VPS54 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS54
Entrez Gene ID	783762
Full Name	VPS54, GARP complex subunit
General protein information	Preferred Names VPS54, GARP complex subunit Names vacuolar protein sorting-associated protein 54 vacuolar protein sorting 54 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005212810.3	XP_005212867.1	vacuolar protein sorting-associated protein 54 isoform X1
XM_015473490.1	XP_015328976.1	vacuolar protein sorting-associated protein 54 isoform X3
XM_005212810.4	XP_005212867.1	vacuolar protein sorting-associated protein 54 isoform X1
XM_005212812.4	XP_005212869.1	vacuolar protein sorting-associated protein 54 isoform X2
XM_024999247.1	XP_024855015.1	vacuolar protein sorting-associated protein 54 isoform X4
NM_001105460.2	NP_001098930.1	vacuolar protein sorting-associated protein 54
XM_005212812.3	XP_005212869.1	vacuolar protein sorting-associated protein 54 isoform X2
XM_015473490.2	XP_015328976.1	vacuolar protein sorting-associated protein 54 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VPS54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa24077	NM_001105460.2 Latest version!	Bos taurus VPS54 subunit of GARP complex (VPS54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa24077	XM_005212810.4 Latest version!	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa24078	XM_005212812.4 Latest version!	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa220694	XM_024999247.1 Latest version!	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa91894	XM_015473490.2 Latest version!	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa24077	XM_005212810.3	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa24078	XM_005212812.3	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa91894	XM_015473490.1	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS54 ( NM_001105460.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098930.1 Bos taurus VPS54 subunit of GARP complex (VPS54), mRNA.

Price & Availability↑

CloneID	OBa24077
Clone ID Related Accession (Same CDS sequence)	XM_005212810.3 , XM_005212810.4 , NM_001105460.2
Accession Version	NM_001105460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-29
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134641.1. On Aug 30, 2012 this sequence version replaced NM_001105460.1. ##Evidence-Data-START## Transcript exon combination :: BC134641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098930.1
CDS	24..2957
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSSSLCVAPSLVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	NM_001105460.2
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54 subunit of GARP complex (VPS54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105460.2

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_005212810.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212867.1 Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24077
Clone ID Related Accession (Same CDS sequence)	XM_005212810.3 , XM_005212810.4 , NM_001105460.2
Accession Version	XM_005212810.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104294.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212867.1
CDS	219..3152
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSSSLCVAPSLVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_005212810.3
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212810.3

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_005212810.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212867.1 Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24077
Clone ID Related Accession (Same CDS sequence)	XM_005212810.3 , XM_005212810.4 , NM_001105460.2
Accession Version	XM_005212810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212867.1
CDS	139..3072
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSSSLCVAPSLVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_005212810.4
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212810.4

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGCTCTTTCT TCACGCTGTT TGCAGTTAAT TGTGCACTAC 
ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTGCAAC CTAAGCAATA CAGTATGCTT 
AGGCATTTTG ATCATATCAC TAAGGACTAC CATGACCACA TAGCTGAAAT ATCAGCTAAG 
CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAAGCT 
CCCGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGGCAAAAAT GCATGAAGCT 
ATATTTGATC TCCTTCCTGA AGAACAAACG CAAATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT 
CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA 
GGCCTTCAAG ATTTGGACCT AAATATGGCC GAGATCTGGG AACAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_005212812.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212869.1 Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24078
Clone ID Related Accession (Same CDS sequence)	XM_005212812.4 , XM_005212812.3
Accession Version	XM_005212812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTA TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT GTATCAACAG GAAATCTCTC AGAGAGAAAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GAACTCTCTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGACGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTGC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTT TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTACAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAAGA ACTTCAGGGC 
ATTCATAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ATTGGAAGAT 
GACTGTCACA TTTTAGAAGA GGAAAGACTA ATCTCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAACTTA ATTTTTTAGA AATATATAGT GAAGAAATGA TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCG CAAATAGAAG ATATAGACAC AGATGTTGTT 
TTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCTC AGTGGTTTGA TTTACTAACG 
GATATTTTCT CCAAGTTTAC AATTTTCCTA CAGAGAGTTA AGGCAACCTT AAATATCATT 
CATAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AAAATGCTGC AAAAGATAAT TCACTAGACA CAGAAGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC AGTGAGAGTG AATTAACACC TATAGCCATA 
GATACTACCT CTCAGAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCAGATATC ATCATCTGTT 
ACTCCAGGAG CTGTGGATAT TATGGTCAAT GAAGATGTGA AATTAAGTGA CTTAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAGTTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCCGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCT 
ACAGAATTCA TATCACTTTC CAGATTAATG GAAACATTCA TTTTAGAAAC TGAACAGATA 
TGTGGAAGAA AAAGCATGTC ATTACTTGGA GCACTTCAGA GCCAAGCAAA TAAATTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAA CTCAGCCTTC TCTTAGACAA TGAACGCTGG 
AAGCAAGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATATC ACATGGAAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGGGCT ACAGAAGAAA GAAAACCAGC TGAAGTTCTT 
ACTGTTGAGG GGCAACAGTA TGCTGTTGTT GGAACTGTAT TGCTGTTGAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAATATC CCATCTGTTA CTACTGACAT GCTCACTCGT 
CTATCAGACT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACAATA ACTACAAAAA ATTTGGCTCT TTCTTCACGC 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTG 
CAACCTAAGC AATACAGTAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAC 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA AGCTCCCGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGGCAA AAATGCATGA AGCTATATTT GATCTCCTTC CTGAAGAACA AACGCAAATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT CAAGATTTGG ACCTAAATAT GGCCGAGATC 
TGGGAACAGA AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212869.1
CDS	142..3039
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_005212812.4
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212812.4

