015285469.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa35480 XM_015285462.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35486 XM_015285468.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35484 XM_015285466.1 Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa35487
    Clone ID Related Accession (Same CDS sequence) XM_015285469.1 , XM_015285469.2
    Accession Version XM_015285469.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA
    CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC
    CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA
    AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT
    AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC
    TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT
    CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT
    AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT
    GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT
    GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG
    CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC
    GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG
    GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA
    ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA
    AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC
    ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG
    ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG
    AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT
    GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT
    CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA
    CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT
    GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT
    CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA
    GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT
    GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC
    GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA
    CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT
    TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT
    GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA
    GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC
    CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC
    AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT
    AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG
    CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA
    GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA
    TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA
    AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC
    ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA
    AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG
    CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG
    AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG
    AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA
    CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG
    AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG
    TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC
    GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT
    GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG
    CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT
    GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140955.1
    CDS152..3979
    Translation

    Target ORF information:

    RefSeq Version XM_015285469.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA
    CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC
    CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA
    AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT
    AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC
    TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT
    CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT
    AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT
    GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT
    GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG
    CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC
    GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG
    GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA
    ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA
    AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC
    ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG
    ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG
    AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT
    GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT
    CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA
    CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT
    GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT
    CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA
    GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT
    GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC
    GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA
    CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT
    TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT
    GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA
    GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC
    CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC
    AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT
    AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG
    CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA
    GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA
    TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA
    AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC
    ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA
    AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG
    CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG
    AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG
    AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA
    CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG
    AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG
    TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC
    GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT
    GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG
    CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT
    GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35487
    Clone ID Related Accession (Same CDS sequence) XM_015285469.1 , XM_015285469.2
    Accession Version XM_015285469.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA
    CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC
    CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA
    AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT
    AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC
    TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT
    CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT
    AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT
    GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT
    GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG
    CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC
    GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG
    GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA
    ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA
    AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC
    ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG
    ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG
    AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT
    GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT
    CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA
    CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT
    GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT
    CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA
    GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT
    GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC
    GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA
    CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT
    TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT
    GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA
    GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC
    CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC
    AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT
    AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG
    CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA
    GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA
    TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA
    AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC
    ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA
    AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG
    CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG
    AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG
    AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA
    CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG
    AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG
    TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC
    GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT
    GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG
    CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT
    GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140955.1
    CDS150..3977
    Translation

    Target ORF information:

    RefSeq Version XM_015285469.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285469.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGTACTGTAA CGCGAGAAGA GAGCCAGGAA
    CAGCTTGAAA TCCCTCATCA GCAGAGTGGC GGAACAGAAA ACAAAGTGAA GTTTCCAGAC
    CCACAAAGAG ATGACATGTT GGCCAGAAGA ACAGGAGCTT TTCTAAAACA GCCGGGGCCA
    AGTGTTAAGC AGTTCCTTCC CATGCCATAT TCAAAGCAGC AGAATAAACA GGGCACAAGT
    AGAGAACTTG AAAAGAAAAC TGCAAGGCCT GAAAAATGCC CACAAGTCTG CATTACTAGC
    TCTGGAAAAC CTGTAATTCT ACCTCAGCAA GAGCAGCAAC AAGGGCAGGA AAGATGCAGT
    CTCCCTCCTG CAGGACAAGA GGATACTGTG TGCACTGCCC AAGCGGGTGA TCATAGCCTT
    AACGAAGTCC ACCTTGATCA AACATCTAAG CTTCAGGTGG TTCCTGAGGA AAACAGAACT
    GAAACATTGT CATCCAAGGA TGAGCATCTA CAAACATTCG GCCCCAGAAC TGCTGTGTCT
    GATGACGCAG AGAGCGTGAG CATGTTTGAC ATGAGGAGTG AGGAAGAAGC CATGGCTCAG
    CCTCACAGCA AAGCTCGCCA TGAAAAGCTG CAGAATATAC ACAACCAGCT GAAAGAGGAC
    GAGACCAGGT GGCAAGATGA CCTGGCTCGG TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG
    GATTTAATTA AAAAAGAAGC TGAGAGAAAG AAAATGGAAA GATTATTGTC TGGAGAGGGA
    ACAAATGAGC GCAGAAAAAG CATCAAAACC TACCGAGAAA TTGTGGAAGA GAAAGAGAGA
    AGAGAACGGG AGCTTCATGA GGCATACAAG AATGCAAAGA CCCAGGAGGA GGCTGAGAGC
    ATACTCCAGC AGTACATCGA AAGATTCACC ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG
    ATGCCAAAAA TCCTGGAAAG AAGCCATTCA GTGGAGCCAA ATTCCCCACT GAAAGACCCG
    AATCCTCTGA GATACTTAAG GCAACAATCA CTGCCTGCTC CAAAATTCAC TGCCACCATT
    GAAGCAACCA TTGTTCCCAC CACTGAATTA GAGCCCAGTG GTTCGACAGG CCGCACATCT
    CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG CCTATGCTGA CACCCAAGCC TTACTCTCAA
    CCCAAAAATA CACAGGAAGT TTTAAAGAAC TTTAAGGTAG ATGGAAAAGT CAGCATGAAT
    GGAGAGAATT TCAACGGAGT TGAAGAGAAG GATAAAGAGT GTACAACAGT AATATTTACT
    CAATCGCCAA GCAGATCTCT GAAATTTGAT GGAGTAGCCA GAGGGGACGG GCCCCCCATA
    GAGTTGAAGA AGGACCCCAC AAGTGTTGAG CTCAGTTTAC AGAGGCCTGC CACTCAGAGT
    GTGCACAGAG AGCCAACAGC CTTGCCGGGG GAAGCAGATC CGGCCGCCAG CCAGCAGGCC
    GAGGGCAGCC CTGGAGCAGT GGGACACGGG AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA
    CAAAAGCAAT TCAAGTCAAC TGTACTTTGT GCCAGCCCTG TTGTGAAGAG CTTAGAATTT
    TCTCCTAGTC CTGTCCCTCC AGAGGAAACT GCTCCAAGTG AGAAAGATGT GAAGAAACCT
    GAAATGGAGA AGCAGAAAGA GGCAGCACTC CCTGCTATGC AGGAGGTAAT GAAACCGAAA
    GCCCTAGAAC AAGAAGGGAG TGTAGTATTG CCATTTTTGA AGAAACTGCC AGAGACCAGC
    CAGGTCACTC TGCATAATTC TGATCTGCAA GAGCCACCCC GCAGGGTCAT GGTTCCGCTC
    AGGGTGCGGA ACGACTGGCG GCGCTCCCGG TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT
    AGTGACAAAT CAAATCGTTT CAATTTCTGG TCCTGGGACC CAGAGGAAGA GCGGAAGCGG
    CAGGAGAGGT GGCAGCATGA GCAAGAACGC CTGCTTCAGG AGAAGTATCA GAAGGAGCAA
    GACAAACTGA AGGAGGAATG GGAAAAGGCA CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA
    TACTATGAGG AGGAACGTAA GATAATCGAG GACACTGTAG TTCCATTCAT TGTTTCTTCA
    AACTCTGCAG AACTCCTCTC CTCTTCTTCC TCTACCACTG AAGGAAATAA AACAGCGAAC
    ACAAGTGATT CAAATGGCTC TCCAGAGGAA GGCAAACCAA AAGAAGTTAC AAGGCAGAAA
    AAGCTGGTTT GGGAGCAGGA CAGCATAGCA TCTCTGAAAA ACAAGGAAAA TGAACGCTGG
    CAAGAAAAGA GGAGCAGTGG AAATAAAGTT CTCTCAGGAC ACCCAGAGGT GGGTATCTTG
    AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG GTGATGGAGC GCTGCTCGCC TGCCCGGTTG
    AATCTGCCAT TGACTCAGGA TGTCCCATGG AACCAGCAGC TGCTAACGAA GCAGTCCCCA
    CACCACGCAG AGGACGCCCG GCAGAGACCG CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG
    AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC TGTCATGAGA ACTTTCGATC TGGGCCATCG
    TCTCCCTGCT CACCTGCTGC ACCAAGTCAG TCACCCAACA GGTACAAAAG GTCTGTCAGC
    GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT CCTCTTGGCA AGGGAGCTGC CATGATTATT
    GAAACGCTTG GTCTTTATTT CCATATTCAG TGTTTCAGGT GCGGCATTTG CAAGGGACAG
    CTGGGAGATG CAGCAAGTGG GACAGATGTC AGGATTAGAA ATGGTCTTCT GAACTGCAAT
    GATTGTTATA TCCGATCCAG AACTGCTGGA CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150224
    Clone ID Related Accession (Same CDS sequence) XM_025149841.1 , XM_025149839.1 , XM_025149840.1
    Accession Version XM_025149841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005609.1
    CDS406..5202
    Translation

    Target ORF information:

