Vps54 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps54
Entrez Gene ID	245944
Full Name	VPS54 GARP complex subunit
Synonyms	Hcc8,Vps54l,mSLP8,wr
General protein information	Preferred Names VPS54 GARP complex subunit Names vacuolar protein sorting-associated protein 54 tumor antigen SLP-8p homolog vacuolar protein sorting 54
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 A3.1\|11 13.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011243720.1	XP_011242022.1	vacuolar protein sorting-associated protein 54 isoform X1
XM_030246004.1	XP_030101864.1	vacuolar protein sorting-associated protein 54 isoform X2
XM_006514699.4	XP_006514762.1	vacuolar protein sorting-associated protein 54 isoform X3
NM_139061.5	NP_620692.3	vacuolar protein sorting-associated protein 54 isoform 1
NM_001290628.1	NP_001277557.1	vacuolar protein sorting-associated protein 54 isoform 2
XM_006514699.3	XP_006514762.1	vacuolar protein sorting-associated protein 54 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.
Wei J, Zhang YY, Luo J, Wang JQ, Zhou YX, Miao HH, Shi XJ, Qu YX, Xu J, Li BL, Song BL
Cell reports19(13)2823-2835(2017 Jun)

Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant.
Gioria M, Pasini ME, Berruti G
Cell and tissue research(2017 Mar)

Steroid Profiling in Male Wobbler Mouse, a Model of Amyotrophic Lateral Sclerosis.
Gonzalez Deniselle MC, Liere P, Pianos A, Meyer M, Aprahamian F, Cambourg A, Di Giorgio NP, Schumacher M, De Nicola AF, Guennoun R
Endocrinology157(11)4446-4460(2016 Nov)

The Spatiotemporal Pattern of Degeneration in the Cerebellum of the Wobbler Mouse.
Saberi D, Ott B, Dahlke C, Matschke V, Schmitt-John T, Theiss C
Journal of neuropathology and experimental neurology75(4)347-57(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that the cerebellum is affected by neurodegenerative and inflammatory processes in the Wobbler mouse model of ALS.
Title: The Spatiotemporal Pattern of Degeneration in the Cerebellum of the Wobbler Mouse.

We conclude that a missense mutation in VPS54, an essential component of the Golgi-associated retrograde protein complex, not only prevents the formation of an acrosome but also initiates a cascade of metabolic abnormalities and a stress reaction.
Title: Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia.

Investigation of cells derived from wobbler and Vps54 null mutant embryos demonstrates impaired retrograde transport of the Cholera-toxin B subunit to the trans-Golgi network and mis-sorting of mannose-6-phosphate receptors and cargo proteins.
Title: Loss of vps54 function leads to vesicle traffic impairment, protein mis-sorting and embryonic lethality.

Severely reduced levels of mutant Vps54 and, consequently, of the whole GARP complex underlie the phenotype of the wobbler mouse.
Title: Structural basis for the wobbler mouse neurodegenerative disorder caused by mutation in the Vps54 subunit of the GARP complex.

Mutation of Vps54 causes motor neuron disease and defective spermiogenesis in the wobbler mouse
Title: Mutation of Vps54 causes motor neuron disease and defective spermiogenesis in the wobbler mouse.

The following Vps54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13959	NM_139061.5 Latest version!	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu17105	NM_001290628.1 Latest version!	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu13959	XM_011243720.1 Latest version!	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu17105	XM_030246004.1 Latest version!	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45208	XM_006514699.4 Latest version!	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu45208	XM_006514699.3	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps54 ( NM_139061.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620692.3 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13959
Clone ID Related Accession (Same CDS sequence)	XM_011243720.1 , NM_139061.5
Accession Version	NM_139061.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ134863.1, AL669979.13, AF424699.2 and AK036667.1. On Mar 15, 2014 this sequence version replaced NM_139061.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF424699.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATACT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTGTA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
ACACTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACTT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCACT CTACCTCCCA CAGATCAGCA AGGAGAATTT TATAGCATAT 
CAGCAGGAGA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGGAC TCTTTTACAT ATTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCTTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGGA AAGGAAGTCG CGACGCAGCT 
TCTTCCAAGT TGCTTCAGGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTACCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTTATGTG TGAAGGATCA CTACAGATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAAACTCAG 
CCTACAGTGC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCGTTGGA CTTAATAGCA 
ACAACTCAAG AGGTTCTGCA GCAGGAGCTT CAGGGCATTC ACAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAGCTGATT GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCATA GTGACTTAAA TAGACCACTG GAAGGTGAAT GCCAAGTTTT AGAAGAGGAA 
AGATTAGTAT CTCTTGTATT TGGGCTTTTG AAACAAAGAA AACTTAATTT TTTAGAAATC 
TATGGTGAAG AAATGATTAT TACAGCAAAG AACATCATTA AAGAGCGTGT GATTAATAAA 
GTTTCACAAA TAGAAGAAAT AGACACAGAC GTTGTTGTAA AGCTTGCAGA TCAAATGAGA 
ATGTTGAATT TTCCCCAGTG GATTGATCTG TTAAAGGACA TTTTCTCTAA GTTTACAGTT 
TTCCTACAGA GAGTTAAGGC AACATTGAAT ATTATCCACA GTGTTGTTCT GTCAGTTCTT 
GAGAAAAGCC AAAGGACTAG AGAATTGGAA GAGATCCCAC AGCAGAGGAG CGCTGGGAAG 
GACAGCTCCT TGGACACAGA CGTGGCTTAC CTGACACACG AGGGCTGGTT CATAAGTGAT 
GCTTTCAGTG AGGGTGAGCC AGCATCTGCG GCGGTTGATA CTACCTCTCA AAGAAACACG 
TCTCCACATA GCGAGCCCTG CAGCAGTGAT TCGGTATCAG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAGGAGCA GACATCAGCG TGTGCTCCTC CAGGAGGTAT AGAGATTATA 
GTCAGTGAAG ACATGAGGTT AACTGACTTG GAGCTAGGAA AACTAGCAAG TAACATCCAG 
GAGTTACTGT GTAATGCTTC AGATGTATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ACGGTTTTCT TGAAAAATTG AATTCCACGG AATTCATAGC ACTCTCGAGA 
TTAATGGAGA CATTCATAGT GGACACTGAA CAGATCTGTG GGAGGAAGAG CACGTCACTG 
CTTGGGGCCC TTCAGAGCCA AGCAAATAAG TTTGTGAACA GGTTTCATGA AGAGAGGCGG 
ACCAAGCTCA GCCTCCTCTT AGACAACGAG CGTTGGAAGC AGGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATAGCGGAT GGAAAGATTG CTTTACCTGA AAAAAAGCCA 
GTGGTTACAG AAGAAAGGAA GCCAGCTGAT GTTCTTGTTG TTGAGGGACA CCAGTATGCA 
GTTGTCGGAA CCGTCTTGCT GTTAATAAGA ATCATTCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCCT CCGTCACTAC TGACATGCTC ACTCGCCTGA CAGATCTGCT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCTT TGCAAGTTGT TGGACTAAAA 
ACAATTACTA CAAAAAACTT GGCCCTCTCT TCACGGTGTC TGCAGTTGAT TGTGCACTAC 
ATTCCCGTGA TCCGGGCTCA CTTTGAAGCC CGACTGCCCC CCAAGCAATG GAGCATGCTT 
AGACATTTTG ATCACATCAC AAAGGATTAC CATGATCATA TAGCTGAAAT TTCAGCTAAG 
CTTGTAGCGA TAATGGATAG CCTATTTGAT AAGCTGTTAT CAAAGTATGA GGTGAAAGCT 
CCTGTTCCTT CCCCGTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCATGAAGCT 
ATATTTGATC TTCTTCCCGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA ACAGTTATCA CACTTAAATG TAATAAATGA CGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATCTGGATCT AAATATGGCC GAGATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620692.3
CDS	361..3294
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSHTLCVAPSLVTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_139061.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139061.5

