LIMCH1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMCH1
Entrez Gene ID	461186
Full Name	LIM and calponin homology domains 1
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016951470.1	XP_016806959.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_016951473.1	XP_016806962.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_016951474.1	XP_016806963.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_016951476.1	XP_016806965.1	LIM and calponin homology domains-containing protein 1 isoform X11
XM_009447582.2	XP_009445857.2	LIM and calponin homology domains-containing protein 1 isoform X16
XM_009447588.2	XP_009445863.2	LIM and calponin homology domains-containing protein 1 isoform X22
XM_016951471.2	XP_016806960.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_009447572.3	XP_009445847.2	LIM and calponin homology domains-containing protein 1 isoform X6
XM_024356078.1	XP_024211846.1	LIM and calponin homology domains-containing protein 1 isoform X14
XM_009447587.3	XP_009445862.2	LIM and calponin homology domains-containing protein 1 isoform X22
XM_517164.6	XP_517164.3	LIM and calponin homology domains-containing protein 1 isoform X26
XM_024356082.1	XP_024211850.1	LIM and calponin homology domains-containing protein 1 isoform X28
XM_016951482.2	XP_016806971.1	LIM and calponin homology domains-containing protein 1 isoform X30
XM_009447564.2	XP_009445839.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_016951477.1	XP_016806966.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_016951478.1	XP_016806967.1	LIM and calponin homology domains-containing protein 1 isoform X15
XM_016951479.1	XP_016806968.1	LIM and calponin homology domains-containing protein 1 isoform X17
XM_009447586.2	XP_009445861.2	LIM and calponin homology domains-containing protein 1 isoform X20
XM_016951481.1	XP_016806970.1	LIM and calponin homology domains-containing protein 1 isoform X24
XM_517164.5	XP_517164.3	LIM and calponin homology domains-containing protein 1 isoform X27
XM_001148737.4	XP_001148737.1	LIM and calponin homology domains-containing protein 1 isoform X29
XM_009447563.2	XP_009445838.2	LIM and calponin homology domains-containing protein 1 isoform X1
XM_016951468.2	XP_016806957.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_016951469.2	XP_016806958.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_016951470.2	XP_016806959.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_016951472.2	XP_016806961.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_016951474.2	XP_016806963.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_009447564.3	XP_009445839.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_009447566.3	XP_009445841.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_009447588.3	XP_009445863.2	LIM and calponin homology domains-containing protein 1 isoform X23
XM_024356081.1	XP_024211849.1	LIM and calponin homology domains-containing protein 1 isoform X25
XM_016951475.1	XP_016806964.1	LIM and calponin homology domains-containing protein 1 isoform X10
XM_009447566.2	XP_009445841.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_009447577.2	XP_009445852.2	LIM and calponin homology domains-containing protein 1 isoform X14
XM_009447583.2	XP_009445858.2	LIM and calponin homology domains-containing protein 1 isoform X19
XM_009447579.2	XP_009445854.1	LIM and calponin homology domains-containing protein 1 isoform X25
XM_016951469.1	XP_016806958.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_009447563.3	XP_009445838.2	LIM and calponin homology domains-containing protein 1 isoform X1
XM_009447561.3	XP_009445836.1	LIM and calponin homology domains-containing protein 1 isoform X12
XM_009447567.3	XP_009445842.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_024356080.1	XP_024211848.1	LIM and calponin homology domains-containing protein 1 isoform X16
XM_016951479.2	XP_016806968.1	LIM and calponin homology domains-containing protein 1 isoform X18
XM_001148737.5	XP_001148737.1	LIM and calponin homology domains-containing protein 1 isoform X29
XM_016951468.1	XP_016806957.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_016951472.1	XP_016806961.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_009447578.2	XP_009445853.1	LIM and calponin homology domains-containing protein 1 isoform X23
XM_009447581.2	XP_009445856.1	LIM and calponin homology domains-containing protein 1 isoform X26
XM_001148461.4	XP_001148461.1	LIM and calponin homology domains-containing protein 1 isoform X31
XM_016951475.2	XP_016806964.1	LIM and calponin homology domains-containing protein 1 isoform X10
XM_016951477.2	XP_016806966.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_016951480.2	XP_016806969.1	LIM and calponin homology domains-containing protein 1 isoform X19
XM_009447583.3	XP_009445858.2	LIM and calponin homology domains-containing protein 1 isoform X20
XM_001148461.5	XP_001148461.1	LIM and calponin homology domains-containing protein 1 isoform X31
XM_016951471.1	XP_016806960.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_009447561.2	XP_009445836.1	LIM and calponin homology domains-containing protein 1 isoform X12
XM_016951480.1	XP_016806969.1	LIM and calponin homology domains-containing protein 1 isoform X18
XM_009447587.2	XP_009445862.2	LIM and calponin homology domains-containing protein 1 isoform X21
XM_009447584.2	XP_009445859.1	LIM and calponin homology domains-containing protein 1 isoform X28
XM_016951482.1	XP_016806971.1	LIM and calponin homology domains-containing protein 1 isoform X30
XM_009447572.2	XP_009445847.2	LIM and calponin homology domains-containing protein 1 isoform X6
XM_009447567.2	XP_009445842.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_016951473.2	XP_016806962.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_016951476.2	XP_016806965.1	LIM and calponin homology domains-containing protein 1 isoform X11
XM_024356079.1	XP_024211847.1	LIM and calponin homology domains-containing protein 1 isoform X15
XM_009447582.3	XP_009445857.2	LIM and calponin homology domains-containing protein 1 isoform X17
XM_009447586.3	XP_009445861.2	LIM and calponin homology domains-containing protein 1 isoform X21
XM_009447579.3	XP_009445854.1	LIM and calponin homology domains-containing protein 1 isoform X24
XM_009447584.3	XP_009445859.1	LIM and calponin homology domains-containing protein 1 isoform X27