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTA TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT GTATCAACAG GAAATCTCTC AGAGAGAAAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GAACTCTCTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGACGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTGC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTT TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTACAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAAGA ACTTCAGGGC 
ATTCATAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ATTGGAAGAT 
GACTGTCACA TTTTAGAAGA GGAAAGACTA ATCTCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAACTTA ATTTTTTAGA AATATATAGT GAAGAAATGA TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCG CAAATAGAAG ATATAGACAC AGATGTTGTT 
TTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCTC AGTGGTTTGA TTTACTAACG 
GATATTTTCT CCAAGTTTAC AATTTTCCTA CAGAGAGTTA AGGCAACCTT AAATATCATT 
CATAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AAAATGCTGC AAAAGATAAT TCACTAGACA CAGAAGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC AGTGAGAGTG AATTAACACC TATAGCCATA 
GATACTACCT CTCAGAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCAGATATC ATCATCTGTT 
ACTCCAGGAG CTGTGGATAT TATGGTCAAT GAAGATGTGA AATTAAGTGA CTTAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAGTTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCCGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCT 
ACAGAATTCA TATCACTTTC CAGATTAATG GAAACATTCA TTTTAGAAAC TGAACAGATA 
TGTGGAAGAA AAAGCATGTC ATTACTTGGA GCACTTCAGA GCCAAGCAAA TAAATTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAA CTCAGCCTTC TCTTAGACAA TGAACGCTGG 
AAGCAAGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATATC ACATGGAAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGGGCT ACAGAAGAAA GAAAACCAGC TGAAGTTCTT 
ACTGTTGAGG GGCAACAGTA TGCTGTTGTT GGAACTGTAT TGCTGTTGAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAATATC CCATCTGTTA CTACTGACAT GCTCACTCGT 
CTATCAGACT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACAATA ACTACAAAAA ATTTGGCTCT TTCTTCACGC 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTG 
CAACCTAAGC AATACAGTAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAC 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA AGCTCCCGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGGCAA AAATGCATGA AGCTATATTT GATCTCCTTC CTGAAGAACA AACGCAAATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT CAAGATTTGG ACCTAAATAT GGCCGAGATC 
TGGGAACAGA AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_005212812.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212869.1 Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24078
Clone ID Related Accession (Same CDS sequence)	XM_005212812.4 , XM_005212812.3
Accession Version	XM_005212812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104294.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTA TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT GTATCAACAG GAAATCTCTC AGAGAGAAAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GAACTCTCTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGACGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTGC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTT TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTACAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAAGA ACTTCAGGGC 
ATTCATAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ATTGGAAGAT 
GACTGTCACA TTTTAGAAGA GGAAAGACTA ATCTCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAACTTA ATTTTTTAGA AATATATAGT GAAGAAATGA TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCG CAAATAGAAG ATATAGACAC AGATGTTGTT 
TTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCTC AGTGGTTTGA TTTACTAACG 
GATATTTTCT CCAAGTTTAC AATTTTCCTA CAGAGAGTTA AGGCAACCTT AAATATCATT 
CATAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AAAATGCTGC AAAAGATAAT TCACTAGACA CAGAAGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC AGTGAGAGTG AATTAACACC TATAGCCATA 
GATACTACCT CTCAGAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCAGATATC ATCATCTGTT 
ACTCCAGGAG CTGTGGATAT TATGGTCAAT GAAGATGTGA AATTAAGTGA CTTAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAGTTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCCGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCT 
ACAGAATTCA TATCACTTTC CAGATTAATG GAAACATTCA TTTTAGAAAC TGAACAGATA 
TGTGGAAGAA AAAGCATGTC ATTACTTGGA GCACTTCAGA GCCAAGCAAA TAAATTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAA CTCAGCCTTC TCTTAGACAA TGAACGCTGG 
AAGCAAGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATATC ACATGGAAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGGGCT ACAGAAGAAA GAAAACCAGC TGAAGTTCTT 
ACTGTTGAGG GGCAACAGTA TGCTGTTGTT GGAACTGTAT TGCTGTTGAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAATATC CCATCTGTTA CTACTGACAT GCTCACTCGT 
CTATCAGACT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACAATA ACTACAAAAA ATTTGGCTCT TTCTTCACGC 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTG 
CAACCTAAGC AATACAGTAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAC 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA AGCTCCCGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGGCAA AAATGCATGA AGCTATATTT GATCTCCTTC CTGAAGAACA AACGCAAATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT CAAGATTTGG ACCTAAATAT GGCCGAGATC 
TGGGAACAGA AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212869.1
CDS	219..3116
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_005212812.3
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212812.3