    RefSeq Version XM_025149841.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150224
    Clone ID Related Accession (Same CDS sequence) XM_025149841.1 , XM_025149839.1 , XM_025149840.1
    Accession Version XM_025149839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005607.1
    CDS476..5272
    Translation

    Target ORF information:

    RefSeq Version XM_025149839.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150224
    Clone ID Related Accession (Same CDS sequence) XM_025149841.1 , XM_025149839.1 , XM_025149840.1
    Accession Version XM_025149840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005608.1
    CDS431..5227
    Translation

    Target ORF information:

    RefSeq Version XM_025149840.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAAAGG AAACAGAGGA TATCGAGAGC CCAAAGCGCA GCATTCGGGA CAGCGGCTAC 
    ATAGACTGCT GGGACTCGGA GCGCAGTGAT TCCCTCTCCC CCCCGCGCCA CGGCAGAGAT
    GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCCCCAGAT
    GTAGTGCTCA GGGGAAGCAG TGATGGGAGA GGAAGTGACT CAGAAACTGA CCTGCCACAC
    AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACACGGTGAA
    CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCAAACA AGAGCAATCA GACTTTCTAT
    GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTATCGAAA GAGCTGGAGC
    ACTGCGACTT CACCGCTGGG AGACAGACCT TTCAGCAAAC CTGAAACCAT AGAAGAAGAA
    CAAAGTGAAA CATCAGGACT GTGTGAAAAG GAGAAAATGG GCTCCATGGC TCCTGAGGAA
    AGCCCTGTTA AAATACAAGC AGATTTAAAT CAAAATGAAG GAGATTCCAT AGACCAGTCT
    CCTTCATGTA GTGTAAAAGA AACATCATCA CTCAAGGGAA AAGATGAAGC AGAGATCAGA
    AAGTTAAGAA GACTTGAACA AGCTGGCATT AAGGTCATGT CAGCTGCCCA GCGCTATGGC
    AGTAAAAAGC AAACATGTGA AGACAGAGGA AGCACCCCAG ACATCATCCT TCGCAAGGAA
    AACCCCTTCA TGACACGTTA TCAAGGGAAG GATTCTGGTT CAGAAGATGA AGCAGCAAGC
    AAAGTTCCTG ATTTAGAAAA GGATGACTTT GCCACCCGGA GAGCCAGGAT GAATCAGCCC
    AAACACGTAG TTCCATTTAA CTCCTTTATA ATTGGACCCT ATACGCAGAA AGATAAAAGT
    AAACTGGAAG ACAATAAAAA GCCATTGAAG GTGGAAAAGC AGGAACATGC AAGAGGAAGC
    CAGGTGCACA TGGCATCAGC AAGACAAACA GTGACATGCA AAGAGAACCC ACTTCTGCAG
    ACTGAGAAGA ATAAGTCAGA GGAGGAAGAG CAGGTGAAAG TAGTTGATGC CGAGAGAGAT
    GATTTGGCTA AACGGAAATC TCAGTGTGTC CTTCCTCAGC TACAAGAAGA TAGGATTTTT
    GTGAATGCCT CTATAACAAA AGCAGATCTG GAGACATGGA AAAGATTGAA ACTGTCAGGA
    GAGCCCAGTG AAGAAGATTG CAAGTGTCCC AGTGACACTC TACCAGCAGC TGAAGTCACA
    GTGCAAGCAG CAGTCCAGGA TGATTTTGCT GTCCGCAAAG CCAGAGCAAA CAGAAAGGCT
    GGTAGCCTCA AGCAGAAGTT TGTGCATTTT GGGCCAGTAA CTGAGATTGA TCAGCAGAAG
    TGGGACAAGC TCAGCATCGG CAGAGCTTCA CGCAGGTGTG AAGCAGAAGA GGAAAGCGGG
    AAAGACGGAG TGAAGGTTCA CTCGGATGTT GAAAGCTCTC TTCCAACCAC TGCTGGCAGT
    CTCCAGTTTG CTGAGCATGG TGATGCAGTA TCTAAGCCAC AGAATGAGCC CAGTGAGGTA
    GCGCAGTCAG ATCCAGCTGA ATGTTGCAGG AGCATGCCGT CACCTGCCGC TACTATTGAT
    GTCATCTCTG AGAAAGAAGC CCTGTGCAGC TCGAACAATC AGCAAGAAAG CGCCGAAGAA
    GAAGAAAGCA GTGCAAATTT ACCCGACATT GAGAGAGACG ATATGTTGGT TAGAAGGATG
    GGGACCTTTC AGAGGCGCAC AACTAGCACT GTTCCAATCC ACTGTCCTCC CCTGTCAGTA
    GCTCAGGAGA AGGATTTGAA GACTCCACGG GGAGCAGAGA GTACTGTAAC GCGAGAAGAG
    AGCCAGGAAC AGCTTGAAAT CCCTCATCAG CAGAGTGGCG GAACAGAAAA CAAAGTGAAG
    TTTCCAGACC CACAAAGAGA TGACATGTTG GCCAGAAGAA CAGGAGCTTT TCTAAAACAG
    CCGGGGCCAA GTGTTAAGCA GTTCCTTCCC ATGCCATATT CAAAGCAGCA GAATAAACAG
    GGCACAAGTA GAGAACTTGA AAAGAAAACT GCAAGGCCTG AAAAATGCCC ACAAGTCTGC
    ATTACTAGCT CTGGAAAACC TGTAATTCTA CCTCAGCAAG AGCAGCAACA AGGGCAGGAA
    AGATGCAGTC TCCCTCCTGC AGGACAAGAG GATACTGTGT GCACTGCCCA AGCGGGTGAT
    CATAGCCTTA ACGAAGTCCA CCTTGATCAA ACATCTAAGC TTCAGGTGGT TCCTGAGGAA
    AACAGAACTG AAACATTGTC ATCCAAGGAT GAGCATCTAC AAACATTCGG CCCCAGAACT
    GCTGTGTCTG ATGACGCAGA GAGCGTGAGC ATGTTTGACA TGAGGAGTGA GGAAGAAGCC
    ATGGCTCAGC CTCACAGCAA AGCTCGCCAT GAAAAGCTGC AGAATATACA CAACCAGCTG
    AAAGAGGACG AGACCAGGTG GCAAGATGAC CTGGCTCGGT GGAAGAGTCG TCGAAGGAGT
    GCTTCCCAGG ATTTAATTAA AAAAGAAGCT GAGAGAAAGA AAATGGAAAG ATTATTGTCT
    GGAGAGGGAA CAAATGAGCG CAGAAAAAGC ATCAAAACCT ACCGAGAAAT TGTGGAAGAG
    AAAGAGAGAA GAGAACGGGA GCTTCATGAG GCATACAAGA ATGCAAAGAC CCAGGAGGAG
    GCTGAGAGCA TACTCCAGCA GTACATCGAA AGATTCACCA TTAGCGAAGC TGTCCTTGAG
    CGTTTGCAGA TGCCAAAAAT CCTGGAAAGA AGCCATTCAG TGGAGCCAAA TTCCCCACTG
    AAAGACCCGA ATCCTCTGAG ATACTTAAGG CAACAATCAC TGCCTGCTCC AAAATTCACT
    GCCACCATTG AAGCAACCAT TGTTCCCACC ACTGAATTAG AGCCCAGTGG TTCGACAGGC
    CGCACATCTC CCAGCAAAAG TGTTGTCTCC AAGGCTGTGC CTATGCTGAC ACCCAAGCCT
    TACTCTCAAC CCAAAAATAC ACAGGAAGTT TTAAAGAACT TTAAGGTAGA TGGAAAAGTC
    AGCATGAATG GAGAGAATTT CAACGGAGTT GAAGAGAAGG ATAAAGAGTG TACAACAGTA
    ATATTTACTC AATCGCCAAG CAGATCTCTG AAATTTGATG GAGTAGCCAG AGGGGACGGG
    CCCCCCATAG AGTTGAAGAA GGACCCCACA AGTGTTGAGC TCAGTTTACA GAGGCCTGCC
    ACTCAGAGTG TGCACAGAGA GCCAACAGCC TTGCCGGGGG AAGCAGATCC GGCCGCCAGC
    CAGCAGGCCG AGGGCAGCCC TGGAGCAGTG GGACACGGGA GCCCGGCAGA CCTGTCCCCA
    GGTTCAGAAC AAAAGCAATT CAAGTCAACT GTACTTTGTG CCAGCCCTGT TGTGAAGAGC
    TTAGAATTTT CTCCTAGTCC TGTCCCTCCA GAGGAAACTG CTCCAAGTGA GAAAGATGTG
    AAGAAACCTG AAATGGAGAA GCAGAAAGAG GCAGCACTCC CTGCTATGCA GGAGGTAATG
    AAACCGAAAG CCCTAGAACA AGAAGGGAGT GTAGTATTGC CATTTTTGAA GAAACTGCCA
    GAGACCAGCC AGGTCACTCT GCATAATTCT GATCTGCAAG AGCCACCCCG CAGGGTCATG
    GTTCCGCTCA GGGTGCGGAA CGACTGGCGG CGCTCCCGGT TTTTCTCACA GCCAGCTGAT
    TCTGGCAATA GTGACAAATC AAATCGTTTC AATTTCTGGT CCTGGGACCC AGAGGAAGAG
    CGGAAGCGGC AGGAGAGGTG GCAGCATGAG CAAGAACGCC TGCTTCAGGA GAAGTATCAG
    AAGGAGCAAG ACAAACTGAA GGAGGAATGG GAAAAGGCAC AGAGAGAAGT TGAAGAGGAG
    GAGCGTAGAT ACTATGAGGA GGAACGTAAG ATAATCGAGG ACACTGTAGT TCCATTCATT
    GTTTCTTCAA ACTCTGCAGA ACTCCTCTCC TCTTCTTCCT CTACCACTGA AGGAAATAAA
    ACAGCGAACA CAAGTGATTC AAATGGCTCT CCAGAGGAAG GCAAACCAAA AGAAGTTACA
    AGGCAGAAAA AGCTGGTTTG GGAGCAGGAC AGCATAGCAT CTCTGAAAAA CAAGGAAAAT
    GAACGCTGGC AAGAAAAGAG GAGCAGTGGA AATAAAGTTC TCTCAGGACA CCCAGAGGTG
    GGTATCTTGA AAGGAGGTGA CCAGGAGTAC CAGGTGCCGG TGATGGAGCG CTGCTCGCCT
    GCCCGGTTGA ATCTGCCATT GACTCAGGAT GTCCCATGGA ACCAGCAGCT GCTAACGAAG
    CAGTCCCCAC ACCACGCAGA GGACGCCCGG CAGAGACCGC TGCTGGGGCA GAGTGCAGGA
    CAGCAGCCGA GCGCCGCAGG GGAAGGGAGG AGGGCAGGCT GTCATGAGAA CTTTCGATCT
    GGGCCATCGT CTCCCTGCTC ACCTGCTGCA CCAAGTCAGT CACCCAACAG GTACAAAAGG
    TCTGTCAGCG GAAAGAAGCT TTGTTCCACC TGTGGGCTTC CTCTTGGCAA GGGAGCTGCC
    ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GTTTCAGGTG CGGCATTTGC
    AAGGGACAGC TGGGAGATGC AGCAAGTGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTG
    AACTGCAATG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35480
    Clone ID Related Accession (Same CDS sequence) XM_015285462.2 , XM_015285462.1
    Accession Version XM_015285462.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT
    GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG
    ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG
    GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC
    TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140948.1
    CDS158..5431
    Translation