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATACT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTGTA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
ACACTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACTT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCACT CTACCTCCCA CAGATCAGCA AGGAGAATTT TATAGCATAT 
CAGCAGGAGA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGGAC TCTTTTACAT ATTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCTTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGGA AAGGAAGTCG CGACGCAGCT 
TCTTCCAAGT TGCTTCAGGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTACCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTTATGTG TGAAGGATCA CTACAGATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAAACTCAG 
CCTACAGTGC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCGTTGGA CTTAATAGCA 
ACAACTCAAG AGGTTCTGCA GCAGGAGCTT CAGGGCATTC ACAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAGCTGATT GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCATA GTGACTTAAA TAGACCACTG GAAGGTGAAT GCCAAGTTTT AGAAGAGGAA 
AGATTAGTAT CTCTTGTATT TGGGCTTTTG AAACAAAGAA AACTTAATTT TTTAGAAATC 
TATGGTGAAG AAATGATTAT TACAGCAAAG AACATCATTA AAGAGCGTGT GATTAATAAA 
GTTTCACAAA TAGAAGAAAT AGACACAGAC GTTGTTGTAA AGCTTGCAGA TCAAATGAGA 
ATGTTGAATT TTCCCCAGTG GATTGATCTG TTAAAGGACA TTTTCTCTAA GTTTACAGTT 
TTCCTACAGA GAGTTAAGGC AACATTGAAT ATTATCCACA GTGTTGTTCT GTCAGTTCTT 
GAGAAAAGCC AAAGGACTAG AGAATTGGAA GAGATCCCAC AGCAGAGGAG CGCTGGGAAG 
GACAGCTCCT TGGACACAGA CGTGGCTTAC CTGACACACG AGGGCTGGTT CATAAGTGAT 
GCTTTCAGTG AGGGTGAGCC AGCATCTGCG GCGGTTGATA CTACCTCTCA AAGAAACACG 
TCTCCACATA GCGAGCCCTG CAGCAGTGAT TCGGTATCAG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAGGAGCA GACATCAGCG TGTGCTCCTC CAGGAGGTAT AGAGATTATA 
GTCAGTGAAG ACATGAGGTT AACTGACTTG GAGCTAGGAA AACTAGCAAG TAACATCCAG 
GAGTTACTGT GTAATGCTTC AGATGTATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ACGGTTTTCT TGAAAAATTG AATTCCACGG AATTCATAGC ACTCTCGAGA 
TTAATGGAGA CATTCATAGT GGACACTGAA CAGATCTGTG GGAGGAAGAG CACGTCACTG 
CTTGGGGCCC TTCAGAGCCA AGCAAATAAG TTTGTGAACA GGTTTCATGA AGAGAGGCGG 
ACCAAGCTCA GCCTCCTCTT AGACAACGAG CGTTGGAAGC AGGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATAGCGGAT GGAAAGATTG CTTTACCTGA AAAAAAGCCA 
GTGGTTACAG AAGAAAGGAA GCCAGCTGAT GTTCTTGTTG TTGAGGGACA CCAGTATGCA 
GTTGTCGGAA CCGTCTTGCT GTTAATAAGA ATCATTCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCCT CCGTCACTAC TGACATGCTC ACTCGCCTGA CAGATCTGCT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCTT TGCAAGTTGT TGGACTAAAA 
ACAATTACTA CAAAAAACTT GGCCCTCTCT TCACGGTGTC TGCAGTTGAT TGTGCACTAC 
ATTCCCGTGA TCCGGGCTCA CTTTGAAGCC CGACTGCCCC CCAAGCAATG GAGCATGCTT 
AGACATTTTG ATCACATCAC AAAGGATTAC CATGATCATA TAGCTGAAAT TTCAGCTAAG 
CTTGTAGCGA TAATGGATAG CCTATTTGAT AAGCTGTTAT CAAAGTATGA GGTGAAAGCT 
CCTGTTCCTT CCCCGTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCATGAAGCT 
ATATTTGATC TTCTTCCCGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA ACAGTTATCA CACTTAAATG TAATAAATGA CGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATCTGGATCT AAATATGGCC GAGATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps54 ( NM_001290628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277557.1 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17105
Clone ID Related Accession (Same CDS sequence)	XM_030246004.1 , NM_001290628.1
Accession Version	NM_001290628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ134863.1, AL669979.13, AF424699.2 and AK036667.1. On Mar 15, 2014 this sequence version replaced XM_006514698.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.120458.1, BC025012.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TGTATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTACACTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACTTGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CACTCTACCT CCCACAGATC 
AGCAAGGAGA ATTTTATAGC ATATCAGCAG GAGATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GGACTCTTTT ACATATTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CTTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGGAAAGGAA GTCGCGACGC AGCTTCTTCC AAGTTGCTTC AGGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTAC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTTA TGTGTGAAGG ATCACTACAG 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAAAC TCAGCCTACA GTGCAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCGT TGGACTTAAT AGCAACAACT CAAGAGGTTC TGCAGCAGGA GCTTCAGGGC 
ATTCACAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAGCT GATTGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CATAGTGACT TAAATAGACC ACTGGAAGGT 
GAATGCCAAG TTTTAGAAGA GGAAAGATTA GTATCTCTTG TATTTGGGCT TTTGAAACAA 
AGAAAACTTA ATTTTTTAGA AATCTATGGT GAAGAAATGA TTATTACAGC AAAGAACATC 
ATTAAAGAGC GTGTGATTAA TAAAGTTTCA CAAATAGAAG AAATAGACAC AGACGTTGTT 
GTAAAGCTTG CAGATCAAAT GAGAATGTTG AATTTTCCCC AGTGGATTGA TCTGTTAAAG 
GACATTTTCT CTAAGTTTAC AGTTTTCCTA CAGAGAGTTA AGGCAACATT GAATATTATC 
CACAGTGTTG TTCTGTCAGT TCTTGAGAAA AGCCAAAGGA CTAGAGAATT GGAAGAGATC 
CCACAGCAGA GGAGCGCTGG GAAGGACAGC TCCTTGGACA CAGACGTGGC TTACCTGACA 
CACGAGGGCT GGTTCATAAG TGATGCTTTC AGTGAGGGTG AGCCAGCATC TGCGGCGGTT 
GATACTACCT CTCAAAGAAA CACGTCTCCA CATAGCGAGC CCTGCAGCAG TGATTCGGTA 
TCAGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAGG AGCAGACATC AGCGTGTGCT 
CCTCCAGGAG GTATAGAGAT TATAGTCAGT GAAGACATGA GGTTAACTGA CTTGGAGCTA 
GGAAAACTAG CAAGTAACAT CCAGGAGTTA CTGTGTAATG CTTCAGATGT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGACGGTT TTCTTGAAAA ATTGAATTCC 
ACGGAATTCA TAGCACTCTC GAGATTAATG GAGACATTCA TAGTGGACAC TGAACAGATC 
TGTGGGAGGA AGAGCACGTC ACTGCTTGGG GCCCTTCAGA GCCAAGCAAA TAAGTTTGTG 
AACAGGTTTC ATGAAGAGAG GCGGACCAAG CTCAGCCTCC TCTTAGACAA CGAGCGTTGG 
AAGCAGGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATAGC GGATGGAAAG 
ATTGCTTTAC CTGAAAAAAA GCCAGTGGTT ACAGAAGAAA GGAAGCCAGC TGATGTTCTT 
GTTGTTGAGG GACACCAGTA TGCAGTTGTC GGAACCGTCT TGCTGTTAAT AAGAATCATT 
CTTGAATATT GCCAGTGTGT GGATAACATC CCCTCCGTCA CTACTGACAT GCTCACTCGC 
CTGACAGATC TGCTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCTTTGCAAG TTGTTGGACT AAAAACAATT ACTACAAAAA ACTTGGCCCT CTCTTCACGG 
TGTCTGCAGT TGATTGTGCA CTACATTCCC GTGATCCGGG CTCACTTTGA AGCCCGACTG 
CCCCCCAAGC AATGGAGCAT GCTTAGACAT TTTGATCACA TCACAAAGGA TTACCATGAT 
CATATAGCTG AAATTTCAGC TAAGCTTGTA GCGATAATGG ATAGCCTATT TGATAAGCTG 
TTATCAAAGT ATGAGGTGAA AGCTCCTGTT CCTTCCCCGT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCATGA AGCTATATTT GATCTTCTTC CCGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAACAGTT ATCACACTTA 
AATGTAATAA ATGACGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATCTGG ATCTAAATAT GGCCGAGATC 
TGGGAACAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277557.1
CDS	361..3258
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_001290628.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290628.1