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTA TATATCATTC CAAAGTAAAT 
CTCCCAGCAG CATTAAATGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA 
ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC 
AGCAAGGAAC ATTTTACAGT GTATCAACAG GAAATCTCTC AGAGAGAAAA GATTCATGAG 
AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTTGACA GAACTCTCTT ACATACTCAT 
GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC 
TTGGACGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT 
GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTGC AAGAAAAGCT GAGCCATTAT 
CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTT TACGTTCAGA AGCATTTTTT 
CATGCAATGA CCTCTCAACA CGAATTGCAG GACTACCTCA AGAAAACTTC CCAGGCTGTA 
AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTACAC 
ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA ACTGAAGTTA 
ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TGTTATCTAC TTCTGAATTT 
GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAAGA ACTTCAGGGC 
ATTCATAGCT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA 
ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ATTGGAAGAT 
GACTGTCACA TTTTAGAAGA GGAAAGACTA ATCTCTCTTG TATTTGGACT TTTAAAACAA 
AGAAAACTTA ATTTTTTAGA AATATATAGT GAAGAAATGA TTATTACAGC AAAGAATATC 
ATTAAACAGT GTGTGATTAA TAAAGTTTCG CAAATAGAAG ATATAGACAC AGATGTTGTT 
TTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCTC AGTGGTTTGA TTTACTAACG 
GATATTTTCT CCAAGTTTAC AATTTTCCTA CAGAGAGTTA AGGCAACCTT AAATATCATT 
CATAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT 
TCACAACAGA AAAATGCTGC AAAAGATAAT TCACTAGACA CAGAAGTGGC TTATTTAATC 
CATGAAGGCA TGTTTATAAG TGATGCATTC AGTGAGAGTG AATTAACACC TATAGCCATA 
GATACTACCT CTCAGAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA 
TCCGAACCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCAGATATC ATCATCTGTT 
ACTCCAGGAG CTGTGGATAT TATGGTCAAT GAAGATGTGA AATTAAGTGA CTTAGAGCTA 
GGAAAGCTGG CAAATAATAT CCAGGAGTTA TTATATAGTG CCTCAGATAT ATGCCATGAT 
CGAGCCGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCT 
ACAGAATTCA TATCACTTTC CAGATTAATG GAAACATTCA TTTTAGAAAC TGAACAGATA 
TGTGGAAGAA AAAGCATGTC ATTACTTGGA GCACTTCAGA GCCAAGCAAA TAAATTTGTA 
AATAGGTTTC ATGAAGAGAG AAAAACCAAA CTCAGCCTTC TCTTAGACAA TGAACGCTGG 
AAGCAAGCTG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTATATC ACATGGAAAG 
ATTGCTTTAC CTGAAAAAAA ATCAGGGGCT ACAGAAGAAA GAAAACCAGC TGAAGTTCTT 
ACTGTTGAGG GGCAACAGTA TGCTGTTGTT GGAACTGTAT TGCTGTTGAT AAGAATTATC 
CTTGAATATT GCCAGTGTGT GGATAATATC CCATCTGTTA CTACTGACAT GCTCACTCGT 
CTATCAGACT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT 
GCACTGCAAG TTGTTGGACT AAAAACAATA ACTACAAAAA ATTTGGCTCT TTCTTCACGC 
TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTG 
CAACCTAAGC AATACAGTAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAC 
CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG 
TTATCTAAGT ATGAAGTGAA AGCTCCCGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG 
CAAATGGCAA AAATGCATGA AGCTATATTT GATCTCCTTC CTGAAGAACA AACGCAAATG 
TTATTTTTAA GAATTAATGC AAGTTACAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA 
AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC 
ACTGGAAATC TTCAAGCCTT AAAAGGCCTT CAAGATTTGG ACCTAAATAT GGCCGAGATC 
TGGGAACAGA AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_024999247.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855015.1 Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa220694
Clone ID Related Accession (Same CDS sequence)	XM_024999247.1
Accession Version	XM_024999247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855015.1
CDS	139..2565
Translation	MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTGDSSSLCVAPSLVTDQHRWTIYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLV

Target ORF information:

RefSeq Version	XM_024999247.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999247.1

ORF Insert Sequence:

ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
AAAAAAGAGG TAGACCCGAC AAAACACATT CGACCTGTGC AGTCACTGCC AGATGTGTGT 
CCCAAGGAAC CCACAGGTGA TTCAAGTAGT TTATGTGTTG CCCCATCTCT AGTTACAGAT 
CAACATAGAT GGACTATATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAATGATCCT 
AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC 
ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTGTAT 
CAACAGGAAA TCTCTCAGAG AGAAAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT 
AAAGATACCT TTGACAGAAC TCTCTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ACGATTCCTT AACTTTTAAT 
TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT 
TCCTCAAAGT TGCTGCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT 
GCTCACCAGA TCTCTTTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAA 
TTGCAGGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA 
CAGATTGATA AAGTAATGTG TGAAGGATCA CTACACATTT TAAGACTGGC ACTTACCAGA 
AATAATTGTG TTAAAGTATA CAATAAACTG AAGTTAATGG CCACTGTACA CCAGACTCAG 
CCTACAGTAC AGGTGTTGTT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA 
ACAACACAAG AGGTTCTACA GCAAGAACTT CAGGGCATTC ATAGCTTCCG GCATTTGGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT 
TATTCTCACA GTGACTTAAA TAGACCATTG GAAGATGACT GTCACATTTT AGAAGAGGAA 
AGACTAATCT CTCTTGTATT TGGACTTTTA AAACAAAGAA AACTTAATTT TTTAGAAATA 
TATAGTGAAG AAATGATTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA 
GTTTCGCAAA TAGAAGATAT AGACACAGAT GTTGTTTTGA AGCTTGCAGA TCAGATGAGA 
ATGTTGAATT TTCCTCAGTG GTTTGATTTA CTAACGGATA TTTTCTCCAA GTTTACAATT 
TTCCTACAGA GAGTTAAGGC AACCTTAAAT ATCATTCATA GTGTTGTTCT CTCAGTTCTT 
GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAAAA TGCTGCAAAA 
GATAATTCAC TAGACACAGA AGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT 
GCATTCAGTG AGAGTGAATT AACACCTATA GCCATAGATA CTACCTCTCA GAGAAATGCA 
TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AACCAGAATG TACTACTGAT 
TCTTCATCCA GCAAAGAGCA GATATCATCA TCTGTTACTC CAGGAGCTGT GGATATTATG 
GTCAATGAAG ATGTGAAATT AAGTGACTTA GAGCTAGGAA AGCTGGCAAA TAATATCCAG 
GAGTTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CCGTCAAATT TCTCATGTCA 
AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCTACAG AATTCATATC ACTTTCCAGA 
TTAATGGAAA CATTCATTTT AGAAACTGAA CAGATATGTG GAAGAAAAAG CATGTCATTA 
CTTGGAGCAC TTCAGAGCCA AGCAAATAAA TTTGTAAATA GGTTTCATGA AGAGAGAAAA 
ACCAAACTCA GCCTTCTCTT AGACAATGAA CGCTGGAAGC AAGCTGATGT TCCTGCAGAA 
TTTCAGGATC TTGTTGATTC TATATCACAT GGAAAGATTG CTTTACCTGA AAAAAAATCA 
GGGGCTACAG AAGAAAGAAA ACCAGCTGAA GTTCTTACTG TTGAGGGGCA ACAGTATGCT 
GTTGTTGGAA CTGTATTGCT GTTGATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT 
AATATCCCAT CTGTTACTAC TGACATGCTC ACTCGTCTAT CAGACTTATT GAAGTACTTC 
AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA 
ACAATAACTA CAAAAAATTT GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_015473490.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328976.1 Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa91894
Clone ID Related Accession (Same CDS sequence)	XM_015473490.1 , XM_015473490.2
Accession Version	XM_015473490.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104294.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCC CAAGGAACCC ACAGATCAGC AAGGAACATT TTACAGTGTA TCAACAGGAA 
