TJP1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc69378 XM_009428687.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69379 XM_009428688.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69380 XM_016927839.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69381 XM_016927840.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69383 XM_001163120.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69384 XM_016927841.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69385 XM_016927842.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69386 XM_001163045.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc69387 XM_016927843.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OPc97826 XM_009428684.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97827 XM_009428685.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97828 XM_009428686.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97829 XM_009428689.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97830 XM_009428687.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97831 XM_009428688.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97832 XM_024349006.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97833 XM_009428690.3
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97834 XM_024349007.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97835 XM_024349008.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97836 XM_001163120.4
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97837 XM_016927841.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97838 XM_016927842.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97839 XM_024349009.1
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97840 XM_001163045.4
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc97841 XM_016927843.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OPc69374 XM_009428684.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OPc69375 XM_009428685.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OPc69376 XM_009428686.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OPc69377 XM_009428689.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OPc69382 XM_009428690.2
Latest version!
Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc69378
    Clone ID Related Accession (Same CDS sequence) XM_009428687.2
    Accession Version XM_009428687.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426962.2
    CDS110..5428
    Translation

    Target ORF information:

    RefSeq Version XM_009428687.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428687.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69379
    Clone ID Related Accession (Same CDS sequence) XM_009428688.2
    Accession Version XM_009428688.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426963.2
    CDS470..5776
    Translation

    Target ORF information:

    RefSeq Version XM_009428688.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69380
    Clone ID Related Accession (Same CDS sequence) XM_016927839.1
    Accession Version XM_016927839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783328.1
    CDS16..5316
    Translation

    Target ORF information:

    RefSeq Version XM_016927839.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69381
    Clone ID Related Accession (Same CDS sequence) XM_016927840.1
    Accession Version XM_016927840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTGCC ACAGTAAGAG CACAGCAATG GAGGAAACAG CTATATGGGA ACAACATACA 
    GTGACGCTTC ACAGGGCTCC TGGATTTGGG TTTGGAATTG CAATATCTGG TGGACGAGAT
    AATCCTCATT TTCAGAGTGG GGAAACGTCA ATAGTGATTT CAGATGTGCT GAAAGGAGGA
    CCAGCTGAAG GACAGCTACA GGAAAATGAC CGAGTTGCAA TGGTTAACGG AGTTTCAATG
    GATAATGTTG AACATGCTTT TGCTGTTCAG CAACTAAGGA AAAGTGGGAA AAATGCAAAA
    ATTACAATTA GAAGGAAGAA GAAAGTTCAA ATACCAGTAA GTCGTCCTGA TCCTGAACCA
    GTATCTGATA ATGAAGAAGA TAGTTATGAT GAGGAAATAC ATGATCCAAG AAGTGGCCGG
    AGTGGTGTGG TTAACAGAAG GAGTGAGAAG ATTTGGCCGA GGGATAGAAG TGCAAGTAGA
    GAGAGGAGCT TGTCCCCGCG GTCAGACAGG CGGTCAGTGG CTTCCAGCCA GCCCGCTAAA
    CCTACTAAAG TCACACTGGT GAAATCCCGG AAAAATGAAG AATATGGTCT TCGATTGGCA
    AGCCATATAT TTGTTAAGGA AATTTCACAA GATAGTTTGG CAGCAAGAGA TGGCAATATT
    CAAGAAGGTG ATGTTGTATT GAAGATAAAT GGTACTGTGA CAGAAAATAT GTCATTGACA
    GATGCAAAGA CATTGATAGA AAGGTCTAAA GGCAAATTAA AAATGGTAGT TCAAAGAGAT
    GAACGGGCTA CGCTATTGAA TGTCCCTGAT CTTTCTGACA GCATCCACTC TGCTAATGCC
    TCTGAGAGAG ACGACATTTC AGAAATTCAG TCACTGGCAT CAGATCATTC TGGTCGATCA
    CACGATAGGC CTCCCCGCCG CAGCCGGTCA CGATCTCCTG ACCAGCGGTC AGAGCCTTCT
    GATCATTCCA GGCACTCGCC ACAGCAGCCA AGCAATGGCA GTCTCCGGAG TAGAGATGAA
    GAGAGAATTT CTAAACCTGG GGCTGTCTCA ACTCCTGTAA AGCATGCTGA TGATCACACA
    CCTAAAACAG TGGAAGAAGT TACAGTTGAA AGAAATGAGA AACAAACACC TTCTCTTCCA
    GAACCAAAGC CTGTGTATGC CCAAGTTGGG CAGCCAGATG TGGATTTACC TGTCAGTCCA
    TCTGATGGTG TCCTACCTAA TTCAACTCAT GAAGATGGGA TTCTTCGGCC CAGCATGAAA
    TTGGTAAAAT TCAGAAAAGG AGATAGTGTG GGTTTGCGGC TGGCTGGTGG AAATGATGTT
    GGAATATTTG TAGCTGGCGT TCTAGAAGAT AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA
    GGTGATCAAA TTCTCAGGGT AAACAACGTA GATTTTACAA ATATCATAAG AGAAGAAGCC
    GTCCTTTTCC TGCTTGACCT CCCTAAAGGA GAAGAAGTGA CCATATTGGC TCAGAAGAAG
    AAGGATGTTT ATCGTCGCAT TGTAGAATCA GATGTAGGAG ATTCTTTCTA TATTAGAACC
    CATTTTGAAT ATGAAAAGGA ATCTCCCTAT GGACTTAGTT TTAACAAAGG AGAGGTGTTC
    CGTGTTGTGG ATACCTTGTA CAATGGAAAA CTGGGCTCTT GGCTTGCTAT TCGAATTGGT
    AAAAATCATA AGGAGGTAGA ACGAGGCATC ATCCCTAATA AGAACAGAGC TGAGCAGCTA
    GCCAGTGTAC AGTATACACT TCCAAAAACA GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA
    TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT
    CAGCCTGTTC AAACAAAGTT TCCAGCTTAT GAAAGAGTGG TTCTTCGAGA AGCTGGATTT
    CTGAGGCCTG TAACCATTTT TGGACCAATA GCTGATGTTG CCAGAGAAAA GCTGGCAAGA
    GAAGAACCAG ATATTTATCA AATTGCAAAG AGTGAACCAC GAGACGCTGG AACTGACCAA
    CGTAGCTCTG GCATTATTCG CCTGCATACA ATAAAGCAAA TCATAGATCA AGACAAACAT
    GCTTTATTAG ATGTAACACC AAATGCAGTT GATCGTCTTA ACTATGCCCA GTGGTATCCA
    ATTGTTGTAT TTCTTAACCC TGATTCTAAG CAAGGAGTAA AAACAATGAG AATGAGGTTA
    TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG TTATATGAGC GATCTCATAA ACTTCGTAAA
    AATAATCACC ATCTTTTTAC AACTACAATT AACTTAAATT CAATGAATGA TGGTTGGTAT
    GGTGCGCTGA AAGAAGCAAT TCAACAACAG CAAAACCAGC TGGTATGGGT TTCCGAGGGA
