VPS54 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS54
Entrez Gene ID	51542
Full Name	VPS54, GARP complex subunit
Synonyms	HCC8,PPP1R164,SLP-8p,VPS54L,WR,hVps54L
General protein information	Preferred Names VPS54, GARP complex subunit Names vacuolar protein sorting-associated protein 54 hepatocellular carcinoma protein 8 protein phosphatase 1, regulatory subunit 164 tumor antigen HOM-HCC-8 tumor antigen SLP-8p vacuolar protein sorting 54 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p15-p14
Summary	This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008].
Disorder MIM:	614633

mRNA and Protein(s)

mRNA	Protein	Name
NM_016516.2	NP_057600.2	vacuolar protein sorting-associated protein 54 isoform 1
XM_017004316.1	XP_016859805.1	vacuolar protein sorting-associated protein 54 isoform X1
NM_016516.3	NP_057600.2	vacuolar protein sorting-associated protein 54 isoform 1
XM_006712029.3	XP_006712092.1	vacuolar protein sorting-associated protein 54 isoform X1
NM_001005739.1	NP_001005739.1	vacuolar protein sorting-associated protein 54 isoform 2
NM_001005739.2	NP_001005739.1	vacuolar protein sorting-associated protein 54 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

The small G protein Arl5 contributes to endosome-to-Golgi traffic by aiding the recruitment of the GARP complex to the Golgi.
Rosa-Ferreira C, Christis C, Torres IL, Munro S
Biology open4(4)474-81(2015 Mar)

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Goldstein JI, Jarskog LF, Hilliard C, Alfirevic A, Duncan L, Fourches D, Huang H, Lek M, Neale BM, Ripke S, Shianna K, Szatkiewicz JP, Tropsha A, van den Oord EJ, Cascorbi I, Dettling M, Gazit E, Goff DC, Holden AL, Kelly DL, Malhotra AK, Nielsen J, Pirmohamed M, Rujescu D, Werge T, Levy DL, Josiassen RC, Kennedy JL, Lieberman JA, Daly MJ, Sullivan PF
Nature communications54757(2014 Sep)

VPS54 genetic analysis in ALS Italian cohort.
Corrado L, Gagliardi S, Carlomagno Y, Mennini T, Ticozzi N, Mazzini L, Silani V, Cereda C, D'Alfonso S
European journal of neurology18(4)e41-2(2011 Apr)

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
P?rez-Victoria FJ, Schindler C, Magad?n JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS
Molecular biology of the cell21(19)3386-95(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study suggests that VPS54 mutations are not a common cause of Amyotrophic Lateral Sclerosis in Italian population
Title: VPS54 genetic analysis in ALS Italian cohort.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Severely reduced levels of mutant Vps54 and, consequently, of the whole GARP complex underlie the phenotype of the wobbler mouse.
Title: Structural basis for the wobbler mouse neurodegenerative disorder caused by mutation in the Vps54 subunit of the GARP complex.

Golgi-associated retrograde protein orchestrates retrograde transport from endosomes to the trans-Golgi network by promoting vesicle tethering and assembly of SNARE complexes in consecutive, independent steps.[Golgi associated retrograde protein]
Title: Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.

Observational study of gene-disease association. (HuGE Navigator)
Title: Evaluation of the Golgi trafficking protein VPS54 (wobbler) as a candidate for ALS.

The following VPS54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28180	NM_016516.3 Latest version!	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu28195	NM_001005739.2 Latest version!	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu35432	XM_006712029.3 Latest version!	Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu89567	XM_017004316.1 Latest version!	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu28180	NM_016516.2	Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28195	NM_001005739.1	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS54 ( NM_016516.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057600.2 Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28180
Clone ID Related Accession (Same CDS sequence)	NM_016516.2 , NM_016516.3
Accession Version	NM_016516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092588.2, BC030275.1, AL514510.3, AF102177.1, BC041868.1, AK002205.1 and AW452246.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016516.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.194861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272322.9/ ENSP00000272322.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA 
AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC 
TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT 
TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA 
GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG 
GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA 
TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA 
GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA 
GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT 
CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT 
ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057600.2
CDS	512..3445
Translation	MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTGDSHSLYVAPSLVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_016516.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016516.3