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1
Homo sapiens (human)	LIMCH1	NP_055803.2

Gene Ontology

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc07913	XM_001148737.5 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc07914	XM_001148461.5 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc07915	XM_517164.6 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38754	XM_009447578.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38755	XM_009447579.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38757	XM_009447581.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38759	XM_009447584.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38761	XM_016951482.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc105780	XM_024356082.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc38756	XM_024356081.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc83659	XM_016951481.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc105777	XM_024356078.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc105778	XM_024356079.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc105779	XM_024356080.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc83649	XM_009447567.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83649	XM_009447566.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83649	XM_016951477.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83649	XM_009447564.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83650	XM_009447577.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83651	XM_016951478.1 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83652	XM_009447582.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83653	XM_016951479.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83654	XM_016951480.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83655	XM_009447583.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83656	XM_009447586.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83657	XM_009447587.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83658	XM_009447588.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83637	XM_009447563.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83638	XM_016951468.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83639	XM_016951469.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83640	XM_016951470.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83641	XM_016951471.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83642	XM_009447572.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83643	XM_016951472.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83644	XM_016951473.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83645	XM_016951474.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83646	XM_016951475.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83647	XM_016951476.2 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83648	XM_009447561.3 Latest version!	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OPc07915	XM_517164.5	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc83640	XM_016951470.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83645	XM_016951474.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83649	XM_009447564.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83658	XM_009447588.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83649	XM_009447566.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83639	XM_016951469.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83637	XM_009447563.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83653	XM_016951479.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc07913	XM_001148737.4	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc83638	XM_016951468.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83643	XM_016951472.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83646	XM_016951475.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83649	XM_016951477.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83655	XM_009447583.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc07914	XM_001148461.4	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc83641	XM_016951471.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83648	XM_009447561.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83654	XM_016951480.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83657	XM_009447587.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc38761	XM_016951482.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc83642	XM_009447572.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83649	XM_009447567.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc83644	XM_016951473.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83647	XM_016951476.1	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc83652	XM_009447582.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc83656	XM_009447586.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc38755	XM_009447579.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OPc38759	XM_009447584.2	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMCH1 ( XM_001148737.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148737.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.

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CloneID	OPc07913
Clone ID Related Accession (Same CDS sequence)	XM_001148737.4 , XM_001148737.5
Accession Version	XM_001148737.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001148737.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148737.1
CDS	300..3008
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_001148737.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148737.4

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_001148737.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148737.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OPc07913
Clone ID Related Accession (Same CDS sequence)	XM_001148737.4 , XM_001148737.5
Accession Version	XM_001148737.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001148737.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148737.1
CDS	373..3081
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_001148737.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148737.5

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_001148461.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148461.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OPc07914
Clone ID Related Accession (Same CDS sequence)	XM_001148461.4 , XM_001148461.5
Accession Version	XM_001148461.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001148461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148461.1
CDS	300..2972
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_001148461.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148461.4

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_001148461.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148461.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.

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CloneID	OPc07914
Clone ID Related Accession (Same CDS sequence)	XM_001148461.4 , XM_001148461.5
Accession Version	XM_001148461.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001148461.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148461.1
CDS	373..3045
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_001148461.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148461.5

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_517164.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_517164.3 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OPc07915
Clone ID Related Accession (Same CDS sequence)	XM_517164.6 , XM_517164.5
Accession Version	XM_517164.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_517164.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_517164.3
CDS	373..3123
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_517164.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_517164.6

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_517164.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_517164.3 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.

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CloneID	OPc07915
Clone ID Related Accession (Same CDS sequence)	XM_517164.6 , XM_517164.5
Accession Version	XM_517164.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_517164.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_517164.3
CDS	363..3113
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_517164.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_517164.5

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447578.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445853.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OPc38754
Clone ID Related Accession (Same CDS sequence)	XM_009447578.2
Accession Version	XM_009447578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TACAACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445853.1
CDS	358..3195
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447578.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447578.2

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TACAACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447579.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445854.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OPc38755
Clone ID Related Accession (Same CDS sequence)	XM_009447579.2 , XM_009447579.3
Accession Version	XM_009447579.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445854.1
CDS	358..3153
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447579.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447579.2

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447579.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445854.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OPc38755
Clone ID Related Accession (Same CDS sequence)	XM_009447579.2 , XM_009447579.3
Accession Version	XM_009447579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445854.1
CDS	368..3163
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447579.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447579.3

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447581.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445856.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OPc38757
Clone ID Related Accession (Same CDS sequence)	XM_009447581.2
Accession Version	XM_009447581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445856.1
CDS	52..2832
Translation	MTAVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447581.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447581.2

ORF Insert Sequence:

ATGACTGCAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447584.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445859.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OPc38759
Clone ID Related Accession (Same CDS sequence)	XM_009447584.2 , XM_009447584.3
Accession Version	XM_009447584.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGTTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTTGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGATGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGGGGACAAG AATGATGGTG GAAAACCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445859.1
CDS	578..3310
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447584.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447584.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGTTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTTGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGATGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGGGGACAAG AATGATGGTG GAAAACCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447584.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445859.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OPc38759
Clone ID Related Accession (Same CDS sequence)	XM_009447584.2 , XM_009447584.3
Accession Version	XM_009447584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGTTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTTGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGATGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGGGGACAAG AATGATGGTG GAAAACCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445859.1
CDS	578..3310
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447584.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447584.3

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGTTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTTGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGATGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGGGGACAAG AATGATGGTG GAAAACCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTACAA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951482.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806971.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OPc38761
Clone ID Related Accession (Same CDS sequence)	XM_016951482.2 , XM_016951482.1
Accession Version	XM_016951482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGTT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTTGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGATGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGGG 
GACAAGAATG ATGGTGGAAA ACCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTACA 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806971.1
CDS	404..3097
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951482.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951482.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGTT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTTGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGATGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGGG 
GACAAGAATG ATGGTGGAAA ACCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTACA 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951482.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806971.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OPc38761
Clone ID Related Accession (Same CDS sequence)	XM_016951482.2 , XM_016951482.1
Accession Version	XM_016951482.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGTT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTTGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGATGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGGG 
GACAAGAATG ATGGTGGAAA ACCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTACA 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806971.1
CDS	387..3080
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951482.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951482.1

