LIMCH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMCH1
Entrez Gene ID	22998
Full Name	LIM and calponin homology domains 1
Synonyms	LIMCH1A,LMO7B
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p13
Disorder MIM:	617750

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112717.3	NP_001106188.1	LIM and calponin homology domains-containing protein 1 isoform b
XM_011513644.1	XP_011511946.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_011513651.2	XP_011511953.1	LIM and calponin homology domains-containing protein 1 isoform X17
XM_011513653.1	XP_011511955.1	LIM and calponin homology domains-containing protein 1 isoform X18
NM_001330787.2	NP_001317716.1	LIM and calponin homology domains-containing protein 1 isoform l
NM_001330784.2	NP_001317713.1	LIM and calponin homology domains-containing protein 1 isoform j
NM_001330786.2	NP_001317715.1	LIM and calponin homology domains-containing protein 1 isoform k
XM_011513642.1	XP_011511944.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_005248057.1	XP_005248114.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_017007895.1	XP_016863384.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_005248060.1	XP_005248117.1	LIM and calponin homology domains-containing protein 1 isoform X9
XM_011513646.1	XP_011511948.1	LIM and calponin homology domains-containing protein 1 isoform X10
XM_005248061.1	XP_005248118.1	LIM and calponin homology domains-containing protein 1 isoform X11
XM_017007903.1	XP_016863392.1	LIM and calponin homology domains-containing protein 1 isoform X23
NM_001289124.1	NP_001276053.1	LIM and calponin homology domains-containing protein 1 isoform g
NM_001330788.1	NP_001317717.1	LIM and calponin homology domains-containing protein 1 isoform m
NM_001330784.1	NP_001317713.1	LIM and calponin homology domains-containing protein 1 isoform j
NM_001330788.2	NP_001317717.1	LIM and calponin homology domains-containing protein 1 isoform m
NM_001330982.1	NP_001317911.1	LIM and calponin homology domains-containing protein 1 isoform s
NM_001330793.1	NP_001317722.1	LIM and calponin homology domains-containing protein 1 isoform r
NM_001330789.2	NP_001317718.1	LIM and calponin homology domains-containing protein 1 isoform n
XM_011513645.1	XP_011511947.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_017007897.1	XP_016863386.1	LIM and calponin homology domains-containing protein 1 isoform X15
XM_017007898.1	XP_016863387.1	LIM and calponin homology domains-containing protein 1 isoform X20
XM_017007900.1	XP_016863389.1	LIM and calponin homology domains-containing protein 1 isoform X22
XM_011513655.1	XP_011511957.1	LIM and calponin homology domains-containing protein 1 isoform X24
NM_001112718.3	NP_001106189.1	LIM and calponin homology domains-containing protein 1 isoform c
NM_001330672.1	NP_001317601.1	LIM and calponin homology domains-containing protein 1 isoform h
NM_001330787.1	NP_001317716.1	LIM and calponin homology domains-containing protein 1 isoform l
NM_001330790.1	NP_001317719.1	LIM and calponin homology domains-containing protein 1 isoform o
NM_014988.3	NP_055803.2	LIM and calponin homology domains-containing protein 1 isoform a
NM_001330786.1	NP_001317715.1	LIM and calponin homology domains-containing protein 1 isoform k
NM_014988.5	NP_055803.2	LIM and calponin homology domains-containing protein 1 isoform a
NM_001330983.1	NP_001317912.1	LIM and calponin homology domains-containing protein 1 isoform t
NM_001330672.2	NP_001317601.1	LIM and calponin homology domains-containing protein 1 isoform h
NM_001330674.1	NP_001317603.1	LIM and calponin homology domains-containing protein 1 isoform i
NM_001330791.2	NP_001317720.1	LIM and calponin homology domains-containing protein 1 isoform p
NM_001330790.2	NP_001317719.1	LIM and calponin homology domains-containing protein 1 isoform o
XM_017007894.1	XP_016863383.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_011513649.1	XP_011511951.1	LIM and calponin homology domains-containing protein 1 isoform X16
XM_011513654.1	XP_011511956.1	LIM and calponin homology domains-containing protein 1 isoform X19
XM_024453937.1	XP_024309705.1	LIM and calponin homology domains-containing protein 1 isoform X21
NM_001330791.1	NP_001317720.1	LIM and calponin homology domains-containing protein 1 isoform p
NM_014988.4	NP_055803.2	LIM and calponin homology domains-containing protein 1 isoform a
NM_001330789.1	NP_001317718.1	LIM and calponin homology domains-containing protein 1 isoform n
XM_011513658.2	XP_011511960.1	LIM and calponin homology domains-containing protein 1 isoform X35
NM_001289122.2	NP_001276051.1	LIM and calponin homology domains-containing protein 1 isoform f
NM_001330792.1	NP_001317721.1	LIM and calponin homology domains-containing protein 1 isoform q
NM_001112719.3	NP_001106190.1	LIM and calponin homology domains-containing protein 1 isoform d
NM_001112717.4	NP_001106188.1	LIM and calponin homology domains-containing protein 1 isoform b
NM_001330792.2	NP_001317721.1	LIM and calponin homology domains-containing protein 1 isoform q
NM_001112720.3	NP_001106191.1	LIM and calponin homology domains-containing protein 1 isoform e
NM_001112718.4	NP_001106189.1	LIM and calponin homology domains-containing protein 1 isoform c
NM_001289124.2	NP_001276053.1	LIM and calponin homology domains-containing protein 1 isoform g
NM_001289122.3	NP_001276051.1	LIM and calponin homology domains-containing protein 1 isoform f
XM_011513643.1	XP_011511945.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_005248058.1	XP_005248115.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_006713996.1	XP_006714059.1	LIM and calponin homology domains-containing protein 1 isoform X12
XM_017007896.1	XP_016863385.1	LIM and calponin homology domains-containing protein 1 isoform X13
XM_011513648.1	XP_011511950.1	LIM and calponin homology domains-containing protein 1 isoform X14
XM_024453938.1	XP_024309706.1	LIM and calponin homology domains-containing protein 1 isoform X25

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1
Homo sapiens (human)	LIMCH1	NP_055803.2

Gene Ontology
Bibliography

Related articles in PubMed

LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration.
Lin YH, Zhen YY, Chien KY, Lee IC, Lin WC, Chen MY, Pai LM
Molecular biology of the cell28(8)1054-1065(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that LIMCH1 plays a positive role in regulation of NM-II activity through effects on MRLC during cell migration.
Title: LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06760	NM_014988.5 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$544.50-$762.30 $1089.00
OHu06341	NM_001112719.3 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$636.30 $909.00
OHu06342	NM_001112720.3 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$629.30 $899.00
OHu06603	NM_001289124.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu65265	NM_001330983.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu65266	NM_001330674.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu65264	XM_011513655.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu65267	XM_011513658.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu100276	NM_001330784.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100278	NM_001330982.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100281	NM_001330793.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100284	NM_001330786.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu105497	XM_024453938.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu94982	XM_017007903.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100274	NM_001330789.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100280	NM_001330788.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100283	NM_001330787.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu105496	XM_024453937.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94980	XM_017007898.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94981	XM_017007900.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu06336	NM_001112717.4 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu06340	NM_001112718.4 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu06600	NM_001289122.3 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38534	NM_001330790.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38536	NM_001330791.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38537	NM_001330792.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65262	XM_011513653.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65263	XM_011513654.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu94976	XM_017007894.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94977	XM_017007895.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94978	XM_017007896.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94979	XM_017007897.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu38528	NM_001330672.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu38518	XM_005248057.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38519	XM_005248058.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38521	XM_005248060.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38522	XM_005248061.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38524	XM_006713996.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65253	XM_011513642.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65254	XM_011513643.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65255	XM_011513644.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65256	XM_011513645.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65257	XM_011513646.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65258	XM_011513648.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65259	XM_011513649.1 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu65260	XM_011513651.2 Latest version!	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu100276	NM_001330784.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100280	NM_001330788.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100283	NM_001330787.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100284	NM_001330786.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu06760	NM_014988.3	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$544.50-$762.30 $1089.00
OHu38528	NM_001330672.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu38534	NM_001330790.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38536	NM_001330791.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu06760	NM_014988.4	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$544.50-$762.30 $1089.00
OHu100274	NM_001330789.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu06336	NM_001112717.3	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38537	NM_001330792.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu06340	NM_001112718.3	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu06603	NM_001289124.1	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu06600	NM_001289122.2	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMCH1 ( NM_014988.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055803.2 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

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CloneID	OHu06760
Clone ID Related Accession (Same CDS sequence)	NM_014988.3 , NM_014988.5 , NM_014988.4
Accession Version	NM_014988.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	LIM and calponin homology domains-containing protein 1 isoform a
Comment

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_055803.2

Translation

MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_014988.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014988.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_014988.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055803.2 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

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CloneID	OHu06760
Clone ID Related Accession (Same CDS sequence)	NM_014988.3 , NM_014988.5 , NM_014988.4
Accession Version	NM_014988.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On Jun 1, 2019 this sequence version replaced NM_014988.4. Transcript Variant: This variant (1) encodes isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.165127.1, SRR1803615.110090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055803.2
CDS	35..3286
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_014988.5
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014988.5

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_014988.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055803.2 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06760
Clone ID Related Accession (Same CDS sequence)	NM_014988.3 , NM_014988.5 , NM_014988.4
Accession Version	NM_014988.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_014988.3. Transcript Variant: This variant (1) encodes isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.165127.1, AB029025.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055803.2
CDS	211..3462
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_014988.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014988.4

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112719.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106190.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu06341
Clone ID Related Accession (Same CDS sequence)	NM_001112719.3
Accession Version	NM_001112719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, CR749205.1, CR936661.1, AC105389.3, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112719.2. Transcript Variant: This variant (4) differs in the 5' UTR and coding region and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: CR749205.1, SRR1660807.249952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106190.1
CDS	367..3075
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112719.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112719.3

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112720.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106191.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 5, mRNA.

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CloneID	OHu06342
Clone ID Related Accession (Same CDS sequence)	NM_001112720.3
Accession Version	NM_001112720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, DA786971.1, BX537916.1, AC105389.3, CR936664.1, CR749205.1 and AK026815.1. On Sep 3, 2016 this sequence version replaced NM_001112720.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding region, uses an alternate in-frame splice site, and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (e) has a shorter and distinct N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BX537916.1, AK299297.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106191.1
CDS	367..3039
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112720.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112720.3

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001289124.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276053.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu06603
Clone ID Related Accession (Same CDS sequence)	NM_001289124.1 , NM_001289124.2
Accession Version	NM_001289124.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform g
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936610.1, BX537916.1, CR936601.1, CR749205.1 and AK026815.1. ##Evidence-Data-START## Transcript exon combination :: CR936610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276053.1
CDS	579..3311
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001289124.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289124.1

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001289124.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276053.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

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CloneID	OHu06603
Clone ID Related Accession (Same CDS sequence)	NM_001289124.1 , NM_001289124.2
Accession Version	NM_001289124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform g
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936610.1, BX537916.1, CR936601.1, CR749205.1 and AK026815.1. On May 31, 2019 this sequence version replaced NM_001289124.1. ##Evidence-Data-START## Transcript exon combination :: CR936610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276053.1
CDS	579..3311
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001289124.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289124.2

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGA GTCGCCTGGC 
ACTGCCAGTG TTCCGCTTAG AGTTCAGAAC TCTTGGCGAC GATCCCAGTT TTTCTCCCAG 
TCAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330983.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317912.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 20, mRNA.

Price & Availability↑

CloneID	OHu65265
Clone ID Related Accession (Same CDS sequence)	NM_001330983.1
Accession Version	NM_001330983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform t
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_011513656.1. Transcript Variant: This variant (20) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.104390.1, SRR1660809.31958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317912.1
CDS	174..2969
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330983.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330674.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317603.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 9, mRNA.

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CloneID	OHu65266
Clone ID Related Accession (Same CDS sequence)	NM_001330674.1
Accession Version	NM_001330674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform i
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294774.1, AK299835.1, CR936658.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 1, 2016 this sequence version replaced XM_011513657.1. ##Evidence-Data-START## Transcript exon combination :: AK294774.1, AK299835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317603.1
CDS	367..3117
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330674.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330674.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGTGGATT CTCCAAGCAG TGAGAAGTCA CCTGTTATGA CACCTTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513655.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511957.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu65264
Clone ID Related Accession (Same CDS sequence)	XM_011513655.1
Accession Version	XM_011513655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TATGACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511957.1
CDS	178..3015
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513655.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513655.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGGAGCACC 
AGCATGTTTG ACATGCGGTG TGAGGAGGAG GCCGCGGTGC AGCCGCACAG CAGGGCCCGC 
CAGGAGCAGC TGCAGCTGAT AAATAACCAG CTGAGGGAAG AGGACGACAA ATGGCAAGAT 
GACCTGGCTC GTTGGAAGAG TCGTAGAAGA AGTGTTTCTC AGGACTTAAT CAAGAAAGAG 
GAAGAAAGGA AAAAAATGGA GAAGTTACTG GCTGGAGAAG ATGGGACAAG TGAACGAAGG 
AAAAGCATCA AAACCTACAG AGAAATTGTT CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG 
CATGAAGCAT ATAAGAACGC TCGGTCCCAG GAGGAGGCAG AGGGGATCCT TCAACAGTAC 
ATTGAGAGGT TCACCATCAG TGAGGCTGTT CTCGAACGCT TGGAGATGCC AAAAATTCTG 
GAAAGAAGCC ATTCAACAGA GCCAAATTTA TCCTCCTTCC TGAATGACCC CAATCCCATG 
AAATACCTGC GGCAACAGTC ACTGCCTCCA CCCAAATTCA CTGCCACTGT TGAAACCACC 
ATTGCTCGTG CCAGTGTTCT GGATACCAGC ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA 
ACTGTCACTC CCAAAGCAGT GCCTATGCTG ACACCCAAGC CTTACTCCCA GCCCAAAAAT 
TCTCAAGATG TTCTGAAGAC CTTTAAGGTA GACGGGAAAG TCAGTGTGAA TGGAGAGACG 
GTTCATAGAG AGGAGGAGAA GGAAAGAGAG TGTCCCACGG TGGCACCTGC CCACTCCTTA 
ACCAAATCCC AGATGTTTGA AGGTGTGGCC AGAGTGCACG GGTCTCCACT GGAGCTGAAA 
CAAGACAACG GTAGCATCGA GATCAACATA AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC 
GCAGCAACCA CTGAGAAAAC GGAACCGAAT AGTCAAGAGG ACAAGAATGA TGGTGGAAAA 
TCAAGAAAAG GGAATATAGA ACTTGCCTCA TCAGAACCAC AGCATTTTAC AACAACTGTG 
ACTCGATGCA GCCCGACCGT GGCCTTTGTG GAATTTCCCT CCAGCCCCCA GCTGAAGAAT 
GATGTGTCGG AAGAAAAAGA CCAGAAGAAA CCAGAAAATG AAATGAGTGG AAAGGTGGAG 
TTGGTGCTGT CACAAAAGGT GGTAAAGCCA AAATCTCCAG AACCCGAAGC AACGCTGACA 
TTTCCATTTC TGGACAAAAT GCCTGAAGCC AACCAACTAC ATTTGCCAAA TCTCAATTCT 
CAAGAGTCGC CTGGCACTGC CAGTGTTCCG CTTAGAGTTC AGAACTCTTG GCGACGATCC 
CAGTTTTTCT CCCAGTCAGT GGATTCTCCA AGCAGTGAGA AGTCACCTGT TATGACACCT 
CAGTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513658.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511960.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X35, mRNA.

