Tjp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Tjp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tjp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa56459 XM_017588930.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa56460 XM_017588931.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa56461 XM_017588932.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa56462 XM_017588933.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa56463 XM_017588934.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa13314 NM_001106266.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa22874 XM_006229330.3
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa22875 XM_006229331.2
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa22875 XM_006229333.2
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa22875 XM_017588936.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa22876 XM_006229332.3
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa56464 XM_017588935.1
Latest version!
Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa56459
    Clone ID Related Accession (Same CDS sequence) XM_017588930.1
    Accession Version XM_017588930.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444419.1
    CDS6..5969
    Translation

    Target ORF information:

    RefSeq Version XM_017588930.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa56460
    Clone ID Related Accession (Same CDS sequence) XM_017588931.1
    Accession Version XM_017588931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTGACCCTAA AACTTGGCAA AACAAGTGTC TTCCTGGAGA TCCGAATTAC
    CTTGTTGGAG CCAACTGTGT TTCTGTCCTG ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444420.1
    CDS6..5930
    Translation

    Target ORF information:

    RefSeq Version XM_017588931.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTGACCCTAA AACTTGGCAA AACAAGTGTC TTCCTGGAGA TCCGAATTAC
    CTTGTTGGAG CCAACTGTGT TTCTGTCCTG ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa56461
    Clone ID Related Accession (Same CDS sequence) XM_017588932.1
    Accession Version XM_017588932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444421.1
    CDS6..5909
    Translation

    Target ORF information:

    RefSeq Version XM_017588932.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GTCCCTTACC TTTCGCCTGA AACAAACCCA
    GCATCATCAG CCTCTGCAGT TAAGCATAAT GTAAATTTAA CTAATGTCAA CCTGGAGGAG
    CCCACCCCAG CTCCTCCCAC CTCGCACGTA TCACAAGCTG ATTGTTTAGG AGCACCAAGT
    CCTGAGGCAC CTCACACGAT GCTCAGAGAC GAAGGAGTGT CATTGCCGTC GCATGTAGAC
    CCAGCAAAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa56462
    Clone ID Related Accession (Same CDS sequence) XM_017588933.1
    Accession Version XM_017588933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444422.1
    CDS6..5729
    Translation

    Target ORF information:

    RefSeq Version XM_017588933.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGCGTCCATG
    ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa56463
    Clone ID Related Accession (Same CDS sequence) XM_017588934.1
    Accession Version XM_017588934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444423.1
    CDS6..5669
    Translation

    Target ORF information:

    RefSeq Version XM_017588934.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCTAGTT GTGTAGATCC GGCCAGCAAG GCATCAGAGC AGCAGCCACC GGGAGGGAGC 
    CCTGTCTCCA GCTCCGTAAG GCATAGCATG TTGGAGCGAG GAGCTCACTC TTGGTTTTAT
    TCCTCTGAAA CAAGGACACA GTGGGAGAAG GAGAAGAGGA GTCAGTGGCT TGCAGAAATA
    CTGACGAGGT ACAAGGAGCG GATGCTGAAG GACTTCCACC TGGCCCGGGT GCTTCCCTAC
    TTGGTGTATG ATGGTGTGTT CTCCCTAAAA GAGTACAGGG AGATCCTCTC TCGGGATCGC
    TATCCAGAAA GAGTGGAGCA CTTTTTCCTG ACACTCTTCT CCAAAGGTCC GGGTGCTTTC
    TGTGCCTTCT GTGGTCATCT GGAGGAGCTC AGCCCTTACC TGCTCATCTC CCTTTTCCTT
    TATTACCAAG AGCAAACCCA AAGGATCCTA CAAGACGTGC TTGTGGCAGA AGAGGCAGAG
    AGGAGGAACA GAGCACAATG GGACTCTAGC ACTGTCTCTG ACCCAGAAGA CCAAGAACAA
    AGGGCTCGAT TGTTTAACAA GGTAACTCCA TCCTCTGAAA ACCCCCATGG TGCTACTTCC
    AATGTCAGCC AAGGGAAACC TTCTCTAAGA CGAATTAAAG GGAGACTACA TCGAAGCAAA
    AGCCTTGACA GTATCGACTT CTGTGAGCTC ACTAGCACAG CAATGGAGGA AACAGCTATA
    TGGGAACAGC ACACAGTGAC GCTTCACAGG GCTCCTGGGT TTGGATTTGG AATTGCAATA
    TCTGGTGGAA GAGATAATCC TCATTTTCAG AGTGGGGAAA CCTCCATAGT GATTTCTGAT
    GTGCTAAAAG GAGGGCCAGC TGAAGGACAG CTACAGGAAA ATGACCGAGT CGCAATGGTT
    AACGGAGTTT CAATGGATAA TGTTGAACAT GCTTTTGCTG TTCAGCAGCT AAGGAAAAGT
    GGGAAAAACG CAAAAATTAC CATCCGAAGA AAGAAGAAAG TTCAGATTCC TGTAAGTCAC
    CCAGACCCTG ACCCAGTGTC TGATAATGAA GATGATAGCT ATGACGAGGA TGTGCACGAT
    CCAAGAAGTG GCCGAGGTGC CCTAGCTAAC AGAAGGGGTG AGAAGAGCTG GGCAAGGGAT
    AGAAGCGCAA GCAGGGACCG GAGCCTGTCC CCTCGCTCAG ACAGGCGATC AGTGGCCTCC
    AGTCAGCCCG CCAAACCCAC CAAGGTCACA TTGGTGAAGT CTCGGAAAAA TGAAGAATAT
    GGTCTTCGAT TGGCCAGCCA TATATTTGTA AAGGAAATTT CACAAGATAG TTTGGCAGCA
    AGAGATGGTA ACATTCAAGA AGGGGATGTT GTCCTGAAGA TAAATGGTAC TGTGACAGAA
    AATATGTCAC TGACAGATGC AAAAACATTG ATAGAAAGGT CTAAAGGCAA GTTAAAAATG
    GTAGTGCAAA GAGATGAGCG GGCTACCTTA TTGAATGTCC CTGATCTTTC AGATAGTATC
    CATTCTGCTA ATGCCTCTGA AAGAGATGAC ATTTCAGAAA TTCAGTCACT AGCATCAGAC
    CATTCAGTTC GCTCCCATGA CAGGCCTCCC CGCCGAAGCC AGTCACGATC TCCCGACCAA
    CGTTCAGAGC CCTCTGATCA TTCCACACAG TCTCCACAGC AGCCCAGCAA TGGCAGTCTC
    CGGAGCAGAG AGGAAGAGCG CATGTCTAAA CCCGGGGCTG TCTCAACTCC TGTAAAACAT
    GTAGATGATC ATACACCCAA AGCAGTGGAA GAAGTTACAG TTGAAAAACA TGAGAAGCAG
    ACACCCACTC TTCCAGAACC AAAACCTGTA TATGCTCAAG TCGGACAGCC AGATGTGGAT
    TTACCTGTCA GCCCTTCTGA TGGTGTTCTG CCAAATTCAA CACATGAAGA TGGGATTCTT
    AGGCCCAGCA TGAAACTGGT AAAATTCAGA AAAGGAGATA GTGTGGGTTT GCGACTAGCT
    GGTGGAAATG ATGTCGGAAT ATTTGTAGCT GGCGTTCTAG AAGATAGCCC TGCAGCCAAA
    GAAGGCTTAG AGGAAGGTGA TCAAATTCTC AGGGTGAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCTGTTCT TTTCCTCCTC GACCTCCCTA AAGGTGAAGA AGTGACCATA
    TTGGCTCAGA AGAAAAAGGA CGTTTATCGC CGCATTGTAG AGTCAGATGT AGGAGACTCG
    TTCTATATTA GAACACATTT TGAATATGAG AAAGAATCTC CTTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGGGT CGTGGACACC CTGTACAACG GGAAGCTGGG CTCCTGGCTG
    GCCATTCGAA TTGGCAAAAA TCACAAGGAG GTAGAGCGAG GCATCGTTCC TAATAAGAAC
    AGAGCCGAGC AGTTAGCCAG TGTACAGTAC ACACTTCCAA AGACAGCAGG TGGCGATCGG
    GCAGACTTCT GGAGGTTTCG AGGTCTTCGT AGCTCCAAGA GAAATCTTCG AAAAAGCAGA
    GAGGACCTGT CAGCGCAGCC AGTTCAAACA AAGTTCCCAG CTTATGAAAG GGTTGTTCTT
    CGAGAAGCTG GATTCCTAAG ACCTGTAACC ATCTTTGGAC CGATTGCTGA TGTTGCCAGA
    GAAAAGTTAG CAAGGGAGGA GCCAGACATT TATCAGATTG CAAAAAGTGA ACCACGAGAT
    GCTGGAACTG ACCATCGGAG CTCGGGCATT ATTCGCCTTC ATACAATAAA GCAAATCATA
    GATCAAGATA AACATGCTTT ATTAGATGTC ACACCAAATG CAGTCGATCG CCTTAATTAT
    GCACAGTGGT ATCCGATTGT TGTGTTCCTT AACCCTGACT CTAAGCAGGG TGTAAAAACA
    ATGAGGATGA GGCTATGTCC AGAGTCTCGG AAAAGTGCCA GGAAGCTATA TGAACGGTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACCA CAATTAACTT AAACTCAATG
    AATGATGGTT GGTATGGTGC CCTGAAAGAA GCGATTCAGC AGCAACAGAA CCAGCTGGTG
    TGGGTCTCTG AGGGGAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAGTACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGATACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAAACTC TTAATGATGA GGTGGGGACT CCACCGGAGT CTGCTATTAC ACGGTCCTCT
    GAGCCTGTAA GAGAGGATTC CTCTGGAATG CATCATGAAA ACCAAACATA TCCTCCTTAC
    TCACCACAAG CGCAGCCACA AGCTATTCAT AGAATAGACT CCCCTGGACT TAAGACAGCC
    TCTCAACAGG TATATAGGAA GGAGCCATAT CCTGAGGAAA TGATGAGACA AAACCATATT
    TTAAAACAGC CAGCTCTTGG TCATCCAGGG CAGAGGCTGG ATAAAGAGCC AAATCCAGCC
    TATGATCCCC AACTTCCATA TGTAGAAAAA CAAGCCAGCA GAGACCTTGA GCAGCCACCA
    TACAGGTATG AGTCCTCAAG CTACACAGAC CAGTTTTCTC GGAACTATGA CCATCGCCTA
    CGATTTGAAG ACCGAGTCCC TACCTATGAA GACCAGTGGT CATATTATGA TGACAAACAG
    CCCTACCCAA CTCGGCCCTT TGATACTCAG CATCCTCGAG ACTTGGACTC CAGACAGCAT
    CCTGAAGAGG CTTCAGAACG AGGCTATTTC CAGCGTTTTG AAGAGCCCGC CCCTCTGCCA
    TATGACAGTA GACCACGCTA CGAGCAGCTG CCTCGAACCT CTACTCTCCG ACATGAAGAA
    CAGCCAACCA GTGGATATGA GGTGCACAAC AGGTACAGGC CAGAAGCACA GCCCTATGCT
    CCAGCAGGTC CTAAGTCATC TGAGCCGAAG CAGTACTTTG ACCAGTACCC ACGAAGTTAT
    GAGCAAGTAC CACCACCAGG ATTTACCTCC AAAACAGGCC ATTACGAGCC TCTCCATGGT
    GCTGCAGTTG TTCCTCCTCT GATACCTTCC TCTCAACATA AGCCAGAAGT TCTGCCCTCA
    GCTACCAAGC CACAGCCTCC GCCCCCAGCC CTAACTGAGG AAGAGGAGGA TCCAGCAATG
    AAACCACAGT CTGTGCTCAC CAGGGTCAAA ATGTTTGAAA ACAAAAGATC TGCGTCTCTG
    GAGAACAAGA AGGATGTGAA TGACACTGCC AGCTTTAAGC CTCCAGAAGT AGCATCTAAA
    CCTCCAAGTG CTTCTCTTGT TGGCCCTAAA CCTGTTTCTC AGACTCAGTT TAGTGAGCAT
    GACAAAACAC TCTACAGGCT CCCAGAGCCT CAGAAACCTC AAGCGAAGCC ACCTGAAGAT
    ATTGTTCGAT CAAATCATTA CGATCCTGAA GAGGATGAAG AGTATTACCG GAAACAGCTC
    TCTTACTTTG ACCGGAGAAG TTTCGAGAGC AAGCCTCCTG CACATATTCC TGCTGGCCAT
    CACTCAGAGC CTGCCAAGCC AGTCCATTCT CAGAGTCAGC CGAATTTCTC TAGTTATTCT
    TCAAAGGGGA AACCCGAAAC TGATGCTATG GATAGATCAT TCAGTGAGAA ACGTTATGAT
    CCAACCCAGG CCATGCCTCC TCCTCCTCCG TTGCCCTCAC AGTACAGCCA GCCAGTTCCG
    CCTCTGTCCA ACTCTTCTCT CCACATACAT TCCAAGGCCG CCCAGAGTGA AGGCAATTCC
    GTATCGTTGG ATTTTCAGAA CTCATATATA TCCAAACCAG ACCCACCCCC ATCTCAGAGC
    AAACCAGCAA CTTTTAGACC ACCAACCCGG GAGGACCCTC CTCAGACCTT CTATCCCCAG
    AAAAGTTTCC CAGACAAAGC TTCAGTTAAT GGAGCTGAGC AGACTCAGAA AACCATCACT
    CCAGCATACA ACCGATTCAC ACCAAAGCCG TACACGAGCT CTGCCCGGCC ATTTGAACGC
    AAGTTTGAAA GTCCAAAGTT CAACCATAAT CTTCTGCCAA GTGAAACTGT ACATAAACCT
    GAATTGTCTT CAAAACCTCC CCCTTCTCCA AAAACTCTCA TGAAGGCTCA TAGTTCCACA
    CAGCCGCCTG AGTTTGACAG TGGAGTCGAG ACTTTCTCTG TTCACACAGA TAAGCCTAAA
    TATCAAATAA ATAATATCAG CACCATGCCT AAAGCTGTTC CTGTGAGTCC TTCAGCTGTG
    GAAGAAGATG AAGATGAGGA TGGTCATACT GTAGTGGCTA CAGCCCGCGG CATTTTTAAC
    AGCAATGGCG GTGTGTTGAG TTCCATAGAA ACTGGTGTTA GTATAATTAT CCCACAAGGA
    GCCATTCCTG AAGGAATTGA GCAAGAAATC TATTTCAAAG TCTGCAGAGA CAATAGCATC
    CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACTCTGCTGA GCCCCCTAGT GATGTGCGGG
    CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTACGCT TGCCACACTG TGACCCTAAA
    ACTTGGCAAA ACAAGTGTCT TCCTGGAGAT CCGAATTACC TTGTTGGAGC CAACTGTGTT
    TCTGTCCTGA TTGACCACTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa13314
    Clone ID Related Accession (Same CDS sequence) NM_001106266.1
    Accession Version NM_001106266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473980.1. On or before Oct 4, 2007 this sequence version replaced XM_001058833.1, XM_218747.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099736.1
    CDS12..5213
    Translation