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA 
AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC 
TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT 
TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA 
GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG 
GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA 
TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA 
GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA 
GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT 
CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT 
ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( NM_001005739.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005739.1 Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28195
Clone ID Related Accession (Same CDS sequence)	NM_001005739.1 , NM_001005739.2
Accession Version	NM_001005739.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030275.1, AL514510.3, BC041868.1, AK002205.1 and AW452246.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in one of the 5' coding exons compared to transcript variant 1, resulting in a shorter isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.54900.1, BC030275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC 
ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT 
GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT 
GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG 
GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT 
CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT 
GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT 
ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC 
ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC 
TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG 
AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT 
ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT 
CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA 
CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT 
TGGGAGCAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005739.1
CDS	164..3061
Translation	MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_001005739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005739.1

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC 
ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT 
GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT 
GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG 
GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT 
CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT 
GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT 
ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC 
ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC 
TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG 
AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT 
ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT 
CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA 
CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT 
TGGGAGCAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( NM_001005739.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005739.1 Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28195
Clone ID Related Accession (Same CDS sequence)	NM_001005739.1 , NM_001005739.2
Accession Version	NM_001005739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092588.2, BC030275.1, AL514510.3, BC041868.1, AK002205.1 and AW452246.1. On May 31, 2019 this sequence version replaced NM_001005739.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in one of the 5' coding exons compared to transcript variant 1, resulting in a shorter isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.54900.1, BC030275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC 
ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT 
GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT 
GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG 
GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT 
CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT 
GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT 
ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC 
ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC 
TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG 
AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT 
ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT 
CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA 
CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT 
TGGGAGCAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005739.1
CDS	512..3409
Translation	MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_001005739.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005739.2

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC 
ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT 
GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT 
GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG 
GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT 
CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT 
GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT 
ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC 
ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC 
TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG 
AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT 
ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT 
CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA 
CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT 
TGGGAGCAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( NM_016516.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057600.2 Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28180
Clone ID Related Accession (Same CDS sequence)	NM_016516.2 , NM_016516.3
Accession Version	NM_016516.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030275.1, AL514510.3, AF102177.1, BC041868.1, AK002205.1 and AW452246.1. This sequence is a reference standard in the RefSeqGene project. On Oct 15, 2004 this sequence version replaced NM_016516.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.194861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA 
AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC 
TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT 
TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA 
GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG 
GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA 
TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA 
GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA 
GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT 
CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT 
ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057600.2
CDS	164..3097
Translation	MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTGDSHSLYVAPSLVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_016516.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016516.2