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CloneID	OHu65267
Clone ID Related Accession (Same CDS sequence)	XM_011513658.2
Accession Version	XM_011513658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511960.1
CDS	795..3452
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513658.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513658.2

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGGA GCACCAGCAT GTTTGACATG 
CGGTGTGAGG AGGAGGCCGC GGTGCAGCCG CACAGCAGGG CCCGCCAGGA GCAGCTGCAG 
CTGATAAATA ACCAGCTGAG GGAAGAGGAC GACAAATGGC AAGATGACCT GGCTCGTTGG 
AAGAGTCGTA GAAGAAGTGT TTCTCAGGAC TTAATCAAGA AAGAGGAAGA AAGGAAAAAA 
ATGGAGAAGT TACTGGCTGG AGAAGATGGG ACAAGTGAAC GAAGGAAAAG CATCAAAACC 
TACAGAGAAA TTGTTCAAGA AAAAGAGCGG AGAGAGAGAG AGCTGCATGA AGCATATAAG 
AACGCTCGGT CCCAGGAGGA GGCAGAGGGG ATCCTTCAAC AGTACATTGA GAGGTTCACC 
ATCAGTGAGG CTGTTCTCGA ACGCTTGGAG ATGCCAAAAA TTCTGGAAAG AAGCCATTCA 
ACAGAGCCAA ATTTATCCTC CTTCCTGAAT GACCCCAATC CCATGAAATA CCTGCGGCAA 
CAGTCACTGC CTCCACCCAA ATTCACTGCC ACTGTTGAAA CCACCATTGC TCGTGCCAGT 
GTTCTGGATA CCAGCATGTC AGCAGGCAGT GGGTCTCCAA GCAAAACTGT CACTCCCAAA 
GCAGTGCCTA TGCTGACACC CAAGCCTTAC TCCCAGCCCA AAAATTCTCA AGATGTTCTG 
AAGACCTTTA AGGTAGACGG GAAAGTCAGT GTGAATGGAG AGACGGTTCA TAGAGAGGAG 
GAGAAGGAAA GAGAGTGTCC CACGGTGGCA CCTGCCCACT CCTTAACCAA ATCCCAGATG 
TTTGAAGGTG TGGCCAGAGT GCACGGGTCT CCACTGGAGC TGAAACAAGA CAACGGTAGC 
ATCGAGATCA ACATAAAGAA GCCAAACTCT GTTCCCCAAG AGCTCGCAGC AACCACTGAG 
AAAACGGAAC CGAATAGTCA AGAGGACAAG AATGATGGTG GAAAATCAAG AAAAGGGAAT 
ATAGAACTTG CCTCATCAGA ACCACAGCAT TTTACAACAA CTGTGACTCG ATGCAGCCCG 
ACCGTGGCCT TTGTGGAATT TCCCTCCAGC CCCCAGCTGA AGAATGATGT GTCGGAAGAA 
AAAGACCAGA AGAAACCAGA AAATGAAATG AGTGGAAAGG TGGAGTTGGT GCTGTCACAA 
AAGGTGGTAA AGCCAAAATC TCCAGAACCC GAAGCAACGC TGACATTTCC ATTTCTGGAC 
AAAATGCCTG AAGCCAACCA ACTACATTTG CCAAATCTCA ATTCTCAAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330784.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317713.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu100276
Clone ID Related Accession (Same CDS sequence)	NM_001330784.2 , NM_001330784.1
Accession Version	NM_001330784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform j
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936658.1, AC108050.5, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001330784.1. ##Evidence-Data-START## Transcript exon combination :: CR936658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317713.1
CDS	35..2977
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330784.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330784.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317713.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu100276
Clone ID Related Accession (Same CDS sequence)	NM_001330784.2 , NM_001330784.1
Accession Version	NM_001330784.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform j
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, CR936658.1, AC108050.5, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. ##Evidence-Data-START## Transcript exon combination :: CR936658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317713.1
CDS	211..3153
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330784.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330982.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317911.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 19, mRNA.

Price & Availability↑

CloneID	OHu100278
Clone ID Related Accession (Same CDS sequence)	NM_001330982.1
Accession Version	NM_001330982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform s
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007905.1. Transcript Variant: This variant (19) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC 
AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA 
CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA 
ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA 
TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG 
GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA 
GTCCATGAAG ACCATCAGCT GGATACCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA 
CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA 
GATGTGAAGC CAAAAACCCT CCCGCTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC 
AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317911.1
CDS	367..3078
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330982.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TCAGTTTAAG TTCTGGGCAT GGGACCCAGA AGAGGAGCGC 
AGGCGACAGG AAAAATGGCA ACAGGAACAG GAACGTTTGC TCCAGGAGAG ATACCAGAAG 
GAGCAGGACA AGCTGAAAGA AGAGTGGGAA AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA 
CGCAGATACT ATGAGGAGGA GCGTAAGATA ATTGAAGACA CTGTGGTTCC ATTTACTGTT 
TCTTCAAGTT CCGCTGACCA GCTGTCTACC TCTTCCTCCA TGACTGAAGG CAGTGGGACA 
ATGAATAAGA TAGACCTGGG AAACTGTCAA GATGAAAAAC AAGACAGAAG ATGGAAGAAA 
TCATTCCAGG GAGATGACAG TGACTTATTG CTGAAGACTA GGGAAAGTGA TCGACTGGAG 
GAGAAGGGCA GCCTAACTGA AGGGGCCTTG GCTCATTCTG GGAACCCTGT ATCAAAAGGA 
GTCCATGAAG ACCATCAGCT GGATACCGAG GCTGGGGCCC CACACTGTGG AACAAACCCA 
CAGCTTGCTC AGGATCCATC CCAGAATCAG CAGACATCAA ATCCAACGCA CAGTTCAGAA 
GATGTGAAGC CAAAAACCCT CCCGCTGGAT AAAAGCATTA ACCATCAGAT CGAGTCTCCC 
AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG 
GGTAAAGGAG CTGCAATGAT CATCGAGACC CTCAATCTCT ATTTTCACAT CCAGTGTTTC 
AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA GATGCAGTGA GTGGGACGGA TGTTAGGATT 
CGAAATGGTC TCCTGAACTG TAATGATTGC TACATGCGAT CCAGAAGTGC CGGGCAGCCT 
ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330793.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317722.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 18, mRNA.

Price & Availability↑

CloneID	OHu100281
Clone ID Related Accession (Same CDS sequence)	NM_001330793.1
Accession Version	NM_001330793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform r
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007904.1. Transcript Variant: This variant (18) represents use of an alternate promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.58248.1, SRR1660807.73523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC 
TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA 
AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317722.1
CDS	367..3153
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330793.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330793.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TCTATAAGTG GAAAGAAGCT GTGCTCTTCC 
TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA ATGATCATCG AGACCCTCAA TCTCTATTTT 
CACATCCAGT GTTTCAGGTG TGGAATTTGT AAAGGCCAGC TTGGAGATGC AGTGAGTGGG 
ACGGATGTTA GGATTCGAAA TGGTCTCCTG AACTGTAATG ATTGCTACAT GCGATCCAGA 
AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330786.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317715.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu100284
Clone ID Related Accession (Same CDS sequence)	NM_001330786.2 , NM_001330786.1
Accession Version	NM_001330786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform k
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, BC023546.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001330786.1. Transcript Variant: This variant (11) represents use of an alternate promoter and initiates translation at a downstream start codon compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: BC023546.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG 
GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317715.1
CDS	404..3097
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330786.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330786.2

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG 
GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330786.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317715.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

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CloneID	OHu100284
Clone ID Related Accession (Same CDS sequence)	NM_001330786.2 , NM_001330786.1
Accession Version	NM_001330786.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform k
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, BC023546.2, AC105389.3, CR749205.1, AK026815.1 and BX537916.1. Transcript Variant: This variant (11) represents use of an alternate promoter and initiates translation at a downstream start codon compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: BC023546.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG 
GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317715.1
CDS	404..3097
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330786.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330786.1

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGAT ACGGTCCGAG AACTCCTGTG 
TCTGATGACG CAGAGAGCAC CAGCATGTTT GACATGCGGT GTGAGGAGGA GGCCGCGGTG 
CAGCCGCACA GCAGGGCCCG CCAGGAGCAG CTGCAGCTGA TAAATAACCA GCTGAGGGAA 
GAGGACGACA AATGGCAAGA TGACCTGGCT CGTTGGAAGA GTCGTAGAAG AAGTGTTTCT 
CAGGACTTAA TCAAGAAAGA GGAAGAAAGG AAAAAAATGG AGAAGTTACT GGCTGGAGAA 
GATGGGACAA GTGAACGAAG GAAAAGCATC AAAACCTACA GAGAAATTGT TCAAGAAAAA 
GAGCGGAGAG AGAGAGAGCT GCATGAAGCA TATAAGAACG CTCGGTCCCA GGAGGAGGCA 
GAGGGGATCC TTCAACAGTA CATTGAGAGG TTCACCATCA GTGAGGCTGT TCTCGAACGC 
TTGGAGATGC CAAAAATTCT GGAAAGAAGC CATTCAACAG AGCCAAATTT ATCCTCCTTC 
CTGAATGACC CCAATCCCAT GAAATACCTG CGGCAACAGT CACTGCCTCC ACCCAAATTC 
ACTGCCACTG TTGAAACCAC CATTGCTCGT GCCAGTGTTC TGGATACCAG CATGTCAGCA 
GGCAGTGGGT CTCCAAGCAA AACTGTCACT CCCAAAGCAG TGCCTATGCT GACACCCAAG 
CCTTACTCCC AGCCCAAAAA TTCTCAAGAT GTTCTGAAGA CCTTTAAGGT AGACGGGAAA 
GTCAGTGTGA ATGGAGAGAC GGTTCATAGA GAGGAGGAGA AGGAAAGAGA GTGTCCCACG 
GTGGCACCTG CCCACTCCTT AACCAAATCC CAGATGTTTG AAGGTGTGGC CAGAGTGCAC 
GGGTCTCCAC TGGAGCTGAA ACAAGACAAC GGTAGCATCG AGATCAACAT AAAGAAGCCA 
AACTCTGTTC CCCAAGAGCT CGCAGCAACC ACTGAGAAAA CGGAACCGAA TAGTCAAGAG 
GACAAGAATG ATGGTGGAAA ATCAAGAAAA GGGAATATAG AACTTGCCTC ATCAGAACCA 
CAGCATTTTA CAACAACTGT GACTCGATGC AGCCCGACCG TGGCCTTTGT GGAATTTCCC 
TCCAGCCCCC AGCTGAAGAA TGATGTGTCG GAAGAAAAAG ACCAGAAGAA ACCAGAAAAT 
GAAATGAGTG GAAAGGTGGA GTTGGTGCTG TCACAAAAGG TGGTAAAGCC AAAATCTCCA 
GAACCCGAAG CAACGCTGAC ATTTCCATTT CTGGACAAAA TGCCTGAAGC CAACCAACTA 
CATTTGCCAA ATCTCAATTC TCAAGTGGAT TCTCCAAGCA GTGAGAAGTC ACCTGTTATG 
ACACCTTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024453938.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309706.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

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CloneID	OHu105497
Clone ID Related Accession (Same CDS sequence)	XM_024453938.1
Accession Version	XM_024453938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TGAGAGATAC CAGAAGGAGC AGGACAAGCT GAAAGAAGAG 
TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG GAAGAACGCA GATACTATGA GGAGGAGCGT 
AAGATAATTG AAGACACTGT GGTTCCATTT ACTGTTTCTT CAAGTTCCGC TGACCAGCTG 
TCTACCTCTT CCTCCATGAC TGAAGGCAGT GGGACAATGA ATAAGATAGA CCTGGGAAAC 
TGTCAAGATG AAAAACAAGA CAGAAGATGG AAGAAATCAT TCCAGGGAGA TGACAGTGAC 
TTATTGCTGA AGACTAGGGA AAGTGATCGA CTGGAGGAGA AGGGCAGCCT AACTGAAGGG 
GCCTTGGCTC ATTCTGGGAA CCCTGTATCA AAAGGAGTCC ATGAAGACCA TCAGCTGGAT 
ACCGAGGCTG GGGCCCCACA CTGTGGAACA AACCCACAGC TTGCTCAGGA TCCATCCCAG 
AATCAGCAGA CATCAAATCC AACGCACAGT TCAGAAGATG TGAAGCCAAA AACCCTCCCG 
CTGGATAAAA GCATTAACCA TCAGATCGAG TCTCCCAGTG AAAGGCGGAA GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309706.1
CDS	178..2805
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024453938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453938.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG 
AAGTCACCTG TTATGACACC TGAGAGATAC CAGAAGGAGC AGGACAAGCT GAAAGAAGAG 
TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG GAAGAACGCA GATACTATGA GGAGGAGCGT 
AAGATAATTG AAGACACTGT GGTTCCATTT ACTGTTTCTT CAAGTTCCGC TGACCAGCTG 
TCTACCTCTT CCTCCATGAC TGAAGGCAGT GGGACAATGA ATAAGATAGA CCTGGGAAAC 
TGTCAAGATG AAAAACAAGA CAGAAGATGG AAGAAATCAT TCCAGGGAGA TGACAGTGAC 
TTATTGCTGA AGACTAGGGA AAGTGATCGA CTGGAGGAGA AGGGCAGCCT AACTGAAGGG 
GCCTTGGCTC ATTCTGGGAA CCCTGTATCA AAAGGAGTCC ATGAAGACCA TCAGCTGGAT 
ACCGAGGCTG GGGCCCCACA CTGTGGAACA AACCCACAGC TTGCTCAGGA TCCATCCCAG 
AATCAGCAGA CATCAAATCC AACGCACAGT TCAGAAGATG TGAAGCCAAA AACCCTCCCG 
CTGGATAAAA GCATTAACCA TCAGATCGAG TCTCCCAGTG AAAGGCGGAA GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007903.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863392.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