    Target ORF information:

    RefSeq Version NM_001106266.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001106266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa22874
    Clone ID Related Accession (Same CDS sequence) XM_006229330.3
    Accession Version XM_006229330.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTCTACG GATCAAAATT GCAGAGCACA GCAATGGAGG AAACAGCTAT ATGGGAACAG 
    CACACAGTGA CGCTTCACAG GGCTCCTGGG TTTGGATTTG GAATTGCAAT ATCTGGTGGA
    AGAGATAATC CTCATTTTCA GAGTGGGGAA ACCTCCATAG TGATTTCTGA TGTGCTAAAA
    GGAGGGCCAG CTGAAGGACA GCTACAGGAA AATGACCGAG TCGCAATGGT TAACGGAGTT
    TCAATGGATA ATGTTGAACA TGCTTTTGCT GTTCAGCAGC TAAGGAAAAG TGGGAAAAAC
    GCAAAAATTA CCATCCGAAG AAAGAAGAAA GTTCAGATTC CTGTAAGTCA CCCAGACCCT
    GACCCAGTGT CTGATAATGA AGATGATAGC TATGACGAGG ATGTGCACGA TCCAAGAAGT
    GGCCGAGGTG CCCTAGCTAA CAGAAGGGGT GAGAAGAGCT GGGCAAGGGA TAGAAGCGCA
    AGCAGGGACC GGAGCCTGTC CCCTCGCTCA GACAGGCGAT CAGTGGCCTC CAGTCAGCCC
    GCCAAACCCA CCAAGGTCAC ATTGGTGAAG TCTCGGAAAA ATGAAGAATA TGGTCTTCGA
    TTGGCCAGCC ATATATTTGT AAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGT
    AACATTCAAG AAGGGGATGT TGTCCTGAAG ATAAATGGTA CTGTGACAGA AAATATGTCA
    CTGACAGATG CAAAAACATT GATAGAAAGG TCTAAAGGCA AGTTAAAAAT GGTAGTGCAA
    AGAGATGAGC GGGCTACCTT ATTGAATGTC CCTGATCTTT CAGATAGTAT CCATTCTGCT
    AATGCCTCTG AAAGAGATGA CATTTCAGAA ATTCAGTCAC TAGCATCAGA CCATTCAGTT
    CGCTCCCATG ACAGGCCTCC CCGCCGAAGC CAGTCACGAT CTCCCGACCA ACGTTCAGAG
    CCCTCTGATC ATTCCACACA GTCTCCACAG CAGCCCAGCA ATGGCAGTCT CCGGAGCAGA
    GAGGAAGAGC GCATGTCTAA ACCCGGGGCT GTCTCAACTC CTGTAAAACA TGTAGATGAT
    CATACACCCA AAGCAGTGGA AGAAGTTACA GTTGAAAAAC ATGAGAAGCA GACACCCACT
    CTTCCAGAAC CAAAACCTGT ATATGCTCAA GTCGGACAGC CAGATGTGGA TTTACCTGTC
    AGCCCTTCTG ATGGTGTTCT GCCAAATTCA ACACATGAAG ATGGGATTCT TAGGCCCAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGTT TGCGACTAGC TGGTGGAAAT
    GATGTCGGAA TATTTGTAGC TGGCGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA
    GAGGAAGGTG ATCAAATTCT CAGGGTGAAC AATGTAGATT TCACAAATAT CATAAGAGAA
    GAAGCTGTTC TTTTCCTCCT CGACCTCCCT AAAGGTGAAG AAGTGACCAT ATTGGCTCAG
    AAGAAAAAGG ACGTTTATCG CCGCATTGTA GAGTCAGATG TAGGAGACTC GTTCTATATT
    AGAACACATT TTGAATATGA GAAAGAATCT CCTTATGGAC TCAGTTTTAA CAAAGGAGAG
    GTGTTCCGGG TCGTGGACAC CCTGTACAAC GGGAAGCTGG GCTCCTGGCT GGCCATTCGA
    ATTGGCAAAA ATCACAAGGA GGTAGAGCGA GGCATCGTTC CTAATAAGAA CAGAGCCGAG
    CAGTTAGCCA GTGTACAGTA CACACTTCCA AAGACAGCAG GTGGCGATCG GGCAGACTTC
    TGGAGGTTTC GAGGTCTTCG TAGCTCCAAG AGAAATCTTC GAAAAAGCAG AGAGGACCTG
    TCAGCGCAGC CAGTTCAAAC AAAGTTCCCA GCTTATGAAA GGGTTGTTCT TCGAGAAGCT
    GGATTCCTAA GACCTGTAAC CATCTTTGGA CCGATTGCTG ATGTTGCCAG AGAAAAGTTA
    GCAAGGGAGG AGCCAGACAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGAACT
    GACCATCGGA GCTCGGGCAT TATTCGCCTT CATACAATAA AGCAAATCAT AGATCAAGAT
    AAACATGCTT TATTAGATGT CACACCAAAT GCAGTCGATC GCCTTAATTA TGCACAGTGG
    TATCCGATTG TTGTGTTCCT TAACCCTGAC TCTAAGCAGG GTGTAAAAAC AATGAGGATG
    AGGCTATGTC CAGAGTCTCG GAAAAGTGCC AGGAAGCTAT ATGAACGGTC TCATAAACTT
    CGTAAAAATA ATCACCATCT TTTTACAACC ACAATTAACT TAAACTCAAT GAATGATGGT
    TGGTATGGTG CCCTGAAAGA AGCGATTCAG CAGCAACAGA ACCAGCTGGT GTGGGTCTCT
    GAGGGGAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC
    TACCTGTCAG CCCCAGGTAG TGAGTACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT
    GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT
    CTTAATGATG AGGTGGGGAC TCCACCGGAG TCTGCTATTA CACGGTCCTC TGAGCCTGTA
    AGAGAGGATT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA CTCACCACAA
    GCGCAGCCAC AAGCTATTCA TAGAATAGAC TCCCCTGGAC TTAAGACAGC CTCTCAACAG
    AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAAACCC AGCATCATCA
    GCCTCTGCAG TTAAGCATAA TGTAAATTTA ACTAATGTCA ACCTGGAGGA GCCCACCCCA
    GCTCCTCCCA CCTCGCACGT ATCACAAGCT GATTGTTTAG GAGCACCAAG TCCTGAGGCA
    CCTCACACGA TGCTCAGAGA CGAAGGAGTG TCATTGCCGT CGCATGTAGA CCCAGCAAAG
    GTATATAGGA AGGAGCCATA TCCTGAGGAA ATGATGAGAC AAAACCATAT TTTAAAACAG
    CCAGCTCTTG GTCATCCAGG GCAGAGGCTG GATAAAGAGC CAAATCCAGC CTATGATCCC
    CAACTTCCAT ATGTAGAAAA ACAAGCCAGC AGAGACCTTG AGCAGCCACC ATACAGGTAT
    GAGTCCTCAA GCTACACAGA CCAGTTTTCT CGGAACTATG ACCATCGCCT ACGATTTGAA
    GACCGAGTCC CTACCTATGA AGACCAGTGG TCATATTATG ATGACAAACA GCCCTACCCA
    ACTCGGCCCT TTGATACTCA GCATCCTCGA GACTTGGACT CCAGACAGCA TCCTGAAGAG
    GCTTCAGAAC GAGGCTATTT CCAGCGTTTT GAAGAGCCCG CCCCTCTGCC ATATGACAGT
    AGACCACGCT ACGAGCAGCT GCCTCGAACC TCTACTCTCC GACATGAAGA ACAGCCAACC
    AGTGGATATG AGGTGCACAA CAGGTACAGG CCAGAAGCAC AGCCCTATGC TCCAGCAGGT
    CCTAAGTCAT CTGAGCCGAA GCAGTACTTT GACCAGTACC CACGAAGTTA TGAGCAAGTA
    CCACCACCAG GATTTACCTC CAAAACAGGC CATTACGAGC CTCTCCATGG TGCTGCAGTT
    GTTCCTCCTC TGATACCTTC CTCTCAACAT AAGCCAGAAG TTCTGCCCTC AGCTACCAAG
    CCACAGCCTC CGCCCCCAGC CCTAACTGAG GAAGAGGAGG ATCCAGCAAT GAAACCACAG
    TCTGTGCTCA CCAGGGTCAA AATGTTTGAA AACAAAAGAT CTGCGTCTCT GGAGAACAAG
    AAGGATGTGA ATGACACTGC CAGCTTTAAG CCTCCAGAAG TAGCATCTAA ACCTCCAAGT
    GCTTCTCTTG TTGGCCCTAA ACCTGTTTCT CAGACTCAGT TTAGTGAGCA TGACAAAACA
    CTCTACAGGC TCCCAGAGCC TCAGAAACCT CAAGCGAAGC CACCTGAAGA TATTGTTCGA
    TCAAATCATT ACGATCCTGA AGAGGATGAA GAGTATTACC GGAAACAGCT CTCTTACTTT
    GACCGGAGAA GTTTCGAGAG CAAGCCTCCT GCACATATTC CTGCTGGCCA TCACTCAGAG
    CCTGCCAAGC CAGTCCATTC TCAGAGTCAG CCGAATTTCT CTAGTTATTC TTCAAAGGGG
    AAACCCGAAA CTGATGCTAT GGATAGATCA TTCAGTGAGA AACGTTATGA TCCAACCCAG
    GCCATGCCTC CTCCTCCTCC GTTGCCCTCA CAGTACAGCC AGCCAGTTCC GCCTCTGTCC
    AACTCTTCTC TCCACATACA TTCCAAGGCC GCCCAGAGTG AAGGCAATTC CGTATCGTTG
    GATTTTCAGA ACTCATATAT ATCCAAACCA GACCCACCCC CATCTCAGAG CAAACCAGCA
    ACTTTTAGAC CACCAACCCG GGAGGACCCT CCTCAGACCT TCTATCCCCA GAAAAGTTTC
    CCAGACAAAG CTTCAGTTAA TGGAGCTGAG CAGACTCAGA AAACCATCAC TCCAGCATAC
    AACCGATTCA CACCAAAGCC GTACACGAGC TCTGCCCGGC CATTTGAACG CAAGTTTGAA
    AGTCCAAAGT TCAACCATAA TCTTCTGCCA AGTGAAACTG TACATAAACC TGAATTGTCT
    TCAAAACCTC CCCCTTCTCC AAAAACTCTC ATGAAGGCTC ATAGTTCCAC ACAGCCGCCT
    GAGTTTGACA GTGGAGTCGA GACTTTCTCT GTTCACACAG ATAAGCCTAA ATATCAAATA
    AATAATATCA GCACCATGCC TAAAGCTGTT CCTGTGAGTC CTTCAGCTGT GGAAGAAGAT
    GAAGATGAGG ATGGTCATAC TGTAGTGGCT ACAGCCCGCG GCATTTTTAA CAGCAATGGC
    GGTGTGTTGA GTTCCATAGA AACTGGTGTT AGTATAATTA TCCCACAAGG AGCCATTCCT
    GAAGGAATTG AGCAAGAAAT CTATTTCAAA GTCTGCAGAG ACAATAGCAT CCTCCCACCT
    TTAGATAAAG AGAAAGGTGA AACTCTGCTG AGCCCCCTAG TGATGTGCGG GCCCCATGGC
    CTCAAGTTCC TGAAGCCTGT GGAGCTACGC TTGCCACACT GTGCGTCCAT GACTCCTGAC
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGACCCTAA AACTTGGCAA
    AACAAGTGTC TTCCTGGAGA TCCGAATTAC CTTGTTGGAG CCAACTGTGT TTCTGTCCTG
    ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006229392.1
    CDS112..5406
    Translation

