LIMCH1 cDNA ORF clone, Homo sapiens(human)

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06760 NM_014988.5
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$544.50-$762.30
$1089.00
OHu06341 NM_001112719.3
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $636.30
$909.00
OHu06342 NM_001112720.3
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $629.30
$899.00
OHu06603 NM_001289124.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65265 NM_001330983.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65266 NM_001330674.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65264 XM_011513655.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65267 XM_011513658.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu100276 NM_001330784.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100278 NM_001330982.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100281 NM_001330793.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100284 NM_001330786.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu105497 XM_024453938.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu94982 XM_017007903.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100274 NM_001330789.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu100280 NM_001330788.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu100283 NM_001330787.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu105496 XM_024453937.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94980 XM_017007898.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94981 XM_017007900.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu06336 NM_001112717.4
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu06340 NM_001112718.4
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $741.30
$1059.00
OHu06600 NM_001289122.3
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38534 NM_001330790.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38536 NM_001330791.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38537 NM_001330792.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu65262 XM_011513653.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu65263 XM_011513654.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu94976 XM_017007894.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94977 XM_017007895.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94978 XM_017007896.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94979 XM_017007897.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu38528 NM_001330672.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu38518 XM_005248057.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu38519 XM_005248058.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu38521 XM_005248060.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu38522 XM_005248061.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu38524 XM_006713996.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65253 XM_011513642.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65254 XM_011513643.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65255 XM_011513644.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65256 XM_011513645.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65257 XM_011513646.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65258 XM_011513648.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65259 XM_011513649.1
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu65260 XM_011513651.2
Latest version!
Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu100276 NM_001330784.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100280 NM_001330788.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu100283 NM_001330787.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu100284 NM_001330786.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu06760 NM_014988.3 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$544.50-$762.30
$1089.00
OHu38528 NM_001330672.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu38534 NM_001330790.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38536 NM_001330791.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu06760 NM_014988.4 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$544.50-$762.30
$1089.00
OHu100274 NM_001330789.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu06336 NM_001112717.3 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38537 NM_001330792.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu06340 NM_001112718.3 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $741.30
$1059.00
OHu06603 NM_001289124.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu06600 NM_001289122.2 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu06760
    Clone ID Related Accession (Same CDS sequence) NM_014988.3 , NM_014988.5 , NM_014988.4
    Accession Version NM_014988.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product LIM and calponin homology domains-containing protein 1 isoform a
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055803.2
    Translation

    Target ORF information:

    RefSeq Version NM_014988.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014988.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06760
    Clone ID Related Accession (Same CDS sequence) NM_014988.3 , NM_014988.5 , NM_014988.4
    Accession Version NM_014988.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On Jun 1, 2019 this sequence version replaced NM_014988.4. Transcript Variant: This variant (1) encodes isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.165127.1, SRR1803615.110090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055803.2
    CDS35..3286
    Translation

    Target ORF information:

    RefSeq Version NM_014988.5
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014988.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06760
    Clone ID Related Accession (Same CDS sequence) NM_014988.3 , NM_014988.5 , NM_014988.4
    Accession Version NM_014988.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_014988.3. Transcript Variant: This variant (1) encodes isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.165127.1, AB029025.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055803.2
    CDS211..3462
    Translation

    Target ORF information:

    RefSeq Version NM_014988.4
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014988.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06341
    Clone ID Related Accession (Same CDS sequence) NM_001112719.3
    Accession Version NM_001112719.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform d
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, CR749205.1, CR936661.1, AC105389.3, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112719.2. Transcript Variant: This variant (4) differs in the 5' UTR and coding region and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: CR749205.1, SRR1660807.249952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106190.1
    CDS367..3075
    Translation

    Target ORF information:

    RefSeq Version NM_001112719.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112719.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06342
    Clone ID Related Accession (Same CDS sequence) NM_001112720.3
    Accession Version NM_001112720.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform e
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, DA786971.1, BX537916.1, AC105389.3, CR936664.1, CR749205.1 and AK026815.1. On Sep 3, 2016 this sequence version replaced NM_001112720.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding region, uses an alternate in-frame splice site, and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (e) has a shorter and distinct N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BX537916.1, AK299297.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106191.1
    CDS367..3039
    Translation

    Target ORF information:

    RefSeq Version NM_001112720.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06603
    Clone ID Related Accession (Same CDS sequence) NM_001289124.1 , NM_001289124.2
    Accession Version NM_001289124.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform g
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936610.1, BX537916.1, CR936601.1, CR749205.1 and AK026815.1. ##Evidence-Data-START## Transcript exon combination :: CR936610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC
    ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG
    TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276053.1
    CDS579..3311
    Translation

    Target ORF information:

    RefSeq Version NM_001289124.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC
    ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG
    TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06603
    Clone ID Related Accession (Same CDS sequence) NM_001289124.1 , NM_001289124.2
    Accession Version NM_001289124.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform g
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936610.1, BX537916.1, CR936601.1, CR749205.1 and AK026815.1. On May 31, 2019 this sequence version replaced NM_001289124.1. ##Evidence-Data-START## Transcript exon combination :: CR936610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC
    ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG
    TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276053.1
    CDS579..3311
    Translation

    Target ORF information:

    RefSeq Version NM_001289124.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289124.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC
    ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG
    TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65265
    Clone ID Related Accession (Same CDS sequence) NM_001330983.1
    Accession Version NM_001330983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform t
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_011513656.1. Transcript Variant: This variant (20) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.104390.1, SRR1660809.31958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317912.1
    CDS174..2969
    Translation