ATCTCTCAGA GAGAAAAGAT TCATGAGAGA TGCAAGAATA TTTGTCCTCC TAAAGATACC 
TTTGACAGAA CTCTCTTACA TACTCATGAT AAATCCAGGA CAGATCTGGA GCAAGTACCT 
AAGATTTTTA TGAAACCAGA TTTTGCCTTG GACGATTCCT TAACTTTTAA TTCAGTTTTA 
CCATGGTCTC ATTTTAATAC TGCTGGTGGA AAAGGAAATC GTGATGCAGC TTCCTCAAAG 
TTGCTGCAAG AAAAGCTGAG CCATTATCTG GATATTGTGG AAGTAAACAT TGCTCACCAG 
ATCTCTTTAC GTTCAGAAGC ATTTTTTCAT GCAATGACCT CTCAACACGA ATTGCAGGAC 
TACCTCAAGA AAACTTCCCA GGCTGTAAAA ATGCTTCGAG ATAAAATTGC ACAGATTGAT 
AAAGTAATGT GTGAAGGATC ACTACACATT TTAAGACTGG CACTTACCAG AAATAATTGT 
GTTAAAGTAT ACAATAAACT GAAGTTAATG GCCACTGTAC ACCAGACTCA GCCTACAGTA 
CAGGTGTTGT TATCTACTTC TGAATTTGTT GGAGCATTGG ACTTAATAGC AACAACACAA 
GAGGTTCTAC AGCAAGAACT TCAGGGCATT CATAGCTTCC GGCATTTGGG ATCACAGCTT 
TGTGAATTAG AAAAACTGAT AGATAAAATG ATGATTGCAG AATTTTCTAC TTATTCTCAC 
AGTGACTTAA ATAGACCATT GGAAGATGAC TGTCACATTT TAGAAGAGGA AAGACTAATC 
TCTCTTGTAT TTGGACTTTT AAAACAAAGA AAACTTAATT TTTTAGAAAT ATATAGTGAA 
GAAATGATTA TTACAGCAAA GAATATCATT AAACAGTGTG TGATTAATAA AGTTTCGCAA 
ATAGAAGATA TAGACACAGA TGTTGTTTTG AAGCTTGCAG ATCAGATGAG AATGTTGAAT 
TTTCCTCAGT GGTTTGATTT ACTAACGGAT ATTTTCTCCA AGTTTACAAT TTTCCTACAG 
AGAGTTAAGG CAACCTTAAA TATCATTCAT AGTGTTGTTC TCTCAGTTCT TGACAAAAAC 
CAAAGGACTA GAGAATTGGA AGAGATTTCA CAACAGAAAA ATGCTGCAAA AGATAATTCA 
CTAGACACAG AAGTGGCTTA TTTAATCCAT GAAGGCATGT TTATAAGTGA TGCATTCAGT 
GAGAGTGAAT TAACACCTAT AGCCATAGAT ACTACCTCTC AGAGAAATGC ATCTCCAAAT 
AGTGAGCCCT GCAGCAGTGA TTCTGTATCC GAACCAGAAT GTACTACTGA TTCTTCATCC 
AGCAAAGAGC AGATATCATC ATCTGTTACT CCAGGAGCTG TGGATATTAT GGTCAATGAA 
GATGTGAAAT TAAGTGACTT AGAGCTAGGA AAGCTGGCAA ATAATATCCA GGAGTTATTA 
TATAGTGCCT CAGATATATG CCATGATCGA GCCGTCAAAT TTCTCATGTC AAGAGCAAAG 
GATGGTTTTC TTGAGAAGCT AAATTCTACA GAATTCATAT CACTTTCCAG ATTAATGGAA 
ACATTCATTT TAGAAACTGA ACAGATATGT GGAAGAAAAA GCATGTCATT ACTTGGAGCA 
CTTCAGAGCC AAGCAAATAA ATTTGTAAAT AGGTTTCATG AAGAGAGAAA AACCAAACTC 
AGCCTTCTCT TAGACAATGA ACGCTGGAAG CAAGCTGATG TTCCTGCAGA ATTTCAGGAT 
CTTGTTGATT CTATATCACA TGGAAAGATT GCTTTACCTG AAAAAAAATC AGGGGCTACA 
GAAGAAAGAA AACCAGCTGA AGTTCTTACT GTTGAGGGGC AACAGTATGC TGTTGTTGGA 
ACTGTATTGC TGTTGATAAG AATTATCCTT GAATATTGCC AGTGTGTGGA TAATATCCCA 
TCTGTTACTA CTGACATGCT CACTCGTCTA TCAGACTTAT TGAAGTACTT CAATTCAAGA 
AGTTGCCAGT TAGTTCTTGG AGCTGGTGCA CTGCAAGTTG TTGGACTAAA AACAATAACT 
ACAAAAAATT TGGCTCTTTC TTCACGCTGT TTGCAGTTAA TTGTGCACTA CATTCCTGTG 
ATCCGGGCTC ATTTTGAAGC TCGACTGCAA CCTAAGCAAT ACAGTATGCT TAGGCATTTT 
GATCATATCA CTAAGGACTA CCATGACCAC ATAGCTGAAA TATCAGCTAA GCTTGTAGCG 
ATAATGGATA GCTTATTTGA CAAGCTGTTA TCTAAGTATG AAGTGAAAGC TCCCGTTCCT 
TCTGCCTGTT TCAGGAATAT TTGTAAGCAA ATGGCAAAAA TGCATGAAGC TATATTTGAT 
CTCCTTCCTG AAGAACAAAC GCAAATGTTA TTTTTAAGAA TTAATGCAAG TTACAAACTC 
CACTTGAAAA AGCAGTTATC TCACTTAAAT GTGATAAATG ATGGAGGACC TCAAAATGGG 
TTGGTCACAG CAGATGTAGC TTTTTACACT GGAAATCTTC AAGCCTTAAA AGGCCTTCAA 
GATTTGGACC TAAATATGGC CGAGATCTGG GAACAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328976.1
CDS	110..2734
Translation	MCVPRNPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_015473490.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54 GARP complex subunit (VPS54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473490.1