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGTT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTTGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGATGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGGG 
GACAAGAATG ATGGTGGAAA ACCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTACA 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024356082.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211850.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OPc105780
Clone ID Related Accession (Same CDS sequence)	XM_024356082.1
Accession Version	XM_024356082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC 
AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA 
CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA 
ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA 
TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG 
GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA 
GTCCATGAAG ACCATCAGCT GGATGCCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA 
CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA 
GATGTGAAGC CAAAAACCCT CCCACTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC 
AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211850.1
CDS	373..3084
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024356082.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356082.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC 
AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA 
CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA 
ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA 
TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG 
GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA 
GTCCATGAAG ACCATCAGCT GGATGCCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA 
CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA 
GATGTGAAGC CAAAAACCCT CCCACTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC 
AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024356081.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211849.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OPc38756
Clone ID Related Accession (Same CDS sequence)	XM_024356081.1
Accession Version	XM_024356081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC 
TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT 
CACATCCAAT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGCGGG 
ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA 
AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211849.1
CDS	373..3159
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024356081.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356081.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGTTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTTG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGATG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGGGGACAA GAATGATGGT GGAAAACCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTACAACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC 
TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT 
CACATCCAAT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGCGGG 
ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA 
AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951481.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806970.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OPc83659
Clone ID Related Accession (Same CDS sequence)	XM_016951481.1
Accession Version	XM_016951481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCAG GAGTGAAACT GTCCTCTCTG GCTTTAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806970.1
CDS	16..2823
Translation	MTAGVKLSSLALVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951481.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951481.1

ORF Insert Sequence:

ATGACTGCAG GAGTGAAACT GTCCTCTCTG GCTTTAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024356078.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211846.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

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CloneID	OPc105777
Clone ID Related Accession (Same CDS sequence)	XM_024356078.1
Accession Version	XM_024356078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA CAACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAATGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGCGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211846.1
CDS	435..4838
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024356078.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356078.1

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA CAACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAATGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGCGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024356079.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211847.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OPc105778
Clone ID Related Accession (Same CDS sequence)	XM_024356079.1
Accession Version	XM_024356079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGAAACC AGGGCCACAC AGAAGAGGTG 
AAGTTGATAG TGACCTGTAA CATGAGGGCT CAGGAAAGTG AACCTGTGGA AGGAGGACTC 
AGGAAGGTGC CAGATCTTCA CAAGGATGAC CTGGCCCAGC GGAGAATTCA GGGCAGCCTT 
GCCCCTCACC GTGAGCCCCC GAGCTTCATT ACGCTCTCCA ACATAACAGA AGCTGACTTG 
GAGACGTGGG AGAGACTCAA AGTGTCAGAA AAGGCGAGGG ATGGAGATGT TCAGCACATC 
TGTGCTTCTG AGCCTTCCCC AGAAATTAAA GCAGAAACTG CCATTCGTGA TGACTTTGCC 
AACCGCAAAG CAAGGGCCTC TAAGAAAGCT TCCAGCCCCA GGCAAAAGTT TGTGCACTTT 
GGGCCAGTGA CGGAGATAGA TCAGCAGAAA TGGAAGCGGC TTAGCATTGG CAAGGCTGGG 
CCTAGAGAGG ATGAAGAAGA AGTCATCTGT CATGGCAGCA AGATTCAAAC GGACTCTGTG 
TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA AAGGGCCACC AAATATTTAA TCGACAAAAT 
GACTGCAGAA CAATGAATTC TGGCCGAGGT GACTATTGCA GAAGGGCCTC GTGGCTGGCT 
CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC TGCAGTTTGG GCGAGTGCCC GAGGGGGACA 
GAGGAAGTTA CATCCAGACA GCTGCCACAG GATGGCAAAG AAGAAATGGA AAGCGCTCCA 
AGAGATTCTG AGAGGCTGTC AAAGGCAGAA AGATCAGAGG ACAGCAGCCA GCCACTGCTG 
TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA GGGACAGAAG AGAAGTTGGA GAAGATGACA 
GCTCCTGCCT GGAGTGGCAG TGGACTGAAA GGCCAGCGAA AGTTGGATGA TTCACGAAAA 
GATGACATGA TGGCCAGGAG AACTGGGATG TCCCTTAGAC ACACTGGATC CAACCCAAAC 
CAGTTCCTTC CAGTTCCATT TGCAAAGCAA CAGGATGTGG AAGAATCTTC TAAAGGTCTA 
CCCATGAAAG ATCAGAGATA CGGTCCGAGA ACTCCTGTGT CTGATGACGC AGAGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TACAACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211847.1
CDS	70..4467
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024356079.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356079.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGAAACC AGGGCCACAC AGAAGAGGTG 
AAGTTGATAG TGACCTGTAA CATGAGGGCT CAGGAAAGTG AACCTGTGGA AGGAGGACTC 
AGGAAGGTGC CAGATCTTCA CAAGGATGAC CTGGCCCAGC GGAGAATTCA GGGCAGCCTT 
GCCCCTCACC GTGAGCCCCC GAGCTTCATT ACGCTCTCCA ACATAACAGA AGCTGACTTG 
GAGACGTGGG AGAGACTCAA AGTGTCAGAA AAGGCGAGGG ATGGAGATGT TCAGCACATC 
TGTGCTTCTG AGCCTTCCCC AGAAATTAAA GCAGAAACTG CCATTCGTGA TGACTTTGCC 
AACCGCAAAG CAAGGGCCTC TAAGAAAGCT TCCAGCCCCA GGCAAAAGTT TGTGCACTTT 
GGGCCAGTGA CGGAGATAGA TCAGCAGAAA TGGAAGCGGC TTAGCATTGG CAAGGCTGGG 
CCTAGAGAGG ATGAAGAAGA AGTCATCTGT CATGGCAGCA AGATTCAAAC GGACTCTGTG 
TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA AAGGGCCACC AAATATTTAA TCGACAAAAT 
GACTGCAGAA CAATGAATTC TGGCCGAGGT GACTATTGCA GAAGGGCCTC GTGGCTGGCT 
CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC TGCAGTTTGG GCGAGTGCCC GAGGGGGACA 
GAGGAAGTTA CATCCAGACA GCTGCCACAG GATGGCAAAG AAGAAATGGA AAGCGCTCCA 
AGAGATTCTG AGAGGCTGTC AAAGGCAGAA AGATCAGAGG ACAGCAGCCA GCCACTGCTG 
TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA GGGACAGAAG AGAAGTTGGA GAAGATGACA 
GCTCCTGCCT GGAGTGGCAG TGGACTGAAA GGCCAGCGAA AGTTGGATGA TTCACGAAAA 
GATGACATGA TGGCCAGGAG AACTGGGATG TCCCTTAGAC ACACTGGATC CAACCCAAAC 
CAGTTCCTTC CAGTTCCATT TGCAAAGCAA CAGGATGTGG AAGAATCTTC TAAAGGTCTA 
CCCATGAAAG ATCAGAGATA CGGTCCGAGA ACTCCTGTGT CTGATGACGC AGAGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTTGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GATGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGGGG ACAAGAATGA TGGTGGAAAA 
CCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TACAACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024356080.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211848.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OPc105779
Clone ID Related Accession (Same CDS sequence)	XM_024356080.1
Accession Version	XM_024356080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211848.1
CDS	65..3322
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024356080.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356080.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAA 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGCGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447564.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445839.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

