vps54 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps54
Entrez Gene ID	562169
Full Name	vacuolar protein sorting 54 homolog (S. cerevisiae)
Synonyms	zgc:195037
General protein information	Preferred Names vacuolar protein sorting 54 homolog (S. cerevisiae) Names vacuolar protein sorting-associated protein 54
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475329.1	XP_021331004.1	vacuolar protein sorting-associated protein 54 isoform X1
NM_001130776.1	NP_001124248.1	vacuolar protein sorting-associated protein 54

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Zhou S, Zhao H, Thongda W, Zhang D, Su B, Yu D, Peatman E, Li C
Fish & shellfish immunology49324-35(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vps54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33519	NM_001130776.1 Latest version!	Danio rerio VPS54 subunit of GARP complex (vps54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa147267	XM_021475329.1 Latest version!	Danio rerio vacuolar protein sorting 54 homolog (S. cerevisiae) (vps54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps54 ( NM_001130776.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124248.1 Danio rerio VPS54 subunit of GARP complex (vps54), mRNA.

Price & Availability↑

CloneID	ODa33519
Clone ID Related Accession (Same CDS sequence)	NM_001130776.1
Accession Version	NM_001130776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163517.1. On Aug 6, 2008 this sequence version replaced XM_685566.3. ##Evidence-Data-START## Transcript exon combination :: BC163517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCCA ATCAGGGCTC GTCTCCGATC CCTCAGTGCA GTGGGGCAGG AGGAGGAACC 
AGTGATGGGT TTTTCCGGAA GCACAGAGAG CCTCCACTGG CCCCCAAACA CTATCGGACG 
GCCCGCTCGC TGCCGGACGT CTGCCCTAAA GAGCCCACCG GTGATGGCGG TGGTCTGTGC 
GAGGGTCCGT CTGTTGTGGC CGATCAGCAC CGCTGGACTG TGTATAACTC TAAAGTGAAT 
CTCCCCGCGG CACTCAATGA CCCCCGGCTG GCCAAAAGAG AGTCTGACTT CTTCACTAAA 
ACCTGGGGCT TGGATTTCAG CGAGACCGAA GTCATGCCTT CATTCTACCT GCCCAACATC 
ACCCGAGAAC ACTTCAGCTC ATACCTGCAG GACACGGCTC AGAGGGAGAA GATTCACGAG 
CGCTGCAAGA ACATTTGCCC AAGCAAAGAC GATCTGTCAG TGTCCAGCAT CACTAATAAT 
CACGAAAAAG CCCGAGCTGA ACTGGAGCAG GTGCCCAAGA TCTTCATGAA GCCGGAGTTT 
GTGTTAAATG ATCCCGCCAC CTTTAATGCC GTGTTGCCGT GGTCTCACTT CAGCGTCGCC 
GGGGGAAAGA ATAGCAGAGA TGTTGCATCA TCACGGTTAC TACAGGAAAA GTTGAGTCAT 
TATCTGGACG TGGTGGAGGT GAGCATCGCT CGTCAGATCT CTCTTCGCTC CGAGGCCTTT 
TTCCACGCCA TGTCGTCTCA GCACGAGCTG CAGGACCAGC TGCGCGAGAC AGCGGACGCC 
GTGGCTAAAC TCCGCGCCCG AACCGCTGCC ATCGACCGAG TGATGTGCCG TGGGCCACTG 
CGAGTGCTAC GCGATGCTCT CACGCGCAAC AACTGCATCA AACTGCACAA CAAGCTGAAG 
CTGATGGCGG CCGTGCACCA GACGCAGCCC ACCGTACAGC TGCTGCTCTC CACCTCAGAG 
TTCGTGGGGG CGCTAGAGCT CATCGCCACC ACCAAAGAAG TGCTGCAGCA GGAACTGCAG 
GGCATTCACA GCTTCAGGCA TCTGGGTTCT CAGCTGTGTG AGATGGAGAG GCTGATCGAT 
AAGATGATGG TGGCGGATTT CTCCACTTAT GCTCAGAGTG ACCTCAGCCG AACCTTTGAG 
GAGGACGGTC AGGTTCTGGA GAAGGATCGC TTGCAGTCTC TAGTCTTTGG GCTGTTGCGG 
CAGAGGAAGC TGGATTTCCT GGACATCTAC AGTGAAGAGA TGATCCGCGC AGCTAAAAAC 
GTGGTCCGGC AGTGTGTTGT GAAGTCCCTG TCTCAGGTCA ATGAGGTCGA CGCAGACACT 
GTCAGGATTC ATGAACAGAT GCGCTTGATG GCGTTTCATC ATTGGTTTGA TCTGCTGCTG 
GATATATTTG AACACTTCAT TCTGTTCCTC AAGAGGATCA AGGCGACGCT CGGTGTCATC 
CGTAATGTTG TTCTGGAAGT GCTGGACAGC AGCCAGAGGA GTCGACTAGC GGATGCCACC 
ACAGCAACCA GTCATCCAGA TGGCAGAGTA AATGAGGATG AGGAGGAGGA GTCAGGAGGC 
CCCGCCCTTC ATCCAGGAGA GGCGGAGCTT GCGTACCTCA CTCACGAGGG TTTGTTCATC 
AGTGACGCTC TGAATGATGC AGAGCAGCGC AGTGTTACAA CAGCAGCACA AACACAGACC 
AGAGCACAGA CGCCGCGCGC AGACGCCTGC GGGAGCGACT CGGCCTCCGC AACCACTGAA 
TCCTCCTCCA GCAGGGAGCA CAATTCCAAC ACTACCTCCT GCCTGCCTGT AGGGGCCGCT 
GCGCTTGAGG ACGTCATGCC GTCTGACCTG GAGTTGGGTC GAGTGGCGAA TAACGTTCAG 
GAGCTGCTGT ATGCAGCGTC AGACGTCAGT CACGACCGCT GTGTCAAAGT GCTGATGACC 
AGAGCCAAGG ACGGCTCTCT AGAGCGCCTG AGCTCGTCAG AGTTTGTCTG TTTGAGTCAG 
GCCGTGGAGT CGTTTGTCAA AGACACTGAA GAGCTGTGCG GCAGGCGCAG TGTCAGCCTG 
AGGGGGGCGC TGCAGAGTCA GGCCAACCGC TTCGTACAGA GATTCCACGA GGAGCGCAAG 
ACCAAACTCA GTCTGCTGCT GGATAATGAG CGCTGGAAAC AGGCTGAGGT TCCTGCTGAG 
TTTCAGGATC TGGTCGACTC CATCGCTGAC GGACGAATCT CTCTGCCTGA GCGCAAACAC 
AGCAGCGGGT CCGAGGACAG GAAGCCCACT GAGTTTCTGT GTGTGGACGG ACAGAAGTAT 
GCGGTGGTCG GGACGGTACT GCTGCTGATC CGCATGTTTC TGGAGTACTG CCAGTGTGTG 
AACGACATTC CCTCCATCAC CACTGACATC ATCACACGCC TCGCCGACCT GCTCAAGCAC 
TTCAACTCTC GCTGTTGTCA GCTGGTTCTC GGTGCTGGAG CTCTGCAGGC TGTGGGACTC 
AAAACCATAA CCACCAGAAA CCTGGCTCTG GCGTCTCGCT GTCTTCAGCT GGTGGTTCAC 
TACATCCCTG TGATCAGAGC ACACTTTGAG ACGCGCCTGC AGCCCAAACA GTACAGCATA 
CTGAGACACT TTGACCACAT CACCAAGGAC TATAATGACC ACATCGCAGA GGTCTCGGCT 
AAGCTGGTGG CCATCATGGA CAGTATGTTC GAGAAGGCTG TGTCTAAGTA TGAGGTGAAG 
GCGCCGATGC CGTCTGCGTG CTTGCGTAAC GTGTGTAAGC AGATGCTGAA GATGCACGAG 
GCCATTCATG AGCTGCTGCC GGAGGAACAA ACGCAGATGC TGTTCCTCAG GATAAACGCC 
AGCTTTAAGC TCCACCTGAA GAGACAGTTG TCACGGCTGG GGGTCAGCAA TGATGGAGGA 
CCACAGAATG GGCTGGTGAT GGTGGACGTG GCGTTTTACT CGGAGAACGT TCAGGGTTTG 
CGCTCTCTGG AGCGTCTGGA CTTGAATATG CCGGAGATCT GGGAGCAGAA GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124248.1
CDS	91..3087
Translation	MSSNQGSSPIPQCSGAGGGTSDGFFRKHREPPLAPKHYRTARSLPDVCPKEPTGDGGGLCEGPSVVADQHRWTVYNSKVNLPAALNDPRLAKRESDFFTKTWGLDFSETEVMPSFYLPNITREHFSSYLQDTAQREKIHERCKNICPSKDDLSVSSITNNHEKARAELEQVPKIFMKPEFVLNDPATFNAVLPWSHFSVAGGKNSRDVASSRLLQEKLSHYLDVVEVSIARQISLRSEAFFHAMSSQHELQDQLRETADAVAKLRARTAAIDRVMCRGPLRVLRDALTRNNCIKLHNKLKLMAAVHQTQPTVQLLLSTSEFVGALELIATTKEVLQQELQGIHSFRHLGSQLCEMERLIDKMMVADFSTYAQSDLSRTFEEDGQVLEKDRLQSLVFGLLRQRKLDFLDIYSEEMIRAAKNVVRQCVVKSLSQVNEVDADTVRIHEQMRLMAFHHWFDLLLDIFEHFILFLKRIKATLGVIRNVVLEVLDSSQRSRLADATTATSHPDGRVNEDEEEESGGPALHPGEAELAYLTHEGLFISDALNDAEQRSVTTAAQTQTRAQTPRADACGSDSASATTESSSSREHNSNTTSCLPVGAAALEDVMPSDLELGRVANNVQELLYAASDVSHDRCVKVLMTRAKDGSLERLSSSEFVCLSQAVESFVKDTEELCGRRSVSLRGALQSQANRFVQRFHEERKTKLSLLLDNERWKQAEVPAEFQDLVDSIADGRISLPERKHSSGSEDRKPTEFLCVDGQKYAVVGTVLLLIRMFLEYCQCVNDIPSITTDIITRLADLLKHFNSRCCQLVLGAGALQAVGLKTITTRNLALASRCLQLVVHYIPVIRAHFETRLQPKQYSILRHFDHITKDYNDHIAEVSAKLVAIMDSMFEKAVSKYEVKAPMPSACLRNVCKQMLKMHEAIHELLPEEQTQMLFLRINASFKLHLKRQLSRLGVSNDGGPQNGLVMVDVAFYSENVQGLRSLERLDLNMPEIWEQKR

Target ORF information:

RefSeq Version	NM_001130776.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS54 subunit of GARP complex (vps54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130776.1

ORF Insert Sequence:

ATGTCCTCCA ATCAGGGCTC GTCTCCGATC CCTCAGTGCA GTGGGGCAGG AGGAGGAACC 
AGTGATGGGT TTTTCCGGAA GCACAGAGAG CCTCCACTGG CCCCCAAACA CTATCGGACG 
GCCCGCTCGC TGCCGGACGT CTGCCCTAAA GAGCCCACCG GTGATGGCGG TGGTCTGTGC 
GAGGGTCCGT CTGTTGTGGC CGATCAGCAC CGCTGGACTG TGTATAACTC TAAAGTGAAT 
CTCCCCGCGG CACTCAATGA CCCCCGGCTG GCCAAAAGAG AGTCTGACTT CTTCACTAAA 
ACCTGGGGCT TGGATTTCAG CGAGACCGAA GTCATGCCTT CATTCTACCT GCCCAACATC 
ACCCGAGAAC ACTTCAGCTC ATACCTGCAG GACACGGCTC AGAGGGAGAA GATTCACGAG 
CGCTGCAAGA ACATTTGCCC AAGCAAAGAC GATCTGTCAG TGTCCAGCAT CACTAATAAT 
CACGAAAAAG CCCGAGCTGA ACTGGAGCAG GTGCCCAAGA TCTTCATGAA GCCGGAGTTT 
GTGTTAAATG ATCCCGCCAC CTTTAATGCC GTGTTGCCGT GGTCTCACTT CAGCGTCGCC 
GGGGGAAAGA ATAGCAGAGA TGTTGCATCA TCACGGTTAC TACAGGAAAA GTTGAGTCAT 
TATCTGGACG TGGTGGAGGT GAGCATCGCT CGTCAGATCT CTCTTCGCTC CGAGGCCTTT 
TTCCACGCCA TGTCGTCTCA GCACGAGCTG CAGGACCAGC TGCGCGAGAC AGCGGACGCC 
GTGGCTAAAC TCCGCGCCCG AACCGCTGCC ATCGACCGAG TGATGTGCCG TGGGCCACTG 
CGAGTGCTAC GCGATGCTCT CACGCGCAAC AACTGCATCA AACTGCACAA CAAGCTGAAG 
CTGATGGCGG CCGTGCACCA GACGCAGCCC ACCGTACAGC TGCTGCTCTC CACCTCAGAG 
TTCGTGGGGG CGCTAGAGCT CATCGCCACC ACCAAAGAAG TGCTGCAGCA GGAACTGCAG 
GGCATTCACA GCTTCAGGCA TCTGGGTTCT CAGCTGTGTG AGATGGAGAG GCTGATCGAT 
AAGATGATGG TGGCGGATTT CTCCACTTAT GCTCAGAGTG ACCTCAGCCG AACCTTTGAG 
GAGGACGGTC AGGTTCTGGA GAAGGATCGC TTGCAGTCTC TAGTCTTTGG GCTGTTGCGG 
CAGAGGAAGC TGGATTTCCT GGACATCTAC AGTGAAGAGA TGATCCGCGC AGCTAAAAAC 
GTGGTCCGGC AGTGTGTTGT GAAGTCCCTG TCTCAGGTCA ATGAGGTCGA CGCAGACACT 
GTCAGGATTC ATGAACAGAT GCGCTTGATG GCGTTTCATC ATTGGTTTGA TCTGCTGCTG 
GATATATTTG AACACTTCAT TCTGTTCCTC AAGAGGATCA AGGCGACGCT CGGTGTCATC 
CGTAATGTTG TTCTGGAAGT GCTGGACAGC AGCCAGAGGA GTCGACTAGC GGATGCCACC 
ACAGCAACCA GTCATCCAGA TGGCAGAGTA AATGAGGATG AGGAGGAGGA GTCAGGAGGC 
CCCGCCCTTC ATCCAGGAGA GGCGGAGCTT GCGTACCTCA CTCACGAGGG TTTGTTCATC 
AGTGACGCTC TGAATGATGC AGAGCAGCGC AGTGTTACAA CAGCAGCACA AACACAGACC 
AGAGCACAGA CGCCGCGCGC AGACGCCTGC GGGAGCGACT CGGCCTCCGC AACCACTGAA 
TCCTCCTCCA GCAGGGAGCA CAATTCCAAC ACTACCTCCT GCCTGCCTGT AGGGGCCGCT 
GCGCTTGAGG ACGTCATGCC GTCTGACCTG GAGTTGGGTC GAGTGGCGAA TAACGTTCAG 
GAGCTGCTGT ATGCAGCGTC AGACGTCAGT CACGACCGCT GTGTCAAAGT GCTGATGACC 
AGAGCCAAGG ACGGCTCTCT AGAGCGCCTG AGCTCGTCAG AGTTTGTCTG TTTGAGTCAG 
GCCGTGGAGT CGTTTGTCAA AGACACTGAA GAGCTGTGCG GCAGGCGCAG TGTCAGCCTG 
AGGGGGGCGC TGCAGAGTCA GGCCAACCGC TTCGTACAGA GATTCCACGA GGAGCGCAAG 
ACCAAACTCA GTCTGCTGCT GGATAATGAG CGCTGGAAAC AGGCTGAGGT TCCTGCTGAG 
TTTCAGGATC TGGTCGACTC CATCGCTGAC GGACGAATCT CTCTGCCTGA GCGCAAACAC 
AGCAGCGGGT CCGAGGACAG GAAGCCCACT GAGTTTCTGT GTGTGGACGG ACAGAAGTAT 
GCGGTGGTCG GGACGGTACT GCTGCTGATC CGCATGTTTC TGGAGTACTG CCAGTGTGTG 
AACGACATTC CCTCCATCAC CACTGACATC ATCACACGCC TCGCCGACCT GCTCAAGCAC 
TTCAACTCTC GCTGTTGTCA GCTGGTTCTC GGTGCTGGAG CTCTGCAGGC TGTGGGACTC 
AAAACCATAA CCACCAGAAA CCTGGCTCTG GCGTCTCGCT GTCTTCAGCT GGTGGTTCAC 
TACATCCCTG TGATCAGAGC ACACTTTGAG ACGCGCCTGC AGCCCAAACA GTACAGCATA 
CTGAGACACT TTGACCACAT CACCAAGGAC TATAATGACC ACATCGCAGA GGTCTCGGCT 
AAGCTGGTGG CCATCATGGA CAGTATGTTC GAGAAGGCTG TGTCTAAGTA TGAGGTGAAG 
GCGCCGATGC CGTCTGCGTG CTTGCGTAAC GTGTGTAAGC AGATGCTGAA GATGCACGAG 
GCCATTCATG AGCTGCTGCC GGAGGAACAA ACGCAGATGC TGTTCCTCAG GATAAACGCC 
AGCTTTAAGC TCCACCTGAA GAGACAGTTG TCACGGCTGG GGGTCAGCAA TGATGGAGGA 
CCACAGAATG GGCTGGTGAT GGTGGACGTG GCGTTTTACT CGGAGAACGT TCAGGGTTTG 
CGCTCTCTGG AGCGTCTGGA CTTGAATATG CCGGAGATCT GGGAGCAGAA GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps54 ( XM_021475329.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331004.1 Danio rerio vacuolar protein sorting 54 homolog (S. cerevisiae) (vps54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147267
Clone ID Related Accession (Same CDS sequence)	XM_021475329.1
Accession Version	XM_021475329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCA TATTCCCTGT GTGTGTGAAT CAGGTCATGT GTGTGTGTTG GTTTGAGGTT 
TATGTTATGT GGGATCTTCT CATACAGATA ACAAAGCAGC CTGCGGCGTC TGGAAAGGTT 
GGTCTTGATC GCTTGCAGTC TCTAGTCTTT GGGCTGTTGC GGCAGAGGAA GCTGGATTTC 
CTGGACATCT ACAGTGAAGA GATGATCCGC GCAGCTAAAA ACGTGGTCCG GCAGTGTGTT 
GTGAAGTCCC TGTCTCAGGT CAATGAGGTC GACGCAGACA CTGTCAGGAT TCATGAACAG 
ATGCGCTTGA TGGCGTTTCA TCATTGGTTT GATCTGCTGC TGGATATATT TGAACACTTC 
ATTCTGTTCC TCAAGAGGAT CAAGGCGACG CTCAGTGTCA TCCGTAATGT TGTTCTGGAA 
GTGCTGGACA GCAGCCAGAG GAGTCGACTA GCGGATGCCA CCACAGCAAC CAGTCATCCA 
GATGGCAGAG TAAATGAGGA TGAGGAGGAG GAGTCAGGAG GCCCCGCCCT TCATCCAGGA 
GAGGCGGAGC TTGCGTACCT CACTCACGAG GGTTTGTTCA TCAGTGACGC TCTGAATGAT 
GCAGAGCAGC GCAGTGTTAC AACAGCAGCA CAAACACAGA CCAGAGCACA GACGCCGCGC 
GCAGACGCCT GCGGGAGCGA CTCGGCCTCC GCAACCACTG AATCCTCCTC CAGCAGGGAG 
CACAACTCCA ACACTACCTC CTGCCTGCCT GTAGGGGCCG CTGCGCTTGA GGACGTCATG 
CCGTCTGATC TGGAGTTGGG TCGAGTGGCG AATAATGTTC AGGAGCTGCT GTATGCAGCG 
TCAGACGTCA GTCACGACCG CTGCGTCAAA GTGCTGATGA CCAGAGCCAA GGACGGCTCT 
CTAGAGCGCC TGAGCTCGTC AGAGTTTGTC TGTTTGAGTC AGGCCGTGGA GTCGTTTGTC 
AAAGACACTG AAGAGCTGTG CGGCAGGCGC AGTGTCAGCC TGAGGGGGGC GCTGCAGAGT 
CAGGCCAACC GCTTCGTACA GAGATTCCAC GAGGAGCGCA AGACCAAACT CAGTCTGCTG 
CTGGATAATG AGCGCTGGAA ACAGGCTGAG GTTCCTGCTG AGTTTCAGGA TCTGGTCGAC 
TCCATCGCTG ACGGACGAAT CTCTCTGCCT GAGCGCAAAC ACAGCAGCGG GTCCGAGGAC 
AGGAAGCCCA CTGAGTTTCT GTGTGTGGAC GGACAGAAGT ATGCGGTGGT CGGGACGGTA 
CTGCTGCTGA TCCGCATGTT TCTGGAGTAC TGCCAGTGTG TGAACGACAT TCCCTCCATC 
ACCACTGACA TCATCACACG CCTCGCCGAC CTGCTCAAGC ACTTCAACTC TCGCTGTTGT 
CAGCTGGTTC TCGGTGCTGG AGCTCTGCAG GCTGTGGGAC TCAAAACCAT CACCACCAGA 
AACCTGGCTC TGGCATCTCG CTGTCTTCAG CTGGTGGTTC ACTACATCCC TGTGATCAGA 
GCACACTTTG AGACGCGACT GCAGCCCAAA CAGTACAGCA TACTGAGACA CTTTGACCAC 
ATCACCAAGG ACTATAATGA CCACATCGCA GAGGTCTCGG CTAAGCTGGT GGCCATCATG 
GACAGTATGT TCGAGAAGGC TCTGTCTAAG TATGAGGTGA AGGCGCCGAT GCCGTCTGCG 
TGTTTGCGTA ACGTGTGTAA GCAGATGCTG AAGATGCACG AGGCCATTCA TGAGCTGCTG 
CCGGAGGAAC AAACGCAGAT GCTGTTCCTC AGGATAAACG CCAGCTTTAA GCTCCACCTG 
AAGAGACAGT TGTCACGGCT GGGGGTCAGC AATGATGGAG GACCACAGAA TGGGCTGGTG 
ATGGTGGACG TGGCGTTTTA CTCGGAGAAC GTTCAGGGTT TGCGCTCTCT GGAGCGTCTG 
GACTTGAATA TGCCGGAGAT CTGGGAGCAG AAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331004.1
CDS	782..2740
Translation	MLSIFPVCVNQVMCVCWFEVYVMWDLLIQITKQPAASGKVGLDRLQSLVFGLLRQRKLDFLDIYSEEMIRAAKNVVRQCVVKSLSQVNEVDADTVRIHEQMRLMAFHHWFDLLLDIFEHFILFLKRIKATLSVIRNVVLEVLDSSQRSRLADATTATSHPDGRVNEDEEEESGGPALHPGEAELAYLTHEGLFISDALNDAEQRSVTTAAQTQTRAQTPRADACGSDSASATTESSSSREHNSNTTSCLPVGAAALEDVMPSDLELGRVANNVQELLYAASDVSHDRCVKVLMTRAKDGSLERLSSSEFVCLSQAVESFVKDTEELCGRRSVSLRGALQSQANRFVQRFHEERKTKLSLLLDNERWKQAEVPAEFQDLVDSIADGRISLPERKHSSGSEDRKPTEFLCVDGQKYAVVGTVLLLIRMFLEYCQCVNDIPSITTDIITRLADLLKHFNSRCCQLVLGAGALQAVGLKTITTRNLALASRCLQLVVHYIPVIRAHFETRLQPKQYSILRHFDHITKDYNDHIAEVSAKLVAIMDSMFEKALSKYEVKAPMPSACLRNVCKQMLKMHEAIHELLPEEQTQMLFLRINASFKLHLKRQLSRLGVSNDGGPQNGLVMVDVAFYSENVQGLRSLERLDLNMPEIWEQKR

Target ORF information:

RefSeq Version	XM_021475329.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vacuolar protein sorting 54 homolog (S. cerevisiae) (vps54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475329.1

ORF Insert Sequence:

ATGCTCTCCA TATTCCCTGT GTGTGTGAAT CAGGTCATGT GTGTGTGTTG GTTTGAGGTT 
TATGTTATGT GGGATCTTCT CATACAGATA ACAAAGCAGC CTGCGGCGTC TGGAAAGGTT 
GGTCTTGATC GCTTGCAGTC TCTAGTCTTT GGGCTGTTGC GGCAGAGGAA GCTGGATTTC 
CTGGACATCT ACAGTGAAGA GATGATCCGC GCAGCTAAAA ACGTGGTCCG GCAGTGTGTT 
GTGAAGTCCC TGTCTCAGGT CAATGAGGTC GACGCAGACA CTGTCAGGAT TCATGAACAG 
ATGCGCTTGA TGGCGTTTCA TCATTGGTTT GATCTGCTGC TGGATATATT TGAACACTTC 
ATTCTGTTCC TCAAGAGGAT CAAGGCGACG CTCAGTGTCA TCCGTAATGT TGTTCTGGAA 
GTGCTGGACA GCAGCCAGAG GAGTCGACTA GCGGATGCCA CCACAGCAAC CAGTCATCCA 
GATGGCAGAG TAAATGAGGA TGAGGAGGAG GAGTCAGGAG GCCCCGCCCT TCATCCAGGA 
GAGGCGGAGC TTGCGTACCT CACTCACGAG GGTTTGTTCA TCAGTGACGC TCTGAATGAT 
GCAGAGCAGC GCAGTGTTAC AACAGCAGCA CAAACACAGA CCAGAGCACA GACGCCGCGC 
GCAGACGCCT GCGGGAGCGA CTCGGCCTCC GCAACCACTG AATCCTCCTC CAGCAGGGAG 
CACAACTCCA ACACTACCTC CTGCCTGCCT GTAGGGGCCG CTGCGCTTGA GGACGTCATG 
CCGTCTGATC TGGAGTTGGG TCGAGTGGCG AATAATGTTC AGGAGCTGCT GTATGCAGCG 
TCAGACGTCA GTCACGACCG CTGCGTCAAA GTGCTGATGA CCAGAGCCAA GGACGGCTCT 
CTAGAGCGCC TGAGCTCGTC AGAGTTTGTC TGTTTGAGTC AGGCCGTGGA GTCGTTTGTC 
AAAGACACTG AAGAGCTGTG CGGCAGGCGC AGTGTCAGCC TGAGGGGGGC GCTGCAGAGT 
CAGGCCAACC GCTTCGTACA GAGATTCCAC GAGGAGCGCA AGACCAAACT CAGTCTGCTG 
CTGGATAATG AGCGCTGGAA ACAGGCTGAG GTTCCTGCTG AGTTTCAGGA TCTGGTCGAC 
TCCATCGCTG ACGGACGAAT CTCTCTGCCT GAGCGCAAAC ACAGCAGCGG GTCCGAGGAC 
AGGAAGCCCA CTGAGTTTCT GTGTGTGGAC GGACAGAAGT ATGCGGTGGT CGGGACGGTA 
CTGCTGCTGA TCCGCATGTT TCTGGAGTAC TGCCAGTGTG TGAACGACAT TCCCTCCATC 
ACCACTGACA TCATCACACG CCTCGCCGAC CTGCTCAAGC ACTTCAACTC TCGCTGTTGT 
CAGCTGGTTC TCGGTGCTGG AGCTCTGCAG GCTGTGGGAC TCAAAACCAT CACCACCAGA 
AACCTGGCTC TGGCATCTCG CTGTCTTCAG CTGGTGGTTC ACTACATCCC TGTGATCAGA 
GCACACTTTG AGACGCGACT GCAGCCCAAA CAGTACAGCA TACTGAGACA CTTTGACCAC 
ATCACCAAGG ACTATAATGA CCACATCGCA GAGGTCTCGG CTAAGCTGGT GGCCATCATG 
GACAGTATGT TCGAGAAGGC TCTGTCTAAG TATGAGGTGA AGGCGCCGAT GCCGTCTGCG 
TGTTTGCGTA ACGTGTGTAA GCAGATGCTG AAGATGCACG AGGCCATTCA TGAGCTGCTG 
CCGGAGGAAC AAACGCAGAT GCTGTTCCTC AGGATAAACG CCAGCTTTAA GCTCCACCTG 
AAGAGACAGT TGTCACGGCT GGGGGTCAGC AATGATGGAG GACCACAGAA TGGGCTGGTG 
ATGGTGGACG TGGCGTTTTA CTCGGAGAAC GTTCAGGGTT TGCGCTCTCT GGAGCGTCTG 
GACTTGAATA TGCCGGAGAT CTGGGAGCAG AAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Fish & shellfish immunology49324-35(2016 Feb)
Zhou S,Zhao H,Thongda W,Zhang D,Su B,Yu D,Peatman E,Li C