    Target ORF information:

    RefSeq Version XM_006229330.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006229330.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTCTACG GATCAAAATT GCAGAGCACA GCAATGGAGG AAACAGCTAT ATGGGAACAG 
    CACACAGTGA CGCTTCACAG GGCTCCTGGG TTTGGATTTG GAATTGCAAT ATCTGGTGGA
    AGAGATAATC CTCATTTTCA GAGTGGGGAA ACCTCCATAG TGATTTCTGA TGTGCTAAAA
    GGAGGGCCAG CTGAAGGACA GCTACAGGAA AATGACCGAG TCGCAATGGT TAACGGAGTT
    TCAATGGATA ATGTTGAACA TGCTTTTGCT GTTCAGCAGC TAAGGAAAAG TGGGAAAAAC
    GCAAAAATTA CCATCCGAAG AAAGAAGAAA GTTCAGATTC CTGTAAGTCA CCCAGACCCT
    GACCCAGTGT CTGATAATGA AGATGATAGC TATGACGAGG ATGTGCACGA TCCAAGAAGT
    GGCCGAGGTG CCCTAGCTAA CAGAAGGGGT GAGAAGAGCT GGGCAAGGGA TAGAAGCGCA
    AGCAGGGACC GGAGCCTGTC CCCTCGCTCA GACAGGCGAT CAGTGGCCTC CAGTCAGCCC
    GCCAAACCCA CCAAGGTCAC ATTGGTGAAG TCTCGGAAAA ATGAAGAATA TGGTCTTCGA
    TTGGCCAGCC ATATATTTGT AAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGT
    AACATTCAAG AAGGGGATGT TGTCCTGAAG ATAAATGGTA CTGTGACAGA AAATATGTCA
    CTGACAGATG CAAAAACATT GATAGAAAGG TCTAAAGGCA AGTTAAAAAT GGTAGTGCAA
    AGAGATGAGC GGGCTACCTT ATTGAATGTC CCTGATCTTT CAGATAGTAT CCATTCTGCT
    AATGCCTCTG AAAGAGATGA CATTTCAGAA ATTCAGTCAC TAGCATCAGA CCATTCAGTT
    CGCTCCCATG ACAGGCCTCC CCGCCGAAGC CAGTCACGAT CTCCCGACCA ACGTTCAGAG
    CCCTCTGATC ATTCCACACA GTCTCCACAG CAGCCCAGCA ATGGCAGTCT CCGGAGCAGA
    GAGGAAGAGC GCATGTCTAA ACCCGGGGCT GTCTCAACTC CTGTAAAACA TGTAGATGAT
    CATACACCCA AAGCAGTGGA AGAAGTTACA GTTGAAAAAC ATGAGAAGCA GACACCCACT
    CTTCCAGAAC CAAAACCTGT ATATGCTCAA GTCGGACAGC CAGATGTGGA TTTACCTGTC
    AGCCCTTCTG ATGGTGTTCT GCCAAATTCA ACACATGAAG ATGGGATTCT TAGGCCCAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGTT TGCGACTAGC TGGTGGAAAT
    GATGTCGGAA TATTTGTAGC TGGCGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA
    GAGGAAGGTG ATCAAATTCT CAGGGTGAAC AATGTAGATT TCACAAATAT CATAAGAGAA
    GAAGCTGTTC TTTTCCTCCT CGACCTCCCT AAAGGTGAAG AAGTGACCAT ATTGGCTCAG
    AAGAAAAAGG ACGTTTATCG CCGCATTGTA GAGTCAGATG TAGGAGACTC GTTCTATATT
    AGAACACATT TTGAATATGA GAAAGAATCT CCTTATGGAC TCAGTTTTAA CAAAGGAGAG
    GTGTTCCGGG TCGTGGACAC CCTGTACAAC GGGAAGCTGG GCTCCTGGCT GGCCATTCGA
    ATTGGCAAAA ATCACAAGGA GGTAGAGCGA GGCATCGTTC CTAATAAGAA CAGAGCCGAG
    CAGTTAGCCA GTGTACAGTA CACACTTCCA AAGACAGCAG GTGGCGATCG GGCAGACTTC
    TGGAGGTTTC GAGGTCTTCG TAGCTCCAAG AGAAATCTTC GAAAAAGCAG AGAGGACCTG
    TCAGCGCAGC CAGTTCAAAC AAAGTTCCCA GCTTATGAAA GGGTTGTTCT TCGAGAAGCT
    GGATTCCTAA GACCTGTAAC CATCTTTGGA CCGATTGCTG ATGTTGCCAG AGAAAAGTTA
    GCAAGGGAGG AGCCAGACAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGAACT
    GACCATCGGA GCTCGGGCAT TATTCGCCTT CATACAATAA AGCAAATCAT AGATCAAGAT
    AAACATGCTT TATTAGATGT CACACCAAAT GCAGTCGATC GCCTTAATTA TGCACAGTGG
    TATCCGATTG TTGTGTTCCT TAACCCTGAC TCTAAGCAGG GTGTAAAAAC AATGAGGATG
    AGGCTATGTC CAGAGTCTCG GAAAAGTGCC AGGAAGCTAT ATGAACGGTC TCATAAACTT
    CGTAAAAATA ATCACCATCT TTTTACAACC ACAATTAACT TAAACTCAAT GAATGATGGT
    TGGTATGGTG CCCTGAAAGA AGCGATTCAG CAGCAACAGA ACCAGCTGGT GTGGGTCTCT
    GAGGGGAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC
    TACCTGTCAG CCCCAGGTAG TGAGTACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT
    GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT
    CTTAATGATG AGGTGGGGAC TCCACCGGAG TCTGCTATTA CACGGTCCTC TGAGCCTGTA
    AGAGAGGATT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA CTCACCACAA
    GCGCAGCCAC AAGCTATTCA TAGAATAGAC TCCCCTGGAC TTAAGACAGC CTCTCAACAG
    AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAAACCC AGCATCATCA
    GCCTCTGCAG TTAAGCATAA TGTAAATTTA ACTAATGTCA ACCTGGAGGA GCCCACCCCA
    GCTCCTCCCA CCTCGCACGT ATCACAAGCT GATTGTTTAG GAGCACCAAG TCCTGAGGCA
    CCTCACACGA TGCTCAGAGA CGAAGGAGTG TCATTGCCGT CGCATGTAGA CCCAGCAAAG
    GTATATAGGA AGGAGCCATA TCCTGAGGAA ATGATGAGAC AAAACCATAT TTTAAAACAG
    CCAGCTCTTG GTCATCCAGG GCAGAGGCTG GATAAAGAGC CAAATCCAGC CTATGATCCC
    CAACTTCCAT ATGTAGAAAA ACAAGCCAGC AGAGACCTTG AGCAGCCACC ATACAGGTAT
    GAGTCCTCAA GCTACACAGA CCAGTTTTCT CGGAACTATG ACCATCGCCT ACGATTTGAA
    GACCGAGTCC CTACCTATGA AGACCAGTGG TCATATTATG ATGACAAACA GCCCTACCCA
    ACTCGGCCCT TTGATACTCA GCATCCTCGA GACTTGGACT CCAGACAGCA TCCTGAAGAG
    GCTTCAGAAC GAGGCTATTT CCAGCGTTTT GAAGAGCCCG CCCCTCTGCC ATATGACAGT
    AGACCACGCT ACGAGCAGCT GCCTCGAACC TCTACTCTCC GACATGAAGA ACAGCCAACC
    AGTGGATATG AGGTGCACAA CAGGTACAGG CCAGAAGCAC AGCCCTATGC TCCAGCAGGT
    CCTAAGTCAT CTGAGCCGAA GCAGTACTTT GACCAGTACC CACGAAGTTA TGAGCAAGTA
    CCACCACCAG GATTTACCTC CAAAACAGGC CATTACGAGC CTCTCCATGG TGCTGCAGTT
    GTTCCTCCTC TGATACCTTC CTCTCAACAT AAGCCAGAAG TTCTGCCCTC AGCTACCAAG
    CCACAGCCTC CGCCCCCAGC CCTAACTGAG GAAGAGGAGG ATCCAGCAAT GAAACCACAG
    TCTGTGCTCA CCAGGGTCAA AATGTTTGAA AACAAAAGAT CTGCGTCTCT GGAGAACAAG
    AAGGATGTGA ATGACACTGC CAGCTTTAAG CCTCCAGAAG TAGCATCTAA ACCTCCAAGT
    GCTTCTCTTG TTGGCCCTAA ACCTGTTTCT CAGACTCAGT TTAGTGAGCA TGACAAAACA
    CTCTACAGGC TCCCAGAGCC TCAGAAACCT CAAGCGAAGC CACCTGAAGA TATTGTTCGA
    TCAAATCATT ACGATCCTGA AGAGGATGAA GAGTATTACC GGAAACAGCT CTCTTACTTT
    GACCGGAGAA GTTTCGAGAG CAAGCCTCCT GCACATATTC CTGCTGGCCA TCACTCAGAG
    CCTGCCAAGC CAGTCCATTC TCAGAGTCAG CCGAATTTCT CTAGTTATTC TTCAAAGGGG
    AAACCCGAAA CTGATGCTAT GGATAGATCA TTCAGTGAGA AACGTTATGA TCCAACCCAG
    GCCATGCCTC CTCCTCCTCC GTTGCCCTCA CAGTACAGCC AGCCAGTTCC GCCTCTGTCC
    AACTCTTCTC TCCACATACA TTCCAAGGCC GCCCAGAGTG AAGGCAATTC CGTATCGTTG
    GATTTTCAGA ACTCATATAT ATCCAAACCA GACCCACCCC CATCTCAGAG CAAACCAGCA
    ACTTTTAGAC CACCAACCCG GGAGGACCCT CCTCAGACCT TCTATCCCCA GAAAAGTTTC
    CCAGACAAAG CTTCAGTTAA TGGAGCTGAG CAGACTCAGA AAACCATCAC TCCAGCATAC
    AACCGATTCA CACCAAAGCC GTACACGAGC TCTGCCCGGC CATTTGAACG CAAGTTTGAA
    AGTCCAAAGT TCAACCATAA TCTTCTGCCA AGTGAAACTG TACATAAACC TGAATTGTCT
    TCAAAACCTC CCCCTTCTCC AAAAACTCTC ATGAAGGCTC ATAGTTCCAC ACAGCCGCCT
    GAGTTTGACA GTGGAGTCGA GACTTTCTCT GTTCACACAG ATAAGCCTAA ATATCAAATA
    AATAATATCA GCACCATGCC TAAAGCTGTT CCTGTGAGTC CTTCAGCTGT GGAAGAAGAT
    GAAGATGAGG ATGGTCATAC TGTAGTGGCT ACAGCCCGCG GCATTTTTAA CAGCAATGGC
    GGTGTGTTGA GTTCCATAGA AACTGGTGTT AGTATAATTA TCCCACAAGG AGCCATTCCT
    GAAGGAATTG AGCAAGAAAT CTATTTCAAA GTCTGCAGAG ACAATAGCAT CCTCCCACCT
    TTAGATAAAG AGAAAGGTGA AACTCTGCTG AGCCCCCTAG TGATGTGCGG GCCCCATGGC
    CTCAAGTTCC TGAAGCCTGT GGAGCTACGC TTGCCACACT GTGCGTCCAT GACTCCTGAC
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGACCCTAA AACTTGGCAA
    AACAAGTGTC TTCCTGGAGA TCCGAATTAC CTTGTTGGAG CCAACTGTGT TTCTGTCCTG
    ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa22875
    Clone ID Related Accession (Same CDS sequence) XM_006229331.2 , XM_017588936.1 , XM_006229333.2
    Accession Version XM_006229331.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006229331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006229393.1
    CDS244..5505
    Translation

    Target ORF information:

    RefSeq Version XM_006229331.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006229331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa22875
    Clone ID Related Accession (Same CDS sequence) XM_006229331.2 , XM_017588936.1 , XM_006229333.2
    Accession Version XM_017588936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444425.1
    CDS246..5507
    Translation

    Target ORF information:

    RefSeq Version XM_017588936.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa22875
    Clone ID Related Accession (Same CDS sequence) XM_006229331.2 , XM_017588936.1 , XM_006229333.2
    Accession Version XM_006229333.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006229333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006229395.1
    CDS54..5315
    Translation

    Target ORF information:

    RefSeq Version XM_006229333.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006229333.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTCG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACCA TCCGAAGAAA GAAGAAAGTT
    CAGATTCCTG TAAGTCACCC AGACCCTGAC CCAGTGTCTG ATAATGAAGA TGATAGCTAT
    GACGAGGATG TGCACGATCC AAGAAGTGGC CGAGGTGCCC TAGCTAACAG AAGGGGTGAG
    AAGAGCTGGG CAAGGGATAG AAGCGCAAGC AGGGACCGGA GCCTGTCCCC TCGCTCAGAC
    AGGCGATCAG TGGCCTCCAG TCAGCCCGCC AAACCCACCA AGGTCACATT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CCTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCACTG ACAGATGCAA AAACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTATT GAATGTCCCT
    GATCTTTCAG ATAGTATCCA TTCTGCTAAT GCCTCTGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGTTCGC TCCCATGACA GGCCTCCCCG CCGAAGCCAG
    TCACGATCTC CCGACCAACG TTCAGAGCCC TCTGATCATT CCACACAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGCA TGTCTAAACC CGGGGCTGTC
    TCAACTCCTG TAAAACATGT AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAAAAACATG AGAAGCAGAC ACCCACTCTT CCAGAACCAA AACCTGTATA TGCTCAAGTC
    GGACAGCCAG ATGTGGATTT ACCTGTCAGC CCTTCTGATG GTGTTCTGCC AAATTCAACA
    CATGAAGATG GGATTCTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGAGAAGAA GCTGTTCTTT TCCTCCTCGA CCTCCCTAAA
    GGTGAAGAAG TGACCATATT GGCTCAGAAG AAAAAGGACG TTTATCGCCG CATTGTAGAG
    TCAGATGTAG GAGACTCGTT CTATATTAGA ACACATTTTG AATATGAGAA AGAATCTCCT
    TATGGACTCA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGACACCCT GTACAACGGG
    AAGCTGGGCT CCTGGCTGGC CATTCGAATT GGCAAAAATC ACAAGGAGGT AGAGCGAGGC
    ATCGTTCCTA ATAAGAACAG AGCCGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCAGGTG GCGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGTAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACCTGTCA GCGCAGCCAG TTCAAACAAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCG
    ATTGCTGATG TTGCCAGAGA AAAGTTAGCA AGGGAGGAGC CAGACATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CATCGGAGCT CGGGCATTAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAAGATAAA CATGCTTTAT TAGATGTCAC ACCAAATGCA
    GTCGATCGCC TTAATTATGC ACAGTGGTAT CCGATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAGGGTG TAAAAACAAT GAGGATGAGG CTATGTCCAG AGTCTCGGAA AAGTGCCAGG
    AAGCTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACCACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TATGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC ACCGGAGTCT
    GCTATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAGCC TCTGCAGTTA AGCATAATGT AAATTTAACT
    AATGTCAACC TGGAGGAGCC CACCCCAGCT CCTCCCACCT CGCACGTATC ACAAGCTGAT
    TGTTTAGGAG CACCAAGTCC TGAGGCACCT CACACGATGC TCAGAGACGA AGGAGTGTCA
    TTGCCGTCGC ATGTAGACCC AGCAAAGGTA TATAGGAAGG AGCCATATCC TGAGGAAATG
    ATGAGACAAA ACCATATTTT AAAACAGCCA GCTCTTGGTC ATCCAGGGCA GAGGCTGGAT
    AAAGAGCCAA ATCCAGCCTA TGATCCCCAA CTTCCATATG TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCACCATA CAGGTATGAG TCCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG ATTTGAAGAC CGAGTCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCCAACT CGGCCCTTTG ATACTCAGCA TCCTCGAGAC
    TTGGACTCCA GACAGCATCC TGAAGAGGCT TCAGAACGAG GCTATTTCCA GCGTTTTGAA
    GAGCCCGCCC CTCTGCCATA TGACAGTAGA CCACGCTACG AGCAGCTGCC TCGAACCTCT
    ACTCTCCGAC ATGAAGAACA GCCAACCAGT GGATATGAGG TGCACAACAG GTACAGGCCA
    GAAGCACAGC CCTATGCTCC AGCAGGTCCT AAGTCATCTG AGCCGAAGCA GTACTTTGAC
    CAGTACCCAC GAAGTTATGA GCAAGTACCA CCACCAGGAT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTT CCTCCTCTGA TACCTTCCTC TCAACATAAG
    CCAGAAGTTC TGCCCTCAGC TACCAAGCCA CAGCCTCCGC CCCCAGCCCT AACTGAGGAA
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GGGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTCTGGA GAACAAGAAG GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGTTG GCCCTAAACC TGTTTCTCAG
    ACTCAGTTTA GTGAGCATGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GCGAAGCCAC CTGAAGATAT TGTTCGATCA AATCATTACG ATCCTGAAGA GGATGAAGAG
    TATTACCGGA AACAGCTCTC TTACTTTGAC CGGAGAAGTT TCGAGAGCAA GCCTCCTGCA
    CATATTCCTG CTGGCCATCA CTCAGAGCCT GCCAAGCCAG TCCATTCTCA GAGTCAGCCG
    AATTTCTCTA GTTATTCTTC AAAGGGGAAA CCCGAAACTG ATGCTATGGA TAGATCATTC
    AGTGAGAAAC GTTATGATCC AACCCAGGCC ATGCCTCCTC CTCCTCCGTT GCCCTCACAG
    TACAGCCAGC CAGTTCCGCC TCTGTCCAAC TCTTCTCTCC ACATACATTC CAAGGCCGCC
    CAGAGTGAAG GCAATTCCGT ATCGTTGGAT TTTCAGAACT CATATATATC CAAACCAGAC
    CCACCCCCAT CTCAGAGCAA ACCAGCAACT TTTAGACCAC CAACCCGGGA GGACCCTCCT
    CAGACCTTCT ATCCCCAGAA AAGTTTCCCA GACAAAGCTT CAGTTAATGG AGCTGAGCAG
    ACTCAGAAAA CCATCACTCC AGCATACAAC CGATTCACAC CAAAGCCGTA CACGAGCTCT
    GCCCGGCCAT TTGAACGCAA GTTTGAAAGT CCAAAGTTCA ACCATAATCT TCTGCCAAGT
    GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAACCTCCCC CTTCTCCAAA AACTCTCATG
    AAGGCTCATA GTTCCACACA GCCGCCTGAG TTTGACAGTG GAGTCGAGAC TTTCTCTGTT
    CACACAGATA AGCCTAAATA TCAAATAAAT AATATCAGCA CCATGCCTAA AGCTGTTCCT
    GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA
    GCCCGCGGCA TTTTTAACAG CAATGGCGGT GTGTTGAGTT CCATAGAAAC TGGTGTTAGT
    ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAGC AAGAAATCTA TTTCAAAGTC
    TGCAGAGACA ATAGCATCCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTGAGC
    CCCCTAGTGA TGTGCGGGCC CCATGGCCTC AAGTTCCTGA AGCCTGTGGA GCTACGCTTG
    CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC
    TCGTCGGGTG ACCCTAAAAC TTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT
    GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa22876
    Clone ID Related Accession (Same CDS sequence) XM_006229332.3
    Accession Version XM_006229332.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCTCAGTG GCCGGGTCCG GCAGGTTTTG CGTGGCCGCC TGAGTTGCCC GCGACGGCTC 
    TGCCCGCCGA CGGCACGTCT CTCGGCGGTC GCGCGTTCCG GGGAAGTTAC GTGCGGGAGC
    AGGGTTTTGA GGAGACGCCC GAAGGCGGAG GGGACAGCCG GAGGCCCCGG TACTGCGGAG
    CGGCGAGCGG GCCGAGGGCG GCGGAGGCCG AGCCGGCGGC CGGGTGTGCC CCGCGGAGAA
    GCCCGGGCGG GGCGGACGCT TCCCGGACTT TTGTCCCACT TGAATCCCCT CCCCGTGGGC
    CGGGCCTTTC CGGCCTCCCC CGCCCCTGCC CCGCTGTCCC CGGGAGATGT TTATGCGGAC
    GGTGGCGTGA GGAGCGGGCG GGCCGGCGGC GCGGAGTTTC GGGTCCGAGG AGCTTCGCGC
    GGCGCGGAGA GAGACAAGAT GTCCGCCAGG GCCGCGGCCG CTAAGAGCAC AGCAATGGAG