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CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447564.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445839.1
CDS	566..5056
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447564.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447564.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951477.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806966.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

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CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_016951477.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806966.1
CDS	411..4901
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951477.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951477.1

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447564.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445839.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445839.1
CDS	566..5056
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447564.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447564.3

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447566.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445841.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445841.1
CDS	550..5040
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447566.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447566.3

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447566.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445841.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

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CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447566.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445841.1
CDS	554..5044
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447566.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447566.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447567.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445842.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

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CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447567.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445842.1
CDS	444..4934
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447567.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447567.3

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951477.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806966.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_016951477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806966.1
CDS	413..4903
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951477.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951477.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447567.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445842.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPc83649
Clone ID Related Accession (Same CDS sequence)	XM_009447564.2 , XM_016951477.1 , XM_009447564.3 , XM_009447566.3 , XM_009447566.2 , XM_009447567.3 , XM_016951477.2 , XM_009447567.2
Accession Version	XM_009447567.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445842.1
CDS	442..4932
Translation	MRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447567.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447567.2

ORF Insert Sequence:

ATGCGAAAGG AGACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CGGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGCGCGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG TTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCGGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGAT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AACGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTCTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAG ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAT GGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG TTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTTGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGATGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG GGGACAAGAA TGATGGTGGA AAACCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTACAACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATGCCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCACTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447577.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445852.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

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CloneID	OPc83650
Clone ID Related Accession (Same CDS sequence)	XM_009447577.2
Accession Version	XM_009447577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG 
AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA CAACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445852.2
CDS	68..3403
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447577.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447577.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG 
AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA CAACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TGCCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC ACTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAATG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGCGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951478.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806967.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OPc83651
Clone ID Related Accession (Same CDS sequence)	XM_016951478.1
Accession Version	XM_016951478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTCAGTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG 
AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT 
GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AATGTTTCAG GTGTGGAATT 
TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC GGGACGGATG TTAGGATTCG AAATGGTCTC 
CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806967.1
CDS	68..3367
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951478.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951478.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTCAGTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATGCCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCACTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCTTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG 
AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT 
GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AATGTTTCAG GTGTGGAATT 
TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC GGGACGGATG TTAGGATTCG AAATGGTCTC 
CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447582.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445857.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OPc83652
Clone ID Related Accession (Same CDS sequence)	XM_009447582.2 , XM_009447582.3
Accession Version	XM_009447582.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445857.2
CDS	51..3302
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447582.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447582.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447582.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445857.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

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CloneID	OPc83652
Clone ID Related Accession (Same CDS sequence)	XM_009447582.2 , XM_009447582.3
Accession Version	XM_009447582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445857.2
CDS	66..3317
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447582.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447582.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAATGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GCGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951479.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806968.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OPc83653
Clone ID Related Accession (Same CDS sequence)	XM_016951479.1 , XM_016951479.2
Accession Version	XM_016951479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806968.1
CDS	67..3315
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951479.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951479.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951479.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806968.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OPc83653
Clone ID Related Accession (Same CDS sequence)	XM_016951479.1 , XM_016951479.2
Accession Version	XM_016951479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806968.1
CDS	65..3313
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951479.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951479.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCTTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA ATGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGCG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951480.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806969.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OPc83654
Clone ID Related Accession (Same CDS sequence)	XM_016951480.2 , XM_016951480.1
Accession Version	XM_016951480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCT 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806969.1
CDS	65..3277
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951480.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951480.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCT 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951480.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806969.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OPc83654
Clone ID Related Accession (Same CDS sequence)	XM_016951480.2 , XM_016951480.1
Accession Version	XM_016951480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCT 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806969.1
CDS	67..3279
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951480.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951480.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCT 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447583.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445858.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OPc83655
Clone ID Related Accession (Same CDS sequence)	XM_009447583.2 , XM_009447583.3
Accession Version	XM_009447583.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATGCCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CACTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445858.2
CDS	51..3260
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447583.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447583.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATGCCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CACTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447583.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445858.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OPc83655
Clone ID Related Accession (Same CDS sequence)	XM_009447583.2 , XM_009447583.3
Accession Version	XM_009447583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATGCCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CACTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445858.2
CDS	66..3275
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447583.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447583.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTACAACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATGCCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CACTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447586.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445861.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