    Target ORF information:

    RefSeq Version XM_015285462.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT
    GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG
    ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG
    GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC
    TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35480
    Clone ID Related Accession (Same CDS sequence) XM_015285462.2 , XM_015285462.1
    Accession Version XM_015285462.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT
    GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG
    ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG
    GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC
    TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140948.1
    CDS157..5430
    Translation

    Target ORF information:

    RefSeq Version XM_015285462.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTA CAAAAGGTCT
    GTCAGCGGAA AGAAGCTTTG TTCCACCTGT GGGCTTCCTC TTGGCAAGGG AGCTGCCATG
    ATTATTGAAA CGCTTGGTCT TTATTTCCAT ATTCAGTGTT TCAGGTGCGG CATTTGCAAG
    GGACAGCTGG GAGATGCAGC AAGTGGGACA GATGTCAGGA TTAGAAATGG TCTTCTGAAC
    TGCAATGATT GTTATATCCG ATCCAGAACT GCTGGACAGC CAACTACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35481
    Clone ID Related Accession (Same CDS sequence) XM_015285463.1 , XM_015285463.2
    Accession Version XM_015285463.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC
    AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT
    ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA
    CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC
    AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140949.1
    CDS158..5428
    Translation

    Target ORF information:

    RefSeq Version XM_015285463.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC
    AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT
    ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA
    CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC
    AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35481
    Clone ID Related Accession (Same CDS sequence) XM_015285463.1 , XM_015285463.2
    Accession Version XM_015285463.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC
    AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT
    ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA
    CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC
    AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140949.1
    CDS159..5429
    Translation

    Target ORF information:

    RefSeq Version XM_015285463.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285463.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTACAA AAGGTCTGTC
    AGCGGAAAGA AGCTTTGTTC CACCTGTGGG CTTCCTCTTG GCAAGGGAGC TGCCATGATT
    ATTGAAACGC TTGGTCTTTA TTTCCATATT CAGTGTTTCA GGTGCGGCAT TTGCAAGGGA
    CAGCTGGGAG ATGCAGCAAG TGGGACAGAT GTCAGGATTA GAAATGGTCT TCTGAACTGC
    AATGATTGTT ATATCCGATC CAGAACTGCT GGACAGCCAA CTACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35482
    Clone ID Related Accession (Same CDS sequence) XM_015285464.1
    Accession Version XM_015285464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTC TGTCAGCGGA
    AAGAAGCTTT GTTCCACCTG TGGGCTTCCT CTTGGCAAGG GAGCTGCCAT GATTATTGAA
    ACGCTTGGTC TTTATTTCCA TATTCAGTGT TTCAGGTGCG GCATTTGCAA GGGACAGCTG
    GGAGATGCAG CAAGTGGGAC AGATGTCAGG ATTAGAAATG GTCTTCTGAA CTGCAATGAT
    TGTTATATCC GATCCAGAAC TGCTGGACAG CCAACTACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140950.1
    CDS157..5421
    Translation

    Target ORF information:

    RefSeq Version XM_015285464.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG CAGTGGAAAT AAAGTTCTCT CAGGACACCC AGAGGTGGGT
    ATCTTGAAAG GAGGTGACCA GGAGTACCAG GTGCCGGTGA TGGAGCGCTG CTCGCCTGCC
    CGGTTGAATC TGCCATTGAC TCAGGATGTC CCATGGAACC AGCAGCTGCT AACGAAGCAG
    TCCCCACACC ACGCAGAGGA CGCCCGGCAG AGACCGCTGC TGGGGCAGAG TGCAGGACAG
    CAGCCGAGCG CCGCAGGGGA AGGGAGGAGG GCAGGCTGTC ATGAGAACTT TCGATCTGGG
    CCATCGTCTC CCTGCTCACC TGCTGCACCA AGTCAGTCAC CCAACAGGTC TGTCAGCGGA
    AAGAAGCTTT GTTCCACCTG TGGGCTTCCT CTTGGCAAGG GAGCTGCCAT GATTATTGAA
    ACGCTTGGTC TTTATTTCCA TATTCAGTGT TTCAGGTGCG GCATTTGCAA GGGACAGCTG
    GGAGATGCAG CAAGTGGGAC AGATGTCAGG ATTAGAAATG GTCTTCTGAA CTGCAATGAT
    TGTTATATCC GATCCAGAAC TGCTGGACAG CCAACTACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35483
    Clone ID Related Accession (Same CDS sequence) XM_015285465.1
    Accession Version XM_015285465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTCTGT CAGCGGAAAG
    AAGCTTTGTT CCACCTGTGG GCTTCCTCTT GGCAAGGGAG CTGCCATGAT TATTGAAACG
    CTTGGTCTTT ATTTCCATAT TCAGTGTTTC AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA
    GATGCAGCAA GTGGGACAGA TGTCAGGATT AGAAATGGTC TTCTGAACTG CAATGATTGT
    TATATCCGAT CCAGAACTGC TGGACAGCCA ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140951.1
    CDS158..5419
    Translation

    Target ORF information:

    RefSeq Version XM_015285465.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGAGC CACCCCGCAG GGTCATGGTT
    CCGCTCAGGG TGCGGAACGA CTGGCGGCGC TCCCGGTTTT TCTCACAGCC AGCTGATTCT
    GGCAATAGTG ACAAATCAAA TCGTTTCAAT TTCTGGTCCT GGGACCCAGA GGAAGAGCGG
    AAGCGGCAGG AGAGGTGGCA GCATGAGCAA GAACGCCTGC TTCAGGAGAA GTATCAGAAG
    GAGCAAGACA AACTGAAGGA GGAATGGGAA AAGGCACAGA GAGAAGTTGA AGAGGAGGAG
    CGTAGATACT ATGAGGAGGA ACGTAAGATA ATCGAGGACA CTGTAGTTCC ATTCATTGTT
    TCTTCAAACT CTGCAGAACT CCTCTCCTCT TCTTCCTCTA CCACTGAAGG AAATAAAACA
    GCGAACACAA GTGATTCAAA TGGCTCTCCA GAGGAAGGCA AACCAAAAGA AGTTACAAGG
    CAGAAAAAGC TGGTTTGGGA GCAGGACAGC ATAGCATCTC TGAAAAACAA GGAAAATGAA
    CGCTGGCAAG AAAAGAGGAG TGGAAATAAA GTTCTCTCAG GACACCCAGA GGTGGGTATC
    TTGAAAGGAG GTGACCAGGA GTACCAGGTG CCGGTGATGG AGCGCTGCTC GCCTGCCCGG
    TTGAATCTGC CATTGACTCA GGATGTCCCA TGGAACCAGC AGCTGCTAAC GAAGCAGTCC
    CCACACCACG CAGAGGACGC CCGGCAGAGA CCGCTGCTGG GGCAGAGTGC AGGACAGCAG
    CCGAGCGCCG CAGGGGAAGG GAGGAGGGCA GGCTGTCATG AGAACTTTCG ATCTGGGCCA
    TCGTCTCCCT GCTCACCTGC TGCACCAAGT CAGTCACCCA ACAGGTCTGT CAGCGGAAAG
    AAGCTTTGTT CCACCTGTGG GCTTCCTCTT GGCAAGGGAG CTGCCATGAT TATTGAAACG
    CTTGGTCTTT ATTTCCATAT TCAGTGTTTC AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA
    GATGCAGCAA GTGGGACAGA TGTCAGGATT AGAAATGGTC TTCTGAACTG CAATGATTGT
    TATATCCGAT CCAGAACTGC TGGACAGCCA ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35484
    Clone ID Related Accession (Same CDS sequence) XM_015285466.1 , XM_015285466.2
    Accession Version XM_015285466.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA
    GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG
    GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC
    CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA
    AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT
    ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT
    TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA
    CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC
    TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG
    GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT
    CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA
    GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT
    GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC
    ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA
    CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG
    AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA
    GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG
    GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG
    CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG
    GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT
    GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC
    AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT
    GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC
    AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT
    AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140952.1
    CDS158..5209
    Translation

    Target ORF information:

    RefSeq Version XM_015285466.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA
    GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG
    GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC
    CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA
    AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT
    ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT
    TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA
    CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC
    TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG
    GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT
    CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA
    GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT
    GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC
    ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA
    CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG
    AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA
    GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG
    GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG
    CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG
    GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT
    GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC
    AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT
    GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC
    AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT
    AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35484
    Clone ID Related Accession (Same CDS sequence) XM_015285466.1 , XM_015285466.2
    Accession Version XM_015285466.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA
    GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG
    GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC
    CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA
    AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT
    ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT
    TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA
    CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC
    TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG
    GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT
    CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA
    GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT
    GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC
    ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA
    CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG
    AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA
    GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG
    GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG
    CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG
    GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT
    GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC
    AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT
    GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC
    AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT
    AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140952.1
    CDS159..5210
    Translation

    Target ORF information:

    RefSeq Version XM_015285466.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285466.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGCCTTGCC GGGGGAAGCA
    GATCCGGCCG CCAGCCAGCA GGCCGAGGGC AGCCCTGGAG CAGTGGGACA CGGGAGCCCG
    GCAGACCTGT CCCCAGGTTC AGAACAAAAG CAATTCAAGT CAACTGTACT TTGTGCCAGC
    CCTGTTGTGA AGAGCTTAGA ATTTTCTCCT AGTCCTGTCC CTCCAGAGGA AACTGCTCCA
    AGTGAGAAAG ATGTGAAGAA ACCTGAAATG GAGAAGCAGA AAGAGGCAGC ACTCCCTGCT
    ATGCAGGAGG TAATGAAACC GAAAGCCCTA GAACAAGAAG GGAGTGTAGT ATTGCCATTT
    TTGAAGAAAC TGCCAGAGAC CAGCCAGGTC ACTCTGCATA ATTCTGATCT GCAAGAGCCA
    CCCCGCAGGG TCATGGTTCC GCTCAGGGTG CGGAACGACT GGCGGCGCTC CCGGTTTTTC
    TCACAGCCAG CTGATTCTGG CAATAGTGAC AAATCAAATC GTTTCAATTT CTGGTCCTGG
    GACCCAGAGG AAGAGCGGAA GCGGCAGGAG AGGTGGCAGC ATGAGCAAGA ACGCCTGCTT
    CAGGAGAAGT ATCAGAAGGA GCAAGACAAA CTGAAGGAGG AATGGGAAAA GGCACAGAGA
    GAAGTTGAAG AGGAGGAGCG TAGATACTAT GAGGAGGAAC GTAAGATAAT CGAGGACACT
    GTAGTTCCAT TCATTGTTTC TTCAAACTCT GCAGAACTCC TCTCCTCTTC TTCCTCTACC
    ACTGAAGGAA ATAAAACAGC GAACACAAGT GATTCAAATG GCTCTCCAGA GGAAGGCAAA
    CCAAAAGAAG TTACAAGGCA GAAAAAGCTG GTTTGGGAGC AGGACAGCAT AGCATCTCTG
    AAAAACAAGG AAAATGAACG CTGGCAAGAA AAGAGGAGCA GTGGAAATAA AGTTCTCTCA
    GGACACCCAG AGGTGGGTAT CTTGAAAGGA GGTGACCAGG AGTACCAGGT GCCGGTGATG
    GAGCGCTGCT CGCCTGCCCG GTTGAATCTG CCATTGACTC AGGATGTCCC ATGGAACCAG
    CAGCTGCTAA CGAAGCAGTC CCCACACCAC GCAGAGGACG CCCGGCAGAG ACCGCTGCTG
    GGGCAGAGTG CAGGACAGCA GCCGAGCGCC GCAGGGGAAG GGAGGAGGGC AGGCTGTCAT
    GAGAACTTTC GATCTGGGCC ATCGTCTCCC TGCTCACCTG CTGCACCAAG TCAGTCACCC
    AACAGGTACA AAAGGTCTGT CAGCGGAAAG AAGCTTTGTT CCACCTGTGG GCTTCCTCTT
    GGCAAGGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGTTTC
    AGGTGCGGCA TTTGCAAGGG ACAGCTGGGA GATGCAGCAA GTGGGACAGA TGTCAGGATT
    AGAAATGGTC TTCTGAACTG CAATGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA
    ACTACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35485
    Clone ID Related Accession (Same CDS sequence) XM_015285467.1
    Accession Version XM_015285467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG TGAAGAAGAT
    TGCAAGTGTC CCAGTGACAC TCTACCAGCA GCTGAAGTCA CAGTGCAAGC AGCAGTCCAG
    GATGATTTTG CTGTCCGCAA AGCCAGAGCA AACAGAAAGG CTGGTAGCCT CAAGCAGAAG
    TTTGTGCATT TTGGGCCAGT AACTGAGATT GATCAGCAGA AGTGGGACAA GCTCAGCATC
    GGCAGAGCTT CACGCAGGTG TGAAGCAGAA GAGGAAAGCG GGAAAGACGG AGTGAAGGTT
    CACTCGGATG TTGAAAGCTC TCTTCCAACC ACTGCTGGCA GTCTCCAGTT TGCTGAGCAT
    GGTGATGCAG TATCTAAGCC ACAGAATGAG CCCAGTGAGG TAGCGCAGTC AGATCCAGCT
    GAATGTTGCA GGAGCATGCC GTCACCTGCC GCTACTATTG ATGTCATCTC TGAGAAAGAA
    GCCCTGTGCA GCTCGAACAA TCAGCAAGAA AGCGCCGAAG AAGAAGAAAG CAGTGCAAAT
    TTACCCGACA TTGAGAGAGA CGATATGTTG GTTAGAAGGA TGGGGACCTT TCAGAGGCGC
    ACAACTAGCA CTGTTCCAAT CCACTGTCCT CCCCTGTCAG TAGCTCAGGA GAAGGATTTG
    AAGACTCCAC GGGGAGCAGA GAGTACTGTA ACGCGAGAAG AGAGCCAGGA ACAGCTTGAA
    ATCCCTCATC AGCAGAGTGG CGGAACAGAA AACAAAGTGA AGTTTCCAGA CCCACAAAGA
    GATGACATGT TGGCCAGAAG AACAGGAGCT TTTCTAAAAC AGCCGGGGCC AAGTGTTAAG
    CAGTTCCTTC CCATGCCATA TTCAAAGCAG CAGAATAAAC AGGGCACAAG TAGAGAACTT
    GAAAAGAAAA CTGCAAGGCC TGAAAAATGC CCACAAGTCT GCATTACTAG CTCTGGAAAA
    CCTGTAATTC TACCTCAGCA AGAGCAGCAA CAAGGGCAGG AAAGATGCAG TCTCCCTCCT
    GCAGGACAAG AGGATACTGT GTGCACTGCC CAAGCGGGTG ATCATAGCCT TAACGAAGTC
    CACCTTGATC AAACATCTAA GCTTCAGGTG GTTCCTGAGG AAAACAGAAC TGAAACATTG
    TCATCCAAGG ATGAGCATCT ACAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA
    GAGAGCGTGA GCATGTTTGA CATGAGGAGT GAGGAAGAAG CCATGGCTCA GCCTCACAGC
    AAAGCTCGCC ATGAAAAGCT GCAGAATATA CACAACCAGC TGAAAGAGGA CGAGACCAGG
    TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GTGCTTCCCA GGATTTAATT
    AAAAAAGAAG CTGAGAGAAA GAAAATGGAA AGATTATTGT CTGGAGAGGG AACAAATGAG
    CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAACGG
    GAGCTTCATG AGGCATACAA GAATGCAAAG ACCCAGGAGG AGGCTGAGAG CATACTCCAG
    CAGTACATCG AAAGATTCAC CATTAGCGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA
    ATCCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC GAATCCTCTG
    AGATACTTAA GGCAACAATC ACTGCCTGCT CCAAAATTCA CTGCCACCAT TGAAGCAACC
    ATTGTTCCCA CCACTGAATT AGAGCCCAGT GGTTCGACAG GCCGCACATC TCCCAGCAAA
    AGTGTTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCTCA ACCCAAAAAT
    ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAGAAT
    TTCAACGGAG TTGAAGAGAA GGATAAAGAG TGTACAACAG TAATATTTAC TCAATCGCCA
    AGCAGATCTC TGAAATTTGA TGGAGTAGCC AGAGGGGACG GGCCCCCCAT AGAGTTGAAG
    AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAGA
    GAGCCAACAG CCTTGCCGGG GGAAGCAGAT CCGGCCGCCA GCCAGCAGGC CGAGGGCAGC
    CCTGGAGCAG TGGGACACGG GAGCCCGGCA GACCTGTCCC CAGGTTCAGA ACAAAAGCAA
    TTCAAGTCAA CTGTACTTTG TGCCAGCCCT GTTGTGAAGA GCTTAGAATT TTCTCCTAGT
    CCTGTCCCTC CAGAGGAAAC TGCTCCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG
    AAGCAGAAAG AGGCAGCACT CCCTGCTATG CAGGAGGTAA TGAAACCGAA AGCCCTAGAA
    CAAGAAGGGA GTGTAGTATT GCCATTTTTG AAGAAACTGC CAGAGACCAG CCAGGTCACT
    CTGCATAATT CTGATCTGCA AGAGCCACCC CGCAGGGTCA TGGTTCCGCT CAGGGTGCGG
    AACGACTGGC GGCGCTCCCG GTTTTTCTCA CAGCCAGCTG ATTCTGGCAA TAGTGACAAA
    TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG
    TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG
    AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG
    GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA
    GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT
    TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT
    TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG
    AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT
    GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA
    TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA
    GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA
    GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC
    TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG
    CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT
    GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT
    GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT
    ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140953.1
    CDS157..5175
    Translation