ORF Insert Sequence:

ATGTGTGTCC CAAGGAACCC ACAGATCAGC AAGGAACATT TTACAGTGTA TCAACAGGAA 
ATCTCTCAGA GAGAAAAGAT TCATGAGAGA TGCAAGAATA TTTGTCCTCC TAAAGATACC 
TTTGACAGAA CTCTCTTACA TACTCATGAT AAATCCAGGA CAGATCTGGA GCAAGTACCT 
AAGATTTTTA TGAAACCAGA TTTTGCCTTG GACGATTCCT TAACTTTTAA TTCAGTTTTA 
CCATGGTCTC ATTTTAATAC TGCTGGTGGA AAAGGAAATC GTGATGCAGC TTCCTCAAAG 
TTGCTGCAAG AAAAGCTGAG CCATTATCTG GATATTGTGG AAGTAAACAT TGCTCACCAG 
ATCTCTTTAC GTTCAGAAGC ATTTTTTCAT GCAATGACCT CTCAACACGA ATTGCAGGAC 
TACCTCAAGA AAACTTCCCA GGCTGTAAAA ATGCTTCGAG ATAAAATTGC ACAGATTGAT 
AAAGTAATGT GTGAAGGATC ACTACACATT TTAAGACTGG CACTTACCAG AAATAATTGT 
GTTAAAGTAT ACAATAAACT GAAGTTAATG GCCACTGTAC ACCAGACTCA GCCTACAGTA 
CAGGTGTTGT TATCTACTTC TGAATTTGTT GGAGCATTGG ACTTAATAGC AACAACACAA 
GAGGTTCTAC AGCAAGAACT TCAGGGCATT CATAGCTTCC GGCATTTGGG ATCACAGCTT 
TGTGAATTAG AAAAACTGAT AGATAAAATG ATGATTGCAG AATTTTCTAC TTATTCTCAC 
AGTGACTTAA ATAGACCATT GGAAGATGAC TGTCACATTT TAGAAGAGGA AAGACTAATC 
TCTCTTGTAT TTGGACTTTT AAAACAAAGA AAACTTAATT TTTTAGAAAT ATATAGTGAA 
GAAATGATTA TTACAGCAAA GAATATCATT AAACAGTGTG TGATTAATAA AGTTTCGCAA 
ATAGAAGATA TAGACACAGA TGTTGTTTTG AAGCTTGCAG ATCAGATGAG AATGTTGAAT 
TTTCCTCAGT GGTTTGATTT ACTAACGGAT ATTTTCTCCA AGTTTACAAT TTTCCTACAG 
AGAGTTAAGG CAACCTTAAA TATCATTCAT AGTGTTGTTC TCTCAGTTCT TGACAAAAAC 
CAAAGGACTA GAGAATTGGA AGAGATTTCA CAACAGAAAA ATGCTGCAAA AGATAATTCA 
CTAGACACAG AAGTGGCTTA TTTAATCCAT GAAGGCATGT TTATAAGTGA TGCATTCAGT 
GAGAGTGAAT TAACACCTAT AGCCATAGAT ACTACCTCTC AGAGAAATGC ATCTCCAAAT 
AGTGAGCCCT GCAGCAGTGA TTCTGTATCC GAACCAGAAT GTACTACTGA TTCTTCATCC 
AGCAAAGAGC AGATATCATC ATCTGTTACT CCAGGAGCTG TGGATATTAT GGTCAATGAA 
GATGTGAAAT TAAGTGACTT AGAGCTAGGA AAGCTGGCAA ATAATATCCA GGAGTTATTA 
TATAGTGCCT CAGATATATG CCATGATCGA GCCGTCAAAT TTCTCATGTC AAGAGCAAAG 
GATGGTTTTC TTGAGAAGCT AAATTCTACA GAATTCATAT CACTTTCCAG ATTAATGGAA 
ACATTCATTT TAGAAACTGA ACAGATATGT GGAAGAAAAA GCATGTCATT ACTTGGAGCA 
CTTCAGAGCC AAGCAAATAA ATTTGTAAAT AGGTTTCATG AAGAGAGAAA AACCAAACTC 
AGCCTTCTCT TAGACAATGA ACGCTGGAAG CAAGCTGATG TTCCTGCAGA ATTTCAGGAT 
CTTGTTGATT CTATATCACA TGGAAAGATT GCTTTACCTG AAAAAAAATC AGGGGCTACA 
GAAGAAAGAA AACCAGCTGA AGTTCTTACT GTTGAGGGGC AACAGTATGC TGTTGTTGGA 
ACTGTATTGC TGTTGATAAG AATTATCCTT GAATATTGCC AGTGTGTGGA TAATATCCCA 
TCTGTTACTA CTGACATGCT CACTCGTCTA TCAGACTTAT TGAAGTACTT CAATTCAAGA 
AGTTGCCAGT TAGTTCTTGG AGCTGGTGCA CTGCAAGTTG TTGGACTAAA AACAATAACT 
ACAAAAAATT TGGCTCTTTC TTCACGCTGT TTGCAGTTAA TTGTGCACTA CATTCCTGTG 
ATCCGGGCTC ATTTTGAAGC TCGACTGCAA CCTAAGCAAT ACAGTATGCT TAGGCATTTT 
GATCATATCA CTAAGGACTA CCATGACCAC ATAGCTGAAA TATCAGCTAA GCTTGTAGCG 
ATAATGGATA GCTTATTTGA CAAGCTGTTA TCTAAGTATG AAGTGAAAGC TCCCGTTCCT 
TCTGCCTGTT TCAGGAATAT TTGTAAGCAA ATGGCAAAAA TGCATGAAGC TATATTTGAT 
CTCCTTCCTG AAGAACAAAC GCAAATGTTA TTTTTAAGAA TTAATGCAAG TTACAAACTC 
CACTTGAAAA AGCAGTTATC TCACTTAAAT GTGATAAATG ATGGAGGACC TCAAAATGGG 
TTGGTCACAG CAGATGTAGC TTTTTACACT GGAAATCTTC AAGCCTTAAA AGGCCTTCAA 
GATTTGGACC TAAATATGGC CGAGATCTGG GAACAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_015473490.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328976.1 Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa91894
Clone ID Related Accession (Same CDS sequence)	XM_015473490.1 , XM_015473490.2
Accession Version	XM_015473490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCC CAAGGAACCC ACAGATCAGC AAGGAACATT TTACAGTGTA TCAACAGGAA 
ATCTCTCAGA GAGAAAAGAT TCATGAGAGA TGCAAGAATA TTTGTCCTCC TAAAGATACC 
TTTGACAGAA CTCTCTTACA TACTCATGAT AAATCCAGGA CAGATCTGGA GCAAGTACCT 
AAGATTTTTA TGAAACCAGA TTTTGCCTTG GACGATTCCT TAACTTTTAA TTCAGTTTTA 