    AAGGCGGATG GTGCTACAAG TGATGACCTT GATTTGCATG ATGATCGTCT GTCCTACCTG
    TCAGCTCCAG GTAGTGAATA CTCAATGTAT AGCACGGACA GTAGACACAC TTCTGACTAT
    GAAGACACAG ACACAGAAGG CGGGGCCTAC ACTGATCAAG AACTAGATGA AACTCTTAAT
    GATGAGGTTG GGACTCCACC GGAGTCTGCC ATTACACGGT CCTCTGAGCC TGTAAGAGAG
    GACTCCTCTG GAATGCATCA TGAAAACCAA ACATATCCTC CTTACTCACC ACAAGCGCAG
    CCACAACCAA TTCATAGAAT AGACTCCCCT GGATTTAAGC CAGCCTCTCA ACAGAAAGCA
    GAAGCTTCAT CTCCAGTCCC TTACCTTTCG CCTGAAACAA ACCCAGCATC ATCAACCTCT
    GCTGTTAATC ATAATGTAAA TTTAACTAAT GTCAGACTGG AGGAGCCCAC CCCAGCTCCT
    TCCACCTCTT ACTCACCACA AGCTGATTCT TTAAGAACAC CAAGTACTGA GGCAGCTCAC
    ATAATGCTAA GAGATCAAGA ACCATCATTG TCGTCGCATG TAGATCCAAC AAAGGTGTAT
    AGAAAGGATC CATATCCCGA GGAAATGATG AGGCAGAACC ATGTTTTGAA ACAGCCAGCC
    GTTAGTCACC CAGGGCACAG GCCAGACAAA GAGCCTAATC TGACCTATGA ACCCCAACTC
    CCATACGTAG AGAAACAAGC CAGCAGAGAC CTCGAGCAGC CCACATACAG ATACGAGTCC
    TCAAGCTATA CGGACCAGTT TTCTCGAAAC TATGAACATC GTCTGCGATA CGAAGATCGC
    GTCCCCATGT ATGAAGAACA GTGGTCATAT TATGATGACA AACAGCCCTA CCCATCTCGG
    CCACCTTTTG ATAATCAGCA CTCTCAAGAC CTTGACTCCA GACAGCATCC CGAAGAGTCC
    TCAGAACGAG GGTACTTTCC ACGTTTTGAA GAGCCAGCCC CTCTGTCTTA CGACAGCAGA
    CCACGTTACG AACAGGCACC TAGAGCATCC GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT
    GGGTATGACA CACATGGTAG ACTCAGACCG GAAGCCCAGC CCCACCCTTC AGCAGGGCCC
    AAGCCCGCAG AGTCCAAGCA GTATTTTGAG CAATATTCAC GCAGTTACGA GCAAGTACCA
    CCCCAAGGAT TTACCTCTAG AGCAGGTCAT TTTGAGCCTC TCCATGGTGC TGCAGCTGTC
    CCTCCGCTGA TACCTTCATC TCAGCATAAG CCAGAAGCTC TGCCTTCAAA CACCAAACCA
    CTGCCTCCAC CCCCAACTCA AACCGAAGAA GAGGAAGATC CAGCAATGAA GCCACAGTCT
    GTACTCACCA GAGTTAAGAT GTTTGAAAAC AAAAGATCTG CATCCTTAGA GACCAAGAAG
    GATGTAAATG ACACTGGCAG TTTTAAGCCT CCAGAAGTAG CATCTAAACC TTCAGGTGCT
    CCCATCATTG GTCCCAAACC CACTTCTCAG AATCAGTTCA GTGAACATGA CAAAACTCTG
    TACAGGATCC CAGAACCTCA AAAACCTCAA CTGAAGCCAC CTGAAGATAT CGTTCGGTCC
    AATCATTATG ACCCTGAAGA AGATGAAGAA TATTATCGAA AACAGCTGTC ATACTTTGAC
    CGAAGAAGTT TTGAGAATAA GCCTCCTGCA CACATTGCCG CCAGCCATCT CTCCGAGCCT
    GCAAAGCCAG CTCATTCTCA GAATCAATCA AATTTTTCTA GTTATTCTTC AAAGGGAAAG
    CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG
    GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC
    AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG
    GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA
    ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGCCAG CTTTCTATCC CCAGAAAAGT
    TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA
    TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT
    GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT
    CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA
    ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG
    GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT
    GGGGGCGTGC TGAGTTCCAT AGAAACTGGC GTTAGTATAA TTATCCCTCA AGGAGCCATT
    CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA
    CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT
    GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783329.1
    CDS16..5313
    Translation