    Target ORF information:

    RefSeq Version XM_015285467.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG TGAAGAAGAT
    TGCAAGTGTC CCAGTGACAC TCTACCAGCA GCTGAAGTCA CAGTGCAAGC AGCAGTCCAG
    GATGATTTTG CTGTCCGCAA AGCCAGAGCA AACAGAAAGG CTGGTAGCCT CAAGCAGAAG
    TTTGTGCATT TTGGGCCAGT AACTGAGATT GATCAGCAGA AGTGGGACAA GCTCAGCATC
    GGCAGAGCTT CACGCAGGTG TGAAGCAGAA GAGGAAAGCG GGAAAGACGG AGTGAAGGTT
    CACTCGGATG TTGAAAGCTC TCTTCCAACC ACTGCTGGCA GTCTCCAGTT TGCTGAGCAT
    GGTGATGCAG TATCTAAGCC ACAGAATGAG CCCAGTGAGG TAGCGCAGTC AGATCCAGCT
    GAATGTTGCA GGAGCATGCC GTCACCTGCC GCTACTATTG ATGTCATCTC TGAGAAAGAA
    GCCCTGTGCA GCTCGAACAA TCAGCAAGAA AGCGCCGAAG AAGAAGAAAG CAGTGCAAAT
    TTACCCGACA TTGAGAGAGA CGATATGTTG GTTAGAAGGA TGGGGACCTT TCAGAGGCGC
    ACAACTAGCA CTGTTCCAAT CCACTGTCCT CCCCTGTCAG TAGCTCAGGA GAAGGATTTG
    AAGACTCCAC GGGGAGCAGA GAGTACTGTA ACGCGAGAAG AGAGCCAGGA ACAGCTTGAA
    ATCCCTCATC AGCAGAGTGG CGGAACAGAA AACAAAGTGA AGTTTCCAGA CCCACAAAGA
    GATGACATGT TGGCCAGAAG AACAGGAGCT TTTCTAAAAC AGCCGGGGCC AAGTGTTAAG
    CAGTTCCTTC CCATGCCATA TTCAAAGCAG CAGAATAAAC AGGGCACAAG TAGAGAACTT
    GAAAAGAAAA CTGCAAGGCC TGAAAAATGC CCACAAGTCT GCATTACTAG CTCTGGAAAA
    CCTGTAATTC TACCTCAGCA AGAGCAGCAA CAAGGGCAGG AAAGATGCAG TCTCCCTCCT
    GCAGGACAAG AGGATACTGT GTGCACTGCC CAAGCGGGTG ATCATAGCCT TAACGAAGTC
    CACCTTGATC AAACATCTAA GCTTCAGGTG GTTCCTGAGG AAAACAGAAC TGAAACATTG
    TCATCCAAGG ATGAGCATCT ACAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA
    GAGAGCGTGA GCATGTTTGA CATGAGGAGT GAGGAAGAAG CCATGGCTCA GCCTCACAGC
    AAAGCTCGCC ATGAAAAGCT GCAGAATATA CACAACCAGC TGAAAGAGGA CGAGACCAGG
    TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GTGCTTCCCA GGATTTAATT
    AAAAAAGAAG CTGAGAGAAA GAAAATGGAA AGATTATTGT CTGGAGAGGG AACAAATGAG
    CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAACGG
    GAGCTTCATG AGGCATACAA GAATGCAAAG ACCCAGGAGG AGGCTGAGAG CATACTCCAG
    CAGTACATCG AAAGATTCAC CATTAGCGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA
    ATCCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC GAATCCTCTG
    AGATACTTAA GGCAACAATC ACTGCCTGCT CCAAAATTCA CTGCCACCAT TGAAGCAACC
    ATTGTTCCCA CCACTGAATT AGAGCCCAGT GGTTCGACAG GCCGCACATC TCCCAGCAAA
    AGTGTTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCTCA ACCCAAAAAT
    ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAGAAT
    TTCAACGGAG TTGAAGAGAA GGATAAAGAG TGTACAACAG TAATATTTAC TCAATCGCCA
    AGCAGATCTC TGAAATTTGA TGGAGTAGCC AGAGGGGACG GGCCCCCCAT AGAGTTGAAG
    AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAGA
    GAGCCAACAG CCTTGCCGGG GGAAGCAGAT CCGGCCGCCA GCCAGCAGGC CGAGGGCAGC
    CCTGGAGCAG TGGGACACGG GAGCCCGGCA GACCTGTCCC CAGGTTCAGA ACAAAAGCAA
    TTCAAGTCAA CTGTACTTTG TGCCAGCCCT GTTGTGAAGA GCTTAGAATT TTCTCCTAGT
    CCTGTCCCTC CAGAGGAAAC TGCTCCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG
    AAGCAGAAAG AGGCAGCACT CCCTGCTATG CAGGAGGTAA TGAAACCGAA AGCCCTAGAA
    CAAGAAGGGA GTGTAGTATT GCCATTTTTG AAGAAACTGC CAGAGACCAG CCAGGTCACT
    CTGCATAATT CTGATCTGCA AGAGCCACCC CGCAGGGTCA TGGTTCCGCT CAGGGTGCGG
    AACGACTGGC GGCGCTCCCG GTTTTTCTCA CAGCCAGCTG ATTCTGGCAA TAGTGACAAA
    TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG
    TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG
    AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG
    GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA
    GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT
    TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT
    TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG
    AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT
    GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA
    TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA
    GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA
    GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC
    TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG
    CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT
    GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT
    GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT
    ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35486
    Clone ID Related Accession (Same CDS sequence) XM_015285468.2 , XM_015285468.1
    Accession Version XM_015285468.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
    AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC
    CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG
    CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC
    TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG
    AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC
    AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA
    GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA
    CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG
    GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA
    GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA
    AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG
    TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA
    GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT
    TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG
    AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC
    TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG
    CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC
    AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA
    GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG
    CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG
    AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT
    CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA
    GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA
    ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT
    GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT
    CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA
    CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG
    TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT
    CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC
    GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC
    CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG
    AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC
    GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA
    ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT
    TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT
    GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA
    GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG
    TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG
    CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA
    CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC
    ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG
    CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG
    TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG
    AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC
    TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG
    AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC
    ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA
    GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA
    CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA
    GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG
    CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC
    TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG
    GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT
    GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG
    CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG
    GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG
    AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT
    GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT
    GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC
    CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG
    CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA
    GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG
    TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG
    TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC
    CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA
    CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG
    GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC
    TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA
    GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA
    TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT
    CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG
    GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG
    AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG
    CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC
    TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG
    TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT
    CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG
    TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC
    AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA
    CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140954.1
    CDS108..4925
    Translation