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TGTATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTACACTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACTTGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CACTCTACCT CCCACAGATC 
AGCAAGGAGA ATTTTATAGC ATATCAGCAG GAGATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GGACTCTTTT ACATATTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CTTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGGAAAGGAA GTCGCGACGC AGCTTCTTCC AAGTTGCTTC AGGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTAC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTTA TGTGTGAAGG ATCACTACAG 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAAAC TCAGCCTACA GTGCAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCGT TGGACTTAAT AGCAACAACT CAAGAGGTTC TGCAGCAGGA GCTTCAGGGC 
ATTCACAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAGCT GATTGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CATAGTGACT TAAATAGACC ACTGGAAGGT 
GAATGCCAAG TTTTAGAAGA GGAAAGATTA GTATCTCTTG TATTTGGGCT TTTGAAACAA 
AGAAAACTTA ATTTTTTAGA AATCTATGGT GAAGAAATGA TTATTACAGC AAAGAACATC 
ATTAAAGAGC GTGTGATTAA TAAAGTTTCA CAAATAGAAG AAATAGACAC AGACGTTGTT 
GTAAAGCTTG CAGATCAAAT GAGAATGTTG AATTTTCCCC AGTGGATTGA TCTGTTAAAG 
GACATTTTCT CTAAGTTTAC AGTTTTCCTA CAGAGAGTTA AGGCAACATT GAATATTATC 
CACAGTGTTG TTCTGTCAGT TCTTGAGAAA AGCCAAAGGA CTAGAGAATT GGAAGAGATC 
CCACAGCAGA GGAGCGCTGG GAAGGACAGC TCCTTGGACA CAGACGTGGC TTACCTGACA 
CACGAGGGCT GGTTCATAAG TGATGCTTTC AGTGAGGGTG AGCCAGCATC TGCGGCGGTT 
GATACTACCT CTCAAAGAAA CACGTCTCCA CATAGCGAGC CCTGCAGCAG TGATTCGGTA 
TCAGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAGG AGCAGACATC AGCGTGTGCT 
CCTCCAGGAG GTATAGAGAT TATAGTCAGT GAAGACATGA GGTTAACTGA CTTGGAGCTA 
GGAAAACTAG CAAGTAACAT CCAGGAGTTA CTGTGTAATG CTTCAGATGT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGACGGTT TTCTTGAAAA ATTGAATTCC 
ACGGAATTCA TAGCACTCTC GAGATTAATG GAGACATTCA TAGTGGACAC TGAACAGATC 
TGTGGGAGGA AGAGCACGTC ACTGCTTGGG GCCCTTCAGA GCCAAGCAAA TAAGTTTGTG 
AACAGGTTTC ATGAAGAGAG GCGGACCAAG CTCAGCCTCC TCTTAGACAA CGAGCGTTGG 
AAGCAGGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATAGC GGATGGAAAG 
ATTGCTTTAC CTGAAAAAAA GCCAGTGGTT ACAGAAGAAA GGAAGCCAGC TGATGTTCTT 
GTTGTTGAGG GACACCAGTA TGCAGTTGTC GGAACCGTCT TGCTGTTAAT AAGAATCATT 
CTTGAATATT GCCAGTGTGT GGATAACATC CCCTCCGTCA CTACTGACAT GCTCACTCGC 
CTGACAGATC TGCTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCTTTGCAAG TTGTTGGACT AAAAACAATT ACTACAAAAA ACTTGGCCCT CTCTTCACGG 
TGTCTGCAGT TGATTGTGCA CTACATTCCC GTGATCCGGG CTCACTTTGA AGCCCGACTG 
CCCCCCAAGC AATGGAGCAT GCTTAGACAT TTTGATCACA TCACAAAGGA TTACCATGAT 
CATATAGCTG AAATTTCAGC TAAGCTTGTA GCGATAATGG ATAGCCTATT TGATAAGCTG 
TTATCAAAGT ATGAGGTGAA AGCTCCTGTT CCTTCCCCGT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCATGA AGCTATATTT GATCTTCTTC CCGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAACAGTT ATCACACTTA 
AATGTAATAA ATGACGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATCTGG ATCTAAATAT GGCCGAGATC 
TGGGAACAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps54 ( XM_011243720.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242022.1 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13959
Clone ID Related Accession (Same CDS sequence)	XM_011243720.1 , NM_139061.5
Accession Version	XM_011243720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATACT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTGTA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
ACACTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACTT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCACT CTACCTCCCA CAGATCAGCA AGGAGAATTT TATAGCATAT 
CAGCAGGAGA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGGAC TCTTTTACAT ATTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCTTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGGA AAGGAAGTCG CGACGCAGCT 
TCTTCCAAGT TGCTTCAGGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTACCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTTATGTG TGAAGGATCA CTACAGATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAAACTCAG 
CCTACAGTGC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCGTTGGA CTTAATAGCA 
ACAACTCAAG AGGTTCTGCA GCAGGAGCTT CAGGGCATTC ACAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAGCTGATT GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCATA GTGACTTAAA TAGACCACTG GAAGGTGAAT GCCAAGTTTT AGAAGAGGAA 
AGATTAGTAT CTCTTGTATT TGGGCTTTTG AAACAAAGAA AACTTAATTT TTTAGAAATC 
TATGGTGAAG AAATGATTAT TACAGCAAAG AACATCATTA AAGAGCGTGT GATTAATAAA 
GTTTCACAAA TAGAAGAAAT AGACACAGAC GTTGTTGTAA AGCTTGCAGA TCAAATGAGA 
ATGTTGAATT TTCCCCAGTG GATTGATCTG TTAAAGGACA TTTTCTCTAA GTTTACAGTT 
TTCCTACAGA GAGTTAAGGC AACATTGAAT ATTATCCACA GTGTTGTTCT GTCAGTTCTT 
GAGAAAAGCC AAAGGACTAG AGAATTGGAA GAGATCCCAC AGCAGAGGAG CGCTGGGAAG 
GACAGCTCCT TGGACACAGA CGTGGCTTAC CTGACACACG AGGGCTGGTT CATAAGTGAT 
GCTTTCAGTG AGGGTGAGCC AGCATCTGCG GCGGTTGATA CTACCTCTCA AAGAAACACG 
TCTCCACATA GCGAGCCCTG CAGCAGTGAT TCGGTATCAG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAGGAGCA GACATCAGCG TGTGCTCCTC CAGGAGGTAT AGAGATTATA 
GTCAGTGAAG ACATGAGGTT AACTGACTTG GAGCTAGGAA AACTAGCAAG TAACATCCAG 
GAGTTACTGT GTAATGCTTC AGATGTATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ACGGTTTTCT TGAAAAATTG AATTCCACGG AATTCATAGC ACTCTCGAGA 
TTAATGGAGA CATTCATAGT GGACACTGAA CAGATCTGTG GGAGGAAGAG CACGTCACTG 
CTTGGGGCCC TTCAGAGCCA AGCAAATAAG TTTGTGAACA GGTTTCATGA AGAGAGGCGG 
ACCAAGCTCA GCCTCCTCTT AGACAACGAG CGTTGGAAGC AGGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATAGCGGAT GGAAAGATTG CTTTACCTGA AAAAAAGCCA 
GTGGTTACAG AAGAAAGGAA GCCAGCTGAT GTTCTTGTTG TTGAGGGACA CCAGTATGCA 
GTTGTCGGAA CCGTCTTGCT GTTAATAAGA ATCATTCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCCT CCGTCACTAC TGACATGCTC ACTCGCCTGA CAGATCTGCT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCTT TGCAAGTTGT TGGACTAAAA 
ACAATTACTA CAAAAAACTT GGCCCTCTCT TCACGGTGTC TGCAGTTGAT TGTGCACTAC 
ATTCCCGTGA TCCGGGCTCA CTTTGAAGCC CGACTGCCCC CCAAGCAATG GAGCATGCTT 
AGACATTTTG ATCACATCAC AAAGGATTAC CATGATCATA TAGCTGAAAT TTCAGCTAAG 
CTTGTAGCGA TAATGGATAG CCTATTTGAT AAGCTGTTAT CAAAGTATGA GGTGAAAGCT 
CCTGTTCCTT CCCCGTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCATGAAGCT 
ATATTTGATC TTCTTCCCGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA ACAGTTATCA CACTTAAATG TAATAAATGA CGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATCTGGATCT AAATATGGCC GAGATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242022.1
CDS	54..2987
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSHTLCVAPSLVTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_011243720.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243720.1