    GAAACAGCTA TATGGGAACA GCACACAGTG ACGCTTCACA GGGCTCCTGG GTTTGGATTT
    GGAATTGCAA TATCTGGTGG AAGAGATAAT CCTCATTTTC AGAGTGGGGA AACCTCCATA
    GTGATTTCTG ATGTGCTAAA AGGAGGGCCA GCTGAAGGAC AGCTACAGGA AAATGACCGA
    GTCGCAATGG TTAACGGAGT TTCAATGGAT AATGTTGAAC ATGCTTTTGC TGTTCAGCAG
    CTAAGGAAAA GTGGGAAAAA CGCAAAAATT ACCATCCGAA GAAAGAAGAA AGTTCAGATT
    CCTGTAAGTC ACCCAGACCC TGACCCAGTG TCTGATAATG AAGATGATAG CTATGACGAG
    GATGTGCACG ATCCAAGAAG TGGCCGAGGT GCCCTAGCTA ACAGAAGGGG TGAGAAGAGC
    TGGGCAAGGG ATAGAAGCGC AAGCAGGGAC CGGAGCCTGT CCCCTCGCTC AGACAGGCGA
    TCAGTGGCCT CCAGTCAGCC CGCCAAACCC ACCAAGGTCA CATTGGTGAA GTCTCGGAAA
    AATGAAGAAT ATGGTCTTCG ATTGGCCAGC CATATATTTG TAAAGGAAAT TTCACAAGAT
    AGTTTGGCAG CAAGAGATGG TAACATTCAA GAAGGGGATG TTGTCCTGAA GATAAATGGT
    ACTGTGACAG AAAATATGTC ACTGACAGAT GCAAAAACAT TGATAGAAAG GTCTAAAGGC
    AAGTTAAAAA TGGTAGTGCA AAGAGATGAG CGGGCTACCT TATTGAATGT CCCTGATCTT
    TCAGATAGTA TCCATTCTGC TAATGCCTCT GAAAGAGATG ACATTTCAGA AATTCAGTCA
    CTAGCATCAG ACCATTCAGT TCGCTCCCAT GACAGGCCTC CCCGCCGAAG CCAGTCACGA
    TCTCCCGACC AACGTTCAGA GCCCTCTGAT CATTCCACAC AGTCTCCACA GCAGCCCAGC
    AATGGCAGTC TCCGGAGCAG AGAGGAAGAG CGCATGTCTA AACCCGGGGC TGTCTCAACT
    CCTGTAAAAC ATGTAGATGA TCATACACCC AAAGCAGTGG AAGAAGTTAC AGTTGAAAAA
    CATGAGAAGC AGACACCCAC TCTTCCAGAA CCAAAACCTG TATATGCTCA AGTCGGACAG
    CCAGATGTGG ATTTACCTGT CAGCCCTTCT GATGGTGTTC TGCCAAATTC AACACATGAA
    GATGGGATTC TTAGGCCCAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGT
    TTGCGACTAG CTGGTGGAAA TGATGTCGGA ATATTTGTAG CTGGCGTTCT AGAAGATAGC
    CCTGCAGCCA AAGAAGGCTT AGAGGAAGGT GATCAAATTC TCAGGGTGAA CAATGTAGAT
    TTCACAAATA TCATAAGAGA AGAAGCTGTT CTTTTCCTCC TCGACCTCCC TAAAGGTGAA
    GAAGTGACCA TATTGGCTCA GAAGAAAAAG GACGTTTATC GCCGCATTGT AGAGTCAGAT
    GTAGGAGACT CGTTCTATAT TAGAACACAT TTTGAATATG AGAAAGAATC TCCTTATGGA
    CTCAGTTTTA ACAAAGGAGA GGTGTTCCGG GTCGTGGACA CCCTGTACAA CGGGAAGCTG
    GGCTCCTGGC TGGCCATTCG AATTGGCAAA AATCACAAGG AGGTAGAGCG AGGCATCGTT
    CCTAATAAGA ACAGAGCCGA GCAGTTAGCC AGTGTACAGT ACACACTTCC AAAGACAGCA
    GGTGGCGATC GGGCAGACTT CTGGAGGTTT CGAGGTCTTC GTAGCTCCAA GAGAAATCTT
    CGAAAAAGCA GAGAGGACCT GTCAGCGCAG CCAGTTCAAA CAAAGTTCCC AGCTTATGAA
    AGGGTTGTTC TTCGAGAAGC TGGATTCCTA AGACCTGTAA CCATCTTTGG ACCGATTGCT
    GATGTTGCCA GAGAAAAGTT AGCAAGGGAG GAGCCAGACA TTTATCAGAT TGCAAAAAGT
    GAACCACGAG ATGCTGGAAC TGACCATCGG AGCTCGGGCA TTATTCGCCT TCATACAATA
    AAGCAAATCA TAGATCAAGA TAAACATGCT TTATTAGATG TCACACCAAA TGCAGTCGAT
    CGCCTTAATT ATGCACAGTG GTATCCGATT GTTGTGTTCC TTAACCCTGA CTCTAAGCAG
    GGTGTAAAAA CAATGAGGAT GAGGCTATGT CCAGAGTCTC GGAAAAGTGC CAGGAAGCTA
    TATGAACGGT CTCATAAACT TCGTAAAAAT AATCACCATC TTTTTACAAC CACAATTAAC
    TTAAACTCAA TGAATGATGG TTGGTATGGT GCCCTGAAAG AAGCGATTCA GCAGCAACAG
    AACCAGCTGG TGTGGGTCTC TGAGGGGAAG GCGGATGGTG CTACAAGTGA TGACCTTGAT
    TTGCATGATG ATCGTCTGTC CTACCTGTCA GCCCCAGGTA GTGAGTACTC AATGTATAGC
    ACGGACAGTA GACACACTTC TGACTATGAA GACACAGATA CAGAAGGCGG GGCCTACACT
    GATCAAGAAC TAGATGAAAC TCTTAATGAT GAGGTGGGGA CTCCACCGGA GTCTGCTATT
    ACACGGTCCT CTGAGCCTGT AAGAGAGGAT TCCTCTGGAA TGCATCATGA AAACCAAACA
    TATCCTCCTT ACTCACCACA AGCGCAGCCA CAAGCTATTC ATAGAATAGA CTCCCCTGGA
    CTTAAGACAG CCTCTCAACA GAAAGCAGAA GCCTCATCTC CAGTCCCTTA CCTTTCGCCT
    GAAACAAACC CAGCATCATC AGCCTCTGCA GTTAAGCATA ATGTAAATTT AACTAATGTC
    AACCTGGAGG AGCCCACCCC AGCTCCTCCC ACCTCGCACG TATCACAAGC TGATTGTTTA
    GGAGCACCAA GTCCTGAGGC ACCTCACACG ATGCTCAGAG ACGAAGGAGT GTCATTGCCG
    TCGCATGTAG ACCCAGCAAA GGTATATAGG AAGGAGCCAT ATCCTGAGGA AATGATGAGA
    CAAAACCATA TTTTAAAACA GCCAGCTCTT GGTCATCCAG GGCAGAGGCT GGATAAAGAG
    CCAAATCCAG CCTATGATCC CCAACTTCCA TATGTAGAAA AACAAGCCAG CAGAGACCTT
    GAGCAGCCAC CATACAGGTA TGAGTCCTCA AGCTACACAG ACCAGTTTTC TCGGAACTAT
    GACCATCGCC TACGATTTGA AGACCGAGTC CCTACCTATG AAGACCAGTG GTCATATTAT
    GATGACAAAC AGCCCTACCC AACTCGGCCC TTTGATACTC AGCATCCTCG AGACTTGGAC
    TCCAGACAGC ATCCTGAAGA GGCTTCAGAA CGAGGCTATT TCCAGCGTTT TGAAGAGCCC
    GCCCCTCTGC CATATGACAG TAGACCACGC TACGAGCAGC TGCCTCGAAC CTCTACTCTC
    CGACATGAAG AACAGCCAAC CAGTGGATAT GAGGTGCACA ACAGGTACAG GCCAGAAGCA
    CAGCCCTATG CTCCAGCAGG TCCTAAGTCA TCTGAGCCGA AGCAGTACTT TGACCAGTAC
    CCACGAAGTT ATGAGCAAGT ACCACCACCA GGATTTACCT CCAAAACAGG CCATTACGAG
    CCTCTCCATG GTGCTGCAGT TGTTCCTCCT CTGATACCTT CCTCTCAACA TAAGCCAGAA
    GTTCTGCCCT CAGCTACCAA GCCACAGCCT CCGCCCCCAG CCCTAACTGA GGAAGAGGAG
    GATCCAGCAA TGAAACCACA GTCTGTGCTC ACCAGGGTCA AAATGTTTGA AAACAAAAGA
    TCTGCGTCTC TGGAGAACAA GAAGGATGTG AATGACACTG CCAGCTTTAA GCCTCCAGAA
    GTAGCATCTA AACCTCCAAG TGCTTCTCTT GTTGGCCCTA AACCTGTTTC TCAGACTCAG
    TTTAGTGAGC ATGACAAAAC ACTCTACAGG CTCCCAGAGC CTCAGAAACC TCAAGCGAAG
    CCACCTGAAG ATATTGTTCG ATCAAATCAT TACGATCCTG AAGAGGATGA AGAGTATTAC
    CGGAAACAGC TCTCTTACTT TGACCGGAGA AGTTTCGAGA GCAAGCCTCC TGCACATATT
    CCTGCTGGCC ATCACTCAGA GCCTGCCAAG CCAGTCCATT CTCAGAGTCA GCCGAATTTC
    TCTAGTTATT CTTCAAAGGG GAAACCCGAA ACTGATGCTA TGGATAGATC ATTCAGTGAG
    AAACGTTATG ATCCAACCCA GGCCATGCCT CCTCCTCCTC CGTTGCCCTC ACAGTACAGC
    CAGCCAGTTC CGCCTCTGTC CAACTCTTCT CTCCACATAC ATTCCAAGGC CGCCCAGAGT
    GAAGGCAATT CCGTATCGTT GGATTTTCAG AACTCATATA TATCCAAACC AGACCCACCC
    CCATCTCAGA GCAAACCAGC AACTTTTAGA CCACCAACCC GGGAGGACCC TCCTCAGACC
    TTCTATCCCC AGAAAAGTTT CCCAGACAAA GCTTCAGTTA ATGGAGCTGA GCAGACTCAG
    AAAACCATCA CTCCAGCATA CAACCGATTC ACACCAAAGC CGTACACGAG CTCTGCCCGG
    CCATTTGAAC GCAAGTTTGA AAGTCCAAAG TTCAACCATA ATCTTCTGCC AAGTGAAACT
    GTACATAAAC CTGAATTGTC TTCAAAACCT CCCCCTTCTC CAAAAACTCT CATGAAGGCT
    CATAGTTCCA CACAGCCGCC TGAGTTTGAC AGTGGAGTCG AGACTTTCTC TGTTCACACA
    GATAAGCCTA AATATCAAAT AAATAATATC AGCACCATGC CTAAAGCTGT TCCTGTGAGT
    CCTTCAGCTG TGGAAGAAGA TGAAGATGAG GATGGTCATA CTGTAGTGGC TACAGCCCGC
    GGCATTTTTA ACAGCAATGG CGGTGTGTTG AGTTCCATAG AAACTGGTGT TAGTATAATT
    ATCCCACAAG GAGCCATTCC TGAAGGAATT GAGCAAGAAA TCTATTTCAA AGTCTGCAGA
    GACAATAGCA TCCTCCCACC TTTAGATAAA GAGAAAGGTG AAACTCTGCT GAGCCCCCTA
    GTGATGTGCG GGCCCCATGG CCTCAAGTTC CTGAAGCCTG TGGAGCTACG CTTGCCACAC
    TGTGCGTCCA TGACTCCTGA CGGTTGGTCT TTTGCTCTAA AATCATCCGA CTCCTCGTCG
    GGTGACCCTA AAACTTGGCA AAACAAGTGT CTTCCTGGAG ATCCGAATTA CCTTGTTGGA
    GCCAACTGTG TTTCTGTCCT GATTGACCAC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006229394.1
    CDS74..5809
    Translation