    Target ORF information:

    RefSeq Version XM_015285468.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285468.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
    AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC
    CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG
    CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC
    TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG
    AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC
    AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA
    GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA
    CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG
    GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA
    GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA
    AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG
    TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA
    GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT
    TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG
    AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC
    TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG
    CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC
    AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA
    GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG
    CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG
    AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT
    CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA
    GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA
    ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT
    GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT
    CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA
    CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG
    TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT
    CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC
    GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC
    CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG
    AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC
    GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA
    ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT
    TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT
    GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA
    GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG
    TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG
    CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA
    CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC
    ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG
    CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG
    TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG
    AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC
    TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG
    AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC
    ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA
    GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA
    CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA
    GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG
    CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC
    TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG
    GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT
    GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG
    CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG
    GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG
    AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT
    GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT
    GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC
    CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG
    CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA
    GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG
    TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG
    TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC
    CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA
    CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG
    GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC
    TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA
    GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA
    TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT
    CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG
    GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG
    AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG
    CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC
    TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG
    TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT
    CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG
    TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC
    AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA
    CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35486
    Clone ID Related Accession (Same CDS sequence) XM_015285468.2 , XM_015285468.1
    Accession Version XM_015285468.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
    AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC
    CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG
    CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC
    TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG
    AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC
    AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA
    GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA
    CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG
    GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA
    GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA
    AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG
    TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA
    GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT
    TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG
    AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC
    TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG
    CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC
    AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA
    GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG
    CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG
    AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT
    CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA
    GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA
    ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT
    GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT
    CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA
    CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG
    TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT
    CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC
    GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC
    CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG
    AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC
    GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA
    ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT
    TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT
    GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA
    GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG
    TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG
    CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA
    CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC
    ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG
    CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG
    TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG
    AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC
    TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG
    AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC
    ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA
    GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA
    CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA
    GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG
    CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC
    TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG
    GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT
    GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG
    CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG
    GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG
    AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT
    GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT
    GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC
    CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG
    CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA
    GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG
    TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG
    TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC
    CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA
    CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG
    GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC
    TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA
    GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA
    TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT
    CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG
    GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG
    AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG
    CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC
    TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG
    TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT
    CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG
    TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC
    AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA
    CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140954.1
    CDS124..4941
    Translation

    Target ORF information:

    RefSeq Version XM_015285468.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGACTATG AACGACAAGT CACATTTCAG GAAACAGAGG ATATCGAGAG CCCAAAGCGC 
    AGCATTCGGG ACAGCGGCTA CATAGACTGC TGGGACTCGG AGCGCAGTGA TTCCCTCTCC
    CCCCCGCGCC ACGGCAGAGA TGATTCCTTT GACAGTCTGG ACTCCTTTGG CTCCCGCTCG
    CAGCAGACAC CGTCCCCAGA TGTAGTGCTC AGGGGAAGCA GTGATGGGAG AGGAAGTGAC
    TCAGAAACTG ACCTGCCACA CAGAAAGATG CCAGATGTGA AAAAAGATGA TATGTTGGCG
    AGGCGGACCT CACACGGTGA ACCTAAATCA GCTGTGCCTT TTAACCAGTA CCTCCCAAAC
    AAGAGCAATC AGACTTTCTA TGTACCTGCT CCACTTCGGA AAAAGAAAGC TGAACGAGAA
    GAGTATCGAA AGAGCTGGAG CACTGCGACT TCACCGCTGG GAGACAGACC TTTCAGCAAA
    CCTGAAACCA TAGAAGAAGA ACAAAGTGAA ACATCAGGAC TGTGTGAAAA GGAGAAAATG
    GGCTCCATGG CTCCTGAGGA AAGCCCTGTT AAAATACAAG CAGATTTAAA TCAAAATGAA
    GGAGATTCCA TAGACCAGTC TCCTTCATGT AGTGTAAAAG AAACATCATC ACTCAAGGGA
    AAAGATGAAG CAGAGATCAG AAAGTTAAGA AGACTTGAAC AAGCTGGCAT TAAGGTCATG
    TCAGCTGCCC AGCGCTATGG CAGTAAAAAG CAAACATGTG AAGACAGAGG AAGCACCCCA
    GACATCATCC TTCGCAAGGA AAACCCCTTC ATGACACGTT ATCAAGGGAA GGATTCTGGT
    TCAGAAGATG AAGCAGCAAG CAAAGTTCCT GATTTAGAAA AGGATGACTT TGCCACCCGG
    AGAGCCAGGA TGAATCAGCC CAAACACGTA GTTCCATTTA ACTCCTTTAT AATTGGACCC
    TATACGCAGA AAGATAAAAG TAAACTGGAA GACAATAAAA AGCCATTGAA GGTGGAAAAG
    CAGGAACATG CAAGAGGAAG CCAGGTGCAC ATGGCATCAG CAAGACAAAC AGTGACATGC
    AAAGAGAACC CACTTCTGCA GACTGAGAAG AATAAGTCAG AGGAGGAAGA GCAGGTGAAA
    GTAGTTGATG CCGAGAGAGA TGATTTGGCT AAACGGAAAT CTCAGTGTGT CCTTCCTCAG
    CTACAAGAAG ATAGGATTTT TGTGAATGCC TCTATAACAA AAGCAGATCT GGAGACATGG
    AAAAGATTGA AACTGTCAGG AGAGCCCAGT GAAGAAGATT GCAAGTGTCC CAGTGACACT
    CTACCAGCAG CTGAAGTCAC AGTGCAAGCA GCAGTCCAGG ATGATTTTGC TGTCCGCAAA
    GCCAGAGCAA ACAGAAAGGC TGGTAGCCTC AAGCAGAAGT TTGTGCATTT TGGGCCAGTA
    ACTGAGATTG ATCAGCAGAA GTGGGACAAG CTCAGCATCG GCAGAGCTTC ACGCAGGTGT
    GAAGCAGAAG AGGAAAGCGG GAAAGACGGA GTGAAGGTTC ACTCGGATGT TGAAAGCTCT
    CTTCCAACCA CTGCTGGCAG TCTCCAGTTT GCTGAGCATG GTGATGCAGT ATCTAAGCCA
    CAGAATGAGC CCAGTGAGGT AGCGCAGTCA GATCCAGCTG AATGTTGCAG GAGCATGCCG
    TCACCTGCCG CTACTATTGA TGTCATCTCT GAGAAAGAAG CCCTGTGCAG CTCGAACAAT
    CAGCAAGAAA GCGCCGAAGA AGAAGAAAGC AGTGCAAATT TACCCGACAT TGAGAGAGAC
    GATATGTTGG TTAGAAGGAT GGGGACCTTT CAGAGGCGCA CAACTAGCAC TGTTCCAATC
    CACTGTCCTC CCCTGTCAGT AGCTCAGGAG AAGGATTTGA AGACTCCACG GGGAGCAGAG
    AGTACTGTAA CGCGAGAAGA GAGCCAGGAA CAGCTTGAAA TCCCTCATCA GCAGAGTGGC
    GGAACAGAAA ACAAAGTGAA GTTTCCAGAC CCACAAAGAG ATGACATGTT GGCCAGAAGA
    ACAGGAGCTT TTCTAAAACA GCCGGGGCCA AGTGTTAAGC AGTTCCTTCC CATGCCATAT
    TCAAAGCAGC AGAATAAACA GGGCACAAGT AGAGAACTTG AAAAGAAAAC TGCAAGGCCT
    GAAAAATGCC CACAAGTCTG CATTACTAGC TCTGGAAAAC CTGTAATTCT ACCTCAGCAA
    GAGCAGCAAC AAGGGCAGGA AAGATGCAGT CTCCCTCCTG CAGGACAAGA GGATACTGTG
    TGCACTGCCC AAGCGGGTGA TCATAGCCTT AACGAAGTCC ACCTTGATCA AACATCTAAG
    CTTCAGGTGG TTCCTGAGGA AAACAGAACT GAAACATTGT CATCCAAGGA TGAGCATCTA
    CAAACATTCG GCCCCAGAAC TGCTGTGTCT GATGACGCAG AGAGCGTGAG CATGTTTGAC
    ATGAGGAGTG AGGAAGAAGC CATGGCTCAG CCTCACAGCA AAGCTCGCCA TGAAAAGCTG
    CAGAATATAC ACAACCAGCT GAAAGAGGAC GAGACCAGGT GGCAAGATGA CCTGGCTCGG
    TGGAAGAGTC GTCGAAGGAG TGCTTCCCAG GATTTAATTA AAAAAGAAGC TGAGAGAAAG
    AAAATGGAAA GATTATTGTC TGGAGAGGGA ACAAATGAGC GCAGAAAAAG CATCAAAACC
    TACCGAGAAA TTGTGGAAGA GAAAGAGAGA AGAGAACGGG AGCTTCATGA GGCATACAAG
    AATGCAAAGA CCCAGGAGGA GGCTGAGAGC ATACTCCAGC AGTACATCGA AAGATTCACC
    ATTAGCGAAG CTGTCCTTGA GCGTTTGCAG ATGCCAAAAA TCCTGGAAAG AAGCCATTCA
    GTGGAGCCAA ATTCCCCACT GAAAGACCCG AATCCTCTGA GATACTTAAG GCAACAATCA
    CTGCCTGCTC CAAAATTCAC TGCCACCATT GAAGCAACCA TTGTTCCCAC CACTGAATTA
    GAGCCCAGTG GTTCGACAGG CCGCACATCT CCCAGCAAAA GTGTTGTCTC CAAGGCTGTG
    CCTATGCTGA CACCCAAGCC TTACTCTCAA CCCAAAAATA CACAGGAAGT TTTAAAGAAC
    TTTAAGGTAG ATGGAAAAGT CAGCATGAAT GGAGAGAATT TCAACGGAGT TGAAGAGAAG
    GATAAAGAGT GTACAACAGT AATATTTACT CAATCGCCAA GCAGATCTCT GAAATTTGAT
    GGAGTAGCCA GAGGGGACGG GCCCCCCATA GAGTTGAAGA AGGACCCCAC AAGTGTTGAG
    CTCAGTTTAC AGAGGCCTGC CACTCAGAGT GTGCACAGAG AGCCAACAGC CTTGCCGGGG
    GAAGCAGATC CGGCCGCCAG CCAGCAGGCC GAGGGCAGCC CTGGAGCAGT GGGACACGGG
    AGCCCGGCAG ACCTGTCCCC AGGTTCAGAA CAAAAGCAAT TCAAGTCAAC TGTACTTTGT
    GCCAGCCCTG TTGTGAAGAG CTTAGAATTT TCTCCTAGTC CTGTCCCTCC AGAGGAAACT
    GCTCCAAGTG AGAAAGATGT GAAGAAACCT GAAATGGAGA AGCAGAAAGA GGCAGCACTC
    CCTGCTATGC AGGAGGTAAT GAAACCGAAA GCCCTAGAAC AAGAAGGGAG TGTAGTATTG
    CCATTTTTGA AGAAACTGCC AGAGACCAGC CAGGTCACTC TGCATAATTC TGATCTGCAA
    GAGCCACCCC GCAGGGTCAT GGTTCCGCTC AGGGTGCGGA ACGACTGGCG GCGCTCCCGG
    TTTTTCTCAC AGCCAGCTGA TTCTGGCAAT AGTGACAAAT CAAATCGTTT CAATTTCTGG
    TCCTGGGACC CAGAGGAAGA GCGGAAGCGG CAGGAGAGGT GGCAGCATGA GCAAGAACGC
    CTGCTTCAGG AGAAGTATCA GAAGGAGCAA GACAAACTGA AGGAGGAATG GGAAAAGGCA
    CAGAGAGAAG TTGAAGAGGA GGAGCGTAGA TACTATGAGG AGGAACGTAA GATAATCGAG
    GACACTGTAG TTCCATTCAT TGTTTCTTCA AACTCTGCAG AACTCCTCTC CTCTTCTTCC
    TCTACCACTG AAGGAAATAA AACAGCGAAC ACAAGTGATT CAAATGGCTC TCCAGAGGAA
    GGCAAACCAA AAGAAGTTAC AAGGCAGAAA AAGCTGGTTT GGGAGCAGGA CAGCATAGCA
    TCTCTGAAAA ACAAGGAAAA TGAACGCTGG CAAGAAAAGA GGAGCAGTGG AAATAAAGTT
    CTCTCAGGAC ACCCAGAGGT GGGTATCTTG AAAGGAGGTG ACCAGGAGTA CCAGGTGCCG
    GTGATGGAGC GCTGCTCGCC TGCCCGGTTG AATCTGCCAT TGACTCAGGA TGTCCCATGG
    AACCAGCAGC TGCTAACGAA GCAGTCCCCA CACCACGCAG AGGACGCCCG GCAGAGACCG
    CTGCTGGGGC AGAGTGCAGG ACAGCAGCCG AGCGCCGCAG GGGAAGGGAG GAGGGCAGGC
    TGTCATGAGA ACTTTCGATC TGGGCCATCG TCTCCCTGCT CACCTGCTGC ACCAAGTCAG
    TCACCCAACA GGTACAAAAG GTCTGTCAGC GGAAAGAAGC TTTGTTCCAC CTGTGGGCTT
    CCTCTTGGCA AGGGAGCTGC CATGATTATT GAAACGCTTG GTCTTTATTT CCATATTCAG
    TGTTTCAGGT GCGGCATTTG CAAGGGACAG CTGGGAGATG CAGCAAGTGG GACAGATGTC
    AGGATTAGAA ATGGTCTTCT GAACTGCAAT GATTGTTATA TCCGATCCAG AACTGCTGGA
    CAGCCAACTA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08412
    Clone ID Related Accession (Same CDS sequence) XM_004936081.2
    Accession Version XM_004936081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004936081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGCTG ATTCTGGCAA TAGTGACAAA
    TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG
    TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG
    AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG
    GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA
    GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT
    TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT
    TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG
    AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT
    GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA
    TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA
    GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA
    GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC
    TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG
    CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT
    GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT
    GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT
    ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004936138.1
    CDS156..5354
    Translation