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA 
AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC 
TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT 
TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA 
GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG 
GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA 
TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA 
GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA 
GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT 
CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT 
ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_006712029.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712092.1 Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35432
Clone ID Related Accession (Same CDS sequence)	XM_006712029.3
Accession Version	XM_006712029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGTGT 
GTGATTAATA AAGTTTCACA AACAGAAGAA ATAGACACAG ATGTTGTTGT GAAGCTTGCA 
GATCAGATGA GAATGTTGAA TTTTCCCCAG TGGTTTGATC TGCTCAAGGA TATTTTCTCT 
AAGTTTACAA TTTTCCTACA GAGAGTGAAG GCAACATTAA ATATCATTCA CAGTGTTGTT 
CTCTCAGTTC TTGACAAAAA CCAAAGGACT AGAGAATTGG AAGAGATTTC ACAACAGAAG 
AATGCTGCAA AAGATAATTC ACTGGACACA GAGGTGGCTT ATTTAATCCA TGAAGGCATG 
TTTATAAGTG ATGCATTCGG TGAGGGTGAG CTAACACCTA TAGCAGTTGA CACTACCTCT 
CAAAGAAATG CATCTCCAAA TAGTGAGCCC TGCAGCAGTG ATTCTGTATC CGAGCCAGAA 
TGTACTACTG ATTCTTCATC CAGCAAAGAG CACACATCAT CATCTGCTAT TCCAGGAGGT 
GTGGATATTA TGGTCAGTGA AGATATGAAA TTAACTGACT CAGAGCTAGG AAAGCTGGCA 
AATAATATCC AGGAATTATT ATATAGTGCC TCAGATATAT GCCATGATCG AGCTGTCAAA 
TTTCTCATGT CAAGAGCAAA GGATGGTTTT CTTGAGAAGC TAAATTCCAT GGAATTCATA 
ACACTTTCTA GATTAATGGA AACATTCATT TTAGACACCG AACAGATCTG TGGAAGAAAA 
AGCACGTCAT TACTTGGAGC ACTTCAGAGC CAAGCTATTA AGTTTGTAAA TAGGTTTCAT 
GAAGAGAGAA AAACCAAGCT CAGCCTCCTC TTAGACAATG AGCGCTGGAA GCAAGCAGAT 
GTTCCTGCAG AATTTCAGGA TCTTGTTGAT TCTCTGTCAG ATGGGAAGAT TGCTTTACCT 
GAAAAAAAAT CAGGAGCCAC AGAAGAAAGG AAACCAGCTG AAGTTCTTAT TGTCGAGGGA 
CAACAGTATG CAGTTGTTGG AACCGTATTG CTGTTAATAA GAATTATCCT TGAATATTGC 
CAGTGTGTGG ATAACATCCC ATCTGTTACT ACTGACATGC TTACTCGTCT GTCAGATTTA 
TTGAAGTACT TCAATTCAAG AAGTTGCCAG TTAGTTCTTG GAGCTGGTGC ACTGCAAGTT 
GTTGGACTAA AAACGATAAC TACAAAAAAT TTGGCTCTTT CTTCACGATG TTTGCAGTTA 
ATTGTGCACT ACATTCCTGT GATCCGGGCT CATTTTGAAG CTCGACTACC ACCTAAGCAA 
TATAGCATGC TTAGGCATTT TGATCATATC ACTAAGGACT ACCATGATCA CATAGCTGAA 
ATATCAGCTA AGCTTGTAGC GATAATGGAT AGCTTATTTG ACAAGCTGTT ATCTAAGTAT 
GAAGTGAAGG CTCCTGTTCC TTCTGCCTGT TTCAGGAATA TTTGTAAGCA AATGACAAAA 
ATGCACGAAG CTATATTTGA TCTCCTTCCA GAAGAACAAA CACAGATGTT ATTTTTAAGA 
ATTAATGCAA GTTATAAACT CCACTTGAAA AAGCAGTTAT CTCACTTAAA TGTGATAAAT 
GATGGAGGAC CTCAAAATGG GTTGGTCACA GCAGATGTAG CTTTTTACAC TGGAAATCTT 
CAAGCCTTAA AAGGCCTTAA AGATTTGGAC CTAAATATGG CCGAAATTTG GGAGCAGAAG 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712092.1
CDS	132..2957
Translation	MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTGDSHSLYVAPSLVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEECVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_006712029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712029.3

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT 
CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG 
TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGTGT 
GTGATTAATA AAGTTTCACA AACAGAAGAA ATAGACACAG ATGTTGTTGT GAAGCTTGCA 
GATCAGATGA GAATGTTGAA TTTTCCCCAG TGGTTTGATC TGCTCAAGGA TATTTTCTCT 
AAGTTTACAA TTTTCCTACA GAGAGTGAAG GCAACATTAA ATATCATTCA CAGTGTTGTT 
CTCTCAGTTC TTGACAAAAA CCAAAGGACT AGAGAATTGG AAGAGATTTC ACAACAGAAG 
AATGCTGCAA AAGATAATTC ACTGGACACA GAGGTGGCTT ATTTAATCCA TGAAGGCATG 
TTTATAAGTG ATGCATTCGG TGAGGGTGAG CTAACACCTA TAGCAGTTGA CACTACCTCT 
CAAAGAAATG CATCTCCAAA TAGTGAGCCC TGCAGCAGTG ATTCTGTATC CGAGCCAGAA 
TGTACTACTG ATTCTTCATC CAGCAAAGAG CACACATCAT CATCTGCTAT TCCAGGAGGT 
GTGGATATTA TGGTCAGTGA AGATATGAAA TTAACTGACT CAGAGCTAGG AAAGCTGGCA 
AATAATATCC AGGAATTATT ATATAGTGCC TCAGATATAT GCCATGATCG AGCTGTCAAA 
TTTCTCATGT CAAGAGCAAA GGATGGTTTT CTTGAGAAGC TAAATTCCAT GGAATTCATA 
ACACTTTCTA GATTAATGGA AACATTCATT TTAGACACCG AACAGATCTG TGGAAGAAAA 
AGCACGTCAT TACTTGGAGC ACTTCAGAGC CAAGCTATTA AGTTTGTAAA TAGGTTTCAT 
GAAGAGAGAA AAACCAAGCT CAGCCTCCTC TTAGACAATG AGCGCTGGAA GCAAGCAGAT 
GTTCCTGCAG AATTTCAGGA TCTTGTTGAT TCTCTGTCAG ATGGGAAGAT TGCTTTACCT 
GAAAAAAAAT CAGGAGCCAC AGAAGAAAGG AAACCAGCTG AAGTTCTTAT TGTCGAGGGA 
CAACAGTATG CAGTTGTTGG AACCGTATTG CTGTTAATAA GAATTATCCT TGAATATTGC 
CAGTGTGTGG ATAACATCCC ATCTGTTACT ACTGACATGC TTACTCGTCT GTCAGATTTA 
TTGAAGTACT TCAATTCAAG AAGTTGCCAG TTAGTTCTTG GAGCTGGTGC ACTGCAAGTT 
GTTGGACTAA AAACGATAAC TACAAAAAAT TTGGCTCTTT CTTCACGATG TTTGCAGTTA 
ATTGTGCACT ACATTCCTGT GATCCGGGCT CATTTTGAAG CTCGACTACC ACCTAAGCAA 
TATAGCATGC TTAGGCATTT TGATCATATC ACTAAGGACT ACCATGATCA CATAGCTGAA 
ATATCAGCTA AGCTTGTAGC GATAATGGAT AGCTTATTTG ACAAGCTGTT ATCTAAGTAT 
GAAGTGAAGG CTCCTGTTCC TTCTGCCTGT TTCAGGAATA TTTGTAAGCA AATGACAAAA 
ATGCACGAAG CTATATTTGA TCTCCTTCCA GAAGAACAAA CACAGATGTT ATTTTTAAGA 
ATTAATGCAA GTTATAAACT CCACTTGAAA AAGCAGTTAT CTCACTTAAA TGTGATAAAT 
GATGGAGGAC CTCAAAATGG GTTGGTCACA GCAGATGTAG CTTTTTACAC TGGAAATCTT 
CAAGCCTTAA AAGGCCTTAA AGATTTGGAC CTAAATATGG CCGAAATTTG GGAGCAGAAG 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_017004316.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859805.1 Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89567
Clone ID Related Accession (Same CDS sequence)	XM_017004316.1
Accession Version	XM_017004316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCC CAAGGAACCC ACAGAGAGAG AAGATTCATG AGAGATGCAA GAATATTTGT 
CCTCCTAAAG ATACCTTCGA AAGGACTCTT TTACATACTC ATGATAAATC CAGGACAGAT 
CTGGAGCAAG TACCTAAGAT TTTTATGAAA CCAGATTTTG CCTTGGATGA TTCCTTAACT 
TTTAATTCAG TTTTACCATG GTCTCATTTT AATACTGCTG GTGGAAAAGG AAATCGTGAT 
GCAGCTTCCT CAAAGTTGCT TCAAGAAAAG CTGAGCCATT ATCTGGATAT TGTGGAAGTA 
AACATTGCTC ACCAGATCTC TCTACGTTCA GAAGCATTTT TTCATGCAAT GACCTCTCAA 
CACGAGTTGC AGGACTACCT CAGGAAAACT TCCCAGGCTG TAAAAATGCT TCGAGATAAA 
ATTGCACAGA TTGATAAAGT AATGTGTGAA GGATCACTCC ACATTTTAAG ACTGGCACTT 
ACCAGAAATA ATTGTGTTAA AGTATACAAT AAGCTGAAGT TAATGGCCAC TGTACACCAG 
ACTCAGCCTA CAGTACAGGT GTTATTATCT ACTTCTGAAT TTGTTGGAGC ATTGGACTTA 
ATAGCAACAA CACAAGAGGT TCTACAGCAG GAACTTCAGG GCATTCACAG TTTCCGGCAT 
TTGGGATCAC AGCTTTGTGA ATTAGAAAAA CTGATAGATA AAATGATGAT TGCAGAATTT 
TCTACTTATT CTCACAGTGA CTTAAATAGA CCACTGGAAG ATGACTGTCA AGTTTTAGAA 
GAGGAAAGAC TAATATCTCT TGTATTTGGA CTTTTAAAAC AAAGAAAGCT TAATTTTTTA 
GAAATCTATG GTGAAAAAAT GGTTATTACA GCAAAGAATA TCATTAAACA GTGTGTGATT 
AATAAAGTTT CACAAACAGA AGAAATAGAC ACAGATGTTG TTGTGAAGCT TGCAGATCAG 
ATGAGAATGT TGAATTTTCC CCAGTGGTTT GATCTGCTCA AGGATATTTT CTCTAAGTTT 
ACAATTTTCC TACAGAGAGT GAAGGCAACA TTAAATATCA TTCACAGTGT TGTTCTCTCA 
GTTCTTGACA AAAACCAAAG GACTAGAGAA TTGGAAGAGA TTTCACAACA GAAGAATGCT 
GCAAAAGATA ATTCACTGGA CACAGAGGTG GCTTATTTAA TCCATGAAGG CATGTTTATA 
AGTGATGCAT TCGGTGAGGG TGAGCTAACA CCTATAGCAG TTGACACTAC CTCTCAAAGA 
AATGCATCTC CAAATAGTGA GCCCTGCAGC AGTGATTCTG TATCCGAGCC AGAATGTACT 
ACTGATTCTT CATCCAGCAA AGAGCACACA TCATCATCTG CTATTCCAGG AGGTGTGGAT 
ATTATGGTCA GTGAAGATAT GAAATTAACT GACTCAGAGC TAGGAAAGCT GGCAAATAAT 
ATCCAGGAAT TATTATATAG TGCCTCAGAT ATATGCCATG ATCGAGCTGT CAAATTTCTC 
ATGTCAAGAG CAAAGGATGG TTTTCTTGAG AAGCTAAATT CCATGGAATT CATAACACTT 
TCTAGATTAA TGGAAACATT CATTTTAGAC ACCGAACAGA TCTGTGGAAG AAAAAGCACG 
TCATTACTTG GAGCACTTCA GAGCCAAGCT ATTAAGTTTG TAAATAGGTT TCATGAAGAG 
AGAAAAACCA AGCTCAGCCT CCTCTTAGAC AATGAGCGCT GGAAGCAAGC AGATGTTCCT 
GCAGAATTTC AGGATCTTGT TGATTCTCTG TCAGATGGGA AGATTGCTTT ACCTGAAAAA 
AAATCAGGAG CCACAGAAGA AAGGAAACCA GCTGAAGTTC TTATTGTCGA GGGACAACAG 
TATGCAGTTG TTGGAACCGT ATTGCTGTTA ATAAGAATTA TCCTTGAATA TTGCCAGTGT 
GTGGATAACA TCCCATCTGT TACTACTGAC ATGCTTACTC GTCTGTCAGA TTTATTGAAG 
TACTTCAATT CAAGAAGTTG CCAGTTAGTT CTTGGAGCTG GTGCACTGCA AGTTGTTGGA 
CTAAAAACGA TAACTACAAA AAATTTGGCT CTTTCTTCAC GATGTTTGCA GTTAATTGTG 
CACTACATTC CTGTGATCCG GGCTCATTTT GAAGCTCGAC TACCACCTAA GCAATATAGC 
ATGCTTAGGC ATTTTGATCA TATCACTAAG GACTACCATG ATCACATAGC TGAAATATCA 
GCTAAGCTTG TAGCGATAAT GGATAGCTTA TTTGACAAGC TGTTATCTAA GTATGAAGTG 
AAGGCTCCTG TTCCTTCTGC CTGTTTCAGG AATATTTGTA AGCAAATGAC AAAAATGCAC 
GAAGCTATAT TTGATCTCCT TCCAGAAGAA CAAACACAGA TGTTATTTTT AAGAATTAAT 
GCAAGTTATA AACTCCACTT GAAAAAGCAG TTATCTCACT TAAATGTGAT AAATGATGGA 
GGACCTCAAA ATGGGTTGGT CACAGCAGAT GTAGCTTTTT ACACTGGAAA TCTTCAAGCC 
TTAAAAGGCC TTAAAGATTT GGACCTAAAT ATGGCCGAAA TTTGGGAGCA GAAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859805.1
CDS	198..2777
Translation	MCVPRNPQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_017004316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004316.1

ORF Insert Sequence:

ATGTGTGTCC CAAGGAACCC ACAGAGAGAG AAGATTCATG AGAGATGCAA GAATATTTGT 
CCTCCTAAAG ATACCTTCGA AAGGACTCTT TTACATACTC ATGATAAATC CAGGACAGAT 
CTGGAGCAAG TACCTAAGAT TTTTATGAAA CCAGATTTTG CCTTGGATGA TTCCTTAACT 
TTTAATTCAG TTTTACCATG GTCTCATTTT AATACTGCTG GTGGAAAAGG AAATCGTGAT 
GCAGCTTCCT CAAAGTTGCT TCAAGAAAAG CTGAGCCATT ATCTGGATAT TGTGGAAGTA 
AACATTGCTC ACCAGATCTC TCTACGTTCA GAAGCATTTT TTCATGCAAT GACCTCTCAA 
CACGAGTTGC AGGACTACCT CAGGAAAACT TCCCAGGCTG TAAAAATGCT TCGAGATAAA 
ATTGCACAGA TTGATAAAGT AATGTGTGAA GGATCACTCC ACATTTTAAG ACTGGCACTT 
ACCAGAAATA ATTGTGTTAA AGTATACAAT AAGCTGAAGT TAATGGCCAC TGTACACCAG 
ACTCAGCCTA CAGTACAGGT GTTATTATCT ACTTCTGAAT TTGTTGGAGC ATTGGACTTA 
ATAGCAACAA CACAAGAGGT TCTACAGCAG GAACTTCAGG GCATTCACAG TTTCCGGCAT 
TTGGGATCAC AGCTTTGTGA ATTAGAAAAA CTGATAGATA AAATGATGAT TGCAGAATTT 
TCTACTTATT CTCACAGTGA CTTAAATAGA CCACTGGAAG ATGACTGTCA AGTTTTAGAA 
GAGGAAAGAC TAATATCTCT TGTATTTGGA CTTTTAAAAC AAAGAAAGCT TAATTTTTTA 
GAAATCTATG GTGAAAAAAT GGTTATTACA GCAAAGAATA TCATTAAACA GTGTGTGATT 
AATAAAGTTT CACAAACAGA AGAAATAGAC ACAGATGTTG TTGTGAAGCT TGCAGATCAG 
ATGAGAATGT TGAATTTTCC CCAGTGGTTT GATCTGCTCA AGGATATTTT CTCTAAGTTT 
ACAATTTTCC TACAGAGAGT GAAGGCAACA TTAAATATCA TTCACAGTGT TGTTCTCTCA 
GTTCTTGACA AAAACCAAAG GACTAGAGAA TTGGAAGAGA TTTCACAACA GAAGAATGCT 
GCAAAAGATA ATTCACTGGA CACAGAGGTG GCTTATTTAA TCCATGAAGG CATGTTTATA 
AGTGATGCAT TCGGTGAGGG TGAGCTAACA CCTATAGCAG TTGACACTAC CTCTCAAAGA 
AATGCATCTC CAAATAGTGA GCCCTGCAGC AGTGATTCTG TATCCGAGCC AGAATGTACT 
ACTGATTCTT CATCCAGCAA AGAGCACACA TCATCATCTG CTATTCCAGG AGGTGTGGAT 
ATTATGGTCA GTGAAGATAT GAAATTAACT GACTCAGAGC TAGGAAAGCT GGCAAATAAT 
ATCCAGGAAT TATTATATAG TGCCTCAGAT ATATGCCATG ATCGAGCTGT CAAATTTCTC 
ATGTCAAGAG CAAAGGATGG TTTTCTTGAG AAGCTAAATT CCATGGAATT CATAACACTT 
TCTAGATTAA TGGAAACATT CATTTTAGAC ACCGAACAGA TCTGTGGAAG AAAAAGCACG 
TCATTACTTG GAGCACTTCA GAGCCAAGCT ATTAAGTTTG TAAATAGGTT TCATGAAGAG 
AGAAAAACCA AGCTCAGCCT CCTCTTAGAC AATGAGCGCT GGAAGCAAGC AGATGTTCCT 
GCAGAATTTC AGGATCTTGT TGATTCTCTG TCAGATGGGA AGATTGCTTT ACCTGAAAAA 
AAATCAGGAG CCACAGAAGA AAGGAAACCA GCTGAAGTTC TTATTGTCGA GGGACAACAG 
TATGCAGTTG TTGGAACCGT ATTGCTGTTA ATAAGAATTA TCCTTGAATA TTGCCAGTGT 
GTGGATAACA TCCCATCTGT TACTACTGAC ATGCTTACTC GTCTGTCAGA TTTATTGAAG 
TACTTCAATT CAAGAAGTTG CCAGTTAGTT CTTGGAGCTG GTGCACTGCA AGTTGTTGGA 
CTAAAAACGA TAACTACAAA AAATTTGGCT CTTTCTTCAC GATGTTTGCA GTTAATTGTG 
CACTACATTC CTGTGATCCG GGCTCATTTT GAAGCTCGAC TACCACCTAA GCAATATAGC 
ATGCTTAGGC ATTTTGATCA TATCACTAAG GACTACCATG ATCACATAGC TGAAATATCA 
GCTAAGCTTG TAGCGATAAT GGATAGCTTA TTTGACAAGC TGTTATCTAA GTATGAAGTG 
AAGGCTCCTG TTCCTTCTGC CTGTTTCAGG AATATTTGTA AGCAAATGAC AAAAATGCAC 
GAAGCTATAT TTGATCTCCT TCCAGAAGAA CAAACACAGA TGTTATTTTT AAGAATTAAT 
GCAAGTTATA AACTCCACTT GAAAAAGCAG TTATCTCACT TAAATGTGAT AAATGATGGA 
GGACCTCAAA ATGGGTTGGT CACAGCAGAT GTAGCTTTTT ACACTGGAAA TCTTCAAGCC 
TTAAAAGGCC TTAAAGATTT GGACCTAAAT ATGGCCGAAA TTTGGGAGCA GAAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

The small G protein Arl5 contributes to endosome-to-Golgi traffic by aiding the recruitment of the GARP complex to the Golgi.
Biology open4(4)474-81(2015 Mar)
Rosa-Ferreira C,Christis C,Torres IL,Munro S

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Nature communications54757(2014 Sep)
Goldstein JI,Jarskog LF,Hilliard C,Alfirevic A,Duncan L,Fourches D,Huang H,Lek M,Neale BM,Ripke S,Shianna K,Szatkiewicz JP,Tropsha A,van den Oord EJ,Cascorbi I,Dettling M,Gazit E,Goff DC,Holden AL,Kelly DL,Malhotra AK,Nielsen J,Pirmohamed M,Rujescu D,Werge T,Levy DL,Josiassen RC,Kennedy JL,Lieberman JA,Daly MJ,Sullivan PF

VPS54 genetic analysis in ALS Italian cohort.
European journal of neurology18(4)e41-2(2011 Apr)
Corrado L,Gagliardi S,Carlomagno Y,Mennini T,Ticozzi N,Mazzini L,Silani V,Cereda C,D'Alfonso S

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
Molecular biology of the cell21(19)3386-95(2010 Oct)
P?rez-Victoria FJ,Schindler C,Magad?n JG,Mardones GA,Delevoye C,Romao M,Raposo G,Bonifacino JS

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VPS54 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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