    Target ORF information:

    RefSeq Version XM_006229332.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006229332.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCTCAGTG GCCGGGTCCG GCAGGTTTTG CGTGGCCGCC TGAGTTGCCC GCGACGGCTC 
    TGCCCGCCGA CGGCACGTCT CTCGGCGGTC GCGCGTTCCG GGGAAGTTAC GTGCGGGAGC
    AGGGTTTTGA GGAGACGCCC GAAGGCGGAG GGGACAGCCG GAGGCCCCGG TACTGCGGAG
    CGGCGAGCGG GCCGAGGGCG GCGGAGGCCG AGCCGGCGGC CGGGTGTGCC CCGCGGAGAA
    GCCCGGGCGG GGCGGACGCT TCCCGGACTT TTGTCCCACT TGAATCCCCT CCCCGTGGGC
    CGGGCCTTTC CGGCCTCCCC CGCCCCTGCC CCGCTGTCCC CGGGAGATGT TTATGCGGAC
    GGTGGCGTGA GGAGCGGGCG GGCCGGCGGC GCGGAGTTTC GGGTCCGAGG AGCTTCGCGC
    GGCGCGGAGA GAGACAAGAT GTCCGCCAGG GCCGCGGCCG CTAAGAGCAC AGCAATGGAG
    GAAACAGCTA TATGGGAACA GCACACAGTG ACGCTTCACA GGGCTCCTGG GTTTGGATTT
    GGAATTGCAA TATCTGGTGG AAGAGATAAT CCTCATTTTC AGAGTGGGGA AACCTCCATA
    GTGATTTCTG ATGTGCTAAA AGGAGGGCCA GCTGAAGGAC AGCTACAGGA AAATGACCGA
    GTCGCAATGG TTAACGGAGT TTCAATGGAT AATGTTGAAC ATGCTTTTGC TGTTCAGCAG
    CTAAGGAAAA GTGGGAAAAA CGCAAAAATT ACCATCCGAA GAAAGAAGAA AGTTCAGATT
    CCTGTAAGTC ACCCAGACCC TGACCCAGTG TCTGATAATG AAGATGATAG CTATGACGAG
    GATGTGCACG ATCCAAGAAG TGGCCGAGGT GCCCTAGCTA ACAGAAGGGG TGAGAAGAGC
    TGGGCAAGGG ATAGAAGCGC AAGCAGGGAC CGGAGCCTGT CCCCTCGCTC AGACAGGCGA
    TCAGTGGCCT CCAGTCAGCC CGCCAAACCC ACCAAGGTCA CATTGGTGAA GTCTCGGAAA
    AATGAAGAAT ATGGTCTTCG ATTGGCCAGC CATATATTTG TAAAGGAAAT TTCACAAGAT
    AGTTTGGCAG CAAGAGATGG TAACATTCAA GAAGGGGATG TTGTCCTGAA GATAAATGGT
    ACTGTGACAG AAAATATGTC ACTGACAGAT GCAAAAACAT TGATAGAAAG GTCTAAAGGC
    AAGTTAAAAA TGGTAGTGCA AAGAGATGAG CGGGCTACCT TATTGAATGT CCCTGATCTT
    TCAGATAGTA TCCATTCTGC TAATGCCTCT GAAAGAGATG ACATTTCAGA AATTCAGTCA
    CTAGCATCAG ACCATTCAGT TCGCTCCCAT GACAGGCCTC CCCGCCGAAG CCAGTCACGA
    TCTCCCGACC AACGTTCAGA GCCCTCTGAT CATTCCACAC AGTCTCCACA GCAGCCCAGC
    AATGGCAGTC TCCGGAGCAG AGAGGAAGAG CGCATGTCTA AACCCGGGGC TGTCTCAACT
    CCTGTAAAAC ATGTAGATGA TCATACACCC AAAGCAGTGG AAGAAGTTAC AGTTGAAAAA
    CATGAGAAGC AGACACCCAC TCTTCCAGAA CCAAAACCTG TATATGCTCA AGTCGGACAG
    CCAGATGTGG ATTTACCTGT CAGCCCTTCT GATGGTGTTC TGCCAAATTC AACACATGAA
    GATGGGATTC TTAGGCCCAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGT
    TTGCGACTAG CTGGTGGAAA TGATGTCGGA ATATTTGTAG CTGGCGTTCT AGAAGATAGC
    CCTGCAGCCA AAGAAGGCTT AGAGGAAGGT GATCAAATTC TCAGGGTGAA CAATGTAGAT
    TTCACAAATA TCATAAGAGA AGAAGCTGTT CTTTTCCTCC TCGACCTCCC TAAAGGTGAA
    GAAGTGACCA TATTGGCTCA GAAGAAAAAG GACGTTTATC GCCGCATTGT AGAGTCAGAT
    GTAGGAGACT CGTTCTATAT TAGAACACAT TTTGAATATG AGAAAGAATC TCCTTATGGA
    CTCAGTTTTA ACAAAGGAGA GGTGTTCCGG GTCGTGGACA CCCTGTACAA CGGGAAGCTG
    GGCTCCTGGC TGGCCATTCG AATTGGCAAA AATCACAAGG AGGTAGAGCG AGGCATCGTT
    CCTAATAAGA ACAGAGCCGA GCAGTTAGCC AGTGTACAGT ACACACTTCC AAAGACAGCA
    GGTGGCGATC GGGCAGACTT CTGGAGGTTT CGAGGTCTTC GTAGCTCCAA GAGAAATCTT
    CGAAAAAGCA GAGAGGACCT GTCAGCGCAG CCAGTTCAAA CAAAGTTCCC AGCTTATGAA
    AGGGTTGTTC TTCGAGAAGC TGGATTCCTA AGACCTGTAA CCATCTTTGG ACCGATTGCT
    GATGTTGCCA GAGAAAAGTT AGCAAGGGAG GAGCCAGACA TTTATCAGAT TGCAAAAAGT
    GAACCACGAG ATGCTGGAAC TGACCATCGG AGCTCGGGCA TTATTCGCCT TCATACAATA
    AAGCAAATCA TAGATCAAGA TAAACATGCT TTATTAGATG TCACACCAAA TGCAGTCGAT
    CGCCTTAATT ATGCACAGTG GTATCCGATT GTTGTGTTCC TTAACCCTGA CTCTAAGCAG
    GGTGTAAAAA CAATGAGGAT GAGGCTATGT CCAGAGTCTC GGAAAAGTGC CAGGAAGCTA
    TATGAACGGT CTCATAAACT TCGTAAAAAT AATCACCATC TTTTTACAAC CACAATTAAC
    TTAAACTCAA TGAATGATGG TTGGTATGGT GCCCTGAAAG AAGCGATTCA GCAGCAACAG
    AACCAGCTGG TGTGGGTCTC TGAGGGGAAG GCGGATGGTG CTACAAGTGA TGACCTTGAT
    TTGCATGATG ATCGTCTGTC CTACCTGTCA GCCCCAGGTA GTGAGTACTC AATGTATAGC
    ACGGACAGTA GACACACTTC TGACTATGAA GACACAGATA CAGAAGGCGG GGCCTACACT
    GATCAAGAAC TAGATGAAAC TCTTAATGAT GAGGTGGGGA CTCCACCGGA GTCTGCTATT
    ACACGGTCCT CTGAGCCTGT AAGAGAGGAT TCCTCTGGAA TGCATCATGA AAACCAAACA
    TATCCTCCTT ACTCACCACA AGCGCAGCCA CAAGCTATTC ATAGAATAGA CTCCCCTGGA
    CTTAAGACAG CCTCTCAACA GAAAGCAGAA GCCTCATCTC CAGTCCCTTA CCTTTCGCCT
    GAAACAAACC CAGCATCATC AGCCTCTGCA GTTAAGCATA ATGTAAATTT AACTAATGTC
    AACCTGGAGG AGCCCACCCC AGCTCCTCCC ACCTCGCACG TATCACAAGC TGATTGTTTA
    GGAGCACCAA GTCCTGAGGC ACCTCACACG ATGCTCAGAG ACGAAGGAGT GTCATTGCCG
    TCGCATGTAG ACCCAGCAAA GGTATATAGG AAGGAGCCAT ATCCTGAGGA AATGATGAGA
    CAAAACCATA TTTTAAAACA GCCAGCTCTT GGTCATCCAG GGCAGAGGCT GGATAAAGAG
    CCAAATCCAG CCTATGATCC CCAACTTCCA TATGTAGAAA AACAAGCCAG CAGAGACCTT
    GAGCAGCCAC CATACAGGTA TGAGTCCTCA AGCTACACAG ACCAGTTTTC TCGGAACTAT
    GACCATCGCC TACGATTTGA AGACCGAGTC CCTACCTATG AAGACCAGTG GTCATATTAT
    GATGACAAAC AGCCCTACCC AACTCGGCCC TTTGATACTC AGCATCCTCG AGACTTGGAC
    TCCAGACAGC ATCCTGAAGA GGCTTCAGAA CGAGGCTATT TCCAGCGTTT TGAAGAGCCC
    GCCCCTCTGC CATATGACAG TAGACCACGC TACGAGCAGC TGCCTCGAAC CTCTACTCTC
    CGACATGAAG AACAGCCAAC CAGTGGATAT GAGGTGCACA ACAGGTACAG GCCAGAAGCA
    CAGCCCTATG CTCCAGCAGG TCCTAAGTCA TCTGAGCCGA AGCAGTACTT TGACCAGTAC
    CCACGAAGTT ATGAGCAAGT ACCACCACCA GGATTTACCT CCAAAACAGG CCATTACGAG
    CCTCTCCATG GTGCTGCAGT TGTTCCTCCT CTGATACCTT CCTCTCAACA TAAGCCAGAA
    GTTCTGCCCT CAGCTACCAA GCCACAGCCT CCGCCCCCAG CCCTAACTGA GGAAGAGGAG
    GATCCAGCAA TGAAACCACA GTCTGTGCTC ACCAGGGTCA AAATGTTTGA AAACAAAAGA
    TCTGCGTCTC TGGAGAACAA GAAGGATGTG AATGACACTG CCAGCTTTAA GCCTCCAGAA
    GTAGCATCTA AACCTCCAAG TGCTTCTCTT GTTGGCCCTA AACCTGTTTC TCAGACTCAG
    TTTAGTGAGC ATGACAAAAC ACTCTACAGG CTCCCAGAGC CTCAGAAACC TCAAGCGAAG
    CCACCTGAAG ATATTGTTCG ATCAAATCAT TACGATCCTG AAGAGGATGA AGAGTATTAC
    CGGAAACAGC TCTCTTACTT TGACCGGAGA AGTTTCGAGA GCAAGCCTCC TGCACATATT
    CCTGCTGGCC ATCACTCAGA GCCTGCCAAG CCAGTCCATT CTCAGAGTCA GCCGAATTTC
    TCTAGTTATT CTTCAAAGGG GAAACCCGAA ACTGATGCTA TGGATAGATC ATTCAGTGAG
    AAACGTTATG ATCCAACCCA GGCCATGCCT CCTCCTCCTC CGTTGCCCTC ACAGTACAGC
    CAGCCAGTTC CGCCTCTGTC CAACTCTTCT CTCCACATAC ATTCCAAGGC CGCCCAGAGT
    GAAGGCAATT CCGTATCGTT GGATTTTCAG AACTCATATA TATCCAAACC AGACCCACCC
    CCATCTCAGA GCAAACCAGC AACTTTTAGA CCACCAACCC GGGAGGACCC TCCTCAGACC
    TTCTATCCCC AGAAAAGTTT CCCAGACAAA GCTTCAGTTA ATGGAGCTGA GCAGACTCAG
    AAAACCATCA CTCCAGCATA CAACCGATTC ACACCAAAGC CGTACACGAG CTCTGCCCGG
    CCATTTGAAC GCAAGTTTGA AAGTCCAAAG TTCAACCATA ATCTTCTGCC AAGTGAAACT
    GTACATAAAC CTGAATTGTC TTCAAAACCT CCCCCTTCTC CAAAAACTCT CATGAAGGCT