    Target ORF information:

    RefSeq Version XM_016927840.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTGCC ACAGTAAGAG CACAGCAATG GAGGAAACAG CTATATGGGA ACAACATACA 
    GTGACGCTTC ACAGGGCTCC TGGATTTGGG TTTGGAATTG CAATATCTGG TGGACGAGAT
    AATCCTCATT TTCAGAGTGG GGAAACGTCA ATAGTGATTT CAGATGTGCT GAAAGGAGGA
    CCAGCTGAAG GACAGCTACA GGAAAATGAC CGAGTTGCAA TGGTTAACGG AGTTTCAATG
    GATAATGTTG AACATGCTTT TGCTGTTCAG CAACTAAGGA AAAGTGGGAA AAATGCAAAA
    ATTACAATTA GAAGGAAGAA GAAAGTTCAA ATACCAGTAA GTCGTCCTGA TCCTGAACCA
    GTATCTGATA ATGAAGAAGA TAGTTATGAT GAGGAAATAC ATGATCCAAG AAGTGGCCGG
    AGTGGTGTGG TTAACAGAAG GAGTGAGAAG ATTTGGCCGA GGGATAGAAG TGCAAGTAGA
    GAGAGGAGCT TGTCCCCGCG GTCAGACAGG CGGTCAGTGG CTTCCAGCCA GCCCGCTAAA
    CCTACTAAAG TCACACTGGT GAAATCCCGG AAAAATGAAG AATATGGTCT TCGATTGGCA
    AGCCATATAT TTGTTAAGGA AATTTCACAA GATAGTTTGG CAGCAAGAGA TGGCAATATT
    CAAGAAGGTG ATGTTGTATT GAAGATAAAT GGTACTGTGA CAGAAAATAT GTCATTGACA
    GATGCAAAGA CATTGATAGA AAGGTCTAAA GGCAAATTAA AAATGGTAGT TCAAAGAGAT
    GAACGGGCTA CGCTATTGAA TGTCCCTGAT CTTTCTGACA GCATCCACTC TGCTAATGCC
    TCTGAGAGAG ACGACATTTC AGAAATTCAG TCACTGGCAT CAGATCATTC TGGTCGATCA
    CACGATAGGC CTCCCCGCCG CAGCCGGTCA CGATCTCCTG ACCAGCGGTC AGAGCCTTCT
    GATCATTCCA GGCACTCGCC ACAGCAGCCA AGCAATGGCA GTCTCCGGAG TAGAGATGAA
    GAGAGAATTT CTAAACCTGG GGCTGTCTCA ACTCCTGTAA AGCATGCTGA TGATCACACA
    CCTAAAACAG TGGAAGAAGT TACAGTTGAA AGAAATGAGA AACAAACACC TTCTCTTCCA
    GAACCAAAGC CTGTGTATGC CCAAGTTGGG CAGCCAGATG TGGATTTACC TGTCAGTCCA
    TCTGATGGTG TCCTACCTAA TTCAACTCAT GAAGATGGGA TTCTTCGGCC CAGCATGAAA
    TTGGTAAAAT TCAGAAAAGG AGATAGTGTG GGTTTGCGGC TGGCTGGTGG AAATGATGTT
    GGAATATTTG TAGCTGGCGT TCTAGAAGAT AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA
    GGTGATCAAA TTCTCAGGGT AAACAACGTA GATTTTACAA ATATCATAAG AGAAGAAGCC
    GTCCTTTTCC TGCTTGACCT CCCTAAAGGA GAAGAAGTGA CCATATTGGC TCAGAAGAAG
    AAGGATGTTT ATCGTCGCAT TGTAGAATCA GATGTAGGAG ATTCTTTCTA TATTAGAACC
    CATTTTGAAT ATGAAAAGGA ATCTCCCTAT GGACTTAGTT TTAACAAAGG AGAGGTGTTC
    CGTGTTGTGG ATACCTTGTA CAATGGAAAA CTGGGCTCTT GGCTTGCTAT TCGAATTGGT
    AAAAATCATA AGGAGGTAGA ACGAGGCATC ATCCCTAATA AGAACAGAGC TGAGCAGCTA
    GCCAGTGTAC AGTATACACT TCCAAAAACA GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA
    TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT
    CAGCCTGTTC AAACAAAGTT TCCAGCTTAT GAAAGAGTGG TTCTTCGAGA AGCTGGATTT
    CTGAGGCCTG TAACCATTTT TGGACCAATA GCTGATGTTG CCAGAGAAAA GCTGGCAAGA
    GAAGAACCAG ATATTTATCA AATTGCAAAG AGTGAACCAC GAGACGCTGG AACTGACCAA
    CGTAGCTCTG GCATTATTCG CCTGCATACA ATAAAGCAAA TCATAGATCA AGACAAACAT
    GCTTTATTAG ATGTAACACC AAATGCAGTT GATCGTCTTA ACTATGCCCA GTGGTATCCA
    ATTGTTGTAT TTCTTAACCC TGATTCTAAG CAAGGAGTAA AAACAATGAG AATGAGGTTA
    TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG TTATATGAGC GATCTCATAA ACTTCGTAAA
    AATAATCACC ATCTTTTTAC AACTACAATT AACTTAAATT CAATGAATGA TGGTTGGTAT
    GGTGCGCTGA AAGAAGCAAT TCAACAACAG CAAAACCAGC TGGTATGGGT TTCCGAGGGA
    AAGGCGGATG GTGCTACAAG TGATGACCTT GATTTGCATG ATGATCGTCT GTCCTACCTG
    TCAGCTCCAG GTAGTGAATA CTCAATGTAT AGCACGGACA GTAGACACAC TTCTGACTAT
    GAAGACACAG ACACAGAAGG CGGGGCCTAC ACTGATCAAG AACTAGATGA AACTCTTAAT