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCACATACT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTGTGTA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
ACACTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACTT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCACT CTACCTCCCA CAGATCAGCA AGGAGAATTT TATAGCATAT 
CAGCAGGAGA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGGAC TCTTTTACAT ATTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCTTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGGA AAGGAAGTCG CGACGCAGCT 
TCTTCCAAGT TGCTTCAGGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTACCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTTATGTG TGAAGGATCA CTACAGATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAAACTCAG 
CCTACAGTGC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCGTTGGA CTTAATAGCA 
ACAACTCAAG AGGTTCTGCA GCAGGAGCTT CAGGGCATTC ACAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAGCTGATT GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCATA GTGACTTAAA TAGACCACTG GAAGGTGAAT GCCAAGTTTT AGAAGAGGAA 
AGATTAGTAT CTCTTGTATT TGGGCTTTTG AAACAAAGAA AACTTAATTT TTTAGAAATC 
TATGGTGAAG AAATGATTAT TACAGCAAAG AACATCATTA AAGAGCGTGT GATTAATAAA 
GTTTCACAAA TAGAAGAAAT AGACACAGAC GTTGTTGTAA AGCTTGCAGA TCAAATGAGA 
ATGTTGAATT TTCCCCAGTG GATTGATCTG TTAAAGGACA TTTTCTCTAA GTTTACAGTT 
TTCCTACAGA GAGTTAAGGC AACATTGAAT ATTATCCACA GTGTTGTTCT GTCAGTTCTT 
GAGAAAAGCC AAAGGACTAG AGAATTGGAA GAGATCCCAC AGCAGAGGAG CGCTGGGAAG 
GACAGCTCCT TGGACACAGA CGTGGCTTAC CTGACACACG AGGGCTGGTT CATAAGTGAT 
GCTTTCAGTG AGGGTGAGCC AGCATCTGCG GCGGTTGATA CTACCTCTCA AAGAAACACG 
TCTCCACATA GCGAGCCCTG CAGCAGTGAT TCGGTATCAG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAGGAGCA GACATCAGCG TGTGCTCCTC CAGGAGGTAT AGAGATTATA 
GTCAGTGAAG ACATGAGGTT AACTGACTTG GAGCTAGGAA AACTAGCAAG TAACATCCAG 
GAGTTACTGT GTAATGCTTC AGATGTATGC CATGATCGAG CTGTCAAATT TCTCATGTCA 
AGAGCAAAGG ACGGTTTTCT TGAAAAATTG AATTCCACGG AATTCATAGC ACTCTCGAGA 
TTAATGGAGA CATTCATAGT GGACACTGAA CAGATCTGTG GGAGGAAGAG CACGTCACTG 
CTTGGGGCCC TTCAGAGCCA AGCAAATAAG TTTGTGAACA GGTTTCATGA AGAGAGGCGG 
ACCAAGCTCA GCCTCCTCTT AGACAACGAG CGTTGGAAGC AGGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATAGCGGAT GGAAAGATTG CTTTACCTGA AAAAAAGCCA 
GTGGTTACAG AAGAAAGGAA GCCAGCTGAT GTTCTTGTTG TTGAGGGACA CCAGTATGCA 
GTTGTCGGAA CCGTCTTGCT GTTAATAAGA ATCATTCTTG AATATTGCCA GTGTGTGGAT 
AACATCCCCT CCGTCACTAC TGACATGCTC ACTCGCCTGA CAGATCTGCT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCTT TGCAAGTTGT TGGACTAAAA 
ACAATTACTA CAAAAAACTT GGCCCTCTCT TCACGGTGTC TGCAGTTGAT TGTGCACTAC 
ATTCCCGTGA TCCGGGCTCA CTTTGAAGCC CGACTGCCCC CCAAGCAATG GAGCATGCTT 
AGACATTTTG ATCACATCAC AAAGGATTAC CATGATCATA TAGCTGAAAT TTCAGCTAAG 
CTTGTAGCGA TAATGGATAG CCTATTTGAT AAGCTGTTAT CAAAGTATGA GGTGAAAGCT 
CCTGTTCCTT CCCCGTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCATGAAGCT 
ATATTTGATC TTCTTCCCGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA ACAGTTATCA CACTTAAATG TAATAAATGA CGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTAAAG ATCTGGATCT AAATATGGCC GAGATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps54 ( XM_006514699.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514762.1 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45208
Clone ID Related Accession (Same CDS sequence)	XM_006514699.4 , XM_006514699.3
Accession Version	XM_006514699.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG ATTTTGCCTT GGATGATTCT TTAACTTTTA ATTCAGTTTT ACCATGGTCT 
CATTTTAATA CTGCTGGTGG GAAAGGAAGT CGCGACGCAG CTTCTTCCAA GTTGCTTCAG 
GAAAAGCTGA GCCATTATCT GGATATTGTG GAAGTAAACA TTGCTCACCA GATCTCTCTA 
CGTTCAGAAG CATTTTTTCA TGCAATGACC TCTCAACACG AATTGCAGGA CTACCTCAAG 
AAAACTACCC AGGCTGTAAA AATGCTTCGA GATAAAATTG CACAGATTGA TAAAGTTATG 
TGTGAAGGAT CACTACAGAT TTTAAGACTG GCACTTACCA GAAATAATTG TGTTAAAGTA 
TACAATAAAC TGAAGTTAAT GGCCACTGTA CACCAAACTC AGCCTACAGT GCAGGTGTTG 
TTATCTACTT CTGAATTTGT TGGAGCGTTG GACTTAATAG CAACAACTCA AGAGGTTCTG 
CAGCAGGAGC TTCAGGGCAT TCACAGCTTC CGGCATTTGG GATCACAGCT TTGTGAATTA 
GAAAAGCTGA TTGATAAAAT GATGATTGCA GAATTTTCTA CTTATTCTCA TAGTGACTTA 
AATAGACCAC TGGAAGGTGA ATGCCAAGTT TTAGAAGAGG AAAGATTAGT ATCTCTTGTA 
TTTGGGCTTT TGAAACAAAG AAAACTTAAT TTTTTAGAAA TCTATGGTGA AGAAATGATT 
ATTACAGCAA AGAACATCAT TAAAGAGCGT GTGATTAATA AAGTTTCACA AATAGAAGAA 
ATAGACACAG ACGTTGTTGT AAAGCTTGCA GATCAAATGA GAATGTTGAA TTTTCCCCAG 
TGGATTGATC TGTTAAAGGA CATTTTCTCT AAGTTTACAG TTTTCCTACA GAGAGTTAAG 
GCAACATTGA ATATTATCCA CAGTGTTGTT CTGTCAGTTC TTGAGAAAAG CCAAAGGACT 
AGAGAATTGG AAGAGATCCC ACAGCAGAGG AGCGCTGGGA AGGACAGCTC CTTGGACACA 
GACGTGGCTT ACCTGACACA CGAGGGCTGG TTCATAAGTG ATGCTTTCAG TGAGGGTGAG 
CCAGCATCTG CGGCGGTTGA TACTACCTCT CAAAGAAACA CGTCTCCACA TAGCGAGCCC 
TGCAGCAGTG ATTCGGTATC AGAACCAGAA TGTACTACTG ATTCTTCATC CAGCAAGGAG 
CAGACATCAG CGTGTGCTCC TCCAGGAGGT ATAGAGATTA TAGTCAGTGA AGACATGAGG 
TTAACTGACT TGGAGCTAGG AAAACTAGCA AGTAACATCC AGGAGTTACT GTGTAATGCT 
TCAGATGTAT GCCATGATCG AGCTGTCAAA TTTCTCATGT CAAGAGCAAA GGACGGTTTT 
CTTGAAAAAT TGAATTCCAC GGAATTCATA GCACTCTCGA GATTAATGGA GACATTCATA 
GTGGACACTG AACAGATCTG TGGGAGGAAG AGCACGTCAC TGCTTGGGGC CCTTCAGAGC 
CAAGCAAATA AGTTTGTGAA CAGGTTTCAT GAAGAGAGGC GGACCAAGCT CAGCCTCCTC 
TTAGACAACG AGCGTTGGAA GCAGGCTGAT GTTCCTGCAG AATTTCAGGA TCTTGTTGAT 
TCTATAGCGG ATGGAAAGAT TGCTTTACCT GAAAAAAAGC CAGTGGTTAC AGAAGAAAGG 
AAGCCAGCTG ATGTTCTTGT TGTTGAGGGA CACCAGTATG CAGTTGTCGG AACCGTCTTG 
CTGTTAATAA GAATCATTCT TGAATATTGC CAGTGTGTGG ATAACATCCC CTCCGTCACT 
ACTGACATGC TCACTCGCCT GACAGATCTG CTGAAGTACT TCAATTCAAG AAGTTGCCAG 
TTAGTTCTTG GAGCTGGTGC TTTGCAAGTT GTTGGACTAA AAACAATTAC TACAAAAAAC 
TTGGCCCTCT CTTCACGGTG TCTGCAGTTG ATTGTGCACT ACATTCCCGT GATCCGGGCT 
CACTTTGAAG CCCGACTGCC CCCCAAGCAA TGGAGCATGC TTAGACATTT TGATCACATC 
ACAAAGGATT ACCATGATCA TATAGCTGAA ATTTCAGCTA AGCTTGTAGC GATAATGGAT 
AGCCTATTTG ATAAGCTGTT ATCAAAGTAT GAGGTGAAAG CTCCTGTTCC TTCCCCGTGT 
TTCAGGAATA TTTGTAAGCA AATGACAAAA ATGCATGAAG CTATATTTGA TCTTCTTCCC 
GAAGAACAAA CACAGATGTT ATTTTTAAGA ATTAATGCAA GTTACAAACT CCACTTGAAA 
AAACAGTTAT CACACTTAAA TGTAATAAAT GACGGAGGAC CTCAAAATGG GTTGGTCACA 
GCAGATGTAG CTTTTTACAC TGGAAATCTT CAAGCCTTAA AAGGCCTTAA AGATCTGGAT 
CTAAATATGG CCGAGATCTG GGAACAGAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514762.1
CDS	105..2540
Translation	MKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_006514699.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514699.3