    Target ORF information:

    RefSeq Version NM_001330983.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65266
    Clone ID Related Accession (Same CDS sequence) NM_001330674.1
    Accession Version NM_001330674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform i
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, AK299835.1, CR936658.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 1, 2016 this sequence version replaced XM_011513657.1. ##Evidence-Data-START## Transcript exon combination :: AK294774.1, AK299835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG
    GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317603.1
    CDS367..3117
    Translation

    Target ORF information:

    RefSeq Version NM_001330674.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG
    GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65264
    Clone ID Related Accession (Same CDS sequence) XM_011513655.1
    Accession Version XM_011513655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC
    CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TATGACACCT
    CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511957.1
    CDS178..3015
    Translation

    Target ORF information:

    RefSeq Version XM_011513655.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC
    AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC
    CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT
    GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG
    GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG
    AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG
    CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC
    ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG
    GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG
    AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC
    ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA
    ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT
    TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG
    GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA
    ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA
    CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC
    GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA
    TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG
    ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT
    GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG
    TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA
    TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT
    CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC
    CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TATGACACCT
    CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65267
    Clone ID Related Accession (Same CDS sequence) XM_011513658.2
    Accession Version XM_011513658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511960.1
    CDS795..3452
    Translation

    Target ORF information:

    RefSeq Version XM_011513658.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG
    CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG
    CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG
    AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA
    ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC
    TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG
    AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC
    ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA
    ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA
    CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT
    GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA
    GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG
    AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG
    GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG
    TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC
    ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG
    AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT
    ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG
    ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA
    AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA
    AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC
    AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100276
    Clone ID Related Accession (Same CDS sequence) NM_001330784.2 , NM_001330784.1
    Accession Version NM_001330784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform j
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936658.1, AC108050.5, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001330784.1. ##Evidence-Data-START## Transcript exon combination :: CR936658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317713.1
    CDS35..2977
    Translation

    Target ORF information:

    RefSeq Version NM_001330784.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100276
    Clone ID Related Accession (Same CDS sequence) NM_001330784.2 , NM_001330784.1
    Accession Version NM_001330784.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-14
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform j
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, CR936658.1, AC108050.5, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. ##Evidence-Data-START## Transcript exon combination :: CR936658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317713.1
    CDS211..3153
    Translation

    Target ORF information:

    RefSeq Version NM_001330784.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100278
    Clone ID Related Accession (Same CDS sequence) NM_001330982.1
    Accession Version NM_001330982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform s
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007905.1. Transcript Variant: This variant (19) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC
    AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG
    GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA
    CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT
    TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA
    ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA
    TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG
    GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA
    GTCCATGAAG ACCATCAGCT GGATACCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA
    CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA
    GATGTGAAGC CAAAAACCCT CCCGCTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC
    AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317911.1
    CDS367..3078
    Translation

    Target ORF information:

    RefSeq Version NM_001330982.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC
    AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG
    GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA
    CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT
    TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA
    ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA
    TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG
    GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA
    GTCCATGAAG ACCATCAGCT GGATACCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA
    CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA
    GATGTGAAGC CAAAAACCCT CCCGCTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC
    AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG
    GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC
    AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT
    CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT
    ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100281
    Clone ID Related Accession (Same CDS sequence) NM_001330793.1
    Accession Version NM_001330793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform r
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007904.1. Transcript Variant: This variant (18) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.58248.1, SRR1660807.73523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC
    TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT
    CACATCCAGT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGTGGG
    ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA
    AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317722.1
    CDS367..3153
    Translation

    Target ORF information:

    RefSeq Version NM_001330793.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC
    TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT
    CACATCCAGT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGTGGG
    ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA
    AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100284
    Clone ID Related Accession (Same CDS sequence) NM_001330786.2 , NM_001330786.1
    Accession Version NM_001330786.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform k
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, BC023546.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001330786.1. Transcript Variant: This variant (11) represents use of an alternate promoter and initiates translation at a downstream start codon compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: BC023546.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG
    TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA
    GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT
    CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA
    GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA
    GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC
    CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA
    GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA
    GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG
    GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC
    GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA
    AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG
    GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA
    CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC
    TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT
    GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA
    GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA
    CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG
    ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317715.1
    CDS404..3097
    Translation

    Target ORF information:

    RefSeq Version NM_001330786.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330786.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG
    TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA
    GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT
    CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA
    GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA
    GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC
    CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA
    GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA
    GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG
    GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC
    GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA
    AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG
    GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA
    CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC
    TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT
    GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA
    GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA
    CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG
    ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100284
    Clone ID Related Accession (Same CDS sequence) NM_001330786.2 , NM_001330786.1
    Accession Version NM_001330786.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-14
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform k
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, BC023546.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. Transcript Variant: This variant (11) represents use of an alternate promoter and initiates translation at a downstream start codon compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: BC023546.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG
    TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA
    GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT
    CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA
    GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA
    GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC
    CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA
    GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA
    GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG
    GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC
    GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA
    AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG
    GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA
    CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC
    TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT
    GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA
    GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA
    CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG
    ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317715.1
    CDS404..3097
    Translation

    Target ORF information:

    RefSeq Version NM_001330786.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG
    TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG
    CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA
    GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT
    CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA
    GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA
    GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA
    GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC
    TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC
    CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC
    ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA
    GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG
    CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA
    GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG
    GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC
    GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA
    AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG
    GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA
    CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC
    TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT
    GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA
    GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA
    CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG
    ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105497
    Clone ID Related Accession (Same CDS sequence) XM_024453938.1
    Accession Version XM_024453938.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TGAGAGATAC CAGAAGGAGC AGGACAAGCT GAAAGAAGAG
    TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG GAAGAACGCA GATACTATGA GGAGGAGCGT
    AAGATAATTG AAGACACTGT GGTTCCATTT ACTGTTTCTT CAAGTTCCGC TGACCAGCTG
    TCTACCTCTT CCTCCATGAC TGAAGGCAGT GGGACAATGA ATAAGATAGA CCTGGGAAAC
    TGTCAAGATG AAAAACAAGA CAGAAGATGG AAGAAATCAT TCCAGGGAGA TGACAGTGAC
    TTATTGCTGA AGACTAGGGA AAGTGATCGA CTGGAGGAGA AGGGCAGCCT AACTGAAGGG
    GCCTTGGCTC ATTCTGGGAA CCCTGTATCA AAAGGAGTCC ATGAAGACCA TCAGCTGGAT
    ACCGAGGCTG GGGCCCCACA CTGTGGAACA AACCCACAGC TTGCTCAGGA TCCATCCCAG
    AATCAGCAGA CATCAAATCC AACGCACAGT TCAGAAGATG TGAAGCCAAA AACCCTCCCG
    CTGGATAAAA GCATTAACCA TCAGATCGAG TCTCCCAGTG AAAGGCGGAA GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309706.1
    CDS178..2805
    Translation

    Target ORF information:

    RefSeq Version XM_024453938.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG
    AAGTCACCTG TTATGACACC TGAGAGATAC CAGAAGGAGC AGGACAAGCT GAAAGAAGAG
    TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG GAAGAACGCA GATACTATGA GGAGGAGCGT
    AAGATAATTG AAGACACTGT GGTTCCATTT ACTGTTTCTT CAAGTTCCGC TGACCAGCTG
    TCTACCTCTT CCTCCATGAC TGAAGGCAGT GGGACAATGA ATAAGATAGA CCTGGGAAAC
    TGTCAAGATG AAAAACAAGA CAGAAGATGG AAGAAATCAT TCCAGGGAGA TGACAGTGAC
    TTATTGCTGA AGACTAGGGA AAGTGATCGA CTGGAGGAGA AGGGCAGCCT AACTGAAGGG
    GCCTTGGCTC ATTCTGGGAA CCCTGTATCA AAAGGAGTCC ATGAAGACCA TCAGCTGGAT
    ACCGAGGCTG GGGCCCCACA CTGTGGAACA AACCCACAGC TTGCTCAGGA TCCATCCCAG
    AATCAGCAGA CATCAAATCC AACGCACAGT TCAGAAGATG TGAAGCCAAA AACCCTCCCG
    CTGGATAAAA GCATTAACCA TCAGATCGAG TCTCCCAGTG AAAGGCGGAA GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94982
    Clone ID Related Accession (Same CDS sequence) XM_017007903.1
    Accession Version XM_017007903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGT
    GTTCCGCTTA GAGTTCAGAA CTCTTGGCGA CGATCCCAGT TTTTCTCCCA GTCAGTGGAT
    TCTCCAAGCA GTGAGAAGTC ACCTGTTATG ACACCTCAGT TTAAGTTCTG GGCATGGGAC
    CCAGAAGAGG AGCGCAGGCG ACAGGAAAAA TGGCAACAGG AACAGGAACG TTTGCTCCAG
    GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG
    GTGGAAGAGG AAGAACGCAG ATACTATGAG GAGGAGCGTA AGATAATTGA AGACACTGTG
    GTTCCATTTA CTGTTTCTTC AAGTTCCGCT GACCAGCTGT CTACCTCTTC CTCCATGACT
    GAAGGCAGTG GGACAATGAA TAAGATAGAC CTGGGAAACT GTCAAGATGA AAAACAAGAC
    AGAAGATGGA AGAAATCATT CCAGGGAGAT GACAGTGACT TATTGCTGAA GACTAGGGAA
    AGTGATCGAC TGGAGGAGAA GGGCAGCCTA ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC
    CCTGTATCAA AAGGAGTCCA TGAAGACCAT CAGCTGGATA CCGAGGCTGG GGCCCCACAC
    TGTGGAACAA ACCCACAGCT TGCTCAGGAT CCATCCCAGA ATCAGCAGAC ATCAAATCCA
    ACGCACAGTT CAGAAGATGT GAAGCCAAAA ACCCTCCCGC TGGATAAAAG CATTAACCAT
    CAGATCGAGT CTCCCAGTGA AAGGCGGAAG AAAAGTCCCC GAGAGCACTT CCAGGCTGGG
    CCTTTCTCTC CCTGTTCTCC CACCCCTCCC GGTCAGTCAC CAAACAGGTA CAAGAGGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863392.1
    CDS178..3051
    Translation

    Target ORF information:

    RefSeq Version XM_017007903.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT
    CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG
    GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT
    AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT
    AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG
    TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA
    ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG
    TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG
    GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA
    AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG
    CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT
    ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT
    ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT
    AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA
    AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT
    GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC
    AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA
    CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT
    GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC
    TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG
    AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA
    AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT
    GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGT
    GTTCCGCTTA GAGTTCAGAA CTCTTGGCGA CGATCCCAGT TTTTCTCCCA GTCAGTGGAT
    TCTCCAAGCA GTGAGAAGTC ACCTGTTATG ACACCTCAGT TTAAGTTCTG GGCATGGGAC
    CCAGAAGAGG AGCGCAGGCG ACAGGAAAAA TGGCAACAGG AACAGGAACG TTTGCTCCAG
    GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG
    GTGGAAGAGG AAGAACGCAG ATACTATGAG GAGGAGCGTA AGATAATTGA AGACACTGTG
    GTTCCATTTA CTGTTTCTTC AAGTTCCGCT GACCAGCTGT CTACCTCTTC CTCCATGACT
    GAAGGCAGTG GGACAATGAA TAAGATAGAC CTGGGAAACT GTCAAGATGA AAAACAAGAC
    AGAAGATGGA AGAAATCATT CCAGGGAGAT GACAGTGACT TATTGCTGAA GACTAGGGAA
    AGTGATCGAC TGGAGGAGAA GGGCAGCCTA ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC
    CCTGTATCAA AAGGAGTCCA TGAAGACCAT CAGCTGGATA CCGAGGCTGG GGCCCCACAC
    TGTGGAACAA ACCCACAGCT TGCTCAGGAT CCATCCCAGA ATCAGCAGAC ATCAAATCCA
    ACGCACAGTT CAGAAGATGT GAAGCCAAAA ACCCTCCCGC TGGATAAAAG CATTAACCAT
    CAGATCGAGT CTCCCAGTGA AAGGCGGAAG AAAAGTCCCC GAGAGCACTT CCAGGCTGGG
    CCTTTCTCTC CCTGTTCTCC CACCCCTCCC GGTCAGTCAC CAAACAGGTA CAAGAGGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100274
    Clone ID Related Accession (Same CDS sequence) NM_001330789.2 , NM_001330789.1
    Accession Version NM_001330789.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform n
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330789.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97937.1, SRR1803614.108979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317718.1
    CDS35..3124
    Translation

    Target ORF information:

    RefSeq Version NM_001330789.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330789.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100274
    Clone ID Related Accession (Same CDS sequence) NM_001330789.2 , NM_001330789.1
    Accession Version NM_001330789.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform n
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007902.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97937.1, SRR1660807.109176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317718.1
    CDS211..3300
    Translation

    Target ORF information:

    RefSeq Version NM_001330789.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100280
    Clone ID Related Accession (Same CDS sequence) NM_001330788.1 , NM_001330788.2
    Accession Version NM_001330788.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform m
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007901.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.237697.1, SRR1660803.88863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317717.1
    CDS211..3378
    Translation

    Target ORF information:

    RefSeq Version NM_001330788.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100280
    Clone ID Related Accession (Same CDS sequence) NM_001330788.1 , NM_001330788.2
    Accession Version NM_001330788.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform m
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330788.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.156655.1, SRR1660803.88863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317717.1
    CDS35..3202
    Translation

    Target ORF information:

    RefSeq Version NM_001330788.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330788.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100283
    Clone ID Related Accession (Same CDS sequence) NM_001330787.2 , NM_001330787.1
    Accession Version NM_001330787.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform l
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330787.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.214498.1, SRR1660803.230038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317716.1
    CDS35..3292
    Translation

    Target ORF information:

    RefSeq Version NM_001330787.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330787.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100283
    Clone ID Related Accession (Same CDS sequence) NM_001330787.2 , NM_001330787.1
    Accession Version NM_001330787.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform l
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007899.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.230038.1, SRR1803611.214498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317716.1
    CDS211..3468
    Translation

    Target ORF information:

    RefSeq Version NM_001330787.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT
    CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG
    TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT
    AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG
    CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105496
    Clone ID Related Accession (Same CDS sequence) XM_024453937.1
    Accession Version XM_024453937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309705.1
    CDS57..3281
    Translation

    Target ORF information:

    RefSeq Version XM_024453937.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94980
    Clone ID Related Accession (Same CDS sequence) XM_017007898.1
    Accession Version XM_017007898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863387.1
    CDS57..3317
    Translation

    Target ORF information:

    RefSeq Version XM_017007898.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA
    AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT
    CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94981
    Clone ID Related Accession (Same CDS sequence) XM_017007900.1
    Accession Version XM_017007900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTACAAGAGG
    TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA
    ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT
    AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG
    AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863389.1
    CDS57..3233
    Translation

    Target ORF information:

    RefSeq Version XM_017007900.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT
    GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTACAAGAGG
    TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA
    ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT
    AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG
    AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06336
    Clone ID Related Accession (Same CDS sequence) NM_001112717.3 , NM_001112717.4
    Accession Version NM_001112717.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR933645.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112717.2. ##Evidence-Data-START## Transcript exon combination :: CR933645.1, SRR1803612.87314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106188.1
    CDS211..3384
    Translation

    Target ORF information:

    RefSeq Version NM_001112717.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112717.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06336
    Clone ID Related Accession (Same CDS sequence) NM_001112717.3 , NM_001112717.4
    Accession Version NM_001112717.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR933645.1, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001112717.3. ##Evidence-Data-START## Transcript exon combination :: CR933645.1, SRR1803612.87314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106188.1
    CDS35..3208
    Translation

    Target ORF information:

    RefSeq Version NM_001112717.4
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112717.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG
    CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA
    GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG
    GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC
    CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG
    GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC
    AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT
    GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG
    CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA
    TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC
    CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT
    ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG
    ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA
    GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC
    TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06340
    Clone ID Related Accession (Same CDS sequence) NM_001112718.3 , NM_001112718.4
    Accession Version NM_001112718.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR936664.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112718.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.22792.1, SRR1803616.158804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106189.1
    CDS211..3381
    Translation

    Target ORF information:

    RefSeq Version NM_001112718.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112718.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06340
    Clone ID Related Accession (Same CDS sequence) NM_001112718.3 , NM_001112718.4
    Accession Version NM_001112718.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR936664.1, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001112718.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.158804.1, SRR1803617.199715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106189.1
    CDS35..3205
    Translation

    Target ORF information:

    RefSeq Version NM_001112718.4
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112718.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06600
    Clone ID Related Accession (Same CDS sequence) NM_001289122.2 , NM_001289122.3
    Accession Version NM_001289122.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform f
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, CR936658.1, AB029025.2, CR936601.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001289122.1. ##Evidence-Data-START## Transcript exon combination :: CR936601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG
    GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC
    CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276051.1
    CDS211..3420
    Translation

    Target ORF information:

    RefSeq Version NM_001289122.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289122.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG
    GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC
    CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06600
    Clone ID Related Accession (Same CDS sequence) NM_001289122.2 , NM_001289122.3
    Accession Version NM_001289122.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform f
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936658.1, AB029025.2, CR936601.1, CR749205.1, AK026815.1 and BX537916.1. On Jun 1, 2019 this sequence version replaced NM_001289122.2. ##Evidence-Data-START## Transcript exon combination :: CR936601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG
    GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC
    CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276051.1
    CDS35..3244
    Translation

    Target ORF information:

    RefSeq Version NM_001289122.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289122.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG
    GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC
    CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG
    GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT
    GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG
    ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA
    GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG
    GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG
    AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA
    CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT
    CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC
    CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38534
    Clone ID Related Accession (Same CDS sequence) NM_001330790.1 , NM_001330790.2
    Accession Version NM_001330790.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform o
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248072.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.261334.1, SRR1803617.15300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317719.1
    CDS211..3459
    Translation

    Target ORF information:

    RefSeq Version NM_001330790.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38534
    Clone ID Related Accession (Same CDS sequence) NM_001330790.1 , NM_001330790.2
    Accession Version NM_001330790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform o
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330790.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.61307.1, SRR1803615.136930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317719.1
    CDS35..3283
    Translation

    Target ORF information:

    RefSeq Version NM_001330790.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA
    CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA
    CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA
    GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG
    CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG
    CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA
    AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT
    GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA
    GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG
    GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC
    CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC
    CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT
    CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG
    TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38536
    Clone ID Related Accession (Same CDS sequence) NM_001330791.2 , NM_001330791.1
    Accession Version NM_001330791.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform p
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330791.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.155570.1, SRR1803613.97206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC
    TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317720.1
    CDS35..3247
    Translation

    Target ORF information:

    RefSeq Version NM_001330791.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330791.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC
    TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38536
    Clone ID Related Accession (Same CDS sequence) NM_001330791.2 , NM_001330791.1
    Accession Version NM_001330791.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform p
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248074.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.97206.1, SRR1660807.155570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC
    TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317720.1
    CDS211..3423
    Translation

    Target ORF information:

    RefSeq Version NM_001330791.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC
    TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38537
    Clone ID Related Accession (Same CDS sequence) NM_001330792.1 , NM_001330792.2
    Accession Version NM_001330792.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform q
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248075.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.33203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT
    TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT
    TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG
    ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG
    CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317721.1
    CDS211..3345
    Translation

    Target ORF information:

    RefSeq Version NM_001330792.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT
    TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT
    TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG
    ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG
    CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38537
    Clone ID Related Accession (Same CDS sequence) NM_001330792.1 , NM_001330792.2
    Accession Version NM_001330792.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform q
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Jun 1, 2019 this sequence version replaced NM_001330792.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.33203.1, SRR7346977.1546087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT
    TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT
    TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG
    ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG
    CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317721.1
    CDS35..3169
    Translation

    Target ORF information:

    RefSeq Version NM_001330792.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330792.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG
    CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG
    AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA
    GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT
    GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG
    ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG
    AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG
    GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA
    GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC
    CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA
    GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT
    CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT
    TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT
    TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG
    ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG
    CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65262
    Clone ID Related Accession (Same CDS sequence) XM_011513653.1
    Accession Version XM_011513653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG
    AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511955.1
    CDS57..3392
    Translation

    Target ORF information:

    RefSeq Version XM_011513653.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT
    GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG
    CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG
    GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG
    GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT
    GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG
    CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG
    GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG
    GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG
    AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT
    GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC
    AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT
    TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC
    AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG
    GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG
    TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC
    TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC
    AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG
    CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC
    AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA
    ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA
    CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT
    TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG
    AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG
    CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG
    CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC
    AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT
    TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG
    AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA
    TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG
    AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC
    CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG
    CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT
    GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT
    GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT
    CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65263
    Clone ID Related Accession (Same CDS sequence) XM_011513654.1
    Accession Version XM_011513654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTCAGTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG
    GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG
    AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT
    GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AGTGTTTCAG GTGTGGAATT
    TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT GGGACGGATG TTAGGATTCG AAATGGTCTC
    CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511956.1
    CDS57..3356
    Translation

    Target ORF information:

    RefSeq Version XM_011513654.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG
    TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG
    ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG
    AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG
    GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC
    AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC
    GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC
    AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA
    GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG
    TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT
    CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA
    GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG
    ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG
    AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT
    GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC
    GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA
    ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA
    GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC
    GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA
    GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG
    GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA
    ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT
    GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA
    GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTCAGTTTAA GTTCTGGGCA
    TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG
    CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA
    AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC
    ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC
    ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA
    CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT
    AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT
    GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC
    CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA
    AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT
    AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG
    GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG
    AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT
    GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AGTGTTTCAG GTGTGGAATT
    TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT GGGACGGATG TTAGGATTCG AAATGGTCTC
    CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94976
    Clone ID Related Accession (Same CDS sequence) XM_017007894.1
    Accession Version XM_017007894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863383.1
    CDS57..5012
    Translation