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CloneID	OPc83656
Clone ID Related Accession (Same CDS sequence)	XM_009447586.2 , XM_009447586.3
Accession Version	XM_009447586.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445861.2
CDS	51..3224
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447586.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447586.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447586.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445861.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OPc83656
Clone ID Related Accession (Same CDS sequence)	XM_009447586.2 , XM_009447586.3
Accession Version	XM_009447586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445861.2
CDS	51..3224
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447586.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447586.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAATGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGCGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447587.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445862.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OPc83657
Clone ID Related Accession (Same CDS sequence)	XM_009447587.3 , XM_009447587.2
Accession Version	XM_009447587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445862.2
CDS	51..3221
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447587.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447587.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447587.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445862.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OPc83657
Clone ID Related Accession (Same CDS sequence)	XM_009447587.3 , XM_009447587.2
Accession Version	XM_009447587.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445862.2
CDS	51..3221
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447587.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447587.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGTTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT TGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA TGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGGGGAC 
AAGAATGATG GTGGAAAACC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATGCCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCACTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAATGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG CGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447588.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445863.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OPc83658
Clone ID Related Accession (Same CDS sequence)	XM_009447588.2 , XM_009447588.3
Accession Version	XM_009447588.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAATGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGCGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445863.2
CDS	67..3201
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447588.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447588.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAATGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGCGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447588.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445863.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OPc83658
Clone ID Related Accession (Same CDS sequence)	XM_009447588.2 , XM_009447588.3
Accession Version	XM_009447588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAATGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGCGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445863.2
CDS	65..3199
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447588.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447588.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGT TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTTGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGATGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGG GGACAAGAAT GATGGTGGAA AACCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATGCC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCACTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAATGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGCGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447563.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445838.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc83637
Clone ID Related Accession (Same CDS sequence)	XM_009447563.2 , XM_009447563.3
Accession Version	XM_009447563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445838.2
CDS	73..5040
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447563.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447563.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447563.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445838.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

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CloneID	OPc83637
Clone ID Related Accession (Same CDS sequence)	XM_009447563.2 , XM_009447563.3
Accession Version	XM_009447563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445838.2
CDS	72..5039
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447563.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447563.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAA TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGCGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951468.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806957.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OPc83638
Clone ID Related Accession (Same CDS sequence)	XM_016951468.2 , XM_016951468.1
Accession Version	XM_016951468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806957.1
CDS	72..5036
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951468.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951468.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951468.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806957.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OPc83638
Clone ID Related Accession (Same CDS sequence)	XM_016951468.2 , XM_016951468.1
Accession Version	XM_016951468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806957.1
CDS	73..5037
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951468.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951468.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951469.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806958.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

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CloneID	OPc83639
Clone ID Related Accession (Same CDS sequence)	XM_016951469.2 , XM_016951469.1
Accession Version	XM_016951469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA ATGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGCG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806958.1
CDS	72..5030
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951469.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951469.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA ATGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGCG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951469.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806958.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

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CloneID	OPc83639
Clone ID Related Accession (Same CDS sequence)	XM_016951469.2 , XM_016951469.1
Accession Version	XM_016951469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA ATGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGCG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806958.1
CDS	73..5031
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951469.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951469.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCTTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA ATGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGCG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951470.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806959.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc83640
Clone ID Related Accession (Same CDS sequence)	XM_016951470.1 , XM_016951470.2
Accession Version	XM_016951470.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806959.1
CDS	72..4964
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951470.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951470.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951470.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806959.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

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CloneID	OPc83640
Clone ID Related Accession (Same CDS sequence)	XM_016951470.1 , XM_016951470.2
Accession Version	XM_016951470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806959.1
CDS	71..4963
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951470.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951470.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATGC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCACT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC TTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAATGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGCGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951471.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806960.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OPc83641
Clone ID Related Accession (Same CDS sequence)	XM_016951471.2 , XM_016951471.1
Accession Version	XM_016951471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806960.1
CDS	71..4960
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951471.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951471.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951471.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806960.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OPc83641
Clone ID Related Accession (Same CDS sequence)	XM_016951471.2 , XM_016951471.1
Accession Version	XM_016951471.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806960.1
CDS	72..4961
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951471.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951471.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447572.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445847.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OPc83642
Clone ID Related Accession (Same CDS sequence)	XM_009447572.3 , XM_009447572.2
Accession Version	XM_009447572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445847.2
CDS	71..4951
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447572.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447572.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447572.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445847.2 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OPc83642
Clone ID Related Accession (Same CDS sequence)	XM_009447572.3 , XM_009447572.2
Accession Version	XM_009447572.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445847.2
CDS	72..4952
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447572.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447572.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951472.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806961.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPc83643
Clone ID Related Accession (Same CDS sequence)	XM_016951472.2 , XM_016951472.1
Accession Version	XM_016951472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806961.1
CDS	71..4951
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951472.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951472.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951472.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806961.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

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CloneID	OPc83643
Clone ID Related Accession (Same CDS sequence)	XM_016951472.2 , XM_016951472.1
Accession Version	XM_016951472.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806961.1
CDS	72..4952
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951472.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951472.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATGCCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCACTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951473.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806962.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

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CloneID	OPc83644
Clone ID Related Accession (Same CDS sequence)	XM_016951473.1 , XM_016951473.2
Accession Version	XM_016951473.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAATGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGCGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806962.1
CDS	72..4874
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951473.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951473.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAATGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGCGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951473.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806962.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

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CloneID	OPc83644
Clone ID Related Accession (Same CDS sequence)	XM_016951473.1 , XM_016951473.2
Accession Version	XM_016951473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAATGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGCGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806962.1
CDS	71..4873
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951473.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951473.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTACAA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAATGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGCGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951474.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806963.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