    GATGAGGTTG GGACTCCACC GGAGTCTGCC ATTACACGGT CCTCTGAGCC TGTAAGAGAG
    GACTCCTCTG GAATGCATCA TGAAAACCAA ACATATCCTC CTTACTCACC ACAAGCGCAG
    CCACAACCAA TTCATAGAAT AGACTCCCCT GGATTTAAGC CAGCCTCTCA ACAGAAAGCA
    GAAGCTTCAT CTCCAGTCCC TTACCTTTCG CCTGAAACAA ACCCAGCATC ATCAACCTCT
    GCTGTTAATC ATAATGTAAA TTTAACTAAT GTCAGACTGG AGGAGCCCAC CCCAGCTCCT
    TCCACCTCTT ACTCACCACA AGCTGATTCT TTAAGAACAC CAAGTACTGA GGCAGCTCAC
    ATAATGCTAA GAGATCAAGA ACCATCATTG TCGTCGCATG TAGATCCAAC AAAGGTGTAT
    AGAAAGGATC CATATCCCGA GGAAATGATG AGGCAGAACC ATGTTTTGAA ACAGCCAGCC
    GTTAGTCACC CAGGGCACAG GCCAGACAAA GAGCCTAATC TGACCTATGA ACCCCAACTC
    CCATACGTAG AGAAACAAGC CAGCAGAGAC CTCGAGCAGC CCACATACAG ATACGAGTCC
    TCAAGCTATA CGGACCAGTT TTCTCGAAAC TATGAACATC GTCTGCGATA CGAAGATCGC
    GTCCCCATGT ATGAAGAACA GTGGTCATAT TATGATGACA AACAGCCCTA CCCATCTCGG
    CCACCTTTTG ATAATCAGCA CTCTCAAGAC CTTGACTCCA GACAGCATCC CGAAGAGTCC
    TCAGAACGAG GGTACTTTCC ACGTTTTGAA GAGCCAGCCC CTCTGTCTTA CGACAGCAGA
    CCACGTTACG AACAGGCACC TAGAGCATCC GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT
    GGGTATGACA CACATGGTAG ACTCAGACCG GAAGCCCAGC CCCACCCTTC AGCAGGGCCC
    AAGCCCGCAG AGTCCAAGCA GTATTTTGAG CAATATTCAC GCAGTTACGA GCAAGTACCA
    CCCCAAGGAT TTACCTCTAG AGCAGGTCAT TTTGAGCCTC TCCATGGTGC TGCAGCTGTC
    CCTCCGCTGA TACCTTCATC TCAGCATAAG CCAGAAGCTC TGCCTTCAAA CACCAAACCA
    CTGCCTCCAC CCCCAACTCA AACCGAAGAA GAGGAAGATC CAGCAATGAA GCCACAGTCT
    GTACTCACCA GAGTTAAGAT GTTTGAAAAC AAAAGATCTG CATCCTTAGA GACCAAGAAG
    GATGTAAATG ACACTGGCAG TTTTAAGCCT CCAGAAGTAG CATCTAAACC TTCAGGTGCT
    CCCATCATTG GTCCCAAACC CACTTCTCAG AATCAGTTCA GTGAACATGA CAAAACTCTG
    TACAGGATCC CAGAACCTCA AAAACCTCAA CTGAAGCCAC CTGAAGATAT CGTTCGGTCC
    AATCATTATG ACCCTGAAGA AGATGAAGAA TATTATCGAA AACAGCTGTC ATACTTTGAC
    CGAAGAAGTT TTGAGAATAA GCCTCCTGCA CACATTGCCG CCAGCCATCT CTCCGAGCCT
    GCAAAGCCAG CTCATTCTCA GAATCAATCA AATTTTTCTA GTTATTCTTC AAAGGGAAAG
    CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG
    GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC
    AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG
    GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA
    ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGCCAG CTTTCTATCC CCAGAAAAGT
    TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA
    TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT
    GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT
    CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA
    ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG
    GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT
    GGGGGCGTGC TGAGTTCCAT AGAAACTGGC GTTAGTATAA TTATCCCTCA AGGAGCCATT
    CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA
    CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT
    GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69383
    Clone ID Related Accession (Same CDS sequence) XM_001163120.3
    Accession Version XM_001163120.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001163120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001163120.2
    CDS476..5722
    Translation