    Target ORF information:

    RefSeq Version XM_017007894.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT CTATAAGTGG AAAGAAGCTG
    TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT
    CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA
    GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG
    CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94977
    Clone ID Related Accession (Same CDS sequence) XM_017007895.1
    Accession Version XM_017007895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863384.1
    CDS57..4940
    Translation

    Target ORF information:

    RefSeq Version XM_017007895.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94978
    Clone ID Related Accession (Same CDS sequence) XM_017007896.1
    Accession Version XM_017007896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG
    AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG
    GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT
    GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC
    CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT
    GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA
    ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT
    CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT
    CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA
    ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG
    TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA
    ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT
    AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG
    AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863385.1
    CDS57..4853
    Translation

    Target ORF information:

    RefSeq Version XM_017007896.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG
    AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG
    GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT
    GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC
    CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT
    GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA
    ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT
    CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT
    CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA
    ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG
    TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA
    ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT
    AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG
    AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94979
    Clone ID Related Accession (Same CDS sequence) XM_017007897.1
    Accession Version XM_017007897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA
    AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA
    ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC
    TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA
    GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG
    CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG
    GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG
    GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG
    CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT
    AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG
    AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG GGTAAAGGAG CTGCAATGAT CATCGAGACC
    CTCAATCTCT ATTTTCACAT CCAGTGTTTC AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA
    GATGCAGTGA GTGGGACGGA TGTTAGGATT CGAAATGGTC TCCTGAACTG TAATGATTGC
    TACATGCGAT CCAGAAGTGC CGGGCAGCCT ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863386.1
    CDS57..4778
    Translation

    Target ORF information:

    RefSeq Version XM_017007897.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA
    AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA
    ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC
    TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA
    GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG
    CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG
    GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG
    GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG
    CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT
    AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG
    AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG GGTAAAGGAG CTGCAATGAT CATCGAGACC
    CTCAATCTCT ATTTTCACAT CCAGTGTTTC AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA
    GATGCAGTGA GTGGGACGGA TGTTAGGATT CGAAATGGTC TCCTGAACTG TAATGATTGC
    TACATGCGAT CCAGAAGTGC CGGGCAGCCT ACAACATTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38528
    Clone ID Related Accession (Same CDS sequence) NM_001330672.1 , NM_001330672.2
    Accession Version NM_001330672.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-27
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform h
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, AL831962.2, AC108050.5, AC124009.4, AC105389.3, AB029025.2, CR749205.1, AK026815.1 and BX537916.1. On Sep 1, 2016 this sequence version replaced XM_005248067.2. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream start codon and multiple internal differences, including a large novel segment. The resulting isoform (h) has a longer overall length, but has a shorter N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL831962.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317601.1
    CDS435..4838
    Translation

    Target ORF information:

    RefSeq Version NM_001330672.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38528
    Clone ID Related Accession (Same CDS sequence) NM_001330672.1 , NM_001330672.2
    Accession Version NM_001330672.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform h
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, AL831962.2, AC108050.5, AC124009.4, AC105389.3, AB029025.2, CR749205.1, AK026815.1 and BX537916.1. On Nov 22, 2018 this sequence version replaced NM_001330672.1. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream start codon and multiple internal differences, including a large novel segment. The resulting isoform (h) has a longer overall length, but has a shorter N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL831962.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000503057.6/ ENSP00000425631.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317601.1
    CDS435..4838
    Translation

    Target ORF information:

    RefSeq Version NM_001330672.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG
    TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38518
    Clone ID Related Accession (Same CDS sequence) XM_005248057.1
    Accession Version XM_005248057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248114.1
    CDS57..4949
    Translation

    Target ORF information:

    RefSeq Version XM_005248057.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA
    CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG
    GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA
    TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA
    AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT
    AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC
    CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG
    GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT
    GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT
    GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG
    AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA
    AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC
    ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC
    TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC
    TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG
    AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA
    TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38519
    Clone ID Related Accession (Same CDS sequence) XM_005248058.1
    Accession Version XM_005248058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC
    CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248115.1
    CDS57..4946
    Translation

    Target ORF information:

    RefSeq Version XM_005248058.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC
    CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT
    TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT
    TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT
    GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC
    AGAAGTGCCG GGCAGCCTAC AACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38521
    Clone ID Related Accession (Same CDS sequence) XM_005248060.1
    Accession Version XM_005248060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC
    CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248117.1
    CDS57..4937
    Translation

    Target ORF information:

    RefSeq Version XM_005248060.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC
    CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38522
    Clone ID Related Accession (Same CDS sequence) XM_005248061.1
    Accession Version XM_005248061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA
    AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA
    ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC
    TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA
    GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG
    CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG
    GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG
    GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG
    CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT
    AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGAAAAG TCCCCGAGAG
    CACTTCCAGG CTGGGCCTTT CTCTCCCTGT TCTCCCACCC CTCCCGGTCA GTCACCAAAC
    AGGTACAAGA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248118.1
    CDS57..4865
    Translation

    Target ORF information:

    RefSeq Version XM_005248061.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA
    AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA
    ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC
    TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA
    GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG
    CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG
    GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG
    GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG
    CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT
    AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGAAAAG TCCCCGAGAG
    CACTTCCAGG CTGGGCCTTT CTCTCCCTGT TCTCCCACCC CTCCCGGTCA GTCACCAAAC
    AGGTACAAGA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT
    AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG
    TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA
    AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA
    ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38524
    Clone ID Related Accession (Same CDS sequence) XM_006713996.1
    Accession Version XM_006713996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714059.1
    CDS57..4859
    Translation