    CATAGTTCCA CACAGCCGCC TGAGTTTGAC AGTGGAGTCG AGACTTTCTC TGTTCACACA
    GATAAGCCTA AATATCAAAT AAATAATATC AGCACCATGC CTAAAGCTGT TCCTGTGAGT
    CCTTCAGCTG TGGAAGAAGA TGAAGATGAG GATGGTCATA CTGTAGTGGC TACAGCCCGC
    GGCATTTTTA ACAGCAATGG CGGTGTGTTG AGTTCCATAG AAACTGGTGT TAGTATAATT
    ATCCCACAAG GAGCCATTCC TGAAGGAATT GAGCAAGAAA TCTATTTCAA AGTCTGCAGA
    GACAATAGCA TCCTCCCACC TTTAGATAAA GAGAAAGGTG AAACTCTGCT GAGCCCCCTA
    GTGATGTGCG GGCCCCATGG CCTCAAGTTC CTGAAGCCTG TGGAGCTACG CTTGCCACAC
    TGTGCGTCCA TGACTCCTGA CGGTTGGTCT TTTGCTCTAA AATCATCCGA CTCCTCGTCG
    GGTGACCCTA AAACTTGGCA AAACAAGTGT CTTCCTGGAG ATCCGAATTA CCTTGTTGGA
    GCCAACTGTG TTTCTGTCCT GATTGACCAC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa56464
    Clone ID Related Accession (Same CDS sequence) XM_017588935.1
    Accession Version XM_017588935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGG 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCAATGTCA GCCAAGGGAA ACCTTCTCTA
    AGACGAATTA AAGGGAGACT ACATCGAAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGCTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTCGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAA AGTGGGAAAA ACGCAAAAAT TACCATCCGA
    AGAAAGAAGA AAGTTCAGAT TCCTGTAAGT CACCCAGACC CTGACCCAGT GTCTGATAAT
    GAAGATGATA GCTATGACGA GGATGTGCAC GATCCAAGAA GTGGCCGAGG TGCCCTAGCT
    AACAGAAGGG GTGAGAAGAG CTGGGCAAGG GATAGAAGCG CAAGCAGGGA CCGGAGCCTG
    TCCCCTCGCT CAGACAGGCG ATCAGTGGCC TCCAGTCAGC CCGCCAAACC CACCAAGGTC
    ACATTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCCTGA AGATAAATGG TACTGTGACA GAAAATATGT CACTGACAGA TGCAAAAACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTATTGAATG TCCCTGATCT TTCAGATAGT ATCCATTCTG CTAATGCCTC TGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG TTCGCTCCCA TGACAGGCCT
    CCCCGCCGAA GCCAGTCACG ATCTCCCGAC CAACGTTCAG AGCCCTCTGA TCATTCCACA
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGCATGTCT
    AAACCCGGGG CTGTCTCAAC TCCTGTAAAA CATGTAGATG ATCATACACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAAAA ACATGAGAAG CAGACACCCA CTCTTCCAGA ACCAAAACCT
    GTATATGCTC AAGTCGGACA GCCAGATGTG GATTTACCTG TCAGCCCTTC TGATGGTGTT
    CTGCCAAATT CAACACATGA AGATGGGATT CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGAG AAGAAGCTGT TCTTTTCCTC
    CTCGACCTCC CTAAAGGTGA AGAAGTGACC ATATTGGCTC AGAAGAAAAA GGACGTTTAT
    CGCCGCATTG TAGAGTCAGA TGTAGGAGAC TCGTTCTATA TTAGAACACA TTTTGAATAT
    GAGAAAGAAT CTCCTTATGG ACTCAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAC
    ACCCTGTACA ACGGGAAGCT GGGCTCCTGG CTGGCCATTC GAATTGGCAA AAATCACAAG
    GAGGTAGAGC GAGGCATCGT TCCTAATAAG AACAGAGCCG AGCAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC AGGTGGCGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGTAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACC TGTCAGCGCA GCCAGTTCAA
    ACAAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCGATTGC TGATGTTGCC AGAGAAAAGT TAGCAAGGGA GGAGCCAGAC
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCATCG GAGCTCGGGC
    ATTATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAAG ATAAACATGC TTTATTAGAT
    GTCACACCAA ATGCAGTCGA TCGCCTTAAT TATGCACAGT GGTATCCGAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA GGGTGTAAAA ACAATGAGGA TGAGGCTATG TCCAGAGTCT
    CGGAAAAGTG CCAGGAAGCT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CCACAATTAA CTTAAACTCA ATGAATGATG GTTGGTATGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCACCGG AGTCTGCTAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAGCCTCTGC AGTTAAGCAT
    AATGTAAATT TAACTAATGT CAACCTGGAG GAGCCCACCC CAGCTCCTCC CACCTCGCAC
    GTATCACAAG CTGATTGTTT AGGAGCACCA AGTCCTGAGG CACCTCACAC GATGCTCAGA
    GACGAAGGAG TGTCATTGCC GTCGCATGTA GACCCAGCAA AGGTATATAG GAAGGAGCCA
    TATCCTGAGG AAATGATGAG ACAAAACCAT ATTTTAAAAC AGCCAGCTCT TGGTCATCCA
    GGGCAGAGGC TGGATAAAGA GCCAAATCCA GCCTATGATC CCCAACTTCC ATATGTAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCA CCATACAGGT ATGAGTCCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGATTTG AAGACCGAGT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC CAACTCGGCC CTTTGATACT
    CAGCATCCTC GAGACTTGGA CTCCAGACAG CATCCTGAAG AGGCTTCAGA ACGAGGCTAT
    TTCCAGCGTT TTGAAGAGCC CGCCCCTCTG CCATATGACA GTAGACCACG CTACGAGCAG
    CTGCCTCGAA CCTCTACTCT CCGACATGAA GAACAGCCAA CCAGTGGATA TGAGGTGCAC
    AACAGGTACA GGCCAGAAGC ACAGCCCTAT GCTCCAGCAG GTCCTAAGTC ATCTGAGCCG
    AAGCAGTACT TTGACCAGTA CCCACGAAGT TATGAGCAAG TACCACCACC AGGATTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTTCCTCC TCTGATACCT
    TCCTCTCAAC ATAAGCCAGA AGTTCTGCCC TCAGCTACCA AGCCACAGCC TCCGCCCCCA
    GCCCTAACTG AGGAAGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGGGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT CTGGAGAACA AGAAGGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCAGA AGTAGCATCT AAACCTCCAA GTGCTTCTCT TGTTGGCCCT
    AAACCTGTTT CTCAGACTCA GTTTAGTGAG CATGACAAAA CACTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGCGAA GCCACCTGAA GATATTGTTC GATCAAATCA TTACGATCCT
    GAAGAGGATG AAGAGTATTA CCGGAAACAG CTCTCTTACT TTGACCGGAG AAGTTTCGAG
    AGCAAGCCTC CTGCACATAT TCCTGCTGGC CATCACTCAG AGCCTGCCAA GCCAGTCCAT
    TCTCAGAGTC AGCCGAATTT CTCTAGTTAT TCTTCAAAGG GGAAACCCGA AACTGATGCT
    ATGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAACCC AGGCCATGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGTT CCGCCTCTGT CCAACTCTTC TCTCCACATA
    CATTCCAAGG CCGCCCAGAG TGAAGGCAAT TCCGTATCGT TGGATTTTCA GAACTCATAT
    ATATCCAAAC CAGACCCACC CCCATCTCAG AGCAAACCAG CAACTTTTAG ACCACCAACC
    CGGGAGGACC CTCCTCAGAC CTTCTATCCC CAGAAAAGTT TCCCAGACAA AGCTTCAGTT
    AATGGAGCTG AGCAGACTCA GAAAACCATC ACTCCAGCAT ACAACCGATT CACACCAAAG
    CCGTACACGA GCTCTGCCCG GCCATTTGAA CGCAAGTTTG AAAGTCCAAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAACC TCCCCCTTCT
    CCAAAAACTC TCATGAAGGC TCATAGTTCC ACACAGCCGC CTGAGTTTGA CAGTGGAGTC
    GAGACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACCATG
    CCTAAAGCTG TTCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCCCG CGGCATTTTT AACAGCAATG GCGGTGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATCCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGC GGGCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACTTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444424.1
    CDS19..5595
    Translation