CCATGGTCTC ATTTTAATAC TGCTGGTGGA AAAGGAAATC GTGATGCAGC TTCCTCAAAG 
TTGCTGCAAG AAAAGCTGAG CCATTATCTG GATATTGTGG AAGTAAACAT TGCTCACCAG 
ATCTCTTTAC GTTCAGAAGC ATTTTTTCAT GCAATGACCT CTCAACACGA ATTGCAGGAC 
TACCTCAAGA AAACTTCCCA GGCTGTAAAA ATGCTTCGAG ATAAAATTGC ACAGATTGAT 
AAAGTAATGT GTGAAGGATC ACTACACATT TTAAGACTGG CACTTACCAG AAATAATTGT 
GTTAAAGTAT ACAATAAACT GAAGTTAATG GCCACTGTAC ACCAGACTCA GCCTACAGTA 
CAGGTGTTGT TATCTACTTC TGAATTTGTT GGAGCATTGG ACTTAATAGC AACAACACAA 
GAGGTTCTAC AGCAAGAACT TCAGGGCATT CATAGCTTCC GGCATTTGGG ATCACAGCTT 
TGTGAATTAG AAAAACTGAT AGATAAAATG ATGATTGCAG AATTTTCTAC TTATTCTCAC 
AGTGACTTAA ATAGACCATT GGAAGATGAC TGTCACATTT TAGAAGAGGA AAGACTAATC 
TCTCTTGTAT TTGGACTTTT AAAACAAAGA AAACTTAATT TTTTAGAAAT ATATAGTGAA 
GAAATGATTA TTACAGCAAA GAATATCATT AAACAGTGTG TGATTAATAA AGTTTCGCAA 
ATAGAAGATA TAGACACAGA TGTTGTTTTG AAGCTTGCAG ATCAGATGAG AATGTTGAAT 
TTTCCTCAGT GGTTTGATTT ACTAACGGAT ATTTTCTCCA AGTTTACAAT TTTCCTACAG 
AGAGTTAAGG CAACCTTAAA TATCATTCAT AGTGTTGTTC TCTCAGTTCT TGACAAAAAC 
CAAAGGACTA GAGAATTGGA AGAGATTTCA CAACAGAAAA ATGCTGCAAA AGATAATTCA 
CTAGACACAG AAGTGGCTTA TTTAATCCAT GAAGGCATGT TTATAAGTGA TGCATTCAGT 
GAGAGTGAAT TAACACCTAT AGCCATAGAT ACTACCTCTC AGAGAAATGC ATCTCCAAAT 
AGTGAGCCCT GCAGCAGTGA TTCTGTATCC GAACCAGAAT GTACTACTGA TTCTTCATCC 
AGCAAAGAGC AGATATCATC ATCTGTTACT CCAGGAGCTG TGGATATTAT GGTCAATGAA 
GATGTGAAAT TAAGTGACTT AGAGCTAGGA AAGCTGGCAA ATAATATCCA GGAGTTATTA 
TATAGTGCCT CAGATATATG CCATGATCGA GCCGTCAAAT TTCTCATGTC AAGAGCAAAG 
GATGGTTTTC TTGAGAAGCT AAATTCTACA GAATTCATAT CACTTTCCAG ATTAATGGAA 
ACATTCATTT TAGAAACTGA ACAGATATGT GGAAGAAAAA GCATGTCATT ACTTGGAGCA 
CTTCAGAGCC AAGCAAATAA ATTTGTAAAT AGGTTTCATG AAGAGAGAAA AACCAAACTC 
AGCCTTCTCT TAGACAATGA ACGCTGGAAG CAAGCTGATG TTCCTGCAGA ATTTCAGGAT 
CTTGTTGATT CTATATCACA TGGAAAGATT GCTTTACCTG AAAAAAAATC AGGGGCTACA 
GAAGAAAGAA AACCAGCTGA AGTTCTTACT GTTGAGGGGC AACAGTATGC TGTTGTTGGA 
ACTGTATTGC TGTTGATAAG AATTATCCTT GAATATTGCC AGTGTGTGGA TAATATCCCA 
TCTGTTACTA CTGACATGCT CACTCGTCTA TCAGACTTAT TGAAGTACTT CAATTCAAGA 
AGTTGCCAGT TAGTTCTTGG AGCTGGTGCA CTGCAAGTTG TTGGACTAAA AACAATAACT 
ACAAAAAATT TGGCTCTTTC TTCACGCTGT TTGCAGTTAA TTGTGCACTA CATTCCTGTG 
ATCCGGGCTC ATTTTGAAGC TCGACTGCAA CCTAAGCAAT ACAGTATGCT TAGGCATTTT 
GATCATATCA CTAAGGACTA CCATGACCAC ATAGCTGAAA TATCAGCTAA GCTTGTAGCG 
ATAATGGATA GCTTATTTGA CAAGCTGTTA TCTAAGTATG AAGTGAAAGC TCCCGTTCCT 
TCTGCCTGTT TCAGGAATAT TTGTAAGCAA ATGGCAAAAA TGCATGAAGC TATATTTGAT 
CTCCTTCCTG AAGAACAAAC GCAAATGTTA TTTTTAAGAA TTAATGCAAG TTACAAACTC 
CACTTGAAAA AGCAGTTATC TCACTTAAAT GTGATAAATG ATGGAGGACC TCAAAATGGG 
TTGGTCACAG CAGATGTAGC TTTTTACACT GGAAATCTTC AAGCCTTAAA AGGCCTTCAA 
GATTTGGACC TAAATATGGC CGAGATCTGG GAACAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328976.1
CDS	131..2755
Translation	MCVPRNPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCHILEEERLISLVFGLLKQRKLNFLEIYSEEMIITAKNIIKQCVINKVSQIEDIDTDVVLKLADQMRMLNFPQWFDLLTDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFSESELTPIAIDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEQISSSVTPGAVDIMVNEDVKLSDLELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSTEFISLSRLMETFILETEQICGRKSMSLLGALQSQANKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISHGKIALPEKKSGATEERKPAEVLTVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLQPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMAKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLQDLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_015473490.2
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS54, GARP complex subunit (VPS54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473490.2