    Target ORF information:

    RefSeq Version XM_004936081.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004936081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCAGCC CCGCGCTGGA GCTGGGAGAA GCGCAGCCCC CGCCCGACTC CCCGCAGCCC 
    GAGCCGGCCT TCGGCGAGGC GCAGAAGTGG ATCGAGCAAG TGACTGGCAG AAGTTTTGGT
    GAGAAGGACT TTCGTTCTGG ATTAGAAAAC GGCATTCTTC TGTGTGAGTT GCTGAATGCA
    ATAAAGCCAG GATTGGTCAA AAAGATCAAT AGACTACCAA CCCCCATTGC AGGTTTGGAT
    AATATCATCT TATTCTTAAG AGGCTGCAAG GAGCTGGGAC TTAAGGAATC TCAGCTGTTC
    GATCCTGGTG ACCTGCAGGA CACCTCAAAC AGAGTAACCG TCAAGAACGT TGACTGTAGT
    AGGAAGCTGA AAAATGTATT AGTCACCATT TATTGGCTAG GAAAAGCAGC AAACAGCTGC
    ACATCATATA GTGGATCAAC CCTGAACCTG AAGGAGTTTG AAGGTTTACT GGCACAAATG
    AGAAAGGAAA CAGAGGATAT CGAGAGCCCA AAGCGCAGCA TTCGGGACAG CGGCTACATA
    GACTGCTGGG ACTCGGAGCG CAGTGATTCC CTCTCCCCCC CGCGCCACGG CAGAGATGAT
    TCCTTTGACA GTCTGGACTC CTTTGGCTCC CGCTCGCAGC AGACACCGTC CCCAGATGTA
    GTGCTCAGGG GAAGCAGTGA TGGGAGAGGA AGTGACTCAG AAACTGACCT GCCACACAGA
    AAGATGCCAG ATGTGAAAAA AGATGATATG TTGGCGAGGC GGACCTCACA CGGTGAACCT
    AAATCAGCTG TGCCTTTTAA CCAGTACCTC CCAAACAAGA GCAATCAGAC TTTCTATGTA
    CCTGCTCCAC TTCGGAAAAA GAAAGCTGAA CGAGAAGAGT ATCGAAAGAG CTGGAGCACT
    GCGACTTCAC CGCTGGGAGA CAGACCTTTC AGCAAACCTG AAACCATAGA AGAAGAACAA
    AGTGAAACAT CAGGACTGTG TGAAAAGGAG AAAATGGGCT CCATGGCTCC TGAGGAAAGC
    CCTGTTAAAA TACAAGCAGA TTTAAATCAA AATGAAGGAG ATTCCATAGA CCAGTCTCCT
    TCATGTAGTG TAAAAGAAAC ATCATCACTC AAGGGAAAAG ATGAAGCAGA GATCAGAAAG
    TTAAGAAGAC TTGAACAAGC TGGCATTAAG GTCATGTCAG CTGCCCAGCG CTATGGCAGT
    AAAAAGCAAA CATGTGAAGA CAGAGGAAGC ACCCCAGACA TCATCCTTCG CAAGGAAAAC
    CCCTTCATGA CACGTTATCA AGGGAAGGAT TCTGGTTCAG AAGATGAAGC AGCAAGCAAA
    GTTCCTGATT TAGAAAAGGA TGACTTTGCC ACCCGGAGAG CCAGGATGAA TCAGCCCAAA
    CACGTAGTTC CATTTAACTC CTTTATAATT GGACCCTATA CGCAGAAAGA TAAAAGTAAA
    CTGGAAGACA ATAAAAAGCC ATTGAAGGTG GAAAAGCAGG AACATGCAAG AGGAAGCCAG
    GTGCACATGG CATCAGCAAG ACAAACAGTG ACATGCAAAG AGAACCCACT TCTGCAGACT
    GAGAAGAATA AGTCAGAGGA GGAAGAGCAG GTGAAAGTAG TTGATGCCGA GAGAGATGAT
    TTGGCTAAAC GGAAATCTCA GTGTGTCCTT CCTCAGCTAC AAGAAGATAG GATTTTTGTG
    AATGCCTCTA TAACAAAAGC AGATCTGGAG ACATGGAAAA GATTGAAACT GTCAGGAGAG
    CCCAGTGAAG AAGATTGCAA GTGTCCCAGT GACACTCTAC CAGCAGCTGA AGTCACAGTG
    CAAGCAGCAG TCCAGGATGA TTTTGCTGTC CGCAAAGCCA GAGCAAACAG AAAGGCTGGT
    AGCCTCAAGC AGAAGTTTGT GCATTTTGGG CCAGTAACTG AGATTGATCA GCAGAAGTGG
    GACAAGCTCA GCATCGGCAG AGCTTCACGC AGGTGTGAAG CAGAAGAGGA AAGCGGGAAA
    GACGGAGTGA AGGTTCACTC GGATGTTGAA AGCTCTCTTC CAACCACTGC TGGCAGTCTC
    CAGTTTGCTG AGCATGGTGA TGCAGTATCT AAGCCACAGA ATGAGCCCAG TGAGGTAGCG
    CAGTCAGATC CAGCTGAATG TTGCAGGAGC ATGCCGTCAC CTGCCGCTAC TATTGATGTC
    ATCTCTGAGA AAGAAGCCCT GTGCAGCTCG AACAATCAGC AAGAAAGCGC CGAAGAAGAA
    GAAAGCAGTG CAAATTTACC CGACATTGAG AGAGACGATA TGTTGGTTAG AAGGATGGGG
    ACCTTTCAGA GGCGCACAAC TAGCACTGTT CCAATCCACT GTCCTCCCCT GTCAGTAGCT
    CAGGAGAAGG ATTTGAAGAC TCCACGGGGA GCAGAGAGTA CTGTAACGCG AGAAGAGAGC
    CAGGAACAGC TTGAAATCCC TCATCAGCAG AGTGGCGGAA CAGAAAACAA AGTGAAGTTT
    CCAGACCCAC AAAGAGATGA CATGTTGGCC AGAAGAACAG GAGCTTTTCT AAAACAGCCG
    GGGCCAAGTG TTAAGCAGTT CCTTCCCATG CCATATTCAA AGCAGCAGAA TAAACAGGGC
    ACAAGTAGAG AACTTGAAAA GAAAACTGCA AGGCCTGAAA AATGCCCACA AGTCTGCATT
    ACTAGCTCTG GAAAACCTGT AATTCTACCT CAGCAAGAGC AGCAACAAGG GCAGGAAAGA
    TGCAGTCTCC CTCCTGCAGG ACAAGAGGAT ACTGTGTGCA CTGCCCAAGC GGGTGATCAT
    AGCCTTAACG AAGTCCACCT TGATCAAACA TCTAAGCTTC AGGTGGTTCC TGAGGAAAAC
    AGAACTGAAA CATTGTCATC CAAGGATGAG CATCTACAAA CATTCGGCCC CAGAACTGCT
    GTGTCTGATG ACGCAGAGAG CGTGAGCATG TTTGACATGA GGAGTGAGGA AGAAGCCATG
    GCTCAGCCTC ACAGCAAAGC TCGCCATGAA AAGCTGCAGA ATATACACAA CCAGCTGAAA
    GAGGACGAGA CCAGGTGGCA AGATGACCTG GCTCGGTGGA AGAGTCGTCG AAGGAGTGCT
    TCCCAGGATT TAATTAAAAA AGAAGCTGAG AGAAAGAAAA TGGAAAGATT ATTGTCTGGA
    GAGGGAACAA ATGAGCGCAG AAAAAGCATC AAAACCTACC GAGAAATTGT GGAAGAGAAA
    GAGAGAAGAG AACGGGAGCT TCATGAGGCA TACAAGAATG CAAAGACCCA GGAGGAGGCT
    GAGAGCATAC TCCAGCAGTA CATCGAAAGA TTCACCATTA GCGAAGCTGT CCTTGAGCGT
    TTGCAGATGC CAAAAATCCT GGAAAGAAGC CATTCAGTGG AGCCAAATTC CCCACTGAAA
    GACCCGAATC CTCTGAGATA CTTAAGGCAA CAATCACTGC CTGCTCCAAA ATTCACTGCC
    ACCATTGAAG CAACCATTGT TCCCACCACT GAATTAGAGC CCAGTGGTTC GACAGGCCGC
    ACATCTCCCA GCAAAAGTGT TGTCTCCAAG GCTGTGCCTA TGCTGACACC CAAGCCTTAC
    TCTCAACCCA AAAATACACA GGAAGTTTTA AAGAACTTTA AGGTAGATGG AAAAGTCAGC
    ATGAATGGAG AGAATTTCAA CGGAGTTGAA GAGAAGGATA AAGAGTGTAC AACAGTAATA
    TTTACTCAAT CGCCAAGCAG ATCTCTGAAA TTTGATGGAG TAGCCAGAGG GGACGGGCCC
    CCCATAGAGT TGAAGAAGGA CCCCACAAGT GTTGAGCTCA GTTTACAGAG GCCTGCCACT
    CAGAGTGTGC ACAGAGAGCC AACAGCCTTG CCGGGGGAAG CAGATCCGGC CGCCAGCCAG
    CAGGCCGAGG GCAGCCCTGG AGCAGTGGGA CACGGGAGCC CGGCAGACCT GTCCCCAGGT
    TCAGAACAAA AGCAATTCAA GTCAACTGTA CTTTGTGCCA GCCCTGTTGT GAAGAGCTTA
    GAATTTTCTC CTAGTCCTGT CCCTCCAGAG GAAACTGCTC CAAGTGAGAA AGATGTGAAG
    AAACCTGAAA TGGAGAAGCA GAAAGAGGCA GCACTCCCTG CTATGCAGGA GGTAATGAAA
    CCGAAAGCCC TAGAACAAGA AGGGAGTGTA GTATTGCCAT TTTTGAAGAA ACTGCCAGAG
    ACCAGCCAGG TCACTCTGCA TAATTCTGAT CTGCAAGCTG ATTCTGGCAA TAGTGACAAA
    TCAAATCGTT TCAATTTCTG GTCCTGGGAC CCAGAGGAAG AGCGGAAGCG GCAGGAGAGG
    TGGCAGCATG AGCAAGAACG CCTGCTTCAG GAGAAGTATC AGAAGGAGCA AGACAAACTG
    AAGGAGGAAT GGGAAAAGGC ACAGAGAGAA GTTGAAGAGG AGGAGCGTAG ATACTATGAG
    GAGGAACGTA AGATAATCGA GGACACTGTA GTTCCATTCA TTGTTTCTTC AAACTCTGCA
    GAACTCCTCT CCTCTTCTTC CTCTACCACT GAAGGAAATA AAACAGCGAA CACAAGTGAT
    TCAAATGGCT CTCCAGAGGA AGGCAAACCA AAAGAAGTTA CAAGGCAGAA AAAGCTGGTT
    TGGGAGCAGG ACAGCATAGC ATCTCTGAAA AACAAGGAAA ATGAACGCTG GCAAGAAAAG
    AGGAGCAGTG GAAATAAAGT TCTCTCAGGA CACCCAGAGG TGGGTATCTT GAAAGGAGGT
    GACCAGGAGT ACCAGGTGCC GGTGATGGAG CGCTGCTCGC CTGCCCGGTT GAATCTGCCA
    TTGACTCAGG ATGTCCCATG GAACCAGCAG CTGCTAACGA AGCAGTCCCC ACACCACGCA
    GAGGACGCCC GGCAGAGACC GCTGCTGGGG CAGAGTGCAG GACAGCAGCC GAGCGCCGCA
    GGGGAAGGGA GGAGGGCAGG CTGTCATGAG AACTTTCGAT CTGGGCCATC GTCTCCCTGC
    TCACCTGCTG CACCAAGTCA GTCACCCAAC AGGTACAAAA GGTCTGTCAG CGGAAAGAAG
    CTTTGTTCCA CCTGTGGGCT TCCTCTTGGC AAGGGAGCTG CCATGATTAT TGAAACGCTT
    GGTCTTTATT TCCATATTCA GTGTTTCAGG TGCGGCATTT GCAAGGGACA GCTGGGAGAT
    GCAGCAAGTG GGACAGATGT CAGGATTAGA AATGGTCTTC TGAACTGCAA TGATTGTTAT
    ATCCGATCCA GAACTGCTGG ACAGCCAACT ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.