    Target ORF information:

    RefSeq Version XM_017588935.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017588935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGG 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCAATGTCA GCCAAGGGAA ACCTTCTCTA
    AGACGAATTA AAGGGAGACT ACATCGAAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGCTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTCGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAA AGTGGGAAAA ACGCAAAAAT TACCATCCGA
    AGAAAGAAGA AAGTTCAGAT TCCTGTAAGT CACCCAGACC CTGACCCAGT GTCTGATAAT
    GAAGATGATA GCTATGACGA GGATGTGCAC GATCCAAGAA GTGGCCGAGG TGCCCTAGCT
    AACAGAAGGG GTGAGAAGAG CTGGGCAAGG GATAGAAGCG CAAGCAGGGA CCGGAGCCTG
    TCCCCTCGCT CAGACAGGCG ATCAGTGGCC TCCAGTCAGC CCGCCAAACC CACCAAGGTC
    ACATTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCCTGA AGATAAATGG TACTGTGACA GAAAATATGT CACTGACAGA TGCAAAAACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTATTGAATG TCCCTGATCT TTCAGATAGT ATCCATTCTG CTAATGCCTC TGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG TTCGCTCCCA TGACAGGCCT
    CCCCGCCGAA GCCAGTCACG ATCTCCCGAC CAACGTTCAG AGCCCTCTGA TCATTCCACA
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGCATGTCT
    AAACCCGGGG CTGTCTCAAC TCCTGTAAAA CATGTAGATG ATCATACACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAAAA ACATGAGAAG CAGACACCCA CTCTTCCAGA ACCAAAACCT
    GTATATGCTC AAGTCGGACA GCCAGATGTG GATTTACCTG TCAGCCCTTC TGATGGTGTT
    CTGCCAAATT CAACACATGA AGATGGGATT CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGAG AAGAAGCTGT TCTTTTCCTC
    CTCGACCTCC CTAAAGGTGA AGAAGTGACC ATATTGGCTC AGAAGAAAAA GGACGTTTAT
    CGCCGCATTG TAGAGTCAGA TGTAGGAGAC TCGTTCTATA TTAGAACACA TTTTGAATAT
    GAGAAAGAAT CTCCTTATGG ACTCAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAC
    ACCCTGTACA ACGGGAAGCT GGGCTCCTGG CTGGCCATTC GAATTGGCAA AAATCACAAG
    GAGGTAGAGC GAGGCATCGT TCCTAATAAG AACAGAGCCG AGCAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC AGGTGGCGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGTAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACC TGTCAGCGCA GCCAGTTCAA
    ACAAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCGATTGC TGATGTTGCC AGAGAAAAGT TAGCAAGGGA GGAGCCAGAC
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCATCG GAGCTCGGGC
    ATTATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAAG ATAAACATGC TTTATTAGAT
    GTCACACCAA ATGCAGTCGA TCGCCTTAAT TATGCACAGT GGTATCCGAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA GGGTGTAAAA ACAATGAGGA TGAGGCTATG TCCAGAGTCT
    CGGAAAAGTG CCAGGAAGCT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CCACAATTAA CTTAAACTCA ATGAATGATG GTTGGTATGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCACCGG AGTCTGCTAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAGCCTCTGC AGTTAAGCAT
    AATGTAAATT TAACTAATGT CAACCTGGAG GAGCCCACCC CAGCTCCTCC CACCTCGCAC
    GTATCACAAG CTGATTGTTT AGGAGCACCA AGTCCTGAGG CACCTCACAC GATGCTCAGA
    GACGAAGGAG TGTCATTGCC GTCGCATGTA GACCCAGCAA AGGTATATAG GAAGGAGCCA
    TATCCTGAGG AAATGATGAG ACAAAACCAT ATTTTAAAAC AGCCAGCTCT TGGTCATCCA
    GGGCAGAGGC TGGATAAAGA GCCAAATCCA GCCTATGATC CCCAACTTCC ATATGTAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCA CCATACAGGT ATGAGTCCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGATTTG AAGACCGAGT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC CAACTCGGCC CTTTGATACT
    CAGCATCCTC GAGACTTGGA CTCCAGACAG CATCCTGAAG AGGCTTCAGA ACGAGGCTAT
    TTCCAGCGTT TTGAAGAGCC CGCCCCTCTG CCATATGACA GTAGACCACG CTACGAGCAG
    CTGCCTCGAA CCTCTACTCT CCGACATGAA GAACAGCCAA CCAGTGGATA TGAGGTGCAC
    AACAGGTACA GGCCAGAAGC ACAGCCCTAT GCTCCAGCAG GTCCTAAGTC ATCTGAGCCG
    AAGCAGTACT TTGACCAGTA CCCACGAAGT TATGAGCAAG TACCACCACC AGGATTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTTCCTCC TCTGATACCT
    TCCTCTCAAC ATAAGCCAGA AGTTCTGCCC TCAGCTACCA AGCCACAGCC TCCGCCCCCA
    GCCCTAACTG AGGAAGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGGGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT CTGGAGAACA AGAAGGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCAGA AGTAGCATCT AAACCTCCAA GTGCTTCTCT TGTTGGCCCT
    AAACCTGTTT CTCAGACTCA GTTTAGTGAG CATGACAAAA CACTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGCGAA GCCACCTGAA GATATTGTTC GATCAAATCA TTACGATCCT
    GAAGAGGATG AAGAGTATTA CCGGAAACAG CTCTCTTACT TTGACCGGAG AAGTTTCGAG
    AGCAAGCCTC CTGCACATAT TCCTGCTGGC CATCACTCAG AGCCTGCCAA GCCAGTCCAT
    TCTCAGAGTC AGCCGAATTT CTCTAGTTAT TCTTCAAAGG GGAAACCCGA AACTGATGCT
    ATGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAACCC AGGCCATGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGTT CCGCCTCTGT CCAACTCTTC TCTCCACATA
    CATTCCAAGG CCGCCCAGAG TGAAGGCAAT TCCGTATCGT TGGATTTTCA GAACTCATAT
    ATATCCAAAC CAGACCCACC CCCATCTCAG AGCAAACCAG CAACTTTTAG ACCACCAACC
    CGGGAGGACC CTCCTCAGAC CTTCTATCCC CAGAAAAGTT TCCCAGACAA AGCTTCAGTT
    AATGGAGCTG AGCAGACTCA GAAAACCATC ACTCCAGCAT ACAACCGATT CACACCAAAG
    CCGTACACGA GCTCTGCCCG GCCATTTGAA CGCAAGTTTG AAAGTCCAAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAACC TCCCCCTTCT
    CCAAAAACTC TCATGAAGGC TCATAGTTCC ACACAGCCGC CTGAGTTTGA CAGTGGAGTC
    GAGACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACCATG
    CCTAAAGCTG TTCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCCCG CGGCATTTTT AACAGCAATG GCGGTGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATCCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGC GGGCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACTTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.