ORF Insert Sequence:

ATGAAACCAG ATTTTGCCTT GGATGATTCT TTAACTTTTA ATTCAGTTTT ACCATGGTCT 
CATTTTAATA CTGCTGGTGG GAAAGGAAGT CGCGACGCAG CTTCTTCCAA GTTGCTTCAG 
GAAAAGCTGA GCCATTATCT GGATATTGTG GAAGTAAACA TTGCTCACCA GATCTCTCTA 
CGTTCAGAAG CATTTTTTCA TGCAATGACC TCTCAACACG AATTGCAGGA CTACCTCAAG 
AAAACTACCC AGGCTGTAAA AATGCTTCGA GATAAAATTG CACAGATTGA TAAAGTTATG 
TGTGAAGGAT CACTACAGAT TTTAAGACTG GCACTTACCA GAAATAATTG TGTTAAAGTA 
TACAATAAAC TGAAGTTAAT GGCCACTGTA CACCAAACTC AGCCTACAGT GCAGGTGTTG 
TTATCTACTT CTGAATTTGT TGGAGCGTTG GACTTAATAG CAACAACTCA AGAGGTTCTG 
CAGCAGGAGC TTCAGGGCAT TCACAGCTTC CGGCATTTGG GATCACAGCT TTGTGAATTA 
GAAAAGCTGA TTGATAAAAT GATGATTGCA GAATTTTCTA CTTATTCTCA TAGTGACTTA 
AATAGACCAC TGGAAGGTGA ATGCCAAGTT TTAGAAGAGG AAAGATTAGT ATCTCTTGTA 
TTTGGGCTTT TGAAACAAAG AAAACTTAAT TTTTTAGAAA TCTATGGTGA AGAAATGATT 
ATTACAGCAA AGAACATCAT TAAAGAGCGT GTGATTAATA AAGTTTCACA AATAGAAGAA 
ATAGACACAG ACGTTGTTGT AAAGCTTGCA GATCAAATGA GAATGTTGAA TTTTCCCCAG 
TGGATTGATC TGTTAAAGGA CATTTTCTCT AAGTTTACAG TTTTCCTACA GAGAGTTAAG 
GCAACATTGA ATATTATCCA CAGTGTTGTT CTGTCAGTTC TTGAGAAAAG CCAAAGGACT 
AGAGAATTGG AAGAGATCCC ACAGCAGAGG AGCGCTGGGA AGGACAGCTC CTTGGACACA 
GACGTGGCTT ACCTGACACA CGAGGGCTGG TTCATAAGTG ATGCTTTCAG TGAGGGTGAG 
CCAGCATCTG CGGCGGTTGA TACTACCTCT CAAAGAAACA CGTCTCCACA TAGCGAGCCC 
TGCAGCAGTG ATTCGGTATC AGAACCAGAA TGTACTACTG ATTCTTCATC CAGCAAGGAG 
CAGACATCAG CGTGTGCTCC TCCAGGAGGT ATAGAGATTA TAGTCAGTGA AGACATGAGG 
TTAACTGACT TGGAGCTAGG AAAACTAGCA AGTAACATCC AGGAGTTACT GTGTAATGCT 
TCAGATGTAT GCCATGATCG AGCTGTCAAA TTTCTCATGT CAAGAGCAAA GGACGGTTTT 
CTTGAAAAAT TGAATTCCAC GGAATTCATA GCACTCTCGA GATTAATGGA GACATTCATA 
GTGGACACTG AACAGATCTG TGGGAGGAAG AGCACGTCAC TGCTTGGGGC CCTTCAGAGC 
CAAGCAAATA AGTTTGTGAA CAGGTTTCAT GAAGAGAGGC GGACCAAGCT CAGCCTCCTC 
TTAGACAACG AGCGTTGGAA GCAGGCTGAT GTTCCTGCAG AATTTCAGGA TCTTGTTGAT 
TCTATAGCGG ATGGAAAGAT TGCTTTACCT GAAAAAAAGC CAGTGGTTAC AGAAGAAAGG 
AAGCCAGCTG ATGTTCTTGT TGTTGAGGGA CACCAGTATG CAGTTGTCGG AACCGTCTTG 
CTGTTAATAA GAATCATTCT TGAATATTGC CAGTGTGTGG ATAACATCCC CTCCGTCACT 
ACTGACATGC TCACTCGCCT GACAGATCTG CTGAAGTACT TCAATTCAAG AAGTTGCCAG 
TTAGTTCTTG GAGCTGGTGC TTTGCAAGTT GTTGGACTAA AAACAATTAC TACAAAAAAC 
TTGGCCCTCT CTTCACGGTG TCTGCAGTTG ATTGTGCACT ACATTCCCGT GATCCGGGCT 
CACTTTGAAG CCCGACTGCC CCCCAAGCAA TGGAGCATGC TTAGACATTT TGATCACATC 
ACAAAGGATT ACCATGATCA TATAGCTGAA ATTTCAGCTA AGCTTGTAGC GATAATGGAT 
AGCCTATTTG ATAAGCTGTT ATCAAAGTAT GAGGTGAAAG CTCCTGTTCC TTCCCCGTGT 
TTCAGGAATA TTTGTAAGCA AATGACAAAA ATGCATGAAG CTATATTTGA TCTTCTTCCC 
GAAGAACAAA CACAGATGTT ATTTTTAAGA ATTAATGCAA GTTACAAACT CCACTTGAAA 
AAACAGTTAT CACACTTAAA TGTAATAAAT GACGGAGGAC CTCAAAATGG GTTGGTCACA 
GCAGATGTAG CTTTTTACAC TGGAAATCTT CAAGCCTTAA AAGGCCTTAA AGATCTGGAT 
CTAAATATGG CCGAGATCTG GGAACAGAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps54 ( XM_030246004.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101864.1 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu17105
Clone ID Related Accession (Same CDS sequence)	XM_030246004.1 , NM_001290628.1
Accession Version	XM_030246004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TGTATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTACACTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACTTGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CACTCTACCT CCCACAGATC 
AGCAAGGAGA ATTTTATAGC ATATCAGCAG GAGATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GGACTCTTTT ACATATTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CTTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGGAAAGGAA GTCGCGACGC AGCTTCTTCC AAGTTGCTTC AGGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTAC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTTA TGTGTGAAGG ATCACTACAG 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAAAC TCAGCCTACA GTGCAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCGT TGGACTTAAT AGCAACAACT CAAGAGGTTC TGCAGCAGGA GCTTCAGGGC 
ATTCACAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAGCT GATTGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CATAGTGACT TAAATAGACC ACTGGAAGGT 
GAATGCCAAG TTTTAGAAGA GGAAAGATTA GTATCTCTTG TATTTGGGCT TTTGAAACAA 
AGAAAACTTA ATTTTTTAGA AATCTATGGT GAAGAAATGA TTATTACAGC AAAGAACATC 
ATTAAAGAGC GTGTGATTAA TAAAGTTTCA CAAATAGAAG AAATAGACAC AGACGTTGTT 
GTAAAGCTTG CAGATCAAAT GAGAATGTTG AATTTTCCCC AGTGGATTGA TCTGTTAAAG 
GACATTTTCT CTAAGTTTAC AGTTTTCCTA CAGAGAGTTA AGGCAACATT GAATATTATC 
CACAGTGTTG TTCTGTCAGT TCTTGAGAAA AGCCAAAGGA CTAGAGAATT GGAAGAGATC 
CCACAGCAGA GGAGCGCTGG GAAGGACAGC TCCTTGGACA CAGACGTGGC TTACCTGACA 
CACGAGGGCT GGTTCATAAG TGATGCTTTC AGTGAGGGTG AGCCAGCATC TGCGGCGGTT 
GATACTACCT CTCAAAGAAA CACGTCTCCA CATAGCGAGC CCTGCAGCAG TGATTCGGTA 
TCAGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAGG AGCAGACATC AGCGTGTGCT 
CCTCCAGGAG GTATAGAGAT TATAGTCAGT GAAGACATGA GGTTAACTGA CTTGGAGCTA 
GGAAAACTAG CAAGTAACAT CCAGGAGTTA CTGTGTAATG CTTCAGATGT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGACGGTT TTCTTGAAAA ATTGAATTCC 
ACGGAATTCA TAGCACTCTC GAGATTAATG GAGACATTCA TAGTGGACAC TGAACAGATC 
TGTGGGAGGA AGAGCACGTC ACTGCTTGGG GCCCTTCAGA GCCAAGCAAA TAAGTTTGTG 
AACAGGTTTC ATGAAGAGAG GCGGACCAAG CTCAGCCTCC TCTTAGACAA CGAGCGTTGG 
AAGCAGGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATAGC GGATGGAAAG 
ATTGCTTTAC CTGAAAAAAA GCCAGTGGTT ACAGAAGAAA GGAAGCCAGC TGATGTTCTT 
GTTGTTGAGG GACACCAGTA TGCAGTTGTC GGAACCGTCT TGCTGTTAAT AAGAATCATT 
CTTGAATATT GCCAGTGTGT GGATAACATC CCCTCCGTCA CTACTGACAT GCTCACTCGC 
CTGACAGATC TGCTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCTTTGCAAG TTGTTGGACT AAAAACAATT ACTACAAAAA ACTTGGCCCT CTCTTCACGG 
TGTCTGCAGT TGATTGTGCA CTACATTCCC GTGATCCGGG CTCACTTTGA AGCCCGACTG 
CCCCCCAAGC AATGGAGCAT GCTTAGACAT TTTGATCACA TCACAAAGGA TTACCATGAT 
CATATAGCTG AAATTTCAGC TAAGCTTGTA GCGATAATGG ATAGCCTATT TGATAAGCTG 
TTATCAAAGT ATGAGGTGAA AGCTCCTGTT CCTTCCCCGT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCATGA AGCTATATTT GATCTTCTTC CCGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAACAGTT ATCACACTTA 
AATGTAATAA ATGACGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATCTGG ATCTAAATAT GGCCGAGATC 
TGGGAACAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101864.1
CDS	8447..11344
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_030246004.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246004.1