    Target ORF information:

    RefSeq Version XM_001163120.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001163120.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69384
    Clone ID Related Accession (Same CDS sequence) XM_016927841.1
    Accession Version XM_016927841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783330.1
    CDS419..5719
    Translation

    Target ORF information:

    RefSeq Version XM_016927841.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69385
    Clone ID Related Accession (Same CDS sequence) XM_016927842.1
    Accession Version XM_016927842.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783331.1
    CDS110..5188
    Translation

    Target ORF information:

    RefSeq Version XM_016927842.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69386
    Clone ID Related Accession (Same CDS sequence) XM_001163045.3
    Accession Version XM_001163045.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001163045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001163045.2
    CDS476..5482
    Translation

    Target ORF information:

    RefSeq Version XM_001163045.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001163045.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69387
    Clone ID Related Accession (Same CDS sequence) XM_016927843.1
    Accession Version XM_016927843.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
    GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG
    AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT
    TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC
    CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG
    CCACAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT
    GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA
    GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT
    GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT
    AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA
    GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC
    GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG
    GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC
    CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC
    ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG
    GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG
    TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA
    GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA
    CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC
    TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG
    TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT
    TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT
    CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT
    CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA
    CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC
    CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA
    AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT
    ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA
    ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA
    AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA
    TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA
    GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA
    CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT
    CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA
    ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC
    CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA
    AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA
    CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA
    GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC
    GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC
    AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA
    GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG
    TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA
    CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG
    CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT
    CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA
    CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT
    AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG
    CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT
    AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA
    TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT
    CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG
    ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC
    AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA
    CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT
    CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA
    GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT
    AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT
    CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT
    GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT
    CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA
    CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA
    GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC
    CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA
    GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA
    AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT
    CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT
    TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA
    GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA
    GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT
    CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC
    ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG
    GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA
    GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG
    CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783332.1
    CDS89..4708
    Translation

    Target ORF information:

    RefSeq Version XM_016927843.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
    GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG
    AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT
    TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC
    CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG
    CCACAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT
    GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA
    GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT
    GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT
    AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA
    GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC
    GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG
    GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC
    CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC
    ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG
    GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG
    TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA
    GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA
    CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC
    TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG
    TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT
    TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT
    CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT
    CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA
    CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC
    CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA
    AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT
    ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA
    ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA
    AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA
    TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA
    GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA
    CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT
    CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA
    ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC
    CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA
    AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA
    CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA
    GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC
    GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC
    AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA
    GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG
    TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA
    CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG
    CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT
    CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA
    CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT
    AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG
    CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT
    AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA
    TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT
    CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG
    ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC
    AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA
    CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT
    CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA
    GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT
    AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT
    CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT
    GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT
    CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA
    CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA
    GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC
    CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA
    GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA
    AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT
    CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT
    TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA
    GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA
    GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT
    CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC
    ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG
    GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA
    GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG
    CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97826
    Clone ID Related Accession (Same CDS sequence) XM_009428684.3
    Accession Version XM_009428684.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426959.2
    CDS86..5671
    Translation

    Target ORF information:

    RefSeq Version XM_009428684.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428684.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97827
    Clone ID Related Accession (Same CDS sequence) XM_009428685.3
    Accession Version XM_009428685.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426960.2
    CDS17..5563
    Translation

    Target ORF information:

    RefSeq Version XM_009428685.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428685.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97828
    Clone ID Related Accession (Same CDS sequence) XM_009428686.3
    Accession Version XM_009428686.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426961.2
    CDS86..5611
    Translation

    Target ORF information:

    RefSeq Version XM_009428686.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428686.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97829
    Clone ID Related Accession (Same CDS sequence) XM_009428689.3
    Accession Version XM_009428689.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426964.2
    CDS16..5361
    Translation

    Target ORF information:

    RefSeq Version XM_009428689.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428689.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97830
    Clone ID Related Accession (Same CDS sequence) XM_009428687.3
    Accession Version XM_009428687.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426962.2
    CDS110..5428
    Translation

    Target ORF information:

    RefSeq Version XM_009428687.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97831
    Clone ID Related Accession (Same CDS sequence) XM_009428688.3
    Accession Version XM_009428688.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426963.2
    CDS476..5782
    Translation

    Target ORF information:

    RefSeq Version XM_009428688.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428688.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97832
    Clone ID Related Accession (Same CDS sequence) XM_024349006.1
    Accession Version XM_024349006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024204774.1
    CDS16..5322
    Translation

    Target ORF information:

    RefSeq Version XM_024349006.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024349006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97833
    Clone ID Related Accession (Same CDS sequence) XM_009428690.3
    Accession Version XM_009428690.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426965.2
    CDS16..5301
    Translation

    Target ORF information:

    RefSeq Version XM_009428690.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428690.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97834
    Clone ID Related Accession (Same CDS sequence) XM_024349007.1
    Accession Version XM_024349007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024204775.1
    CDS110..5389
    Translation

    Target ORF information:

    RefSeq Version XM_024349007.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024349007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97835
    Clone ID Related Accession (Same CDS sequence) XM_024349008.1
    Accession Version XM_024349008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024204776.1
    CDS476..5743
    Translation

    Target ORF information:

    RefSeq Version XM_024349008.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024349008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97836
    Clone ID Related Accession (Same CDS sequence) XM_001163120.4
    Accession Version XM_001163120.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001163120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001163120.2
    CDS476..5722
    Translation

    Target ORF information:

    RefSeq Version XM_001163120.4
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001163120.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97837
    Clone ID Related Accession (Same CDS sequence) XM_016927841.2
    Accession Version XM_016927841.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783330.1
    CDS419..5719
    Translation

    Target ORF information:

    RefSeq Version XM_016927841.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927841.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA
    AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC
    CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC
    ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA
    TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA
    GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA
    AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA
    GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG
    TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA
    TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG
    CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT
    CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA
    GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC
    AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC
    ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT
    CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC
    CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT
    CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC
    TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT
    GTCCTTATTG ACCACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97838
    Clone ID Related Accession (Same CDS sequence) XM_016927842.2
    Accession Version XM_016927842.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783331.1
    CDS110..5188
    Translation