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CloneID	OPc83645
Clone ID Related Accession (Same CDS sequence)	XM_016951474.1 , XM_016951474.2
Accession Version	XM_016951474.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806963.1
CDS	74..4843
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951474.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951474.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951474.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806963.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPc83645
Clone ID Related Accession (Same CDS sequence)	XM_016951474.1 , XM_016951474.2
Accession Version	XM_016951474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806963.1
CDS	73..4842
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951474.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951474.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC GGAGAATTCA GGGCAGCCTT GCCCCTCACC GTGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGATAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAC GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTC TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAGACA GCTGCCACAG 
GATGGCAAAG AAGAAATGGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGCTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGTTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTTGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GATGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGGGG ACAAGAATGA TGGTGGAAAA CCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TACAACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATGCCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCACTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCTTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AATGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGC 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951475.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806964.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc83646
Clone ID Related Accession (Same CDS sequence)	XM_016951475.1 , XM_016951475.2
Accession Version	XM_016951475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGATAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAACGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTCTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAGACAGCT GCCACAGGAT 
GGCAAAGAAG AAATGGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGCTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGTTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTT 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAT 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGGGGACA AGAATGATGG TGGAAAACCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806964.1
CDS	72..4796
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951475.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951475.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGATAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAACGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTCTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAGACAGCT GCCACAGGAT 
GGCAAAGAAG AAATGGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGCTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGTTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTT 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAT 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGGGGACA AGAATGATGG TGGAAAACCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951475.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806964.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

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CloneID	OPc83646
Clone ID Related Accession (Same CDS sequence)	XM_016951475.1 , XM_016951475.2
Accession Version	XM_016951475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGATAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAACGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTCTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAGACAGCT GCCACAGGAT 
GGCAAAGAAG AAATGGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGCTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGTTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTT 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAT 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGGGGACA AGAATGATGG TGGAAAACCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806964.1
CDS	71..4795
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951475.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951475.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGTTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGATAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAACGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTCTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAGACAGCT GCCACAGGAT 
GGCAAAGAAG AAATGGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGCTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGTTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTT 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAT 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGGGGACA AGAATGATGG TGGAAAACCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAC AACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT GCCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCA CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCTTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAATGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGCGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951476.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806965.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

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CloneID	OPc83647
Clone ID Related Accession (Same CDS sequence)	XM_016951476.1 , XM_016951476.2
Accession Version	XM_016951476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGTCAAA AACAATTAAG TGAAGAGAAA 
GAAGCTATTC GTGATATTGT CCTTCGCAAA GAAAACTCTT TCCTGACCCA CCAACATGGC 
AACGATTCAG AGGCAGAAGG TGAAGTTGTG TGTCGACTGC CTGATCTTGA GAAGGATGAC 
TTTGCTGCAA GGAGAGCAAG GATGAACCAA ACCAAGCCAA TGGTGCCATT AAATCAACTC 
CTCTATGGCC CTTATCCAAA GAAAGGGGCA GAGAAATCAG ATGGCAGCAA ACAGCTCTCA 
AAGGGAATCT CAAAAAAAAG AAGTCTAGAA TATAAAAGAA ACCAGGGCCA CACAGAAGAG 
GTGAAGTTGA TAGTGACCTG TAACATGAGG GCTCAGGAAA GTGAACCTGT GGAAGGAGGA 
CTCAGGAAGG TGCCAGATCT TCACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGGGCAGC 
CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC ATTACGCTCT CCAACATAAC AGAAGCTGAC 
TTGGAGACGT GGGAGAGACT CAAAGTGTCA GAAAAGGCGA GGGATGGAGA TGTTCAGCAC 
ATCTGTGCTT CTGAGCCTTC CCCAGAAATT AAAGCAGAAA CTGCCATTCG TGATGACTTT 
GCCAACCGCA AAGCAAGGGC CTCTAAGAAA GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC 
TTTGGGCCAG TGACGGAGAT AGATCAGCAG AAATGGAAGC GGCTTAGCAT TGGCAAGGCT 
GGGCCTAGAG AGGATGAAGA AGAAGTCATC TGTCATGGCA GCAAGATTCA AACGGACTCT 
GTGTCTCCTG TCTCAGCGGC CACTTCCAGC TTAAAGGGCC ACCAAATATT TAATCGACAA 
AATGACTGCA GAACAATGAA TTCTGGCCGA GGTGACTATT GCAGAAGGGC CTCGTGGCTG 
GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG 
ACAGAGGAAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806965.1
CDS	72..4772
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951476.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951476.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGTCAAA AACAATTAAG TGAAGAGAAA 
GAAGCTATTC GTGATATTGT CCTTCGCAAA GAAAACTCTT TCCTGACCCA CCAACATGGC 
AACGATTCAG AGGCAGAAGG TGAAGTTGTG TGTCGACTGC CTGATCTTGA GAAGGATGAC 
TTTGCTGCAA GGAGAGCAAG GATGAACCAA ACCAAGCCAA TGGTGCCATT AAATCAACTC 
CTCTATGGCC CTTATCCAAA GAAAGGGGCA GAGAAATCAG ATGGCAGCAA ACAGCTCTCA 
AAGGGAATCT CAAAAAAAAG AAGTCTAGAA TATAAAAGAA ACCAGGGCCA CACAGAAGAG 
GTGAAGTTGA TAGTGACCTG TAACATGAGG GCTCAGGAAA GTGAACCTGT GGAAGGAGGA 
CTCAGGAAGG TGCCAGATCT TCACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGGGCAGC 
CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC ATTACGCTCT CCAACATAAC AGAAGCTGAC 
TTGGAGACGT GGGAGAGACT CAAAGTGTCA GAAAAGGCGA GGGATGGAGA TGTTCAGCAC 
ATCTGTGCTT CTGAGCCTTC CCCAGAAATT AAAGCAGAAA CTGCCATTCG TGATGACTTT 
GCCAACCGCA AAGCAAGGGC CTCTAAGAAA GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC 
TTTGGGCCAG TGACGGAGAT AGATCAGCAG AAATGGAAGC GGCTTAGCAT TGGCAAGGCT 
GGGCCTAGAG AGGATGAAGA AGAAGTCATC TGTCATGGCA GCAAGATTCA AACGGACTCT 
GTGTCTCCTG TCTCAGCGGC CACTTCCAGC TTAAAGGGCC ACCAAATATT TAATCGACAA 
AATGACTGCA GAACAATGAA TTCTGGCCGA GGTGACTATT GCAGAAGGGC CTCGTGGCTG 
GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG 
ACAGAGGAAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_016951476.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806965.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