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCTCCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGATCCAAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TGTATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTACACTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACTTGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CACTCTACCT CCCACAGATC 
AGCAAGGAGA ATTTTATAGC ATATCAGCAG GAGATCTCTC AGAGAGAGAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GGACTCTTTT ACATATTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGATGATT CTTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGGAAAGGAA GTCGCGACGC AGCTTCTTCC AAGTTGCTTC AGGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTAC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTTA TGTGTGAAGG ATCACTACAG 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAAAC TCAGCCTACA GTGCAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCGT TGGACTTAAT AGCAACAACT CAAGAGGTTC TGCAGCAGGA GCTTCAGGGC 
ATTCACAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAGCT GATTGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CATAGTGACT TAAATAGACC ACTGGAAGGT 
GAATGCCAAG TTTTAGAAGA GGAAAGATTA GTATCTCTTG TATTTGGGCT TTTGAAACAA 
AGAAAACTTA ATTTTTTAGA AATCTATGGT GAAGAAATGA TTATTACAGC AAAGAACATC 
ATTAAAGAGC GTGTGATTAA TAAAGTTTCA CAAATAGAAG AAATAGACAC AGACGTTGTT 
GTAAAGCTTG CAGATCAAAT GAGAATGTTG AATTTTCCCC AGTGGATTGA TCTGTTAAAG 
GACATTTTCT CTAAGTTTAC AGTTTTCCTA CAGAGAGTTA AGGCAACATT GAATATTATC 
CACAGTGTTG TTCTGTCAGT TCTTGAGAAA AGCCAAAGGA CTAGAGAATT GGAAGAGATC 
CCACAGCAGA GGAGCGCTGG GAAGGACAGC TCCTTGGACA CAGACGTGGC TTACCTGACA 
CACGAGGGCT GGTTCATAAG TGATGCTTTC AGTGAGGGTG AGCCAGCATC TGCGGCGGTT 
GATACTACCT CTCAAAGAAA CACGTCTCCA CATAGCGAGC CCTGCAGCAG TGATTCGGTA 
TCAGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAGG AGCAGACATC AGCGTGTGCT 
CCTCCAGGAG GTATAGAGAT TATAGTCAGT GAAGACATGA GGTTAACTGA CTTGGAGCTA 
GGAAAACTAG CAAGTAACAT CCAGGAGTTA CTGTGTAATG CTTCAGATGT ATGCCATGAT 
CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGACGGTT TTCTTGAAAA ATTGAATTCC 
ACGGAATTCA TAGCACTCTC GAGATTAATG GAGACATTCA TAGTGGACAC TGAACAGATC 
TGTGGGAGGA AGAGCACGTC ACTGCTTGGG GCCCTTCAGA GCCAAGCAAA TAAGTTTGTG 
AACAGGTTTC ATGAAGAGAG GCGGACCAAG CTCAGCCTCC TCTTAGACAA CGAGCGTTGG 
AAGCAGGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATAGC GGATGGAAAG 
ATTGCTTTAC CTGAAAAAAA GCCAGTGGTT ACAGAAGAAA GGAAGCCAGC TGATGTTCTT 
GTTGTTGAGG GACACCAGTA TGCAGTTGTC GGAACCGTCT TGCTGTTAAT AAGAATCATT 
CTTGAATATT GCCAGTGTGT GGATAACATC CCCTCCGTCA CTACTGACAT GCTCACTCGC 
CTGACAGATC TGCTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCTTTGCAAG TTGTTGGACT AAAAACAATT ACTACAAAAA ACTTGGCCCT CTCTTCACGG 
TGTCTGCAGT TGATTGTGCA CTACATTCCC GTGATCCGGG CTCACTTTGA AGCCCGACTG 
CCCCCCAAGC AATGGAGCAT GCTTAGACAT TTTGATCACA TCACAAAGGA TTACCATGAT 
CATATAGCTG AAATTTCAGC TAAGCTTGTA GCGATAATGG ATAGCCTATT TGATAAGCTG 
TTATCAAAGT ATGAGGTGAA AGCTCCTGTT CCTTCCCCGT GTTTCAGGAA TATTTGTAAG 
CAAATGACAA AAATGCATGA AGCTATATTT GATCTTCTTC CCGAAGAACA AACACAGATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAACAGTT ATCACACTTA 
AATGTAATAA ATGACGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATCTGG ATCTAAATAT GGCCGAGATC 
TGGGAACAGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps54 ( XM_006514699.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514762.1 Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu45208
Clone ID Related Accession (Same CDS sequence)	XM_006514699.4 , XM_006514699.3
Accession Version	XM_006514699.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514699.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG ATTTTGCCTT GGATGATTCT TTAACTTTTA ATTCAGTTTT ACCATGGTCT 
CATTTTAATA CTGCTGGTGG GAAAGGAAGT CGCGACGCAG CTTCTTCCAA GTTGCTTCAG 
GAAAAGCTGA GCCATTATCT GGATATTGTG GAAGTAAACA TTGCTCACCA GATCTCTCTA 
CGTTCAGAAG CATTTTTTCA TGCAATGACC TCTCAACACG AATTGCAGGA CTACCTCAAG 
AAAACTACCC AGGCTGTAAA AATGCTTCGA GATAAAATTG CACAGATTGA TAAAGTTATG 
TGTGAAGGAT CACTACAGAT TTTAAGACTG GCACTTACCA GAAATAATTG TGTTAAAGTA 
TACAATAAAC TGAAGTTAAT GGCCACTGTA CACCAAACTC AGCCTACAGT GCAGGTGTTG 
TTATCTACTT CTGAATTTGT TGGAGCGTTG GACTTAATAG CAACAACTCA AGAGGTTCTG 
CAGCAGGAGC TTCAGGGCAT TCACAGCTTC CGGCATTTGG GATCACAGCT TTGTGAATTA 
GAAAAGCTGA TTGATAAAAT GATGATTGCA GAATTTTCTA CTTATTCTCA TAGTGACTTA 
AATAGACCAC TGGAAGGTGA ATGCCAAGTT TTAGAAGAGG AAAGATTAGT ATCTCTTGTA 
TTTGGGCTTT TGAAACAAAG AAAACTTAAT TTTTTAGAAA TCTATGGTGA AGAAATGATT 
ATTACAGCAA AGAACATCAT TAAAGAGCGT GTGATTAATA AAGTTTCACA AATAGAAGAA 
ATAGACACAG ACGTTGTTGT AAAGCTTGCA GATCAAATGA GAATGTTGAA TTTTCCCCAG 
TGGATTGATC TGTTAAAGGA CATTTTCTCT AAGTTTACAG TTTTCCTACA GAGAGTTAAG 
GCAACATTGA ATATTATCCA CAGTGTTGTT CTGTCAGTTC TTGAGAAAAG CCAAAGGACT 
AGAGAATTGG AAGAGATCCC ACAGCAGAGG AGCGCTGGGA AGGACAGCTC CTTGGACACA 
GACGTGGCTT ACCTGACACA CGAGGGCTGG TTCATAAGTG ATGCTTTCAG TGAGGGTGAG 
CCAGCATCTG CGGCGGTTGA TACTACCTCT CAAAGAAACA CGTCTCCACA TAGCGAGCCC 
TGCAGCAGTG ATTCGGTATC AGAACCAGAA TGTACTACTG ATTCTTCATC CAGCAAGGAG 
CAGACATCAG CGTGTGCTCC TCCAGGAGGT ATAGAGATTA TAGTCAGTGA AGACATGAGG 
TTAACTGACT TGGAGCTAGG AAAACTAGCA AGTAACATCC AGGAGTTACT GTGTAATGCT 
TCAGATGTAT GCCATGATCG AGCTGTCAAA TTTCTCATGT CAAGAGCAAA GGACGGTTTT 
CTTGAAAAAT TGAATTCCAC GGAATTCATA GCACTCTCGA GATTAATGGA GACATTCATA 
GTGGACACTG AACAGATCTG TGGGAGGAAG AGCACGTCAC TGCTTGGGGC CCTTCAGAGC 
CAAGCAAATA AGTTTGTGAA CAGGTTTCAT GAAGAGAGGC GGACCAAGCT CAGCCTCCTC 
TTAGACAACG AGCGTTGGAA GCAGGCTGAT GTTCCTGCAG AATTTCAGGA TCTTGTTGAT 
TCTATAGCGG ATGGAAAGAT TGCTTTACCT GAAAAAAAGC CAGTGGTTAC AGAAGAAAGG 
AAGCCAGCTG ATGTTCTTGT TGTTGAGGGA CACCAGTATG CAGTTGTCGG AACCGTCTTG 
CTGTTAATAA GAATCATTCT TGAATATTGC CAGTGTGTGG ATAACATCCC CTCCGTCACT 
ACTGACATGC TCACTCGCCT GACAGATCTG CTGAAGTACT TCAATTCAAG AAGTTGCCAG 
TTAGTTCTTG GAGCTGGTGC TTTGCAAGTT GTTGGACTAA AAACAATTAC TACAAAAAAC 
TTGGCCCTCT CTTCACGGTG TCTGCAGTTG ATTGTGCACT ACATTCCCGT GATCCGGGCT 
CACTTTGAAG CCCGACTGCC CCCCAAGCAA TGGAGCATGC TTAGACATTT TGATCACATC 
ACAAAGGATT ACCATGATCA TATAGCTGAA ATTTCAGCTA AGCTTGTAGC GATAATGGAT 
AGCCTATTTG ATAAGCTGTT ATCAAAGTAT GAGGTGAAAG CTCCTGTTCC TTCCCCGTGT 
TTCAGGAATA TTTGTAAGCA AATGACAAAA ATGCATGAAG CTATATTTGA TCTTCTTCCC 
GAAGAACAAA CACAGATGTT ATTTTTAAGA ATTAATGCAA GTTACAAACT CCACTTGAAA 
AAACAGTTAT CACACTTAAA TGTAATAAAT GACGGAGGAC CTCAAAATGG GTTGGTCACA 
GCAGATGTAG CTTTTTACAC TGGAAATCTT CAAGCCTTAA AAGGCCTTAA AGATCTGGAT 
CTAAATATGG CCGAGATCTG GGAACAGAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514762.1
CDS	64..2499
Translation	MKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVVVKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGKIALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_006514699.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS54 GARP complex subunit (Vps54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514699.4

ORF Insert Sequence:

ATGAAACCAG ATTTTGCCTT GGATGATTCT TTAACTTTTA ATTCAGTTTT ACCATGGTCT 
CATTTTAATA CTGCTGGTGG GAAAGGAAGT CGCGACGCAG CTTCTTCCAA GTTGCTTCAG 
GAAAAGCTGA GCCATTATCT GGATATTGTG GAAGTAAACA TTGCTCACCA GATCTCTCTA 
CGTTCAGAAG CATTTTTTCA TGCAATGACC TCTCAACACG AATTGCAGGA CTACCTCAAG 
AAAACTACCC AGGCTGTAAA AATGCTTCGA GATAAAATTG CACAGATTGA TAAAGTTATG 
TGTGAAGGAT CACTACAGAT TTTAAGACTG GCACTTACCA GAAATAATTG TGTTAAAGTA 
TACAATAAAC TGAAGTTAAT GGCCACTGTA CACCAAACTC AGCCTACAGT GCAGGTGTTG 
TTATCTACTT CTGAATTTGT TGGAGCGTTG GACTTAATAG CAACAACTCA AGAGGTTCTG 
CAGCAGGAGC TTCAGGGCAT TCACAGCTTC CGGCATTTGG GATCACAGCT TTGTGAATTA 
GAAAAGCTGA TTGATAAAAT GATGATTGCA GAATTTTCTA CTTATTCTCA TAGTGACTTA 
AATAGACCAC TGGAAGGTGA ATGCCAAGTT TTAGAAGAGG AAAGATTAGT ATCTCTTGTA 
TTTGGGCTTT TGAAACAAAG AAAACTTAAT TTTTTAGAAA TCTATGGTGA AGAAATGATT 
ATTACAGCAA AGAACATCAT TAAAGAGCGT GTGATTAATA AAGTTTCACA AATAGAAGAA 
ATAGACACAG ACGTTGTTGT AAAGCTTGCA GATCAAATGA GAATGTTGAA TTTTCCCCAG 
TGGATTGATC TGTTAAAGGA CATTTTCTCT AAGTTTACAG TTTTCCTACA GAGAGTTAAG 
GCAACATTGA ATATTATCCA CAGTGTTGTT CTGTCAGTTC TTGAGAAAAG CCAAAGGACT 
AGAGAATTGG AAGAGATCCC ACAGCAGAGG AGCGCTGGGA AGGACAGCTC CTTGGACACA 
GACGTGGCTT ACCTGACACA CGAGGGCTGG TTCATAAGTG ATGCTTTCAG TGAGGGTGAG 
CCAGCATCTG CGGCGGTTGA TACTACCTCT CAAAGAAACA CGTCTCCACA TAGCGAGCCC 
TGCAGCAGTG ATTCGGTATC AGAACCAGAA TGTACTACTG ATTCTTCATC CAGCAAGGAG 
CAGACATCAG CGTGTGCTCC TCCAGGAGGT ATAGAGATTA TAGTCAGTGA AGACATGAGG 
TTAACTGACT TGGAGCTAGG AAAACTAGCA AGTAACATCC AGGAGTTACT GTGTAATGCT 
TCAGATGTAT GCCATGATCG AGCTGTCAAA TTTCTCATGT CAAGAGCAAA GGACGGTTTT 
CTTGAAAAAT TGAATTCCAC GGAATTCATA GCACTCTCGA GATTAATGGA GACATTCATA 
GTGGACACTG AACAGATCTG TGGGAGGAAG AGCACGTCAC TGCTTGGGGC CCTTCAGAGC 
CAAGCAAATA AGTTTGTGAA CAGGTTTCAT GAAGAGAGGC GGACCAAGCT CAGCCTCCTC 
TTAGACAACG AGCGTTGGAA GCAGGCTGAT GTTCCTGCAG AATTTCAGGA TCTTGTTGAT 
TCTATAGCGG ATGGAAAGAT TGCTTTACCT GAAAAAAAGC CAGTGGTTAC AGAAGAAAGG 
AAGCCAGCTG ATGTTCTTGT TGTTGAGGGA CACCAGTATG CAGTTGTCGG AACCGTCTTG 
CTGTTAATAA GAATCATTCT TGAATATTGC CAGTGTGTGG ATAACATCCC CTCCGTCACT 
ACTGACATGC TCACTCGCCT GACAGATCTG CTGAAGTACT TCAATTCAAG AAGTTGCCAG 
TTAGTTCTTG GAGCTGGTGC TTTGCAAGTT GTTGGACTAA AAACAATTAC TACAAAAAAC 
TTGGCCCTCT CTTCACGGTG TCTGCAGTTG ATTGTGCACT ACATTCCCGT GATCCGGGCT 
CACTTTGAAG CCCGACTGCC CCCCAAGCAA TGGAGCATGC TTAGACATTT TGATCACATC 
ACAAAGGATT ACCATGATCA TATAGCTGAA ATTTCAGCTA AGCTTGTAGC GATAATGGAT 
AGCCTATTTG ATAAGCTGTT ATCAAAGTAT GAGGTGAAAG CTCCTGTTCC TTCCCCGTGT 
TTCAGGAATA TTTGTAAGCA AATGACAAAA ATGCATGAAG CTATATTTGA TCTTCTTCCC 
GAAGAACAAA CACAGATGTT ATTTTTAAGA ATTAATGCAA GTTACAAACT CCACTTGAAA 
AAACAGTTAT CACACTTAAA TGTAATAAAT GACGGAGGAC CTCAAAATGG GTTGGTCACA 
GCAGATGTAG CTTTTTACAC TGGAAATCTT CAAGCCTTAA AAGGCCTTAA AGATCTGGAT 
CTAAATATGG CCGAGATCTG GGAACAGAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.
Cell reports19(13)2823-2835(2017 Jun)
Wei J,Zhang YY,Luo J,Wang JQ,Zhou YX,Miao HH,Shi XJ,Qu YX,Xu J,Li BL,Song BL

Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant.
Cell and tissue research(2017 Mar)
Gioria M,Pasini ME,Berruti G

Steroid Profiling in Male Wobbler Mouse, a Model of Amyotrophic Lateral Sclerosis.
Endocrinology157(11)4446-4460(2016 Nov)
Gonzalez Deniselle MC,Liere P,Pianos A,Meyer M,Aprahamian F,Cambourg A,Di Giorgio NP,Schumacher M,De Nicola AF,Guennoun R

The Spatiotemporal Pattern of Degeneration in the Cerebellum of the Wobbler Mouse.
Journal of neuropathology and experimental neurology75(4)347-57(2016 Apr)
Saberi D,Ott B,Dahlke C,Matschke V,Schmitt-John T,Theiss C

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Vps54 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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