ORF Insert Sequence:

ATGTGTGTCC CAAGGAACCC ACAGATCAGC AAGGAACATT TTACAGTGTA TCAACAGGAA 
ATCTCTCAGA GAGAAAAGAT TCATGAGAGA TGCAAGAATA TTTGTCCTCC TAAAGATACC 
TTTGACAGAA CTCTCTTACA TACTCATGAT AAATCCAGGA CAGATCTGGA GCAAGTACCT 
AAGATTTTTA TGAAACCAGA TTTTGCCTTG GACGATTCCT TAACTTTTAA TTCAGTTTTA 
CCATGGTCTC ATTTTAATAC TGCTGGTGGA AAAGGAAATC GTGATGCAGC TTCCTCAAAG 
TTGCTGCAAG AAAAGCTGAG CCATTATCTG GATATTGTGG AAGTAAACAT TGCTCACCAG 
ATCTCTTTAC GTTCAGAAGC ATTTTTTCAT GCAATGACCT CTCAACACGA ATTGCAGGAC 
TACCTCAAGA AAACTTCCCA GGCTGTAAAA ATGCTTCGAG ATAAAATTGC ACAGATTGAT 
AAAGTAATGT GTGAAGGATC ACTACACATT TTAAGACTGG CACTTACCAG AAATAATTGT 
GTTAAAGTAT ACAATAAACT GAAGTTAATG GCCACTGTAC ACCAGACTCA GCCTACAGTA 
CAGGTGTTGT TATCTACTTC TGAATTTGTT GGAGCATTGG ACTTAATAGC AACAACACAA 
GAGGTTCTAC AGCAAGAACT TCAGGGCATT CATAGCTTCC GGCATTTGGG ATCACAGCTT 
TGTGAATTAG AAAAACTGAT AGATAAAATG ATGATTGCAG AATTTTCTAC TTATTCTCAC 
AGTGACTTAA ATAGACCATT GGAAGATGAC TGTCACATTT TAGAAGAGGA AAGACTAATC 
TCTCTTGTAT TTGGACTTTT AAAACAAAGA AAACTTAATT TTTTAGAAAT ATATAGTGAA 
GAAATGATTA TTACAGCAAA GAATATCATT AAACAGTGTG TGATTAATAA AGTTTCGCAA 
ATAGAAGATA TAGACACAGA TGTTGTTTTG AAGCTTGCAG ATCAGATGAG AATGTTGAAT 
TTTCCTCAGT GGTTTGATTT ACTAACGGAT ATTTTCTCCA AGTTTACAAT TTTCCTACAG 
AGAGTTAAGG CAACCTTAAA TATCATTCAT AGTGTTGTTC TCTCAGTTCT TGACAAAAAC 
CAAAGGACTA GAGAATTGGA AGAGATTTCA CAACAGAAAA ATGCTGCAAA AGATAATTCA 
CTAGACACAG AAGTGGCTTA TTTAATCCAT GAAGGCATGT TTATAAGTGA TGCATTCAGT 
GAGAGTGAAT TAACACCTAT AGCCATAGAT ACTACCTCTC AGAGAAATGC ATCTCCAAAT 
AGTGAGCCCT GCAGCAGTGA TTCTGTATCC GAACCAGAAT GTACTACTGA TTCTTCATCC 
AGCAAAGAGC AGATATCATC ATCTGTTACT CCAGGAGCTG TGGATATTAT GGTCAATGAA 
GATGTGAAAT TAAGTGACTT AGAGCTAGGA AAGCTGGCAA ATAATATCCA GGAGTTATTA 
TATAGTGCCT CAGATATATG CCATGATCGA GCCGTCAAAT TTCTCATGTC AAGAGCAAAG 
GATGGTTTTC TTGAGAAGCT AAATTCTACA GAATTCATAT CACTTTCCAG ATTAATGGAA 
ACATTCATTT TAGAAACTGA ACAGATATGT GGAAGAAAAA GCATGTCATT ACTTGGAGCA 
CTTCAGAGCC AAGCAAATAA ATTTGTAAAT AGGTTTCATG AAGAGAGAAA AACCAAACTC 
AGCCTTCTCT TAGACAATGA ACGCTGGAAG CAAGCTGATG TTCCTGCAGA ATTTCAGGAT 
CTTGTTGATT CTATATCACA TGGAAAGATT GCTTTACCTG AAAAAAAATC AGGGGCTACA 
GAAGAAAGAA AACCAGCTGA AGTTCTTACT GTTGAGGGGC AACAGTATGC TGTTGTTGGA 
ACTGTATTGC TGTTGATAAG AATTATCCTT GAATATTGCC AGTGTGTGGA TAATATCCCA 
TCTGTTACTA CTGACATGCT CACTCGTCTA TCAGACTTAT TGAAGTACTT CAATTCAAGA 
AGTTGCCAGT TAGTTCTTGG AGCTGGTGCA CTGCAAGTTG TTGGACTAAA AACAATAACT 
ACAAAAAATT TGGCTCTTTC TTCACGCTGT TTGCAGTTAA TTGTGCACTA CATTCCTGTG 
ATCCGGGCTC ATTTTGAAGC TCGACTGCAA CCTAAGCAAT ACAGTATGCT TAGGCATTTT 
GATCATATCA CTAAGGACTA CCATGACCAC ATAGCTGAAA TATCAGCTAA GCTTGTAGCG 
ATAATGGATA GCTTATTTGA CAAGCTGTTA TCTAAGTATG AAGTGAAAGC TCCCGTTCCT 
TCTGCCTGTT TCAGGAATAT TTGTAAGCAA ATGGCAAAAA TGCATGAAGC TATATTTGAT 
CTCCTTCCTG AAGAACAAAC GCAAATGTTA TTTTTAAGAA TTAATGCAAG TTACAAACTC 
CACTTGAAAA AGCAGTTATC TCACTTAAAT GTGATAAATG ATGGAGGACC TCAAAATGGG 
TTGGTCACAG CAGATGTAGC TTTTTACACT GGAAATCTTC AAGCCTTAAA AGGCCTTCAA 
GATTTGGACC TAAATATGGC CGAGATCTGG GAACAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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VPS54 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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