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CloneID	OPc83647
Clone ID Related Accession (Same CDS sequence)	XM_016951476.1 , XM_016951476.2
Accession Version	XM_016951476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016951476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGTCAAA AACAATTAAG TGAAGAGAAA 
GAAGCTATTC GTGATATTGT CCTTCGCAAA GAAAACTCTT TCCTGACCCA CCAACATGGC 
AACGATTCAG AGGCAGAAGG TGAAGTTGTG TGTCGACTGC CTGATCTTGA GAAGGATGAC 
TTTGCTGCAA GGAGAGCAAG GATGAACCAA ACCAAGCCAA TGGTGCCATT AAATCAACTC 
CTCTATGGCC CTTATCCAAA GAAAGGGGCA GAGAAATCAG ATGGCAGCAA ACAGCTCTCA 
AAGGGAATCT CAAAAAAAAG AAGTCTAGAA TATAAAAGAA ACCAGGGCCA CACAGAAGAG 
GTGAAGTTGA TAGTGACCTG TAACATGAGG GCTCAGGAAA GTGAACCTGT GGAAGGAGGA 
CTCAGGAAGG TGCCAGATCT TCACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGGGCAGC 
CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC ATTACGCTCT CCAACATAAC AGAAGCTGAC 
TTGGAGACGT GGGAGAGACT CAAAGTGTCA GAAAAGGCGA GGGATGGAGA TGTTCAGCAC 
ATCTGTGCTT CTGAGCCTTC CCCAGAAATT AAAGCAGAAA CTGCCATTCG TGATGACTTT 
GCCAACCGCA AAGCAAGGGC CTCTAAGAAA GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC 
TTTGGGCCAG TGACGGAGAT AGATCAGCAG AAATGGAAGC GGCTTAGCAT TGGCAAGGCT 
GGGCCTAGAG AGGATGAAGA AGAAGTCATC TGTCATGGCA GCAAGATTCA AACGGACTCT 
GTGTCTCCTG TCTCAGCGGC CACTTCCAGC TTAAAGGGCC ACCAAATATT TAATCGACAA 
AATGACTGCA GAACAATGAA TTCTGGCCGA GGTGACTATT GCAGAAGGGC CTCGTGGCTG 
GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG 
ACAGAGGAAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806965.1
CDS	71..4771
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_016951476.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016951476.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TTTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGAGA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCGG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG CGCGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGTCAAA AACAATTAAG TGAAGAGAAA 
GAAGCTATTC GTGATATTGT CCTTCGCAAA GAAAACTCTT TCCTGACCCA CCAACATGGC 
AACGATTCAG AGGCAGAAGG TGAAGTTGTG TGTCGACTGC CTGATCTTGA GAAGGATGAC 
TTTGCTGCAA GGAGAGCAAG GATGAACCAA ACCAAGCCAA TGGTGCCATT AAATCAACTC 
CTCTATGGCC CTTATCCAAA GAAAGGGGCA GAGAAATCAG ATGGCAGCAA ACAGCTCTCA 
AAGGGAATCT CAAAAAAAAG AAGTCTAGAA TATAAAAGAA ACCAGGGCCA CACAGAAGAG 
GTGAAGTTGA TAGTGACCTG TAACATGAGG GCTCAGGAAA GTGAACCTGT GGAAGGAGGA 
CTCAGGAAGG TGCCAGATCT TCACAAGGAT GACCTGGCCC AGCGGAGAAT TCAGGGCAGC 
CTTGCCCCTC ACCGTGAGCC CCCGAGCTTC ATTACGCTCT CCAACATAAC AGAAGCTGAC 
TTGGAGACGT GGGAGAGACT CAAAGTGTCA GAAAAGGCGA GGGATGGAGA TGTTCAGCAC 
ATCTGTGCTT CTGAGCCTTC CCCAGAAATT AAAGCAGAAA CTGCCATTCG TGATGACTTT 
GCCAACCGCA AAGCAAGGGC CTCTAAGAAA GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC 
TTTGGGCCAG TGACGGAGAT AGATCAGCAG AAATGGAAGC GGCTTAGCAT TGGCAAGGCT 
GGGCCTAGAG AGGATGAAGA AGAAGTCATC TGTCATGGCA GCAAGATTCA AACGGACTCT 
GTGTCTCCTG TCTCAGCGGC CACTTCCAGC TTAAAGGGCC ACCAAATATT TAATCGACAA 
AATGACTGCA GAACAATGAA TTCTGGCCGA GGTGACTATT GCAGAAGGGC CTCGTGGCTG 
GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG 
ACAGAGGAAG TTACATCCAG ACAGCTGCCA CAGGATGGCA AAGAAGAAAT GGAAAGCGCT 
CCAAGAGATT CTGAGAGGCT GTCAAAGGCA GAAAGATCAG AGGACAGCAG CCAGCCACTG 
CTGTGTCCTC TGGCCTCTGA GTGTGAGGCT TCAGGGACAG AAGAGAAGTT GGAGAAGATG 
ACAGCTCCTG CCTGGAGTGG CAGTGGACTG AAAGGCCAGC GAAAGTTGGA TGATTCACGA 
AAAGATGACA TGATGGCCAG GAGAACTGGG ATGTCCCTTA GACACACTGG ATCCAACCCA 
AACCAGTTCC TTCCAGTTCC ATTTGCAAAG CAACAGGATG TGGAAGAATC TTCTAAAGGT 
CTACCCATGA AAGATCAGAG ATACGGTCCG AGAACTCCTG TGTCTGATGA CGCAGAGAGC 
ACCAGCATGT TTGACATGCG GTGTGAGGAG GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC 
CGCCAGGAGC AGCTGCAGCT GATAAATAAC CAGCTGAGGG AAGAGGACGA CAAATGGCAA 
GATGACCTGG CTCGTTGGAA GAGTCGTAGA AGAAGTGTTT CTCAGGACTT AATCAAGAAA 
GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA CTGGCTGGAG AAGATGGGAC AAGTGAACGA 
AGGAAAAGCA TCAAAACCTA CAGAGAAATT GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG 
TTGCATGAAG CATATAAGAA CGCTCGGTCC CAGGAGGAGG CAGAGGGGAT CCTTCAACAG 
TACATTGAGA GGTTCACCAT CAGTGAGGCT GTTCTTGAAC GCTTGGAGAT GCCAAAAATT 
CTGGAAAGAA GCCATTCAAC AGAGCCAAAT TTATCCTCCT TCCTGAATGA CCCCAATCCC 
ATGAAATACC TGCGGCAACA GTCACTGCCT CCACCCAAAT TCACTGCCAC TGTTGAAACC 
ACCATTGCTC GTGCCAGTGT TCTGGATACC AGCATGTCAG CAGGCAGTGG GTCTCCAAGC 
AAAACTGTCA CTCCCAAAGC AGTGCCTATG CTGACACCCA AGCCTTACTC CCAGCCCAAA 
AATTCTCAAG ATGTTCTGAA GACCTTTAAG GTAGATGGGA AAGTCAGTGT GAATGGAGAG 
ACGGTTCATA GAGAGGAGGA GAAGGAAAGA GAGTGTCCCA CGGTGGCACC TGCCCACTCC 
TTAACCAAAT CCCAGATGTT TGAAGGTGTG GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG 
AAACAAGACA ACGGTAGCAT CGAGATCAAC ATAAAGAAGC CAAACTCTGT TCCCCAAGAG 
CTCGCAGCAA CCACTGAGAA AACGGAACCG AATAGTCAAG GGGACAAGAA TGATGGTGGA 
AAACCAAGAA AAGGGAATAT AGAACTTGCC TCATCAGAAC CACAGCATTT TACAACAACT 
GTGACTCGAT GCAGCCCGAC CGTGGCCTTT GTGGAATTTC CCTCCAGCCC CCAGCTGAAG 
AATGATGTGT CGGAAGAAAA AGACCAGAAG AAACCAGAAA ATGAAATGAG TGGAAAGGTG 
GAGTTGGTGC TGTCACAAAA GGTGGTAAAG CCAAAATCTC CAGAACCCGA AGCAACGCTG 
ACATTTCCAT TTCTGGACAA AATGCCTGAA GCCAACCAAC TACATTTGCC AAATCTCAAT 
TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT CCGCTTAGAG TTCAGAACTC TTGGCGACGA 
TCCCAGTTTT TCTCCCAGTC AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTACAACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATGCCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCACTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCTT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAATGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG CGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447561.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445836.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