    Target ORF information:

    RefSeq Version XM_016927842.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97839
    Clone ID Related Accession (Same CDS sequence) XM_024349009.1
    Accession Version XM_024349009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024204777.1
    CDS476..5542
    Translation

    Target ORF information:

    RefSeq Version XM_024349009.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024349009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97840
    Clone ID Related Accession (Same CDS sequence) XM_001163045.4
    Accession Version XM_001163045.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001163045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001163045.2
    CDS476..5482
    Translation

    Target ORF information:

    RefSeq Version XM_001163045.4
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001163045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc97841
    Clone ID Related Accession (Same CDS sequence) XM_016927843.2
    Accession Version XM_016927843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
    GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG
    AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT
    TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC
    CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG
    CCGCAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT
    GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA
    GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT
    GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT
    AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA
    GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC
    GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG
    GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC
    CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC
    ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG
    GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG
    TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA
    GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA
    CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC
    TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG
    TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT
    TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT
    CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT
    CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA
    CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC
    CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA
    AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT
    ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA
    ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA
    AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA
    TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA
    GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA
    CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT
    CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA
    ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC
    CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA
    AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA
    CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA
    GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC
    GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC
    AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA
    GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG
    TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA
    CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG
    CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT
    CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA
    CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT
    AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG
    CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT
    AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA
    TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT
    CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG
    ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC
    AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA
    CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT
    CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA
    GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT
    AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT
    CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT
    GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT
    CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA
    CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA
    GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC
    CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA
    GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA
    AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT
    CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT
    TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA
    GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA
    GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT
    CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC
    ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG
    GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA
    GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG
    CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016783332.1
    CDS89..4708
    Translation

    Target ORF information:

    RefSeq Version XM_016927843.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016927843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
    GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG
    AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT
    TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC
    CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG
    CCGCAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT
    GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA
    GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT
    GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT
    AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA
    GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC
    GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG
    GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC
    CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC
    ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG
    GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG
    TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA
    GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA
    CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC
    TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG
    TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT
    TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT
    CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT
    CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA
    CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC
    CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA
    AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT
    ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA
    ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA
    AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA
    TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA
    GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA
    CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT
    CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA
    ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC
    CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA
    AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA
    CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA
    GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC
    GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC
    AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA
    GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG
    TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA
    CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG
    CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT
    CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA
    CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT
    AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG
    CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT
    AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA
    TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT
    CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG
    ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC
    AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA
    CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT
    CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA
    GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT
    AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT
    CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT
    GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT
    CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA
    CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA
    GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC
    CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA
    GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA
    AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT
    CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT
    TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA
    GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA
    GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT
    CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC
    ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG
    GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA
    GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG
    CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69374
    Clone ID Related Accession (Same CDS sequence) XM_009428684.2
    Accession Version XM_009428684.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426959.2
    CDS18..5603
    Translation

    Target ORF information:

    RefSeq Version XM_009428684.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428684.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69375
    Clone ID Related Accession (Same CDS sequence) XM_009428685.2
    Accession Version XM_009428685.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426960.2
    CDS19..5565
    Translation

    Target ORF information:

    RefSeq Version XM_009428685.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428685.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69376
    Clone ID Related Accession (Same CDS sequence) XM_009428686.2
    Accession Version XM_009428686.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426961.2
    CDS19..5544
    Translation

    Target ORF information:

    RefSeq Version XM_009428686.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428686.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69377
    Clone ID Related Accession (Same CDS sequence) XM_009428689.2
    Accession Version XM_009428689.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426964.2
    CDS17..5362
    Translation

    Target ORF information:

    RefSeq Version XM_009428689.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc69382
    Clone ID Related Accession (Same CDS sequence) XM_009428690.2
    Accession Version XM_009428690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009426965.2
    CDS18..5303
    Translation

    Target ORF information:

    RefSeq Version XM_009428690.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009428690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.