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CloneID	OHu94982
Clone ID Related Accession (Same CDS sequence)	XM_017007903.1
Accession Version	XM_017007903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGT 
GTTCCGCTTA GAGTTCAGAA CTCTTGGCGA CGATCCCAGT TTTTCTCCCA GTCAGTGGAT 
TCTCCAAGCA GTGAGAAGTC ACCTGTTATG ACACCTCAGT TTAAGTTCTG GGCATGGGAC 
CCAGAAGAGG AGCGCAGGCG ACAGGAAAAA TGGCAACAGG AACAGGAACG TTTGCTCCAG 
GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG 
GTGGAAGAGG AAGAACGCAG ATACTATGAG GAGGAGCGTA AGATAATTGA AGACACTGTG 
GTTCCATTTA CTGTTTCTTC AAGTTCCGCT GACCAGCTGT CTACCTCTTC CTCCATGACT 
GAAGGCAGTG GGACAATGAA TAAGATAGAC CTGGGAAACT GTCAAGATGA AAAACAAGAC 
AGAAGATGGA AGAAATCATT CCAGGGAGAT GACAGTGACT TATTGCTGAA GACTAGGGAA 
AGTGATCGAC TGGAGGAGAA GGGCAGCCTA ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC 
CCTGTATCAA AAGGAGTCCA TGAAGACCAT CAGCTGGATA CCGAGGCTGG GGCCCCACAC 
TGTGGAACAA ACCCACAGCT TGCTCAGGAT CCATCCCAGA ATCAGCAGAC ATCAAATCCA 
ACGCACAGTT CAGAAGATGT GAAGCCAAAA ACCCTCCCGC TGGATAAAAG CATTAACCAT 
CAGATCGAGT CTCCCAGTGA AAGGCGGAAG AAAAGTCCCC GAGAGCACTT CCAGGCTGGG 
CCTTTCTCTC CCTGTTCTCC CACCCCTCCC GGTCAGTCAC CAAACAGGTA CAAGAGGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863392.1
CDS	178..3051
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007903.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT 
CCGAGAACTC CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG 
GAGGAGGCCG CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT 
AACCAGCTGA GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT 
AGAAGAAGTG TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG 
TTACTGGCTG GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA 
ATTGTTCAAG AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG 
TCCCAGGAGG AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG 
GCTGTTCTCG AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA 
AATTTATCCT CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG 
CCTCCACCCA AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT 
ACCAGCATGT CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT 
AAGGTAGACG GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA 
AGAGAGTGTC CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT 
GTGGCCAGAG TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC 
AACATAAAGA AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA 
CCGAATAGTC AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT 
GCCTCATCAG AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC 
TTTGTGGAAT TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG 
AAGAAACCAG AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA 
AAGCCAAAAT CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT 
GAAGCCAACC AACTACATTT GCCAAATCTC AATTCTCAAG AGTCGCCTGG CACTGCCAGT 
GTTCCGCTTA GAGTTCAGAA CTCTTGGCGA CGATCCCAGT TTTTCTCCCA GTCAGTGGAT 
TCTCCAAGCA GTGAGAAGTC ACCTGTTATG ACACCTCAGT TTAAGTTCTG GGCATGGGAC 
CCAGAAGAGG AGCGCAGGCG ACAGGAAAAA TGGCAACAGG AACAGGAACG TTTGCTCCAG 
GAGAGATACC AGAAGGAGCA GGACAAGCTG AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG 
GTGGAAGAGG AAGAACGCAG ATACTATGAG GAGGAGCGTA AGATAATTGA AGACACTGTG 
GTTCCATTTA CTGTTTCTTC AAGTTCCGCT GACCAGCTGT CTACCTCTTC CTCCATGACT 
GAAGGCAGTG GGACAATGAA TAAGATAGAC CTGGGAAACT GTCAAGATGA AAAACAAGAC 
AGAAGATGGA AGAAATCATT CCAGGGAGAT GACAGTGACT TATTGCTGAA GACTAGGGAA 
AGTGATCGAC TGGAGGAGAA GGGCAGCCTA ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC 
CCTGTATCAA AAGGAGTCCA TGAAGACCAT CAGCTGGATA CCGAGGCTGG GGCCCCACAC 
TGTGGAACAA ACCCACAGCT TGCTCAGGAT CCATCCCAGA ATCAGCAGAC ATCAAATCCA 
ACGCACAGTT CAGAAGATGT GAAGCCAAAA ACCCTCCCGC TGGATAAAAG CATTAACCAT 
CAGATCGAGT CTCCCAGTGA AAGGCGGAAG AAAAGTCCCC GAGAGCACTT CCAGGCTGGG 
CCTTTCTCTC CCTGTTCTCC CACCCCTCCC GGTCAGTCAC CAAACAGGTA CAAGAGGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330789.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317718.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

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CloneID	OHu100274
Clone ID Related Accession (Same CDS sequence)	NM_001330789.2 , NM_001330789.1
Accession Version	NM_001330789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform n
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330789.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97937.1, SRR1803614.108979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317718.1
CDS	35..3124
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330789.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330789.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330789.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317718.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu100274
Clone ID Related Accession (Same CDS sequence)	NM_001330789.2 , NM_001330789.1
Accession Version	NM_001330789.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform n
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007902.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97937.1, SRR1660807.109176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317718.1
CDS	211..3300
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330789.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330789.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330788.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317717.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu100280
Clone ID Related Accession (Same CDS sequence)	NM_001330788.1 , NM_001330788.2
Accession Version	NM_001330788.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform m
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007901.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.237697.1, SRR1660803.88863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317717.1
CDS	211..3378
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330788.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330788.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330788.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317717.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu100280
Clone ID Related Accession (Same CDS sequence)	NM_001330788.1 , NM_001330788.2
Accession Version	NM_001330788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform m
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330788.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.156655.1, SRR1660803.88863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317717.1
CDS	35..3202
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330788.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330788.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317716.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu100283
Clone ID Related Accession (Same CDS sequence)	NM_001330787.2 , NM_001330787.1
Accession Version	NM_001330787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform l
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330787.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.214498.1, SRR1660803.230038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317716.1
CDS	35..3292
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330787.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330787.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317716.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

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CloneID	OHu100283
Clone ID Related Accession (Same CDS sequence)	NM_001330787.2 , NM_001330787.1
Accession Version	NM_001330787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform l
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_017007899.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.230038.1, SRR1803611.214498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317716.1
CDS	211..3468
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330787.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTACAAGAG GTCTATAAGT GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT 
CCTTTGGGTA AAGGAGCTGC AATGATCATC GAGACCCTCA ATCTCTATTT TCACATCCAG 
TGTTTCAGGT GTGGAATTTG TAAAGGCCAG CTTGGAGATG CAGTGAGTGG GACGGATGTT 
AGGATTCGAA ATGGTCTCCT GAACTGTAAT GATTGCTACA TGCGATCCAG AAGTGCCGGG 
CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_024453937.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309705.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu105496
Clone ID Related Accession (Same CDS sequence)	XM_024453937.1
Accession Version	XM_024453937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309705.1
CDS	57..3281
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_024453937.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453937.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007898.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863387.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

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CloneID	OHu94980
Clone ID Related Accession (Same CDS sequence)	XM_017007898.1
Accession Version	XM_017007898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863387.1
CDS	57..3317
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007898.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007898.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA 
AGTCCCCGAG AGCACTTCCA GGCTGGGCCT TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT 
CAGTCACCAA ACAGGTACAA GAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007900.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863389.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

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CloneID	OHu94981
Clone ID Related Accession (Same CDS sequence)	XM_017007900.1
Accession Version	XM_017007900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTACAAGAGG 
TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA 
ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT 
AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG 
AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863389.1
CDS	57..3233
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007900.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGAAAAGTC CCCGAGAGCA CTTCCAGGCT 
GGGCCTTTCT CTCCCTGTTC TCCCACCCCT CCCGGTCAGT CACCAAACAG GTACAAGAGG 
TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA 
ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT 
AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG 
AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112717.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106188.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06336
Clone ID Related Accession (Same CDS sequence)	NM_001112717.3 , NM_001112717.4
Accession Version	NM_001112717.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR933645.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112717.2. ##Evidence-Data-START## Transcript exon combination :: CR933645.1, SRR1803612.87314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106188.1
CDS	211..3384
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112717.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112717.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112717.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106188.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06336
Clone ID Related Accession (Same CDS sequence)	NM_001112717.3 , NM_001112717.4
Accession Version	NM_001112717.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR933645.1, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001112717.3. ##Evidence-Data-START## Transcript exon combination :: CR933645.1, SRR1803612.87314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106188.1
CDS	35..3208
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112717.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112717.4

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTCAGTTTA AGTTCTGGGC ATGGGACCCA GAAGAGGAGC GCAGGCGACA GGAAAAATGG 
CAACAGGAAC AGGAACGTTT GCTCCAGGAG AGATACCAGA AGGAGCAGGA CAAGCTGAAA 
GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG GAAGAGGAAG AACGCAGATA CTATGAGGAG 
GAGCGTAAGA TAATTGAAGA CACTGTGGTT CCATTTACTG TTTCTTCAAG TTCCGCTGAC 
CAGCTGTCTA CCTCTTCCTC CATGACTGAA GGCAGTGGGA CAATGAATAA GATAGACCTG 
GGAAACTGTC AAGATGAAAA ACAAGACAGA AGATGGAAGA AATCATTCCA GGGAGATGAC 
AGTGACTTAT TGCTGAAGAC TAGGGAAAGT GATCGACTGG AGGAGAAGGG CAGCCTAACT 
GAAGGGGCCT TGGCTCATTC TGGGAACCCT GTATCAAAAG GAGTCCATGA AGACCATCAG 
CTGGATACCG AGGCTGGGGC CCCACACTGT GGAACAAACC CACAGCTTGC TCAGGATCCA 
TCCCAGAATC AGCAGACATC AAATCCAACG CACAGTTCAG AAGATGTGAA GCCAAAAACC 
CTCCCGCTGG ATAAAAGCAT TAACCATCAG ATCGAGTCTC CCAGTGAAAG GCGGAAGTCT 
ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT GGGCTTCCTT TGGGTAAAGG AGCTGCAATG 
ATCATCGAGA CCCTCAATCT CTATTTTCAC ATCCAGTGTT TCAGGTGTGG AATTTGTAAA 
GGCCAGCTTG GAGATGCAGT GAGTGGGACG GATGTTAGGA TTCGAAATGG TCTCCTGAAC 
TGTAATGATT GCTACATGCG ATCCAGAAGT GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112718.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106189.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06340
Clone ID Related Accession (Same CDS sequence)	NM_001112718.3 , NM_001112718.4
Accession Version	NM_001112718.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AB029025.2, AC105389.3, CR936664.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001112718.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.22792.1, SRR1803616.158804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106189.1
CDS	211..3381
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112718.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112718.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001112718.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106189.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

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CloneID	OHu06340
Clone ID Related Accession (Same CDS sequence)	NM_001112718.3 , NM_001112718.4
Accession Version	NM_001112718.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB029025.2, AC105389.3, CR936664.1, CR749205.1, AK026815.1 and BX537916.1. On May 31, 2019 this sequence version replaced NM_001112718.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.158804.1, SRR1803617.199715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106189.1
CDS	35..3205
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001112718.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112718.4

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001289122.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276051.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu06600
Clone ID Related Accession (Same CDS sequence)	NM_001289122.2 , NM_001289122.3
Accession Version	NM_001289122.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform f
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, CR936658.1, AB029025.2, CR936601.1, CR749205.1, AK026815.1 and BX537916.1. On Sep 3, 2016 this sequence version replaced NM_001289122.1. ##Evidence-Data-START## Transcript exon combination :: CR936601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276051.1
CDS	211..3420
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001289122.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289122.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001289122.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276051.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

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CloneID	OHu06600
Clone ID Related Accession (Same CDS sequence)	NM_001289122.2 , NM_001289122.3
Accession Version	NM_001289122.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform f
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR936658.1, AB029025.2, CR936601.1, CR749205.1, AK026815.1 and BX537916.1. On Jun 1, 2019 this sequence version replaced NM_001289122.2. ##Evidence-Data-START## Transcript exon combination :: CR936601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276051.1
CDS	35..3244
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001289122.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289122.3

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTTTTAAGTT CTGGGCATGG 
GACCCAGAAG AGGAGCGCAG GCGACAGGAA AAATGGCAAC AGGAACAGGA ACGTTTGCTC 
CAGGAGAGAT ACCAGAAGGA GCAGGACAAG CTGAAAGAAG AGTGGGAAAA GGCCCAAAAG 
GAGGTGGAAG AGGAAGAACG CAGATACTAT GAGGAGGAGC GTAAGATAAT TGAAGACACT 
GTGGTTCCAT TTACTGTTTC TTCAAGTTCC GCTGACCAGC TGTCTACCTC TTCCTCCATG 
ACTGAAGGCA GTGGGACAAT GAATAAGATA GACCTGGGAA ACTGTCAAGA TGAAAAACAA 
GACAGAAGAT GGAAGAAATC ATTCCAGGGA GATGACAGTG ACTTATTGCT GAAGACTAGG 
GAAAGTGATC GACTGGAGGA GAAGGGCAGC CTAACTGAAG GGGCCTTGGC TCATTCTGGG 
AACCCTGTAT CAAAAGGAGT CCATGAAGAC CATCAGCTGG ATACCGAGGC TGGGGCCCCA 
CACTGTGGAA CAAACCCACA GCTTGCTCAG GATCCATCCC AGAATCAGCA GACATCAAAT 
CCAACGCACA GTTCAGAAGA TGTGAAGCCA AAAACCCTCC CGCTGGATAA AAGCATTAAC 
CATCAGATCG AGTCTCCCAG TGAAAGGCGG AAGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330790.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317719.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

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CloneID	OHu38534
Clone ID Related Accession (Same CDS sequence)	NM_001330790.1 , NM_001330790.2
Accession Version	NM_001330790.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform o
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248072.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.261334.1, SRR1803617.15300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317719.1
CDS	211..3459
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330790.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330790.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330790.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317719.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

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CloneID	OHu38534
Clone ID Related Accession (Same CDS sequence)	NM_001330790.1 , NM_001330790.2
Accession Version	NM_001330790.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform o
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5, AC124009.4 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330790.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.61307.1, SRR1803615.136930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317719.1
CDS	35..3283
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330790.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330790.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGTGGATTCT CCAAGCAGTG AGAAGTCACC TGTTATGACA 
CCTTTTAAGT TCTGGGCATG GGACCCAGAA GAGGAGCGCA GGCGACAGGA AAAATGGCAA 
CAGGAACAGG AACGTTTGCT CCAGGAGAGA TACCAGAAGG AGCAGGACAA GCTGAAAGAA 
GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA GAGGAAGAAC GCAGATACTA TGAGGAGGAG 
CGTAAGATAA TTGAAGACAC TGTGGTTCCA TTTACTGTTT CTTCAAGTTC CGCTGACCAG 
CTGTCTACCT CTTCCTCCAT GACTGAAGGC AGTGGGACAA TGAATAAGAT AGACCTGGGA 
AACTGTCAAG ATGAAAAACA AGACAGAAGA TGGAAGAAAT CATTCCAGGG AGATGACAGT 
GACTTATTGC TGAAGACTAG GGAAAGTGAT CGACTGGAGG AGAAGGGCAG CCTAACTGAA 
GGGGCCTTGG CTCATTCTGG GAACCCTGTA TCAAAAGGAG TCCATGAAGA CCATCAGCTG 
GATACCGAGG CTGGGGCCCC ACACTGTGGA ACAAACCCAC AGCTTGCTCA GGATCCATCC 
CAGAATCAGC AGACATCAAA TCCAACGCAC AGTTCAGAAG ATGTGAAGCC AAAAACCCTC 
CCGCTGGATA AAAGCATTAA CCATCAGATC GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT 
CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC TCTCCCTGTT CTCCCACCCC TCCCGGTCAG 
TCACCAAACA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330791.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317720.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu38536
Clone ID Related Accession (Same CDS sequence)	NM_001330791.2 , NM_001330791.1
Accession Version	NM_001330791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform p
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On May 31, 2019 this sequence version replaced NM_001330791.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.155570.1, SRR1803613.97206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317720.1
CDS	35..3247
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330791.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330791.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330791.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317720.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu38536
Clone ID Related Accession (Same CDS sequence)	NM_001330791.2 , NM_001330791.1
Accession Version	NM_001330791.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform p
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248074.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.97206.1, SRR1660807.155570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317720.1
CDS	211..3423
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330791.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330791.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGAA AAGTCCCCGA GAGCACTTCC AGGCTGGGCC TTTCTCTCCC 
TGTTCTCCCA CCCCTCCCGG TCAGTCACCA AACAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330792.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317721.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu38537
Clone ID Related Accession (Same CDS sequence)	NM_001330792.1 , NM_001330792.2
Accession Version	NM_001330792.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform q
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Sep 3, 2016 this sequence version replaced XM_005248075.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.33203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317721.1
CDS	211..3345
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330792.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330792.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330792.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317721.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu38537
Clone ID Related Accession (Same CDS sequence)	NM_001330792.1 , NM_001330792.2
Accession Version	NM_001330792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform q
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095043.3, AC108050.5 and AC105389.3. On Jun 1, 2019 this sequence version replaced NM_001330792.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.33203.1, SRR7346977.1546087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317721.1
CDS	35..3169
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330792.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330792.2

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTTTT AAGTTCTGGG CATGGGACCC AGAAGAGGAG 
CGCAGGCGAC AGGAAAAATG GCAACAGGAA CAGGAACGTT TGCTCCAGGA GAGATACCAG 
AAGGAGCAGG ACAAGCTGAA AGAAGAGTGG GAAAAGGCCC AAAAGGAGGT GGAAGAGGAA 
GAACGCAGAT ACTATGAGGA GGAGCGTAAG ATAATTGAAG ACACTGTGGT TCCATTTACT 
GTTTCTTCAA GTTCCGCTGA CCAGCTGTCT ACCTCTTCCT CCATGACTGA AGGCAGTGGG 
ACAATGAATA AGATAGACCT GGGAAACTGT CAAGATGAAA AACAAGACAG AAGATGGAAG 
AAATCATTCC AGGGAGATGA CAGTGACTTA TTGCTGAAGA CTAGGGAAAG TGATCGACTG 
GAGGAGAAGG GCAGCCTAAC TGAAGGGGCC TTGGCTCATT CTGGGAACCC TGTATCAAAA 
GGAGTCCATG AAGACCATCA GCTGGATACC GAGGCTGGGG CCCCACACTG TGGAACAAAC 
CCACAGCTTG CTCAGGATCC ATCCCAGAAT CAGCAGACAT CAAATCCAAC GCACAGTTCA 
GAAGATGTGA AGCCAAAAAC CCTCCCGCTG GATAAAAGCA TTAACCATCA GATCGAGTCT 
CCCAGTGAAA GGCGGAAGTC TATAAGTGGA AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT 
TTGGGTAAAG GAGCTGCAAT GATCATCGAG ACCCTCAATC TCTATTTTCA CATCCAGTGT 
TTCAGGTGTG GAATTTGTAA AGGCCAGCTT GGAGATGCAG TGAGTGGGAC GGATGTTAGG 
ATTCGAAATG GTCTCCTGAA CTGTAATGAT TGCTACATGC GATCCAGAAG TGCCGGGCAG 
CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513653.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511955.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

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CloneID	OHu65262
Clone ID Related Accession (Same CDS sequence)	XM_011513653.1
Accession Version	XM_011513653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG 
AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511955.1
CDS	57..3392
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513653.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513653.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGATACG GTCCGAGAAC TCCTGTGTCT 
GATGACGCAG AGAGCACCAG CATGTTTGAC ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG 
CCGCACAGCA GGGCCCGCCA GGAGCAGCTG CAGCTGATAA ATAACCAGCT GAGGGAAGAG 
GACGACAAAT GGCAAGATGA CCTGGCTCGT TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG 
GACTTAATCA AGAAAGAGGA AGAAAGGAAA AAAATGGAGA AGTTACTGGC TGGAGAAGAT 
GGGACAAGTG AACGAAGGAA AAGCATCAAA ACCTACAGAG AAATTGTTCA AGAAAAAGAG 
CGGAGAGAGA GAGAGCTGCA TGAAGCATAT AAGAACGCTC GGTCCCAGGA GGAGGCAGAG 
GGGATCCTTC AACAGTACAT TGAGAGGTTC ACCATCAGTG AGGCTGTTCT CGAACGCTTG 
GAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAACAGAGC CAAATTTATC CTCCTTCCTG 
AATGACCCCA ATCCCATGAA ATACCTGCGG CAACAGTCAC TGCCTCCACC CAAATTCACT 
GCCACTGTTG AAACCACCAT TGCTCGTGCC AGTGTTCTGG ATACCAGCAT GTCAGCAGGC 
AGTGGGTCTC CAAGCAAAAC TGTCACTCCC AAAGCAGTGC CTATGCTGAC ACCCAAGCCT 
TACTCCCAGC CCAAAAATTC TCAAGATGTT CTGAAGACCT TTAAGGTAGA CGGGAAAGTC 
AGTGTGAATG GAGAGACGGT TCATAGAGAG GAGGAGAAGG AAAGAGAGTG TCCCACGGTG 
GCACCTGCCC ACTCCTTAAC CAAATCCCAG ATGTTTGAAG GTGTGGCCAG AGTGCACGGG 
TCTCCACTGG AGCTGAAACA AGACAACGGT AGCATCGAGA TCAACATAAA GAAGCCAAAC 
TCTGTTCCCC AAGAGCTCGC AGCAACCACT GAGAAAACGG AACCGAATAG TCAAGAGGAC 
AAGAATGATG GTGGAAAATC AAGAAAAGGG AATATAGAAC TTGCCTCATC AGAACCACAG 
CATTTTACAA CAACTGTGAC TCGATGCAGC CCGACCGTGG CCTTTGTGGA ATTTCCCTCC 
AGCCCCCAGC TGAAGAATGA TGTGTCGGAA GAAAAAGACC AGAAGAAACC AGAAAATGAA 
ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA 
CCCGAAGCAA CGCTGACATT TCCATTTCTG GACAAAATGC CTGAAGCCAA CCAACTACAT 
TTGCCAAATC TCAATTCTCA AGAGTCGCCT GGCACTGCCA GTGTTCCGCT TAGAGTTCAG 
AACTCTTGGC GACGATCCCA GTTTTTCTCC CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTCA GTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG 
CGACAGGAAA AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG 
CAGGACAAGC TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC 
AGATACTATG AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT 
TCAAGTTCCG CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG 
AATAAGATAG ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA 
TTCCAGGGAG ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG 
AAGGGCAGCC TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC 
CATGAAGACC ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG 
CTTGCTCAGG ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT 
GTGAAGCCAA AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT 
GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT 
CCCACCCCTC CCGGTCAGTC ACCAAACAGG TACAAGAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513654.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511956.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

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CloneID	OHu65263
Clone ID Related Accession (Same CDS sequence)	XM_011513654.1
Accession Version	XM_011513654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTCAGTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG 
AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT 
GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AGTGTTTCAG GTGTGGAATT 
TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT GGGACGGATG TTAGGATTCG AAATGGTCTC 
CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511956.1
CDS	57..3356
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513654.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513654.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGGAGCA CCAGCATGTT TGACATGCGG 
TGTGAGGAGG AGGCCGCGGT GCAGCCGCAC AGCAGGGCCC GCCAGGAGCA GCTGCAGCTG 
ATAAATAACC AGCTGAGGGA AGAGGACGAC AAATGGCAAG ATGACCTGGC TCGTTGGAAG 
AGTCGTAGAA GAAGTGTTTC TCAGGACTTA ATCAAGAAAG AGGAAGAAAG GAAAAAAATG 
GAGAAGTTAC TGGCTGGAGA AGATGGGACA AGTGAACGAA GGAAAAGCAT CAAAACCTAC 
AGAGAAATTG TTCAAGAAAA AGAGCGGAGA GAGAGAGAGC TGCATGAAGC ATATAAGAAC 
GCTCGGTCCC AGGAGGAGGC AGAGGGGATC CTTCAACAGT ACATTGAGAG GTTCACCATC 
AGTGAGGCTG TTCTCGAACG CTTGGAGATG CCAAAAATTC TGGAAAGAAG CCATTCAACA 
GAGCCAAATT TATCCTCCTT CCTGAATGAC CCCAATCCCA TGAAATACCT GCGGCAACAG 
TCACTGCCTC CACCCAAATT CACTGCCACT GTTGAAACCA CCATTGCTCG TGCCAGTGTT 
CTGGATACCA GCATGTCAGC AGGCAGTGGG TCTCCAAGCA AAACTGTCAC TCCCAAAGCA 
GTGCCTATGC TGACACCCAA GCCTTACTCC CAGCCCAAAA ATTCTCAAGA TGTTCTGAAG 
ACCTTTAAGG TAGACGGGAA AGTCAGTGTG AATGGAGAGA CGGTTCATAG AGAGGAGGAG 
AAGGAAAGAG AGTGTCCCAC GGTGGCACCT GCCCACTCCT TAACCAAATC CCAGATGTTT 
GAAGGTGTGG CCAGAGTGCA CGGGTCTCCA CTGGAGCTGA AACAAGACAA CGGTAGCATC 
GAGATCAACA TAAAGAAGCC AAACTCTGTT CCCCAAGAGC TCGCAGCAAC CACTGAGAAA 
ACGGAACCGA ATAGTCAAGA GGACAAGAAT GATGGTGGAA AATCAAGAAA AGGGAATATA 
GAACTTGCCT CATCAGAACC ACAGCATTTT ACAACAACTG TGACTCGATG CAGCCCGACC 
GTGGCCTTTG TGGAATTTCC CTCCAGCCCC CAGCTGAAGA ATGATGTGTC GGAAGAAAAA 
GACCAGAAGA AACCAGAAAA TGAAATGAGT GGAAAGGTGG AGTTGGTGCT GTCACAAAAG 
GTGGTAAAGC CAAAATCTCC AGAACCCGAA GCAACGCTGA CATTTCCATT TCTGGACAAA 
ATGCCTGAAG CCAACCAACT ACATTTGCCA AATCTCAATT CTCAAGAGTC GCCTGGCACT 
GCCAGTGTTC CGCTTAGAGT TCAGAACTCT TGGCGACGAT CCCAGTTTTT CTCCCAGTCA 
GTGGATTCTC CAAGCAGTGA GAAGTCACCT GTTATGACAC CTCAGTTTAA GTTCTGGGCA 
TGGGACCCAG AAGAGGAGCG CAGGCGACAG GAAAAATGGC AACAGGAACA GGAACGTTTG 
CTCCAGGAGA GATACCAGAA GGAGCAGGAC AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA 
AAGGAGGTGG AAGAGGAAGA ACGCAGATAC TATGAGGAGG AGCGTAAGAT AATTGAAGAC 
ACTGTGGTTC CATTTACTGT TTCTTCAAGT TCCGCTGACC AGCTGTCTAC CTCTTCCTCC 
ATGACTGAAG GCAGTGGGAC AATGAATAAG ATAGACCTGG GAAACTGTCA AGATGAAAAA 
CAAGACAGAA GATGGAAGAA ATCATTCCAG GGAGATGACA GTGACTTATT GCTGAAGACT 
AGGGAAAGTG ATCGACTGGA GGAGAAGGGC AGCCTAACTG AAGGGGCCTT GGCTCATTCT 
GGGAACCCTG TATCAAAAGG AGTCCATGAA GACCATCAGC TGGATACCGA GGCTGGGGCC 
CCACACTGTG GAACAAACCC ACAGCTTGCT CAGGATCCAT CCCAGAATCA GCAGACATCA 
AATCCAACGC ACAGTTCAGA AGATGTGAAG CCAAAAACCC TCCCGCTGGA TAAAAGCATT 
AACCATCAGA TCGAGTCTCC CAGTGAAAGG CGGAAGAAAA GTCCCCGAGA GCACTTCCAG 
GCTGGGCCTT TCTCTCCCTG TTCTCCCACC CCTCCCGGTC AGTCACCAAA CAGGTACAAG 
AGGTCTATAA GTGGAAAGAA GCTGTGCTCT TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT 
GCAATGATCA TCGAGACCCT CAATCTCTAT TTTCACATCC AGTGTTTCAG GTGTGGAATT 
TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT GGGACGGATG TTAGGATTCG AAATGGTCTC 
CTGAACTGTA ATGATTGCTA CATGCGATCC AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007894.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863383.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

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CloneID	OHu94976
Clone ID Related Accession (Same CDS sequence)	XM_017007894.1
Accession Version	XM_017007894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863383.1
CDS	57..5012
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007894.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007894.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT CTATAAGTGG AAAGAAGCTG 
TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA GGAGCTGCAA TGATCATCGA GACCCTCAAT 
CTCTATTTTC ACATCCAGTG TTTCAGGTGT GGAATTTGTA AAGGCCAGCT TGGAGATGCA 
GTGAGTGGGA CGGATGTTAG GATTCGAAAT GGTCTCCTGA ACTGTAATGA TTGCTACATG 
CGATCCAGAA GTGCCGGGCA GCCTACAACA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007895.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863384.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

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CloneID	OHu94977
Clone ID Related Accession (Same CDS sequence)	XM_017007895.1
Accession Version	XM_017007895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863384.1
CDS	57..4940
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007895.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007895.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007896.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863385.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

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CloneID	OHu94978
Clone ID Related Accession (Same CDS sequence)	XM_017007896.1
Accession Version	XM_017007896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT 
GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC 
CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT 
GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA 
ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT 
CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT 
CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA 
ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG 
TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA 
ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT 
AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG 
AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863385.1
CDS	57..4853
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007896.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007896.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT 
GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC 
CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT 
GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA 
ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT 
CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT 
CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA 
ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG 
TCTATAAGTG GAAAGAAGCT GTGCTCTTCC TGTGGGCTTC CTTTGGGTAA AGGAGCTGCA 
ATGATCATCG AGACCCTCAA TCTCTATTTT CACATCCAGT GTTTCAGGTG TGGAATTTGT 
AAAGGCCAGC TTGGAGATGC AGTGAGTGGG ACGGATGTTA GGATTCGAAA TGGTCTCCTG 
AACTGTAATG ATTGCTACAT GCGATCCAGA AGTGCCGGGC AGCCTACAAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_017007897.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863386.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu94979
Clone ID Related Accession (Same CDS sequence)	XM_017007897.1
Accession Version	XM_017007897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA 
ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC 
TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA 
GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG 
CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG 
GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG 
GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG 
CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT 
AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG 
AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG GGTAAAGGAG CTGCAATGAT CATCGAGACC 
CTCAATCTCT ATTTTCACAT CCAGTGTTTC AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA 
GATGCAGTGA GTGGGACGGA TGTTAGGATT CGAAATGGTC TCCTGAACTG TAATGATTGC 
TACATGCGAT CCAGAAGTGC CGGGCAGCCT ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863386.1
CDS	57..4778
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_017007897.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007897.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA 
ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC 
TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA 
GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG 
CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG 
GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG 
GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG 
CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT 
AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGTCTAT AAGTGGAAAG 
AAGCTGTGCT CTTCCTGTGG GCTTCCTTTG GGTAAAGGAG CTGCAATGAT CATCGAGACC 
CTCAATCTCT ATTTTCACAT CCAGTGTTTC AGGTGTGGAA TTTGTAAAGG CCAGCTTGGA 
GATGCAGTGA GTGGGACGGA TGTTAGGATT CGAAATGGTC TCCTGAACTG TAATGATTGC 
TACATGCGAT CCAGAAGTGC CGGGCAGCCT ACAACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330672.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317601.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu38528
Clone ID Related Accession (Same CDS sequence)	NM_001330672.1 , NM_001330672.2
Accession Version	NM_001330672.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-27
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform h
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, AL831962.2, AC108050.5, AC124009.4, AC105389.3, AB029025.2, CR749205.1, AK026815.1 and BX537916.1. On Sep 1, 2016 this sequence version replaced XM_005248067.2. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream start codon and multiple internal differences, including a large novel segment. The resulting isoform (h) has a longer overall length, but has a shorter N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL831962.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317601.1
CDS	435..4838
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330672.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330672.1

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( NM_001330672.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317601.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

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CloneID	OHu38528
Clone ID Related Accession (Same CDS sequence)	NM_001330672.1 , NM_001330672.2
Accession Version	NM_001330672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform h
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC325132.1, AL831962.2, AC108050.5, AC124009.4, AC105389.3, AB029025.2, CR749205.1, AK026815.1 and BX537916.1. On Nov 22, 2018 this sequence version replaced NM_001330672.1. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream start codon and multiple internal differences, including a large novel segment. The resulting isoform (h) has a longer overall length, but has a shorter N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL831962.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000503057.6/ ENSP00000425631.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317601.1
CDS	435..4838
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	NM_001330672.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330672.2

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGTGG ATTCTCCAAG CAGTGAGAAG 
TCACCTGTTA TGACACCTTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005248057.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248114.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OHu38518
Clone ID Related Accession (Same CDS sequence)	XM_005248057.1
Accession Version	XM_005248057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248114.1
CDS	57..4949
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005248057.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248057.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTCAGTT TAAGTTCTGG GCATGGGACC CAGAAGAGGA GCGCAGGCGA 
CAGGAAAAAT GGCAACAGGA ACAGGAACGT TTGCTCCAGG AGAGATACCA GAAGGAGCAG 
GACAAGCTGA AAGAAGAGTG GGAAAAGGCC CAAAAGGAGG TGGAAGAGGA AGAACGCAGA 
TACTATGAGG AGGAGCGTAA GATAATTGAA GACACTGTGG TTCCATTTAC TGTTTCTTCA 
AGTTCCGCTG ACCAGCTGTC TACCTCTTCC TCCATGACTG AAGGCAGTGG GACAATGAAT 
AAGATAGACC TGGGAAACTG TCAAGATGAA AAACAAGACA GAAGATGGAA GAAATCATTC 
CAGGGAGATG ACAGTGACTT ATTGCTGAAG ACTAGGGAAA GTGATCGACT GGAGGAGAAG 
GGCAGCCTAA CTGAAGGGGC CTTGGCTCAT TCTGGGAACC CTGTATCAAA AGGAGTCCAT 
GAAGACCATC AGCTGGATAC CGAGGCTGGG GCCCCACACT GTGGAACAAA CCCACAGCTT 
GCTCAGGATC CATCCCAGAA TCAGCAGACA TCAAATCCAA CGCACAGTTC AGAAGATGTG 
AAGCCAAAAA CCCTCCCGCT GGATAAAAGC ATTAACCATC AGATCGAGTC TCCCAGTGAA 
AGGCGGAAGA AAAGTCCCCG AGAGCACTTC CAGGCTGGGC CTTTCTCTCC CTGTTCTCCC 
ACCCCTCCCG GTCAGTCACC AAACAGGTAC AAGAGGTCTA TAAGTGGAAA GAAGCTGTGC 
TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA GCTGCAATGA TCATCGAGAC CCTCAATCTC 
TATTTTCACA TCCAGTGTTT CAGGTGTGGA ATTTGTAAAG GCCAGCTTGG AGATGCAGTG 
AGTGGGACGG ATGTTAGGAT TCGAAATGGT CTCCTGAACT GTAATGATTG CTACATGCGA 
TCCAGAAGTG CCGGGCAGCC TACAACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005248058.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248115.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OHu38519
Clone ID Related Accession (Same CDS sequence)	XM_005248058.1
Accession Version	XM_005248058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248115.1
CDS	57..4946
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005248058.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248058.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTACAAG AGGTCTATAA GTGGAAAGAA GCTGTGCTCT 
TCCTGTGGGC TTCCTTTGGG TAAAGGAGCT GCAATGATCA TCGAGACCCT CAATCTCTAT 
TTTCACATCC AGTGTTTCAG GTGTGGAATT TGTAAAGGCC AGCTTGGAGA TGCAGTGAGT 
GGGACGGATG TTAGGATTCG AAATGGTCTC CTGAACTGTA ATGATTGCTA CATGCGATCC 
AGAAGTGCCG GGCAGCCTAC AACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005248060.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248117.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu38521
Clone ID Related Accession (Same CDS sequence)	XM_005248060.1
Accession Version	XM_005248060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248117.1
CDS	57..4937
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005248060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248060.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGAAAA GTCCCCGAGA GCACTTCCAG GCTGGGCCTT TCTCTCCCTG TTCTCCCACC 
CCTCCCGGTC AGTCACCAAA CAGGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_005248061.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248118.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu38522
Clone ID Related Accession (Same CDS sequence)	XM_005248061.1
Accession Version	XM_005248061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA 
ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC 
TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA 
GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG 
CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG 
GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG 
GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG 
CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT 
AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGAAAAG TCCCCGAGAG 
CACTTCCAGG CTGGGCCTTT CTCTCCCTGT TCTCCCACCC CTCCCGGTCA GTCACCAAAC 
AGGTACAAGA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248118.1
CDS	57..4865
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_005248061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248061.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTGAGAG ATACCAGAAG GAGCAGGACA AGCTGAAAGA AGAGTGGGAA 
AAGGCCCAAA AGGAGGTGGA AGAGGAAGAA CGCAGATACT ATGAGGAGGA GCGTAAGATA 
ATTGAAGACA CTGTGGTTCC ATTTACTGTT TCTTCAAGTT CCGCTGACCA GCTGTCTACC 
TCTTCCTCCA TGACTGAAGG CAGTGGGACA ATGAATAAGA TAGACCTGGG AAACTGTCAA 
GATGAAAAAC AAGACAGAAG ATGGAAGAAA TCATTCCAGG GAGATGACAG TGACTTATTG 
CTGAAGACTA GGGAAAGTGA TCGACTGGAG GAGAAGGGCA GCCTAACTGA AGGGGCCTTG 
GCTCATTCTG GGAACCCTGT ATCAAAAGGA GTCCATGAAG ACCATCAGCT GGATACCGAG 
GCTGGGGCCC CACACTGTGG AACAAACCCA CAGCTTGCTC AGGATCCATC CCAGAATCAG 
CAGACATCAA ATCCAACGCA CAGTTCAGAA GATGTGAAGC CAAAAACCCT CCCGCTGGAT 
AAAAGCATTA ACCATCAGAT CGAGTCTCCC AGTGAAAGGC GGAAGAAAAG TCCCCGAGAG 
CACTTCCAGG CTGGGCCTTT CTCTCCCTGT TCTCCCACCC CTCCCGGTCA GTCACCAAAC 
AGGTACAAGA GGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT 
AAAGGAGCTG CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG 
TGTGGAATTT GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA 
AATGGTCTCC TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA 
ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_006713996.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714059.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

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CloneID	OHu38524
Clone ID Related Accession (Same CDS sequence)	XM_006713996.1
Accession Version	XM_006713996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714059.1
CDS	57..4859
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_006713996.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713996.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGTGGATT CTCCAAGCAG TGAGAAGTCA 
CCTGTTATGA CACCTTTTAA GTTCTGGGCA TGGGACCCAG AAGAGGAGCG CAGGCGACAG 
GAAAAATGGC AACAGGAACA GGAACGTTTG CTCCAGGAGA GATACCAGAA GGAGCAGGAC 
AAGCTGAAAG AAGAGTGGGA AAAGGCCCAA AAGGAGGTGG AAGAGGAAGA ACGCAGATAC 
TATGAGGAGG AGCGTAAGAT AATTGAAGAC ACTGTGGTTC CATTTACTGT TTCTTCAAGT 
TCCGCTGACC AGCTGTCTAC CTCTTCCTCC ATGACTGAAG GCAGTGGGAC AATGAATAAG 
ATAGACCTGG GAAACTGTCA AGATGAAAAA CAAGACAGAA GATGGAAGAA ATCATTCCAG 
GGAGATGACA GTGACTTATT GCTGAAGACT AGGGAAAGTG ATCGACTGGA GGAGAAGGGC 
AGCCTAACTG AAGGGGCCTT GGCTCATTCT GGGAACCCTG TATCAAAAGG AGTCCATGAA 
GACCATCAGC TGGATACCGA GGCTGGGGCC CCACACTGTG GAACAAACCC ACAGCTTGCT 
CAGGATCCAT CCCAGAATCA GCAGACATCA AATCCAACGC ACAGTTCAGA AGATGTGAAG 
CCAAAAACCC TCCCGCTGGA TAAAAGCATT AACCATCAGA TCGAGTCTCC CAGTGAAAGG 
CGGAAGTCTA TAAGTGGAAA GAAGCTGTGC TCTTCCTGTG GGCTTCCTTT GGGTAAAGGA 
GCTGCAATGA TCATCGAGAC CCTCAATCTC TATTTTCACA TCCAGTGTTT CAGGTGTGGA 
ATTTGTAAAG GCCAGCTTGG AGATGCAGTG AGTGGGACGG ATGTTAGGAT TCGAAATGGT 
CTCCTGAACT GTAATGATTG CTACATGCGA TCCAGAAGTG CCGGGCAGCC TACAACATTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513642.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511944.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

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CloneID	OHu65253
Clone ID Related Accession (Same CDS sequence)	XM_011513642.1
Accession Version	XM_011513642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511944.1
CDS	57..5024
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513642.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513642.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTACAAGAG GTCTATAAGT 
GGAAAGAAGC TGTGCTCTTC CTGTGGGCTT CCTTTGGGTA AAGGAGCTGC AATGATCATC 
GAGACCCTCA ATCTCTATTT TCACATCCAG TGTTTCAGGT GTGGAATTTG TAAAGGCCAG 
CTTGGAGATG CAGTGAGTGG GACGGATGTT AGGATTCGAA ATGGTCTCCT GAACTGTAAT 
GATTGCTACA TGCGATCCAG AAGTGCCGGG CAGCCTACAA CATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513643.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511945.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OHu65254
Clone ID Related Accession (Same CDS sequence)	XM_011513643.1
Accession Version	XM_011513643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511945.1
CDS	57..5021
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513643.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513644.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511946.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu65255
Clone ID Related Accession (Same CDS sequence)	XM_011513644.1
Accession Version	XM_011513644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA GTGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511946.1
CDS	57..5015
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513644.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513644.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGAAAAGT CCCCGAGAGC ACTTCCAGGC TGGGCCTTTC 
TCTCCCTGTT CTCCCACCCC TCCCGGTCAG TCACCAAACA GGTCTATAAG TGGAAAGAAG 
CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG CAATGATCAT CGAGACCCTC 
AATCTCTATT TTCACATCCA GTGTTTCAGG TGTGGAATTT GTAAAGGCCA GCTTGGAGAT 
GCAGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTCC TGAACTGTAA TGATTGCTAC 
ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513645.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511947.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu65256
Clone ID Related Accession (Same CDS sequence)	XM_011513645.1
Accession Version	XM_011513645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT 
GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC 
CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT 
GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA 
ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT 
CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT 
CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA 
ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG 
AAAAGTCCCC GAGAGCACTT CCAGGCTGGG CCTTTCTCTC CCTGTTCTCC CACCCCTCCC 
GGTCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511947.1
CDS	57..4940
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513645.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT GAGAGATACC AGAAGGAGCA GGACAAGCTG 
AAAGAAGAGT GGGAAAAGGC CCAAAAGGAG GTGGAAGAGG AAGAACGCAG ATACTATGAG 
GAGGAGCGTA AGATAATTGA AGACACTGTG GTTCCATTTA CTGTTTCTTC AAGTTCCGCT 
GACCAGCTGT CTACCTCTTC CTCCATGACT GAAGGCAGTG GGACAATGAA TAAGATAGAC 
CTGGGAAACT GTCAAGATGA AAAACAAGAC AGAAGATGGA AGAAATCATT CCAGGGAGAT 
GACAGTGACT TATTGCTGAA GACTAGGGAA AGTGATCGAC TGGAGGAGAA GGGCAGCCTA 
ACTGAAGGGG CCTTGGCTCA TTCTGGGAAC CCTGTATCAA AAGGAGTCCA TGAAGACCAT 
CAGCTGGATA CCGAGGCTGG GGCCCCACAC TGTGGAACAA ACCCACAGCT TGCTCAGGAT 
CCATCCCAGA ATCAGCAGAC ATCAAATCCA ACGCACAGTT CAGAAGATGT GAAGCCAAAA 
ACCCTCCCGC TGGATAAAAG CATTAACCAT CAGATCGAGT CTCCCAGTGA AAGGCGGAAG 
AAAAGTCCCC GAGAGCACTT CCAGGCTGGG CCTTTCTCTC CCTGTTCTCC CACCCCTCCC 
GGTCAGTCAC CAAACAGGTA CAAGAGGTCT ATAAGTGGAA AGAAGCTGTG CTCTTCCTGT 
GGGCTTCCTT TGGGTAAAGG AGCTGCAATG ATCATCGAGA CCCTCAATCT CTATTTTCAC 
ATCCAGTGTT TCAGGTGTGG AATTTGTAAA GGCCAGCTTG GAGATGCAGT GAGTGGGACG 
GATGTTAGGA TTCGAAATGG TCTCCTGAAC TGTAATGATT GCTACATGCG ATCCAGAAGT 
GCCGGGCAGC CTACAACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513646.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511948.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

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CloneID	OHu65257
Clone ID Related Accession (Same CDS sequence)	XM_011513646.1
Accession Version	XM_011513646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511948.1
CDS	57..4937
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513646.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513646.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGAAACC AGGGCCACAC AGAAGAGGTG AAGTTGATAG TGACCTGTAA CATGAGGGCT 
CAGGAAAGTG AACCTGTGGA AGGAGGACTC AGGAAGGTGC CAGATCTTCA CAAGGATGAC 
CTGGCCCAGC AGAGAATTCA GGGCAGCCTT GCCCCTCACC GCGAGCCCCC GAGCTTCATT 
ACGCTCTCCA ACATAACAGA AGCTGACTTG GAGACGTGGG AGAGACTCAA AGTGTCAGAA 
AAGGCGAGGG ATGGAGATGT TCAGCACATC TGTGCTTCTG AGCCTTCCCC AGAAATTAAA 
GCAGAAACTG CCATTCGTGA TGACTTTGCC AACCGCAAAG CAAGGGCCTC TAAGAAAGCT 
TCCAGCCCCA GGCAAAAGTT TGTGCACTTT GGGCCAGTGA CGGAGCTAGA TCAGCAGAAA 
TGGAAGCGGC TTAGCATTGG CAAGGCTGGG CCTAGAGAGG ATGAAGAAGA AGTCATCTGT 
CATGGCAGCA AGATTCAAAT GGACTCTGTG TCTCCTGTCT CAGCGGCCAC TTCCAGCTTA 
AAGGGCCACC AAATATTTAA TCGACAAAAT GACTGCAGAA CAATGAATTG TGGCCGAGGT 
GACTATTGCA GAAGGGCCTC GTGGCTGGCT CCTGTGCCGG AGTCTCAGGA AGAGTGGGTC 
TGCAGTTTGG GCGAGTGCCC GAGGGGGACA GAGGAAGTTA CATCCAAACA GCTGCCACAG 
GATGGCAAAG AAGAAACAGA AAGCGCTCCA AGAGATTCTG AGAGGCTGTC AAAGGCAGAA 
AGATCAGAGG ACAGCAGCCA GCCACTGGTG TGTCCTCTGG CCTCTGAGTG TGAGGCTTCA 
GGGACAGAAG AGAAGTTGGA GAAGATGACA GCTCCTGCCT GGAGTGGCAG TGGACTGAAA 
GGCCAGCGAA AGTTGGATGA TTCACGAAAA GATGACATGA TGGCCAGGAG AACTGGGATG 
TCCCTTAGAC ACACTGGATC CAACCCAAAC CAGTTCCTTC CAGTTCCATT TGCAAAGCAA 
CAGGATGTGG AAGAATCTTC TAAAGGTCTA CCCATGAAAG ATCAGAGATA CGGTCCGAGA 
ACTCCTGTGT CTGATGACGC AGAGAGCACC AGCATGTTTG ACATGCGGTG TGAGGAGGAG 
GCCGCGGTGC AGCCGCACAG CAGGGCCCGC CAGGAGCAGC TGCAGCTGAT AAATAACCAG 
CTGAGGGAAG AGGACGACAA ATGGCAAGAT GACCTGGCTC GTTGGAAGAG TCGTAGAAGA 
AGTGTTTCTC AGGACTTAAT CAAGAAAGAG GAAGAAAGGA AAAAAATGGA GAAGTTACTG 
GCTGGAGAAG ATGGGACAAG TGAACGAAGG AAAAGCATCA AAACCTACAG AGAAATTGTT 
CAAGAAAAAG AGCGGAGAGA GAGAGAGCTG CATGAAGCAT ATAAGAACGC TCGGTCCCAG 
GAGGAGGCAG AGGGGATCCT TCAACAGTAC ATTGAGAGGT TCACCATCAG TGAGGCTGTT 
CTCGAACGCT TGGAGATGCC AAAAATTCTG GAAAGAAGCC ATTCAACAGA GCCAAATTTA 
TCCTCCTTCC TGAATGACCC CAATCCCATG AAATACCTGC GGCAACAGTC ACTGCCTCCA 
CCCAAATTCA CTGCCACTGT TGAAACCACC ATTGCTCGTG CCAGTGTTCT GGATACCAGC 
ATGTCAGCAG GCAGTGGGTC TCCAAGCAAA ACTGTCACTC CCAAAGCAGT GCCTATGCTG 
ACACCCAAGC CTTACTCCCA GCCCAAAAAT TCTCAAGATG TTCTGAAGAC CTTTAAGGTA 
GACGGGAAAG TCAGTGTGAA TGGAGAGACG GTTCATAGAG AGGAGGAGAA GGAAAGAGAG 
TGTCCCACGG TGGCACCTGC CCACTCCTTA ACCAAATCCC AGATGTTTGA AGGTGTGGCC 
AGAGTGCACG GGTCTCCACT GGAGCTGAAA CAAGACAACG GTAGCATCGA GATCAACATA 
AAGAAGCCAA ACTCTGTTCC CCAAGAGCTC GCAGCAACCA CTGAGAAAAC GGAACCGAAT 
AGTCAAGAGG ACAAGAATGA TGGTGGAAAA TCAAGAAAAG GGAATATAGA ACTTGCCTCA 
TCAGAACCAC AGCATTTTAC AACAACTGTG ACTCGATGCA GCCCGACCGT GGCCTTTGTG 
GAATTTCCCT CCAGCCCCCA GCTGAAGAAT GATGTGTCGG AAGAAAAAGA CCAGAAGAAA 
CCAGAAAATG AAATGAGTGG AAAGGTGGAG TTGGTGCTGT CACAAAAGGT GGTAAAGCCA 
AAATCTCCAG AACCCGAAGC AACGCTGACA TTTCCATTTC TGGACAAAAT GCCTGAAGCC 
AACCAACTAC ATTTGCCAAA TCTCAATTCT CAAGAGTCGC CTGGCACTGC CAGTGTTCCG 
CTTAGAGTTC AGAACTCTTG GCGACGATCC CAGTTTTTCT CCCAGTCAGT GGATTCTCCA 
AGCAGTGAGA AGTCACCTGT TATGACACCT CAGTTTAAGT TCTGGGCATG GGACCCAGAA 
GAGGAGCGCA GGCGACAGGA AAAATGGCAA CAGGAACAGG AACGTTTGCT CCAGGAGAGA 
TACCAGAAGG AGCAGGACAA GCTGAAAGAA GAGTGGGAAA AGGCCCAAAA GGAGGTGGAA 
GAGGAAGAAC GCAGATACTA TGAGGAGGAG CGTAAGATAA TTGAAGACAC TGTGGTTCCA 
TTTACTGTTT CTTCAAGTTC CGCTGACCAG CTGTCTACCT CTTCCTCCAT GACTGAAGGC 
AGTGGGACAA TGAATAAGAT AGACCTGGGA AACTGTCAAG ATGAAAAACA AGACAGAAGA 
TGGAAGAAAT CATTCCAGGG AGATGACAGT GACTTATTGC TGAAGACTAG GGAAAGTGAT 
CGACTGGAGG AGAAGGGCAG CCTAACTGAA GGGGCCTTGG CTCATTCTGG GAACCCTGTA 
TCAAAAGGAG TCCATGAAGA CCATCAGCTG GATACCGAGG CTGGGGCCCC ACACTGTGGA 
ACAAACCCAC AGCTTGCTCA GGATCCATCC CAGAATCAGC AGACATCAAA TCCAACGCAC 
AGTTCAGAAG ATGTGAAGCC AAAAACCCTC CCGCTGGATA AAAGCATTAA CCATCAGATC 
GAGTCTCCCA GTGAAAGGCG GAAGTCTATA AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG 
CTTCCTTTGG GTAAAGGAGC TGCAATGATC ATCGAGACCC TCAATCTCTA TTTTCACATC 
CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC CAGCTTGGAG ATGCAGTGAG TGGGACGGAT 
GTTAGGATTC GAAATGGTCT CCTGAACTGT AATGATTGCT ACATGCGATC CAGAAGTGCC 
GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513648.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511950.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

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CloneID	OHu65258
Clone ID Related Accession (Same CDS sequence)	XM_011513648.1
Accession Version	XM_011513648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGCTAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAATGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTGTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAAACAGCT GCCACAGGAT 
GGCAAAGAAG AAACAGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGGTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGCTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTC 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAC 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGAGGACA AGAATGATGG TGGAAAATCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511950.1
CDS	57..4781
Translation	MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513648.1

ORF Insert Sequence:

ATGGCTTGTC CCGCTCTCGG TCTGGAAGCT CTTCAGCCCC TGCAGCCCGA GCCGCCCCCC 
GAGCCCGCCT TCTCCGAGGC GCAGAAGTGG ATTGAGCAAG TAACTGGCAG AAGTTTTGGT 
GATAAAGATT TTCGGACAGG TTTAGAAAAT GGAATCCTCC TCTGCGAGTT GCTGAATGCT 
ATAAAGCCAG GACTTGTTAA AAAGATCAAT AGATTGCCTA CCCCCATTGC AGGACTGGAC 
AATATTATCT TATTCTTGAG AGGTTGTAAA GAGCTCGGCC TTAAAGAATC TCAACTTTTT 
GACCCGAGTG ACCTCCAGGA TACATCCAAC AGAGTAACAG TCAAGAGCCT TGATTATAGT 
AGGAAGCTGA AAAATGTATT AGTTACCATT TACTGGCTGG GAAAAGCAGC AAACAGCTGC 
ACATCCTACA GCGGAACGAC ACTAAACCTG AAGGAGTTTG AAGGATTGTT GGCTCAGATG 
CGAAAGGACA CTGATGACAT TGAAAGTCCT AAACGCAGTA TCCGAGACAG TGGCTACATC 
GACTGCTGGG ATTCCGAGCG CAGCGACTCC CTCTCTCCTC CTCGCCACGG CAGAGATGAT 
TCCTTCGACA GCCTGGATTC CTTTGGCTCT CGCTCTCGGC AGACGCCTTC ACCAGATGTA 
GTCCTCAGGG GAAGCAGCGA TGGGAGAGGA AGCGACTCTG AATCCGACTT GCCTCATCGG 
AAGCTGCCAG ATGTGAAGAA GGATGACATG TCTGCACGGC GGACTTCCCA TGGTGAGCCG 
AAATCAGCAG TGCCTTTTAA CCAGTACCTC CCGAACAAAA GCAATCAGAC GGCCTACGTC 
CCCGCGCCTC TGAGAAAGAA GAAAGCAGAG AGAGAGGAAT ACCGCAAGAG CTGGAGTACC 
GCCACCTCCC CGCTGGGTGG GGAGAGGCCC TTCAGCTTTC CTGAAACGAT AGAGGAAGAG 
GGGAGTGAAG TGGGGTCTGC TGGTGAAGAC AACCCAGCTG GCCAAATGAA TCCTGGTTGG 
AAGCCTTCCG ATGGTGGGTG TGAATTACCT GACGGCAGTG GTAAGGAGCA CCCTTCTTCA 
GACGGGGCTG TGGTGGCACC AGCTCCCAAG TCTGAAGAAA AAGATGCTGC TGAGATCCAA 
AAGCGCAAAA GGCTAGAGCA AGCTGGAATC AAGGTCATGC CAGCAGCACA GCGCTTTGCC 
AGTCAAAAAC AATTAAGTGA AGAGAAAGAA GCTATTCGTG ATATTGTCCT TCGCAAAGAA 
AACTCTTTCC TGACCCACCA ACATGGCAAC GATTCAGAGG CAGAAGGTGA AGTTGTGTGT 
CGACTGCCTG ATCTTGAGAA GGATGACTTT GCTGCAAGGA GAGCAAGGAT GAACCAAACC 
AAGCCAATGG TGCCATTAAA TCAACTCCTC TATGGCCCTT ATCCAAAGAA AGGGGCAGAG 
AAATCAGATG GCAGCAAACA GCTCTCAAAG GGAATCTCAA AAAAAAGAAG TCTAGAATAT 
AAAAGGGATG GAGATGTTCA GCACATCTGT GCTTCTGAGC CTTCCCCAGA AATTAAAGCA 
GAAACTGCCA TTCGTGATGA CTTTGCCAAC CGCAAAGCAA GGGCCTCTAA GAAAGCTTCC 
AGCCCCAGGC AAAAGTTTGT GCACTTTGGG CCAGTGACGG AGCTAGATCA GCAGAAATGG 
AAGCGGCTTA GCATTGGCAA GGCTGGGCCT AGAGAGGATG AAGAAGAAGT CATCTGTCAT 
GGCAGCAAGA TTCAAATGGA CTCTGTGTCT CCTGTCTCAG CGGCCACTTC CAGCTTAAAG 
GGCCACCAAA TATTTAATCG ACAAAATGAC TGCAGAACAA TGAATTGTGG CCGAGGTGAC 
TATTGCAGAA GGGCCTCGTG GCTGGCTCCT GTGCCGGAGT CTCAGGAAGA GTGGGTCTGC 
AGTTTGGGCG AGTGCCCGAG GGGGACAGAG GAAGTTACAT CCAAACAGCT GCCACAGGAT 
GGCAAAGAAG AAACAGAAAG CGCTCCAAGA GATTCTGAGA GGCTGTCAAA GGCAGAAAGA 
TCAGAGGACA GCAGCCAGCC ACTGGTGTGT CCTCTGGCCT CTGAGTGTGA GGCTTCAGGG 
ACAGAAGAGA AGTTGGAGAA GATGACAGCT CCTGCCTGGA GTGGCAGTGG ACTGAAAGGC 
CAGCGAAAGT TGGATGATTC ACGAAAAGAT GACATGATGG CCAGGAGAAC TGGGATGTCC 
CTTAGACACA CTGGATCCAA CCCAAACCAG TTCCTTCCAG TTCCATTTGC AAAGCAACAG 
GATGTGGAAG AATCTTCTAA AGGTCTACCC ATGAAAGATC AGAGATACGG TCCGAGAACT 
CCTGTGTCTG ATGACGCAGA GAGCACCAGC ATGTTTGACA TGCGGTGTGA GGAGGAGGCC 
GCGGTGCAGC CGCACAGCAG GGCCCGCCAG GAGCAGCTGC AGCTGATAAA TAACCAGCTG 
AGGGAAGAGG ACGACAAATG GCAAGATGAC CTGGCTCGTT GGAAGAGTCG TAGAAGAAGT 
GTTTCTCAGG ACTTAATCAA GAAAGAGGAA GAAAGGAAAA AAATGGAGAA GTTACTGGCT 
GGAGAAGATG GGACAAGTGA ACGAAGGAAA AGCATCAAAA CCTACAGAGA AATTGTTCAA 
GAAAAAGAGC GGAGAGAGAG AGAGCTGCAT GAAGCATATA AGAACGCTCG GTCCCAGGAG 
GAGGCAGAGG GGATCCTTCA ACAGTACATT GAGAGGTTCA CCATCAGTGA GGCTGTTCTC 
GAACGCTTGG AGATGCCAAA AATTCTGGAA AGAAGCCATT CAACAGAGCC AAATTTATCC 
TCCTTCCTGA ATGACCCCAA TCCCATGAAA TACCTGCGGC AACAGTCACT GCCTCCACCC 
AAATTCACTG CCACTGTTGA AACCACCATT GCTCGTGCCA GTGTTCTGGA TACCAGCATG 
TCAGCAGGCA GTGGGTCTCC AAGCAAAACT GTCACTCCCA AAGCAGTGCC TATGCTGACA 
CCCAAGCCTT ACTCCCAGCC CAAAAATTCT CAAGATGTTC TGAAGACCTT TAAGGTAGAC 
GGGAAAGTCA GTGTGAATGG AGAGACGGTT CATAGAGAGG AGGAGAAGGA AAGAGAGTGT 
CCCACGGTGG CACCTGCCCA CTCCTTAACC AAATCCCAGA TGTTTGAAGG TGTGGCCAGA 
GTGCACGGGT CTCCACTGGA GCTGAAACAA GACAACGGTA GCATCGAGAT CAACATAAAG 
AAGCCAAACT CTGTTCCCCA AGAGCTCGCA GCAACCACTG AGAAAACGGA ACCGAATAGT 
CAAGAGGACA AGAATGATGG TGGAAAATCA AGAAAAGGGA ATATAGAACT TGCCTCATCA 
GAACCACAGC ATTTTACAAC AACTGTGACT CGATGCAGCC CGACCGTGGC CTTTGTGGAA 
TTTCCCTCCA GCCCCCAGCT GAAGAATGAT GTGTCGGAAG AAAAAGACCA GAAGAAACCA 
GAAAATGAAA TGAGTGGAAA GGTGGAGTTG GTGCTGTCAC AAAAGGTGGT AAAGCCAAAA 
TCTCCAGAAC CCGAAGCAAC GCTGACATTT CCATTTCTGG ACAAAATGCC TGAAGCCAAC 
CAACTACATT TGCCAAATCT CAATTCTCAA GAGTCGCCTG GCACTGCCAG TGTTCCGCTT 
AGAGTTCAGA ACTCTTGGCG ACGATCCCAG TTTTTCTCCC AGTCAGTGGA TTCTCCAAGC 
AGTGAGAAGT CACCTGTTAT GACACCTCAG TTTAAGTTCT GGGCATGGGA CCCAGAAGAG 
GAGCGCAGGC GACAGGAAAA ATGGCAACAG GAACAGGAAC GTTTGCTCCA GGAGAGATAC 
CAGAAGGAGC AGGACAAGCT GAAAGAAGAG TGGGAAAAGG CCCAAAAGGA GGTGGAAGAG 
GAAGAACGCA GATACTATGA GGAGGAGCGT AAGATAATTG AAGACACTGT GGTTCCATTT 
ACTGTTTCTT CAAGTTCCGC TGACCAGCTG TCTACCTCTT CCTCCATGAC TGAAGGCAGT 
GGGACAATGA ATAAGATAGA CCTGGGAAAC TGTCAAGATG AAAAACAAGA CAGAAGATGG 
AAGAAATCAT TCCAGGGAGA TGACAGTGAC TTATTGCTGA AGACTAGGGA AAGTGATCGA 
CTGGAGGAGA AGGGCAGCCT AACTGAAGGG GCCTTGGCTC ATTCTGGGAA CCCTGTATCA 
AAAGGAGTCC ATGAAGACCA TCAGCTGGAT ACCGAGGCTG GGGCCCCACA CTGTGGAACA 
AACCCACAGC TTGCTCAGGA TCCATCCCAG AATCAGCAGA CATCAAATCC AACGCACAGT 
TCAGAAGATG TGAAGCCAAA AACCCTCCCG CTGGATAAAA GCATTAACCA TCAGATCGAG 
TCTCCCAGTG AAAGGCGGAA GAAAAGTCCC CGAGAGCACT TCCAGGCTGG GCCTTTCTCT 
CCCTGTTCTC CCACCCCTCC CGGTCAGTCA CCAAACAGGT ACAAGAGGTC TATAAGTGGA 
AAGAAGCTGT GCTCTTCCTG TGGGCTTCCT TTGGGTAAAG GAGCTGCAAT GATCATCGAG 
ACCCTCAATC TCTATTTTCA CATCCAGTGT TTCAGGTGTG GAATTTGTAA AGGCCAGCTT 
GGAGATGCAG TGAGTGGGAC GGATGTTAGG ATTCGAAATG GTCTCCTGAA CTGTAATGAT 
TGCTACATGC GATCCAGAAG TGCCGGGCAG CCTACAACAT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513649.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511951.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

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CloneID	OHu65259
Clone ID Related Accession (Same CDS sequence)	XM_011513649.1
Accession Version	XM_011513649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGCTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCAG AGAATTCAGG GCAGCCTTGC CCCTCACCGC 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGCTAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAATGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTGTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAAACAGC TGCCACAGGA TGGCAAAGAA GAAACAGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGGTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGCTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA CGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGAGGAC AAGAATGATG GTGGAAAATC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA TGACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TACCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC GCTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAGTG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511951.1
CDS	178..4683
Translation	MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513649.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513649.1

ORF Insert Sequence:

ATGGATTCCG AAAGACAAGT CAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC 
CGAGACAGTG GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT 
CGCCACGGCA GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG 
ACGCCTTCAC CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA 
TCCGACTTGC CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG 
ACTTCCCATG GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC 
AATCAGACGG CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC 
CGCAAGAGCT GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGCTTTCCT 
GAAACGATAG AGGAAGAGGG GAGTGAAGTG GGGTCTGCTG GTGAAGACAA CCCAGCTGGC 
CAAATGAATC CTGGTTGGAA GCCTTCCGAT GGTGGGTGTG AATTACCTGA CGGCAGTGGT 
AAGGAGCACC CTTCTTCAGA CGGGGCTGTG GTGGCACCAG CTCCCAAGTC TGAAGAAAAA 
GATGCTGCTG AGATCCAAAA GCGCAAAAGG CTAGAGCAAG CTGGAATCAA GGTCATGCCA 
GCAGCACAGC GCTTTGCCAG TCAAAAACAA TTAAGTGAAG AGAAAGAAGC TATTCGTGAT 
ATTGTCCTTC GCAAAGAAAA CTCTTTCCTG ACCCACCAAC ATGGCAACGA TTCAGAGGCA 
GAAGGTGAAG TTGTGTGTCG ACTGCCTGAT CTTGAGAAGG ATGACTTTGC TGCAAGGAGA 
GCAAGGATGA ACCAAACCAA GCCAATGGTG CCATTAAATC AACTCCTCTA TGGCCCTTAT 
CCAAAGAAAG GGGCAGAGAA ATCAGATGGC AGCAAACAGC TCTCAAAGGG AATCTCAAAA 
AAAAGAAGTC TAGAATATAA AAGAAACCAG GGCCACACAG AAGAGGTGAA GTTGATAGTG 
ACCTGTAACA TGAGGGCTCA GGAAAGTGAA CCTGTGGAAG GAGGACTCAG GAAGGTGCCA 
GATCTTCACA AGGATGACCT GGCCCAGCAG AGAATTCAGG GCAGCCTTGC CCCTCACCGC 
GAGCCCCCGA GCTTCATTAC GCTCTCCAAC ATAACAGAAG CTGACTTGGA GACGTGGGAG 
AGACTCAAAG TGTCAGAAAA GGCGAGGGAT GGAGATGTTC AGCACATCTG TGCTTCTGAG 
CCTTCCCCAG AAATTAAAGC AGAAACTGCC ATTCGTGATG ACTTTGCCAA CCGCAAAGCA 
AGGGCCTCTA AGAAAGCTTC CAGCCCCAGG CAAAAGTTTG TGCACTTTGG GCCAGTGACG 
GAGCTAGATC AGCAGAAATG GAAGCGGCTT AGCATTGGCA AGGCTGGGCC TAGAGAGGAT 
GAAGAAGAAG TCATCTGTCA TGGCAGCAAG ATTCAAATGG ACTCTGTGTC TCCTGTCTCA 
GCGGCCACTT CCAGCTTAAA GGGCCACCAA ATATTTAATC GACAAAATGA CTGCAGAACA 
ATGAATTGTG GCCGAGGTGA CTATTGCAGA AGGGCCTCGT GGCTGGCTCC TGTGCCGGAG 
TCTCAGGAAG AGTGGGTCTG CAGTTTGGGC GAGTGCCCGA GGGGGACAGA GGAAGTTACA 
TCCAAACAGC TGCCACAGGA TGGCAAAGAA GAAACAGAAA GCGCTCCAAG AGATTCTGAG 
AGGCTGTCAA AGGCAGAAAG ATCAGAGGAC AGCAGCCAGC CACTGGTGTG TCCTCTGGCC 
TCTGAGTGTG AGGCTTCAGG GACAGAAGAG AAGTTGGAGA AGATGACAGC TCCTGCCTGG 
AGTGGCAGTG GACTGAAAGG CCAGCGAAAG TTGGATGATT CACGAAAAGA TGACATGATG 
GCCAGGAGAA CTGGGATGTC CCTTAGACAC ACTGGATCCA ACCCAAACCA GTTCCTTCCA 
GTTCCATTTG CAAAGCAACA GGATGTGGAA GAATCTTCTA AAGGTCTACC CATGAAAGAT 
CAGAGATACG GTCCGAGAAC TCCTGTGTCT GATGACGCAG AGAGCACCAG CATGTTTGAC 
ATGCGGTGTG AGGAGGAGGC CGCGGTGCAG CCGCACAGCA GGGCCCGCCA GGAGCAGCTG 
CAGCTGATAA ATAACCAGCT GAGGGAAGAG GACGACAAAT GGCAAGATGA CCTGGCTCGT 
TGGAAGAGTC GTAGAAGAAG TGTTTCTCAG GACTTAATCA AGAAAGAGGA AGAAAGGAAA 
AAAATGGAGA AGTTACTGGC TGGAGAAGAT GGGACAAGTG AACGAAGGAA AAGCATCAAA 
ACCTACAGAG AAATTGTTCA AGAAAAAGAG CGGAGAGAGA GAGAGCTGCA TGAAGCATAT 
AAGAACGCTC GGTCCCAGGA GGAGGCAGAG GGGATCCTTC AACAGTACAT TGAGAGGTTC 
ACCATCAGTG AGGCTGTTCT CGAACGCTTG GAGATGCCAA AAATTCTGGA AAGAAGCCAT 
TCAACAGAGC CAAATTTATC CTCCTTCCTG AATGACCCCA ATCCCATGAA ATACCTGCGG 
CAACAGTCAC TGCCTCCACC CAAATTCACT GCCACTGTTG AAACCACCAT TGCTCGTGCC 
AGTGTTCTGG ATACCAGCAT GTCAGCAGGC AGTGGGTCTC CAAGCAAAAC TGTCACTCCC 
AAAGCAGTGC CTATGCTGAC ACCCAAGCCT TACTCCCAGC CCAAAAATTC TCAAGATGTT 
CTGAAGACCT TTAAGGTAGA CGGGAAAGTC AGTGTGAATG GAGAGACGGT TCATAGAGAG 
GAGGAGAAGG AAAGAGAGTG TCCCACGGTG GCACCTGCCC ACTCCTTAAC CAAATCCCAG 
ATGTTTGAAG GTGTGGCCAG AGTGCACGGG TCTCCACTGG AGCTGAAACA AGACAACGGT 
AGCATCGAGA TCAACATAAA GAAGCCAAAC TCTGTTCCCC AAGAGCTCGC AGCAACCACT 
GAGAAAACGG AACCGAATAG TCAAGAGGAC AAGAATGATG GTGGAAAATC AAGAAAAGGG 
AATATAGAAC TTGCCTCATC AGAACCACAG CATTTTACAA CAACTGTGAC TCGATGCAGC 
CCGACCGTGG CCTTTGTGGA ATTTCCCTCC AGCCCCCAGC TGAAGAATGA TGTGTCGGAA 
GAAAAAGACC AGAAGAAACC AGAAAATGAA ATGAGTGGAA AGGTGGAGTT GGTGCTGTCA 
CAAAAGGTGG TAAAGCCAAA ATCTCCAGAA CCCGAAGCAA CGCTGACATT TCCATTTCTG 
GACAAAATGC CTGAAGCCAA CCAACTACAT TTGCCAAATC TCAATTCTCA AGAGTCGCCT 
GGCACTGCCA GTGTTCCGCT TAGAGTTCAG AACTCTTGGC GACGATCCCA GTTTTTCTCC 
CAGTCAGTGG ATTCTCCAAG CAGTGAGAAG TCACCTGTTA TGACACCTCA GTTTAAGTTC 
TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA AATGGCAACA GGAACAGGAA 
CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC TGAAAGAAGA GTGGGAAAAG 
GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG AGGAGGAGCG TAAGATAATT 
GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG CTGACCAGCT GTCTACCTCT 
TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG ACCTGGGAAA CTGTCAAGAT 
GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG ATGACAGTGA CTTATTGCTG 
AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC TAACTGAAGG GGCCTTGGCT 
CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC ATCAGCTGGA TACCGAGGCT 
GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG ATCCATCCCA GAATCAGCAG 
ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA AAACCCTCCC GCTGGATAAA 
AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA AGAAAAGTCC CCGAGAGCAC 
TTCCAGGCTG GGCCTTTCTC TCCCTGTTCT CCCACCCCTC CCGGTCAGTC ACCAAACAGG 
TACAAGAGGT CTATAAGTGG AAAGAAGCTG TGCTCTTCCT GTGGGCTTCC TTTGGGTAAA 
GGAGCTGCAA TGATCATCGA GACCCTCAAT CTCTATTTTC ACATCCAGTG TTTCAGGTGT 
GGAATTTGTA AAGGCCAGCT TGGAGATGCA GTGAGTGGGA CGGATGTTAG GATTCGAAAT 
GGTCTCCTGA ACTGTAATGA TTGCTACATG CGATCCAGAA GTGCCGGGCA GCCTACAACA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_011513651.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511953.1 Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

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CloneID	OHu65260
Clone ID Related Accession (Same CDS sequence)	XM_011513651.2
Accession Version	XM_011513651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and calponin homology domains-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTATGACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATACCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCGCTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511953.1
CDS	1413..5903
Translation	MRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFSFPETIEEEGSEVGSAGEDNPAGQMNPGWKPSDGGCELPDGSGKEHPSSDGAVVAPAPKSEEKDAAEIQKRKRLEQAGIKVMPAAQRFASQKQLSEEKEAIRDIVLRKENSFLTHQHGNDSEAEGEVVCRLPDLEKDDFAARRARMNQTKPMVPLNQLLYGPYPKKGAEKSDGSKQLSKGISKKRSLEYKRNQGHTEEVKLIVTCNMRAQESEPVEGGLRKVPDLHKDDLAQQRIQGSLAPHREPPSFITLSNITEADLETWERLKVSEKARDGDVQHICASEPSPEIKAETAIRDDFANRKARASKKASSPRQKFVHFGPVTELDQQKWKRLSIGKAGPREDEEEVICHGSKIQMDSVSPVSAATSSLKGHQIFNRQNDCRTMNCGRGDYCRRASWLAPVPESQEEWVCSLGECPRGTEEVTSKQLPQDGKEETESAPRDSERLSKAERSEDSSQPLVCPLASECEASGTEEKLEKMTAPAWSGSGLKGQRKLDDSRKDDMMARRTGMSLRHTGSNPNQFLPVPFAKQQDVEESSKGLPMKDQRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQSVDSPSSEKSPVMTPQFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKKSPREHFQAGPFSPCSPTPPGQSPNRYKRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL

Target ORF information:

RefSeq Version	XM_011513651.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and calponin homology domains 1 (LIMCH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513651.2

ORF Insert Sequence:

ATGCGAAAGG ACACTGATGA CATTGAAAGT CCTAAACGCA GTATCCGAGA CAGTGGCTAC 
ATCGACTGCT GGGATTCCGA GCGCAGCGAC TCCCTCTCTC CTCCTCGCCA CGGCAGAGAT 
GATTCCTTCG ACAGCCTGGA TTCCTTTGGC TCTCGCTCTC GGCAGACGCC TTCACCAGAT 
GTAGTCCTCA GGGGAAGCAG CGATGGGAGA GGAAGCGACT CTGAATCCGA CTTGCCTCAT 
CGGAAGCTGC CAGATGTGAA GAAGGATGAC ATGTCTGCAC GGCGGACTTC CCATGGTGAG 
CCGAAATCAG CAGTGCCTTT TAACCAGTAC CTCCCGAACA AAAGCAATCA GACGGCCTAC 
GTCCCCGCGC CTCTGAGAAA GAAGAAAGCA GAGAGAGAGG AATACCGCAA GAGCTGGAGT 
ACCGCCACCT CCCCGCTGGG TGGGGAGAGG CCCTTCAGCT TTCCTGAAAC GATAGAGGAA 
GAGGGGAGTG AAGTGGGGTC TGCTGGTGAA GACAACCCAG CTGGCCAAAT GAATCCTGGT 
TGGAAGCCTT CCGATGGTGG GTGTGAATTA CCTGACGGCA GTGGTAAGGA GCACCCTTCT 
TCAGACGGGG CTGTGGTGGC ACCAGCTCCC AAGTCTGAAG AAAAAGATGC TGCTGAGATC 
CAAAAGCGCA AAAGGCTAGA GCAAGCTGGA ATCAAGGTCA TGCCAGCAGC ACAGCGCTTT 
GCCAGTCAAA AACAATTAAG TGAAGAGAAA GAAGCTATTC GTGATATTGT CCTTCGCAAA 
GAAAACTCTT TCCTGACCCA CCAACATGGC AACGATTCAG AGGCAGAAGG TGAAGTTGTG 
TGTCGACTGC CTGATCTTGA GAAGGATGAC TTTGCTGCAA GGAGAGCAAG GATGAACCAA 
ACCAAGCCAA TGGTGCCATT AAATCAACTC CTCTATGGCC CTTATCCAAA GAAAGGGGCA 
GAGAAATCAG ATGGCAGCAA ACAGCTCTCA AAGGGAATCT CAAAAAAAAG AAGTCTAGAA 
TATAAAAGAA ACCAGGGCCA CACAGAAGAG GTGAAGTTGA TAGTGACCTG TAACATGAGG 
GCTCAGGAAA GTGAACCTGT GGAAGGAGGA CTCAGGAAGG TGCCAGATCT TCACAAGGAT 
GACCTGGCCC AGCAGAGAAT TCAGGGCAGC CTTGCCCCTC ACCGCGAGCC CCCGAGCTTC 
ATTACGCTCT CCAACATAAC AGAAGCTGAC TTGGAGACGT GGGAGAGACT CAAAGTGTCA 
GAAAAGGCGA GGGATGGAGA TGTTCAGCAC ATCTGTGCTT CTGAGCCTTC CCCAGAAATT 
AAAGCAGAAA CTGCCATTCG TGATGACTTT GCCAACCGCA AAGCAAGGGC CTCTAAGAAA 
GCTTCCAGCC CCAGGCAAAA GTTTGTGCAC TTTGGGCCAG TGACGGAGCT AGATCAGCAG 
AAATGGAAGC GGCTTAGCAT TGGCAAGGCT GGGCCTAGAG AGGATGAAGA AGAAGTCATC 
TGTCATGGCA GCAAGATTCA AATGGACTCT GTGTCTCCTG TCTCAGCGGC CACTTCCAGC 
TTAAAGGGCC ACCAAATATT TAATCGACAA AATGACTGCA GAACAATGAA TTGTGGCCGA 
GGTGACTATT GCAGAAGGGC CTCGTGGCTG GCTCCTGTGC CGGAGTCTCA GGAAGAGTGG 
GTCTGCAGTT TGGGCGAGTG CCCGAGGGGG ACAGAGGAAG TTACATCCAA ACAGCTGCCA 
CAGGATGGCA AAGAAGAAAC AGAAAGCGCT CCAAGAGATT CTGAGAGGCT GTCAAAGGCA 
GAAAGATCAG AGGACAGCAG CCAGCCACTG GTGTGTCCTC TGGCCTCTGA GTGTGAGGCT 
TCAGGGACAG AAGAGAAGTT GGAGAAGATG ACAGCTCCTG CCTGGAGTGG CAGTGGACTG 
AAAGGCCAGC GAAAGTTGGA TGATTCACGA AAAGATGACA TGATGGCCAG GAGAACTGGG 
ATGTCCCTTA GACACACTGG ATCCAACCCA AACCAGTTCC TTCCAGTTCC ATTTGCAAAG 
CAACAGGATG TGGAAGAATC TTCTAAAGGT CTACCCATGA AAGATCAGAG ATACGGTCCG 
AGAACTCCTG TGTCTGATGA CGCAGAGAGC ACCAGCATGT TTGACATGCG GTGTGAGGAG 
GAGGCCGCGG TGCAGCCGCA CAGCAGGGCC CGCCAGGAGC AGCTGCAGCT GATAAATAAC 
CAGCTGAGGG AAGAGGACGA CAAATGGCAA GATGACCTGG CTCGTTGGAA GAGTCGTAGA 
AGAAGTGTTT CTCAGGACTT AATCAAGAAA GAGGAAGAAA GGAAAAAAAT GGAGAAGTTA 
CTGGCTGGAG AAGATGGGAC AAGTGAACGA AGGAAAAGCA TCAAAACCTA CAGAGAAATT 
GTTCAAGAAA AAGAGCGGAG AGAGAGAGAG CTGCATGAAG CATATAAGAA CGCTCGGTCC 
CAGGAGGAGG CAGAGGGGAT CCTTCAACAG TACATTGAGA GGTTCACCAT CAGTGAGGCT 
GTTCTCGAAC GCTTGGAGAT GCCAAAAATT CTGGAAAGAA GCCATTCAAC AGAGCCAAAT 
TTATCCTCCT TCCTGAATGA CCCCAATCCC ATGAAATACC TGCGGCAACA GTCACTGCCT 
CCACCCAAAT TCACTGCCAC TGTTGAAACC ACCATTGCTC GTGCCAGTGT TCTGGATACC 
AGCATGTCAG CAGGCAGTGG GTCTCCAAGC AAAACTGTCA CTCCCAAAGC AGTGCCTATG 
CTGACACCCA AGCCTTACTC CCAGCCCAAA AATTCTCAAG ATGTTCTGAA GACCTTTAAG 
GTAGACGGGA AAGTCAGTGT GAATGGAGAG ACGGTTCATA GAGAGGAGGA GAAGGAAAGA 
GAGTGTCCCA CGGTGGCACC TGCCCACTCC TTAACCAAAT CCCAGATGTT TGAAGGTGTG 
GCCAGAGTGC ACGGGTCTCC ACTGGAGCTG AAACAAGACA ACGGTAGCAT CGAGATCAAC 
ATAAAGAAGC CAAACTCTGT TCCCCAAGAG CTCGCAGCAA CCACTGAGAA AACGGAACCG 
AATAGTCAAG AGGACAAGAA TGATGGTGGA AAATCAAGAA AAGGGAATAT AGAACTTGCC 
TCATCAGAAC CACAGCATTT TACAACAACT GTGACTCGAT GCAGCCCGAC CGTGGCCTTT 
GTGGAATTTC CCTCCAGCCC CCAGCTGAAG AATGATGTGT CGGAAGAAAA AGACCAGAAG 
AAACCAGAAA ATGAAATGAG TGGAAAGGTG GAGTTGGTGC TGTCACAAAA GGTGGTAAAG 
CCAAAATCTC CAGAACCCGA AGCAACGCTG ACATTTCCAT TTCTGGACAA AATGCCTGAA 
GCCAACCAAC TACATTTGCC AAATCTCAAT TCTCAAGAGT CGCCTGGCAC TGCCAGTGTT 
CCGCTTAGAG TTCAGAACTC TTGGCGACGA TCCCAGTTTT TCTCCCAGTC AGTGGATTCT 
CCAAGCAGTG AGAAGTCACC TGTTATGACA CCTCAGTTTA AGTTCTGGGC ATGGGACCCA 
GAAGAGGAGC GCAGGCGACA GGAAAAATGG CAACAGGAAC AGGAACGTTT GCTCCAGGAG 
AGATACCAGA AGGAGCAGGA CAAGCTGAAA GAAGAGTGGG AAAAGGCCCA AAAGGAGGTG 
GAAGAGGAAG AACGCAGATA CTATGAGGAG GAGCGTAAGA TAATTGAAGA CACTGTGGTT 
CCATTTACTG TTTCTTCAAG TTCCGCTGAC CAGCTGTCTA CCTCTTCCTC CATGACTGAA 
GGCAGTGGGA CAATGAATAA GATAGACCTG GGAAACTGTC AAGATGAAAA ACAAGACAGA 
AGATGGAAGA AATCATTCCA GGGAGATGAC AGTGACTTAT TGCTGAAGAC TAGGGAAAGT 
GATCGACTGG AGGAGAAGGG CAGCCTAACT GAAGGGGCCT TGGCTCATTC TGGGAACCCT 
GTATCAAAAG GAGTCCATGA AGACCATCAG CTGGATACCG AGGCTGGGGC CCCACACTGT 
GGAACAAACC CACAGCTTGC TCAGGATCCA TCCCAGAATC AGCAGACATC AAATCCAACG 
CACAGTTCAG AAGATGTGAA GCCAAAAACC CTCCCGCTGG ATAAAAGCAT TAACCATCAG 
ATCGAGTCTC CCAGTGAAAG GCGGAAGAAA AGTCCCCGAG AGCACTTCCA GGCTGGGCCT 
TTCTCTCCCT GTTCTCCCAC CCCTCCCGGT CAGTCACCAA ACAGGTACAA GAGGTCTATA 
AGTGGAAAGA AGCTGTGCTC TTCCTGTGGG CTTCCTTTGG GTAAAGGAGC TGCAATGATC 
ATCGAGACCC TCAATCTCTA TTTTCACATC CAGTGTTTCA GGTGTGGAAT TTGTAAAGGC 
CAGCTTGGAG ATGCAGTGAG TGGGACGGAT GTTAGGATTC GAAATGGTCT CCTGAACTGT 
AATGATTGCT ACATGCGATC CAGAAGTGCC GGGCAGCCTA CAACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration.
Molecular biology of the cell28(8)1054-1065(2017 Apr)
Lin YH,Zhen YY,Chien KY,Lee IC,Lin WC,Chen MY,Pai LM

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オンライン注文

LIMCH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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