    Target ORF information:

    RefSeq Version XM_006713996.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006713996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA
    CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG
    GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC
    AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC
    TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT
    TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG
    ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG
    GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC
    AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA
    GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT
    CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG
    CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG
    CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA
    GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA
    ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT
    CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65253
    Clone ID Related Accession (Same CDS sequence) XM_011513642.1
    Accession Version XM_011513642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC
    TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511944.1
    CDS57..5024
    Translation

    Target ORF information:

    RefSeq Version XM_011513642.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC
    TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT
    GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC
    GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG
    CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT
    GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65254
    Clone ID Related Accession (Same CDS sequence) XM_011513643.1
    Accession Version XM_011513643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511945.1
    CDS57..5021
    Translation

    Target ORF information:

    RefSeq Version XM_011513643.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65255
    Clone ID Related Accession (Same CDS sequence) XM_011513644.1
    Accession Version XM_011513644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC
    TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG
    CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC
    AATCTCTATT TTCACATCCA GTGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT
    GCAGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC
    ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511946.1
    CDS57..5015
    Translation

    Target ORF information:

    RefSeq Version XM_011513644.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC
    TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG
    CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC
    AATCTCTATT TTCACATCCA GTGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT
    GCAGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC
    ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65256
    Clone ID Related Accession (Same CDS sequence) XM_011513645.1
    Accession Version XM_011513645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG
    AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG
    GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT
    GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC
    CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT
    GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA
    ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT
    CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT
    CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA
    ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG
    AAAAGTCCCC GAGAGCACTT CCAGGCTGGG CCTTTCTCTC CCTGTTCTCC CACCCCTCCC
    GGTCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511947.1
    CDS57..4940
    Translation

    Target ORF information:

    RefSeq Version XM_011513645.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG
    AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG
    GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT
    GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC
    CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT
    GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA
    ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT
    CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT
    CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA
    ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG
    AAAAGTCCCC GAGAGCACTT CCAGGCTGGG CCTTTCTCTC CCTGTTCTCC CACCCCTCCC
    GGTCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT
    GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC
    ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG
    GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT
    GCCGGGCAGC CTACAACATT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65257
    Clone ID Related Accession (Same CDS sequence) XM_011513646.1
    Accession Version XM_011513646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511948.1
    CDS57..4937
    Translation

    Target ORF information:

    RefSeq Version XM_011513646.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT
    CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC
    CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT
    ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA
    AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA
    GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT
    TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA
    TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT
    CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA
    AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT
    GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC
    TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG
    GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA
    AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA
    GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA
    GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG
    TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA
    CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA
    ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG
    GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG
    CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA
    AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG
    GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT
    CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG
    GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT
    CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA
    TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA
    CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC
    ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG
    ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA
    GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG
    TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC
    AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA
    AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT
    AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA
    TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG
    GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA
    CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA
    AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC
    AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG
    CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA
    AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA
    GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA
    TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA
    GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA
    TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC
    AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA
    TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT
    CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA
    TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA
    ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC
    AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC
    GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG
    CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC
    CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT
    GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC
    GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65258
    Clone ID Related Accession (Same CDS sequence) XM_011513648.1
    Accession Version XM_011513648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA
    GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC
    AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGCTAGATCA GCAGAAATGG
    AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT
    GGCAGCAAGA TTCAAATGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG
    GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTGTGG CCGAGGTGAC
    TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC
    AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAAACAGCT GCCACAGGAT
    GGCAAAGAAG AAACAGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA
    TCAGAGGACA GCAGCCAGCC ACTGGTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG
    ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC
    CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC
    CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG
    GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT
    CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC
    GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG
    AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT
    GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT
    GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA
    GAAAAAGAGC GGAGAGAGAG AGAGCTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG
    GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTC
    GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC
    TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC
    AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG
    TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA
    CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAC
    GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT
    CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA
    GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG
    AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT
    CAAGAGGACA AGAATGATGG TGGAAAATCA AGAAAAGGGA ATATAGAACT TGCCTCATCA
    GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA
    TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA
    GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA
    TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC
    CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT
    AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511950.1
    CDS57..4781
    Translation

    Target ORF information:

    RefSeq Version XM_011513648.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
    GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT
    GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT
    ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC
    AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT
    GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT
    AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC
    ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG
    CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC
    GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT
    TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA
    GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG
    AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG
    AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC
    CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC
    GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG
    GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG
    AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA
    GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA
    AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC
    AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA
    AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT
    CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC
    AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG
    AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT
    AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA
    GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC
    AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGCTAGATCA GCAGAAATGG
    AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT
    GGCAGCAAGA TTCAAATGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG
    GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTGTGG CCGAGGTGAC
    TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC
    AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAAACAGCT GCCACAGGAT
    GGCAAAGAAG AAACAGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA
    TCAGAGGACA GCAGCCAGCC ACTGGTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG
    ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC
    CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC
    CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG
    GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT
    CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC
    GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG
    AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT
    GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT
    GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA
    GAAAAAGAGC GGAGAGAGAG AGAGCTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG
    GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTC
    GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC
    TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC
    AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG
    TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA
    CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAC
    GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT
    CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA
    GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG
    AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT
    CAAGAGGACA AGAATGATGG TGGAAAATCA AGAAAAGGGA ATATAGAACT TGCCTCATCA
    GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA
    TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA
    GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA
    TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC
    CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT
    AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC
    AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG
    GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC
    CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG
    GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT
    ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT
    GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG
    AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA
    CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA
    AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA
    AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT
    TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG
    TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT
    CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA
    AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG
    ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT
    GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT
    TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65259
    Clone ID Related Accession (Same CDS sequence) XM_011513649.1
    Accession Version XM_011513649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT
    GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGCTGGC
    CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT
    AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA
    GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA
    GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT
    ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA
    GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA
    GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT
    CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA
    AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG
    ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA
    GATCTTCACA AGGATGACCT GGCCCAGCAG AGAATTCAGG GCAGCCTTGC CCCTCACCGC
    GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG
    AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG
    CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA
    AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG
    GAGCTAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT
    GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAATGG ACTCTGTGTC TCCTGTCTCA
    GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA
    ATGAATTGTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG
    TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA
    TCCAAACAGC TGCCACAGGA TGGCAAAGAA GAAACAGAAA GCGCTCCAAG AGATTCTGAG
    AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGGTGTG TCCTCTGGCC
    TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG
    AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG
    GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA
    GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT
    CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT
    TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA
    AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGCTGCA TGAAGCATAT
    AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC
    ACCATCAGTG AGGCTGTTCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG
    CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC
    AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT
    CTGAAGACCT TTAAGGTAGA CGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG
    GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG
    ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT
    AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT
    GAGAAAACGG AACCGAATAG TCAAGAGGAC AAGAATGATG GTGGAAAATC AAGAAAAGGG
    AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC
    CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA
    GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA
    CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG
    GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT
    GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC
    CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA TGACACCTCA GTTTAAGTTC
    TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG
    GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT
    GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT
    TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT
    GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG
    AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT
    CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TACCGAGGCT
    GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG
    ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC GCTGGATAAA
    AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC
    TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG
    TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA
    GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAGTG TTTCAGGTGT
    GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGTGGGA CGGATGTTAG GATTCGAAAT
    GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511951.1
    CDS178..4683
    Translation

    Target ORF information:

    RefSeq Version XM_011513649.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
    CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT
    CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG
    ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA
    TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG
    ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC
    AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC
    CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT
    GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGCTGGC
    CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT
    AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA
    GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA
    GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT
    ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA
    GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA
    GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT
    CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA
    AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG
    ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA
    GATCTTCACA AGGATGACCT GGCCCAGCAG AGAATTCAGG GCAGCCTTGC CCCTCACCGC
    GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG
    AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG
    CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA
    AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG
    GAGCTAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT
    GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAATGG ACTCTGTGTC TCCTGTCTCA
    GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA
    ATGAATTGTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG
    TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA
    TCCAAACAGC TGCCACAGGA TGGCAAAGAA GAAACAGAAA GCGCTCCAAG AGATTCTGAG
    AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGGTGTG TCCTCTGGCC
    TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG
    AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG
    GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA
    GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT
    CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC
    ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG
    CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT
    TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA
    AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA
    ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGCTGCA TGAAGCATAT
    AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC
    ACCATCAGTG AGGCTGTTCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT
    TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG
    CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC
    AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC
    AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT
    CTGAAGACCT TTAAGGTAGA CGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG
    GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG
    ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT
    AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT
    GAGAAAACGG AACCGAATAG TCAAGAGGAC AAGAATGATG GTGGAAAATC AAGAAAAGGG
    AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC
    CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA
    GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA
    CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG
    GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT
    GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC
    CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA TGACACCTCA GTTTAAGTTC
    TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA
    CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG
    GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT
    GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT
    TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT
    GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG
    AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT
    CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TACCGAGGCT
    GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG
    ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC GCTGGATAAA
    AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC
    TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG
    TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA
    GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAGTG TTTCAGGTGT
    GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGTGGGA CGGATGTTAG GATTCGAAAT
    GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65260
    Clone ID Related Accession (Same CDS sequence) XM_011513651.2
    Accession Version XM_011513651.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product LIM and calponin homology domains-containing protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT
    CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT
    CCAAGCAGTG AGAAGTCACC TGTTATGACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA
    GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG
    GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT
    CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA
    GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA
    AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT
    GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT
    GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATACCG AGGCTGGGGC CCCACACTGT
    GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG
    CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCGCTGG ATAAAAGCAT TAACCATCAG
    ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT
    TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511953.1
    CDS1413..5903
    Translation

    Target ORF information:

    RefSeq Version XM_011513651.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513651.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
    ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT
    GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT
    GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT
    CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG
    CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC
    GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT
    ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA
    GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT
    TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT
    TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC
    CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT
    GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA
    GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG
    TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA
    ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA
    GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA
    TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG
    GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT
    GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC
    ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA
    GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT
    AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA
    GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG
    AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC
    TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC
    TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA
    GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG
    GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA
    CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA
    GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT
    TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG
    AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG
    ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG
    CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG
    AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG
    GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC
    CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA
    AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA
    CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT
    GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC
    CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT
    GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT
    TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT
    CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC
    AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG
    CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG
    GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA
    GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG
    GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC
    ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG
    AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC
    TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT
    GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG
    AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG
    CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA
    GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT
    CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT
    CCAAGCAGTG AGAAGTCACC TGTTATGACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA
    GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG
    AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG
    GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT
    CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA
    GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA
    AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT
    GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT
    GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATACCG AGGCTGGGGC CCCACACTGT
    GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG
    CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCGCTGG ATAAAAGCAT TAACCATCAG
    ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT
    TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA
    AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC
    ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC
    CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT
    AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.