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CloneID	OPc83648
Clone ID Related Accession (Same CDS sequence)	XM_009447561.3 , XM_009447561.2
Accession Version	XM_009447561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009447561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGTTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCGG AGAATTCAGG GCAGCCTTGC CCCTCACCGT 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGATAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAACGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTCTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAGACAGC TGCCACAGGA TGGCAAAGAA GAAATGGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGCTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGTTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT TGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGGGGAC AAGAATGATG GTGGAAAACC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA CAACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TGCCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC ACTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAATG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGCGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445836.1
CDS	368..4873
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447561.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447561.3

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGTTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCGG AGAATTCAGG GCAGCCTTGC CCCTCACCGT 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGATAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAACGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTCTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAGACAGC TGCCACAGGA TGGCAAAGAA GAAATGGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGCTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGTTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT TGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGGGGAC AAGAATGATG GTGGAAAACC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA CAACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TGCCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC ACTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAATG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGCGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009447561.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445836.1 Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OPc83648
Clone ID Related Accession (Same CDS sequence)	XM_009447561.3 , XM_009447561.2
Accession Version	XM_009447561.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	LIM and calponin homology domains-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009447561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGTTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCGG AGAATTCAGG GCAGCCTTGC CCCTCACCGT 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGATAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAACGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTCTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAGACAGC TGCCACAGGA TGGCAAAGAA GAAATGGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGCTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGTTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT TGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGGGGAC AAGAATGATG GTGGAAAACC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA CAACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TGCCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC ACTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAATG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGCGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445836.1
CDS	358..4863
Translation	MDSERQVKETDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPVGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQRRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTEIDQQKWKRLSIGKAGPREDEEEVICHGSKIQTDSVSPVSAATSSLKGHQIFNRQNDCRTMNSGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSRQLPQDGKEEMESAPRDSERLSKAERSEDSSQPLLCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQGDKNDGGKPRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVTTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDAEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_009447561.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009447561.2

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGAGACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCGGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGCG CGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGTTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCGG AGAATTCAGG GCAGCCTTGC CCCTCACCGT 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGATAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAACGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTCTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAGACAGC TGCCACAGGA TGGCAAAGAA GAAATGGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGCTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGTTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT TGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA TGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGGGGAC AAGAATGATG GTGGAAAACC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA CAACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TGCCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC ACTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCTTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAATG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGCGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

LIMCH1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: