GBF1 cDNA ORF clone, Homo sapiens(human)

The following GBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu91777 XM_017016864.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu108495 NM_001199379.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu108497 NM_001199378.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu108697 NM_004193.3
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu108697 NM_001377141.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu109403 NM_001377138.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu109463 NM_001377139.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu109500 NM_001377140.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu44657 NM_001377137.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu91774 XM_017016861.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu91775 XM_017016862.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu91776 XM_017016863.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu06003 NM_001199378.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu11757 NM_001199379.1
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu55708 NM_004193.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44653 XM_006718047.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44654 XM_006718048.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44655 XM_006718049.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44656 XM_006718050.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44657 XM_005270261.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu62151 XM_011540312.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu62152 XM_011540313.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu62153 XM_011540314.2
Latest version!
Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu44654 XM_006718048.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44656 XM_006718050.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu91775 XM_017016862.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu62151 XM_011540312.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu62152 XM_011540313.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu44657 XM_005270261.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu91777 XM_017016864.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu91776 XM_017016863.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu44653 XM_006718047.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu44655 XM_006718049.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu91774 XM_017016861.1 Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu91777
    Clone ID Related Accession (Same CDS sequence) XM_017016864.1 , XM_017016864.2
    Accession Version XM_017016864.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
    GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC
    CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA
    GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG
    GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG
    GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC
    CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC
    GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT
    ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA
    GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG
    GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA
    GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA
    GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT
    AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA
    AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA
    CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA
    GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG
    GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT
    TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC
    CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG
    CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG
    ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG
    TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA
    CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC
    CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG
    ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG
    GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT
    CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG
    TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT
    GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG
    TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG
    AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG
    GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT
    TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT
    ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA
    GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC
    CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA
    CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG
    GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG
    ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG
    TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG
    GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG
    AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA
    GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC
    CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA
    GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT
    GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC
    GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC
    AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG
    CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC
    CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT
    CCCATACCCA CCTCTGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872353.1
    CDS2090..5176
    Translation

    Target ORF information:

    RefSeq Version XM_017016864.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
    GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC
    CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA
    GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG
    GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG
    GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC
    CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC
    GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT
    ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA
    GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG
    GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA
    GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA
    GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT
    AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA
    AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA
    CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA
    GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG
    GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT
    TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC
    CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG
    CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG
    ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG
    TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA
    CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC
    CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG
    ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG
    GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT
    CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG
    TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT
    GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG
    TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG
    AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG
    GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT
    TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT
    ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA
    GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC
    CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA
    CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG
    GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG
    ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG
    TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG
    GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG
    AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA
    GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC
    CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA
    GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT
    GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC
    GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC
    AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG
    CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC
    CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT
    CCCATACCCA CCTCTGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91777
    Clone ID Related Accession (Same CDS sequence) XM_017016864.1 , XM_017016864.2
    Accession Version XM_017016864.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
    GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC
    CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA
    GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG
    GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG
    GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC
    CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC
    GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT
    ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA
    GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG
    GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA
    GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA
    GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT
    AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA
    AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA
    CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA
    GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG
    GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT
    TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC
    CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG
    CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG
    ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG
    TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA
    CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC
    CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG
    ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG
    GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT
    CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG
    TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT
    GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG
    TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG
    AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG
    GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT
    TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT
    ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA
    GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC
    CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA
    CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG
    GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG
    ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG
    TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG
    GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG
    AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA
    GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC
    CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA
    GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT
    GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC
    GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC
    AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG
    CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC
    CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT
    CCCATACCCA CCTCTGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872353.1
    CDS1990..5076
    Translation

    Target ORF information:

    RefSeq Version XM_017016864.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016864.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTTAATA CTGACCAGCA CAACCACAAT GTTCGTAAAC AGAATGCACC CATGACCCTG 
    GAGGAGTTTC GCAAAAATCT GAAAGGTGTG AATGGAGGCA AGGACTTTGA GCAAGACATC
    CTGGAGGACA TGTACCATGC CATCAAGAAT GAGGAAATTG TAATGCCTGA GGAGCAGACA
    GGCTTGGTTC GGGAGAACTA TGTGTGGAAT GTGCTGCTTC ATCGAGGTGC CACCCCTGAG
    GGCATATTCC TGCGTGTGCC TACTGCCAGC TATGATCTTG ACCTCTTCAC CATGACCTGG
    GGCCCCACTA TTGCTGCTCT CTCTTATGTC TTTGACAAAA GCCTTGAGGA GACAATCATC
    CAGAAAGCCA TCTCAGGCTT CAGGAAGTGC GCCATGATCT CCGCCCACTA TGGCCTCAGC
    GATGTGTTTG ACAATCTCAT CATCTCTCTA TGCAAATTCA CAGCTCTCAG CAGTGAGTCT
    ATTGAGAACC TGCCCAGTGT ATTTGGAAGC AACCCTAAAG CCCATATTGC AGCCAAGACA
    GTATTCCATT TGGCCCATCG TCATGGTGAC ATCCTGCGGG AGGGCTGGAA GAATATCATG
    GAGGCCATGC TGCAGCTCTT CCGAGCCCAA CTACTGCCCA AGGCTATGAT AGAGGTAGAA
    GATTTCGTGG ATCCCAATGG CAAGATCTCT CTACAGCGGG AAGAGACACC ATCAAACCGA
    GGAGAGTCAA CAGTGCTGAG CTTTGTGAGC TGGCTAACAC TGAGTGGTCC TGAGCAGTCT
    AGTGTTCGGG GCCCATCCAC TGAAAACCAA GAGGCCAAGA GAGTGGCCTT AGAGTGTATA
    AAGCAATGTG ACCCAGAAAA AATGATCACA GAAAGCAAGT TCCTCCAGCT GGAGTCACTA
    CAGGAGCTCA TGAAGGCTCT GGTCTCAGTG ACACCAGATG AAGAGACATA TGATGAGGAA
    GATGCTGCTT TCTGCCTAGA GATGCTGCTA AGGATTGTGT TGGAGAACAG GGATCGTGTG
    GGCTGTGTGT GGCAGACTGT TCGAGACCAT CTATACCACC TCTGTGTTCA GGCACAAGAT
    TTCTGCTTCC TTGTGGAGCG GGCAGTGGTG GGGTTGCTAC GCCTGGCCAT TCGGCTTCTC
    CGGAGAGAAG AGATCAGTGC TCAGGTGCTG CTCTCCCTGC GCATTTTGCT ACTGATGAAG
    CCCAGTGTGC TATCCCGAGT CAGCCACCAG GTTGCGTATG GGCTCCATGA ACTCCTGAAG
    ACCAATGCAG CCAACATCCA CTCAGGTGAT GACTGGGCCA CACTCTTCAC ACTGCTGGAG
    TGCATCGGCT CAGGTGTGAA GCCTCCAGCT GCTCTGCAGG CCACAGCCAG GGCAGATGCA
    CCTGATGCCG GGGCCCAGTC AGATAGTGAG CTCCCATCCT ACCATCAGAA TGACGTGAGC
    CTGGATCGAG GGTACACTTC CGACTCAGAG GTCTACACTG ACCATGGCAG GCCGGGCAAG
    ATACACCGAT CAGCCACAGA TGCCGATGTG GTCAACAGTG GTTGGTTAGT GGTTGGGAAG
    GATGACGTTG ATAACTCCAA GCCAGGGCCC AGCCGCCCAG GCCCTTCACC CCTGATCAAT
    CAATACAGCC TAACAGTGGG ACTGGATTTG GGGCCACACG ACACTAAGTC TCTGCTTAAG
    TGTGTGGAAT CGCTGTCCTT CATTGTGCGT GATGCTGCCC ACATCACACC TGACAACTTT
    GAGCTCTGCG TCAAGACTCT CCGGATCTTT GTGGAGGCCA GTCTGAATGG CGGATGCAAG
    TCCCAGGAGA AACGTGGCAA GAGTCACAAA TATGACAGCA AAGGGAACCG CTTCAAGAAG
    AAATCCAAAG AGGGATCAAT GCTTCGCCGG CCTCGAACCT CCAGCCAACA TGCCTCTCGG
    GGCGGGCAGA GTGATGATGA TGAGGACGAA GGCGTGCCTG CCAGCTACCA TACGGTGTCT
    TTACAGGTCA GTCAGGACTT GCTAGACCTG ATGCACACCC TGCACACGCG GGCAGCCTCT
    ATCTACAGCT CATGGGCGGA GGAGCAACGC CACCTGGAGA CAGGTGGCCA GAAGATTGAA
    GCTGATTCTC GCACCCTCTG GGCCCACTGC TGGTGCCCTT TACTGCAGGG TATTGCCTGC
    CTGTGCTGCG ATGCCCGGCG CCAGGTACGG ATGCAGGCAC TGACCTATCT GCAGCGAGCA
    CTACTTGTAC ATGATCTGCA AAAGCTAGAT GCCCTGGAAT GGGAGTCCTG TTTTAACAAG
    GTGCTGTTTC CTCTACTTAC CAAGCTCTTG GAGAACATCA GCCCTGCAGA TGTGGGTGGG
    ATGGAGGAGA CCCGGATGAG GGCTTCCACA TTGCTCTCTA AGGTCTTCCT GCAGCACCTG
    TCTCCACTGC TGTCACTCTC TACCTTTGCG GCCCTCTGGC TCACCATCTT GGACTTCATG
    GACAAGTACA TGCACGCAGG CTCCAGCGAC TTACTGTCAG AGGCGATCCC TGAGTCTCTG
    AAGAACATGC TTCTGGTGAT GGACACAGCG GAGATTTTCC ACAGTGCAGA TGCACGGGGA
    GGCGGCCCCT CGGCCCTCTG GGAGATCACC TGGGAACGCA TTGACTGTTT TCTCCCTCAC
    CTACGAGATG AACTCTTCAA GCAGACCGTC ATCCAGGACC CCATGCCCAT GGAGCCTCAA
    GGCCAAAAGC CTCTCGCCTC AGCCCACCTG ACTTCCGCTG CTGGCGACAC TAGGACACCT
    GGCCATCCAC CGCCCCCAGA GATTCCATCT GAGCTGGGGG CCTGTGACTT TGAGAAGCCC
    GAGAGCCCCC GAGCCGCCAG CAGCAGCTCC CCAGGATCAC CAGTGGCCTC AAGCCCCAGC
    AGGCTGAGCC CCACCCCCGA CGGGCCTCCA CCCTTGGCTC AGCCCCCACT GATCCTGCAG
    CCCTTGGCCT CCCCACTGCA GGTGGGCGTG CCACCTATGA CTCTGCCCAT CATCCTCAAC
    CCTGCGCTCA TCGAGGCCAC CTCACCAGTG CCCCTCCTGG CCACACCCCG CCCCACAGAT
    CCCATACCCA CCTCTGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108495
    Clone ID Related Accession (Same CDS sequence) NM_001199379.2
    Accession Version NM_001199379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_001199379.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31632.1, SRR1803614.50084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC
    CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG
    ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT
    TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA
    CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA
    TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC
    AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT
    AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG
    CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA
    GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC
    CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC
    ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC
    CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT
    GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG
    GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA
    CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT
    CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG
    ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG
    GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186308.1
    CDS261..5828
    Translation

    Target ORF information:

    RefSeq Version NM_001199379.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC
    CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG
    ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT
    TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA
    CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA
    TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC
    AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT
    AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG
    CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA
    GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC
    CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC
    ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC
    CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT
    GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG
    GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA
    CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT
    CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG
    ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG
    GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108497
    Clone ID Related Accession (Same CDS sequence) NM_001199378.2
    Accession Version NM_001199378.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_001199378.1. Transcript Variant: This variant (2) uses multiple alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK025330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186307.1
    CDS261..5831
    Translation

    Target ORF information:

    RefSeq Version NM_001199378.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108697
    Clone ID Related Accession (Same CDS sequence) NM_004193.3 , NM_001377141.1
    Accession Version NM_004193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Jun 2, 2019 this sequence version replaced NM_004193.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.201996.1, AF068755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004184.1
    CDS261..5840
    Translation

    Target ORF information:

    RefSeq Version NM_004193.3
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108697
    Clone ID Related Accession (Same CDS sequence) NM_004193.3 , NM_001377141.1
    Accession Version NM_001377141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Transcript Variant: This variant (8), as well as variant 1, encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.183024.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001364070.1
    CDS360..5939
    Translation

    Target ORF information:

    RefSeq Version NM_001377141.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001377141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATTGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109403
    Clone ID Related Accession (Same CDS sequence) NM_001377138.1
    Accession Version NM_001377138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGTTGCTAG ACCTGATGCA CACCCTGCAC ACGCGGGCAG CCTCTATCTA CAGCTCATGG
    GCGGAGGAGC AACGCCACCT GGAGACAGGT GGCCAGAAGA TTGAAGCTGA TTCTCGCACC
    CTCTGGGCCC ACTGCTGGTG CCCTTTACTG CAGGGTATTG CCTGCCTGTG CTGCGATGCC
    CGGCGCCAGG TACGGATGCA GGCACTGACC TATCTGCAGC GAGCACTACT TGTACATGAT
    CTGCAAAAGC TAGATGCCCT GGAATGGGAG TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA
    CTTACCAAGC TCTTGGAGAA CATCAGCCCT GCAGATGTGG GTGGGATGGA GGAGACCCGG
    ATGAGGGCTT CCACATTGCT CTCTAAGGTC TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA
    CTCTCTACCT TTGCGGCCCT CTGGCTCACC ATCTTGGACT TCATGGACAA GTACATGCAC
    GCAGGCTCCA GCGACTTACT GTCAGAGGCG ATCCCTGAGT CTCTGAAGAA CATGCTTCTG
    GTGATGGACA CAGCGGAGAT TTTCCACAGT GCAGATGCAC GGGGAGGCGG CCCCTCGGCC
    CTCTGGGAGA TCACCTGGGA ACGCATTGAC TGTTTTCTCC CTCACCTACG AGATGAACTC
    TTCAAGCAGA CCGTCATCCA GGACCCCATG CCCATGGAGC CTCAAGGCCA AAAGCCTCTC
    GCCTCAGCCC ACCTGACTTC CGCTGCTGGC GACACTAGGA CACCTGGCCA TCCACCGCCC
    CCAGAGATTC CATCTGAGCT GGGGGCCTGT GACTTTGAGA AGCCCGAGAG CCCCCGAGCC
    GCCAGCAGCA GCTCCCCAGG ATCACCAGTG GCCTCAAGCC CCAGCAGGCT GAGCCCCACC
    CCCGACGGGC CTCCACCCTT GGCTCAGCCC CCACTGATCC TGCAGCCCTT GGCCTCCCCA
    CTGCAGGTGG GCGTGCCACC TATGACTCTG CCCATCATCC TCAACCCTGC GCTCATCGAG
    GCCACCTCAC CAGTGCCCCT CCTGGCCACA CCCCGCCCCA CAGATCCCAT ACCCACCTCT
    GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001364067.1
    CDS261..5792
    Translation

    Target ORF information:

    RefSeq Version NM_001377138.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001377138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGTTGCTAG ACCTGATGCA CACCCTGCAC ACGCGGGCAG CCTCTATCTA CAGCTCATGG
    GCGGAGGAGC AACGCCACCT GGAGACAGGT GGCCAGAAGA TTGAAGCTGA TTCTCGCACC
    CTCTGGGCCC ACTGCTGGTG CCCTTTACTG CAGGGTATTG CCTGCCTGTG CTGCGATGCC
    CGGCGCCAGG TACGGATGCA GGCACTGACC TATCTGCAGC GAGCACTACT TGTACATGAT
    CTGCAAAAGC TAGATGCCCT GGAATGGGAG TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA
    CTTACCAAGC TCTTGGAGAA CATCAGCCCT GCAGATGTGG GTGGGATGGA GGAGACCCGG
    ATGAGGGCTT CCACATTGCT CTCTAAGGTC TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA
    CTCTCTACCT TTGCGGCCCT CTGGCTCACC ATCTTGGACT TCATGGACAA GTACATGCAC
    GCAGGCTCCA GCGACTTACT GTCAGAGGCG ATCCCTGAGT CTCTGAAGAA CATGCTTCTG
    GTGATGGACA CAGCGGAGAT TTTCCACAGT GCAGATGCAC GGGGAGGCGG CCCCTCGGCC
    CTCTGGGAGA TCACCTGGGA ACGCATTGAC TGTTTTCTCC CTCACCTACG AGATGAACTC
    TTCAAGCAGA CCGTCATCCA GGACCCCATG CCCATGGAGC CTCAAGGCCA AAAGCCTCTC
    GCCTCAGCCC ACCTGACTTC CGCTGCTGGC GACACTAGGA CACCTGGCCA TCCACCGCCC
    CCAGAGATTC CATCTGAGCT GGGGGCCTGT GACTTTGAGA AGCCCGAGAG CCCCCGAGCC
    GCCAGCAGCA GCTCCCCAGG ATCACCAGTG GCCTCAAGCC CCAGCAGGCT GAGCCCCACC
    CCCGACGGGC CTCCACCCTT GGCTCAGCCC CCACTGATCC TGCAGCCCTT GGCCTCCCCA
    CTGCAGGTGG GCGTGCCACC TATGACTCTG CCCATCATCC TCAACCCTGC GCTCATCGAG
    GCCACCTCAC CAGTGCCCCT CCTGGCCACA CCCCGCCCCA CAGATCCCAT ACCCACCTCT
    GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109463
    Clone ID Related Accession (Same CDS sequence) NM_001377139.1
    Accession Version NM_001377139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT
    GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC
    AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC
    ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001364068.1
    CDS261..5546
    Translation

    Target ORF information:

    RefSeq Version NM_001377139.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001377139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT
    GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC
    AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC
    ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109500
    Clone ID Related Accession (Same CDS sequence) NM_001377140.1
    Accession Version NM_001377140.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.198144.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT
    GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC
    AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC
    ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001364069.1
    CDS261..5534
    Translation

    Target ORF information:

    RefSeq Version NM_001377140.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001377140.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGA ATGCCTTCCC TGTGTCTGGT CAACTCTATA CAACACACCT ACTATCTCTT
    GATGCCCTAT TGACAGTGAT TGACAGCACC GAGGCCCACT GCCAGGCTAA AGTCCTCAAC
    AGCCTCACCC AGCAAGAGAA GAAGGAGACA GCCAGACCAA GCTGTGAGAT AGTAGATGGC
    ACCCGAGAAG CTAGCAATAC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44657
    Clone ID Related Accession (Same CDS sequence) XM_005270261.1 , NM_001377137.1 , XM_005270261.2
    Accession Version NM_001377137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160011.35, AL356420.14 and AL121928.13. On Dec 17, 2019 this sequence version replaced XM_005270261.2. Transcript Variant: This variant (4) encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001364066.1
    CDS261..5843
    Translation

    Target ORF information:

    RefSeq Version NM_001377137.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001377137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91774
    Clone ID Related Accession (Same CDS sequence) XM_017016861.1 , XM_017016861.2
    Accession Version XM_017016861.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA
    ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG
    CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC
    ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC
    TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA
    TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA
    GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC
    CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT
    GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC
    ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA
    GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC
    ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG
    ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG
    GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT
    GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA
    ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT
    TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT
    GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA
    CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC
    CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT
    AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG
    ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT
    GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA
    GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA
    AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT
    GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA
    AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA
    GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT
    GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG
    GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA
    GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC
    TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT
    ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT
    CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC
    ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT
    CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC
    CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT
    ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC
    ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT
    GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC
    CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT
    TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG
    CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG
    GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA
    ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG
    GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT
    GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC
    ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT
    TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG
    TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC
    CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA
    GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG
    CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA
    GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC
    TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC
    GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA
    GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA
    TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT
    GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC
    CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA
    TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC
    GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG
    AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA
    GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG
    AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC
    AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC
    TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT
    CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC
    GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA
    CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT
    CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG
    ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG
    CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC
    ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG
    CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC
    TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT
    GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG
    CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA
    CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC
    CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC
    CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC
    TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC
    ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC
    ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872350.1
    CDS295..5952
    Translation

    Target ORF information:

    RefSeq Version XM_017016861.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA
    ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG
    CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC
    ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC
    TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA
    TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA
    GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC
    CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT
    GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC
    ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA
    GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC
    ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG
    ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG
    GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT
    GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA
    ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT
    TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT
    GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA
    CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC
    CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT
    AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG
    ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT
    GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA
    GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA
    AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT
    GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA
    AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA
    GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT
    GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG
    GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA
    GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC
    TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT
    ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT
    CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC
    ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT
    CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC
    CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT
    ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC
    ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT
    GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC
    CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT
    TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG
    CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG
    GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA
    ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG
    GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT
    GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC
    ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT
    TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG
    TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC
    CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA
    GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG
    CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA
    GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC
    TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC
    GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA
    GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA
    TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT
    GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC
    CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA
    TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC
    GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG
    AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA
    GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG
    AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC
    AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC
    TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT
    CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC
    GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA
    CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT
    CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG
    ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG
    CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC
    ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG
    CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC
    TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT
    GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG
    CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA
    CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC
    CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC
    CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC
    TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC
    ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC
    ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91774
    Clone ID Related Accession (Same CDS sequence) XM_017016861.1 , XM_017016861.2
    Accession Version XM_017016861.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA
    ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG
    CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC
    ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC
    TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA
    TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA
    GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC
    CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT
    GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC
    ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA
    GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC
    ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG
    ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG
    GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT
    GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA
    ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT
    TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT
    GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA
    CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC
    CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT
    AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG
    ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT
    GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA
    GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA
    AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT
    GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA
    AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA
    GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT
    GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG
    GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA
    GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC
    TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT
    ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT
    CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC
    ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT
    CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC
    CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT
    ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC
    ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT
    GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC
    CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT
    TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG
    CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG
    GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA
    ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG
    GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT
    GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC
    ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT
    TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG
    TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC
    CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA
    GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG
    CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA
    GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC
    TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC
    GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA
    GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA
    TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT
    GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC
    CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA
    TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC
    GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG
    AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA
    GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG
    AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC
    AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC
    TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT
    CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC
    GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA
    CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT
    CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG
    ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG
    CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC
    ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG
    CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC
    TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT
    GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG
    CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA
    CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC
    CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC
    CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC
    TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC
    ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC
    ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872350.1
    CDS262..5919
    Translation

    Target ORF information:

    RefSeq Version XM_017016861.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGCTGAAA
    ATGAGAGCCG GAGGCATGAG TGATTCATCC AAATGGAAGA AACAGAAGAG ATCCCCTCGG
    CCCCCACGCC ATATGACCAA AGTCACACCA GGTTCAGAGC TGCCCACTCC CAATGGAACC
    ACCTTATCAT CTAACCTCAC TGGTGGCATG CCCTTCATTG ATGTGCCCAC TCCCATCTCC
    TCTGCAAGTT CAGAAGCTGC CTCAGCAGTG GTCAGTCCCT CTACAGACAG TGGCCTGGAA
    TTCTCCTCCC AAACCACTTC CAAGGAAGAC CTTACTGATC TAGAGCAACC TGGCTCTCCA
    GGGTACAGCA CAGCTACAGA GCCTGGAAGC AGTGAGCTAG GTGTTCCCGA GCAGCCTGAC
    CTCCAGCAGG AAGGGACCCA TGTGGAAAAG TCCCAGTCAG CATCTGTGGA GTCCATCCCT
    GAAGTGTTAG AGGAGTGCAC GTCCCCTGCC GACCACTCTG ACTCTGCCTC TGTCCATGAC
    ATGGATTACG TCAATCCCCG GGGCGTGCGC TTTACACAGT CCTCCCAGAA AGAAGGCACA
    GCTTTGGTCC CCTATGGTCT TCCCTGCATC CGCGAGCTCT TCCGCTTCCT CATCTCCCTC
    ACCAATCCAC ACGACCGCCA TAACTCAGAG GTTATGATTC ACATGGGACT GCATTTGCTG
    ACAGTGGCCC TTGAGTCAGC CCCTGTAGCC CAGTGCCAGA CCCTCTTGGG CCTCATCAAG
    GATGAGATGT GCCGTCACTT ATTCCAGCTA CTCAGCATAG AGCGACTAAA CCTTTATGCT
    GCTTCCCTGC GAGTATGCTT CCTACTGTTT GAGAGCATGC GAGAGCACCT CAAGTTCCAA
    ATGGAGATGT ACATCAAAAA GCTTATGGAG ATCATCACTG TGGAGAACCC CAAGATGCCT
    TATGAGATGA AGGAGATGGC ACTGGAGGCC ATTGTGCAGC TCTGGCGCAT CCCCAGCTTT
    GTCACAGAGC TCTACATCAA CTATGATTGT GACTACTACT GTTCCAACCT CTTTGAGGAA
    CTCACAAAGC TGCTGTCCAA GAATGCCTTC CCTGTGTCTG GTCAACTCTA TACAACACAC
    CTACTATCTC TTGATGCCCT ATTGACAGTG ATTGACAGCA CCGAGGCCCA CTGCCAGGCT
    AAAGTCCTCA ACAGCCTCAC CCAGCAAGAG AAGAAGGAGA CAGCCAGACC AAGCTGTGAG
    ATAGTAGATG GCACCCGAGA AGCTAGCAAT ACTGAGAGAA CTGCCAGCGA TGGGAAAGCT
    GTAGGCATGG CCTCAGACAT CCCAGGCCTG CATCTGCCAG GTGGAGGGCG GCTGCCACCA
    GAACATGGGA AATCAGGATG CAGTGATCTG GAGGAAGCTG TTGACTCTGG GGCTGACAAA
    AAGTTTGCCC GGAAGCCACC CCGATTTTCC TGTCTCCTGC CAGATCCACG GGAACTAATT
    GAAATTAAAA ACAAAAAGAA GCTGCTAATC ACTGGCACAG AGCAGTTCAA TCAGAAACCA
    AAGAAGGGGA TTCAGTTTCT GCAAGAGAAA GGCCTCCTCA CCATCCCAAT GGACAACACA
    GAGGTTGCTC AGTGGCTCCG AGAGAACCCT CGGCTGGACA AGAAGATGAT TGGAGAGTTT
    GTGAGTGACC GCAAAAACAT TGACCTGTTG GAGAGCTTTG TGAGCACCTT CAGTTTTCAG
    GGTCTGCGAC TGGACGAAGC CCTCCGCCTC TACCTGGAAG CCTTCCGTTT GCCTGGGGAA
    GCACCAGTCA TCCAGAGGTT GCTGGAGGCA TTCACAGAGC GTTGGATGAA TTGTAATGGC
    TCCCCATTTG CCAATAGCGA TGCCTGCTTT TCCCTGGCCT ATGCTGTCAT CATGCTTAAT
    ACTGACCAGC ACAACCACAA TGTTCGTAAA CAGAATGCAC CCATGACCCT GGAGGAGTTT
    CGCAAAAATC TGAAAGGTGT GAATGGAGGC AAGGACTTTG AGCAAGACAT CCTGGAGGAC
    ATGTACCATG CCATCAAGAA TGAGGAAATT GTAATGCCTG AGGAGCAGAC AGGCTTGGTT
    CGGGAGAACT ATGTGTGGAA TGTGCTGCTT CATCGAGGTG CCACCCCTGA GGGCATATTC
    CTGCGTGTGC CTACTGCCAG CTATGATCTT GACCTCTTCA CCATGACCTG GGGCCCCACT
    ATTGCTGCTC TCTCTTATGT CTTTGACAAA AGCCTTGAGG AGACAATCAT CCAGAAAGCC
    ATCTCAGGCT TCAGGAAGTG CGCCATGATC TCCGCCCACT ATGGCCTCAG CGATGTGTTT
    GACAATCTCA TCATCTCTCT ATGCAAATTC ACAGCTCTCA GCAGTGAGTC TATTGAGAAC
    CTGCCCAGTG TATTTGGAAG CAACCCTAAA GCCCATATTG CAGCCAAGAC AGTATTCCAT
    TTGGCCCATC GTCATGGTGA CATCCTGCGG GAGGGCTGGA AGAATATCAT GGAGGCCATG
    CTGCAGCTCT TCCGAGCCCA ACTACTGCCC AAGGCTATGA TAGAGGTAGA AGATTTCGTG
    GATCCCAATG GCAAGATCTC TCTACAGCGG GAAGAGACAC CATCAAACCG AGGAGAGTCA
    ACAGTGCTGA GCTTTGTGAG CTGGCTAACA CTGAGTGGTC CTGAGCAGTC TAGTGTTCGG
    GGCCCATCCA CTGAAAACCA AGAGGCCAAG AGAGTGGCCT TAGAGTGTAT AAAGCAATGT
    GACCCAGAAA AAATGATCAC AGAAAGCAAG TTCCTCCAGC TGGAGTCACT ACAGGAGCTC
    ATGAAGGCTC TGGTCTCAGT GACACCAGAT GAAGAGACAT ATGATGAGGA AGATGCTGCT
    TTCTGCCTAG AGATGCTGCT AAGGATTGTG TTGGAGAACA GGGATCGTGT GGGCTGTGTG
    TGGCAGACTG TTCGAGACCA TCTATACCAC CTCTGTGTTC AGGCACAAGA TTTCTGCTTC
    CTTGTGGAGC GGGCAGTGGT GGGGTTGCTA CGCCTGGCCA TTCGGCTTCT CCGGAGAGAA
    GAGATCAGTG CTCAGGTGCT GCTCTCCCTG CGCATTTTGC TACTGATGAA GCCCAGTGTG
    CTATCCCGAG TCAGCCACCA GGTTGCGTAT GGGCTCCATG AACTCCTGAA GACCAATGCA
    GCCAACATCC ACTCAGGTGA TGACTGGGCC ACACTCTTCA CACTGCTGGA GTGCATCGGC
    TCAGGTGTGA AGCCTCCAGC TGCTCTGCAG GCCACAGCCA GGGCAGATGC ACCTGATGCC
    GGGGCCCAGT CAGATAGTGA GCTCCCATCC TACCATCAGA ATGACGTGAG CCTGGATCGA
    GGGTACACTT CCGACTCAGA GGTCTACACT GACCATGGCA GGCCGGGCAA GATACACCGA
    TCAGCCACAG ATGCCGATGT GGTCAACAGT GGTTGGTTAG TGGTTGGGAA GGATGACGTT
    GATAACTCCA AGCCAGGGCC CAGCCGCCCA GGCCCTTCAC CCCTGATCAA TCAATACAGC
    CTAACAGTGG GACTGGATTT GGGGCCACAC GACACTAAGT CTCTGCTTAA GTGTGTGGAA
    TCGCTGTCCT TCATTGTGCG TGATGCTGCC CACATCACAC CTGACAACTT TGAGCTCTGC
    GTCAAGACTC TCCGGATCTT TGTGGAGGCC AGTCTGAATG GCGGATGCAA GTCCCAGGAG
    AAACGTGGCA AGAGTCACAA ATATGACAGC AAAGGGAACC GCTTCAAGAA GAAATCCAAA
    GAGGGATCAA TGCTTCGCCG GCCTCGAACC TCCAGCCAAC ATGCCTCTCG GGGCGGGCAG
    AGTGATGATG ATGAGGACGA AGGCGTGCCT GCCAGCTACC ATACGGTGTC TTTACAGGTC
    AGTCAGGACT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC
    TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT
    CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC
    GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA
    CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT
    CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG
    ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG
    CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC
    ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG
    CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC
    TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT
    GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG
    CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA
    CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC
    CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC
    CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC
    TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC
    ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC
    ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91775
    Clone ID Related Accession (Same CDS sequence) XM_017016862.1 , XM_017016862.2
    Accession Version XM_017016862.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872351.1
    CDS295..5928
    Translation

    Target ORF information:

    RefSeq Version XM_017016862.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91775
    Clone ID Related Accession (Same CDS sequence) XM_017016862.1 , XM_017016862.2
    Accession Version XM_017016862.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872351.1
    CDS262..5895
    Translation

    Target ORF information:

    RefSeq Version XM_017016862.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016862.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGCT AGACCTGATG
    CACACCCTGC ACACGCGGGC AGCCTCTATC TACAGCTCAT GGGCGGAGGA GCAACGCCAC
    CTGGAGACAG GTGGCCAGAA GATTGAAGCT GATTCTCGCA CCCTCTGGGC CCACTGCTGG
    TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG TGCTGCGATG CCCGGCGCCA GGTACGGATG
    CAGGCACTGA CCTATCTGCA GCGAGCACTA CTTGTACATG ATCTGCAAAA GCTAGATGCC
    CTGGAATGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTTACCAA GCTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACCC GGATGAGGGC TTCCACATTG
    CTCTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTCA CCATCTTGGA CTTCATGGAC AAGTACATGC ACGCAGGCTC CAGCGACTTA
    CTGTCAGAGG CGATCCCTGA GTCTCTGAAG AACATGCTTC TGGTGATGGA CACAGCGGAG
    ATTTTCCACA GTGCAGATGC ACGGGGAGGC GGCCCCTCGG CCCTCTGGGA GATCACCTGG
    GAACGCATTG ACTGTTTTCT CCCTCACCTA CGAGATGAAC TCTTCAAGCA GACCGTCATC
    CAGGACCCCA TGCCCATGGA GCCTCAAGGC CAAAAGCCTC TCGCCTCAGC CCACCTGACT
    TCCGCTGCTG GCGACACTAG GACACCTGGC CATCCACCGC CCCCAGAGAT TCCATCTGAG
    CTGGGGGCCT GTGACTTTGA GAAGCCCGAG AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA
    GGATCACCAG TGGCCTCAAG CCCCAGCAGG CTGAGCCCCA CCCCCGACGG GCCTCCACCC
    TTGGCTCAGC CCCCACTGAT CCTGCAGCCC TTGGCCTCCC CACTGCAGGT GGGCGTGCCA
    CCTATGACTC TGCCCATCAT CCTCAACCCT GCGCTCATCG AGGCCACCTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGATCCC ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91776
    Clone ID Related Accession (Same CDS sequence) XM_017016863.2 , XM_017016863.1
    Accession Version XM_017016863.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC
    TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT
    GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG
    TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA
    CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG
    CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG
    GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT
    CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC
    AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG
    AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC
    GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA
    CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC
    CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC
    CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG
    AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG
    CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC
    TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT
    GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC
    ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872352.1
    CDS262..5805
    Translation

    Target ORF information:

    RefSeq Version XM_017016863.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016863.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC
    TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT
    GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG
    TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA
    CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG
    CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG
    GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT
    CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC
    AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG
    AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC
    GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA
    CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC
    CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC
    CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG
    AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG
    CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC
    TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT
    GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC
    ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu91776
    Clone ID Related Accession (Same CDS sequence) XM_017016863.2 , XM_017016863.1
    Accession Version XM_017016863.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC
    TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT
    GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG
    TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA
    CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG
    CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG
    GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT
    CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC
    AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG
    AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC
    GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA
    CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC
    CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC
    CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG
    AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG
    CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC
    TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT
    GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC
    ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872352.1
    CDS295..5838
    Translation

    Target ORF information:

    RefSeq Version XM_017016863.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTCTG AGAGAACTGC CAGCGATGGG
    AAAGCTGTAG GCATGGCCTC AGACATCCCA GGCCTGCATC TGCCAGGTGG AGGGCGGCTG
    CCACCAGAAC ATGGGAAATC AGGATGCAGT GATCTGGAGG AAGCTGTTGA CTCTGGGGCT
    GACAAAAAGT TTGCCCGGAA GCCACCCCGA TTTTCCTGTC TCCTGCCAGA TCCACGGGAA
    CTAATTGAAA TTAAAAACAA AAAGAAGCTG CTAATCACTG GCACAGAGCA GTTCAATCAG
    AAACCAAAGA AGGGGATTCA GTTTCTGCAA GAGAAAGGCC TCCTCACCAT CCCAATGGAC
    AACACAGAGG TTGCTCAGTG GCTCCGAGAG AACCCTCGGC TGGACAAGAA GATGATTGGA
    GAGTTTGTGA GTGACCGCAA AAACATTGAC CTGTTGGAGA GCTTTGTGAG CACCTTCAGT
    TTTCAGGGTC TGCGACTGGA CGAAGCCCTC CGCCTCTACC TGGAAGCCTT CCGTTTGCCT
    GGGGAAGCAC CAGTCATCCA GAGGTTGCTG GAGGCATTCA CAGAGCGTTG GATGAATTGT
    AATGGCTCCC CATTTGCCAA TAGCGATGCC TGCTTTTCCC TGGCCTATGC TGTCATCATG
    CTTAATACTG ACCAGCACAA CCACAATGTT CGTAAACAGA ATGCACCCAT GACCCTGGAG
    GAGTTTCGCA AAAATCTGAA AGGTGTGAAT GGAGGCAAGG ACTTTGAGCA AGACATCCTG
    GAGGACATGT ACCATGCCAT CAAGAATGAG GAAATTGTAA TGCCTGAGGA GCAGACAGGC
    TTGGTTCGGG AGAACTATGT GTGGAATGTG CTGCTTCATC GAGGTGCCAC CCCTGAGGGC
    ATATTCCTGC GTGTGCCTAC TGCCAGCTAT GATCTTGACC TCTTCACCAT GACCTGGGGC
    CCCACTATTG CTGCTCTCTC TTATGTCTTT GACAAAAGCC TTGAGGAGAC AATCATCCAG
    AAAGCCATCT CAGGCTTCAG GAAGTGCGCC ATGATCTCCG CCCACTATGG CCTCAGCGAT
    GTGTTTGACA ATCTCATCAT CTCTCTATGC AAATTCACAG CTCTCAGCAG TGAGTCTATT
    GAGAACCTGC CCAGTGTATT TGGAAGCAAC CCTAAAGCCC ATATTGCAGC CAAGACAGTA
    TTCCATTTGG CCCATCGTCA TGGTGACATC CTGCGGGAGG GCTGGAAGAA TATCATGGAG
    GCCATGCTGC AGCTCTTCCG AGCCCAACTA CTGCCCAAGG CTATGATAGA GGTAGAAGAT
    TTCGTGGATC CCAATGGCAA GATCTCTCTA CAGCGGGAAG AGACACCATC AAACCGAGGA
    GAGTCAACAG TGCTGAGCTT TGTGAGCTGG CTAACACTGA GTGGTCCTGA GCAGTCTAGT
    GTTCGGGGCC CATCCACTGA AAACCAAGAG GCCAAGAGAG TGGCCTTAGA GTGTATAAAG
    CAATGTGACC CAGAAAAAAT GATCACAGAA AGCAAGTTCC TCCAGCTGGA GTCACTACAG
    GAGCTCATGA AGGCTCTGGT CTCAGTGACA CCAGATGAAG AGACATATGA TGAGGAAGAT
    GCTGCTTTCT GCCTAGAGAT GCTGCTAAGG ATTGTGTTGG AGAACAGGGA TCGTGTGGGC
    TGTGTGTGGC AGACTGTTCG AGACCATCTA TACCACCTCT GTGTTCAGGC ACAAGATTTC
    TGCTTCCTTG TGGAGCGGGC AGTGGTGGGG TTGCTACGCC TGGCCATTCG GCTTCTCCGG
    AGAGAAGAGA TCAGTGCTCA GGTGCTGCTC TCCCTGCGCA TTTTGCTACT GATGAAGCCC
    AGTGTGCTAT CCCGAGTCAG CCACCAGGTT GCGTATGGGC TCCATGAACT CCTGAAGACC
    AATGCAGCCA ACATCCACTC AGGTGATGAC TGGGCCACAC TCTTCACACT GCTGGAGTGC
    ATCGGCTCAG GTGTGAAGCC TCCAGCTGCT CTGCAGGCCA CAGCCAGGGC AGATGCACCT
    GATGCCGGGG CCCAGTCAGA TAGTGAGCTC CCATCCTACC ATCAGAATGA CGTGAGCCTG
    GATCGAGGGT ACACTTCCGA CTCAGAGGTC TACACTGACC ATGGCAGGCC GGGCAAGATA
    CACCGATCAG CCACAGATGC CGATGTGGTC AACAGTGGTT GGTTAGTGGT TGGGAAGGAT
    GACGTTGATA ACTCCAAGCC AGGGCCCAGC CGCCCAGGCC CTTCACCCCT GATCAATCAA
    TACAGCCTAA CAGTGGGACT GGATTTGGGG CCACACGACA CTAAGTCTCT GCTTAAGTGT
    GTGGAATCGC TGTCCTTCAT TGTGCGTGAT GCTGCCCACA TCACACCTGA CAACTTTGAG
    CTCTGCGTCA AGACTCTCCG GATCTTTGTG GAGGCCAGTC TGAATGGCGG ATGCAAGTCC
    CAGGAGAAAC GTGGCAAGAG TCACAAATAT GACAGCAAAG GGAACCGCTT CAAGAAGAAA
    TCCAAAGAGG GATCAATGCT TCGCCGGCCT CGAACCTCCA GCCAACATGC CTCTCGGGGC
    GGGCAGAGTG ATGATGATGA GGACGAAGGC GTGCCTGCCA GCTACCATAC GGTGTCTTTA
    CAGGTCAGTC AGGACTTGCT AGACCTGATG CACACCCTGC ACACGCGGGC AGCCTCTATC
    TACAGCTCAT GGGCGGAGGA GCAACGCCAC CTGGAGACAG GTGGCCAGAA GATTGAAGCT
    GATTCTCGCA CCCTCTGGGC CCACTGCTGG TGCCCTTTAC TGCAGGGTAT TGCCTGCCTG
    TGCTGCGATG CCCGGCGCCA GGTACGGATG CAGGCACTGA CCTATCTGCA GCGAGCACTA
    CTTGTACATG ATCTGCAAAA GCTAGATGCC CTGGAATGGG AGTCCTGTTT TAACAAGGTG
    CTGTTTCCTC TACTTACCAA GCTCTTGGAG AACATCAGCC CTGCAGATGT GGGTGGGATG
    GAGGAGACCC GGATGAGGGC TTCCACATTG CTCTCTAAGG TCTTCCTGCA GCACCTGTCT
    CCACTGCTGT CACTCTCTAC CTTTGCGGCC CTCTGGCTCA CCATCTTGGA CTTCATGGAC
    AAGTACATGC ACGCAGGCTC CAGCGACTTA CTGTCAGAGG CGATCCCTGA GTCTCTGAAG
    AACATGCTTC TGGTGATGGA CACAGCGGAG ATTTTCCACA GTGCAGATGC ACGGGGAGGC
    GGCCCCTCGG CCCTCTGGGA GATCACCTGG GAACGCATTG ACTGTTTTCT CCCTCACCTA
    CGAGATGAAC TCTTCAAGCA GACCGTCATC CAGGACCCCA TGCCCATGGA GCCTCAAGGC
    CAAAAGCCTC TCGCCTCAGC CCACCTGACT TCCGCTGCTG GCGACACTAG GACACCTGGC
    CATCCACCGC CCCCAGAGAT TCCATCTGAG CTGGGGGCCT GTGACTTTGA GAAGCCCGAG
    AGCCCCCGAG CCGCCAGCAG CAGCTCCCCA GGATCACCAG TGGCCTCAAG CCCCAGCAGG
    CTGAGCCCCA CCCCCGACGG GCCTCCACCC TTGGCTCAGC CCCCACTGAT CCTGCAGCCC
    TTGGCCTCCC CACTGCAGGT GGGCGTGCCA CCTATGACTC TGCCCATCAT CCTCAACCCT
    GCGCTCATCG AGGCCACCTC ACCAGTGCCC CTCCTGGCCA CACCCCGCCC CACAGATCCC
    ATACCCACCT CTGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06003
    Clone ID Related Accession (Same CDS sequence) NM_001199378.1
    Accession Version NM_001199378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, AK025330.1, AF068755.1, BC007941.2 and AI859700.1. Transcript Variant: This variant (2) uses multiple alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK025330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATC
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186307.1
    CDS295..5865
    Translation

    Target ORF information:

    RefSeq Version NM_001199378.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATC
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11757
    Clone ID Related Accession (Same CDS sequence) NM_001199379.1
    Accession Version NM_001199379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, BC117681.1, AL121928.13, BC007941.2 and AI859700.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.181248.1, SRR1660809.139641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC
    CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG
    ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT
    TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA
    CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA
    TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC
    AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT
    AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG
    CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA
    GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC
    CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC
    ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC
    CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT
    GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG
    GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA
    CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT
    CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG
    ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG
    GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186308.1
    CDS295..5862
    Translation

    Target ORF information:

    RefSeq Version NM_001199379.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC
    CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG
    ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT
    TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA
    CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA
    TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC
    AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT
    AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG
    CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA
    GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC
    CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC
    ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC
    CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT
    GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG
    GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA
    CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT
    CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG
    ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG
    GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu55708
    Clone ID Related Accession (Same CDS sequence) NM_004193.2
    Accession Version NM_004193.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA772398.1, DA176540.1, AF068755.1 and AI859700.1. On Dec 8, 2010 this sequence version replaced NM_004193.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.173865.1, SRR1660803.8648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004184.1
    CDS295..5874
    Translation

    Target ORF information:

    RefSeq Version NM_004193.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004193.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC
    CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC
    ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT
    CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC
    CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA
    GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC
    TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC
    TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA
    AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCGC ATCCCCAGCT TTGTCACAGA GCTCTACATC
    AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC
    CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC
    ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA
    GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC
    ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA
    TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA
    CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT
    CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA
    GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC
    AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC
    AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT
    CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA
    AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC
    TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG
    AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC
    CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC
    ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG
    CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC
    CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG
    CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC
    CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT
    GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA
    GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT
    GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA
    GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT
    GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG
    CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT
    TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG
    CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC
    TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC
    AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC
    CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC
    GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGG TCAGTCAGGA CTTGCTAGAC
    CTGATGCACA CCCTGCACAC GCGGGCAGCC TCTATCTACA GCTCATGGGC GGAGGAGCAA
    CGCCACCTGG AGACAGGTGG CCAGAAGATT GAAGCTGATT CTCGCACCCT CTGGGCCCAC
    TGCTGGTGCC CTTTACTGCA GGGTATTGCC TGCCTGTGCT GCGATGCCCG GCGCCAGGTA
    CGGATGCAGG CACTGACCTA TCTGCAGCGA GCACTACTTG TACATGATCT GCAAAAGCTA
    GATGCCCTGG AATGGGAGTC CTGTTTTAAC AAGGTGCTGT TTCCTCTACT TACCAAGCTC
    TTGGAGAACA TCAGCCCTGC AGATGTGGGT GGGATGGAGG AGACCCGGAT GAGGGCTTCC
    ACATTGCTCT CTAAGGTCTT CCTGCAGCAC CTGTCTCCAC TGCTGTCACT CTCTACCTTT
    GCGGCCCTCT GGCTCACCAT CTTGGACTTC ATGGACAAGT ACATGCACGC AGGCTCCAGC
    GACTTACTGT CAGAGGCGAT CCCTGAGTCT CTGAAGAACA TGCTTCTGGT GATGGACACA
    GCGGAGATTT TCCACAGTGC AGATGCACGG GGAGGCGGCC CCTCGGCCCT CTGGGAGATC
    ACCTGGGAAC GCATTGACTG TTTTCTCCCT CACCTACGAG ATGAACTCTT CAAGCAGACC
    GTCATCCAGG ACCCCATGCC CATGGAGCCT CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC
    CTGACTTCCG CTGCTGGCGA CACTAGGACA CCTGGCCATC CACCGCCCCC AGAGATTCCA
    TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC
    TCCCCAGGAT CACCAGTGGC CTCAAGCCCC AGCAGGCTGA GCCCCACCCC CGACGGGCCT
    CCACCCTTGG CTCAGCCCCC ACTGATCCTG CAGCCCTTGG CCTCCCCACT GCAGGTGGGC
    GTGCCACCTA TGACTCTGCC CATCATCCTC AACCCTGCGC TCATCGAGGC CACCTCACCA
    GTGCCCCTCC TGGCCACACC CCGCCCCACA GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44653
    Clone ID Related Accession (Same CDS sequence) XM_006718047.2 , XM_006718047.1
    Accession Version XM_006718047.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC
    ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG
    ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT
    GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC
    TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG
    CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC
    GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT
    GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG
    GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG
    CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718110.1
    CDS262..5943
    Translation

    Target ORF information:

    RefSeq Version XM_006718047.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718047.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC
    ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG
    ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT
    GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC
    TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG
    CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC
    GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT
    GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG
    GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG
    CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44653
    Clone ID Related Accession (Same CDS sequence) XM_006718047.2 , XM_006718047.1
    Accession Version XM_006718047.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC
    ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG
    ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT
    GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC
    TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG
    CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC
    GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT
    GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG
    GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG
    CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718110.1
    CDS295..5976
    Translation

    Target ORF information:

    RefSeq Version XM_006718047.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTTACCAAGC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACCCGG ATGAGGGCTT CCACATTGCT CTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTCACC
    ATCTTGGACT TCATGGACAA GTACATGCAC GCAGGCTCCA GCGACTTACT GTCAGAGGCG
    ATCCCTGAGT CTCTGAAGAA CATGCTTCTG GTGATGGACA CAGCGGAGAT TTTCCACAGT
    GCAGATGCAC GGGGAGGCGG CCCCTCGGCC CTCTGGGAGA TCACCTGGGA ACGCATTGAC
    TGTTTTCTCC CTCACCTACG AGATGAACTC TTCAAGCAGA CCGTCATCCA GGACCCCATG
    CCCATGGAGC CTCAAGGCCA AAAGCCTCTC GCCTCAGCCC ACCTGACTTC CGCTGCTGGC
    GACACTAGGA CACCTGGCCA TCCACCGCCC CCAGAGATTC CATCTGAGCT GGGGGCCTGT
    GACTTTGAGA AGCCCGAGAG CCCCCGAGCC GCCAGCAGCA GCTCCCCAGG ATCACCAGTG
    GCCTCAAGCC CCAGCAGGCT GAGCCCCACC CCCGACGGGC CTCCACCCTT GGCTCAGCCC
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA CTGCAGGTGG GCGTGCCACC TATGACTCTG
    CCCATCATCC TCAACCCTGC GCTCATCGAG GCCACCTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGATCCCAT ACCCACCTCT GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44654
    Clone ID Related Accession (Same CDS sequence) XM_006718048.1 , XM_006718048.2
    Accession Version XM_006718048.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA
    GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT
    CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG
    ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA
    GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG
    CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT
    GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC
    TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC
    ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG
    ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA
    ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA
    GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT
    GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA
    GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC
    TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA
    CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT
    GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT
    GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC
    ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG
    GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC
    AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG
    ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA
    CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT
    CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC
    TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG
    CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA
    TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC
    AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT
    CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC
    ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG
    CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT
    GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC
    ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC
    AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG
    AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC
    AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA
    GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA
    CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG
    TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA
    CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT
    GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC
    CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA
    CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC
    CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG
    CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC
    CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG
    GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT
    CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC
    TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA
    GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC
    CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC
    TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC
    CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA
    GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT
    TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC
    ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC
    ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC
    TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC
    TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA
    CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC
    ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC
    CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718111.1
    CDS295..5973
    Translation

    Target ORF information:

    RefSeq Version XM_006718048.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA
    GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT
    CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG
    ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA
    GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG
    CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT
    GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC
    TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC
    ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG
    ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA
    ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA
    GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT
    GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA
    GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC
    TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA
    CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT
    GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT
    GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC
    ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG
    GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC
    AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG
    ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA
    CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT
    CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC
    TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG
    CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA
    TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC
    AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT
    CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC
    ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG
    CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT
    GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC
    ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC
    AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG
    AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC
    AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA
    GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA
    CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG
    TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA
    CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT
    GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC
    CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA
    CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC
    CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG
    CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC
    CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG
    GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT
    CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC
    TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA
    GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC
    CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC
    TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC
    CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA
    GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT
    TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC
    ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC
    ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC
    TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC
    TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA
    CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC
    ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC
    CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44654
    Clone ID Related Accession (Same CDS sequence) XM_006718048.1 , XM_006718048.2
    Accession Version XM_006718048.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA
    GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT
    CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG
    ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA
    GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG
    CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT
    GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC
    TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC
    ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG
    ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA
    ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA
    GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT
    GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA
    GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC
    TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA
    CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT
    GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT
    GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC
    ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG
    GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC
    AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG
    ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA
    CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT
    CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC
    TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG
    CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA
    TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC
    AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT
    CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC
    ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG
    CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT
    GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC
    ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC
    AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG
    AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC
    AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA
    GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA
    CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG
    TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA
    CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT
    GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC
    CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA
    CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC
    CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG
    CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC
    CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG
    GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT
    CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC
    TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA
    GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC
    CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC
    TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC
    CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA
    GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT
    TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC
    ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC
    ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC
    TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC
    TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA
    CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC
    ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC
    CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718111.1
    CDS262..5940
    Translation

    Target ORF information:

    RefSeq Version XM_006718048.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718048.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG GAAGGGACCC ATGTGGAAAA GTCCCAGTCA
    GCATCTGTGG AGTCCATCCC TGAAGTGTTA GAGGAGTGCA CGTCCCCTGC CGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGCGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGCAC AGCTTTGGTC CCCTATGGTC TTCCCTGCAT CCGCGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CACGACCGCC ATAACTCAGA GGTTATGATT
    CACATGGGAC TGCATTTGCT GACAGTGGCC CTTGAGTCAG CCCCTGTAGC CCAGTGCCAG
    ACCCTCTTGG GCCTCATCAA GGATGAGATG TGCCGTCACT TATTCCAGCT ACTCAGCATA
    GAGCGACTAA ACCTTTATGC TGCTTCCCTG CGAGTATGCT TCCTACTGTT TGAGAGCATG
    CGAGAGCACC TCAAGTTCCA AATGGAGATG TACATCAAAA AGCTTATGGA GATCATCACT
    GTGGAGAACC CCAAGATGCC TTATGAGATG AAGGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCGCA TCCCCAGCTT TGTCACAGAG CTCTACATCA ACTATGATTG TGACTACTAC
    TGTTCCAACC TCTTTGAGGA ACTCACAAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAACTCT ATACAACACA CCTACTATCT CTTGATGCCC TATTGACAGT GATTGACAGC
    ACCGAGGCCC ACTGCCAGGC TAAAGTCCTC AACAGCCTCA CCCAGCAAGA GAAGAAGGAG
    ACAGCCAGAC CAAGCTGTGA GATAGTAGAT GGCACCCGAG AAGCTAGCAA TACTGAGAGA
    ACTGCCAGCG ATGGGAAAGC TGTAGGCATG GCCTCAGACA TCCCAGGCCT GCATCTGCCA
    GGTGGAGGGC GGCTGCCACC AGAACATGGG AAATCAGGAT GCAGTGATCT GGAGGAAGCT
    GTTGACTCTG GGGCTGACAA AAAGTTTGCC CGGAAGCCAC CCCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTAAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ATCAGAAACC AAAGAAGGGG ATTCAGTTTC TGCAAGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTTGCT CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAGATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGTTTTCA GGGTCTGCGA CTGGACGAAG CCCTCCGCCT CTACCTGGAA
    GCCTTCCGTT TGCCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CGTTGGATGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TTCCCTGGCC
    TATGCTGTCA TCATGCTTAA TACTGACCAG CACAACCACA ATGTTCGTAA ACAGAATGCA
    CCCATGACCC TGGAGGAGTT TCGCAAAAAT CTGAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTAATGCCT
    GAGGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTGCT TCATCGAGGT
    GCCACCCCTG AGGGCATATT CCTGCGTGTG CCTACTGCCA GCTATGATCT TGACCTCTTC
    ACCATGACCT GGGGCCCCAC TATTGCTGCT CTCTCTTATG TCTTTGACAA AAGCCTTGAG
    GAGACAATCA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATCTCTC TATGCAAATT CACAGCTCTC
    AGCAGTGAGT CTATTGAGAA CCTGCCCAGT GTATTTGGAA GCAACCCTAA AGCCCATATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ACATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAGGCCAT GCTGCAGCTC TTCCGAGCCC AACTACTGCC CAAGGCTATG
    ATAGAGGTAG AAGATTTCGT GGATCCCAAT GGCAAGATCT CTCTACAGCG GGAAGAGACA
    CCATCAAACC GAGGAGAGTC AACAGTGCTG AGCTTTGTGA GCTGGCTAAC ACTGAGTGGT
    CCTGAGCAGT CTAGTGTTCG GGGCCCATCC ACTGAAAACC AAGAGGCCAA GAGAGTGGCC
    TTAGAGTGTA TAAAGCAATG TGACCCAGAA AAAATGATCA CAGAAAGCAA GTTCCTCCAG
    CTGGAGTCAC TACAGGAGCT CATGAAGGCT CTGGTCTCAG TGACACCAGA TGAAGAGACA
    TATGATGAGG AAGATGCTGC TTTCTGCCTA GAGATGCTGC TAAGGATTGT GTTGGAGAAC
    AGGGATCGTG TGGGCTGTGT GTGGCAGACT GTTCGAGACC ATCTATACCA CCTCTGTGTT
    CAGGCACAAG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGTTGCT ACGCCTGGCC
    ATTCGGCTTC TCCGGAGAGA AGAGATCAGT GCTCAGGTGC TGCTCTCCCT GCGCATTTTG
    CTACTGATGA AGCCCAGTGT GCTATCCCGA GTCAGCCACC AGGTTGCGTA TGGGCTCCAT
    GAACTCCTGA AGACCAATGC AGCCAACATC CACTCAGGTG ATGACTGGGC CACACTCTTC
    ACACTGCTGG AGTGCATCGG CTCAGGTGTG AAGCCTCCAG CTGCTCTGCA GGCCACAGCC
    AGGGCAGATG CACCTGATGC CGGGGCCCAG TCAGATAGTG AGCTCCCATC CTACCATCAG
    AATGACGTGA GCCTGGATCG AGGGTACACT TCCGACTCAG AGGTCTACAC TGACCATGGC
    AGGCCGGGCA AGATACACCG ATCAGCCACA GATGCCGATG TGGTCAACAG TGGTTGGTTA
    GTGGTTGGGA AGGATGACGT TGATAACTCC AAGCCAGGGC CCAGCCGCCC AGGCCCTTCA
    CCCCTGATCA ATCAATACAG CCTAACAGTG GGACTGGATT TGGGGCCACA CGACACTAAG
    TCTCTGCTTA AGTGTGTGGA ATCGCTGTCC TTCATTGTGC GTGATGCTGC CCACATCACA
    CCTGACAACT TTGAGCTCTG CGTCAAGACT CTCCGGATCT TTGTGGAGGC CAGTCTGAAT
    GGCGGATGCA AGTCCCAGGA GAAACGTGGC AAGAGTCACA AATATGACAG CAAAGGGAAC
    CGCTTCAAGA AGAAATCCAA AGAGGGATCA ATGCTTCGCC GGCCTCGAAC CTCCAGCCAA
    CATGCCTCTC GGGGCGGGCA GAGTGATGAT GATGAGGACG AAGGCGTGCC TGCCAGCTAC
    CATACGGTGT CTTTACAGGT CAGTCAGGAC TTGCTAGACC TGATGCACAC CCTGCACACG
    CGGGCAGCCT CTATCTACAG CTCATGGGCG GAGGAGCAAC GCCACCTGGA GACAGGTGGC
    CAGAAGATTG AAGCTGATTC TCGCACCCTC TGGGCCCACT GCTGGTGCCC TTTACTGCAG
    GGTATTGCCT GCCTGTGCTG CGATGCCCGG CGCCAGGTAC GGATGCAGGC ACTGACCTAT
    CTGCAGCGAG CACTACTTGT ACATGATCTG CAAAAGCTAG ATGCCCTGGA ATGGGAGTCC
    TGTTTTAACA AGGTGCTGTT TCCTCTACTT ACCAAGCTCT TGGAGAACAT CAGCCCTGCA
    GATGTGGGTG GGATGGAGGA GACCCGGATG AGGGCTTCCA CATTGCTCTC TAAGGTCTTC
    CTGCAGCACC TGTCTCCACT GCTGTCACTC TCTACCTTTG CGGCCCTCTG GCTCACCATC
    TTGGACTTCA TGGACAAGTA CATGCACGCA GGCTCCAGCG ACTTACTGTC AGAGGCGATC
    CCTGAGTCTC TGAAGAACAT GCTTCTGGTG ATGGACACAG CGGAGATTTT CCACAGTGCA
    GATGCACGGG GAGGCGGCCC CTCGGCCCTC TGGGAGATCA CCTGGGAACG CATTGACTGT
    TTTCTCCCTC ACCTACGAGA TGAACTCTTC AAGCAGACCG TCATCCAGGA CCCCATGCCC
    ATGGAGCCTC AAGGCCAAAA GCCTCTCGCC TCAGCCCACC TGACTTCCGC TGCTGGCGAC
    ACTAGGACAC CTGGCCATCC ACCGCCCCCA GAGATTCCAT CTGAGCTGGG GGCCTGTGAC
    TTTGAGAAGC CCGAGAGCCC CCGAGCCGCC AGCAGCAGCT CCCCAGGATC ACCAGTGGCC
    TCAAGCCCCA GCAGGCTGAG CCCCACCCCC GACGGGCCTC CACCCTTGGC TCAGCCCCCA
    CTGATCCTGC AGCCCTTGGC CTCCCCACTG CAGGTGGGCG TGCCACCTAT GACTCTGCCC
    ATCATCCTCA ACCCTGCGCT CATCGAGGCC ACCTCACCAG TGCCCCTCCT GGCCACACCC
    CGCCCCACAG ATCCCATACC CACCTCTGAG GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44655
    Clone ID Related Accession (Same CDS sequence) XM_006718049.1 , XM_006718049.2
    Accession Version XM_006718049.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC
    TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT
    GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC
    TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA
    GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC
    AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT
    GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC
    CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC
    ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT
    CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG
    GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT
    CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT
    CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA
    CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG
    CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC
    AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG
    CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC
    AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA
    GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718112.1
    CDS295..5964
    Translation

    Target ORF information:

    RefSeq Version XM_006718049.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC
    TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT
    GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC
    TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA
    GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC
    AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT
    GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC
    CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC
    ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT
    CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG
    GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT
    CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT
    CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA
    CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG
    CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC
    AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG
    CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC
    AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA
    GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44655
    Clone ID Related Accession (Same CDS sequence) XM_006718049.1 , XM_006718049.2
    Accession Version XM_006718049.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC
    TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT
    GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC
    TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA
    GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC
    AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT
    GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC
    CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC
    ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT
    CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG
    GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT
    CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT
    CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA
    CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG
    CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC
    AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG
    CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC
    AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA
    GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718112.1
    CDS262..5931
    Translation

    Target ORF information:

    RefSeq Version XM_006718049.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718049.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GTTGCTAGAC CTGATGCACA CCCTGCACAC GCGGGCAGCC
    TCTATCTACA GCTCATGGGC GGAGGAGCAA CGCCACCTGG AGACAGGTGG CCAGAAGATT
    GAAGCTGATT CTCGCACCCT CTGGGCCCAC TGCTGGTGCC CTTTACTGCA GGGTATTGCC
    TGCCTGTGCT GCGATGCCCG GCGCCAGGTA CGGATGCAGG CACTGACCTA TCTGCAGCGA
    GCACTACTTG TACATGATCT GCAAAAGCTA GATGCCCTGG AATGGGAGTC CTGTTTTAAC
    AAGGTGCTGT TTCCTCTACT TACCAAGCTC TTGGAGAACA TCAGCCCTGC AGATGTGGGT
    GGGATGGAGG AGACCCGGAT GAGGGCTTCC ACATTGCTCT CTAAGGTCTT CCTGCAGCAC
    CTGTCTCCAC TGCTGTCACT CTCTACCTTT GCGGCCCTCT GGCTCACCAT CTTGGACTTC
    ATGGACAAGT ACATGCACGC AGGCTCCAGC GACTTACTGT CAGAGGCGAT CCCTGAGTCT
    CTGAAGAACA TGCTTCTGGT GATGGACACA GCGGAGATTT TCCACAGTGC AGATGCACGG
    GGAGGCGGCC CCTCGGCCCT CTGGGAGATC ACCTGGGAAC GCATTGACTG TTTTCTCCCT
    CACCTACGAG ATGAACTCTT CAAGCAGACC GTCATCCAGG ACCCCATGCC CATGGAGCCT
    CAAGGCCAAA AGCCTCTCGC CTCAGCCCAC CTGACTTCCG CTGCTGGCGA CACTAGGACA
    CCTGGCCATC CACCGCCCCC AGAGATTCCA TCTGAGCTGG GGGCCTGTGA CTTTGAGAAG
    CCCGAGAGCC CCCGAGCCGC CAGCAGCAGC TCCCCAGGAT CACCAGTGGC CTCAAGCCCC
    AGCAGGCTGA GCCCCACCCC CGACGGGCCT CCACCCTTGG CTCAGCCCCC ACTGATCCTG
    CAGCCCTTGG CCTCCCCACT GCAGGTGGGC GTGCCACCTA TGACTCTGCC CATCATCCTC
    AACCCTGCGC TCATCGAGGC CACCTCACCA GTGCCCCTCC TGGCCACACC CCGCCCCACA
    GATCCCATAC CCACCTCTGA GGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44656
    Clone ID Related Accession (Same CDS sequence) XM_006718050.1 , XM_006718050.2
    Accession Version XM_006718050.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718113.1
    CDS295..5940
    Translation

    Target ORF information:

    RefSeq Version XM_006718050.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44656
    Clone ID Related Accession (Same CDS sequence) XM_006718050.1 , XM_006718050.2
    Accession Version XM_006718050.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718113.1
    CDS262..5907
    Translation

    Target ORF information:

    RefSeq Version XM_006718050.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTC TGAGAGAACT GCCAGCGATG GGAAAGCTGT AGGCATGGCC
    TCAGACATCC CAGGCCTGCA TCTGCCAGGT GGAGGGCGGC TGCCACCAGA ACATGGGAAA
    TCAGGATGCA GTGATCTGGA GGAAGCTGTT GACTCTGGGG CTGACAAAAA GTTTGCCCGG
    AAGCCACCCC GATTTTCCTG TCTCCTGCCA GATCCACGGG AACTAATTGA AATTAAAAAC
    AAAAAGAAGC TGCTAATCAC TGGCACAGAG CAGTTCAATC AGAAACCAAA GAAGGGGATT
    CAGTTTCTGC AAGAGAAAGG CCTCCTCACC ATCCCAATGG ACAACACAGA GGTTGCTCAG
    TGGCTCCGAG AGAACCCTCG GCTGGACAAG AAGATGATTG GAGAGTTTGT GAGTGACCGC
    AAAAACATTG ACCTGTTGGA GAGCTTTGTG AGCACCTTCA GTTTTCAGGG TCTGCGACTG
    GACGAAGCCC TCCGCCTCTA CCTGGAAGCC TTCCGTTTGC CTGGGGAAGC ACCAGTCATC
    CAGAGGTTGC TGGAGGCATT CACAGAGCGT TGGATGAATT GTAATGGCTC CCCATTTGCC
    AATAGCGATG CCTGCTTTTC CCTGGCCTAT GCTGTCATCA TGCTTAATAC TGACCAGCAC
    AACCACAATG TTCGTAAACA GAATGCACCC ATGACCCTGG AGGAGTTTCG CAAAAATCTG
    AAAGGTGTGA ATGGAGGCAA GGACTTTGAG CAAGACATCC TGGAGGACAT GTACCATGCC
    ATCAAGAATG AGGAAATTGT AATGCCTGAG GAGCAGACAG GCTTGGTTCG GGAGAACTAT
    GTGTGGAATG TGCTGCTTCA TCGAGGTGCC ACCCCTGAGG GCATATTCCT GCGTGTGCCT
    ACTGCCAGCT ATGATCTTGA CCTCTTCACC ATGACCTGGG GCCCCACTAT TGCTGCTCTC
    TCTTATGTCT TTGACAAAAG CCTTGAGGAG ACAATCATCC AGAAAGCCAT CTCAGGCTTC
    AGGAAGTGCG CCATGATCTC CGCCCACTAT GGCCTCAGCG ATGTGTTTGA CAATCTCATC
    ATCTCTCTAT GCAAATTCAC AGCTCTCAGC AGTGAGTCTA TTGAGAACCT GCCCAGTGTA
    TTTGGAAGCA ACCCTAAAGC CCATATTGCA GCCAAGACAG TATTCCATTT GGCCCATCGT
    CATGGTGACA TCCTGCGGGA GGGCTGGAAG AATATCATGG AGGCCATGCT GCAGCTCTTC
    CGAGCCCAAC TACTGCCCAA GGCTATGATA GAGGTAGAAG ATTTCGTGGA TCCCAATGGC
    AAGATCTCTC TACAGCGGGA AGAGACACCA TCAAACCGAG GAGAGTCAAC AGTGCTGAGC
    TTTGTGAGCT GGCTAACACT GAGTGGTCCT GAGCAGTCTA GTGTTCGGGG CCCATCCACT
    GAAAACCAAG AGGCCAAGAG AGTGGCCTTA GAGTGTATAA AGCAATGTGA CCCAGAAAAA
    ATGATCACAG AAAGCAAGTT CCTCCAGCTG GAGTCACTAC AGGAGCTCAT GAAGGCTCTG
    GTCTCAGTGA CACCAGATGA AGAGACATAT GATGAGGAAG ATGCTGCTTT CTGCCTAGAG
    ATGCTGCTAA GGATTGTGTT GGAGAACAGG GATCGTGTGG GCTGTGTGTG GCAGACTGTT
    CGAGACCATC TATACCACCT CTGTGTTCAG GCACAAGATT TCTGCTTCCT TGTGGAGCGG
    GCAGTGGTGG GGTTGCTACG CCTGGCCATT CGGCTTCTCC GGAGAGAAGA GATCAGTGCT
    CAGGTGCTGC TCTCCCTGCG CATTTTGCTA CTGATGAAGC CCAGTGTGCT ATCCCGAGTC
    AGCCACCAGG TTGCGTATGG GCTCCATGAA CTCCTGAAGA CCAATGCAGC CAACATCCAC
    TCAGGTGATG ACTGGGCCAC ACTCTTCACA CTGCTGGAGT GCATCGGCTC AGGTGTGAAG
    CCTCCAGCTG CTCTGCAGGC CACAGCCAGG GCAGATGCAC CTGATGCCGG GGCCCAGTCA
    GATAGTGAGC TCCCATCCTA CCATCAGAAT GACGTGAGCC TGGATCGAGG GTACACTTCC
    GACTCAGAGG TCTACACTGA CCATGGCAGG CCGGGCAAGA TACACCGATC AGCCACAGAT
    GCCGATGTGG TCAACAGTGG TTGGTTAGTG GTTGGGAAGG ATGACGTTGA TAACTCCAAG
    CCAGGGCCCA GCCGCCCAGG CCCTTCACCC CTGATCAATC AATACAGCCT AACAGTGGGA
    CTGGATTTGG GGCCACACGA CACTAAGTCT CTGCTTAAGT GTGTGGAATC GCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATCACACCT GACAACTTTG AGCTCTGCGT CAAGACTCTC
    CGGATCTTTG TGGAGGCCAG TCTGAATGGC GGATGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGGAACCGC TTCAAGAAGA AATCCAAAGA GGGATCAATG
    CTTCGCCGGC CTCGAACCTC CAGCCAACAT GCCTCTCGGG GCGGGCAGAG TGATGATGAT
    GAGGACGAAG GCGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    CTAGACCTGA TGCACACCCT GCACACGCGG GCAGCCTCTA TCTACAGCTC ATGGGCGGAG
    GAGCAACGCC ACCTGGAGAC AGGTGGCCAG AAGATTGAAG CTGATTCTCG CACCCTCTGG
    GCCCACTGCT GGTGCCCTTT ACTGCAGGGT ATTGCCTGCC TGTGCTGCGA TGCCCGGCGC
    CAGGTACGGA TGCAGGCACT GACCTATCTG CAGCGAGCAC TACTTGTACA TGATCTGCAA
    AAGCTAGATG CCCTGGAATG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTTACC
    AAGCTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC CCGGATGAGG
    GCTTCCACAT TGCTCTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT CACCATCTTG GACTTCATGG ACAAGTACAT GCACGCAGGC
    TCCAGCGACT TACTGTCAGA GGCGATCCCT GAGTCTCTGA AGAACATGCT TCTGGTGATG
    GACACAGCGG AGATTTTCCA CAGTGCAGAT GCACGGGGAG GCGGCCCCTC GGCCCTCTGG
    GAGATCACCT GGGAACGCAT TGACTGTTTT CTCCCTCACC TACGAGATGA ACTCTTCAAG
    CAGACCGTCA TCCAGGACCC CATGCCCATG GAGCCTCAAG GCCAAAAGCC TCTCGCCTCA
    GCCCACCTGA CTTCCGCTGC TGGCGACACT AGGACACCTG GCCATCCACC GCCCCCAGAG
    ATTCCATCTG AGCTGGGGGC CTGTGACTTT GAGAAGCCCG AGAGCCCCCG AGCCGCCAGC
    AGCAGCTCCC CAGGATCACC AGTGGCCTCA AGCCCCAGCA GGCTGAGCCC CACCCCCGAC
    GGGCCTCCAC CCTTGGCTCA GCCCCCACTG ATCCTGCAGC CCTTGGCCTC CCCACTGCAG
    GTGGGCGTGC CACCTATGAC TCTGCCCATC ATCCTCAACC CTGCGCTCAT CGAGGCCACC
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGATC CCATACCCAC CTCTGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44657
    Clone ID Related Accession (Same CDS sequence) XM_005270261.1 , NM_001377137.1 , XM_005270261.2
    Accession Version XM_005270261.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005270318.1
    CDS295..5877
    Translation

    Target ORF information:

    RefSeq Version XM_005270261.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005270261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44657
    Clone ID Related Accession (Same CDS sequence) XM_005270261.1 , NM_001377137.1 , XM_005270261.2
    Accession Version XM_005270261.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005270261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005270318.1
    CDS262..5844
    Translation

    Target ORF information:

    RefSeq Version XM_005270261.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005270261.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC
    ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG
    ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC
    CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC
    ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT
    ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GCAGGAAGGG
    ACCCATGTGG AAAAGTCCCA GTCAGCATCT GTGGAGTCCA TCCCTGAAGT GTTAGAGGAG
    TGCACGTCCC CTGCCGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GCACAGCTTT GGTCCCCTAT
    GGTCTTCCCT GCATCCGCGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACACGAC
    CGCCATAACT CAGAGGTTAT GATTCACATG GGACTGCATT TGCTGACAGT GGCCCTTGAG
    TCAGCCCCTG TAGCCCAGTG CCAGACCCTC TTGGGCCTCA TCAAGGATGA GATGTGCCGT
    CACTTATTCC AGCTACTCAG CATAGAGCGA CTAAACCTTT ATGCTGCTTC CCTGCGAGTA
    TGCTTCCTAC TGTTTGAGAG CATGCGAGAG CACCTCAAGT TCCAAATGGA GATGTACATC
    AAAAAGCTTA TGGAGATCAT CACTGTGGAG AACCCCAAGA TGCCTTATGA GATGAAGGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CGCATCCCCA GCTTTGTCAC AGAGCTCTAC
    ATCAACTATG ATTGTGACTA CTACTGTTCC AACCTCTTTG AGGAACTCAC AAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAA CTCTATACAA CACACCTACT ATCTCTTGAT
    GCCCTATTGA CAGTGATTGA CAGCACCGAG GCCCACTGCC AGGCTAAAGT CCTCAACAGC
    CTCACCCAGC AAGAGAAGAA GGAGACAGCC AGACCAAGCT GTGAGATAGT AGATGGCACC
    CGAGAAGCTA GCAATACTGA GAGAACTGCC AGCGATGGGA AAGCTGTAGG CATGGCCTCA
    GACATCCCAG GCCTGCATCT GCCAGGTGGA GGGCGGCTGC CACCAGAACA TGGGAAATCA
    GGATGCAGTG ATCTGGAGGA AGCTGTTGAC TCTGGGGCTG ACAAAAAGTT TGCCCGGAAG
    CCACCCCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TAATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAATCAGA AACCAAAGAA GGGGATTCAG
    TTTCTGCAAG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT TGCTCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAG ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGTT TTCAGGGTCT GCGACTGGAC
    GAAGCCCTCC GCCTCTACCT GGAAGCCTTC CGTTTGCCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCGTTGG ATGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTTCCCT GGCCTATGCT GTCATCATGC TTAATACTGA CCAGCACAAC
    CACAATGTTC GTAAACAGAA TGCACCCATG ACCCTGGAGG AGTTTCGCAA AAATCTGAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTAAT GCCTGAGGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TGCTTCATCG AGGTGCCACC CCTGAGGGCA TATTCCTGCG TGTGCCTACT
    GCCAGCTATG ATCTTGACCT CTTCACCATG ACCTGGGGCC CCACTATTGC TGCTCTCTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAGACA ATCATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATC
    TCTCTATGCA AATTCACAGC TCTCAGCAGT GAGTCTATTG AGAACCTGCC CAGTGTATTT
    GGAAGCAACC CTAAAGCCCA TATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGACATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAGG CCATGCTGCA GCTCTTCCGA
    GCCCAACTAC TGCCCAAGGC TATGATAGAG GTAGAAGATT TCGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGCGGGAAGA GACACCATCA AACCGAGGAG AGTCAACAGT GCTGAGCTTT
    GTGAGCTGGC TAACACTGAG TGGTCCTGAG CAGTCTAGTG TTCGGGGCCC ATCCACTGAA
    AACCAAGAGG CCAAGAGAGT GGCCTTAGAG TGTATAAAGC AATGTGACCC AGAAAAAATG
    ATCACAGAAA GCAAGTTCCT CCAGCTGGAG TCACTACAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACAC CAGATGAAGA GACATATGAT GAGGAAGATG CTGCTTTCTG CCTAGAGATG
    CTGCTAAGGA TTGTGTTGGA GAACAGGGAT CGTGTGGGCT GTGTGTGGCA GACTGTTCGA
    GACCATCTAT ACCACCTCTG TGTTCAGGCA CAAGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGT TGCTACGCCT GGCCATTCGG CTTCTCCGGA GAGAAGAGAT CAGTGCTCAG
    GTGCTGCTCT CCCTGCGCAT TTTGCTACTG ATGAAGCCCA GTGTGCTATC CCGAGTCAGC
    CACCAGGTTG CGTATGGGCT CCATGAACTC CTGAAGACCA ATGCAGCCAA CATCCACTCA
    GGTGATGACT GGGCCACACT CTTCACACTG CTGGAGTGCA TCGGCTCAGG TGTGAAGCCT
    CCAGCTGCTC TGCAGGCCAC AGCCAGGGCA GATGCACCTG ATGCCGGGGC CCAGTCAGAT
    AGTGAGCTCC CATCCTACCA TCAGAATGAC GTGAGCCTGG ATCGAGGGTA CACTTCCGAC
    TCAGAGGTCT ACACTGACCA TGGCAGGCCG GGCAAGATAC ACCGATCAGC CACAGATGCC
    GATGTGGTCA ACAGTGGTTG GTTAGTGGTT GGGAAGGATG ACGTTGATAA CTCCAAGCCA
    GGGCCCAGCC GCCCAGGCCC TTCACCCCTG ATCAATCAAT ACAGCCTAAC AGTGGGACTG
    GATTTGGGGC CACACGACAC TAAGTCTCTG CTTAAGTGTG TGGAATCGCT GTCCTTCATT
    GTGCGTGATG CTGCCCACAT CACACCTGAC AACTTTGAGC TCTGCGTCAA GACTCTCCGG
    ATCTTTGTGG AGGCCAGTCT GAATGGCGGA TGCAAGTCCC AGGAGAAACG TGGCAAGAGT
    CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAAT CCAAAGAGGG ATCAATGCTT
    CGCCGGCCTC GAACCTCCAG CCAACATGCC TCTCGGGGCG GGCAGAGTGA TGATGATGAG
    GACGAAGGCG TGCCTGCCAG CTACCATACG GTGTCTTTAC AGGTCAGTCA GGACTTGCTA
    GACCTGATGC ACACCCTGCA CACGCGGGCA GCCTCTATCT ACAGCTCATG GGCGGAGGAG
    CAACGCCACC TGGAGACAGG TGGCCAGAAG ATTGAAGCTG ATTCTCGCAC CCTCTGGGCC
    CACTGCTGGT GCCCTTTACT GCAGGGTATT GCCTGCCTGT GCTGCGATGC CCGGCGCCAG
    GTACGGATGC AGGCACTGAC CTATCTGCAG CGAGCACTAC TTGTACATGA TCTGCAAAAG
    CTAGATGCCC TGGAATGGGA GTCCTGTTTT AACAAGGTGC TGTTTCCTCT ACTTACCAAG
    CTCTTGGAGA ACATCAGCCC TGCAGATGTG GGTGGGATGG AGGAGACCCG GATGAGGGCT
    TCCACATTGC TCTCTAAGGT CTTCCTGCAG CACCTGTCTC CACTGCTGTC ACTCTCTACC
    TTTGCGGCCC TCTGGCTCAC CATCTTGGAC TTCATGGACA AGTACATGCA CGCAGGCTCC
    AGCGACTTAC TGTCAGAGGC GATCCCTGAG TCTCTGAAGA ACATGCTTCT GGTGATGGAC
    ACAGCGGAGA TTTTCCACAG TGCAGATGCA CGGGGAGGCG GCCCCTCGGC CCTCTGGGAG
    ATCACCTGGG AACGCATTGA CTGTTTTCTC CCTCACCTAC GAGATGAACT CTTCAAGCAG
    ACCGTCATCC AGGACCCCAT GCCCATGGAG CCTCAAGGCC AAAAGCCTCT CGCCTCAGCC
    CACCTGACTT CCGCTGCTGG CGACACTAGG ACACCTGGCC ATCCACCGCC CCCAGAGATT
    CCATCTGAGC TGGGGGCCTG TGACTTTGAG AAGCCCGAGA GCCCCCGAGC CGCCAGCAGC
    AGCTCCCCAG GATCACCAGT GGCCTCAAGC CCCAGCAGGC TGAGCCCCAC CCCCGACGGG
    CCTCCACCCT TGGCTCAGCC CCCACTGATC CTGCAGCCCT TGGCCTCCCC ACTGCAGGTG
    GGCGTGCCAC CTATGACTCT GCCCATCATC CTCAACCCTG CGCTCATCGA GGCCACCTCA
    CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC ACAGATCCCA TACCCACCTC TGAGGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62151
    Clone ID Related Accession (Same CDS sequence) XM_011540312.1 , XM_011540312.2
    Accession Version XM_011540312.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC
    AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA
    ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA
    CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC
    ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG
    TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT
    CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG
    GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT
    GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT
    AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG
    TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG
    GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG
    GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA
    TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC
    ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA
    GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG
    TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG
    AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC
    CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC
    ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC
    GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG
    TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT
    CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA
    TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG
    CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT
    GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT
    GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC
    TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA
    GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT
    GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT
    GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC
    GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT
    GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT
    CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC
    CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC
    GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC
    AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG
    GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC
    TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC
    AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG
    CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC
    AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT
    TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC
    TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT
    GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG
    TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC
    ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC
    CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA
    GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA
    AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT
    CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC
    CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG
    AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG
    CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA
    CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538614.1
    CDS295..5835
    Translation

    Target ORF information:

    RefSeq Version XM_011540312.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC
    AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA
    ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA
    CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC
    ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG
    TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT
    CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG
    GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT
    GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT
    AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG
    TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG
    GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG
    GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA
    TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC
    ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA
    GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG
    TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG
    AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC
    CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC
    ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC
    GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG
    TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT
    CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA
    TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG
    CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT
    GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT
    GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC
    TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA
    GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT
    GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT
    GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC
    GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT
    GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT
    CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC
    CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC
    GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC
    AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG
    GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC
    TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC
    AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG
    CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC
    AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT
    TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC
    TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT
    GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG
    TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC
    ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC
    CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA
    GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA
    AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT
    CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC
    CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG
    AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG
    CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA
    CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62151
    Clone ID Related Accession (Same CDS sequence) XM_011540312.1 , XM_011540312.2
    Accession Version XM_011540312.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC
    AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA
    ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA
    CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC
    ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG
    TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT
    CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG
    GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT
    GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT
    AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG
    TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG
    GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG
    GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA
    TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC
    ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA
    GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG
    TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG
    AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC
    CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC
    ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC
    GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG
    TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT
    CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA
    TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG
    CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT
    GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT
    GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC
    TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA
    GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT
    GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT
    GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC
    GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT
    GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT
    CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC
    CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC
    GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC
    AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG
    GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC
    TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC
    AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG
    CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC
    AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT
    TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC
    TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT
    GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG
    TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC
    ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC
    CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA
    GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA
    AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT
    CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC
    CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG
    AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG
    CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA
    CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538614.1
    CDS262..5802
    Translation

    Target ORF information:

    RefSeq Version XM_011540312.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAC
    AAAAAGTTTG CCCGGAAGCC ACCCCGATTT TCCTGTCTCC TGCCAGATCC ACGGGAACTA
    ATTGAAATTA AAAACAAAAA GAAGCTGCTA ATCACTGGCA CAGAGCAGTT CAATCAGAAA
    CCAAAGAAGG GGATTCAGTT TCTGCAAGAG AAAGGCCTCC TCACCATCCC AATGGACAAC
    ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC CCTCGGCTGG ACAAGAAGAT GATTGGAGAG
    TTTGTGAGTG ACCGCAAAAA CATTGACCTG TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT
    CAGGGTCTGC GACTGGACGA AGCCCTCCGC CTCTACCTGG AAGCCTTCCG TTTGCCTGGG
    GAAGCACCAG TCATCCAGAG GTTGCTGGAG GCATTCACAG AGCGTTGGAT GAATTGTAAT
    GGCTCCCCAT TTGCCAATAG CGATGCCTGC TTTTCCCTGG CCTATGCTGT CATCATGCTT
    AATACTGACC AGCACAACCA CAATGTTCGT AAACAGAATG CACCCATGAC CCTGGAGGAG
    TTTCGCAAAA ATCTGAAAGG TGTGAATGGA GGCAAGGACT TTGAGCAAGA CATCCTGGAG
    GACATGTACC ATGCCATCAA GAATGAGGAA ATTGTAATGC CTGAGGAGCA GACAGGCTTG
    GTTCGGGAGA ACTATGTGTG GAATGTGCTG CTTCATCGAG GTGCCACCCC TGAGGGCATA
    TTCCTGCGTG TGCCTACTGC CAGCTATGAT CTTGACCTCT TCACCATGAC CTGGGGCCCC
    ACTATTGCTG CTCTCTCTTA TGTCTTTGAC AAAAGCCTTG AGGAGACAAT CATCCAGAAA
    GCCATCTCAG GCTTCAGGAA GTGCGCCATG ATCTCCGCCC ACTATGGCCT CAGCGATGTG
    TTTGACAATC TCATCATCTC TCTATGCAAA TTCACAGCTC TCAGCAGTGA GTCTATTGAG
    AACCTGCCCA GTGTATTTGG AAGCAACCCT AAAGCCCATA TTGCAGCCAA GACAGTATTC
    CATTTGGCCC ATCGTCATGG TGACATCCTG CGGGAGGGCT GGAAGAATAT CATGGAGGCC
    ATGCTGCAGC TCTTCCGAGC CCAACTACTG CCCAAGGCTA TGATAGAGGT AGAAGATTTC
    GTGGATCCCA ATGGCAAGAT CTCTCTACAG CGGGAAGAGA CACCATCAAA CCGAGGAGAG
    TCAACAGTGC TGAGCTTTGT GAGCTGGCTA ACACTGAGTG GTCCTGAGCA GTCTAGTGTT
    CGGGGCCCAT CCACTGAAAA CCAAGAGGCC AAGAGAGTGG CCTTAGAGTG TATAAAGCAA
    TGTGACCCAG AAAAAATGAT CACAGAAAGC AAGTTCCTCC AGCTGGAGTC ACTACAGGAG
    CTCATGAAGG CTCTGGTCTC AGTGACACCA GATGAAGAGA CATATGATGA GGAAGATGCT
    GCTTTCTGCC TAGAGATGCT GCTAAGGATT GTGTTGGAGA ACAGGGATCG TGTGGGCTGT
    GTGTGGCAGA CTGTTCGAGA CCATCTATAC CACCTCTGTG TTCAGGCACA AGATTTCTGC
    TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG CTACGCCTGG CCATTCGGCT TCTCCGGAGA
    GAAGAGATCA GTGCTCAGGT GCTGCTCTCC CTGCGCATTT TGCTACTGAT GAAGCCCAGT
    GTGCTATCCC GAGTCAGCCA CCAGGTTGCG TATGGGCTCC ATGAACTCCT GAAGACCAAT
    GCAGCCAACA TCCACTCAGG TGATGACTGG GCCACACTCT TCACACTGCT GGAGTGCATC
    GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG CAGGCCACAG CCAGGGCAGA TGCACCTGAT
    GCCGGGGCCC AGTCAGATAG TGAGCTCCCA TCCTACCATC AGAATGACGT GAGCCTGGAT
    CGAGGGTACA CTTCCGACTC AGAGGTCTAC ACTGACCATG GCAGGCCGGG CAAGATACAC
    CGATCAGCCA CAGATGCCGA TGTGGTCAAC AGTGGTTGGT TAGTGGTTGG GAAGGATGAC
    GTTGATAACT CCAAGCCAGG GCCCAGCCGC CCAGGCCCTT CACCCCTGAT CAATCAATAC
    AGCCTAACAG TGGGACTGGA TTTGGGGCCA CACGACACTA AGTCTCTGCT TAAGTGTGTG
    GAATCGCTGT CCTTCATTGT GCGTGATGCT GCCCACATCA CACCTGACAA CTTTGAGCTC
    TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG GCCAGTCTGA ATGGCGGATG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGGA ACCGCTTCAA GAAGAAATCC
    AAAGAGGGAT CAATGCTTCG CCGGCCTCGA ACCTCCAGCC AACATGCCTC TCGGGGCGGG
    CAGAGTGATG ATGATGAGGA CGAAGGCGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGCTAGA CCTGATGCAC ACCCTGCACA CGCGGGCAGC CTCTATCTAC
    AGCTCATGGG CGGAGGAGCA ACGCCACCTG GAGACAGGTG GCCAGAAGAT TGAAGCTGAT
    TCTCGCACCC TCTGGGCCCA CTGCTGGTGC CCTTTACTGC AGGGTATTGC CTGCCTGTGC
    TGCGATGCCC GGCGCCAGGT ACGGATGCAG GCACTGACCT ATCTGCAGCG AGCACTACTT
    GTACATGATC TGCAAAAGCT AGATGCCCTG GAATGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TTACCAAGCT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACCCGGA TGAGGGCTTC CACATTGCTC TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTCACCA TCTTGGACTT CATGGACAAG
    TACATGCACG CAGGCTCCAG CGACTTACTG TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC
    ATGCTTCTGG TGATGGACAC AGCGGAGATT TTCCACAGTG CAGATGCACG GGGAGGCGGC
    CCCTCGGCCC TCTGGGAGAT CACCTGGGAA CGCATTGACT GTTTTCTCCC TCACCTACGA
    GATGAACTCT TCAAGCAGAC CGTCATCCAG GACCCCATGC CCATGGAGCC TCAAGGCCAA
    AAGCCTCTCG CCTCAGCCCA CCTGACTTCC GCTGCTGGCG ACACTAGGAC ACCTGGCCAT
    CCACCGCCCC CAGAGATTCC ATCTGAGCTG GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC
    CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA TCACCAGTGG CCTCAAGCCC CAGCAGGCTG
    AGCCCCACCC CCGACGGGCC TCCACCCTTG GCTCAGCCCC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAC TGCAGGTGGG CGTGCCACCT ATGACTCTGC CCATCATCCT CAACCCTGCG
    CTCATCGAGG CCACCTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGATCCCATA
    CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62152
    Clone ID Related Accession (Same CDS sequence) XM_011540313.1 , XM_011540313.2
    Accession Version XM_011540313.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538615.1
    CDS295..5211
    Translation

    Target ORF information:

    RefSeq Version XM_011540313.1
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62152
    Clone ID Related Accession (Same CDS sequence) XM_011540313.1 , XM_011540313.2
    Accession Version XM_011540313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538615.1
    CDS262..5178
    Translation

    Target ORF information:

    RefSeq Version XM_011540313.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC 
    AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG
    CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT
    TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA
    CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGA TATCTCCATG TCTCCTCAAC
    AAACTGGAGC TAAGTAGTGG GGAGCAGACC AAAGCCCTGA ACCAGTTAGA GAGGGTACTA
    CTCTTTAAGA ACCTCAAGCT GAAAATGAGA GCCGGAGGCA TGAGTGATTC ATCCAAATGG
    AAGAAACAGA AGAGATCCCC TCGGCCCCCA CGCCATATGA CCAAAGTCAC ACCAGGTTCA
    GAGCTGCCCA CTCCCAATGG AACCACCTTA TCATCTAACC TCACTGGTGG CATGCCCTTC
    ATTGATGTGC CCACTCCCAT CTCCTCTGCA AGTTCAGAAG CTGCCTCAGC AGTGGTCAGT
    CCCTCTACAG ACAGTGGCCT GGAATTCTCC TCCCAAACCA CTTCCAAGGA AGACCTTACT
    GATCTAGAGC AACCTGGCTC TCCAGGGTAC AGCACAGCTA CAGAGCCTGG AAGCAGTGAG
    CTAGGTGTTC CCGAGCAGCC TGACCTCCAG CAGGAAGGGA CCCATGTGGA AAAGTCCCAG
    TCAGCATCTG TGGAGTCCAT CCCTGAAGTG TTAGAGGAGT GCACGTCCCC TGCCGACCAC
    TCTGACTCTG CCTCTGTCCA TGACATGGAT TACGTCAATC CCCGGGGCGT GCGCTTTACA
    CAGTCCTCCC AGAAAGAAGG CACAGCTTTG GTCCCCTATG GTCTTCCCTG CATCCGCGAG
    CTCTTCCGCT TCCTCATCTC CCTCACCAAT CCACACGACC GCCATAACTC AGAGGTTATG
    ATTCACATGG GACTGCATTT GCTGACAGTG GCCCTTGAGT CAGCCCCTGT AGCCCAGTGC
    CAGACCCTCT TGGGCCTCAT CAAGGATGAG ATGTGCCGTC ACTTATTCCA GCTACTCAGC
    ATAGAGCGAC TAAACCTTTA TGCTGCTTCC CTGCGAGTAT GCTTCCTACT GTTTGAGAGC
    ATGCGAGAGC ACCTCAAGTT CCAAATGGAG ATGTACATCA AAAAGCTTAT GGAGATCATC
    ACTGTGGAGA ACCCCAAGAT GCCTTATGAG ATGAAGGAGA TGGCACTGGA GGCCATTGTG
    CAGCTCTGGC GCATCCCCAG CTTTGTCACA GAGCTCTACA TCAACTATGA TTGTGACTAC
    TACTGTTCCA ACCTCTTTGA GGAACTCACA AAGCTGCTGT CCAAGAATGC CTTCCCTGTG
    TCTGGTCAAC TCTATACAAC ACACCTACTA TCTCTTGATG CCCTATTGAC AGTGATTGAC
    AGCACCGAGG CCCACTGCCA GGCTAAAGTC CTCAACAGCC TCACCCAGCA AGAGAAGAAG
    GAGACAGCCA GACCAAGCTG TGAGATAGTA GATGGCACCC GAGAAGCTAG CAATACTGAG
    AGAACTGCCA GCGATGGGAA AGCTGTAGGC ATGGCCTCAG ACATCCCAGG CCTGCATCTG
    CCAGGTGGAG GGCGGCTGCC ACCAGAACAT GGGAAATCAG GATGCAGTGA TCTGGAGGAA
    GCTGTTGACT CTGGGGCTGA CAAAAAGTTT GCCCGGAAGC CACCCCGATT TTCCTGTCTC
    CTGCCAGATC CACGGGAACT AATTGAAATT AAAAACAAAA AGAAGCTGCT AATCACTGGC
    ACAGAGCAGT TCAATCAGAA ACCAAAGAAG GGGATTCAGT TTCTGCAAGA GAAAGGCCTC
    CTCACCATCC CAATGGACAA CACAGAGGTT GCTCAGTGGC TCCGAGAGAA CCCTCGGCTG
    GACAAGAAGA TGATTGGAGA GTTTGTGAGT GACCGCAAAA ACATTGACCT GTTGGAGAGC
    TTTGTGAGCA CCTTCAGTTT TCAGGGTCTG CGACTGGACG AAGCCCTCCG CCTCTACCTG
    GAAGCCTTCC GTTTGCCTGG GGAAGCACCA GTCATCCAGA GGTTGCTGGA GGCATTCACA
    GAGCGTTGGA TGAATTGTAA TGGCTCCCCA TTTGCCAATA GCGATGCCTG CTTTTCCCTG
    GCCTATGCTG TCATCATGCT TAATACTGAC CAGCACAACC ACAATGTTCG TAAACAGAAT
    GCACCCATGA CCCTGGAGGA GTTTCGCAAA AATCTGAAAG GTGTGAATGG AGGCAAGGAC
    TTTGAGCAAG ACATCCTGGA GGACATGTAC CATGCCATCA AGAATGAGGA AATTGTAATG
    CCTGAGGAGC AGACAGGCTT GGTTCGGGAG AACTATGTGT GGAATGTGCT GCTTCATCGA
    GGTGCCACCC CTGAGGGCAT ATTCCTGCGT GTGCCTACTG CCAGCTATGA TCTTGACCTC
    TTCACCATGA CCTGGGGCCC CACTATTGCT GCTCTCTCTT ATGTCTTTGA CAAAAGCCTT
    GAGGAGACAA TCATCCAGAA AGCCATCTCA GGCTTCAGGA AGTGCGCCAT GATCTCCGCC
    CACTATGGCC TCAGCGATGT GTTTGACAAT CTCATCATCT CTCTATGCAA ATTCACAGCT
    CTCAGCAGTG AGTCTATTGA GAACCTGCCC AGTGTATTTG GAAGCAACCC TAAAGCCCAT
    ATTGCAGCCA AGACAGTATT CCATTTGGCC CATCGTCATG GTGACATCCT GCGGGAGGGC
    TGGAAGAATA TCATGGAGGC CATGCTGCAG CTCTTCCGAG CCCAACTACT GCCCAAGGCT
    ATGATAGAGG TAGAAGATTT CGTGGATCCC AATGGCAAGA TCTCTCTACA GCGGGAAGAG
    ACACCATCAA ACCGAGGAGA GTCAACAGTG CTGAGCTTTG TGAGCTGGCT AACACTGAGT
    GGTCCTGAGC AGTCTAGTGT TCGGGGCCCA TCCACTGAAA ACCAAGAGGC CAAGAGAGTG
    GCCTTAGAGT GTATAAAGCA ATGTGACCCA GAAAAAATGA TCACAGAAAG CAAGTTCCTC
    CAGCTGGAGT CACTACAGGA GCTCATGAAG GCTCTGGTCT CAGTGACACC AGATGAAGAG
    ACATATGATG AGGAAGATGC TGCTTTCTGC CTAGAGATGC TGCTAAGGAT TGTGTTGGAG
    AACAGGGATC GTGTGGGCTG TGTGTGGCAG ACTGTTCGAG ACCATCTATA CCACCTCTGT
    GTTCAGGCAC AAGATTTCTG CTTCCTTGTG GAGCGGGCAG TGGTGGGGTT GCTACGCCTG
    GCCATTCGGC TTCTCCGGAG AGAAGAGATC AGTGCTCAGG TGCTGCTCTC CCTGCGCATT
    TTGCTACTGA TGAAGCCCAG TGTGCTATCC CGAGTCAGCC ACCAGGTTGC GTATGGGCTC
    CATGAACTCC TGAAGACCAA TGCAGCCAAC ATCCACTCAG GTGATGACTG GGCCACACTC
    TTCACACTGC TGGAGTGCAT CGGCTCAGGT GTGAAGCCTC CAGCTGCTCT GCAGGCCACA
    GCCAGGGCAG ATGCACCTGA TGCCGGGGCC CAGTCAGATA GTGAGCTCCC ATCCTACCAT
    CAGAATGACG TGAGCCTGGA TCGAGGGTAC ACTTCCGACT CAGAGGTCTA CACTGACCAT
    GGCAGGCCGG GCAAGATACA CCGATCAGCC ACAGATGCCG ATGTGGTCAA CAGTGGTTGG
    TTAGTGGTTG GGAAGGATGA CGTTGATAAC TCCAAGCCAG GGCCCAGCCG CCCAGGCCCT
    TCACCCCTGA TCAATCAATA CAGCCTAACA GTGGGACTGG ATTTGGGGCC ACACGACACT
    AAGTCTCTGC TTAAGTGTGT GGAATCGCTG TCCTTCATTG TGCGTGATGC TGCCCACATC
    ACACCTGACA ACTTTGAGCT CTGCGTCAAG ACTCTCCGGA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGAT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGG
    AACCGCTTCA AGAAGAAATC CAAAGAGGGA TCAATGCTTC GCCGGCCTCG AACCTCCAGC
    CAACATGCCT CTCGGGGCGG GCAGAGTGAT GATGATGAGG ACGAAGGCGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGCTAG ACCTGATGCA CACCCTGCAC
    ACGCGGGCAG CCTCTATCTA CAGCTCATGG GCGGAGGAGC AACGCCACCT GGAGACAGGT
    GGCCAGAAGA TTGAAGCTGA TTCTCGCACC CTCTGGGCCC ACTGCTGGTG CCCTTTACTG
    CAGGGTATTG CCTGCCTGTG CTGCGATGCC CGGCGCCAGG TACGGATGCA GGCACTGACC
    TATCTGCAGC GAGCACTACT TGTACATGAT CTGCAAAAGC TAGATGCCCT GGAATGGGAG
    TCCTGTTTTA ACAAGGTGGG ACTTCCTACT GGTCTTAAGA TAAAGTTCAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62153
    Clone ID Related Accession (Same CDS sequence) XM_011540314.2
    Accession Version XM_011540314.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACATGGA TTACGTCAAT CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG 
    CTACTCAGCA TAGAGCGACT AAACCTTTAT GCTGCTTCCC TGCGAGTATG CTTCCTACTG
    TTTGAGAGCA TGCGAGAGCA CCTCAAGTTC CAAATGGAGA TGTACATCAA AAAGCTTATG
    GAGATCATCA CTGTGGAGAA CCCCAAGATG CCTTATGAGA TGAAGGAGAT GGCACTGGAG
    GCCATTGTGC AGCTCTGGCG CATCCCCAGC TTTGTCACAG AGCTCTACAT CAACTATGAT
    TGTGACTACT ACTGTTCCAA CCTCTTTGAG GAACTCACAA AGCTGCTGTC CAAGAATGCC
    TTCCCTGTGT CTGGTCAACT CTATACAACA CACCTACTAT CTCTTGATGC CCTATTGACA
    GTGATTGACA GCACCGAGGC CCACTGCCAG GCTAAAGTCC TCAACAGCCT CACCCAGCAA
    GAGAAGAAGG AGACAGCCAG ACCAAGCTGT GAGATAGTAG ATGGCACCCG AGAAGCTAGC
    AATACTGAGA GAACTGCCAG CGATGGGAAA GCTGTAGGCA TGGCCTCAGA CATCCCAGGC
    CTGCATCTGC CAGGTGGAGG GCGGCTGCCA CCAGAACATG GGAAATCAGG ATGCAGTGAT
    CTGGAGGAAG CTGTTGACTC TGGGGCTGAC AAAAAGTTTG CCCGGAAGCC ACCCCGATTT
    TCCTGTCTCC TGCCAGATCC ACGGGAACTA ATTGAAATTA AAAACAAAAA GAAGCTGCTA
    ATCACTGGCA CAGAGCAGTT CAATCAGAAA CCAAAGAAGG GGATTCAGTT TCTGCAAGAG
    AAAGGCCTCC TCACCATCCC AATGGACAAC ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC
    CCTCGGCTGG ACAAGAAGAT GATTGGAGAG TTTGTGAGTG ACCGCAAAAA CATTGACCTG
    TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT CAGGGTCTGC GACTGGACGA AGCCCTCCGC
    CTCTACCTGG AAGCCTTCCG TTTGCCTGGG GAAGCACCAG TCATCCAGAG GTTGCTGGAG
    GCATTCACAG AGCGTTGGAT GAATTGTAAT GGCTCCCCAT TTGCCAATAG CGATGCCTGC
    TTTTCCCTGG CCTATGCTGT CATCATGCTT AATACTGACC AGCACAACCA CAATGTTCGT
    AAACAGAATG CACCCATGAC CCTGGAGGAG TTTCGCAAAA ATCTGAAAGG TGTGAATGGA
    GGCAAGGACT TTGAGCAAGA CATCCTGGAG GACATGTACC ATGCCATCAA GAATGAGGAA
    ATTGTAATGC CTGAGGAGCA GACAGGCTTG GTTCGGGAGA ACTATGTGTG GAATGTGCTG
    CTTCATCGAG GTGCCACCCC TGAGGGCATA TTCCTGCGTG TGCCTACTGC CAGCTATGAT
    CTTGACCTCT TCACCATGAC CTGGGGCCCC ACTATTGCTG CTCTCTCTTA TGTCTTTGAC
    AAAAGCCTTG AGGAGACAAT CATCCAGAAA GCCATCTCAG GCTTCAGGAA GTGCGCCATG
    ATCTCCGCCC ACTATGGCCT CAGCGATGTG TTTGACAATC TCATCATCTC TCTATGCAAA
    TTCACAGCTC TCAGCAGTGA GTCTATTGAG AACCTGCCCA GTGTATTTGG AAGCAACCCT
    AAAGCCCATA TTGCAGCCAA GACAGTATTC CATTTGGCCC ATCGTCATGG TGACATCCTG
    CGGGAGGGCT GGAAGAATAT CATGGAGGCC ATGCTGCAGC TCTTCCGAGC CCAACTACTG
    CCCAAGGCTA TGATAGAGGT AGAAGATTTC GTGGATCCCA ATGGCAAGAT CTCTCTACAG
    CGGGAAGAGA CACCATCAAA CCGAGGAGAG TCAACAGTGC TGAGCTTTGT GAGCTGGCTA
    ACACTGAGTG GTCCTGAGCA GTCTAGTGTT CGGGGCCCAT CCACTGAAAA CCAAGAGGCC
    AAGAGAGTGG CCTTAGAGTG TATAAAGCAA TGTGACCCAG AAAAAATGAT CACAGAAAGC
    AAGTTCCTCC AGCTGGAGTC ACTACAGGAG CTCATGAAGG CTCTGGTCTC AGTGACACCA
    GATGAAGAGA CATATGATGA GGAAGATGCT GCTTTCTGCC TAGAGATGCT GCTAAGGATT
    GTGTTGGAGA ACAGGGATCG TGTGGGCTGT GTGTGGCAGA CTGTTCGAGA CCATCTATAC
    CACCTCTGTG TTCAGGCACA AGATTTCTGC TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG
    CTACGCCTGG CCATTCGGCT TCTCCGGAGA GAAGAGATCA GTGCTCAGGT GCTGCTCTCC
    CTGCGCATTT TGCTACTGAT GAAGCCCAGT GTGCTATCCC GAGTCAGCCA CCAGGTTGCG
    TATGGGCTCC ATGAACTCCT GAAGACCAAT GCAGCCAACA TCCACTCAGG TGATGACTGG
    GCCACACTCT TCACACTGCT GGAGTGCATC GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG
    CAGGCCACAG CCAGGGCAGA TGCACCTGAT GCCGGGGCCC AGTCAGATAG TGAGCTCCCA
    TCCTACCATC AGAATGACGT GAGCCTGGAT CGAGGGTACA CTTCCGACTC AGAGGTCTAC
    ACTGACCATG GCAGGCCGGG CAAGATACAC CGATCAGCCA CAGATGCCGA TGTGGTCAAC
    AGTGGTTGGT TAGTGGTTGG GAAGGATGAC GTTGATAACT CCAAGCCAGG GCCCAGCCGC
    CCAGGCCCTT CACCCCTGAT CAATCAATAC AGCCTAACAG TGGGACTGGA TTTGGGGCCA
    CACGACACTA AGTCTCTGCT TAAGTGTGTG GAATCGCTGT CCTTCATTGT GCGTGATGCT
    GCCCACATCA CACCTGACAA CTTTGAGCTC TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG
    GCCAGTCTGA ATGGCGGATG CAAGTCCCAG GAGAAACGTG GCAAGAGTCA CAAATATGAC
    AGCAAAGGGA ACCGCTTCAA GAAGAAATCC AAAGAGGGAT CAATGCTTCG CCGGCCTCGA
    ACCTCCAGCC AACATGCCTC TCGGGGCGGG CAGAGTGATG ATGATGAGGA CGAAGGCGTG
    CCTGCCAGCT ACCATACGGT GTCTTTACAG GTCAGTCAGG ACTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538616.1
    CDS104..4354
    Translation

    Target ORF information:

    RefSeq Version XM_011540314.2
    Organism Homo sapiens(human)
    Definition Homo sapiens golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540314.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACATGGA TTACGTCAAT CCCCGGGGCG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG 
    CTACTCAGCA TAGAGCGACT AAACCTTTAT GCTGCTTCCC TGCGAGTATG CTTCCTACTG
    TTTGAGAGCA TGCGAGAGCA CCTCAAGTTC CAAATGGAGA TGTACATCAA AAAGCTTATG
    GAGATCATCA CTGTGGAGAA CCCCAAGATG CCTTATGAGA TGAAGGAGAT GGCACTGGAG
    GCCATTGTGC AGCTCTGGCG CATCCCCAGC TTTGTCACAG AGCTCTACAT CAACTATGAT
    TGTGACTACT ACTGTTCCAA CCTCTTTGAG GAACTCACAA AGCTGCTGTC CAAGAATGCC
    TTCCCTGTGT CTGGTCAACT CTATACAACA CACCTACTAT CTCTTGATGC CCTATTGACA
    GTGATTGACA GCACCGAGGC CCACTGCCAG GCTAAAGTCC TCAACAGCCT CACCCAGCAA
    GAGAAGAAGG AGACAGCCAG ACCAAGCTGT GAGATAGTAG ATGGCACCCG AGAAGCTAGC
    AATACTGAGA GAACTGCCAG CGATGGGAAA GCTGTAGGCA TGGCCTCAGA CATCCCAGGC
    CTGCATCTGC CAGGTGGAGG GCGGCTGCCA CCAGAACATG GGAAATCAGG ATGCAGTGAT
    CTGGAGGAAG CTGTTGACTC TGGGGCTGAC AAAAAGTTTG CCCGGAAGCC ACCCCGATTT
    TCCTGTCTCC TGCCAGATCC ACGGGAACTA ATTGAAATTA AAAACAAAAA GAAGCTGCTA
    ATCACTGGCA CAGAGCAGTT CAATCAGAAA CCAAAGAAGG GGATTCAGTT TCTGCAAGAG
    AAAGGCCTCC TCACCATCCC AATGGACAAC ACAGAGGTTG CTCAGTGGCT CCGAGAGAAC
    CCTCGGCTGG ACAAGAAGAT GATTGGAGAG TTTGTGAGTG ACCGCAAAAA CATTGACCTG
    TTGGAGAGCT TTGTGAGCAC CTTCAGTTTT CAGGGTCTGC GACTGGACGA AGCCCTCCGC
    CTCTACCTGG AAGCCTTCCG TTTGCCTGGG GAAGCACCAG TCATCCAGAG GTTGCTGGAG
    GCATTCACAG AGCGTTGGAT GAATTGTAAT GGCTCCCCAT TTGCCAATAG CGATGCCTGC
    TTTTCCCTGG CCTATGCTGT CATCATGCTT AATACTGACC AGCACAACCA CAATGTTCGT
    AAACAGAATG CACCCATGAC CCTGGAGGAG TTTCGCAAAA ATCTGAAAGG TGTGAATGGA
    GGCAAGGACT TTGAGCAAGA CATCCTGGAG GACATGTACC ATGCCATCAA GAATGAGGAA
    ATTGTAATGC CTGAGGAGCA GACAGGCTTG GTTCGGGAGA ACTATGTGTG GAATGTGCTG
    CTTCATCGAG GTGCCACCCC TGAGGGCATA TTCCTGCGTG TGCCTACTGC CAGCTATGAT
    CTTGACCTCT TCACCATGAC CTGGGGCCCC ACTATTGCTG CTCTCTCTTA TGTCTTTGAC
    AAAAGCCTTG AGGAGACAAT CATCCAGAAA GCCATCTCAG GCTTCAGGAA GTGCGCCATG
    ATCTCCGCCC ACTATGGCCT CAGCGATGTG TTTGACAATC TCATCATCTC TCTATGCAAA
    TTCACAGCTC TCAGCAGTGA GTCTATTGAG AACCTGCCCA GTGTATTTGG AAGCAACCCT
    AAAGCCCATA TTGCAGCCAA GACAGTATTC CATTTGGCCC ATCGTCATGG TGACATCCTG
    CGGGAGGGCT GGAAGAATAT CATGGAGGCC ATGCTGCAGC TCTTCCGAGC CCAACTACTG
    CCCAAGGCTA TGATAGAGGT AGAAGATTTC GTGGATCCCA ATGGCAAGAT CTCTCTACAG
    CGGGAAGAGA CACCATCAAA CCGAGGAGAG TCAACAGTGC TGAGCTTTGT GAGCTGGCTA
    ACACTGAGTG GTCCTGAGCA GTCTAGTGTT CGGGGCCCAT CCACTGAAAA CCAAGAGGCC
    AAGAGAGTGG CCTTAGAGTG TATAAAGCAA TGTGACCCAG AAAAAATGAT CACAGAAAGC
    AAGTTCCTCC AGCTGGAGTC ACTACAGGAG CTCATGAAGG CTCTGGTCTC AGTGACACCA
    GATGAAGAGA CATATGATGA GGAAGATGCT GCTTTCTGCC TAGAGATGCT GCTAAGGATT
    GTGTTGGAGA ACAGGGATCG TGTGGGCTGT GTGTGGCAGA CTGTTCGAGA CCATCTATAC
    CACCTCTGTG TTCAGGCACA AGATTTCTGC TTCCTTGTGG AGCGGGCAGT GGTGGGGTTG
    CTACGCCTGG CCATTCGGCT TCTCCGGAGA GAAGAGATCA GTGCTCAGGT GCTGCTCTCC
    CTGCGCATTT TGCTACTGAT GAAGCCCAGT GTGCTATCCC GAGTCAGCCA CCAGGTTGCG
    TATGGGCTCC ATGAACTCCT GAAGACCAAT GCAGCCAACA TCCACTCAGG TGATGACTGG
    GCCACACTCT TCACACTGCT GGAGTGCATC GGCTCAGGTG TGAAGCCTCC AGCTGCTCTG
    CAGGCCACAG CCAGGGCAGA TGCACCTGAT GCCGGGGCCC AGTCAGATAG TGAGCTCCCA
    TCCTACCATC AGAATGACGT GAGCCTGGAT CGAGGGTACA CTTCCGACTC AGAGGTCTAC
    ACTGACCATG GCAGGCCGGG CAAGATACAC CGATCAGCCA CAGATGCCGA TGTGGTCAAC
    AGTGGTTGGT TAGTGGTTGG GAAGGATGAC GTTGATAACT CCAAGCCAGG GCCCAGCCGC
    CCAGGCCCTT CACCCCTGAT CAATCAATAC AGCCTAACAG TGGGACTGGA TTTGGGGCCA
    CACGACACTA AGTCTCTGCT TAAGTGTGTG GAATCGCTGT CCTTCATTGT GCGTGATGCT
    GCCCACATCA CACCTGACAA CTTTGAGCTC TGCGTCAAGA CTCTCCGGAT CTTTGTGGAG
    GCCAGTCTGA ATGGCGGATG CAAGTCCCAG GAGAAACGTG GCAAGAGTCA CAAATATGAC
    AGCAAAGGGA ACCGCTTCAA GAAGAAATCC AAAGAGGGAT CAATGCTTCG CCGGCCTCGA
    ACCTCCAGCC AACATGCCTC TCGGGGCGGG CAGAGTGATG ATGATGAGGA CGAAGGCGTG
    CCTGCCAGCT ACCATACGGT GTCTTTACAG GTCAGTCAGG ACTTGCTAGA CCTGATGCAC
    ACCCTGCACA CGCGGGCAGC CTCTATCTAC AGCTCATGGG CGGAGGAGCA ACGCCACCTG
    GAGACAGGTG GCCAGAAGAT TGAAGCTGAT TCTCGCACCC TCTGGGCCCA CTGCTGGTGC
    CCTTTACTGC AGGGTATTGC CTGCCTGTGC TGCGATGCCC GGCGCCAGGT ACGGATGCAG
    GCACTGACCT ATCTGCAGCG AGCACTACTT GTACATGATC TGCAAAAGCT AGATGCCCTG
    GAATGGGAGT CCTGTTTTAA CAAGGTGCTG TTTCCTCTAC TTACCAAGCT CTTGGAGAAC
    ATCAGCCCTG CAGATGTGGG TGGGATGGAG GAGACCCGGA TGAGGGCTTC CACATTGCTC
    TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA CTGCTGTCAC TCTCTACCTT TGCGGCCCTC
    TGGCTCACCA TCTTGGACTT CATGGACAAG TACATGCACG CAGGCTCCAG CGACTTACTG
    TCAGAGGCGA TCCCTGAGTC TCTGAAGAAC ATGCTTCTGG TGATGGACAC AGCGGAGATT
    TTCCACAGTG CAGATGCACG GGGAGGCGGC CCCTCGGCCC TCTGGGAGAT CACCTGGGAA
    CGCATTGACT GTTTTCTCCC TCACCTACGA GATGAACTCT TCAAGCAGAC CGTCATCCAG
    GACCCCATGC CCATGGAGCC TCAAGGCCAA AAGCCTCTCG CCTCAGCCCA CCTGACTTCC
    GCTGCTGGCG ACACTAGGAC ACCTGGCCAT CCACCGCCCC CAGAGATTCC ATCTGAGCTG
    GGGGCCTGTG ACTTTGAGAA GCCCGAGAGC CCCCGAGCCG CCAGCAGCAG CTCCCCAGGA
    TCACCAGTGG CCTCAAGCCC CAGCAGGCTG AGCCCCACCC CCGACGGGCC TCCACCCTTG
    GCTCAGCCCC CACTGATCCT GCAGCCCTTG GCCTCCCCAC TGCAGGTGGG CGTGCCACCT
    ATGACTCTGC CCATCATCCT CAACCCTGCG CTCATCGAGG CCACCTCACC AGTGCCCCTC
    CTGGCCACAC CCCGCCCCAC AGATCCCATA CCCACCTCTG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.